Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0005588

Physical Location & Seq

Genomic Coordinates Forward (+)

32450180 .. 32453515

Gene Structure 3336 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1263600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2103 bp

ATGAGTGAAGATCTTGATATGGCAGCATCATCAGCATGCCTTGTGGGGAATGGGTTATCTACCCGCGGTACTAAGACAAATTTGAGCAAGGACTTTTGTGGCAGGCATCTCTTGCACCCTTCAGCTCTTCCATCCTTGGGTAAAGTGACAAAAACAGTTCTCACAAAGGCGTCGCTAGACCAAAGGCAAAACGAGGGAAGAAGAGGCTTTCTGAAGTTGTTGCTTGGGAATGTGGGAATAGGAGCACCAGCTTTACTTGGAGCAGGGAATGCTTTGGCCGCCGATGATCAGGGCGTCTCTTCTTCAAGGATGTCTTATTCCAGGTTCTTGGAGTATCTGGACAAAGGCAGGGTGACTAAAGTTGATTTGTTTGAGAATGGAACCATAGCTATTGTTGAGGCTGTTTCTCCTGAGTTAGGGAACCGATTGCAGCGAGTTCGTGTTCAACTTCCGGGACTAAGCCAAGAGCTCCTCCAGAAGTTCAGGGAAAAGAATATCGACTTTGCTGCACATAATGCTCAAGAAGACTCAGGCTCTCTTTTATTTAACCTGATTGGAAATCTTGCTTTCCCACTAATTTTAATTGGAGGCTTGTTCCTACTCTCAAGGCGTTCATCTGGAGGCATGGGTGGTCCAGGTGGGCCTGGATTTCCTCTACAATTTGGTCAGTCTAAAGCCAAGTTCCAAATGGAACCAAACACCGGAGTGACTTTCGATGATGTTGCTGGGGTAGATGAAGCCAAGCAAGATTTCGTGGAGGTGGTCGAATTCTTAAAGAAGCCTGAGAGGTTCACAGCTGTTGGGGCTCGAATTCCTAAAGGGGTTCTTCTTGTTGGCCCTCCAGGAACCGGAAAGACTCTGCTAGCAAAGGCAATTGCTGGTGAAGCGGGTGTTCCATTTTTCTCCATCTCAGGTTCTGAATTTGTTGAAATGTTTGTTGGTGTTGGTGCTTCCCGAGTTCGTGATCTTTTTAAGAAGGCCAAGGAGAATGCACCTTGCATTGTATTTGTTGATGAAATTGACGCTGTTGGACGGCAAAGAGGAACTGGAATTGGAGGAGGGAACGATGAAAGGGAACAAACCCTTAATCAGCTTTTGACAGAAATGGATGGTTTTGAAGGCAACACTGGTATTATCGTGGTTGCAGCGACTAACAGGGCAGACATTCTTGACTCTGCCTTATTGAGGCCAGGCCGGTTTGACAGGCAGGTGTCTGTTGATGTCCCAGACATACGAGGAAGAACAGAGATCTTAAAGGTTCATGCTAGCAATAAGAAGTTTGATGGAACTGTTTCTCTTGATGTTGTAGCCATGAGAACACCTGGCTTCAGTGGGGCAGATCTCGCAAACCTCCTTAATGAAGCAGCTATATTGGCTGGTCGACGTGGGAAGTCAGCAATTTCATCTAAAGAGATTGATGATTCGATTGATAGAATAGTGGCTGGAATGGAAGGAACAGTAATGACAGATGGAAAGAGTAAAAGTCTGGTGGCATATCACGAAGTTGGGCACGCCATATGTGGGACATTGACTCCAGGGCATGATGCTGTCCAAAAGGTGACATTAGTCCCTCGTGGTCAGGCTCGGGGTCTTACGTGGTTTATTCCTGCAGATGATCCTACCTTGATCTCCAAGCAGCAGTTATTTGCAAGAATTGTTGGCGGGCTTGGTGGTAGAGCTGCAGAGGAAGTGATCTTTGGTGAGCCTGAGGTGACAACAGGTGCAGCTGGTGATTTGCAGCAGATCACTGGTTTGGCCAAACAGATGGTTACCACCTTCGGAATGTCTGATATTGGACCGTGGTCATTGATGGACGCGTCTCAGAGCGGTGATGTCATTATGAGAATGATGGCAAGGAACTCAATGTCAGAAAAGCTTGCTGAAGACATTGATTCAGCTATTAAGAGATTATCGGATGAAGCATATGAGATTGCGTTGACTCACATAAGAAACAACCGCGAAGCCATTGACAAGATCGTGGAGGTCCTTCTTGAGAAGGAAACAATGACCGGAGATGAATTCCGGGCAATCTTGTCCGAATTCGTTGAAATCCCAGTTGAAAACCGGGTCCCTGCTGCAGTTCCAACACCAGTGGCTGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004176 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006091 GO:0006508 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009056 GO:0009057 GO:0009314 GO:0009416 GO:0009507 GO:0009526 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009628 GO:0009642 GO:0009644 GO:0009657 GO:0009658 GO:0009668 GO:0009719 GO:0009725 GO:0009735 GO:0009765 GO:0009892 GO:0009941 GO:0009987 GO:0010027 GO:0010033 GO:0010109 GO:0010205 GO:0010206 GO:0010304 GO:0015979 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0019538 GO:0019684 GO:0022607 GO:0030091 GO:0030163 GO:0031323 GO:0031324 GO:0031967 GO:0031975 GO:0031976 GO:0031977 GO:0031984 GO:0034357 GO:0034622 GO:0042221 GO:0042548 GO:0042623 GO:0042651 GO:0043155 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043467 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0048519 GO:0048523 GO:0048564 GO:0050789 GO:0050794 GO:0050896 GO:0055035 GO:0061024 GO:0065003 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072593 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1905156

