Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2C

Orthogroup ID

OG0009530

Physical Location & Seq

Genomic Coordinates Forward (+)

56617812 .. 56622154

Gene Structure 4343 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2CG414300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2304 bp

ATGGAGGGAAAAGTACATAGGTTTCTCCATGGAACACTTGAAGCTACCATCTTCCATGCCACACCTTACATACCACCACCATTCCCCTTCAATTGTATATTTGGAAATGGGAAGCCAGCGTATGTGACCATCAAGATAGACAACAAGAAAGTAGCAAAAACAACCCAAGAACGCGACCGCGTTTGGAACCAAACCTTTCAAATCCTATGTGCTCATTCATCATATTCAACCATTACCATTACAATGAAAACAAAATGCACCATCTTGGGGAAATTTCAAATCCATGCTCATCAGATACTAAGTAAAGCAAGCTTTATTAATGGTAATCTCCCTCTCCTAACAGAAAGTGGGAAGCCAAATCCAGAACTTAAGCTCCGGTTCATGTTATGGTTTAAGCCGGCGGAACTTGAACCAAGTTGGGGGAAAATGATTAGCTATGGTGAATTCCAAGGGCTGAGGAATGCAACATTTCCACAGAGATCCAATAGTCATGTCATTCTTTATCAAGATGCTCATCATTGCTCAAGTTTCAAGCCTTCGCCTGAGTTTTGTGGGACTCCAAGAAGACTATGGGAGGATGTATACAAAGCTATTGAGGGTGCAGAACATTTGATTTACATTGCAGGCTGGTCTTTTAATCCAAAAATGGTCCTGGTCAGGGATCCTCAAACAGCAATCCCACATGCAAGAGGAGTGAAGCTTGGTGAACTTTTGAAACAGAAAGCTGAGGAAGGCGTGGCGGTGAGAATTATGCTTTGGGATGATGAAACATCTTTACCACTTATCAAGAATGAAGGAATTATGAGAACACATGATGAAGATGCATTTGCCTACTTTTCACACACCCAAGTAGTGTGCAAATTGTGCCCAAGGTTGCACCGTAAGTTCCCCACAATCTTCTCTCACCATCAGAAAACAATAACCGTAGACACTAAATGTTGCATTTGTCCAAGGGAGAGAGAAATTATGAGCTTCATTGGTGGGCTGGATCTATGTGATGGCCGCTATGACACCGAGCAACATTCATTGTTCCACACACTCAACACAGAATCACATTGCTATGATTTCTACCAGACGAACATTGCGGGGTCTAGCCTCCAAAAAGGAGGGCCAAGAACACCATGGCACGATGTTCATGCTTGTATAACCGGGGAGGCTGCTTGGGATGTGCTAACGAATTTCGAGCAACGATGGAGTAAACAATGTGATCCTTCTGTATTGGTTCCTGCTAGCACCTTAACAAGCTTGATTCGGCAGACTTATGCAAGTAAACCCTCTGAAAGGGGTTGGAATGTTCAAGTGCTCAGATCAATTGACCACGTTTCGGCATCCCAATTGTTCCGAAACTTGACTGTGGAGCAAAGCATTCATGAGGCTTATGTGAAAGCAATTAGAGGTGCCGATAGGTTTATCTACATTGAGAACCAATATTTCACAGGAGGGTGTCATTTGTGGGAGAGGGACCAACATTGTGGGTGTATAAACTTGATTCCAATTGAGATTGCACTCAAGGTGGCCAATAAGATAAAAGCAAAGGAGCGGTTTGGAGTGTACATAGTAATACCAATGTGGCCAGAAGGTCTACCTGAGAGTGAATCAGTTCAGGATATACTTCATTTGACTAGAGAAACAATGTCAATGATGTACAAGATGATTGGAGAAGCTATAAAAGAAAGTGGGACTCAGCAGCACCCAAGAGACTATTTGAACTTCTATTGCCTTGCCAATAGAGAAGTAGAGACACAAGGAGACTTTGTCCCTCCACATTCTCCTCACAGTGGAACACAATACTGGAATGCACAGAAGCATAGGAGGTTCATGGTGTATGTTCACTCCAAGCTCATGATAGTGGACGATGCATACCTTATCATAGGATCTGCAAACATAAACCAAAGATCAATGGATGGAAAACGAGACACCGAGATTGCCATCGGATGCTACCAGACAATCAGAGACGATGATCATAACAGCAAGACTATAAGGATGAGCTCTACAGGGGACATTGGAGCATACCGAATGTCATTATGGTACGAGCACACAGGCCGTGCTGAAGAGTTGTACGAACACCCCGAAAGCTTAGAATGCGTGAAGACAGTGCGTTACATCGGAGACCAGATGTGGAAAACTTACAGCGGTGAAGAAGTGGTCGACATGGAAGGTGTTTACCTTGTGACATACCCTATGAGTGTAACAGAGGACGGTTTTGTTGAGGAGCTTGTGGAAGGAGGTGGTCTTTTTCCTGATACAAAGAGTTTGGTTAAGGGCAAGAGATCAAAAGTTTTACTGCCAATATTTACTACCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004620 GO:0004630 GO:0005575 GO:0005623 GO:0005886 GO:0006629 GO:0006644 GO:0006793 GO:0006796 GO:0006808 GO:0006950 GO:0006970 GO:0006995 GO:0007154 GO:0007275 GO:0008081 GO:0008150 GO:0008152 GO:0009056 GO:0009267 GO:0009395 GO:0009605 GO:0009628 GO:0009791 GO:0009825 GO:0009987 GO:0009991 GO:0016020 GO:0016036 GO:0016042 GO:0016049 GO:0016298 GO:0016787 GO:0016788 GO:0019637 GO:0022622 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0033554 GO:0040007 GO:0042578 GO:0042594 GO:0043562 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044464 GO:0045848 GO:0046434 GO:0048364 GO:0048518 GO:0048731 GO:0048856 GO:0050789 GO:0050896 GO:0051301 GO:0051365 GO:0051716 GO:0065007 GO:0071496 GO:0071704 GO:0071944 GO:0099402 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

