Rorug02G0306300

Chaperone protein dnaJ 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0013509
Physical Location & Seq
Forward (+)
34676161 .. 34679269
3109 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0306300.1

Sequence Viewer

Length: 663 bp
ATGGATCTCAAATCCGTCGGAGCCAAGTCGTTACCCAACCCAGCCATTCTTCGCAAGTCATCTCATCTTCTCAGTCCCAAAGTCCTTCAGACATTATGGGTAGCAATTAATGTTGACGATTTTGCGAGAAATTCTAGTCGGCGGCTTTTGAAACTTGGTCAAAGTGATGGACATGGTGAGGTTCCAGCTATAGAGTACTCGCATATCCCTTCGTTCTCGGACGACGTCATTTCGGGCACTAAGAGTTCATCAGTTGCTTCTTCTACGACGTCGTTTGGGAGCTCGAAGAAGATGGTGTGGGTTTGGACGGAGAGCAAGCAGGTCATGACGACCGCCGTGGAGAGAGGCTGGAACACCTTCCTGTTTCAGAGTCAGAAACTCGCCGATGACTGGTCTTGTGAGCTTATGAAATTTGAGTACCAAAAGAAGGTCGCCTCGCTGAGCAAGCTAAAGAAATGTAGTACTAACTCAGAAGCATTGGAGAAATCAAGAGCAGCAGTGAGTCATCTGCATATAAGATACATAGTTGACATGCAATCCATGGACCCAACAGTATCAAAGATAAACTCTTTTACTTTCAGTCTGTTGCAGGGTTTGGGAATATCTAGTGAAATGTTGCCTAAAGAGTCACTAAGTGATCAAGCATGGAATACCAAGCAATGA

Protein Analysis

220

Amino Acids

24.45

Weight (kDa)

9.28

Isoelectric Point (pI)

