Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0009530

Physical Location & Seq

Genomic Coordinates Reverse (-)

19628165 .. 19631303

Gene Structure 3139 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1175200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2298 bp

ATGGAGGGAAAAGTACATAAGTTTCTCCATGGAACTCTTGAAGCTAACATCTTCCATGCCACACCTTACTCACCACCACCATTCCCCTTCAATTGTATTTTTGCCACTGGGAAGCCTGCATATGTGACCATCAAAATAGACAACAAGAAAGTAGCAAAGACGACCCATGAACGAGACCGTGTGTGGAACCAAACCTTTCAAATCCCTTGTGCTTATCCGCCTGATTCGACCATTACCATTACCATGAAAACAAAGTGCTCCATCTTGGGAAAATTTCAAATTCAGGCACATGAGATACTAAACGAAGCAAGTTTTATCAACGGTTTCCTCCCTCTTGTAATCGAAAATGGGAAGCCAAATCCAGAACTCAAGCTCCGGTTCATGTTATGGTTTAAGCCTGTGCAGTTCGAACCAACTTGGGGGACAATAACTGATAATGGTGGATTCAAGGGGCTGAGGAATGCATCATTTCCACAAAGATCCAACAGCCATGTCACTCTTTATCAAGATGCTCACCATTGCTCAACTTTCAACCCTACGCCGGAGTTCTGTGGGACTCCGAGAAGACTATGGGAAAATGTTTACACAGCCATTGAGGGGGCAACACATTTGATTTACATTGCGGGCTGGTCTTTCAATCCTAAAATGGTTCTGGTCAGGGATCTTCAAACAGACAACCTGCGTGCGCGAGGGGTGAAGCTCGGTGAATTACTAAAGCAGAAGGCTGAGGAAGGTGTGGCAGTGAGAGTAATGCTTTGGAATGATGAAACTTCTTTGAAAATCATCAAGAACAAAGGAATAATGGGAACACATGATGAAGATGCATTTTGTTACTTTGCACACACCAAAGTGATATGCAAATTGTGCCCTAGATTGCACCACAAGTTTCCCACAATCTTCTCTCACCATCAGAAAACCATAACTGTAGACATCAAATCTAGCACCAGTGCAAGTGACAGAGAAATCATGAGCTTTGTTGGGGGTCTAGATCTTTGCGATGGGCGCTACGACACAGAGCAACATTCGTTGTTTCAAACCCTCAATACAGAATCACATTGTGCTGATTTCTACCAGACCAACATTTCCGGGGCTAGCCTTCAGAGAGGAGGGCCTAGAACGCCGTGGCATGATGCTCACGCTTGTATAACCGGTGAGGCTGCTTGGGATGTACTAACAAATTTCGAGCAACGATGGACTAAGCAATGTGATCCCTCTTTATTGGTCCCTTCTATCACCTTAACAAGCCTCTTTCAACAGACTTATGCAAGTAAACCCTCTGAGAGGGGCTGGAATGTTCAAGTTCTTAGGTCAATTGACAATGTTTCAGCAGGCCAATTGTTTCAAAACCTAACCGTGGAGCATAGCATTCACGACGCCTACGTTGAAGCAATTAGGAGCGCCGATAAGTTTCTATACATCGAGAACCAGTATTTCATGGGAGGGTGCTATCTGTGGGAGAACGATCAGCATTGTGGATGTAGAAACTTGATCCCCGTTGAAATTGCACTAAAAGTGGTCCATAAGATCAAAGCAAAGGAGAGGTTTGCAGTGTATATTGTGATTCCGATGTGGCCAGAAGGGTTACCTGAGAGTGAATCTGTGCAGGATATACTTCATTGGACTAGAGAGACGATGTCAATGATGTATAAATTGGTTGGAGAAGCCATAAAAGAAAGTGGGGTTGAAGGGCATCACCCGAGAGACTATTTGAACTTCTATTGCCTTGCCAATAGAGAAGTTGAGATCCAAGGGGAGTTTGTTCCCCCTCAATCTCCTTATCATGCAACACAATATTGGAATGCACAAAAGCATAGGAGGTTCATGGTCTATGTTCACTCCAAGCTCATGATAGTGGACGATGCATACCTTATCATAGGATCAGCAAACATAAACCAAAGATCCATGGATGGACACCGAGACACTGAGATCGCAATTGGATGCTACCAGTCCGACAATGGCGATAACAAGACGATGGGGAGCTCGAGAGGCGACATTGGAGCATACCGGATGTCATTATGGTATGAACACACAGGTCGTGCCAAAGAATTGTTCAAGCATCCTGAAAGCTTGGAATGCGTGCAGACGATGCGTTTTATCGGAGATCGGATGTGGAAAATTTACAGTGGAGAAGAAGTGATCGACATGGAAGGTGTTCATCTTGTGACATACCCCATAAATATAACAGAGGAAGGATATGTTGAGGACCTTGTGGAAGGTAGTGGTGATCATTTTCCAGATACAAATTCTATGGTGAAGGGCAAGAGATCAAAAGTGATCCCACCAATATTTACTACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004620 GO:0004630 GO:0005575 GO:0005623 GO:0005886 GO:0006629 GO:0006644 GO:0006793 GO:0006796 GO:0006808 GO:0006950 GO:0006970 GO:0006995 GO:0007154 GO:0007275 GO:0008081 GO:0008150 GO:0008152 GO:0009056 GO:0009267 GO:0009395 GO:0009605 GO:0009628 GO:0009791 GO:0009825 GO:0009987 GO:0009991 GO:0016020 GO:0016036 GO:0016042 GO:0016049 GO:0016298 GO:0016787 GO:0016788 GO:0019637 GO:0022622 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0033554 GO:0040007 GO:0042578 GO:0042594 GO:0043562 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044464 GO:0045848 GO:0046434 GO:0048364 GO:0048518 GO:0048731 GO:0048856 GO:0050789 GO:0050896 GO:0051301 GO:0051365 GO:0051716 GO:0065007 GO:0071496 GO:0071704 GO:0071944 GO:0099402 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

