MD09G1225800.v1.1

Chaperone protein dnaJ 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0013509
Physical Location & Seq
Reverse (-)
27854652 .. 27856807
2156 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1225800.v1.1.491

Sequence Viewer

Length: 471 bp
ATGGCGTGTGTTGGAGCAGGTGTGATTGGGGGCAATGGGTCGTCCTGGAATCTGTTCAAGGACAAGGCAAGGAAGAAGAAGATGGTGAATAATAATAAGAGGGTGATGGTTTCTTGTGTTGCGTCGTCCGTGATCGATCCTTACCGGACTTTGAAGATTCAACCCGGTGCCTCTGAATCTGAGGTCAGGAAGGCGTTTAGACAACTTGCCTTGAAGTATCATCCAGACGTATGCAGAGGAAACAATTGTGGAGTTGAGTTTCACCAGATCAATGAAGCCTATGATATTGTTATGAGCAATTTGAGAGGAGAAACGATGACTCCATTGCAGATGTATGAGACGTGCGATGAAGGGGATGAAGAGCCGATGAGGGGAATGAATGATCCAGATTGGGACATGTGGGAGGAATGGATGGGATGGGAAGGAGCTGGAATTAGAGACTACACATCTCACATTAATCCTTATATTTGA

Protein Analysis

157

Amino Acids

17.72

Weight (kDa)

5.4

Isoelectric Point (pI)

