Rroxscaffold_2G00111720

Chaperone protein dnaJ 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0013509
Physical Location & Seq
Reverse (-)
36451383 .. 36453373
1991 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00111720.1

Sequence Viewer

Length: 465 bp
ATGGCTGCTGCTGCTGCTGGTGTGATTGGAGGAAATGGGTCTTCCTGGATTAACCAGTTTAAGGATAAGTCATCGCGGAAGAAGAAGACGGTGAACAGGGCTATGGTTTCCTGTGTTTCTTCTTCTGTGATGGATCCGTATCAGACTTTGAGGATCCGACCCGGCGCCTCTGAATCTGAGGTCAGGAAGGCTTTTAGACAGCTTGCCTTGCAGTATCATCCAGACGTATGTAGAGGAAACAATTGTGGAGTTCAGTTTCACGAAATCAATGAAGCTTATGATATTGTCATGACCAATTTGAGAGAAGAAACGACTCCATTACAGATGTATGAGACATATGATGAAGGAGAGGATGATTCGATGAGAGGAATGAATGATCCAGATTATGATATGTGGGAGGAATGGATGGGATGGGAAGGAGCCGGTATTAGGGACTACACATCTCATATCAATCCTTACATTTGA

Protein Analysis

154

Amino Acids

17.52

Weight (kDa)

4.94

Isoelectric Point (pI)

