RchiOBHm_Chr2g0129621

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0014335
Physical Location & Seq
Reverse (-)
45280584 .. 45281179
596 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50125

Sequence Viewer

Length: 444 bp
ATGCAGACGATAGAGATGAGAGTGCATATGGACTGCCCCGGATGCGAGAGCAAGGTTAGAAGTACACTTCAGAAGCTAAAAGGTATCGACAATGTTGAGATAGACATGGCTCTGCAGAAGGTCACAGTGACCGGGTGGGCAGACCAAAAGAAAGTTCTCAAGACGGTTCGAAAAACTGGTCGAAGAGCGGAGCTCTGGCAATTCCCCTTTAATAATTCAAATTCAGACAACAGCGATGTATACAATCAGAGTCAGTGTAATGGTCCGATTAATAACTATGCTCCCCAGCCTTCCTCATCTTACAACTACTACAAGCACGGATATGACAGTGGTCCTTCTTCTAACTATGGCTATTCTCAGAACCCTGCACATTCCTCCGTTTACGGGTATCAACCTACGACGGCCTTCAGTGACGAAAACCCTAATGCTTGTTCTGTAATGTGA

Protein Analysis

147

Amino Acids

16.51

Weight (kDa)

7.65

Isoelectric Point (pI)

44.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 7 - 61 3.5e-15 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014335)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06330
fragaria_vesca FvH4_6g27360 FvH4_6g27360
malus_domestica MD09G1238100.v1.1
prunus_persica Prupe.3G118600_v2.0.a1
pyrus_communis pycom09g15660
rosa_chinensis RchiOBHm_Chr2g0129621
rosa_laevigata RLG00000019090
rosa_multiflora Rmu_sc0011456.1_g000002
rosa_roxburghii Rroxscaffold_2G00114510
rosa_rugosa Rorug02G0287500
rosa_samantha Rh2AG339200 Rh2BG348000 Rh2CG326700 Rh2DG366200
rosa_wichuraiana Rw2G027870 Rw2G028010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 188
AccI GTMKAC 1 cut(s) 240
AciI CCGC 1 cut(s) 188
AcsI RAATTY 1 cut(s) 220
AcuI CTGAAG 2 cut(s) 53, 391
AfaI GTAC 1 cut(s) 64
AfiI CCNNNNNNNGG 1 cut(s) 384
AgsI TTSAA 1 cut(s) 219
AjuI GAANNNNNNNTTGG 2 cut(s) 138, 170
AluBI AGCT 2 cut(s) 76, 193
AluI AGCT 2 cut(s) 76, 193
Alw21I GWGCWC 1 cut(s) 195
AoxI GGCC 1 cut(s) 402
ApoI RAATTY 1 cut(s) 220
ArsI GACNNNNNNTTYG 2 cut(s) 163, 195
AseI ATTAAT 1 cut(s) 270
AspS9I GGNCC 2 cut(s) 263, 332
AsuC2I CCSGG 2 cut(s) 39, 133
AsuII TTCGAA 1 cut(s) 169
AvaII GGWCC 2 cut(s) 263, 332
BanII GRGCYC 1 cut(s) 195
Bbv12I GWGCWC 1 cut(s) 195
BceAI ACGGC 1 cut(s) 417
BcnI CCSGG 2 cut(s) 39, 133
BfmI CTRYAG 1 cut(s) 113
Bme1390I CCNGG 2 cut(s) 39, 133
Bme18I GGWCC 2 cut(s) 263, 332
BmgT120I GGNCC 2 cut(s) 263, 332
BmrFI CCNGG 2 cut(s) 39, 133
BmsI GCATC 1 cut(s) 32