46.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF632 PF04782 134 - 190 8.4e-11 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013509)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80920
fragaria_vesca FvH4_6g28480
malus_domestica MD09G1225800.v1.1 MD17G1224600.v1.1
prunus_persica Prupe.3G100300_v2.0.a1
pyrus_communis pycom09g14530 pycom17g22830
rosa_chinensis RchiOBHm_Chr2g0132701
rosa_laevigata RLG00000019273
rosa_multiflora Rmu_ssc0000074.1_g000011
rosa_roxburghii Rroxscaffold_2G00111720
rosa_rugosa Rorug02G0306300
rosa_samantha Rh2AG359400 Rh2BG365700 Rh2CG342500 Rh2DG382400
rosa_wichuraiana Rw2G029240 Rw3G024410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 228, 272
Acc36I ACCTGC 1 cut(s) 310
AciI CCGC 2 cut(s) 142, 333
AclWI GGATC 1 cut(s) 12
AcsI RAATTY 2 cut(s) 130, 410
AcuI CTGAAG 1 cut(s) 71
AcyI GRCGYC 2 cut(s) 225, 269
AdeI CACNNNGTG 1 cut(s) 635
AfaI GTAC 3 cut(s) 197, 419, 463
AfiI CCNNNNNNNGG 2 cut(s) 390, 427
AgsI TTSAA 1 cut(s) 151
AluBI AGCT 4 cut(s) 188, 282, 403, 448
AluI AGCT 4 cut(s) 188, 282, 403, 448
Alw21I GWGCWC 1 cut(s) 284
AlwI GGATC 1 cut(s) 12
ApeKI GCWGC 1 cut(s) 494
ApoI RAATTY 2 cut(s) 130, 410
AseI ATTAAT 1 cut(s) 108
Asp700I GAANNNNTTC 1 cut(s) 356
AspS9I GGNCC 1 cut(s) 544
AsuHPI GGTGA 1 cut(s) 188
AvaII GGWCC 1 cut(s) 544
BaeGI GKGCMC 1 cut(s) 239
BanII GRGCYC 1 cut(s) 284
Bbv12I GWGCWC 1 cut(s) 284
BbvI GCAGC 1 cut(s) 506
BccI CCATC 2 cut(s) 161, 286
BceAI ACGGC 1 cut(s) 320
BclI TGATCA 1 cut(s) 637
BfaI CTAG 2 cut(s) 135, 606
BfmI CTRYAG 1 cut(s) 189
BfuAI ACCTGC 1 cut(s) 310
BisI GCNGC 2 cut(s) 143, 495
BlpI GCTNAGC 1 cut(s) 440
BlsI GCNGC 2 cut(s) 144, 496
BmcAI AGTACT 2 cut(s) 197, 463
Bme18I GGWCC 1 cut(s) 544
BmgT120I GGNCC 1 cut(s) 544
BmiI GGNNCC 3 cut(s) 22, 183, 546
Bpu1102I GCTNAGC 1 cut(s) 440
BsaHI GRCGYC 2 cut(s) 225, 269
BsaJI CCNNGG 2 cut(s) 336, 540
Bsc4I CCNNNNNNNGG 2 cut(s) 390, 427
Bse1I ACTGG 1 cut(s) 395
BseDI CCNNGG 2 cut(s) 336, 540
BseLI CCNNNNNNNGG 2 cut(s) 390, 427
BseMII CTCAG 3 cut(s) 85, 431, 483
BseNI ACTGG 1 cut(s) 395
BseSI GKGCMC 1 cut(s) 239
BseXI GCAGC 1 cut(s) 506
BseYI CCCAGC 1 cut(s) 40
Bsh1285I CGRYCG 1 cut(s) 333
BsiEI CGRYCG 1 cut(s) 333
BsiHKAI GWGCWC 1 cut(s) 284
BslFI GGGAC 1 cut(s) 60
BslI CCNNNNNNNGG 2 cut(s) 390, 427
BsmFI GGGAC 1 cut(s) 60
Bsp1286I GDGCHC 2 cut(s) 239, 284
Bsp143I GATC 2 cut(s) 4, 637
Bsp1720I GCTNAGC 1 cut(s) 440
Bsp19I CCATGG 1 cut(s) 540
BspACI CCGC 2 cut(s) 142, 333
BspCNI CTCAG 3 cut(s) 84, 432, 482
BspHI TCATGA 1 cut(s) 324
BspLI GGNNCC 3 cut(s) 22, 183, 546
BspMI ACCTGC 1 cut(s) 310
BspPI GGATC 1 cut(s) 12
BsrI ACTGG 1 cut(s) 395
BssECI CCNNGG 2 cut(s) 336, 540
BssMI GATC 2 cut(s) 4, 637
BssNI GRCGYC 2 cut(s) 225, 269
BssT1I CCWWGG 1 cut(s) 540
Bst4CI ACNGT 1 cut(s) 553
BstACI GRCGYC 2 cut(s) 225, 269
BstC8I GCNNGC 2 cut(s) 317, 446
BstDEI CTNAG 5 cut(s) 71, 240, 440, 469, 632
BstDSI CCRYGG 2 cut(s) 336, 540
BstKTI GATC 2 cut(s) 7, 640
BstMBI GATC 2 cut(s) 4, 637
BstMCI CGRYCG 1 cut(s) 333
BstMWI GCNNNNNNNGC 1 cut(s) 445
BstNSI RCATGY 1 cut(s) 535
BstSFI CTRYAG 1 cut(s) 189
BstSLI GKGCMC 1 cut(s) 239
BstV1I GCAGC 1 cut(s) 506
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BtgI CCRYGG 2 cut(s) 336, 540
BtsI GCAGTG 1 cut(s) 504
BtsIMutI CAGTG 1 cut(s) 504
BveI ACCTGC 1 cut(s) 310
Cac8I GCNNGC 2 cut(s) 317, 446
CciI TCATGA 1 cut(s) 324
Cfr13I GGNCC 1 cut(s) 544
Csp6I GTAC 3 cut(s) 196, 418, 462
CviAII CATG 5 cut(s) 173, 325, 532, 541, 645
CviJI RGCY 8 cut(s) 23, 44, 145, 188, 282, 348, 403, 448
CviKI_1 RGCY 8 cut(s) 23, 44, 145, 188, 282, 348, 403, 448
CviQI GTAC 3 cut(s) 196, 418, 462
DdeI CTNAG 5 cut(s) 71, 240, 440, 469, 632
DpnI GATC 2 cut(s) 6, 639
DpnII GATC 2 cut(s) 4, 637
