MD09G1255000.v1.1

Alpha beta hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0010688
Physical Location & Seq
Reverse (-)
32650316 .. 32655304
4989 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1255000.v1.1.491

Sequence Viewer

Length: 1029 bp
ATGGGCGGAGTGACCTCTTCGATCGCAGCCAAGTTCGCGTTTTTCCCGCCGAGTCCGCCGTCGTACAGAGTCGTCGCCGACGAGTCGTGTGGAGGGAGGCTCTACATTCCGGAGGTGCCGAGGAGGGACGACGTGGATGTGCTGAAGCTCAGGACTCGCCGGGGAAATGAAATCGTGGCCGTACACGTCAAGCACCCCAAGGCCTCAGCAACCATGCTTTACTCCCATGGAAATGCTGCTGATTTGGGGCAGATGTATGAGCTTTTCGTCGAATTGAGTGCTCGCCTTCGGATTAATTTGATGGGGTATGATTACTCTGGTTATGGACAGTCAACTGGAAAGGCAACTGAATATAATACATATGCGGACATTGATGCAGCTTACAAATGCCTCAAGGAGAAATACGGGGTCAAAGATGAACAACTAATATTATATGGTCAGTCTGTTGGTAGTGGTCCCACTGTTGATCTTGCATCACGTCTCGCAACCTTGAGAGGTGTGGTGTTACACAGTCCTATTTTGTCTGGGCTGAGGGTATTGTACCCTGTCAAACGGACGTACTGGTTTGATATTTACAAGAATATCGACAAAATTTCAATGGTGAGCTGTCCTGTTCTGGTTATACATGGGACAGCAGACGAGGTCGTAGATTCGTCCCATGGGAAGCAACTATGGGTGCTTTGCAAGGAAAAGTATGAGCCTTTATGGCTCAGTGGAGGTGGACACTGCAATCTTGAGCTTTACCCAGAATTTATCAAACATCTAAAGAAATTCGTACACAGCCTCAGCAAGTCAAAATCGACCACAAATGGTTCTAGAACAAGTACAGTAGATTCTGAGAACAAGAACAAACCATCTGAAACCGGTACTTCGGATTCAACTGAAGTGGGTTCTGATCTTCCGGAAGTTTCTAGAAACAGTTTAGATAGTCGACTAGAGAAATCTAAAAAGTCGAACAAGCCCGAAAAGTCTCGGATGAGCACTGACCGAGTCGACACGTTTAGGAGAAAGAAGGGATTAGTGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FSH1 Hydrolase_4

Protein Analysis

343

Amino Acids

38.07

Weight (kDa)