C2 PLD_C PLDc

Protein Analysis

767

Amino Acids

87.74

Weight (kDa)

6.93

Isoelectric Point (pI)

40.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PLDc_2	PF13091	606 - 644	1.8e-06	PLD-like domain
PLDc	PF00614	610 - 633	1.2e-06	Phospholipase D Active site motif
PLD_C	PF12357	684 - 756	2.2e-22	Phospholipase D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009530)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55180 AT1G55180
fragaria_vesca	FvH4_6g33140
malus_domestica	MD09G1194700.v1.1 MD17G1175200.v1.1
prunus_persica	Prupe.3G051200_v2.0.a1
pyrus_communis	pycom09g11160
rosa_chinensis	RchiOBHm_Chr2g0142831
rosa_laevigata	RLG00000019989
rosa_multiflora	Rmu_co8241083.1_g000001 Rmu_co8446503.1_g000001 Rmu_sc0012306.1_g000009 Rmu_sc0013116.1_g000004
rosa_roxburghii	Rroxscaffold_2G00102280 Rroxscaffold_2G00103320
rosa_rugosa	Rorug02G0375600 Rorug02G0375700
rosa_samantha	Rh2AG428800 Rh2BG438100 Rh2CG414300 Rh2DG447900
rosa_wichuraiana	Rw2G035060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1397
AccBSI	CCGCTC	1 cut(s)	1540
AccI	GTMKAC	4 cut(s)	582, 927, 1582, 2148
AccII	CGCG	2 cut(s)	174, 180
AciI	CCGC	7 cut(s)	178, 401, 740, 1003, 1085, 1540, 2133
AclWI	GGATC	6 cut(s)	474, 656, 669, 996, 1202, 1882
AcoI	YGGCCR	3 cut(s)	1000, 1515, 1571
AcsI	RAATTY	3 cut(s)	272, 443, 1177
AcuI	CTGAAG	1 cut(s)	2070
AfaI	GTAC	5 cut(s)	15, 1553, 1646, 2030, 2060
AfiI	CCNNNNNNNGG	6 cut(s)	267, 419, 658, 1281, 1324, 1778
AflII	CTTAAG	1 cut(s)	368
AjnI	CCWGG	1 cut(s)	651
AjuI	GAANNNNNNNTTGG	2 cut(s)	739, 771
AloI	GAACNNNNNNTCC	2 cut(s)	357, 389
Alw21I	GWGCWC	4 cut(s)	214, 1305, 1991, 2037
Alw26I	GTCTC	6 cut(s)	1692, 1733, 1743, 1908, 1947, 2103
AlwI	GGATC	6 cut(s)	474, 656, 669, 996, 1202, 1882
AoxI	GGCC	5 cut(s)	1000, 1109, 1515, 1571, 2041
ApeKI	GCWGC	2 cut(s)	1157, 1687
ApoI	RAATTY	3 cut(s)	272, 443, 1177
AseI	ATTAAT	1 cut(s)	318
Asp700I	GAANNNNTTC	1 cut(s)	1599
AspS9I	GGNCC	3 cut(s)	649, 1109, 1462
AsuC2I	CCSGG	1 cut(s)	1150
AsuHPI	GGTGA	5 cut(s)	452, 716, 754, 896, 2147
AsuNHI	GCTAGC	1 cut(s)	1229
AvaII	GGWCC	2 cut(s)	649, 1462
BaeGI	GKGCMC	1 cut(s)	869
BalI	TGGCCA	2 cut(s)	1517, 1573
BamHI	GGATCC	1 cut(s)	661
BanI	GGYRCC	1 cut(s)	1397
BanII	GRGCYC	1 cut(s)	1991
BbsI	GAAGAC	2 cut(s)	571, 2096
