MD17G1224600.v1.1

Chaperone protein dnaJ 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0013509
Physical Location & Seq
Reverse (-)
27298682 .. 27301266
2585 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1224600.v1.1.491

Sequence Viewer

Length: 480 bp
ATGGCTTGCGTTGGAGCTGGTTTGATCGGGGGCAGTGGGTCGTCCTGGAATCTGTTGAAGGAAAATAAGGCGAGGAGGAAGAAGATGGTGAATGATAAGAGGGGGATGGTTTCTTGTGTTGCTTCATCTTCTGTGATGGATCCTTACCGGACCTTGAAGATTCAACCTGGTGCCTCTGAATCTGAGGTCAGGAAGGCTTTTAGACAACTTGCCTTGAAGTATCATCCAGATGTATGCAGAGGAAACAATTGTAGAGTTCAGTTTCACCAGATCAACGAAGCCTATGATATTGTTATGAGCGATTTGAGAGGAGAAACGACGACGACGACTCCATCGCAGATGTATGAGACGTACGACGAAGGGGATGACGAGCCGATGAGGGGAATGAACGATCCAGATTGGGACATGTGGGAGGAATGGATGGGATGGGAAGGAGCTGGAATTAGGGACTACACATCTCACATTAATCCTTATATTTGA

Protein Analysis

160

Amino Acids

18.07

Weight (kDa)

5.4

Isoelectric Point (pI)

