Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0009530

Physical Location & Seq

Genomic Coordinates Reverse (-)

17515989 .. 17519107

Gene Structure 3119 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1194700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2301 bp

ATGGAGGGAAAAGTATATAAGTTTCTCCATGGAACTATTGAAGCTACCATCTTCCATGCCACACCTTACTCACCACCACCAATATTCCCCTTCAATTGTATTTTTGCAAACGGAAAGCCTGCATATGTGACCATCAAAATAGACAACAATAAAGTGGCAAAGACGACCCACGAACGCGACCGTGTGTGGAACCAAACCTTTCAAATCCTCTGTGCTCATCCGCCTGATTCGACCATCACCATTACCATGAAAACAAAATGCTCCATCTTGGGAAAATTTCAAATTCAGGCACATGAGATACTGAACGAAGCAAGTTTTATCAACGGTTTTCTCCCTCTTGTACTCGAAAATGGGAAGCCAAATCCAGAACTCAAGCTCCGGTTTATGTTATGGTTTAAGCCCGTGAAGTTCGAACCAACTTGGGGGACAATAACTGTTAATGGTGGATTCAAGGGGTTGAGGAATGCATCATTTCCACAACGATCCAACAGCCATGTCACCCTTTATCAAGATGCTCACCACTGCTCAACTTTCAACCCTACGCCGGAGTTTTGTGGGAGTCCGAGAAGACTATGGGAAGATGTATACAAAGCCATTGAGGGGGCAACACATTTGATTTACATTGCGGGCTGGTCTTTCAATCCTAAGATGGTTCTGGTCAGGGATCCTCAAACAGACATCCCGCGTGCGCGAGGGGTGAAGCTCGGTGAATTACTAAAGCAGAAGGCCGAGGAAGGTGTGGCAGTGAGAATAATGCTTTGGGATGATCAAACTTCATTAAAAATTATCAAGAACAAAGGAATAATGGGAACACATGATGAAGATGCATTTTGTTACTTTGCACACACGAAAGTGATATGCAAATTGTGCCCTAGATTTCACCGCAAGTTCCCCACAATCTTCTCTCACCATCAGAAAACCATAACTGTAGACATCAAATCCCTCACCAGTGCAAGTGACAAAGAAATCATGAGCTTTGTTGGGGGTCTAGATCTTTGTGATGGGCGCTACGACACGGAGCAACATTCGTTGTTTCAAACACTCAATACAGAATCACATTGTGCTGATTTCTACCAGACCAACATTTCCGGGGCTAGCCTTCAGAGAGGAGGGCCAAGAACGCCGTGGCATGATGCTCACGCTTGTATAACCGGCGAGGCTGCTTGGGATGTTCTAACAAATTTCGAGCAACGATGGTCCAAGAAATGTGATCCCTCTTTATTGGTCCCTTCTATCACCTTAACAAGCCTCATTCAACAGACTTATGCAAGTAAACCCTCTGAGAGGGGCTGGAATGTTCAAGTTCTTAGGTCAATTGACAATGTTTCGGCAAGCCAATTGTTTCGAAACCTAACCGTGGAACATAGCATTCATGAAGCCTACGTTGAAGCAATTAGGCGCGCCGATAAGTTTCTATACATCGAGAACCAGTATTTCATGGGAGGGTGTTATCTGTGGGAGAACGATCAGCATAGTGGATGTAGAAACTTGATCCCCGTTGAAATTGCACTAAAAGTGGTCAATAAGATCAAAGAAAAGGAGAGGTTTGCAGTGTATATCGTGATTCCAATGTGGCCAGAAGGGTTACCTGAGAGTGAACCTGTGCAGGATATACTTCATTGGACTAGAAAGACAATGTCAATGATGTATAGATTGATTGGAGAAGCCATAAAAGAAAGTGGAGTTGAAGGGCATCACCCAAGAGACTATTTGAACTTCTATTGCCTTGCCAATAGAGAAGTTGAGACCCAAGGGGAGTTTGTTCCCCCTCATTCTCCTCATCATGCAACACAATATTGGAATGCACAAAAGCATAGGAGGTTCATGGTCTATGTTCACTCCAAGCTCATGATAGTGGACGATGCATACCTTATCCTAGGATCTGCAAACATAAACCAAAGATCCATGGACGGACATCGAGACACTGAGATCGCCATTGGATGCTACCAGTCCGACAACGGTGACAACAAGATGATGGTGAGCTCGGGAGGCAACATTGGAGCATACCGAATGTCATTATGGTACGAACACACAGGTCGTGTTGAAGAACTGTTCAAGCAGCCCGAAAGCTTAGAATGCGTGCAGAAGATGCGTTTTATCGGAGACCGGATGTGGGAAATTTACAGTGGGGAAGAAGTGGTCGACATGGAAGGTGCTCATCTTGTGACATATCCCATGAGTGTTTCAGAGGAAGGATATGTTGAGGACCTTGTGAAAGGCAGTGATGGTCATTTTCCAGATACAAAGGCTATGGTGAAGGGCAAGAGATCAAAAGTGATACCATCAATATTTACTACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004620 GO:0004630 GO:0005575 GO:0005623 GO:0005886 GO:0006629 GO:0006644 GO:0006793 GO:0006796 GO:0006808 GO:0006950 GO:0006970 GO:0006995 GO:0007154 GO:0007275 GO:0008081 GO:0008150 GO:0008152 GO:0009056 GO:0009267 GO:0009395 GO:0009605 GO:0009628 GO:0009791 GO:0009825 GO:0009987 GO:0009991 GO:0016020 GO:0016036 GO:0016042 GO:0016049 GO:0016298 GO:0016787 GO:0016788 GO:0019637 GO:0022622 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0033554 GO:0040007 GO:0042578 GO:0042594 GO:0043562 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044464 GO:0045848 GO:0046434 GO:0048364 GO:0048518 GO:0048731 GO:0048856 GO:0050789 GO:0050896 GO:0051301 GO:0051365 GO:0051716 GO:0065007 GO:0071496 GO:0071704 GO:0071944 GO:0099402 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

