Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0005588

Physical Location & Seq

Genomic Coordinates Forward (+)

29478796 .. 29482454

Gene Structure 3659 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1234500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2106 bp

ATGTCACCTGATTGCTTTTTAATGGCAGCATCATCAGCATGCCTTGTGGGGAATGGGTTATCTACCCACGGTACTAAGCCAAATTTGAGCAAGGACTTCTGTGGCAGGCATCTCTTGCACCCTTCAGCTCTTCCATCGTTGGGTAAAGTGACAAAAACAGTTCTCACAAAGGCGTCGCTAGACCAAAGGCAAAACGAGGGAAGAAGAGGCTTTCTCAAGTTGTTGCTTGGGAATGTGGGAATAGGAGCACCAGCTATACTTGGAGCGGGGAATGCTTTGGCCGCCGATGATCAAGGTGTCTCTTCTTCAAGGATGTCTTATTCCAGGTTCTTGGAGTATCTGGACAAGGGCAGGGTGACTAAAGTTGATTTGTTTGAGAATGGAACCATTGCTATTGTTGAGGCTGTTTCTCCTGAGTTAGGGAACCGATTGCAGCGGGTTCGTGTTCAACTTCCGGGGCTAAGCCAAGAGCTCCTCCAGAAGTTCAGGGAAAAGAACATCGACTTTGCAGCACATAATGCTCAAGAAGACTCGGGCTCTCTTTTATTTAACCTGATTGGAAATCTTGCTTTCCCACTAATTTTAATTGGGGGTTTGTTCCTACTCTCAAGGCGTTCATCCGGGGGCATGGGTGGTCCAGGTGGGCCTGGATTTCCTCTACAATTTGGTCAATCTAAAGCCAAGTTCCAAATGGAACCAAACACCGGAGTGACATTTGATGATGTTGCTGGGGTAGATGAAGCCAAGCAAGATTTCGTGGAGGTGGTCGAGTTCTTAAAGAAGCCTGAGAGGTTCACTGCTGTTGGGGCTCGAATTCCTAAAGGTGTTCTTCTTGTTGGCCCTCCGGGAACCGGAAAGACTCTGCTAGCAAAGGCAATTGCTGGTGAAGCTGGTGTTCCATTTTTCTCCATCTCAGGTTCTGAGTTTGTTGAAATGTTTGTTGGTGTTGGTGCTTCCCGAGTTCGTGATCTTTTTAAGAAGGCCAAGGAGAATGCACCTTGCATTGTATTTGTTGATGAAATTGACGCTGTTGGACGGCAAAGAGGAACTGGAATTGGTGGAGGGAACGATGAAAGGGAACAAACCCTTAATCAGCTTTTGACAGAAATGGATGGTTTTGAGGGCAACACTGGTATCATTGTGGTTGCAGCGACTAACAGGGCAGATATTCTTGACTCTGCCTTACTGAGGCCTGGCCGGTTTGACAGGCAGGTGTCTGTTGATGTCCCAGACATTCGAGGAAGAACAGAGATCTTAAAGGTTCATGCTAGCAATAAGAAGTTTGATGGAAGTGTTTCCCTTGATGTTGTAGCCATGAGGACACCCGGCTTCAGTGGGGCTGATCTCGCAAACCTCCTTAATGAAGCAGCTATATTGGCTGGTCGACGTGGGAAGTCAGCAATTTCATCTAAAGAGATTGATGATTCAATTGATAGAATAGTGGCTGGAATGGAAGGAACAGTAATGACAGATGGAAAGAGTAAAAGTCTGGTGGCATATCACGAAGTTGGGCATGCCATCTGTGGGACATTGACTCCAGGGCATGATGCTGTTCAAAAGGTGACATTAGTCCCTCGTGGTCAGGCTCGGGGTCTTACATGGTTTATTCCTGCAGATGATCCTACCTTGATCTCCAAGCAGCAACTGTTTGCAAGAATTGTTGGTGGACTTGGTGGTAGAGCCGCAGAGGAAGTGATCTTTGGCGAGCCTGAGGTGACAACAGGTGCAGCTGGTGATTTGCAGCAGATCACTGGTTTGGCCAAACAGATGGTCACCACCTTCGGAATGTCTGATATTGGACCATGGTCATTGATGGATGCGTCCCAGAGCGGTGACGTCATTATGAGAATGATGGCAAGGAACTCAATGTCAGAAAAGCTTGCTGAAGACATTGATACAGCTATTAAGAGATTATCGGATGAAGCATATGAGATTGCGTTGACCCACATAAGAAACAACCGGGAAGCCATTGACAAGATCGTGGAGGTCCTTCTTGAAAAGGAAACAATGACCGGGGATGAATTCCGGGCACTCCTATCCGAATTCGCTGAAATCCCAGTTGAAAACCGGGTCCCTGCTGCAGTTCCGTCGCCTGTAGCTGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004176 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006091 GO:0006508 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009056 GO:0009057 GO:0009314 GO:0009416 GO:0009507 GO:0009526 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009628 GO:0009642 GO:0009644 GO:0009657 GO:0009658 GO:0009668 GO:0009719 GO:0009725 GO:0009735 GO:0009765 GO:0009892 GO:0009941 GO:0009987 GO:0010027 GO:0010033 GO:0010109 GO:0010205 GO:0010206 GO:0010304 GO:0015979 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0019538 GO:0019684 GO:0022607 GO:0030091 GO:0030163 GO:0031323 GO:0031324 GO:0031967 GO:0031975 GO:0031976 GO:0031977 GO:0031984 GO:0034357 GO:0034622 GO:0042221 GO:0042548 GO:0042623 GO:0042651 GO:0043155 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043467 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0048519 GO:0048523 GO:0048564 GO:0050789 GO:0050794 GO:0050896 GO:0055035 GO:0061024 GO:0065003 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072593 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1905156