C2 PLD_C PLDc

Protein Analysis

766

Amino Acids

87.3

Weight (kDa)

6.51

Isoelectric Point (pI)

36.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PLDc_2	PF13091	607 - 645	2.2e-06	PLD-like domain
PLDc	PF00614	611 - 634	1.2e-06	Phospholipase D Active site motif
PLD_C	PF12357	682 - 754	3.1e-22	Phospholipase D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009530)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55180 AT1G55180
fragaria_vesca	FvH4_6g33140
malus_domestica	MD09G1194700.v1.1 MD17G1175200.v1.1
prunus_persica	Prupe.3G051200_v2.0.a1
pyrus_communis	pycom09g11160
rosa_chinensis	RchiOBHm_Chr2g0142831
rosa_laevigata	RLG00000019989
rosa_multiflora	Rmu_co8241083.1_g000001 Rmu_co8446503.1_g000001 Rmu_sc0012306.1_g000009 Rmu_sc0013116.1_g000004
rosa_roxburghii	Rroxscaffold_2G00102280 Rroxscaffold_2G00103320
rosa_rugosa	Rorug02G0375600 Rorug02G0375700
rosa_samantha	Rh2AG428800 Rh2BG438100 Rh2CG414300 Rh2DG447900
rosa_wichuraiana	Rw2G035060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	687
AccI	GTMKAC	1 cut(s)	927
AccII	CGCG	1 cut(s)	688
AciI	CCGC	2 cut(s)	218, 623
AclWI	GGATC	8 cut(s)	474, 669, 1202, 1483, 1738, 1885, 1893, 2269
AcoI	YGGCCR	1 cut(s)	1571
AcsI	RAATTY	5 cut(s)	272, 279, 1177, 2115, 2242
AcuI	CTGAAG	1 cut(s)	1082
AcyI	GRCGYC	1 cut(s)	1374
AdeI	CACNNNGTG	1 cut(s)	1058
AfaI	GTAC	2 cut(s)	15, 1170
AfiI	CCNNNNNNNGG	5 cut(s)	419, 541, 597, 1281, 1354
AgeI	ACCGGT	1 cut(s)	1148
AjuI	GAANNNNNNNTTGG	2 cut(s)	2033, 2065
AleI	CACNNNNGTG	1 cut(s)	848
AloI	GAACNNNNNNTCC	2 cut(s)	357, 389
AluBI	AGCT	7 cut(s)	44, 373, 700, 972, 1843, 1980, 2067
AluI	AGCT	7 cut(s)	44, 373, 700, 972, 1843, 1980, 2067
Alw21I	GWGCWC	2 cut(s)	260, 1982
Alw26I	GTCTC	4 cut(s)	168, 1622, 1695, 1911
AlwI	GGATC	8 cut(s)	474, 669, 1202, 1483, 1738, 1885, 1893, 2269
Ama87I	CYCGRG	2 cut(s)	1696, 1981
AoxI	GGCC	3 cut(s)	1109, 1330, 1571
ApeKI	GCWGC	1 cut(s)	1157
ApoI	RAATTY	5 cut(s)	272, 279, 1177, 2115, 2242
AsiGI	ACCGGT	1 cut(s)	1148
Asp700I	GAANNNNTTC	1 cut(s)	2151
AspLEI	GCGC	3 cut(s)	688, 1005, 1400
AspS9I	GGNCC	4 cut(s)	1109, 1222, 1516, 2203
AsuC2I	CCSGG	1 cut(s)	1087