C2 PLD_C PLDc

Protein Analysis

767

Amino Acids

87.51

Weight (kDa)

7.04

Isoelectric Point (pI)

37.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PLDc_2	PF13091	608 - 646	6.8e-06	PLD-like domain
PLDc	PF00614	612 - 635	4.7e-06	Phospholipase D Active site motif
PLD_C	PF12357	682 - 755	1.5e-23	Phospholipase D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009530)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55180 AT1G55180
fragaria_vesca	FvH4_6g33140
malus_domestica	MD09G1194700.v1.1 MD17G1175200.v1.1
prunus_persica	Prupe.3G051200_v2.0.a1
pyrus_communis	pycom09g11160
rosa_chinensis	RchiOBHm_Chr2g0142831
rosa_laevigata	RLG00000019989
rosa_multiflora	Rmu_co8241083.1_g000001 Rmu_co8446503.1_g000001 Rmu_sc0012306.1_g000009 Rmu_sc0013116.1_g000004
rosa_roxburghii	Rroxscaffold_2G00102280 Rroxscaffold_2G00103320
rosa_rugosa	Rorug02G0375600 Rorug02G0375700
rosa_samantha	Rh2AG428800 Rh2BG438100 Rh2CG414300 Rh2DG447900
rosa_wichuraiana	Rw2G035060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	3 cut(s)	585, 930, 2142
AccII	CGCG	4 cut(s)	177, 685, 691, 1401
AciI	CCGC	4 cut(s)	221, 626, 683, 883
AclWI	GGATC	7 cut(s)	477, 659, 672, 1205, 1486, 1888, 1896
AcoI	YGGCCR	1 cut(s)	1574
AcsI	RAATTY	4 cut(s)	275, 282, 1180, 2118
AcuI	CTGAAG	1 cut(s)	1085
AdeI	CACNNNGTG	1 cut(s)	1061
AfaI	GTAC	2 cut(s)	342, 2024
AfiI	CCNNNNNNNGG	6 cut(s)	422, 544, 600, 1284, 1357, 2113
AjuI	GAANNNNNNNTTGG	2 cut(s)	742, 774
AleI	CACNNNNGTG	1 cut(s)	851
AloI	GAACNNNNNNTCC	2 cut(s)	360, 392
AluBI	AGCT	7 cut(s)	44, 376, 703, 975, 1846, 1983, 2070
AluI	AGCT	7 cut(s)	44, 376, 703, 975, 1846, 1983, 2070
Alw21I	GWGCWC	3 cut(s)	217, 1985, 2158
Alw26I	GTCTC	4 cut(s)	1698, 1739, 1914, 2097
AlwI	GGATC	7 cut(s)	477, 659, 672, 1205, 1486, 1888, 1896
Ama87I	CYCGRG	1 cut(s)	1984
AoxI	GGCC	3 cut(s)	726, 1112, 1574
ApeKI	GCWGC	2 cut(s)	1160, 2059
ApoI	RAATTY	4 cut(s)	275, 282, 1180, 2118
AscI	GGCGCGCC	1 cut(s)	1399
AspA2I	CCTAGG	1 cut(s)	1876
AspLEI	GCGC	4 cut(s)	691, 1008, 1401, 1403
AspS9I	GGNCC	4 cut(s)	1112, 1197, 1225, 2206
AsuC2I	CCSGG	1 cut(s)	1090
AsuII	TTCGAA	2 cut(s)	411, 1345
AsuNHI	GCTAGC	1 cut(s)	1094