Bbv12I	GWGCWC	4 cut(s)	214, 1305, 1991, 2037
BbvCI	CCTCAGC	2 cut(s)	455, 726
BbvI	GCAGC	2 cut(s)	1144, 1699
BccI	CCATC	8 cut(s)	56, 137, 269, 915, 992, 1185, 1898, 1937
BceAI	ACGGC	1 cut(s)	2028
BcgI	CGANNNNNNTGC	2 cut(s)	1118, 1152
BciT130I	CCWGG	1 cut(s)	653
BclI	TGATCA	1 cut(s)	1960
BcnI	CCSGG	1 cut(s)	1150
BcoDI	GTCTC	6 cut(s)	1692, 1733, 1743, 1908, 1947, 2103
BfaI	CTAG	4 cut(s)	1092, 1230, 1623, 2302
BfmI	CTRYAG	1 cut(s)	1992
BfrI	CTTAAG	1 cut(s)	368
BisI	GCNGC	3 cut(s)	1003, 1158, 1688
BlsI	GCNGC	3 cut(s)	1004, 1159, 1689
Bme1390I	CCNGG	2 cut(s)	653, 1150
Bme18I	GGWCC	2 cut(s)	649, 1462
BmgT120I	GGNCC	3 cut(s)	649, 1109, 1462
BmiI	GGNNCC	5 cut(s)	188, 663, 1224, 1399, 1463
BmrFI	CCNGG	2 cut(s)	653, 1150
BmsI	GCATC	5 cut(s)	499, 811, 1337, 1846, 1925
BmtI	GCTAGC	1 cut(s)	1233
BpiI	GAAGAC	2 cut(s)	571, 2096
Bpu10I	CCTNAGC	2 cut(s)	455, 726
BpuEI	CTTGAG	2 cut(s)	508, 1493
BpuMI	CCSGG	1 cut(s)	1150
BsaBI	GATNNNNATC	2 cut(s)	513, 1928
BsaI	GGTCTC	1 cut(s)	2103
BsaJI	CCNNGG	6 cut(s)	28, 448, 869, 950, 1121, 1149
BsaWI	WCCGGW	1 cut(s)	375
BsaXI	ACNNNNNCTCC	4 cut(s)	357, 387, 1740, 1770
Bsc4I	CCNNNNNNNGG	6 cut(s)	267, 419, 658, 1281, 1324, 1778
Bse118I	RCCGGY	1 cut(s)	397
Bse1I	ACTGG	1 cut(s)	1796
Bse3DI	GCAATG	4 cut(s)	517, 618, 1054, 1080
Bse8I	GATNNNNATC	2 cut(s)	513, 1928
BseBI	CCWGG	1 cut(s)	653
BseDI	CCNNGG	6 cut(s)	28, 448, 869, 950, 1121, 1149
BseGI	GGATG	7 cut(s)	583, 766, 1171, 1328, 1909, 1940, 1989
BseJI	GATNNNNATC	2 cut(s)	513, 1928
BseLI	CCNNNNNNNGG	6 cut(s)	267, 419, 658, 1281, 1324, 1778
BseMI	GCAATG	4 cut(s)	517, 618, 1054, 1080
BseMII	CTCAG	6 cut(s)	446, 534, 717, 1318, 1578, 1697
BseNI	ACTGG	1 cut(s)	1796
BseRI	GAGGAG	3 cut(s)	705, 1761, 2225
BseSI	GKGCMC	1 cut(s)	869
BseXI	GCAGC	2 cut(s)	1144, 1699
BsgI	GTGCAG	1 cut(s)	621
Bsh1236I	CGCG	2 cut(s)	174, 180
Bsh1285I	CGRYCG	1 cut(s)	178
BshFI	GGCC	5 cut(s)	1002, 1111, 1517, 1573, 2043
BshNI	GGYRCC	1 cut(s)	1397
BsiEI	CGRYCG	1 cut(s)	178
BsiHKAI	GWGCWC	4 cut(s)	214, 1305, 1991, 2037
BsiSI	CCGG	3 cut(s)	376, 398, 1149
BslFI	GGGAC	5 cut(s)	568, 1475, 1693, 1742, 2012
BslI	CCNNNNNNNGG	6 cut(s)	267, 419, 658, 1281, 1324, 1778
BsmAI	GTCTC	6 cut(s)	1692, 1733, 1743, 1908, 1947, 2103
BsmBI	CGTCTC	1 cut(s)	1947
