MD09G1238100.v1.1

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0014335
Physical Location & Seq
Forward (+)
30191552 .. 30193069
1518 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1238100.v1.1.491

Sequence Viewer

Length: 441 bp
ATGACGACGACGATAGAGATTAGTGTTCACATCGATTGCCCGGGGTGTGAGAGCAAGGTTAGAAATGCACTGCAAAAGCTAAAAGGTGTGGACAACGTTGACATAGACATGGCCATGCAAAAGGTGACGATCACTGGATGGGCTGACCAGAAGAAGGTTCTGAGGATAGTCCGGAAGACTGGTCGGAGGGCTGAGCTGTGGCAGCAACCCCCTTTTGATTCAGAATACAACTTCGATACAGACCAATATTACAGTCAACAACAATGTAATGGCTCTGCACCTCAGCCTTCCTCATCTTACAACTACTACAAGCATGGTTACGACGGTCCTTCGAATTATCATGCTTATTATTCTCAGAATTCTGCAAATTCCAGTTCTTATGGATATCAAACCACTGCGGCCTTTAGCGATGAGAACCCTCATGCTTGTTCTATGATGTGA

Protein Analysis

147

Amino Acids

16.55

Weight (kDa)

6.04

Isoelectric Point (pI)

49.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 9 - 62 6.1e-15 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014335)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06330
fragaria_vesca FvH4_6g27360 FvH4_6g27360
malus_domestica MD09G1238100.v1.1
prunus_persica Prupe.3G118600_v2.0.a1
pyrus_communis pycom09g15660
rosa_chinensis RchiOBHm_Chr2g0129621
rosa_laevigata RLG00000019090
rosa_multiflora Rmu_sc0011456.1_g000002
rosa_roxburghii Rroxscaffold_2G00114510
rosa_rugosa Rorug02G0287500
rosa_samantha Rh2AG339200 Rh2BG348000 Rh2CG326700 Rh2DG366200
rosa_wichuraiana Rw2G027870 Rw2G028010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 171
AciI CCGC 1 cut(s) 398
AclI AACGTT 1 cut(s) 96
AcoI YGGCCR 1 cut(s) 111
AcsI RAATTY 2 cut(s) 358, 367
AfiI CCNNNNNNNGG 1 cut(s) 154
AluBI AGCT 2 cut(s) 79, 196
AluI AGCT 2 cut(s) 79, 196
Ama87I CYCGRG 1 cut(s) 40
Aor13HI TCCGGA 1 cut(s) 171
AoxI GGCC 2 cut(s) 111, 399
ApeKI GCWGC 1 cut(s) 202
ApoI RAATTY 2 cut(s) 358, 367
AspS9I GGNCC 1 cut(s) 326
AsuC2I CCSGG 2 cut(s) 41, 42
AsuHPI GGTGA 1 cut(s) 136
AsuII TTCGAA 1 cut(s) 332
AvaI CYCGRG 1 cut(s) 40
AvaII GGWCC 1 cut(s) 326
BalI TGGCCA 1 cut(s) 113
BbsI GAAGAC 1 cut(s) 182
BbvCI CCTCAGC 1 cut(s) 282
BbvI GCAGC 1 cut(s) 214
BccI CCATC 1 cut(s) 132
BcnI CCSGG 2 cut(s) 41, 42
BisI GCNGC 2 cut(s) 203, 399
BlpI GCTNAGC 1 cut(s) 192
BlsI GCNGC 2 cut(s) 204, 400
Bme1390I CCNGG 2 cut(s) 41, 42
Bme18I GGWCC 1 cut(s) 326
BmeT110I CYCGRG 1 cut(s) 40
BmgT120I GGNCC 1 cut(s) 326
BmrFI CCNGG 2 cut(s) 41, 42
BpiI GAAGAC 1 cut(s) 182
Bpu10I CCTNAGC 1 cut(s) 282
Bpu1102I GCTNAGC 1 cut(s) 192