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA FtsH_ext Peptidase_M41

Protein Analysis

702

Amino Acids

74.71

Weight (kDa)

5.71

Isoelectric Point (pI)

32.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FtsH_ext	PF06480	96 - 171	2.1e-07	FtsH Extracellular
AAA	PF00004	276 - 408	4.1e-46	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	431 - 474	2e-12	AAA+ lid domain
Peptidase_M41	PF01434	490 - 677	2.2e-67	Peptidase family M41

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005588)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06430 AT1G06430 AT1G06430 AT2G30950 AT2G30950 AT2G30950 AT2G30950
fragaria_vesca	FvH4_6g27780 FvH4_6g27780 FvH4_6g27780
malus_domestica	MD09G1234500.v1.1 MD17G1263600.v1.1
prunus_persica	Prupe.3G112700_v2.0.a1 Prupe.3G112700_v2.0.a1
pyrus_communis	pycom09g15280 pycom17g26380
rosa_chinensis	RchiOBHm_Chr2g0131061
rosa_roxburghii	Rroxscaffold_2G00113260
rosa_samantha	Rh2AG347000 Rh2AG479700 Rh2BG354500 Rh2BG461700 Rh2CG333000 Rh2DG372500 Rh2DG470900
rosa_wichuraiana	Rw2G028140 Rw2G028180 Rw4G004930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1201
AatII	GACGTC	1 cut(s)	1839
Acc36I	ACCTGC	1 cut(s)	1201
AccBSI	CCGCTC	2 cut(s)	266, 1830
AccI	GTMKAC	1 cut(s)	1384
AciI	CCGC	5 cut(s)	266, 282, 436, 1683, 1830
AclWI	GGATC	1 cut(s)	1613
AcoI	YGGCCR	3 cut(s)	279, 1195, 1758
AcsI	RAATTY	4 cut(s)	82, 813, 2021, 2042
AcuI	CTGAAG	3 cut(s)	108, 1315, 1905
AcyI	GRCGYC	2 cut(s)	173, 1836
AfaI	GTAC	1 cut(s)	73
AfiI	CCNNNNNNNGG	6 cut(s)	140, 419, 704, 851, 1188, 1524
AgsI	TTSAA	7 cut(s)	309, 449, 932, 1428, 1556, 1997, 2063
AjiI	CACGTC	1 cut(s)	1388
AjnI	CCWGG	5 cut(s)	323, 637, 646, 1192, 1537
AjuI	GAANNNNNNNTTGG	6 cut(s)	737, 769, 924, 956, 1683, 1715
AleI	CACNNNNGTG	1 cut(s)	707
Alw21I	GWGCWC	2 cut(s)	250, 474
Alw26I	GTCTC	1 cut(s)	304
AlwI	GGATC	1 cut(s)	1613
AlwNI	CAGNNNCTG	2 cut(s)	920, 1645
Ama87I	CYCGRG	3 cut(s)	532, 957, 1587
AoxI	GGCC	7 cut(s)	279, 644, 838, 981, 1190, 1195, 1758
ApeKI	GCWGC	9 cut(s)	26, 433, 509, 1148, 1367, 1639, 1727, 1741, 2078
ApoI	RAATTY	4 cut(s)	82, 813, 2021, 2042
ArsI	GACNNNNNNTTYG	2 cut(s)	1755, 1787