40.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 47 - 99 1.9e-15 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013509)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80920
fragaria_vesca FvH4_6g28480
malus_domestica MD09G1225800.v1.1 MD17G1224600.v1.1
prunus_persica Prupe.3G100300_v2.0.a1
pyrus_communis pycom09g14530 pycom17g22830
rosa_chinensis RchiOBHm_Chr2g0132701
rosa_laevigata RLG00000019273
rosa_multiflora Rmu_ssc0000074.1_g000011
rosa_roxburghii Rroxscaffold_2G00111720
rosa_rugosa Rorug02G0306300
rosa_samantha Rh2AG359400 Rh2BG365700 Rh2CG342500 Rh2DG382400
rosa_wichuraiana Rw2G029240 Rw3G024410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 170
AclWI GGATC 3 cut(s) 134, 147, 386
AfaI GTAC 1 cut(s) 353
AfiI CCNNNNNNNGG 1 cut(s) 380
AflIII ACRYGT 1 cut(s) 405
AgsI TTSAA 4 cut(s) 58, 157, 164, 217
AjnI CCWGG 2 cut(s) 44, 166
AluBI AGCT 2 cut(s) 17, 437
AluI AGCT 2 cut(s) 17, 437
Alw26I GTCTC 1 cut(s) 341
AlwI GGATC 3 cut(s) 134, 147, 386
AseI ATTAAT 1 cut(s) 465
AspS9I GGNCC 1 cut(s) 150
AsuHPI GGTGA 2 cut(s) 100, 257
AvaII GGWCC 1 cut(s) 150
BamHI GGATCC 1 cut(s) 139
BanI GGYRCC 1 cut(s) 170
BccI CCATC 6 cut(s) 79, 100, 130, 340, 415, 420
BciT130I CCWGG 2 cut(s) 46, 168
BcoDI GTCTC 1 cut(s) 341
Bme1390I CCNGG 2 cut(s) 46, 168
Bme18I GGWCC 1 cut(s) 150
BmgT120I GGNCC 1 cut(s) 150
BmiI GGNNCC 2 cut(s) 141, 172
BmrFI CCNGG 2 cut(s) 46, 168
BsaWI WCCGGW 1 cut(s) 147
BsaXI ACNNNNNCTCC 2 cut(s) 313, 343
Bsc4I CCNNNNNNNGG 1 cut(s) 380
BseBI CCWGG 2 cut(s) 46, 168
BseGI GGATG 5 cut(s) 111, 223, 370, 426, 431
BseLI CCNNNNNNNGG 1 cut(s) 380
BseMII CTCAG 1 cut(s) 174
BseRI GAGGAG 2 cut(s) 88, 324
BshNI GGYRCC 1 cut(s) 170
BsiSI CCGG 1 cut(s) 148
BsiWI CGTACG 1 cut(s) 351
BslFI GGGAC 2 cut(s) 416, 461
BslI CCNNNNNNNGG 1 cut(s) 380
BsmAI GTCTC 1 cut(s) 341
BsmBI CGTCTC 1 cut(s) 341
BsmFI GGGAC 2 cut(s) 416, 461
Bsp143I GATC 4 cut(s) 24, 139, 270, 391
BspCNI CTCAG 1 cut(s) 175
BspLI GGNNCC 2 cut(s) 141, 172
BspPI GGATC 3 cut(s) 134, 147, 386
BspT107I GGYRCC 1 cut(s) 170
BssMI GATC 4 cut(s) 24, 139, 270, 391
Bst2UI CCWGG 2 cut(s) 46, 168
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 1 cut(s) 183
BstF5I GGATG 5 cut(s) 111, 223, 370, 426, 431
BstKTI GATC 4 cut(s) 27, 142, 273, 394
BstMAI GTCTC 1 cut(s) 341
BstMBI GATC 4 cut(s) 24, 139, 270, 391
BstNI CCWGG 2 cut(s) 46, 168
BstNSI RCATGY 1 cut(s) 409
BstSCI CCNGG 2 cut(s) 44, 166
BstX2I RGATCY 1 cut(s) 139
BstYI RGATCY 1 cut(s) 139
BtgZI GCGATG 1 cut(s) 318
BtsCI GGATG 5 cut(s) 111, 223, 370, 426, 431
BtsI GCAGTG 1 cut(s) 40
BtsIMutI CAGTG 1 cut(s) 40
Cac8I GCNNGC 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 150
CsiI ACCWGGT 1 cut(s) 166
Csp6I GTAC 1 cut(s) 352
CviAII CATG 1 cut(s) 406
CviJI RGCY 6 cut(s) 5, 17, 197, 281, 373, 437
CviKI_1 RGCY 6 cut(s) 5, 17, 197, 281, 373, 437
CviQI GTAC 1 cut(s) 352
DdeI CTNAG 1 cut(s) 183
DpnI GATC 4 cut(s) 26, 141, 272, 393
DpnII GATC 4 cut(s) 24, 139, 270, 391
Eco47I GGWCC 1 cut(s) 150
EcoRII CCWGG 2 cut(s) 44, 166
Esp3I CGTCTC 1 cut(s) 341
FaeI CATG 1 cut(s) 409
FaiI YATR 6 cut(s) 235, 285, 296, 345, 407, 474
FaqI GGGAC 2 cut(s) 416, 461
FatI CATG 1 cut(s) 405
FokI GGATG 5 cut(s) 118, 210, 377, 433, 438
HapII CCGG 1 cut(s) 148
Hin1II CATG 1 cut(s) 409
HinfI GANTC 4 cut(s) 49, 160, 179, 328
HpaII CCGG 1 cut(s) 148
HphI GGTGA 2 cut(s) 100, 257
Hpy188I TCNGA 2 cut(s) 178, 184
Hpy188III TCNNGA 3 cut(s) 190, 227, 395
Hpy99I CGWCG 4 cut(s) 322, 325, 328, 359
HpyAV CCTTC 4 cut(s) 52, 187, 353, 425
HpyCH4IV ACGT 1 cut(s) 350
HpyCH4V TGCA 1 cut(s) 237
HpyF3I CTNAG 1 cut(s) 183
HpySE526I ACGT 1 cut(s) 350
Hsp92II CATG 1 cut(s) 409
Kzo9I GATC 4 cut(s) 24, 139, 270, 391
LmnI GCTCC 2 cut(s) 14, 434
MabI ACCWGGT 1 cut(s) 166
MaeII ACGT 1 cut(s) 350
MalI GATC 4 cut(s) 26, 141, 272, 393
MboI GATC 4 cut(s) 24, 139, 270, 391
MboII GAAGA 4 cut(s) 91, 94, 120, 169
MfeI CAATTG 1 cut(s) 247
MflI RGATCY 1 cut(s) 139
MluCI AATT 2 cut(s) 247, 441
MlyI GAGTC 1 cut(s) 322
MnlI CCTC 9 cut(s) 66, 69, 93, 178, 184, 233, 302, 372, 406
MseI TTAA 1 cut(s) 465
MslI CAYNNNNRTG 1 cut(s) 228
MspI CCGG 1 cut(s) 148
MspR9I CCNGG 2 cut(s) 46, 168
MunI CAATTG 1 cut(s) 247
MvaI CCWGG 2 cut(s) 46, 168
NdeII GATC 4 cut(s) 24, 139, 270, 391
NlaIII CATG 1 cut(s) 409
NlaIV GGNNCC 2 cut(s) 141, 172
NspI RCATGY 1 cut(s) 409
PciI ACATGT 1 cut(s) 405
PcsI WCGNNNNNNNCGW 1 cut(s) 323
PfeI GAWTC 3 cut(s) 49, 160, 179
Pfl23II CGTACG 1 cut(s) 351
PfoI TCCNGGA 1 cut(s) 44
PleI GAGTC 1 cut(s) 322
PpsI GAGTC 1 cut(s) 322
PscI ACATGT 1 cut(s) 405
PshBI ATTAAT 1 cut(s) 465
Psp6I CCWGG 2 cut(s) 44, 166
PspGI CCWGG 2 cut(s) 44, 166
PspLI CGTACG 1 cut(s) 351
PspN4I GGNNCC 2 cut(s) 141, 172
PspPI GGNCC 1 cut(s) 150
PsuI RGATCY 1 cut(s) 139
RsaI GTAC 1 cut(s) 353
RsaNI GTAC 1 cut(s) 352
RseI CAYNNNNRTG 1 cut(s) 228
SaqAI TTAA 1 cut(s) 465
Sau3AI GATC 4 cut(s) 24, 139, 270, 391
Sau96I GGNCC 1 cut(s) 150
SchI GAGTC 1 cut(s) 322
ScrFI CCNGG 2 cut(s) 46, 168
SetI ASST 6 cut(s) 19, 155, 169, 189, 353, 439
SexAI ACCWGGT 1 cut(s) 166
SinI GGWCC 1 cut(s) 150
SmiMI CAYNNNNRTG 1 cut(s) 228
Sse9I AATT 2 cut(s) 247, 441
StyD4I CCNGG 2 cut(s) 44, 166
TaiI ACGT 1 cut(s) 353
TasI AATT 2 cut(s) 247, 441
TfiI GAWTC 3 cut(s) 49, 160, 179
Tru1I TTAA 1 cut(s) 465
Tru9I TTAA 1 cut(s) 465
TscAI CASTG 1 cut(s) 40
TspDTI ATGAA 2 cut(s) 114, 401
TspRI CASTG 1 cut(s) 40
VpaK11BI GGWCC 1 cut(s) 150
VspI ATTAAT 1 cut(s) 465
XceI RCATGY 1 cut(s) 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.