AvaI	CYCGRG	1 cut(s)	1984
AvaII	GGWCC	3 cut(s)	1197, 1225, 2206
AvrII	CCTAGG	1 cut(s)	1876
BaeGI	GKGCMC	1 cut(s)	872
BalI	TGGCCA	1 cut(s)	1576
BamHI	GGATCC	1 cut(s)	664
BanII	GRGCYC	1 cut(s)	1985
BbsI	GAAGAC	1 cut(s)	574
Bbv12I	GWGCWC	3 cut(s)	217, 1985, 2158
BbvI	GCAGC	2 cut(s)	1147, 2071
BceAI	ACGGC	1 cut(s)	1108
BclI	TGATCA	1 cut(s)	766
BcnI	CCSGG	1 cut(s)	1090
BcoDI	GTCTC	4 cut(s)	1698, 1739, 1914, 2097
BfaI	CTAG	5 cut(s)	873, 989, 1095, 1626, 1877
BfmI	CTRYAG	1 cut(s)	927
BfoI	RGCGCY	1 cut(s)	1009
BglII	AGATCT	1 cut(s)	991
BisI	GCNGC	2 cut(s)	1161, 2060
BlnI	CCTAGG	1 cut(s)	1876
BlsI	GCNGC	2 cut(s)	1162, 2061
Bme1390I	CCNGG	1 cut(s)	1090
Bme18I	GGWCC	3 cut(s)	1197, 1225, 2206
BmeT110I	CYCGRG	1 cut(s)	1984
BmgT120I	GGNCC	4 cut(s)	1112, 1197, 1225, 2206
BmiI	GGNNCC	3 cut(s)	191, 666, 1227
BmrFI	CCNGG	1 cut(s)	1090
BmsI	GCATC	8 cut(s)	476, 502, 814, 1123, 1702, 1852, 1931, 2079
BmtI	GCTAGC	1 cut(s)	1098
BpiI	GAAGAC	1 cut(s)	574
Bpu14I	TTCGAA	2 cut(s)	411, 1345
BpuEI	CTTGAG	1 cut(s)	356
BpuMI	CCSGG	1 cut(s)	1090
BsaI	GGTCTC	2 cut(s)	1739, 2097
BsaJI	CCNNGG	8 cut(s)	28, 729, 1089, 1124, 1356, 1750, 1876, 1905
BsaWI	WCCGGW	2 cut(s)	378, 2106
BsaXI	ACNNNNNCTCC	2 cut(s)	360, 390
Bsc4I	CCNNNNNNNGG	6 cut(s)	422, 544, 600, 1284, 1357, 2113
Bse118I	RCCGGY	1 cut(s)	1151
Bse1I	ACTGG	3 cut(s)	948, 1429, 1948
Bse3DI	GCAATG	1 cut(s)	621
BseDI	CCNNGG	8 cut(s)	28, 729, 1089, 1124, 1356, 1750, 1876, 1905
BseGI	GGATG	7 cut(s)	217, 678, 769, 1174, 1484, 1946, 2115
BseLI	CCNNNNNNNGG	6 cut(s)	422, 544, 600, 1284, 1357, 2113
BseMI	GCAATG	1 cut(s)	621
BseMII	CTCAG	3 cut(s)	1272, 1581, 1917
BseNI	ACTGG	3 cut(s)	948, 1429, 1948
BsePI	GCGCGC	1 cut(s)	1399
BseRI	GAGGAG	2 cut(s)	1122, 1767
BseSI	GKGCMC	1 cut(s)	872
BseXI	GCAGC	2 cut(s)	1147, 2071
BsgI	GTGCAG	2 cut(s)	1625, 2102
Bsh1236I	CGCG	4 cut(s)	177, 685, 691, 1401
Bsh1285I	CGRYCG	1 cut(s)	181
BshFI	GGCC	3 cut(s)	728, 1114, 1576
BsiEI	CGRYCG	1 cut(s)	181
BsiHKAI	GWGCWC	3 cut(s)	217, 1985, 2158
BsiHKCI	CYCGRG	1 cut(s)	1984
BsiSI	CCGG	5 cut(s)	379, 545, 1089, 1152, 2107
BslFI	GGGAC	2 cut(s)	439, 1211
BslI	CCNNNNNNNGG	6 cut(s)	422, 544, 600, 1284, 1357, 2113