Asp700I	GAANNNNTTC	1 cut(s)	1294
AspS9I	GGNCC	6 cut(s)	635, 644, 839, 1799, 1987, 2071
AsuC2I	CCSGG	8 cut(s)	456, 622, 846, 1326, 1961, 2014, 2027, 2069
AsuHPI	GGTGA	7 cut(s)	367, 896, 1573, 1726, 1745, 1765, 1844
AsuNHI	GCTAGC	2 cut(s)	865, 1268
AvaI	CYCGRG	3 cut(s)	532, 957, 1587
AvaII	GGWCC	4 cut(s)	635, 1799, 1987, 2071
AxyI	CCTNAGG	1 cut(s)	1710
BaeGI	GKGCMC	1 cut(s)	2032
BaeI	ACNNNNGTAYC	2 cut(s)	1117, 1150
BalI	TGGCCA	1 cut(s)	1760
BanII	GRGCYC	3 cut(s)	474, 539, 811
BauI	CACGAG	1 cut(s)	1575
BbsI	GAAGAC	2 cut(s)	534, 1893
Bbv12I	GWGCWC	2 cut(s)	250, 474
BbvI	GCAGC	9 cut(s)	38, 445, 521, 1160, 1379, 1651, 1739, 1753, 2065
BccI	CCATC	9 cut(s)	142, 917, 1106, 1280, 1466, 1526, 1762, 1807, 1846
BceAI	ACGGC	1 cut(s)	1052
BcgI	CGANNNNNNTGC	4 cut(s)	422, 456, 2070, 2104
BciT130I	CCWGG	5 cut(s)	325, 639, 648, 1194, 1539
BclI	TGATCA	1 cut(s)	289
BcnI	CCSGG	8 cut(s)	456, 622, 846, 1326, 1961, 2014, 2027, 2069
BcoDI	GTCTC	1 cut(s)	304
BfaI	CTAG	3 cut(s)	179, 866, 1269
BfmI	CTRYAG	3 cut(s)	1611, 2079, 2094
BfuAI	ACCTGC	1 cut(s)	1201
BglII	AGATCT	1 cut(s)	1251
BlpI	GCTNAGC	1 cut(s)	461
Bme18I	GGWCC	4 cut(s)	635, 1799, 1987, 2071
BmeT110I	CYCGRG	3 cut(s)	532, 957, 1587
BmgBI	CACGTC	1 cut(s)	1388
BmgT120I	GGNCC	6 cut(s)	635, 644, 839, 1799, 1987, 2071
BmiI	GGNNCC	6 cut(s)	385, 425, 696, 850, 2072, 2073
BmrI	ACTGGG	1 cut(s)	2051
BmsI	GCATC	4 cut(s)	38, 118, 1537, 1807
BmtI	GCTAGC	2 cut(s)	869, 1272
BmuI	ACTGGG	1 cut(s)	2051
BoxI	GACNNNNGTC	2 cut(s)	1568, 1804
BpiI	GAAGAC	2 cut(s)	534, 1893
BplI	GAGNNNNNCTC	2 cut(s)	198, 230
BpmI	CTGGAG	2 cut(s)	461, 1521
Bpu1102I	GCTNAGC	1 cut(s)	461
BpuEI	CTTGAG	3 cut(s)	200, 507, 592
BpuMI	CCSGG	8 cut(s)	456, 622, 846, 1326, 1961, 2014, 2027, 2069
BsaHI	GRCGYC	2 cut(s)	173, 1836
BsaJI	CCNNGG	7 cut(s)	67, 455, 621, 984, 1538, 1802, 2013
BsaWI	WCCGGW	2 cut(s)	704, 851
BsaXI	ACNNNNNCTCC	2 cut(s)	1519, 1549
Bsc4I	CCNNNNNNNGG	6 cut(s)	140, 419, 704, 851, 1188, 1524
Bse118I	RCCGGY	1 cut(s)	1197
Bse1I	ACTGG	4 cut(s)	1054, 1135, 1756, 2057
Bse21I	CCTNAGG	1 cut(s)	1710
Bse3DI	GCAATG	1 cut(s)	387
BseBI	CCWGG	5 cut(s)	325, 639, 648, 1194, 1539
BseDI	CCNNGG	7 cut(s)	67, 455, 621, 984, 1538, 1802, 2013