BoxI GACNNNNGTC 1 cut(s) 330
BplI GAGNNNNNCTC 2 cut(s) 177, 209
Bpu14I TTCGAA 1 cut(s) 169
BpuEI CTTGAG 1 cut(s) 143
BpuMI CCSGG 2 cut(s) 39, 133
BsaJI CCNNGG 1 cut(s) 37
Bsc4I CCNNNNNNNGG 1 cut(s) 384
Bse1I ACTGG 1 cut(s) 181
BseDI CCNNGG 1 cut(s) 37
BseGI GGATG 1 cut(s) 47
BseLI CCNNNNNNNGG 1 cut(s) 384
BseMII CTCAG 1 cut(s) 371
BseNI ACTGG 1 cut(s) 181
BseYI CCCAGC 1 cut(s) 285
BsgI GTGCAG 1 cut(s) 351
BshFI GGCC 1 cut(s) 404
BsiHKAI GWGCWC 1 cut(s) 195
BsiSI CCGG 2 cut(s) 39, 132
BslI CCNNNNNNNGG 1 cut(s) 384
BsnI GGCC 1 cut(s) 404
Bsp119I TTCGAA 1 cut(s) 169
Bsp1286I GDGCHC 1 cut(s) 195
BspACI CCGC 1 cut(s) 188
BspANI GGCC 1 cut(s) 404
BspCNI CTCAG 1 cut(s) 370
BspMAI CTGCAG 1 cut(s) 117
BspQI GCTCTTC 1 cut(s) 178
BspT104I TTCGAA 1 cut(s) 169
BsrBI CCGCTC 1 cut(s) 188
BsrI ACTGG 1 cut(s) 181
BssECI CCNNGG 1 cut(s) 37
BssNAI GTATAC 1 cut(s) 241
Bst1107I GTATAC 1 cut(s) 241
Bst4CI ACNGT 3 cut(s) 127, 166, 329
Bst6I CTCTTC 1 cut(s) 178
BstBI TTCGAA 1 cut(s) 169
BstDEI CTNAG 1 cut(s) 357
BstF5I GGATG 1 cut(s) 47
BstMWI GCNNNNNNNGC 1 cut(s) 42
BstPAI GACNNNNGTC 1 cut(s) 330
BstSCI CCNGG 2 cut(s) 37, 131
BstSFI CTRYAG 1 cut(s) 113
BstZ17I GTATAC 1 cut(s) 241
BsuRI GGCC 1 cut(s) 404
BtgZI GCGATG 1 cut(s) 249
BtsCI GGATG 1 cut(s) 47
BtsIMutI CAGTG 4 cut(s) 132, 260, 334, 415
Cfr13I GGNCC 2 cut(s) 263, 332
Csp6I GTAC 1 cut(s) 63
CviAII CATG 1 cut(s) 106
CviJI RGCY 6 cut(s) 76, 110, 193, 289, 351, 404
CviKI_1 RGCY 6 cut(s) 76, 110, 193, 289, 351, 404
CviQI GTAC 1 cut(s) 63
DdeI CTNAG 1 cut(s) 357
Eam1104I CTCTTC 1 cut(s) 178
EarI CTCTTC 1 cut(s) 178
Ecl136II GAGCTC 1 cut(s) 193
Eco24I GRGCYC 1 cut(s) 195
Eco47I GGWCC 2 cut(s) 263, 332
Eco53kI GAGCTC 1 cut(s) 193
Eco57I CTGAAG 2 cut(s) 53, 391
EcoICRI GAGCTC 1 cut(s) 193
EcoT38I GRGCYC 1 cut(s) 195
FaeI CATG 1 cut(s) 109
FaiI YATR 7 cut(s) 27, 29, 107, 241, 279, 324, 348
FatI CATG 1 cut(s) 105
FauNDI CATATG 1 cut(s) 27
FblI GTMKAC 1 cut(s) 240
FokI GGATG 1 cut(s) 54
FriOI GRGCYC 1 cut(s) 195
GsaI CCCAGC 1 cut(s) 289
HaeIII GGCC 1 cut(s) 404
HapII CCGG 2 cut(s) 39, 132
Hin1II CATG 1 cut(s) 109
HinfI GANTC 1 cut(s) 250
HpaII CCGG 2 cut(s) 39, 132
Hpy166II GTNNAC 3 cut(s) 65, 241, 382
Hpy188I TCNGA 5 cut(s) 72, 226, 249, 267, 360
Hpy188III TCNNGA 1 cut(s) 160