BsmAI	GTCTC	4 cut(s)	1698, 1739, 1914, 2097
BsmFI	GGGAC	2 cut(s)	439, 1211
BsmI	GAATGC	4 cut(s)	469, 1368, 1807, 2081
BsnI	GGCC	3 cut(s)	728, 1114, 1576
Bso31I	GGTCTC	2 cut(s)	1739, 2097
BsoBI	CYCGRG	1 cut(s)	1984
Bsp119I	TTCGAA	2 cut(s)	411, 1345
Bsp1286I	GDGCHC	4 cut(s)	217, 872, 1985, 2158
Bsp19I	CCATGG	2 cut(s)	28, 1905
BspACI	CCGC	4 cut(s)	221, 626, 683, 883
BspANI	GGCC	3 cut(s)	728, 1114, 1576
BspCNI	CTCAG	3 cut(s)	1273, 1582, 1918
BspFNI	CGCG	4 cut(s)	177, 685, 691, 1401
BspHI	TCATGA	3 cut(s)	969, 1372, 1848
BspLI	GGNNCC	3 cut(s)	191, 666, 1227
BspOI	GCTAGC	1 cut(s)	1098
BspPI	GGATC	7 cut(s)	477, 659, 672, 1205, 1486, 1888, 1896
BspT104I	TTCGAA	2 cut(s)	411, 1345
BspTNI	GGTCTC	2 cut(s)	1739, 2097
BsrDI	GCAATG	1 cut(s)	621
BsrFI	RCCGGY	1 cut(s)	1151
BsrI	ACTGG	3 cut(s)	948, 1429, 1948
BssAI	RCCGGY	1 cut(s)	1151
BssECI	CCNNGG	8 cut(s)	28, 729, 1089, 1124, 1356, 1750, 1876, 1905
BssHII	GCGCGC	1 cut(s)	1399
BssNAI	GTATAC	1 cut(s)	586
BssT1I	CCWWGG	4 cut(s)	28, 1750, 1876, 1905
Bst1107I	GTATAC	1 cut(s)	586
Bst4CI	ACNGT	8 cut(s)	182, 326, 436, 928, 1357, 1961, 2052, 2126
BstAPI	GCANNNNNTGC	2 cut(s)	867, 2089
BstBI	TTCGAA	2 cut(s)	411, 1345
BstC8I	GCNNGC	7 cut(s)	120, 628, 687, 1096, 1333, 1401, 2081
BstDEI	CTNAG	6 cut(s)	645, 1281, 1307, 1590, 1926, 2071
BstDSI	CCRYGG	4 cut(s)	28, 1124, 1356, 1905
BstEII	GGTNACC	1 cut(s)	1584
BstENI	CCTNNNNNAGG	1 cut(s)	1282
BstF5I	GGATG	7 cut(s)	217, 678, 769, 1174, 1484, 1946, 2115
BstFNI	CGCG	4 cut(s)	177, 685, 691, 1401
BstH2I	RGCGCY	1 cut(s)	1009
BstHHI	GCGC	4 cut(s)	691, 1008, 1401, 1403
BstMAI	GTCTC	4 cut(s)	1698, 1739, 1914, 2097
BstMCI	CGRYCG	1 cut(s)	181
BstMWI	GCNNNNNNNGC	5 cut(s)	867, 1120, 1989, 2076, 2089
BstPI	GGTNACC	1 cut(s)	1584
BstSCI	CCNGG	1 cut(s)	1088
BstSFI	CTRYAG	1 cut(s)	927
BstSLI	GKGCMC	1 cut(s)	872
BstUI	CGCG	4 cut(s)	177, 685, 691, 1401
BstV1I	GCAGC	2 cut(s)	1147, 2071
BstV2I	GAAGAC	1 cut(s)	574
BstX2I	RGATCY	4 cut(s)	664, 991, 1880, 1901
BstYI	RGATCY	4 cut(s)	664, 991, 1880, 1901
BstZ17I	GTATAC	1 cut(s)	586
BsuRI	GGCC	3 cut(s)	728, 1114, 1576
BtgI	CCRYGG	4 cut(s)	28, 1124, 1356, 1905