BseGI	GGATG	6 cut(s)	318, 617, 1117, 1822, 1924, 2023
BseLI	CCNNNNNNNGG	6 cut(s)	140, 419, 704, 851, 1188, 1524
BseMI	GCAATG	1 cut(s)	387
BseMII	CTCAG	6 cut(s)	405, 777, 912, 927, 1178, 1701
BseNI	ACTGG	4 cut(s)	1054, 1135, 1756, 2057
BseRI	GAGGAG	1 cut(s)	464
BseSI	GKGCMC	1 cut(s)	2032
BseXI	GCAGC	9 cut(s)	38, 445, 521, 1160, 1379, 1651, 1739, 1753, 2065
BseYI	CCCAGC	1 cut(s)	728
BsgI	GTGCAG	1 cut(s)	1746
BshFI	GGCC	7 cut(s)	281, 646, 840, 983, 1192, 1197, 1760
BsiHKAI	GWGCWC	2 cut(s)	250, 474
BsiHKCI	CYCGRG	3 cut(s)	532, 957, 1587
BslFI	GGGAC	5 cut(s)	1211, 1540, 1556, 1807, 2057
BslI	CCNNNNNNNGG	6 cut(s)	140, 419, 704, 851, 1188, 1524
BsmAI	GTCTC	1 cut(s)	304
BsmFI	GGGAC	5 cut(s)	1211, 1540, 1556, 1807, 2057
BsmI	GAATGC	2 cut(s)	277, 997
BsnI	GGCC	7 cut(s)	281, 646, 840, 983, 1192, 1197, 1760
BsoBI	CYCGRG	3 cut(s)	532, 957, 1587
Bsp1286I	GDGCHC	5 cut(s)	250, 474, 539, 811, 2032
Bsp143I	GATC	9 cut(s)	289, 967, 1251, 1342, 1618, 1629, 1695, 1746, 1977
Bsp1720I	GCTNAGC	1 cut(s)	461
Bsp19I	CCATGG	1 cut(s)	1802
BspACI	CCGC	5 cut(s)	266, 282, 436, 1683, 1830
BspANI	GGCC	7 cut(s)	281, 646, 840, 983, 1192, 1197, 1760
BspCNI	CTCAG	6 cut(s)	406, 778, 913, 926, 1179, 1702
BspLI	GGNNCC	6 cut(s)	385, 425, 696, 850, 2072, 2073
BspMAI	CTGCAG	2 cut(s)	1615, 2083
BspMI	ACCTGC	1 cut(s)	1201
BspOI	GCTAGC	2 cut(s)	869, 1272
BspPI	GGATC	1 cut(s)	1613
BspQI	GCTCTTC	1 cut(s)	135
BsrBI	CCGCTC	2 cut(s)	266, 1830
BsrDI	GCAATG	1 cut(s)	387
BsrFI	RCCGGY	1 cut(s)	1197
BsrI	ACTGG	4 cut(s)	1054, 1135, 1756, 2057
BssAI	RCCGGY	1 cut(s)	1197
BssECI	CCNNGG	7 cut(s)	67, 455, 621, 984, 1538, 1802, 2013
BssMI	GATC	9 cut(s)	289, 967, 1251, 1342, 1618, 1629, 1695, 1746, 1977
BssNI	GRCGYC	2 cut(s)	173, 1836
BssSI	CACGAG	1 cut(s)	1575
BssT1I	CCWWGG	2 cut(s)	984, 1802
Bst2BI	CACGAG	1 cut(s)	1575
Bst2UI	CCWGG	5 cut(s)	325, 639, 648, 1194, 1539
Bst4CI	ACNGT	4 cut(s)	71, 160, 1462, 1647
Bst6I	CTCTTC	3 cut(s)	135, 199, 307
BstACI	GRCGYC	2 cut(s)	173, 1836
BstAPI	GCANNNNNTGC	2 cut(s)	115, 518
BstC8I	GCNNGC	7 cut(s)	40, 107, 867, 1270, 1515, 1706, 1881
BstDEI	CTNAG	8 cut(s)	75, 414, 461, 786, 913, 921, 1187, 1710