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA FtsH_ext Peptidase_M41

Protein Analysis

701

Amino Acids

74.67

Weight (kDa)

5.58

Isoelectric Point (pI)

32.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FtsH_ext	PF06480	95 - 170	2.3e-07	FtsH Extracellular
AAA	PF00004	275 - 407	4.1e-46	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	431 - 473	3.3e-12	AAA+ lid domain
Peptidase_M41	PF01434	489 - 677	1.4e-67	Peptidase family M41

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005588)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06430 AT1G06430 AT1G06430 AT2G30950 AT2G30950 AT2G30950 AT2G30950
fragaria_vesca	FvH4_6g27780 FvH4_6g27780 FvH4_6g27780
malus_domestica	MD09G1234500.v1.1 MD17G1263600.v1.1
prunus_persica	Prupe.3G112700_v2.0.a1 Prupe.3G112700_v2.0.a1
pyrus_communis	pycom09g15280 pycom17g26380
rosa_chinensis	RchiOBHm_Chr2g0131061
rosa_roxburghii	Rroxscaffold_2G00113260
rosa_samantha	Rh2AG347000 Rh2AG479700 Rh2BG354500 Rh2BG461700 Rh2CG333000 Rh2DG372500 Rh2DG470900
rosa_wichuraiana	Rw2G028140 Rw2G028180 Rw4G004930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1198
Acc36I	ACCTGC	1 cut(s)	1198
AccBSI	CCGCTC	1 cut(s)	1827
AccI	GTMKAC	1 cut(s)	1381
AccII	CGCG	3 cut(s)	66, 1817, 1959
AciI	CCGC	7 cut(s)	64, 66, 279, 887, 1662, 1827, 1957
AclWI	GGATC	1 cut(s)	1610
AcoI	YGGCCR	2 cut(s)	276, 1755
AcsI	RAATTY	6 cut(s)	79, 767, 810, 920, 2018, 2039
AcuI	CTGAAG	4 cut(s)	105, 233, 1312, 1902
AcyI	GRCGYC	2 cut(s)	170, 294
AfaI	GTAC	1 cut(s)	70
AfiI	CCNNNNNNNGG	6 cut(s)	137, 416, 701, 848, 1185, 1521
AflIII	ACRYGT	1 cut(s)	1815
AgsI	TTSAA	6 cut(s)	306, 446, 929, 1118, 2048, 2060
AjiI	CACGTC	1 cut(s)	1385
AjnI	CCWGG	7 cut(s)	320, 634, 643, 841, 1189, 1321, 1534
AjuI	GAANNNNNNNTTGG	6 cut(s)	734, 766, 921, 953, 1680, 1712
AleI	CACNNNNGTG	1 cut(s)	704
Alw21I	GWGCWC	2 cut(s)	247, 471
Alw26I	GTCTC	2 cut(s)	301, 1824
AlwI	GGATC	1 cut(s)	1610
AlwNI	CAGNNNCTG	2 cut(s)	917, 2096
Ama87I	CYCGRG	2 cut(s)	954, 1584