BtsCI	GGATG	7 cut(s)	217, 678, 769, 1174, 1484, 1946, 2115
BtsI	GCAGTG	4 cut(s)	520, 750, 1557, 2227
BtsIMutI	CAGTG	7 cut(s)	520, 750, 955, 1557, 1923, 2131, 2227
Cac8I	GCNNGC	7 cut(s)	120, 628, 687, 1096, 1333, 1401, 2081
CciI	TCATGA	3 cut(s)	969, 1372, 1848
CfoI	GCGC	4 cut(s)	691, 1008, 1401, 1403
Cfr10I	RCCGGY	1 cut(s)	1151
Cfr13I	GGNCC	4 cut(s)	1112, 1197, 1225, 2206
Csp6I	GTAC	2 cut(s)	341, 2023
CviQI	GTAC	2 cut(s)	341, 2023
DdeI	CTNAG	6 cut(s)	645, 1281, 1307, 1590, 1926, 2071
DraIII	CACNNNGTG	1 cut(s)	1061
EaeI	YGGCCR	1 cut(s)	1574
EciI	GGCGGA	1 cut(s)	210
Ecl136II	GAGCTC	1 cut(s)	1983
Eco130I	CCWWGG	4 cut(s)	28, 1750, 1876, 1905
Eco24I	GRGCYC	1 cut(s)	1985
Eco31I	GGTCTC	2 cut(s)	1739, 2097
Eco47I	GGWCC	3 cut(s)	1197, 1225, 2206
Eco53kI	GAGCTC	1 cut(s)	1983
Eco57I	CTGAAG	1 cut(s)	1085
Eco88I	CYCGRG	1 cut(s)	1984
Eco91I	GGTNACC	1 cut(s)	1584
EcoICRI	GAGCTC	1 cut(s)	1983
EcoNI	CCTNNNNNAGG	1 cut(s)	1282
EcoO109I	RGGNCCY	1 cut(s)	2206
EcoO65I	GGTNACC	1 cut(s)	1584
EcoT14I	CCWWGG	4 cut(s)	28, 1750, 1876, 1905
EcoT22I	ATGCAT	3 cut(s)	469, 829, 1867
EcoT38I	GRGCYC	1 cut(s)	1985
ErhI	CCWWGG	4 cut(s)	28, 1750, 1876, 1905
FaqI	GGGAC	2 cut(s)	439, 1211
FauI	CCCGC	2 cut(s)	619, 690
FauNDI	CATATG	1 cut(s)	124
FbaI	TGATCA	1 cut(s)	766
FblI	GTMKAC	3 cut(s)	585, 930, 2142
Fnu4HI	GCNGC	2 cut(s)	1161, 2060
FokI	GGATG	7 cut(s)	204, 665, 776, 1181, 1491, 1953, 2122
FriOI	GRGCYC	1 cut(s)	1985
Fsp4HI	GCNGC	2 cut(s)	1161, 2060
FspBI	CTAG	5 cut(s)	873, 989, 1095, 1626, 1877
GlaI	GCGC	4 cut(s)	690, 1007, 1400, 1402
GluI	GCNGC	2 cut(s)	1161, 2060
HaeII	RGCGCY	1 cut(s)	1009
HaeIII	GGCC	3 cut(s)	728, 1114, 1576
HapII	CCGG	5 cut(s)	379, 545, 1089, 1152, 2107
HhaI	GCGC	4 cut(s)	691, 1008, 1401, 1403
Hin6I	GCGC	4 cut(s)	689, 1006, 1399, 1401
HinP1I	GCGC	4 cut(s)	689, 1006, 1399, 1401
HincII	GTYRAC	1 cut(s)	2143
HindII	GTYRAC	1 cut(s)	2143
HindIII	AAGCTT	1 cut(s)	2068
HinfI	GANTC	5 cut(s)	227, 447, 559, 1052, 1564
HpaII	CCGG	5 cut(s)	379, 545, 1089, 1152, 2107
Hpy166II	GTNNAC	7 cut(s)	586, 931, 1274, 1598, 1837, 1858, 2143
Hpy188I	TCNGA	7 cut(s)	564, 915, 1104, 1282, 1954, 2102, 2188