Hpy8I GTNNAC 3 cut(s) 65, 241, 382
Hpy99I CGWCG 1 cut(s) 403
HpyAV CCTTC 4 cut(s) 112, 300, 345, 415
HpyCH4III ACNGT 3 cut(s) 127, 166, 329
HpyCH4V TGCA 4 cut(s) 4, 25, 115, 368
HpyF10VI GCNNNNNNNGC 1 cut(s) 42
HpyF3I CTNAG 1 cut(s) 357
Hsp92II CATG 1 cut(s) 109
LguI GCTCTTC 1 cut(s) 178
LmnI GCTCC 2 cut(s) 190, 286
LpnPI CCDG 6 cut(s) 52, 145, 162, 181, 299, 378
LweI GCATC 1 cut(s) 32
MaeIII GTNAC 3 cut(s) 121, 127, 410
MbiI CCGCTC 1 cut(s) 188
MboII GAAGA 2 cut(s) 195, 330
MhlI GDGCHC 1 cut(s) 195
MluCI AATT 3 cut(s) 200, 214, 220
MlyI GAGTC 1 cut(s) 259
MnlI CCTC 2 cut(s) 304, 385
MseI TTAA 2 cut(s) 210, 270
MslI CAYNNNNRTG 1 cut(s) 321
MspI CCGG 2 cut(s) 39, 132
MspR9I CCNGG 2 cut(s) 39, 133
MwoI GCNNNNNNNGC 1 cut(s) 42
NciI CCSGG 2 cut(s) 39, 133
NdeI CATATG 1 cut(s) 27
NlaIII CATG 1 cut(s) 109
NmuCI GTSAC 3 cut(s) 121, 127, 410
NspV TTCGAA 1 cut(s) 169
PciSI GCTCTTC 1 cut(s) 178
PleI GAGTC 1 cut(s) 258
PpsI GAGTC 1 cut(s) 258
PshAI GACNNNNGTC 1 cut(s) 330
PshBI ATTAAT 1 cut(s) 270
Psp124BI GAGCTC 1 cut(s) 195
PspFI CCCAGC 1 cut(s) 285
PspPI GGNCC 2 cut(s) 263, 332
PstI CTGCAG 1 cut(s) 117
RsaI GTAC 1 cut(s) 64
RsaNI GTAC 1 cut(s) 63
RseI CAYNNNNRTG 1 cut(s) 321
SacI GAGCTC 1 cut(s) 195
SapI GCTCTTC 1 cut(s) 178
SaqAI TTAA 2 cut(s) 210, 270
Sau96I GGNCC 2 cut(s) 263, 332
SchI GAGTC 1 cut(s) 259
ScrFI CCNGG 2 cut(s) 39, 133
SduI GDGCHC 1 cut(s) 195
SetI ASST 6 cut(s) 57, 78, 85, 123, 195, 397
SfaNI GCATC 1 cut(s) 32
SfcI CTRYAG 1 cut(s) 113
SfuI TTCGAA 1 cut(s) 169
SinI GGWCC 2 cut(s) 263, 332
SmiMI CAYNNNNRTG 1 cut(s) 321
SmlI CTYRAG 1 cut(s) 158
SmoI CTYRAG 1 cut(s) 158
Sse9I AATT 3 cut(s) 200, 214, 220
SsiI CCGC 1 cut(s) 188
SstI GAGCTC 1 cut(s) 195
StyD4I CCNGG 2 cut(s) 37, 131
TaaI ACNGT 3 cut(s) 127, 166, 329
TaqI TCGA 3 cut(s) 87, 169, 181
TasI AATT 3 cut(s) 200, 214, 220
TatI WGTACW 1 cut(s) 62
Tru1I TTAA 2 cut(s) 210, 270
Tru9I TTAA 2 cut(s) 210, 270
TscAI CASTG 4 cut(s) 132, 260, 334, 415
TseFI GTSAC 3 cut(s) 121, 127, 410
Tsp45I GTSAC 3 cut(s) 121, 127, 410
TspGWI ACGGA 2 cut(s) 333, 367
TspRI CASTG 4 cut(s) 132, 260, 334, 415
VpaK11BI GGWCC 2 cut(s) 263, 332
VspI ATTAAT 1 cut(s) 270
XapI RAATTY 1 cut(s) 220
XmiI GTMKAC 1 cut(s) 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.