AoxI	GGCC	7 cut(s)	276, 641, 835, 978, 1187, 1192, 1755
ApoI	RAATTY	6 cut(s)	79, 767, 810, 920, 2018, 2039
Asp700I	GAANNNNTTC	1 cut(s)	1291
AspS9I	GGNCC	6 cut(s)	632, 641, 836, 1796, 1984, 2068
AsuC2I	CCSGG	3 cut(s)	453, 2024, 2066
AsuHPI	GGTGA	7 cut(s)	364, 893, 1570, 1712, 1723, 1742, 1841
AsuNHI	GCTAGC	2 cut(s)	862, 1265
AvaI	CYCGRG	2 cut(s)	954, 1584
AvaII	GGWCC	4 cut(s)	632, 1796, 1984, 2068
AxyI	CCTNAGG	1 cut(s)	1707
BaeGI	GKGCMC	1 cut(s)	1512
BalI	TGGCCA	1 cut(s)	1757
BanII	GRGCYC	2 cut(s)	471, 808
BauI	CACGAG	1 cut(s)	1572
BbsI	GAAGAC	2 cut(s)	531, 1890
Bbv12I	GWGCWC	2 cut(s)	247, 471
BccI	CCATC	8 cut(s)	139, 914, 1103, 1277, 1463, 1759, 1804, 1843
BceAI	ACGGC	1 cut(s)	1049
BcgI	CGANNNNNNTGC	6 cut(s)	419, 453, 488, 522, 704, 738
BciT130I	CCWGG	7 cut(s)	322, 636, 645, 843, 1191, 1323, 1536
BclI	TGATCA	1 cut(s)	286
BcnI	CCSGG	3 cut(s)	453, 2024, 2066
BcoDI	GTCTC	2 cut(s)	301, 1824
BfaI	CTAG	3 cut(s)	176, 863, 1266
BfmI	CTRYAG	3 cut(s)	1608, 1680, 2076
BfuAI	ACCTGC	1 cut(s)	1198
BglII	AGATCT	3 cut(s)	10, 1248, 1339
Bme18I	GGWCC	4 cut(s)	632, 1796, 1984, 2068
BmeT110I	CYCGRG	2 cut(s)	954, 1584
BmgBI	CACGTC	1 cut(s)	1385
BmgT120I	GGNCC	6 cut(s)	632, 641, 836, 1796, 1984, 2068
BmiI	GGNNCC	6 cut(s)	382, 422, 693, 847, 2069, 2070
BmrI	ACTGGG	1 cut(s)	2048
BmsI	GCATC	3 cut(s)	35, 115, 1534
BmtI	GCTAGC	2 cut(s)	866, 1269
BmuI	ACTGGG	1 cut(s)	2048
BoxI	GACNNNNGTC	2 cut(s)	1565, 1801
BpiI	GAAGAC	2 cut(s)	531, 1890
BpmI	CTGGAG	4 cut(s)	458, 639, 825, 1518
BpuEI	CTTGAG	3 cut(s)	504, 589, 2012
BpuMI	CCSGG	3 cut(s)	453, 2024, 2066
BsaAI	YACGTR	1 cut(s)	1596
BsaHI	GRCGYC	2 cut(s)	170, 294
BsaJI	CCNNGG	5 cut(s)	64, 135, 981, 1535, 1799
BsaWI	WCCGGW	3 cut(s)	701, 848, 2009
BsaXI	ACNNNNNCTCC	2 cut(s)	1516, 1546
Bsc4I	CCNNNNNNNGG	6 cut(s)	137, 416, 701, 848, 1185, 1521
Bse118I	RCCGGY	1 cut(s)	1194
Bse1I	ACTGG	5 cut(s)	1051, 1132, 1753, 2054, 2090
Bse21I	CCTNAGG	1 cut(s)	1707
BseBI	CCWGG	7 cut(s)	322, 636, 645, 843, 1191, 1323, 1536