DraIII CACNNNGTG 1 cut(s) 635
Ecl136II GAGCTC 1 cut(s) 282
Eco130I CCWWGG 1 cut(s) 540
Eco24I GRGCYC 1 cut(s) 284
Eco47I GGWCC 1 cut(s) 544
Eco53kI GAGCTC 1 cut(s) 282
Eco57I CTGAAG 1 cut(s) 71
EcoICRI GAGCTC 1 cut(s) 282
EcoT14I CCWWGG 1 cut(s) 540
EcoT38I GRGCYC 1 cut(s) 284
ErhI CCWWGG 1 cut(s) 540
FaeI CATG 5 cut(s) 176, 328, 535, 544, 648
FaqI GGGAC 1 cut(s) 60
FatI CATG 5 cut(s) 172, 324, 531, 540, 644
FbaI TGATCA 1 cut(s) 637
Fnu4HI GCNGC 2 cut(s) 143, 495
FriOI GRGCYC 1 cut(s) 284
Fsp4HI GCNGC 2 cut(s) 143, 495
FspBI CTAG 2 cut(s) 135, 606
GluI GCNGC 2 cut(s) 143, 495
GsaI CCCAGC 1 cut(s) 44
Hin1I GRCGYC 2 cut(s) 225, 269
Hin1II CATG 5 cut(s) 176, 328, 535, 544, 648
HincII GTYRAC 2 cut(s) 115, 529
HindII GTYRAC 2 cut(s) 115, 529
HinfI GANTC 3 cut(s) 370, 502, 626
HphI GGTGA 1 cut(s) 188
Hpy166II GTNNAC 2 cut(s) 115, 529
Hpy188I TCNGA 6 cut(s) 20, 90, 220, 369, 375, 472
Hpy188III TCNNGA 2 cut(s) 325, 489
Hpy8I GTNNAC 2 cut(s) 115, 529
Hpy99I CGWCG 4 cut(s) 20, 227, 271, 274
HpyAV CCTTC 4 cut(s) 95, 219, 367, 421
HpyCH4III ACNGT 1 cut(s) 553
HpyCH4IV ACGT 2 cut(s) 225, 269
HpyCH4V TGCA 3 cut(s) 511, 535, 589
HpyF10VI GCNNNNNNNGC 1 cut(s) 445
HpyF3I CTNAG 5 cut(s) 71, 240, 440, 469, 632
HpySE526I ACGT 2 cut(s) 225, 269
Hsp92I GRCGYC 2 cut(s) 225, 269
Hsp92II CATG 5 cut(s) 176, 328, 535, 544, 648
Ksp22I TGATCA 1 cut(s) 637
Kzo9I GATC 2 cut(s) 4, 637
LmnI GCTCC 2 cut(s) 20, 279
LpnPI CCDG 7 cut(s) 54, 198, 305, 334, 374, 376, 575
Lsp1109I GCAGC 1 cut(s) 506
MaeI CTAG 2 cut(s) 135, 606
MaeII ACGT 2 cut(s) 225, 269
MaeIII GTNAC 2 cut(s) 30, 627
MalI GATC 2 cut(s) 6, 639
MboI GATC 2 cut(s) 4, 637
MboII GAAGA 5 cut(s) 41, 59, 252, 298, 301
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 2 cut(s) 239, 284
MluCI AATT 3 cut(s) 105, 130, 410
MlyI GAGTC 3 cut(s) 379, 511, 635
MnlI CCTC 3 cut(s) 172, 338, 445
MroXI GAANNNNTTC 1 cut(s) 356
MseI TTAA 1 cut(s) 108
MwoI GCNNNNNNNGC 1 cut(s) 445
NcoI CCATGG 1 cut(s) 540
NdeII GATC 2 cut(s) 4, 637
NlaIII CATG 5 cut(s) 176, 328, 535, 544, 648
NlaIV GGNNCC 3 cut(s) 22, 183, 546
NmuCI GTSAC 1 cut(s) 627
NspI RCATGY 1 cut(s) 535
PagI TCATGA 1 cut(s) 324
PdmI GAANNNNTTC 1 cut(s) 356
PflFI GACNNNGTC 1 cut(s) 224
PkrI GCNGC 2 cut(s) 144, 496
PleI GAGTC 3 cut(s) 378, 510, 634
PpsI GAGTC 3 cut(s) 378, 510, 634
PshBI ATTAAT 1 cut(s) 108
Psp124BI GAGCTC 1 cut(s) 284
PspFI CCCAGC 1 cut(s) 40
PspN4I GGNNCC 3 cut(s) 22, 183, 546
PspPI GGNCC 1 cut(s) 544
PsuI RGATCY 1 cut(s) 4
PsyI GACNNNGTC 1 cut(s) 224
RsaI GTAC 3 cut(s) 197, 419, 463
RsaNI GTAC 3 cut(s) 196, 418, 462
SacI GAGCTC 1 cut(s) 284
SaqAI TTAA 1 cut(s) 108
SatI GCNGC 2 cut(s) 143, 495
Sau3AI GATC 2 cut(s) 4, 637
Sau96I GGNCC 1 cut(s) 544
ScaI AGTACT 2 cut(s) 197, 463
SchI GAGTC 3 cut(s) 379, 511, 635
SduI GDGCHC 2 cut(s) 239, 284
SfcI CTRYAG 1 cut(s) 189
SinI GGWCC 1 cut(s) 544
Sse9I AATT 3 cut(s) 105, 130, 410
SsiI CCGC 2 cut(s) 142, 333
SspMI CTAG 2 cut(s) 135, 606
SstI GAGCTC 1 cut(s) 284
StyI CCWWGG 1 cut(s) 540
TaaI ACNGT 1 cut(s) 553
TaiI ACGT 2 cut(s) 228, 272
TaqI TCGA 1 cut(s) 284
TasI AATT 3 cut(s) 105, 130, 410
TatI WGTACW 2 cut(s) 195, 461
TauI GCSGC 1 cut(s) 145
Tru1I TTAA 1 cut(s) 108
Tru9I TTAA 1 cut(s) 108
TscAI CASTG 1 cut(s) 504
TseFI GTSAC 1 cut(s) 627
TseI GCWGC 1 cut(s) 494
Tsp45I GTSAC 1 cut(s) 627
TspDTI ATGAA 2 cut(s) 237, 422
TspGWI ACGGA 2 cut(s) 4, 323
TspRI CASTG 1 cut(s) 504
Tth111I GACNNNGTC 1 cut(s) 224
VpaK11BI GGWCC 1 cut(s) 544
VspI ATTAAT 1 cut(s) 108
XapI RAATTY 2 cut(s) 130, 410
XceI RCATGY 1 cut(s) 535
XmnI GAANNNNTTC 1 cut(s) 356
XspI CTAG 2 cut(s) 135, 606
ZraI GACGTC 2 cut(s) 226, 270
ZrmI AGTACT 2 cut(s) 197, 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.