Hpy8I	GTNNAC	7 cut(s)	586, 931, 1274, 1598, 1837, 1858, 2143
HpyCH4III	ACNGT	8 cut(s)	182, 326, 436, 928, 1357, 1961, 2052, 2126
HpyCH4IV	ACGT	1 cut(s)	1383
HpyF10VI	GCNNNNNNNGC	5 cut(s)	867, 1120, 1989, 2076, 2089
HpyF3I	CTNAG	6 cut(s)	645, 1281, 1307, 1590, 1926, 2071
HpySE526I	ACGT	1 cut(s)	1383
HspAI	GCGC	4 cut(s)	689, 1006, 1399, 1401
Ksp22I	TGATCA	1 cut(s)	766
LmnI	GCTCC	4 cut(s)	266, 381, 1018, 2000
Lsp1109I	GCAGC	2 cut(s)	1147, 2071
LweI	GCATC	8 cut(s)	476, 502, 814, 1123, 1702, 1852, 1931, 2079
MaeI	CTAG	5 cut(s)	873, 989, 1095, 1626, 1877
MaeII	ACGT	1 cut(s)	1383
MaeIII	GTNAC	7 cut(s)	127, 496, 833, 956, 1584, 1961, 2164
MboII	GAAGA	8 cut(s)	43, 579, 590, 833, 892, 2057, 2098, 2144
MfeI	CAATTG	3 cut(s)	94, 1314, 1337
MflI	RGATCY	4 cut(s)	664, 991, 1880, 1901
MhlI	GDGCHC	4 cut(s)	217, 872, 1985, 2158
MlsI	TGGCCA	1 cut(s)	1576
MluNI	TGGCCA	1 cut(s)	1576
MlyI	GAGTC	1 cut(s)	568
MmeI	TCCRAC	2 cut(s)	510, 1977
Mox20I	TGGCCA	1 cut(s)	1576
Mph1103I	ATGCAT	3 cut(s)	469, 829, 1867
MscI	TGGCCA	1 cut(s)	1576
MseI	TTAA	4 cut(s)	396, 438, 779, 1241
MslI	CAYNNNNRTG	3 cut(s)	245, 851, 1853
Msp20I	TGGCCA	1 cut(s)	1576
MspI	CCGG	5 cut(s)	379, 545, 1089, 1152, 2107
MspR9I	CCNGG	1 cut(s)	1090
MunI	CAATTG	3 cut(s)	94, 1314, 1337
Mva1269I	GAATGC	4 cut(s)	469, 1368, 1807, 2081
MvnI	CGCG	4 cut(s)	177, 685, 691, 1401
MwoI	GCNNNNNNNGC	5 cut(s)	867, 1120, 1989, 2076, 2089
NciI	CCSGG	1 cut(s)	1090
NcoI	CCATGG	2 cut(s)	28, 1905
NdeI	CATATG	1 cut(s)	124
NheI	GCTAGC	1 cut(s)	1094
NlaIV	GGNNCC	3 cut(s)	191, 666, 1227
NmeAIII	GCCGAG	1 cut(s)	754
NmuCI	GTSAC	5 cut(s)	127, 496, 956, 1961, 2164
NsiI	ATGCAT	3 cut(s)	469, 829, 1867
NspV	TTCGAA	2 cut(s)	411, 1345
OliI	CACNNNNGTG	1 cut(s)	851
PagI	TCATGA	3 cut(s)	969, 1372, 1848
PalAI	GGCGCGCC	1 cut(s)	1399
PauI	GCGCGC	1 cut(s)	1399
PctI	GAATGC	4 cut(s)	469, 1368, 1807, 2081
PfeI	GAWTC	4 cut(s)	227, 447, 1052, 1564
PflFI	GACNNNGTC	1 cut(s)	1636
PkrI	GCNGC	2 cut(s)	1162, 2061
PleI	GAGTC	1 cut(s)	567
PpsI	GAGTC	1 cut(s)	567
PpuMI	RGGWCCY	1 cut(s)	2206
Psp124BI	GAGCTC	1 cut(s)	1985
Psp5II	RGGWCCY	1 cut(s)	2206
PspEI	GGTNACC	1 cut(s)	1584
PspN4I	GGNNCC	3 cut(s)	191, 666, 1227