BstDSI	CCRYGG	2 cut(s)	67, 1802
BstEII	GGTNACC	1 cut(s)	1771
BstENI	CCTNNNNNAGG	2 cut(s)	417, 1186
BstF5I	GGATG	6 cut(s)	318, 617, 1117, 1822, 1924, 2023
BstKTI	GATC	9 cut(s)	292, 970, 1254, 1345, 1621, 1632, 1698, 1749, 1980
BstMAI	GTCTC	1 cut(s)	304
BstMBI	GATC	9 cut(s)	289, 967, 1251, 1342, 1618, 1629, 1695, 1746, 1977
BstMWI	GCNNNNNNNGC	9 cut(s)	35, 115, 272, 281, 518, 806, 887, 1346, 1376
BstNI	CCWGG	5 cut(s)	325, 639, 648, 1194, 1539
BstNSI	RCATGY	2 cut(s)	42, 1517
BstPAI	GACNNNNGTC	2 cut(s)	1568, 1804
BstPI	GGTNACC	1 cut(s)	1771
BstSFI	CTRYAG	3 cut(s)	1611, 2079, 2094
BstSLI	GKGCMC	1 cut(s)	2032
BstV1I	GCAGC	9 cut(s)	38, 445, 521, 1160, 1379, 1651, 1739, 1753, 2065
BstV2I	GAAGAC	2 cut(s)	534, 1893
BstX2I	RGATCY	1 cut(s)	1251
BstXI	CCANNNNNNTGG	2 cut(s)	331, 1768
BstYI	RGATCY	1 cut(s)	1251
Bsu36I	CCTNAGG	1 cut(s)	1710
BsuRI	GGCC	7 cut(s)	281, 646, 840, 983, 1192, 1197, 1760
BtgI	CCRYGG	2 cut(s)	67, 1802
BtrI	CACGTC	1 cut(s)	1388
BtsCI	GGATG	6 cut(s)	318, 617, 1117, 1822, 1924, 2023
BtsI	GCAGTG	1 cut(s)	795
BtsIMutI	CAGTG	4 cut(s)	795, 1128, 1339, 1749
BveI	ACCTGC	1 cut(s)	1201
Cac8I	GCNNGC	7 cut(s)	40, 107, 867, 1270, 1515, 1706, 1881
CaiI	CAGNNNCTG	2 cut(s)	920, 1645
Cfr10I	RCCGGY	1 cut(s)	1197
Cfr13I	GGNCC	6 cut(s)	635, 644, 839, 1799, 1987, 2071
CseI	GACGC	3 cut(s)	162, 1034, 1809
Csp6I	GTAC	1 cut(s)	72
CviAII	CATG	8 cut(s)	39, 628, 1265, 1315, 1514, 1544, 1599, 1803
CviQI	GTAC	1 cut(s)	72
DdeI	CTNAG	8 cut(s)	75, 414, 461, 786, 913, 921, 1187, 1710
DpnI	GATC	9 cut(s)	291, 969, 1253, 1344, 1620, 1631, 1697, 1748, 1979
DpnII	GATC	9 cut(s)	289, 967, 1251, 1342, 1618, 1629, 1695, 1746, 1977
EaeI	YGGCCR	3 cut(s)	279, 1195, 1758
Eam1104I	CTCTTC	3 cut(s)	135, 199, 307
EarI	CTCTTC	3 cut(s)	135, 199, 307
Ecl136II	GAGCTC	1 cut(s)	472
Eco130I	CCWWGG	2 cut(s)	984, 1802
Eco147I	AGGCCT	1 cut(s)	1192
Eco24I	GRGCYC	3 cut(s)	474, 539, 811
Eco47I	GGWCC	4 cut(s)	635, 1799, 1987, 2071
Eco53kI	GAGCTC	1 cut(s)	472
Eco57I	CTGAAG	3 cut(s)	108, 1315, 1905
Eco81I	CCTNAGG	1 cut(s)	1710
Eco88I	CYCGRG	3 cut(s)	532, 957, 1587
Eco91I	GGTNACC	1 cut(s)	1771
EcoICRI	GAGCTC	1 cut(s)	472
EcoNI	CCTNNNNNAGG	2 cut(s)	417, 1186