36.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 46 - 98 6.3e-15 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013509)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80920
fragaria_vesca FvH4_6g28480
malus_domestica MD09G1225800.v1.1 MD17G1224600.v1.1
prunus_persica Prupe.3G100300_v2.0.a1
pyrus_communis pycom09g14530 pycom17g22830
rosa_chinensis RchiOBHm_Chr2g0132701
rosa_laevigata RLG00000019273
rosa_multiflora Rmu_ssc0000074.1_g000011
rosa_roxburghii Rroxscaffold_2G00111720
rosa_rugosa Rorug02G0306300
rosa_samantha Rh2AG359400 Rh2BG365700 Rh2CG342500 Rh2DG382400
rosa_wichuraiana Rw2G029240 Rw3G024410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 8
Acc36I ACCTGC 1 cut(s) 8
AccB1I GGYRCC 1 cut(s) 167
AclWI GGATC 2 cut(s) 131, 377
AfiI CCNNNNNNNGG 1 cut(s) 371
AflIII ACRYGT 1 cut(s) 396
AgsI TTSAA 4 cut(s) 58, 154, 161, 214
AjiI CACGTC 1 cut(s) 342
AjnI CCWGG 1 cut(s) 44
AluBI AGCT 1 cut(s) 428
AluI AGCT 1 cut(s) 428
Alw26I GTCTC 2 cut(s) 332, 432
AlwI GGATC 2 cut(s) 131, 377
AseI ATTAAT 1 cut(s) 456
Asp700I GAANNNNTTC 1 cut(s) 53
AsuC2I CCSGG 1 cut(s) 165
AsuHPI GGTGA 3 cut(s) 97, 115, 254
BanI GGYRCC 1 cut(s) 167
BccI CCATC 4 cut(s) 76, 100, 406, 411
BciT130I CCWGG 1 cut(s) 46
BcnI CCSGG 1 cut(s) 165
BcoDI GTCTC 2 cut(s) 332, 432
BfuAI ACCTGC 1 cut(s) 8
Bme1390I CCNGG 2 cut(s) 46, 165
BmgBI CACGTC 1 cut(s) 342
BmiI GGNNCC 1 cut(s) 169
BmrFI CCNGG 2 cut(s) 46, 165
BpuMI CCSGG 1 cut(s) 165
Bsa29I ATCGAT 1 cut(s) 135
BsaWI WCCGGW 1 cut(s) 144
BsaXI ACNNNNNCTCC 4 cut(s) 6, 36, 304, 334
Bsc4I CCNNNNNNNGG 1 cut(s) 371
Bse3DI GCAATG 2 cut(s) 40, 323
BseBI CCWGG 1 cut(s) 46
BseCI ATCGAT 1 cut(s) 135
BseGI GGATG 4 cut(s) 220, 361, 417, 422
BseLI CCNNNNNNNGG 1 cut(s) 371
BseMI GCAATG 2 cut(s) 40, 323
BseMII CTCAG 1 cut(s) 171
BseRI GAGGAG 1 cut(s) 321
BshNI GGYRCC 1 cut(s) 167
BshVI ATCGAT 1 cut(s) 135
BsiSI CCGG 2 cut(s) 145, 165
BslFI GGGAC 1 cut(s) 407
BslI CCNNNNNNNGG 1 cut(s) 371
BsmAI GTCTC 2 cut(s) 332, 432
BsmBI CGTCTC 1 cut(s) 332
BsmFI GGGAC 1 cut(s) 407
Bsp143I GATC 4 cut(s) 132, 136, 267, 382
BspCNI CTCAG 1 cut(s) 172
BspDI ATCGAT 1 cut(s) 135
BspLI GGNNCC 1 cut(s) 169
BspMI ACCTGC 1 cut(s) 8
BspPI GGATC 2 cut(s) 131, 377
BspQI GCTCTTC 1 cut(s) 354
BspT107I GGYRCC 1 cut(s) 167
BsrDI GCAATG 2 cut(s) 40, 323
BssMI GATC 4 cut(s) 132, 136, 267, 382
Bst2UI CCWGG 1 cut(s) 46
Bst6I CTCTTC 1 cut(s) 354
BstDEI CTNAG 1 cut(s) 180
BstF5I GGATG 4 cut(s) 220, 361, 417, 422
BstKTI GATC 4 cut(s) 135, 139, 270, 385
BstMAI GTCTC 2 cut(s) 332, 432
BstMBI GATC 4 cut(s) 132, 136, 267, 382
BstNI CCWGG 1 cut(s) 46
BstNSI RCATGY 1 cut(s) 400
BstSCI CCNGG 2 cut(s) 44, 163
Bsu15I ATCGAT 1 cut(s) 135
BsuTUI ATCGAT 1 cut(s) 135
BtgZI GCGATG 1 cut(s) 360
BtrI CACGTC 1 cut(s) 342
BtsCI GGATG 4 cut(s) 220, 361, 417, 422
BveI ACCTGC 1 cut(s) 8
ClaI ATCGAT 1 cut(s) 135
CseI GACGC 1 cut(s) 111
CviAII CATG 1 cut(s) 397
CviJI RGCY 3 cut(s) 278, 364, 428
CviKI_1 RGCY 3 cut(s) 278, 364, 428
DdeI CTNAG 1 cut(s) 180
DpnI GATC 4 cut(s) 134, 138, 269, 384
DpnII GATC 4 cut(s) 132, 136, 267, 382
Eam1104I CTCTTC 1 cut(s) 354
EarI CTCTTC 1 cut(s) 354
EcoRII CCWGG 1 cut(s) 44
Esp3I CGTCTC 1 cut(s) 332
FaeI CATG 1 cut(s) 400
FaiI YATR 6 cut(s) 232, 282, 293, 336, 398, 465
FaqI GGGAC 1 cut(s) 407
FatI CATG 1 cut(s) 396
FokI GGATG 4 cut(s) 207, 368, 424, 429
HapII CCGG 2 cut(s) 145, 165
HgaI GACGC 1 cut(s) 111
Hin1II CATG 1 cut(s) 400
HinfI GANTC 4 cut(s) 49, 157, 176, 319
HpaII CCGG 2 cut(s) 145, 165
HphI GGTGA 3 cut(s) 97, 115, 254
Hpy188I TCNGA 2 cut(s) 175, 181
Hpy188III TCNNGA 3 cut(s) 187, 224, 386
Hpy99I CGWCG 1 cut(s) 127
HpyAV CCTTC 3 cut(s) 184, 344, 416
HpyCH4IV ACGT 2 cut(s) 228, 341
HpyCH4V TGCA 2 cut(s) 234, 328
HpyF3I CTNAG 1 cut(s) 180
HpySE526I ACGT 2 cut(s) 228, 341
Hsp92II CATG 1 cut(s) 400
Kzo9I GATC 4 cut(s) 132, 136, 267, 382
LguI GCTCTTC 1 cut(s) 354
LmnI GCTCC 2 cut(s) 14, 425
MaeII ACGT 2 cut(s) 228, 341
MalI GATC 4 cut(s) 134, 138, 269, 384
MboI GATC 4 cut(s) 132, 136, 267, 382
MboII GAAGA 5 cut(s) 85, 88, 91, 166, 371
MfeI CAATTG 1 cut(s) 244
MluCI AATT 3 cut(s) 244, 298, 432
MlyI GAGTC 1 cut(s) 313
MnlI CCTC 7 cut(s) 93, 175, 181, 230, 299, 363, 397
MroXI GAANNNNTTC 1 cut(s) 53
MseI TTAA 1 cut(s) 456
MspI CCGG 2 cut(s) 145, 165
MspR9I CCNGG 2 cut(s) 46, 165
MunI CAATTG 1 cut(s) 244
MvaI CCWGG 1 cut(s) 46
NciI CCSGG 1 cut(s) 165
NdeII GATC 4 cut(s) 132, 136, 267, 382
NlaIII CATG 1 cut(s) 400
NlaIV GGNNCC 1 cut(s) 169
NspI RCATGY 1 cut(s) 400
PaqCI CACCTGC 1 cut(s) 8
PciI ACATGT 1 cut(s) 396
PciSI GCTCTTC 1 cut(s) 354
PdmI GAANNNNTTC 1 cut(s) 53
PfeI GAWTC 3 cut(s) 49, 157, 176
PfoI TCCNGGA 1 cut(s) 44
PleI GAGTC 1 cut(s) 313
PpsI GAGTC 1 cut(s) 313
PscI ACATGT 1 cut(s) 396
PshBI ATTAAT 1 cut(s) 456
Psp6I CCWGG 1 cut(s) 44
PspGI CCWGG 1 cut(s) 44
PspN4I GGNNCC 1 cut(s) 169
SapI GCTCTTC 1 cut(s) 354
SaqAI TTAA 1 cut(s) 456
Sau3AI GATC 4 cut(s) 132, 136, 267, 382
SchI GAGTC 1 cut(s) 313
ScrFI CCNGG 2 cut(s) 46, 165
SetI ASST 5 cut(s) 22, 186, 231, 344, 430
Sse9I AATT 3 cut(s) 244, 298, 432
StyD4I CCNGG 2 cut(s) 44, 163
TaiI ACGT 2 cut(s) 231, 344
TaqI TCGA 1 cut(s) 135
TasI AATT 3 cut(s) 244, 298, 432
TfiI GAWTC 3 cut(s) 49, 157, 176
Tru1I TTAA 1 cut(s) 456
Tru9I TTAA 1 cut(s) 456
TspDTI ATGAA 4 cut(s) 288, 363, 372, 392
TspGWI ACGGA 1 cut(s) 118
VspI ATTAAT 1 cut(s) 456
XceI RCATGY 1 cut(s) 400
XmnI GAANNNNTTC 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.