AsuII	TTCGAA	1 cut(s)	408
AsuNHI	GCTAGC	1 cut(s)	1091
AvaI	CYCGRG	2 cut(s)	1696, 1981
AvaII	GGWCC	3 cut(s)	1222, 1516, 2203
BaeGI	GKGCMC	1 cut(s)	869
BalI	TGGCCA	1 cut(s)	1573
BanII	GRGCYC	1 cut(s)	1982
BbsI	GAAGAC	1 cut(s)	571
Bbv12I	GWGCWC	2 cut(s)	260, 1982
BbvCI	CCTCAGC	2 cut(s)	455, 726
BbvI	GCAGC	1 cut(s)	1144
BccI	CCATC	7 cut(s)	137, 269, 915, 992, 1185, 1901, 1966
BceAI	ACGGC	1 cut(s)	1105
BclI	TGATCA	1 cut(s)	2224
BcnI	CCSGG	1 cut(s)	1087
BcoDI	GTCTC	4 cut(s)	168, 1622, 1695, 1911
BfaI	CTAG	6 cut(s)	870, 939, 986, 1092, 1113, 1623
BfmI	CTRYAG	1 cut(s)	924
BfoI	RGCGCY	2 cut(s)	1006, 1401
BfuAI	ACCTGC	1 cut(s)	687
BglII	AGATCT	1 cut(s)	988
BisI	GCNGC	1 cut(s)	1158
BlsI	GCNGC	1 cut(s)	1159
Bme1390I	CCNGG	1 cut(s)	1087
Bme18I	GGWCC	3 cut(s)	1222, 1516, 2203
BmeT110I	CYCGRG	2 cut(s)	1696, 1981
BmgT120I	GGNCC	4 cut(s)	1109, 1222, 1516, 2203
BmiI	GGNNCC	2 cut(s)	188, 1224
BmrFI	CCNGG	1 cut(s)	1087
BmrI	ACTGGG	1 cut(s)	117
BmsI	GCATC	9 cut(s)	473, 499, 811, 1120, 1699, 1849, 1928, 2065, 2076
BmtI	GCTAGC	1 cut(s)	1095
BmuI	ACTGGG	1 cut(s)	117
BpiI	GAAGAC	1 cut(s)	571
Bpu10I	CCTNAGC	2 cut(s)	455, 726
Bpu14I	TTCGAA	1 cut(s)	408
BpuEI	CTTGAG	1 cut(s)	353
BpuMI	CCSGG	1 cut(s)	1087
BsaHI	GRCGYC	1 cut(s)	1374
BsaI	GGTCTC	1 cut(s)	168
BsaJI	CCNNGG	6 cut(s)	28, 1086, 1121, 1353, 1747, 1902
BsaWI	WCCGGW	3 cut(s)	375, 1148, 2004
BsaXI	ACNNNNNCTCC	4 cut(s)	357, 387, 2118, 2148
Bsc4I	CCNNNNNNNGG	5 cut(s)	419, 541, 597, 1281, 1354
Bse118I	RCCGGY	1 cut(s)	1148
Bse1I	ACTGG	4 cut(s)	112, 945, 1426, 1945
Bse3DI	GCAATG	3 cut(s)	517, 618, 1208
BseDI	CCNNGG	6 cut(s)	28, 1086, 1121, 1353, 1747, 1902
BseGI	GGATG	7 cut(s)	1171, 1481, 1912, 1943, 2013, 2056, 2112
BseLI	CCNNNNNNNGG	5 cut(s)	419, 541, 597, 1281, 1354
BseMI	GCAATG	3 cut(s)	517, 618, 1208
BseMII	CTCAG	5 cut(s)	446, 717, 1269, 1578, 1914
BseNI	ACTGG	4 cut(s)	112, 945, 1426, 1945
BseRI	GAGGAG	1 cut(s)	1119
BseSI	GKGCMC	1 cut(s)	869
BseXI	GCAGC	1 cut(s)	1144
BsgI	GTGCAG	3 cut(s)	422, 1622, 2099
Bsh1236I	CGCG	1 cut(s)	688
BshFI	GGCC	3 cut(s)	1111, 1332, 1573
BshTI	ACCGGT	1 cut(s)	1148
BsiHKAI	GWGCWC	2 cut(s)	260, 1982