EcoO109I	RGGNCCY	2 cut(s)	1987, 2071
EcoO65I	GGTNACC	1 cut(s)	1771
EcoRI	GAATTC	3 cut(s)	813, 2021, 2042
EcoRII	CCWGG	5 cut(s)	323, 637, 646, 1192, 1537
EcoT14I	CCWWGG	2 cut(s)	984, 1802
EcoT38I	GRGCYC	3 cut(s)	474, 539, 811
ErhI	CCWWGG	2 cut(s)	984, 1802
FaeI	CATG	8 cut(s)	42, 631, 1268, 1318, 1517, 1547, 1602, 1806
FalI	AAGNNNNNCTT	4 cut(s)	301, 333, 813, 845
FaqI	GGGAC	5 cut(s)	1211, 1540, 1556, 1807, 2057
FatI	CATG	8 cut(s)	38, 627, 1264, 1314, 1513, 1543, 1598, 1802
FauI	CCCGC	2 cut(s)	259, 429
FauNDI	CATATG	1 cut(s)	1927
FbaI	TGATCA	1 cut(s)	289
FblI	GTMKAC	1 cut(s)	1384
FokI	GGATG	6 cut(s)	325, 604, 1124, 1829, 1931, 2030
FriOI	GRGCYC	3 cut(s)	474, 539, 811
FspBI	CTAG	3 cut(s)	179, 866, 1269
GsaI	CCCAGC	1 cut(s)	732
GsuI	CTGGAG	2 cut(s)	461, 1521
HaeIII	GGCC	7 cut(s)	281, 646, 840, 983, 1192, 1197, 1760
HgaI	GACGC	3 cut(s)	162, 1034, 1809
Hin1I	GRCGYC	2 cut(s)	173, 1836
Hin1II	CATG	8 cut(s)	42, 631, 1268, 1318, 1517, 1547, 1602, 1806
HincII	GTYRAC	2 cut(s)	1385, 1941
HindII	GTYRAC	2 cut(s)	1385, 1941
HindIII	AAGCTT	1 cut(s)	1877
HinfI	GANTC	5 cut(s)	530, 859, 1175, 1424, 1534
HphI	GGTGA	7 cut(s)	367, 896, 1573, 1726, 1745, 1765, 1844
Hpy166II	GTNNAC	4 cut(s)	795, 1385, 1667, 1941
Hpy188I	TCNGA	6 cut(s)	922, 1784, 1792, 1873, 1918, 2041
Hpy188III	TCNNGA	9 cut(s)	341, 413, 478, 524, 957, 965, 1172, 1502, 1994
Hpy8I	GTNNAC	4 cut(s)	795, 1385, 1667, 1941
Hpy99I	CGWCG	3 cut(s)	178, 1389, 2092
HpyAV	CCTTC	5 cut(s)	132, 973, 1448, 1789, 2000
HpyCH4III	ACNGT	4 cut(s)	71, 160, 1462, 1647
HpyCH4IV	ACGT	2 cut(s)	1387, 1836
HpyF10VI	GCNNNNNNNGC	9 cut(s)	35, 115, 272, 281, 518, 806, 887, 1346, 1376
HpyF3I	CTNAG	8 cut(s)	75, 414, 461, 786, 913, 921, 1187, 1710
HpySE526I	ACGT	2 cut(s)	1387, 1836
Hsp92I	GRCGYC	2 cut(s)	173, 1836
Hsp92II	CATG	8 cut(s)	42, 631, 1268, 1318, 1517, 1547, 1602, 1806
KflI	GGGWCCC	1 cut(s)	2071
Ksp22I	TGATCA	1 cut(s)	289
Kzo9I	GATC	9 cut(s)	289, 967, 1251, 1342, 1618, 1629, 1695, 1746, 1977
LguI	GCTCTTC	1 cut(s)	135
LmnI	GCTCC	3 cut(s)	245, 263, 477
Lsp1109I	GCAGC	9 cut(s)	38, 445, 521, 1160, 1379, 1651, 1739, 1753, 2065
LweI	GCATC	4 cut(s)	38, 118, 1537, 1807