BsmFI	GGGAC	5 cut(s)	568, 1475, 1693, 1742, 2012
BsmI	GAATGC	4 cut(s)	466, 1365, 1801, 2087
BsnI	GGCC	5 cut(s)	1002, 1111, 1517, 1573, 2043
Bso31I	GGTCTC	1 cut(s)	2103
Bsp1286I	GDGCHC	5 cut(s)	214, 869, 1305, 1991, 2037
Bsp1407I	TGTACA	2 cut(s)	1551, 1644
Bsp143I	GATC	9 cut(s)	479, 661, 988, 1207, 1307, 1874, 1895, 1960, 2270
Bsp19I	CCATGG	2 cut(s)	28, 1121
BspACI	CCGC	7 cut(s)	178, 401, 740, 1003, 1085, 1540, 2133
BspANI	GGCC	5 cut(s)	1002, 1111, 1517, 1573, 2043
BspCNI	CTCAG	6 cut(s)	447, 535, 718, 1317, 1579, 1696
BspFNI	CGCG	2 cut(s)	174, 180
BspHI	TCATGA	2 cut(s)	1369, 1842
BspLI	GGNNCC	5 cut(s)	188, 663, 1224, 1399, 1463
BspOI	GCTAGC	1 cut(s)	1233
BspPI	GGATC	6 cut(s)	474, 656, 669, 996, 1202, 1882
BspT107I	GGYRCC	1 cut(s)	1397
BspTI	CTTAAG	1 cut(s)	368
BspTNI	GGTCTC	1 cut(s)	2103
BsrBI	CCGCTC	1 cut(s)	1540
BsrDI	GCAATG	4 cut(s)	517, 618, 1054, 1080
BsrFI	RCCGGY	1 cut(s)	397
BsrGI	TGTACA	2 cut(s)	1551, 1644
BsrI	ACTGG	1 cut(s)	1796
BssAI	RCCGGY	1 cut(s)	397
BssECI	CCNNGG	6 cut(s)	28, 448, 869, 950, 1121, 1149
BssMI	GATC	9 cut(s)	479, 661, 988, 1207, 1307, 1874, 1895, 1960, 2270
BssNAI	GTATAC	1 cut(s)	583
BssT1I	CCWWGG	5 cut(s)	28, 448, 869, 950, 1121
Bst1107I	GTATAC	1 cut(s)	583
Bst2UI	CCWGG	1 cut(s)	653
Bst4CI	ACNGT	6 cut(s)	881, 925, 1354, 1778, 2095, 2201
Bst6I	CTCTTC	1 cut(s)	2046
BstAFI	CTTAAG	1 cut(s)	368
BstAPI	GCANNNNNTGC	1 cut(s)	864
BstAUI	TGTACA	2 cut(s)	1551, 1644
BstC8I	GCNNGC	5 cut(s)	117, 310, 399, 625, 1231
BstDEI	CTNAG	8 cut(s)	299, 455, 543, 726, 1304, 1587, 1683, 2077
BstDSI	CCRYGG	2 cut(s)	28, 1121
BstENI	CCTNNNNNAGG	1 cut(s)	1279
BstF5I	GGATG	7 cut(s)	583, 766, 1171, 1328, 1909, 1940, 1989
BstFNI	CGCG	2 cut(s)	174, 180
BstKTI	GATC	9 cut(s)	482, 664, 991, 1210, 1310, 1877, 1898, 1963, 2273
BstMAI	GTCTC	6 cut(s)	1692, 1733, 1743, 1908, 1947, 2103
BstMBI	GATC	9 cut(s)	479, 661, 988, 1207, 1307, 1874, 1895, 1960, 2270
BstMCI	CGRYCG	1 cut(s)	178
BstMWI	GCNNNNNNNGC	2 cut(s)	864, 2082
BstNI	CCWGG	1 cut(s)	653
BstNSI	RCATGY	1 cut(s)	686
BstSCI	CCNGG	2 cut(s)	651, 1148
BstSFI	CTRYAG	1 cut(s)	1992
BstSLI	GKGCMC	1 cut(s)	869
BstUI	CGCG	2 cut(s)	174, 180
BstV1I	GCAGC	2 cut(s)	1144, 1699
BstV2I	GAAGAC	2 cut(s)	571, 2096
BstX2I	RGATCY	4 cut(s)	479, 661, 988, 1874
BstXI	CCANNNNNNTGG	1 cut(s)	1472