BsiHKCI	CYCGRG	2 cut(s)	1696, 1981
BsiSI	CCGG	5 cut(s)	376, 542, 1086, 1149, 2005
BslFI	GGGAC	3 cut(s)	436, 568, 1208
BslI	CCNNNNNNNGG	5 cut(s)	419, 541, 597, 1281, 1354
BsmAI	GTCTC	4 cut(s)	168, 1622, 1695, 1911
BsmBI	CGTCTC	1 cut(s)	1622
BsmFI	GGGAC	3 cut(s)	436, 568, 1208
BsmI	GAATGC	4 cut(s)	466, 1365, 1804, 2078
BsnI	GGCC	3 cut(s)	1111, 1332, 1573
Bso31I	GGTCTC	1 cut(s)	168
BsoBI	CYCGRG	2 cut(s)	1696, 1981
Bsp119I	TTCGAA	1 cut(s)	408
Bsp1286I	GDGCHC	3 cut(s)	260, 869, 1982
Bsp19I	CCATGG	2 cut(s)	28, 1902
BspACI	CCGC	2 cut(s)	218, 623
BspANI	GGCC	3 cut(s)	1111, 1332, 1573
BspCNI	CTCAG	5 cut(s)	447, 718, 1270, 1579, 1915
BspFNI	CGCG	1 cut(s)	688
BspHI	TCATGA	2 cut(s)	966, 1845
BspLI	GGNNCC	2 cut(s)	188, 1224
BspMI	ACCTGC	1 cut(s)	687
BspOI	GCTAGC	1 cut(s)	1095
BspPI	GGATC	8 cut(s)	474, 669, 1202, 1483, 1738, 1885, 1893, 2269
BspT104I	TTCGAA	1 cut(s)	408
BspTNI	GGTCTC	1 cut(s)	168
BsrDI	GCAATG	3 cut(s)	517, 618, 1208
BsrFI	RCCGGY	1 cut(s)	1148
BsrI	ACTGG	4 cut(s)	112, 945, 1426, 1945
BssAI	RCCGGY	1 cut(s)	1148
BssECI	CCNNGG	6 cut(s)	28, 1086, 1121, 1353, 1747, 1902
BssNI	GRCGYC	1 cut(s)	1374
BssT1I	CCWWGG	3 cut(s)	28, 1747, 1902
Bst4CI	ACNGT	5 cut(s)	179, 323, 925, 1354, 2123
BstACI	GRCGYC	1 cut(s)	1374
BstAPI	GCANNNNNTGC	2 cut(s)	864, 2086
BstBI	TTCGAA	1 cut(s)	408
BstC8I	GCNNGC	6 cut(s)	117, 625, 684, 1093, 1330, 2078
BstDEI	CTNAG	7 cut(s)	455, 726, 1197, 1278, 1304, 1587, 1923
BstDSI	CCRYGG	4 cut(s)	28, 1121, 1353, 1902
BstEII	GGTNACC	1 cut(s)	1581
BstENI	CCTNNNNNAGG	1 cut(s)	1279
BstF5I	GGATG	7 cut(s)	1171, 1481, 1912, 1943, 2013, 2056, 2112
BstFNI	CGCG	1 cut(s)	688
BstH2I	RGCGCY	2 cut(s)	1006, 1401
BstHHI	GCGC	3 cut(s)	688, 1005, 1400
BstMAI	GTCTC	4 cut(s)	168, 1622, 1695, 1911
BstMWI	GCNNNNNNNGC	6 cut(s)	864, 1002, 1117, 1986, 2073, 2086
BstPI	GGTNACC	1 cut(s)	1581
BstSCI	CCNGG	1 cut(s)	1085
BstSFI	CTRYAG	1 cut(s)	924
BstSLI	GKGCMC	1 cut(s)	869
BstUI	CGCG	1 cut(s)	688
BstV1I	GCAGC	1 cut(s)	1144
BstV2I	GAAGAC	1 cut(s)	571
BstX2I	RGATCY	5 cut(s)	479, 661, 988, 1743, 1898
BstYI	RGATCY	5 cut(s)	479, 661, 988, 1743, 1898
BsuRI	GGCC	3 cut(s)	1111, 1332, 1573