BseDI	CCNNGG	5 cut(s)	64, 135, 981, 1535, 1799
BseGI	GGATG	4 cut(s)	131, 315, 1114, 1921
BseLI	CCNNNNNNNGG	6 cut(s)	137, 416, 701, 848, 1185, 1521
BseMII	CTCAG	6 cut(s)	402, 543, 774, 924, 1698, 1835
BseNI	ACTGG	5 cut(s)	1051, 1132, 1753, 2054, 2090
BseRI	GAGGAG	2 cut(s)	461, 1071
BseSI	GKGCMC	1 cut(s)	1512
BseYI	CCCAGC	1 cut(s)	725
BsgI	GTGCAG	2 cut(s)	492, 1743
Bsh1236I	CGCG	3 cut(s)	66, 1817, 1959
BshFI	GGCC	7 cut(s)	278, 643, 837, 980, 1189, 1194, 1757
BsiHKAI	GWGCWC	2 cut(s)	247, 471
BsiHKCI	CYCGRG	2 cut(s)	954, 1584
BsiSI	CCGG	7 cut(s)	452, 702, 849, 1195, 2010, 2023, 2065
BslFI	GGGAC	5 cut(s)	468, 1208, 1537, 1553, 2054
BslI	CCNNNNNNNGG	6 cut(s)	137, 416, 701, 848, 1185, 1521
BsmAI	GTCTC	2 cut(s)	301, 1824
BsmBI	CGTCTC	2 cut(s)	301, 1824
BsmFI	GGGAC	5 cut(s)	468, 1208, 1537, 1553, 2054
BsmI	GAATGC	2 cut(s)	274, 994
BsnI	GGCC	7 cut(s)	278, 643, 837, 980, 1189, 1194, 1757
BsoBI	CYCGRG	2 cut(s)	954, 1584
Bsp1286I	GDGCHC	4 cut(s)	247, 471, 808, 1512
BspACI	CCGC	7 cut(s)	64, 66, 279, 887, 1662, 1827, 1957
BspANI	GGCC	7 cut(s)	278, 643, 837, 980, 1189, 1194, 1757
BspCNI	CTCAG	6 cut(s)	403, 542, 775, 923, 1699, 1834
BspFNI	CGCG	3 cut(s)	66, 1817, 1959
BspLI	GGNNCC	6 cut(s)	382, 422, 693, 847, 2069, 2070
BspMAI	CTGCAG	3 cut(s)	1612, 1684, 2080
BspMI	ACCTGC	1 cut(s)	1198
BspOI	GCTAGC	2 cut(s)	866, 1269
BspPI	GGATC	1 cut(s)	1610
BspQI	GCTCTTC	1 cut(s)	132
BsrBI	CCGCTC	1 cut(s)	1827
BsrFI	RCCGGY	1 cut(s)	1194
BsrI	ACTGG	5 cut(s)	1051, 1132, 1753, 2054, 2090
BssAI	RCCGGY	1 cut(s)	1194
BssECI	CCNNGG	5 cut(s)	64, 135, 981, 1535, 1799
BssNI	GRCGYC	2 cut(s)	170, 294
BssSI	CACGAG	1 cut(s)	1572
BssT1I	CCWWGG	2 cut(s)	135, 981
Bst2BI	CACGAG	1 cut(s)	1572
Bst2UI	CCWGG	7 cut(s)	322, 636, 645, 843, 1191, 1323, 1536
Bst4CI	ACNGT	4 cut(s)	157, 1291, 1459, 1800
Bst6I	CTCTTC	3 cut(s)	132, 196, 304
BstACI	GRCGYC	2 cut(s)	170, 294
BstAPI	GCANNNNNTGC	3 cut(s)	112, 269, 515
BstBAI	YACGTR	1 cut(s)	1596
BstC8I	GCNNGC	7 cut(s)	37, 104, 864, 1267, 1512, 1664, 1878