37.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 45 - 97 4.6e-15 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013509)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80920
fragaria_vesca FvH4_6g28480
malus_domestica MD09G1225800.v1.1 MD17G1224600.v1.1
prunus_persica Prupe.3G100300_v2.0.a1
pyrus_communis pycom09g14530 pycom17g22830
rosa_chinensis RchiOBHm_Chr2g0132701
rosa_laevigata RLG00000019273
rosa_multiflora Rmu_ssc0000074.1_g000011
rosa_roxburghii Rroxscaffold_2G00111720
rosa_rugosa Rorug02G0306300
rosa_samantha Rh2AG359400 Rh2BG365700 Rh2CG342500 Rh2DG382400
rosa_wichuraiana Rw2G029240 Rw3G024410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 164
AccII CGCG 1 cut(s) 76
AciI CCGC 1 cut(s) 76
AclWI GGATC 5 cut(s) 128, 141, 148, 161, 371
AcyI GRCGYC 1 cut(s) 165
AfiI CCNNNNNNNGG 2 cut(s) 61, 429
AjnI CCWGG 1 cut(s) 44
AluBI AGCT 2 cut(s) 202, 275
AluI AGCT 2 cut(s) 202, 275
Alw26I GTCTC 1 cut(s) 326
AlwI GGATC 5 cut(s) 128, 141, 148, 161, 371
ApeKI GCWGC 4 cut(s) 5, 8, 11, 14
AspLEI GCGC 1 cut(s) 167
AsuC2I CCSGG 1 cut(s) 162
AsuHPI GGTGA 1 cut(s) 103
BamHI GGATCC 2 cut(s) 133, 153
BanI GGYRCC 1 cut(s) 164
BbsI GAAGAC 2 cut(s) 33, 92
BccI CCATC 3 cut(s) 124, 400, 405
BciT130I CCWGG 1 cut(s) 46
BcnI CCSGG 1 cut(s) 162
BcoDI GTCTC 1 cut(s) 326
BfoI RGCGCY 1 cut(s) 168
BisI GCNGC 4 cut(s) 6, 9, 12, 15
BlsI GCNGC 4 cut(s) 7, 10, 13, 16
Bme1390I CCNGG 2 cut(s) 46, 162
BmiI GGNNCC 4 cut(s) 135, 155, 166, 421
BmrFI CCNGG 2 cut(s) 46, 162
BpiI GAAGAC 2 cut(s) 33, 92
BpuMI CCSGG 1 cut(s) 162
BsaBI GATNNNNATC 1 cut(s) 138
BsaHI GRCGYC 1 cut(s) 165
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 429
Bse118I RCCGGY 1 cut(s) 422
Bse1I ACTGG 1 cut(s) 55
Bse8I GATNNNNATC 1 cut(s) 138
BseBI CCWGG 1 cut(s) 46
BseGI GGATG 4 cut(s) 217, 358, 411, 416
BseJI GATNNNNATC 1 cut(s) 138
BseLI CCNNNNNNNGG 2 cut(s) 61, 429
BseMII CTCAG 1 cut(s) 168
BseNI ACTGG 1 cut(s) 55
Bsh1236I CGCG 1 cut(s) 76
BshNI GGYRCC 1 cut(s) 164
BsiSI CCGG 2 cut(s) 162, 423
BslFI GGGAC 1 cut(s) 446
BslI CCNNNNNNNGG 2 cut(s) 61, 429
BsmAI GTCTC 1 cut(s) 326
BsmFI GGGAC 1 cut(s) 446
Bsp143I GATC 3 cut(s) 133, 153, 376
BspACI CCGC 1 cut(s) 76
BspCNI CTCAG 1 cut(s) 169
BspFNI CGCG 1 cut(s) 76
BspHI TCATGA 1 cut(s) 288
BspLI GGNNCC 4 cut(s) 135, 155, 166, 421
BspPI GGATC 5 cut(s) 128, 141, 148, 161, 371
BspT107I GGYRCC 1 cut(s) 164
BsrFI RCCGGY 1 cut(s) 422
BsrI ACTGG 1 cut(s) 55
BssAI RCCGGY 1 cut(s) 422
BssMI GATC 3 cut(s) 133, 153, 376
BssNI GRCGYC 1 cut(s) 165
Bst2UI CCWGG 1 cut(s) 46
Bst4CI ACNGT 1 cut(s) 91
BstACI GRCGYC 1 cut(s) 165
BstC8I GCNNGC 1 cut(s) 204
BstDEI CTNAG 1 cut(s) 177
BstF5I GGATG 4 cut(s) 217, 358, 411, 416
BstFNI CGCG 1 cut(s) 76
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 1 cut(s) 167
BstKTI GATC 3 cut(s) 136, 156, 379
BstMAI GTCTC 1 cut(s) 326
BstMBI GATC 3 cut(s) 133, 153, 376
BstMWI GCNNNNNNNGC 3 cut(s) 11, 14, 208
BstNI CCWGG 1 cut(s) 46
BstSCI CCNGG 2 cut(s) 44, 160
BstUI CGCG 1 cut(s) 76
BstV2I GAAGAC 2 cut(s) 33, 92
BstX2I RGATCY 2 cut(s) 133, 153
BstYI RGATCY 2 cut(s) 133, 153
BtgZI GCGATG 1 cut(s) 57
BtsCI GGATG 4 cut(s) 217, 358, 411, 416
Cac8I GCNNGC 1 cut(s) 204
CciI TCATGA 1 cut(s) 288