BtgI	CCRYGG	4 cut(s)	28, 1121, 1353, 1902
BtgZI	GCGATG	1 cut(s)	1011
BtsCI	GGATG	7 cut(s)	1171, 1481, 1912, 1943, 2013, 2056, 2112
BtsI	GCAGTG	2 cut(s)	747, 1554
BtsIMutI	CAGTG	6 cut(s)	105, 747, 952, 1554, 1920, 2128
BveI	ACCTGC	1 cut(s)	687
Cac8I	GCNNGC	6 cut(s)	117, 625, 684, 1093, 1330, 2078
CciI	TCATGA	2 cut(s)	966, 1845
CfoI	GCGC	3 cut(s)	688, 1005, 1400
Cfr10I	RCCGGY	1 cut(s)	1148
Cfr13I	GGNCC	4 cut(s)	1109, 1222, 1516, 2203
CseI	GACGC	1 cut(s)	1382
Csp6I	GTAC	2 cut(s)	14, 1169
CspAI	ACCGGT	1 cut(s)	1148
CviQI	GTAC	2 cut(s)	14, 1169
DdeI	CTNAG	7 cut(s)	455, 726, 1197, 1278, 1304, 1587, 1923
DraIII	CACNNNGTG	1 cut(s)	1058
EaeI	YGGCCR	1 cut(s)	1571
EciI	GGCGGA	1 cut(s)	207
Ecl136II	GAGCTC	1 cut(s)	1980
Eco130I	CCWWGG	3 cut(s)	28, 1747, 1902
Eco24I	GRGCYC	1 cut(s)	1982
Eco31I	GGTCTC	1 cut(s)	168
Eco47I	GGWCC	3 cut(s)	1222, 1516, 2203
Eco53kI	GAGCTC	1 cut(s)	1980
Eco57I	CTGAAG	1 cut(s)	1082
Eco88I	CYCGRG	2 cut(s)	1696, 1981
Eco91I	GGTNACC	1 cut(s)	1581
EcoICRI	GAGCTC	1 cut(s)	1980
EcoNI	CCTNNNNNAGG	1 cut(s)	1279
EcoO109I	RGGNCCY	2 cut(s)	1109, 2203
EcoO65I	GGTNACC	1 cut(s)	1581
EcoT14I	CCWWGG	3 cut(s)	28, 1747, 1902
EcoT22I	ATGCAT	3 cut(s)	466, 826, 1864
EcoT38I	GRGCYC	1 cut(s)	1982
ErhI	CCWWGG	3 cut(s)	28, 1747, 1902
Esp3I	CGTCTC	1 cut(s)	1622
FaqI	GGGAC	3 cut(s)	436, 568, 1208
FauI	CCCGC	1 cut(s)	616
FauNDI	CATATG	1 cut(s)	121
FbaI	TGATCA	1 cut(s)	2224
FblI	GTMKAC	1 cut(s)	927
Fnu4HI	GCNGC	1 cut(s)	1158
FokI	GGATG	7 cut(s)	1178, 1488, 1919, 1950, 2020, 2043, 2119
FriOI	GRGCYC	1 cut(s)	1982
Fsp4HI	GCNGC	1 cut(s)	1158
FspBI	CTAG	6 cut(s)	870, 939, 986, 1092, 1113, 1623
GlaI	GCGC	3 cut(s)	687, 1004, 1399
GluI	GCNGC	1 cut(s)	1158
HaeII	RGCGCY	2 cut(s)	1006, 1401
HaeIII	GGCC	3 cut(s)	1111, 1332, 1573
HapII	CCGG	5 cut(s)	376, 542, 1086, 1149, 2005
HgaI	GACGC	1 cut(s)	1382
HhaI	GCGC	3 cut(s)	688, 1005, 1400
Hin1I	GRCGYC	1 cut(s)	1374
Hin6I	GCGC	3 cut(s)	686, 1003, 1398
HinP1I	GCGC	3 cut(s)	686, 1003, 1398
HindIII	AAGCTT	1 cut(s)	2065
HinfI	GANTC	6 cut(s)	224, 444, 556, 1049, 1561, 1595
HpaII	CCGG	5 cut(s)	376, 542, 1086, 1149, 2005
Hpy166II	GTNNAC	5 cut(s)	583, 928, 1271, 1834, 1855