BstYI	RGATCY	4 cut(s)	479, 661, 988, 1874
BstZ17I	GTATAC	1 cut(s)	583
BsuRI	GGCC	5 cut(s)	1002, 1111, 1517, 1573, 2043
BtgI	CCRYGG	2 cut(s)	28, 1121
BtsCI	GGATG	7 cut(s)	583, 766, 1171, 1328, 1909, 1940, 1989
BtsIMutI	CAGTG	2 cut(s)	1783, 2100
Cac8I	GCNNGC	5 cut(s)	117, 310, 399, 625, 1231
CciI	TCATGA	2 cut(s)	1369, 1842
Cfr10I	RCCGGY	1 cut(s)	397
Cfr13I	GGNCC	3 cut(s)	649, 1109, 1462
Csp6I	GTAC	5 cut(s)	14, 1552, 1645, 2029, 2059
CviQI	GTAC	5 cut(s)	14, 1552, 1645, 2029, 2059
DdeI	CTNAG	8 cut(s)	299, 455, 543, 726, 1304, 1587, 1683, 2077
DpnI	GATC	9 cut(s)	481, 663, 990, 1209, 1309, 1876, 1897, 1962, 2272
DpnII	GATC	9 cut(s)	479, 661, 988, 1207, 1307, 1874, 1895, 1960, 2270
EaeI	YGGCCR	3 cut(s)	1000, 1515, 1571
Eam1104I	CTCTTC	1 cut(s)	2046
EarI	CTCTTC	1 cut(s)	2046
EciI	GGCGGA	1 cut(s)	416
Ecl136II	GAGCTC	1 cut(s)	1989
Eco130I	CCWWGG	5 cut(s)	28, 448, 869, 950, 1121
Eco24I	GRGCYC	1 cut(s)	1991
Eco31I	GGTCTC	1 cut(s)	2103
Eco47I	GGWCC	2 cut(s)	649, 1462
Eco53kI	GAGCTC	1 cut(s)	1989
Eco57I	CTGAAG	1 cut(s)	2070
EcoICRI	GAGCTC	1 cut(s)	1989
EcoNI	CCTNNNNNAGG	1 cut(s)	1279
EcoRI	GAATTC	1 cut(s)	443
EcoRII	CCWGG	1 cut(s)	651
EcoT14I	CCWWGG	5 cut(s)	28, 448, 869, 950, 1121
EcoT22I	ATGCAT	2 cut(s)	826, 1861
EcoT38I	GRGCYC	1 cut(s)	1991
ErhI	CCWWGG	5 cut(s)	28, 448, 869, 950, 1121
Esp3I	CGTCTC	1 cut(s)	1947
FaqI	GGGAC	5 cut(s)	568, 1475, 1693, 1742, 2012
FauI	CCCGC	1 cut(s)	1078
FbaI	TGATCA	1 cut(s)	1960
FblI	GTMKAC	4 cut(s)	582, 927, 1582, 2148
Fnu4HI	GCNGC	3 cut(s)	1003, 1158, 1688
FokI	GGATG	7 cut(s)	590, 773, 1178, 1315, 1916, 1947, 1996
FriOI	GRGCYC	1 cut(s)	1991
Fsp4HI	GCNGC	3 cut(s)	1003, 1158, 1688
FspBI	CTAG	4 cut(s)	1092, 1230, 1623, 2302
GluI	GCNGC	3 cut(s)	1003, 1158, 1688
HaeIII	GGCC	5 cut(s)	1002, 1111, 1517, 1573, 2043
HapII	CCGG	3 cut(s)	376, 398, 1149
HincII	GTYRAC	1 cut(s)	2149
HindII	GTYRAC	1 cut(s)	2149
HindIII	AAGCTT	4 cut(s)	310, 698, 1243, 2074
HinfI	GANTC	6 cut(s)	556, 1049, 1249, 1489, 1595, 1681
HpaII	CCGG	3 cut(s)	376, 398, 1149
HphI	GGTGA	5 cut(s)	452, 716, 754, 896, 2147
Hpy188I	TCNGA	8 cut(s)	294, 912, 1279, 1307, 1343, 1934, 1952, 2108
Hpy188III	TCNNGA	8 cut(s)	133, 362, 506, 787, 1370, 1604, 1843, 2240
HpyAV	CCTTC	8 cut(s)	97, 546, 725, 788, 1221, 1571, 2150, 2216