CfoI GCGC 1 cut(s) 167
Cfr10I RCCGGY 1 cut(s) 422
CviAII CATG 1 cut(s) 289
CviJI RGCY 6 cut(s) 5, 101, 191, 202, 275, 422
CviKI_1 RGCY 6 cut(s) 5, 101, 191, 202, 275, 422
DdeI CTNAG 1 cut(s) 177
DinI GGCGCC 1 cut(s) 166
DpnI GATC 3 cut(s) 135, 155, 378
DpnII GATC 3 cut(s) 133, 153, 376
EcoRII CCWGG 1 cut(s) 44
EgeI GGCGCC 1 cut(s) 166
EheI GGCGCC 1 cut(s) 166
FaeI CATG 1 cut(s) 292
FaqI GGGAC 1 cut(s) 446
FatI CATG 1 cut(s) 288
FauNDI CATATG 1 cut(s) 337
Fnu4HI GCNGC 4 cut(s) 6, 9, 12, 15
FokI GGATG 4 cut(s) 204, 365, 418, 423
Fsp4HI GCNGC 4 cut(s) 6, 9, 12, 15
GlaI GCGC 1 cut(s) 166
GluI GCNGC 4 cut(s) 6, 9, 12, 15
HaeII RGCGCY 1 cut(s) 168
HapII CCGG 2 cut(s) 162, 423
HhaI GCGC 1 cut(s) 167
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 1 cut(s) 292
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HindIII AAGCTT 1 cut(s) 273
HinfI GANTC 3 cut(s) 173, 313, 356
HpaII CCGG 2 cut(s) 162, 423
HphI GGTGA 1 cut(s) 103
Hpy166II GTNNAC 1 cut(s) 94
Hpy188I TCNGA 4 cut(s) 144, 158, 172, 178
Hpy188III TCNNGA 5 cut(s) 184, 221, 260, 289, 380
Hpy8I GTNNAC 1 cut(s) 94
HpyAV CCTTC 3 cut(s) 181, 338, 410
HpyCH4III ACNGT 1 cut(s) 91
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 1 cut(s) 211
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 14, 208
HpyF3I CTNAG 1 cut(s) 177
HpySE526I ACGT 1 cut(s) 225
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 1 cut(s) 292
HspAI GCGC 1 cut(s) 165
KasI GGCGCC 1 cut(s) 164
Kzo9I GATC 3 cut(s) 133, 153, 376
LmnI GCTCC 1 cut(s) 419
MaeII ACGT 1 cut(s) 225
MalI GATC 3 cut(s) 135, 155, 378
MboI GATC 3 cut(s) 133, 153, 376
MboII GAAGA 7 cut(s) 33, 91, 94, 97, 111, 114, 317
MfeI CAATTG 1 cut(s) 241
MflI RGATCY 2 cut(s) 133, 153
MluCI AATT 2 cut(s) 241, 295
Mly113I GGCGCC 1 cut(s) 165
MlyI GAGTC 1 cut(s) 307
MmeI TCCRAC 1 cut(s) 181
MnlI CCTC 8 cut(s) 23, 144, 172, 178, 227, 343, 359, 391
MseI TTAA 2 cut(s) 51, 60
MspI CCGG 2 cut(s) 162, 423
MspR9I CCNGG 2 cut(s) 46, 162
MunI CAATTG 1 cut(s) 241
MvaI CCWGG 1 cut(s) 46
MvnI CGCG 1 cut(s) 76
MwoI GCNNNNNNNGC 3 cut(s) 11, 14, 208
NarI GGCGCC 1 cut(s) 165
NciI CCSGG 1 cut(s) 162
NdeI CATATG 1 cut(s) 337
NdeII GATC 3 cut(s) 133, 153, 376
NlaIII CATG 1 cut(s) 292
NlaIV GGNNCC 4 cut(s) 135, 155, 166, 421
PagI TCATGA 1 cut(s) 288
PfeI GAWTC 2 cut(s) 173, 356
PfoI TCCNGGA 1 cut(s) 44
PkrI GCNGC 4 cut(s) 7, 10, 13, 16
PleI GAGTC 1 cut(s) 307
PluTI GGCGCC 1 cut(s) 168
PpsI GAGTC 1 cut(s) 307
Psp6I CCWGG 1 cut(s) 44
PspGI CCWGG 1 cut(s) 44
PspN4I GGNNCC 4 cut(s) 135, 155, 166, 421
PsuI RGATCY 2 cut(s) 133, 153
SaqAI TTAA 2 cut(s) 51, 60
SatI GCNGC 4 cut(s) 6, 9, 12, 15
Sau3AI GATC 3 cut(s) 133, 153, 376
SchI GAGTC 1 cut(s) 307
ScrFI CCNGG 2 cut(s) 46, 162
SetI ASST 4 cut(s) 183, 204, 228, 277
SfoI GGCGCC 1 cut(s) 166
Sse9I AATT 2 cut(s) 241, 295
SsiI CCGC 1 cut(s) 76
SspDI GGCGCC 1 cut(s) 164
StyD4I CCNGG 2 cut(s) 44, 160
TaaI ACNGT 1 cut(s) 91
TaiI ACGT 1 cut(s) 228
TaqI TCGA 1 cut(s) 359
TasI AATT 2 cut(s) 241, 295
TfiI GAWTC 2 cut(s) 173, 356
Tru1I TTAA 2 cut(s) 51, 60
Tru9I TTAA 2 cut(s) 51, 60
TseI GCWGC 4 cut(s) 5, 8, 11, 14
TspDTI ATGAA 3 cut(s) 285, 357, 386
TspGWI ACGGA 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.