Hpy188I	TCNGA	8 cut(s)	561, 912, 1101, 1279, 1566, 1951, 2099, 2106
Hpy8I	GTNNAC	5 cut(s)	583, 928, 1271, 1834, 1855
Hpy99I	CGWCG	1 cut(s)	1376
HpyCH4III	ACNGT	5 cut(s)	179, 323, 925, 1354, 2123
HpyCH4IV	ACGT	1 cut(s)	1380
HpyF10VI	GCNNNNNNNGC	6 cut(s)	864, 1002, 1117, 1986, 2073, 2086
HpyF3I	CTNAG	7 cut(s)	455, 726, 1197, 1278, 1304, 1587, 1923
HpySE526I	ACGT	1 cut(s)	1380
Hsp92I	GRCGYC	1 cut(s)	1374
HspAI	GCGC	3 cut(s)	686, 1003, 1398
Ksp22I	TGATCA	1 cut(s)	2224
LmnI	GCTCC	6 cut(s)	263, 378, 1357, 1395, 1977, 1997
Lsp1109I	GCAGC	1 cut(s)	1144
LweI	GCATC	9 cut(s)	473, 499, 811, 1120, 1699, 1849, 1928, 2065, 2076
MaeI	CTAG	6 cut(s)	870, 939, 986, 1092, 1113, 1623
MaeII	ACGT	1 cut(s)	1380
MaeIII	GTNAC	6 cut(s)	124, 493, 830, 953, 1581, 2161
MboII	GAAGA	6 cut(s)	43, 576, 656, 830, 889, 2141
MfeI	CAATTG	4 cut(s)	91, 1311, 1334, 1932
MflI	RGATCY	5 cut(s)	479, 661, 988, 1743, 1898
MhlI	GDGCHC	3 cut(s)	260, 869, 1982
MlsI	TGGCCA	1 cut(s)	1573
MluNI	TGGCCA	1 cut(s)	1573
MlyI	GAGTC	1 cut(s)	550
MmeI	TCCRAC	3 cut(s)	507, 1636, 1974
Mox20I	TGGCCA	1 cut(s)	1573
Mph1103I	ATGCAT	3 cut(s)	466, 826, 1864
MroXI	GAANNNNTTC	1 cut(s)	2151
MscI	TGGCCA	1 cut(s)	1573
MseI	TTAA	2 cut(s)	393, 1238
MslI	CAYNNNNRTG	3 cut(s)	242, 848, 1850
Msp20I	TGGCCA	1 cut(s)	1573
MspI	CCGG	5 cut(s)	376, 542, 1086, 1149, 2005
MspR9I	CCNGG	1 cut(s)	1087
MunI	CAATTG	4 cut(s)	91, 1311, 1334, 1932
Mva1269I	GAATGC	4 cut(s)	466, 1365, 1804, 2078
MvnI	CGCG	1 cut(s)	688
MwoI	GCNNNNNNNGC	6 cut(s)	864, 1002, 1117, 1986, 2073, 2086
NciI	CCSGG	1 cut(s)	1087
NcoI	CCATGG	2 cut(s)	28, 1902
NdeI	CATATG	1 cut(s)	121
NheI	GCTAGC	1 cut(s)	1091
NlaIV	GGNNCC	2 cut(s)	188, 1224
NmuCI	GTSAC	4 cut(s)	124, 493, 953, 2161
NsiI	ATGCAT	3 cut(s)	466, 826, 1864
NspV	TTCGAA	1 cut(s)	408
OliI	CACNNNNGTG	1 cut(s)	848
PaeR7I	CTCGAG	1 cut(s)	1981
PagI	TCATGA	2 cut(s)	966, 1845
PcsI	WCGNNNNNNNCGW	1 cut(s)	1377
PctI	GAATGC	4 cut(s)	466, 1365, 1804, 2078
PdmI	GAANNNNTTC	1 cut(s)	2151
PfeI	GAWTC	5 cut(s)	224, 444, 1049, 1561, 1595
PflFI	GACNNNGTC	1 cut(s)	1633
PinAI	ACCGGT	1 cut(s)	1148
PkrI	GCNGC	1 cut(s)	1159