BstDEI	CTNAG	8 cut(s)	72, 411, 458, 529, 783, 910, 1707, 1821
BstDSI	CCRYGG	2 cut(s)	64, 1799
BstEII	GGTNACC	1 cut(s)	1768
BstENI	CCTNNNNNAGG	2 cut(s)	414, 1183
BstF5I	GGATG	4 cut(s)	131, 315, 1114, 1921
BstFNI	CGCG	3 cut(s)	66, 1817, 1959
BstMAI	GTCTC	2 cut(s)	301, 1824
BstMWI	GCNNNNNNNGC	9 cut(s)	32, 112, 269, 278, 515, 803, 884, 1343, 1373
BstNI	CCWGG	7 cut(s)	322, 636, 645, 843, 1191, 1323, 1536
BstNSI	RCATGY	1 cut(s)	39
BstPAI	GACNNNNGTC	2 cut(s)	1565, 1801
BstPI	GGTNACC	1 cut(s)	1768
BstSFI	CTRYAG	3 cut(s)	1608, 1680, 2076
BstSLI	GKGCMC	1 cut(s)	1512
BstUI	CGCG	3 cut(s)	66, 1817, 1959
BstV2I	GAAGAC	2 cut(s)	531, 1890
BstX2I	RGATCY	3 cut(s)	10, 1248, 1339
BstXI	CCANNNNNNTGG	2 cut(s)	328, 1765
BstYI	RGATCY	3 cut(s)	10, 1248, 1339
Bsu36I	CCTNAGG	1 cut(s)	1707
BsuRI	GGCC	7 cut(s)	278, 643, 837, 980, 1189, 1194, 1757
BtgI	CCRYGG	2 cut(s)	64, 1799
BtrI	CACGTC	1 cut(s)	1385
BtsCI	GGATG	4 cut(s)	131, 315, 1114, 1921
BtsIMutI	CAGTG	4 cut(s)	1125, 1336, 1746, 2097
BveI	ACCTGC	1 cut(s)	1198
Cac8I	GCNNGC	7 cut(s)	37, 104, 864, 1267, 1512, 1664, 1878
CaiI	CAGNNNCTG	2 cut(s)	917, 2096
Cfr10I	RCCGGY	1 cut(s)	1194
Cfr13I	GGNCC	6 cut(s)	632, 641, 836, 1796, 1984, 2068
Cfr42I	CCGCGG	1 cut(s)	67
CseI	GACGC	5 cut(s)	159, 283, 1031, 1806, 1823
Csp6I	GTAC	1 cut(s)	69
CspCI	CAANNNNNGTGG	1 cut(s)	2073
CviAII	CATG	5 cut(s)	36, 625, 1262, 1312, 1541
CviQI	GTAC	1 cut(s)	69
DdeI	CTNAG	8 cut(s)	72, 411, 458, 529, 783, 910, 1707, 1821
EaeI	YGGCCR	2 cut(s)	276, 1755
Eam1104I	CTCTTC	3 cut(s)	132, 196, 304
EarI	CTCTTC	3 cut(s)	132, 196, 304
Ecl136II	GAGCTC	1 cut(s)	469
Eco130I	CCWWGG	2 cut(s)	135, 981
Eco24I	GRGCYC	2 cut(s)	471, 808
Eco47I	GGWCC	4 cut(s)	632, 1796, 1984, 2068
Eco53kI	GAGCTC	1 cut(s)	469
Eco57I	CTGAAG	4 cut(s)	105, 233, 1312, 1902
Eco81I	CCTNAGG	1 cut(s)	1707
Eco88I	CYCGRG	2 cut(s)	954, 1584
Eco91I	GGTNACC	1 cut(s)	1768
EcoICRI	GAGCTC	1 cut(s)	469
EcoNI	CCTNNNNNAGG	2 cut(s)	414, 1183
EcoO109I	RGGNCCY	2 cut(s)	1984, 2068