HpyCH4III	ACNGT	6 cut(s)	881, 925, 1354, 1778, 2095, 2201
HpyCH4IV	ACGT	1 cut(s)	1320
HpyF10VI	GCNNNNNNNGC	2 cut(s)	864, 2082
HpyF3I	CTNAG	8 cut(s)	299, 455, 543, 726, 1304, 1587, 1683, 2077
HpySE526I	ACGT	1 cut(s)	1320
KroI	GCCGGC	1 cut(s)	397
KroNI	GCCGGC	1 cut(s)	399
Ksp22I	TGATCA	1 cut(s)	1960
Kzo9I	GATC	9 cut(s)	479, 661, 988, 1207, 1307, 1874, 1895, 1960, 2270
LmnI	GCTCC	5 cut(s)	378, 1357, 1537, 2006, 2212
Lsp1109I	GCAGC	2 cut(s)	1144, 1699
LweI	GCATC	5 cut(s)	499, 811, 1337, 1846, 1925
MaeI	CTAG	4 cut(s)	1092, 1230, 1623, 2302
MaeII	ACGT	1 cut(s)	1320
MaeIII	GTNAC	4 cut(s)	124, 2099, 2170, 2188
MalI	GATC	9 cut(s)	481, 663, 990, 1209, 1309, 1876, 1897, 1962, 2272
MbiI	CCGCTC	1 cut(s)	1540
MboI	GATC	9 cut(s)	479, 661, 988, 1207, 1307, 1874, 1895, 1960, 2270
MboII	GAAGA	7 cut(s)	43, 576, 830, 889, 2063, 2101, 2150
MfeI	CAATTG	4 cut(s)	91, 1311, 1334, 1494
MflI	RGATCY	4 cut(s)	479, 661, 988, 1874
MhlI	GDGCHC	5 cut(s)	214, 869, 1305, 1991, 2037
MlsI	TGGCCA	2 cut(s)	1517, 1573
MluNI	TGGCCA	2 cut(s)	1517, 1573
MlyI	GAGTC	2 cut(s)	550, 1675
MmeI	TCCRAC	1 cut(s)	1268
Mox20I	TGGCCA	2 cut(s)	1517, 1573
Mph1103I	ATGCAT	2 cut(s)	826, 1861
MroNI	GCCGGC	1 cut(s)	397
MroXI	GAANNNNTTC	1 cut(s)	1599
MscI	TGGCCA	2 cut(s)	1517, 1573
MseI	TTAA	6 cut(s)	318, 369, 393, 636, 1238, 2259
MslI	CAYNNNNRTG	3 cut(s)	242, 1059, 1847
Msp20I	TGGCCA	2 cut(s)	1517, 1573
MspA1I	CMGCKG	1 cut(s)	2133
MspCI	CTTAAG	1 cut(s)	368
MspI	CCGG	3 cut(s)	376, 398, 1149
MspR9I	CCNGG	2 cut(s)	653, 1150
MunI	CAATTG	4 cut(s)	91, 1311, 1334, 1494
Mva1269I	GAATGC	4 cut(s)	466, 1365, 1801, 2087
MvaI	CCWGG	1 cut(s)	653
MvnI	CGCG	2 cut(s)	174, 180
MwoI	GCNNNNNNNGC	2 cut(s)	864, 2082
NaeI	GCCGGC	1 cut(s)	399
NciI	CCSGG	1 cut(s)	1150
NcoI	CCATGG	2 cut(s)	28, 1121
NdeII	GATC	9 cut(s)	479, 661, 988, 1207, 1307, 1874, 1895, 1960, 2270
NgoMIV	GCCGGC	1 cut(s)	397
NheI	GCTAGC	1 cut(s)	1229
NlaIV	GGNNCC	5 cut(s)	188, 663, 1224, 1399, 1463
NmuCI	GTSAC	2 cut(s)	124, 2170
NsiI	ATGCAT	2 cut(s)	826, 1861
NspI	RCATGY	1 cut(s)	686
PagI	TCATGA	2 cut(s)	1369, 1842
PcsI	WCGNNNNNNNCGW	1 cut(s)	2067
PctI	GAATGC	4 cut(s)	466, 1365, 1801, 2087
PdiI	GCCGGC	1 cut(s)	399
PdmI	GAANNNNTTC	1 cut(s)	1599
PfeI	GAWTC	4 cut(s)	1049, 1249, 1489, 1595
PflFI	GACNNNGTC	1 cut(s)	1754