MaeI	CTAG	3 cut(s)	179, 866, 1269
MaeII	ACGT	2 cut(s)	1387, 1836
MaeIII	GTNAC	8 cut(s)	3, 148, 355, 709, 1561, 1714, 1771, 1832
MalI	GATC	9 cut(s)	291, 969, 1253, 1344, 1620, 1631, 1697, 1748, 1979
MbiI	CCGCTC	2 cut(s)	266, 1830
MboI	GATC	9 cut(s)	289, 967, 1251, 1342, 1618, 1629, 1695, 1746, 1977
MboII	GAAGA	9 cut(s)	122, 213, 216, 294, 297, 539, 821, 1254, 1898
MfeI	CAATTG	2 cut(s)	876, 1428
MflI	RGATCY	1 cut(s)	1251
MhlI	GDGCHC	5 cut(s)	250, 474, 539, 811, 2032
MlsI	TGGCCA	1 cut(s)	1760
MluNI	TGGCCA	1 cut(s)	1760
MlyI	GAGTC	4 cut(s)	524, 853, 1169, 1528
MmeI	TCCRAC	1 cut(s)	1012
Mox20I	TGGCCA	1 cut(s)	1760
MroXI	GAANNNNTTC	1 cut(s)	1294
MscI	TGGCCA	1 cut(s)	1760
MseI	TTAA	9 cut(s)	20, 549, 584, 776, 975, 1089, 1256, 1359, 1905
MslI	CAYNNNNRTG	2 cut(s)	37, 707
Msp20I	TGGCCA	1 cut(s)	1760
MspA1I	CMGCKG	2 cut(s)	436, 1730
MunI	CAATTG	2 cut(s)	876, 1428
Mva1269I	GAATGC	2 cut(s)	277, 997
MvaI	CCWGG	5 cut(s)	325, 639, 648, 1194, 1539
MwoI	GCNNNNNNNGC	9 cut(s)	35, 115, 272, 281, 518, 806, 887, 1346, 1376
NciI	CCSGG	8 cut(s)	456, 622, 846, 1326, 1961, 2014, 2027, 2069
NcoI	CCATGG	1 cut(s)	1802
NdeI	CATATG	1 cut(s)	1927
NdeII	GATC	9 cut(s)	289, 967, 1251, 1342, 1618, 1629, 1695, 1746, 1977
NheI	GCTAGC	2 cut(s)	865, 1268
NlaIII	CATG	8 cut(s)	42, 631, 1268, 1318, 1517, 1547, 1602, 1806
NlaIV	GGNNCC	6 cut(s)	385, 425, 696, 850, 2072, 2073
NmuCI	GTSAC	8 cut(s)	3, 148, 355, 709, 1561, 1714, 1771, 1832
NspI	RCATGY	2 cut(s)	42, 1517
OliI	CACNNNNGTG	1 cut(s)	707
PaeI	GCATGC	2 cut(s)	42, 1517
PaqCI	CACCTGC	1 cut(s)	1201
PceI	AGGCCT	1 cut(s)	1192
PciSI	GCTCTTC	1 cut(s)	135
PctI	GAATGC	2 cut(s)	277, 997
PdmI	GAANNNNTTC	1 cut(s)	1294
PfeI	GAWTC	1 cut(s)	1424
PfoI	TCCNGGA	1 cut(s)	844
PleI	GAGTC	4 cut(s)	524, 853, 1169, 1528
PpsI	GAGTC	4 cut(s)	524, 853, 1169, 1528
PpuMI	RGGWCCY	2 cut(s)	1987, 2071
PshAI	GACNNNNGTC	2 cut(s)	1568, 1804
Psp124BI	GAGCTC	1 cut(s)	474
Psp5II	RGGWCCY	2 cut(s)	1987, 2071
Psp6I	CCWGG	5 cut(s)	323, 637, 646, 1192, 1537
PspEI	GGTNACC	1 cut(s)	1771
PspFI	CCCAGC	1 cut(s)	728
PspGI	CCWGG	5 cut(s)	323, 637, 646, 1192, 1537
PspN4I	GGNNCC	6 cut(s)	385, 425, 696, 850, 2072, 2073