EcoO65I	GGTNACC	1 cut(s)	1768
EcoRI	GAATTC	4 cut(s)	767, 810, 2018, 2039
EcoRII	CCWGG	7 cut(s)	320, 634, 643, 841, 1189, 1321, 1534
EcoT14I	CCWWGG	2 cut(s)	135, 981
EcoT38I	GRGCYC	2 cut(s)	471, 808
ErhI	CCWWGG	2 cut(s)	135, 981
Esp3I	CGTCTC	2 cut(s)	301, 1824
FaeI	CATG	5 cut(s)	39, 628, 1265, 1315, 1544
FalI	AAGNNNNNCTT	4 cut(s)	298, 330, 810, 842
FaqI	GGGAC	5 cut(s)	468, 1208, 1537, 1553, 2054
FatI	CATG	5 cut(s)	35, 624, 1261, 1311, 1540
FauI	CCCGC	3 cut(s)	71, 880, 1655
FauNDI	CATATG	2 cut(s)	1517, 1924
FbaI	TGATCA	1 cut(s)	286
FblI	GTMKAC	1 cut(s)	1381
FokI	GGATG	4 cut(s)	118, 322, 1121, 1928
FriOI	GRGCYC	2 cut(s)	471, 808
FspBI	CTAG	3 cut(s)	176, 863, 1266
GsaI	CCCAGC	1 cut(s)	729
GsuI	CTGGAG	4 cut(s)	458, 639, 825, 1518
HaeIII	GGCC	7 cut(s)	278, 643, 837, 980, 1189, 1194, 1757
HapII	CCGG	7 cut(s)	452, 702, 849, 1195, 2010, 2023, 2065
HgaI	GACGC	5 cut(s)	159, 283, 1031, 1806, 1823
Hin1I	GRCGYC	2 cut(s)	170, 294
Hin1II	CATG	5 cut(s)	39, 628, 1265, 1315, 1544
HincII	GTYRAC	2 cut(s)	1382, 1938
HindII	GTYRAC	2 cut(s)	1382, 1938
HindIII	AAGCTT	1 cut(s)	1874
HinfI	GANTC	7 cut(s)	527, 856, 1172, 1421, 1531, 1892, 1939
HpaII	CCGG	7 cut(s)	452, 702, 849, 1195, 2010, 2023, 2065
HphI	GGTGA	7 cut(s)	364, 893, 1570, 1712, 1723, 1742, 1841
Hpy166II	GTNNAC	3 cut(s)	792, 1382, 1938
Hpy188I	TCNGA	8 cut(s)	213, 919, 1781, 1789, 1824, 1870, 1915, 2038
Hpy8I	GTNNAC	3 cut(s)	792, 1382, 1938
Hpy99I	CGWCG	2 cut(s)	175, 1386
HpyAV	CCTTC	7 cut(s)	129, 970, 1112, 1445, 1786, 1990, 1997
HpyCH4III	ACNGT	4 cut(s)	157, 1291, 1459, 1800
HpyCH4IV	ACGT	2 cut(s)	1384, 1595
HpyF10VI	GCNNNNNNNGC	9 cut(s)	32, 112, 269, 278, 515, 803, 884, 1343, 1373
HpyF3I	CTNAG	8 cut(s)	72, 411, 458, 529, 783, 910, 1707, 1821
HpySE526I	ACGT	2 cut(s)	1384, 1595
Hsp92I	GRCGYC	2 cut(s)	170, 294
Hsp92II	CATG	5 cut(s)	39, 628, 1265, 1315, 1544
KflI	GGGWCCC	1 cut(s)	2068
Ksp22I	TGATCA	1 cut(s)	286
KspI	CCGCGG	1 cut(s)	67
LguI	GCTCTTC	1 cut(s)	132