PkrI	GCNGC	3 cut(s)	1004, 1159, 1689
PleI	GAGTC	2 cut(s)	550, 1675
PpsI	GAGTC	2 cut(s)	550, 1675
PshBI	ATTAAT	1 cut(s)	318
Psp124BI	GAGCTC	1 cut(s)	1991
Psp6I	CCWGG	1 cut(s)	651
PspGI	CCWGG	1 cut(s)	651
PspN4I	GGNNCC	5 cut(s)	188, 663, 1224, 1399, 1463
PspPI	GGNCC	3 cut(s)	649, 1109, 1462
PsuI	RGATCY	4 cut(s)	479, 661, 988, 1874
PsyI	GACNNNGTC	1 cut(s)	1754
RsaI	GTAC	5 cut(s)	15, 1553, 1646, 2030, 2060
RsaNI	GTAC	5 cut(s)	14, 1552, 1645, 2029, 2059
RseI	CAYNNNNRTG	3 cut(s)	242, 1059, 1847
SacI	GAGCTC	1 cut(s)	1991
SalI	GTCGAC	1 cut(s)	2147
SaqAI	TTAA	6 cut(s)	318, 369, 393, 636, 1238, 2259
SatI	GCNGC	3 cut(s)	1003, 1158, 1688
Sau3AI	GATC	9 cut(s)	479, 661, 988, 1207, 1307, 1874, 1895, 1960, 2270
Sau96I	GGNCC	3 cut(s)	649, 1109, 1462
SchI	GAGTC	2 cut(s)	550, 1675
ScrFI	CCNGG	2 cut(s)	653, 1150
SduI	GDGCHC	5 cut(s)	214, 869, 1305, 1991, 2037
SfaNI	GCATC	5 cut(s)	499, 811, 1337, 1846, 1925
SfcI	CTRYAG	1 cut(s)	1992
SinI	GGWCC	2 cut(s)	649, 1462
SmiMI	CAYNNNNRTG	3 cut(s)	242, 1059, 1847
SmlI	CTYRAG	3 cut(s)	368, 523, 1508
SmoI	CTYRAG	3 cut(s)	368, 523, 1508
SsiI	CCGC	7 cut(s)	178, 401, 740, 1003, 1085, 1540, 2133
SspI	AATATT	2 cut(s)	1430, 2292
SspMI	CTAG	4 cut(s)	1092, 1230, 1623, 2302
SstI	GAGCTC	1 cut(s)	1991
StyD4I	CCNGG	2 cut(s)	651, 1148
StyI	CCWWGG	5 cut(s)	28, 448, 869, 950, 1121
TaaI	ACNGT	6 cut(s)	881, 925, 1354, 1778, 2095, 2201
TaiI	ACGT	1 cut(s)	1323
TaqI	TCGA	2 cut(s)	1182, 2148
TatI	WGTACW	3 cut(s)	13, 1551, 1644
TauI	GCSGC	1 cut(s)	1005
TfiI	GAWTC	4 cut(s)	1049, 1249, 1489, 1595
Tru1I	TTAA	6 cut(s)	318, 369, 393, 636, 1238, 2259
Tru9I	TTAA	6 cut(s)	318, 369, 393, 636, 1238, 2259
TscAI	CASTG	2 cut(s)	1783, 2100
TseFI	GTSAC	2 cut(s)	124, 2170
TseI	GCWGC	2 cut(s)	1157, 1687
Tsp45I	GTSAC	2 cut(s)	124, 2170
TspRI	CASTG	2 cut(s)	1783, 2100
Tth111I	GACNNNGTC	1 cut(s)	1754
Vha464I	CTTAAG	1 cut(s)	368
VpaK11BI	GGWCC	2 cut(s)	649, 1462
VspI	ATTAAT	1 cut(s)	318
XagI	CCTNNNNNAGG	1 cut(s)	1279
XapI	RAATTY	3 cut(s)	272, 443, 1177
XceI	RCATGY	1 cut(s)	686
XcmI	CCANNNNNNNNNTGG	2 cut(s)	567, 1119
XmiI	GTMKAC	4 cut(s)	582, 927, 1582, 2148
XmnI	GAANNNNTTC	1 cut(s)	1599
XspI	CTAG	4 cut(s)	1092, 1230, 1623, 2302
Zsp2I	ATGCAT	2 cut(s)	826, 1861

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.