PspPI	GGNCC	6 cut(s)	635, 644, 839, 1799, 1987, 2071
PspPPI	RGGWCCY	2 cut(s)	1987, 2071
PstI	CTGCAG	2 cut(s)	1615, 2083
PstNI	CAGNNNCTG	2 cut(s)	920, 1645
PsuI	RGATCY	1 cut(s)	1251
PvuII	CAGCTG	1 cut(s)	1730
RsaI	GTAC	1 cut(s)	73
RsaNI	GTAC	1 cut(s)	72
RseI	CAYNNNNRTG	2 cut(s)	37, 707
SacI	GAGCTC	1 cut(s)	474
SalI	GTCGAC	1 cut(s)	1383
SapI	GCTCTTC	1 cut(s)	135
SaqAI	TTAA	9 cut(s)	20, 549, 584, 776, 975, 1089, 1256, 1359, 1905
Sau3AI	GATC	9 cut(s)	289, 967, 1251, 1342, 1618, 1629, 1695, 1746, 1977
Sau96I	GGNCC	6 cut(s)	635, 644, 839, 1799, 1987, 2071
SchI	GAGTC	4 cut(s)	524, 853, 1169, 1528
SduI	GDGCHC	5 cut(s)	250, 474, 539, 811, 2032
SfaNI	GCATC	4 cut(s)	38, 118, 1537, 1807
SfcI	CTRYAG	3 cut(s)	1611, 2079, 2094
SinI	GGWCC	4 cut(s)	635, 1799, 1987, 2071
SmiMI	CAYNNNNRTG	2 cut(s)	37, 707
SmlI	CTYRAG	3 cut(s)	215, 522, 607
SmoI	CTYRAG	3 cut(s)	215, 522, 607
SphI	GCATGC	2 cut(s)	42, 1517
SseBI	AGGCCT	1 cut(s)	1192
SsiI	CCGC	5 cut(s)	266, 282, 436, 1683, 1830
SspMI	CTAG	3 cut(s)	179, 866, 1269
SstI	GAGCTC	1 cut(s)	474
StuI	AGGCCT	1 cut(s)	1192
StyI	CCWWGG	2 cut(s)	984, 1802
TaaI	ACNGT	4 cut(s)	71, 160, 1462, 1647
TaiI	ACGT	2 cut(s)	1390, 1839
TaqI	TCGA	5 cut(s)	501, 768, 811, 1237, 1384
TauI	GCSGC	2 cut(s)	284, 1685
TfiI	GAWTC	1 cut(s)	1424
Tru1I	TTAA	9 cut(s)	20, 549, 584, 776, 975, 1089, 1256, 1359, 1905
Tru9I	TTAA	9 cut(s)	20, 549, 584, 776, 975, 1089, 1256, 1359, 1905
TscAI	CASTG	4 cut(s)	802, 1135, 1339, 1756
TseFI	GTSAC	8 cut(s)	3, 148, 355, 709, 1561, 1714, 1771, 1832
TseI	GCWGC	9 cut(s)	26, 433, 509, 1148, 1367, 1639, 1727, 1741, 2078
Tsp45I	GTSAC	8 cut(s)	3, 148, 355, 709, 1561, 1714, 1771, 1832
TspDTI	ATGAA	9 cut(s)	606, 753, 1032, 1086, 1253, 1377, 1395, 1935, 2034
TspGWI	ACGGA	1 cut(s)	2076
TspRI	CASTG	4 cut(s)	802, 1135, 1339, 1756
VpaK11BI	GGWCC	4 cut(s)	635, 1799, 1987, 2071
XagI	CCTNNNNNAGG	2 cut(s)	417, 1186
XapI	RAATTY	4 cut(s)	82, 813, 2021, 2042
XceI	RCATGY	2 cut(s)	42, 1517
XcmI	CCANNNNNNNNNTGG	1 cut(s)	688
XmiI	GTMKAC	1 cut(s)	1384
XmnI	GAANNNNTTC	1 cut(s)	1294
XspI	CTAG	3 cut(s)	179, 866, 1269
ZraI	GACGTC	1 cut(s)	1837

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.