LmnI	GCTCC	3 cut(s)	242, 260, 474
LweI	GCATC	3 cut(s)	35, 115, 1534
MaeI	CTAG	3 cut(s)	176, 863, 1266
MaeII	ACGT	2 cut(s)	1384, 1595
MaeIII	GTNAC	6 cut(s)	145, 352, 706, 1558, 1711, 1768
MbiI	CCGCTC	1 cut(s)	1827
MfeI	CAATTG	1 cut(s)	873
MflI	RGATCY	3 cut(s)	10, 1248, 1339
MhlI	GDGCHC	4 cut(s)	247, 471, 808, 1512
MlsI	TGGCCA	1 cut(s)	1757
MluI	ACGCGT	1 cut(s)	1815
MluNI	TGGCCA	1 cut(s)	1757
MlyI	GAGTC	5 cut(s)	521, 850, 1166, 1525, 1933
MmeI	TCCRAC	1 cut(s)	1009
Mox20I	TGGCCA	1 cut(s)	1757
MroXI	GAANNNNTTC	1 cut(s)	1291
MscI	TGGCCA	1 cut(s)	1757
MseI	TTAA	8 cut(s)	546, 581, 773, 972, 1086, 1253, 1356, 1902
MslI	CAYNNNNRTG	2 cut(s)	34, 704
Msp20I	TGGCCA	1 cut(s)	1757
MspA1I	CMGCKG	3 cut(s)	66, 797, 1727
MspI	CCGG	7 cut(s)	452, 702, 849, 1195, 2010, 2023, 2065
MunI	CAATTG	1 cut(s)	873
Mva1269I	GAATGC	2 cut(s)	274, 994
MvaI	CCWGG	7 cut(s)	322, 636, 645, 843, 1191, 1323, 1536
MvnI	CGCG	3 cut(s)	66, 1817, 1959
MwoI	GCNNNNNNNGC	9 cut(s)	32, 112, 269, 278, 515, 803, 884, 1343, 1373
NciI	CCSGG	3 cut(s)	453, 2024, 2066
NdeI	CATATG	2 cut(s)	1517, 1924
NheI	GCTAGC	2 cut(s)	862, 1265
NlaIII	CATG	5 cut(s)	39, 628, 1265, 1315, 1544
NlaIV	GGNNCC	6 cut(s)	382, 422, 693, 847, 2069, 2070
NmuCI	GTSAC	5 cut(s)	145, 352, 706, 1558, 1711
NspI	RCATGY	1 cut(s)	39
OliI	CACNNNNGTG	1 cut(s)	704
PaeI	GCATGC	1 cut(s)	39
PaqCI	CACCTGC	1 cut(s)	1198
PciSI	GCTCTTC	1 cut(s)	132
PctI	GAATGC	2 cut(s)	274, 994
PdmI	GAANNNNTTC	1 cut(s)	1291
PfeI	GAWTC	2 cut(s)	1421, 1892
PfoI	TCCNGGA	2 cut(s)	451, 841
PleI	GAGTC	5 cut(s)	521, 850, 1166, 1525, 1933
PpsI	GAGTC	5 cut(s)	521, 850, 1166, 1525, 1933
Ppu21I	YACGTR	1 cut(s)	1596
PpuMI	RGGWCCY	2 cut(s)	1984, 2068
PshAI	GACNNNNGTC	2 cut(s)	1565, 1801
Psp124BI	GAGCTC	1 cut(s)	471
Psp5II	RGGWCCY	2 cut(s)	1984, 2068
Psp6I	CCWGG	7 cut(s)	320, 634, 643, 841, 1189, 1321, 1534
PspEI	GGTNACC	1 cut(s)	1768
PspFI	CCCAGC	1 cut(s)	725
PspGI	CCWGG	7 cut(s)	320, 634, 643, 841, 1189, 1321, 1534
PspN4I	GGNNCC	6 cut(s)	382, 422, 693, 847, 2069, 2070