Bpu14I TTCGAA 1 cut(s) 332
BpuMI CCSGG 2 cut(s) 41, 42
Bsa29I ATCGAT 1 cut(s) 33
BsaJI CCNNGG 2 cut(s) 40, 41
BsaWI WCCGGW 1 cut(s) 171
Bsc4I CCNNNNNNNGG 1 cut(s) 154
Bse1I ACTGG 3 cut(s) 139, 184, 372
BseAI TCCGGA 1 cut(s) 171
BseCI ATCGAT 1 cut(s) 33
BseDI CCNNGG 2 cut(s) 40, 41
BseGI GGATG 1 cut(s) 143
BseLI CCNNNNNNNGG 1 cut(s) 154
BseMII CTCAG 4 cut(s) 152, 183, 296, 368
BseNI ACTGG 3 cut(s) 139, 184, 372
BseXI GCAGC 1 cut(s) 214
BsgI GTGCAG 1 cut(s) 261
BshFI GGCC 2 cut(s) 113, 401
BshVI ATCGAT 1 cut(s) 33
BsiHKCI CYCGRG 1 cut(s) 40
BsiSI CCGG 2 cut(s) 41, 172
BslI CCNNNNNNNGG 1 cut(s) 154
BsnI GGCC 2 cut(s) 113, 401
BsoBI CYCGRG 1 cut(s) 40
Bsp119I TTCGAA 1 cut(s) 332
Bsp13I TCCGGA 1 cut(s) 171
Bsp143I GATC 1 cut(s) 129
Bsp1720I GCTNAGC 1 cut(s) 192
BspACI CCGC 1 cut(s) 398
BspANI GGCC 2 cut(s) 113, 401
BspCNI CTCAG 4 cut(s) 153, 184, 295, 367
BspDI ATCGAT 1 cut(s) 33
BspEI TCCGGA 1 cut(s) 171
BspT104I TTCGAA 1 cut(s) 332
BsrI ACTGG 3 cut(s) 139, 184, 372
BssECI CCNNGG 2 cut(s) 40, 41
BssMI GATC 1 cut(s) 129
Bst4CI ACNGT 2 cut(s) 254, 326
BstBI TTCGAA 1 cut(s) 332
BstDEI CTNAG 4 cut(s) 161, 192, 282, 354
BstF5I GGATG 1 cut(s) 143
BstKTI GATC 1 cut(s) 132
BstMBI GATC 1 cut(s) 129
BstMWI GCNNNNNNNGC 1 cut(s) 202
BstSCI CCNGG 2 cut(s) 39, 40
BstV1I GCAGC 1 cut(s) 214
BstV2I GAAGAC 1 cut(s) 182
Bsu15I ATCGAT 1 cut(s) 33
BsuRI GGCC 2 cut(s) 113, 401
BsuTUI ATCGAT 1 cut(s) 33
BtgZI GCGATG 1 cut(s) 423
BtsCI GGATG 1 cut(s) 143
BtsI GCAGTG 2 cut(s) 68, 393
BtsIMutI CAGTG 3 cut(s) 68, 132, 393
Cfr13I GGNCC 1 cut(s) 326
Cfr9I CCCGGG 1 cut(s) 40
ClaI ATCGAT 1 cut(s) 33
CviAII CATG 5 cut(s) 109, 115, 314, 341, 422
CviJI RGCY 8 cut(s) 79, 113, 143, 191, 196, 273, 286, 401
CviKI_1 RGCY 8 cut(s) 79, 113, 143, 191, 196, 273, 286, 401
DdeI CTNAG 4 cut(s) 161, 192, 282, 354
DpnI GATC 1 cut(s) 131
DpnII GATC 1 cut(s) 129
EaeI YGGCCR 1 cut(s) 111
Eco32I GATATC 1 cut(s) 386
Eco47I GGWCC 1 cut(s) 326
Eco88I CYCGRG 1 cut(s) 40
EcoRI GAATTC 1 cut(s) 358
EcoRV GATATC 1 cut(s) 386
FaeI CATG 5 cut(s) 112, 118, 317, 344, 425
FaiI YATR 8 cut(s) 104, 110, 116, 315, 342, 381, 423, 434
FatI CATG 5 cut(s) 108, 114, 313, 340, 421
Fnu4HI GCNGC 2 cut(s) 203, 399
FokI GGATG 1 cut(s) 150
Fsp4HI GCNGC 2 cut(s) 203, 399
GluI GCNGC 2 cut(s) 203, 399
HaeIII GGCC 2 cut(s) 113, 401
HapII CCGG 2 cut(s) 41, 172
Hin1II CATG 5 cut(s) 112, 118, 317, 344, 425