PleI	GAGTC	1 cut(s)	550
PpsI	GAGTC	1 cut(s)	550
PpuMI	RGGWCCY	1 cut(s)	2203
Psp124BI	GAGCTC	1 cut(s)	1982
Psp5II	RGGWCCY	1 cut(s)	2203
PspEI	GGTNACC	1 cut(s)	1581
PspN4I	GGNNCC	2 cut(s)	188, 1224
PspPI	GGNCC	4 cut(s)	1109, 1222, 1516, 2203
PspPPI	RGGWCCY	1 cut(s)	2203
PsuI	RGATCY	5 cut(s)	479, 661, 988, 1743, 1898
PsyI	GACNNNGTC	1 cut(s)	1633
RsaI	GTAC	2 cut(s)	15, 1170
RsaNI	GTAC	2 cut(s)	14, 1169
RseI	CAYNNNNRTG	3 cut(s)	242, 848, 1850
SacI	GAGCTC	1 cut(s)	1982
SaqAI	TTAA	2 cut(s)	393, 1238
SatI	GCNGC	1 cut(s)	1158
Sau96I	GGNCC	4 cut(s)	1109, 1222, 1516, 2203
SchI	GAGTC	1 cut(s)	550
ScrFI	CCNGG	1 cut(s)	1087
SduI	GDGCHC	3 cut(s)	260, 869, 1982
SfaNI	GCATC	9 cut(s)	473, 499, 811, 1120, 1699, 1849, 1928, 2065, 2076
SfcI	CTRYAG	1 cut(s)	924
Sfr274I	CTCGAG	1 cut(s)	1981
SfuI	TTCGAA	1 cut(s)	408
SinI	GGWCC	3 cut(s)	1222, 1516, 2203
SlaI	CTCGAG	1 cut(s)	1981
SmiMI	CAYNNNNRTG	3 cut(s)	242, 848, 1850
SmlI	CTYRAG	2 cut(s)	368, 1981
SmoI	CTYRAG	2 cut(s)	368, 1981
SsiI	CCGC	2 cut(s)	218, 623
SspI	AATATT	2 cut(s)	1793, 2286
SspMI	CTAG	6 cut(s)	870, 939, 986, 1092, 1113, 1623
SstI	GAGCTC	1 cut(s)	1982
StyD4I	CCNGG	1 cut(s)	1085
StyI	CCWWGG	3 cut(s)	28, 1747, 1902
TaaI	ACNGT	5 cut(s)	179, 323, 925, 1354, 2123
TaiI	ACGT	1 cut(s)	1383
TaqI	TCGA	7 cut(s)	227, 342, 408, 1182, 1419, 1982, 2139
TatI	WGTACW	2 cut(s)	13, 1168
TfiI	GAWTC	5 cut(s)	224, 444, 1049, 1561, 1595
Tru1I	TTAA	2 cut(s)	393, 1238
Tru9I	TTAA	2 cut(s)	393, 1238
TscAI	CASTG	6 cut(s)	112, 747, 952, 1554, 1927, 2128
TseFI	GTSAC	4 cut(s)	124, 493, 953, 2161
TseI	GCWGC	1 cut(s)	1157
Tsp45I	GTSAC	4 cut(s)	124, 493, 953, 2161
TspRI	CASTG	6 cut(s)	112, 747, 952, 1554, 1927, 2128
Tth111I	GACNNNGTC	1 cut(s)	1633
VpaK11BI	GGWCC	3 cut(s)	1222, 1516, 2203
XagI	CCTNNNNNAGG	1 cut(s)	1279
XapI	RAATTY	5 cut(s)	272, 279, 1177, 2115, 2242
XbaI	TCTAGA	1 cut(s)	985
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1952
XhoI	CTCGAG	1 cut(s)	1981
XmiI	GTMKAC	1 cut(s)	927
XmnI	GAANNNNTTC	1 cut(s)	2151
XspI	CTAG	6 cut(s)	870, 939, 986, 1092, 1113, 1623
Zsp2I	ATGCAT	3 cut(s)	466, 826, 1864

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.