PspPI	GGNCC	6 cut(s)	632, 641, 836, 1796, 1984, 2068
PspPPI	RGGWCCY	2 cut(s)	1984, 2068
PstI	CTGCAG	3 cut(s)	1612, 1684, 2080
PstNI	CAGNNNCTG	2 cut(s)	917, 2096
PsuI	RGATCY	3 cut(s)	10, 1248, 1339
PvuII	CAGCTG	2 cut(s)	797, 1727
RsaI	GTAC	1 cut(s)	70
RsaNI	GTAC	1 cut(s)	69
RseI	CAYNNNNRTG	2 cut(s)	34, 704
SacI	GAGCTC	1 cut(s)	471
SacII	CCGCGG	1 cut(s)	67
SalI	GTCGAC	1 cut(s)	1380
SapI	GCTCTTC	1 cut(s)	132
SaqAI	TTAA	8 cut(s)	546, 581, 773, 972, 1086, 1253, 1356, 1902
Sau96I	GGNCC	6 cut(s)	632, 641, 836, 1796, 1984, 2068
SchI	GAGTC	5 cut(s)	521, 850, 1166, 1525, 1933
SduI	GDGCHC	4 cut(s)	247, 471, 808, 1512
SfaNI	GCATC	3 cut(s)	35, 115, 1534
SfcI	CTRYAG	3 cut(s)	1608, 1680, 2076
Sfr303I	CCGCGG	1 cut(s)	67
SgrBI	CCGCGG	1 cut(s)	67
SinI	GGWCC	4 cut(s)	632, 1796, 1984, 2068
SmiMI	CAYNNNNRTG	2 cut(s)	34, 704
SmlI	CTYRAG	3 cut(s)	519, 604, 1991
SmoI	CTYRAG	3 cut(s)	519, 604, 1991
SphI	GCATGC	1 cut(s)	39
SsiI	CCGC	7 cut(s)	64, 66, 279, 887, 1662, 1827, 1957
SspMI	CTAG	3 cut(s)	176, 863, 1266
SstI	GAGCTC	1 cut(s)	471
StyI	CCWWGG	2 cut(s)	135, 981
TaaI	ACNGT	4 cut(s)	157, 1291, 1459, 1800
TaiI	ACGT	2 cut(s)	1387, 1598
TaqI	TCGA	6 cut(s)	498, 714, 765, 808, 1381, 1424
TauI	GCSGC	1 cut(s)	281
TfiI	GAWTC	2 cut(s)	1421, 1892
Tru1I	TTAA	8 cut(s)	546, 581, 773, 972, 1086, 1253, 1356, 1902
Tru9I	TTAA	8 cut(s)	546, 581, 773, 972, 1086, 1253, 1356, 1902
TscAI	CASTG	4 cut(s)	1132, 1336, 1753, 2097
TseFI	GTSAC	5 cut(s)	145, 352, 706, 1558, 1711
Tsp45I	GTSAC	5 cut(s)	145, 352, 706, 1558, 1711
TspDTI	ATGAA	9 cut(s)	603, 750, 1029, 1083, 1250, 1374, 1392, 1932, 2031
TspRI	CASTG	4 cut(s)	1132, 1336, 1753, 2097
VpaK11BI	GGWCC	4 cut(s)	632, 1796, 1984, 2068
XagI	CCTNNNNNAGG	2 cut(s)	414, 1183
XapI	RAATTY	6 cut(s)	79, 767, 810, 920, 2018, 2039
XceI	RCATGY	1 cut(s)	39
XcmI	CCANNNNNNNNNTGG	1 cut(s)	685
XmiI	GTMKAC	1 cut(s)	1381
XmnI	GAANNNNTTC	1 cut(s)	1291
XspI	CTAG	3 cut(s)	176, 863, 1266

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.