PspPI	GGNCC	4 cut(s)	1112, 1197, 1225, 2206
PspPPI	RGGWCCY	1 cut(s)	2206
PsuI	RGATCY	4 cut(s)	664, 991, 1880, 1901
PsyI	GACNNNGTC	1 cut(s)	1636
PteI	GCGCGC	1 cut(s)	1399
RsaI	GTAC	2 cut(s)	342, 2024
RsaNI	GTAC	2 cut(s)	341, 2023
RseI	CAYNNNNRTG	3 cut(s)	245, 851, 1853
SacI	GAGCTC	1 cut(s)	1985
SalI	GTCGAC	1 cut(s)	2141
SaqAI	TTAA	4 cut(s)	396, 438, 779, 1241
SatI	GCNGC	2 cut(s)	1161, 2060
Sau96I	GGNCC	4 cut(s)	1112, 1197, 1225, 2206
SchI	GAGTC	1 cut(s)	568
ScrFI	CCNGG	1 cut(s)	1090
SduI	GDGCHC	4 cut(s)	217, 872, 1985, 2158
SfaNI	GCATC	8 cut(s)	476, 502, 814, 1123, 1702, 1852, 1931, 2079
SfcI	CTRYAG	1 cut(s)	927
SfuI	TTCGAA	2 cut(s)	411, 1345
SgsI	GGCGCGCC	1 cut(s)	1399
SinI	GGWCC	3 cut(s)	1197, 1225, 2206
SmiMI	CAYNNNNRTG	3 cut(s)	245, 851, 1853
SmlI	CTYRAG	1 cut(s)	371
SmoI	CTYRAG	1 cut(s)	371
SsiI	CCGC	4 cut(s)	221, 626, 683, 883
SspI	AATATT	3 cut(s)	84, 1796, 2289
SspMI	CTAG	5 cut(s)	873, 989, 1095, 1626, 1877
SstI	GAGCTC	1 cut(s)	1985
StyD4I	CCNGG	1 cut(s)	1088
StyI	CCWWGG	4 cut(s)	28, 1750, 1876, 1905
TaaI	ACNGT	8 cut(s)	182, 326, 436, 928, 1357, 1961, 2052, 2126
TaiI	ACGT	1 cut(s)	1386
TaqI	TCGA	8 cut(s)	230, 345, 411, 1185, 1345, 1422, 1918, 2142
TatI	WGTACW	1 cut(s)	340
TfiI	GAWTC	4 cut(s)	227, 447, 1052, 1564
Tru1I	TTAA	4 cut(s)	396, 438, 779, 1241
Tru9I	TTAA	4 cut(s)	396, 438, 779, 1241
TscAI	CASTG	7 cut(s)	527, 750, 955, 1557, 1930, 2131, 2227
TseFI	GTSAC	5 cut(s)	127, 496, 956, 1961, 2164
TseI	GCWGC	2 cut(s)	1160, 2059
Tsp45I	GTSAC	5 cut(s)	127, 496, 956, 1961, 2164
TspDTI	ATGAA	8 cut(s)	263, 765, 834, 1361, 1389, 1426, 1607, 1813
TspGWI	ACGGA	3 cut(s)	126, 1031, 1926
TspRI	CASTG	7 cut(s)	527, 750, 955, 1557, 1930, 2131, 2227
Tth111I	GACNNNGTC	1 cut(s)	1636
VpaK11BI	GGWCC	3 cut(s)	1197, 1225, 2206
XagI	CCTNNNNNAGG	1 cut(s)	1282
XapI	RAATTY	4 cut(s)	275, 282, 1180, 2118
XbaI	TCTAGA	1 cut(s)	988
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1122
XmaJI	CCTAGG	1 cut(s)	1876
XmiI	GTMKAC	3 cut(s)	585, 930, 2142
XspI	CTAG	5 cut(s)	873, 989, 1095, 1626, 1877
Zsp2I	ATGCAT	3 cut(s)	469, 829, 1867

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.