HincII GTYRAC 2 cut(s) 100, 257
HindII GTYRAC 2 cut(s) 100, 257
HinfI GANTC 1 cut(s) 218
HpaII CCGG 2 cut(s) 41, 172
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 4 cut(s) 28, 91, 100, 257
Hpy188I TCNGA 4 cut(s) 162, 186, 223, 357
Hpy188III TCNNGA 1 cut(s) 172
Hpy8I GTNNAC 4 cut(s) 28, 91, 100, 257
Hpy99I CGWCG 3 cut(s) 10, 13, 326
HpyAV CCTTC 3 cut(s) 148, 297, 339
HpyCH4III ACNGT 2 cut(s) 254, 326
HpyCH4IV ACGT 1 cut(s) 96
HpyCH4V TGCA 5 cut(s) 68, 73, 118, 278, 365
HpyF10VI GCNNNNNNNGC 1 cut(s) 202
HpyF3I CTNAG 4 cut(s) 161, 192, 282, 354
HpySE526I ACGT 1 cut(s) 96
Hsp92II CATG 5 cut(s) 112, 118, 317, 344, 425
Kpn2I TCCGGA 1 cut(s) 171
Kzo9I GATC 1 cut(s) 129
LpnPI CCDG 6 cut(s) 54, 120, 161, 165, 185, 385
Lsp1109I GCAGC 1 cut(s) 214
MaeII ACGT 1 cut(s) 96
MaeIII GTNAC 2 cut(s) 124, 317
MalI GATC 1 cut(s) 131
MboI GATC 1 cut(s) 129
MboII GAAGA 2 cut(s) 163, 187
MlsI TGGCCA 1 cut(s) 113
MluCI AATT 3 cut(s) 334, 358, 367
MluNI TGGCCA 1 cut(s) 113
MmeI TCCRAC 1 cut(s) 164
MnlI CCTC 5 cut(s) 156, 180, 291, 301, 429
Mox20I TGGCCA 1 cut(s) 113
MroI TCCGGA 1 cut(s) 171
MscI TGGCCA 1 cut(s) 113
MslI CAYNNNNRTG 2 cut(s) 107, 113
Msp20I TGGCCA 1 cut(s) 113
MspI CCGG 2 cut(s) 41, 172
MspR9I CCNGG 2 cut(s) 41, 42
MwoI GCNNNNNNNGC 1 cut(s) 202
NciI CCSGG 2 cut(s) 41, 42
NdeII GATC 1 cut(s) 129
NlaIII CATG 5 cut(s) 112, 118, 317, 344, 425
NmuCI GTSAC 1 cut(s) 124
NspV TTCGAA 1 cut(s) 332
PfeI GAWTC 1 cut(s) 218
PkrI GCNGC 2 cut(s) 204, 400
Psp1406I AACGTT 1 cut(s) 96
PspPI GGNCC 1 cut(s) 326
RseI CAYNNNNRTG 2 cut(s) 107, 113
SatI GCNGC 2 cut(s) 203, 399
Sau3AI GATC 1 cut(s) 129
Sau96I GGNCC 1 cut(s) 326
ScrFI CCNGG 2 cut(s) 41, 42
SetI ASST 8 cut(s) 60, 81, 88, 99, 126, 159, 198, 283
SfuI TTCGAA 1 cut(s) 332
SinI GGWCC 1 cut(s) 326
SmaI CCCGGG 1 cut(s) 42
SmiMI CAYNNNNRTG 2 cut(s) 107, 113
Sse9I AATT 3 cut(s) 334, 358, 367
SsiI CCGC 1 cut(s) 398
SspI AATATT 1 cut(s) 248
StyD4I CCNGG 2 cut(s) 39, 40
TaaI ACNGT 2 cut(s) 254, 326
TaiI ACGT 1 cut(s) 99
TaqI TCGA 3 cut(s) 33, 234, 332
TasI AATT 3 cut(s) 334, 358, 367
TauI GCSGC 1 cut(s) 401
TfiI GAWTC 1 cut(s) 218
TscAI CASTG 3 cut(s) 75, 139, 400
TseFI GTSAC 1 cut(s) 124
TseI GCWGC 1 cut(s) 202
Tsp45I GTSAC 1 cut(s) 124
TspMI CCCGGG 1 cut(s) 40
TspRI CASTG 3 cut(s) 75, 139, 400
VpaK11BI GGWCC 1 cut(s) 326
XapI RAATTY 2 cut(s) 358, 367
XmaI CCCGGG 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.