9.17

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 67 - 175 2.6e-08 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 115
AccI GTMKAC 2 cut(s) 931, 993
AccII CGCG 1 cut(s) 38
AccIII TCCGGA 2 cut(s) 109, 901
AciI CCGC 4 cut(s) 6, 47, 56, 365
AcoI YGGCCR 1 cut(s) 177
AcsI RAATTY 3 cut(s) 591, 749, 770
AcuI CTGAAG 2 cut(s) 164, 903
AfaI GTAC 7 cut(s) 65, 183, 542, 560, 777, 826, 868
AflIII ACRYGT 2 cut(s) 184, 996
AgeI ACCGGT 1 cut(s) 863
AgsI TTSAA 2 cut(s) 597, 879
AjiI CACGTC 3 cut(s) 133, 187, 479
AluBI AGCT 5 cut(s) 148, 262, 380, 606, 739
AluI AGCT 5 cut(s) 148, 262, 380, 606, 739
Alw21I GWGCWC 2 cut(s) 283, 983
Alw26I GTCTC 2 cut(s) 485, 975
Aor13HI TCCGGA 2 cut(s) 109, 901
AoxI GGCC 2 cut(s) 177, 201
ApeKI GCWGC 3 cut(s) 26, 236, 377
ApoI RAATTY 3 cut(s) 591, 749, 770
AseI ATTAAT 1 cut(s) 294
AsiGI ACCGGT 1 cut(s) 863
AspS9I GGNCC 1 cut(s) 455
AsuC2I CCSGG 1 cut(s) 161
AsuHPI GGTGA 1 cut(s) 613
AvaII GGWCC 1 cut(s) 455
BanI GGYRCC 1 cut(s) 115
Bbv12I GWGCWC 2 cut(s) 283, 983
BbvCI CCTCAGC 3 cut(s) 205, 530, 785
BbvI GCAGC 3 cut(s) 38, 223, 389
BccI CCATC 2 cut(s) 295, 862
BceAI ACGGC 2 cut(s) 43, 164
BcgI CGANNNNNNTGC 2 cut(s) 260, 294
BcnI CCSGG 1 cut(s) 161
BcoDI GTCTC 2 cut(s) 485, 975
BfaI CTAG 3 cut(s) 816, 912, 935
BglI GCCNNNNNGGC 1 cut(s) 706
BisI GCNGC 3 cut(s) 27, 237, 378
BlsI GCNGC 3 cut(s) 28, 238, 379
Bme1390I CCNGG 1 cut(s) 161
Bme18I GGWCC 1 cut(s) 455
BmgBI CACGTC 3 cut(s) 133, 187, 479
BmgT120I GGNCC 1 cut(s) 455
BmiI GGNNCC 2 cut(s) 117, 457
BmrFI CCNGG 1 cut(s) 161
BmsI GCATC 2 cut(s) 364, 482
BplI GAGNNNNNCTC 2 cut(s) 84, 116
Bpu10I CCTNAGC 4 cut(s) 149, 205, 530, 785
BpuEI CTTGAG 3 cut(s) 377, 511, 755
BpuMI CCSGG 1 cut(s) 161
BsaJI CCNNGG 5 cut(s) 119, 160, 198, 226, 658
BsaWI WCCGGW 3 cut(s) 109, 863, 901
Bse118I RCCGGY 1 cut(s) 863
Bse1I ACTGG 2 cut(s) 340, 566
BseAI TCCGGA 2 cut(s) 109, 901
BseDI CCNNGG 5 cut(s) 119, 160, 198, 226, 658
BseGI GGATG 2 cut(s) 142, 981
BseMII CTCAG 6 cut(s) 163, 219, 521, 724, 799, 828
BseNI ACTGG 2 cut(s) 340, 566
BseRI GAGGAG 1 cut(s) 136
BseXI GCAGC 3 cut(s) 38, 223, 389
Bsh1236I CGCG 1 cut(s) 38
Bsh1285I CGRYCG 1 cut(s) 24
BshFI GGCC 2 cut(s) 179, 203
BshNI GGYRCC 1 cut(s) 115
BshTI ACCGGT 1 cut(s) 863
BsiEI CGRYCG 1 cut(s) 24
BsiHKAI GWGCWC 2 cut(s) 283, 983
BsiSI CCGG 4 cut(s) 110, 160, 864, 902
BslFI GGGAC 4 cut(s) 140, 441, 640, 643
BsmAI GTCTC 2 cut(s) 485, 975
BsmBI CGTCTC 1 cut(s) 485
BsmFI GGGAC 4 cut(s) 140, 441, 640, 643
BsnI GGCC 2 cut(s) 179, 203
Bsp1286I GDGCHC 2 cut(s) 283, 983
Bsp13I TCCGGA 2 cut(s) 109, 901
Bsp143I GATC 3 cut(s) 21, 466, 895
Bsp19I CCATGG 2 cut(s) 226, 658
BspACI CCGC 4 cut(s) 6, 47, 56, 365
BspANI GGCC 2 cut(s) 179, 203
BspCNI CTCAG 6 cut(s) 162, 218, 522, 723, 798, 829
BspEI TCCGGA 2 cut(s) 109, 901
BspFNI CGCG 1 cut(s) 38
BspLI GGNNCC 2 cut(s) 117, 457
BspT107I GGYRCC 1 cut(s) 115
BsrFI RCCGGY 1 cut(s) 863
BsrI ACTGG 2 cut(s) 340, 566
BssAI RCCGGY 1 cut(s) 863
BssECI CCNNGG 5 cut(s) 119, 160, 198, 226, 658
BssMI GATC 3 cut(s) 21, 466, 895
BssT1I CCWWGG 3 cut(s) 198, 226, 658
Bst4CI ACNGT 5 cut(s) 330, 463, 512, 829, 920
Bst6I CTCTTC 1 cut(s) 22
BstC8I GCNNGC 1 cut(s) 283
BstDEI CTNAG 6 cut(s) 149, 205, 530, 710, 785, 837
BstDSI CCRYGG 2 cut(s) 226, 658
BstF5I GGATG 2 cut(s) 142, 981
BstFNI CGCG 1 cut(s) 38
BstKTI GATC 3 cut(s) 24, 469, 898
BstMAI GTCTC 2 cut(s) 485, 975
BstMBI GATC 3 cut(s) 21, 466, 895
BstMCI CGRYCG 1 cut(s) 24
BstMWI GCNNNNNNNGC 3 cut(s) 35, 55, 706
BstSCI CCNGG 1 cut(s) 159
BstUI CGCG 1 cut(s) 38
BstV1I GCAGC 3 cut(s) 38, 223, 389
BsuRI GGCC 2 cut(s) 179, 203
BtgI CCRYGG 2 cut(s) 226, 658
BtrI CACGTC 3 cut(s) 133, 187, 479
BtsCI GGATG 2 cut(s) 142, 981
BtsI GCAGTG 1 cut(s) 724
BtsIMutI CAGTG 4 cut(s) 459, 718, 724, 981
Cac8I GCNNGC 1 cut(s) 283
Cfr10I RCCGGY 1 cut(s) 863
Cfr13I GGNCC 1 cut(s) 455
Csp6I GTAC 7 cut(s) 64, 182, 541, 559, 776, 825, 867
CspAI ACCGGT 1 cut(s) 863
CspCI CAANNNNNGTGG 2 cut(s) 867, 902
CviAII CATG 4 cut(s) 214, 227, 626, 659
CviQI GTAC 7 cut(s) 64, 182, 541, 559, 776, 825, 867
DdeI CTNAG 6 cut(s) 149, 205, 530, 710, 785, 837
DpnI GATC 3 cut(s) 23, 468, 897
DpnII GATC 3 cut(s) 21, 466, 895
EaeI YGGCCR 1 cut(s) 177
Eam1104I CTCTTC 1 cut(s) 22
EarI CTCTTC 1 cut(s) 22
EciI GGCGGA 2 cut(s) 21, 45
Eco130I CCWWGG 3 cut(s) 198, 226, 658
Eco147I AGGCCT 1 cut(s) 203
Eco47I GGWCC 1 cut(s) 455
Eco57I CTGAAG 2 cut(s) 164, 903
EcoT14I CCWWGG 3 cut(s) 198, 226, 658
ErhI CCWWGG 3 cut(s) 198, 226, 658
Esp3I CGTCTC 1 cut(s) 485
FaeI CATG 4 cut(s) 217, 230, 629, 662
FaqI GGGAC 4 cut(s) 140, 441, 640, 643
FatI CATG 4 cut(s) 213, 226, 625, 658
FauI CCCGC 1 cut(s) 54
FauNDI CATATG 1 cut(s) 361
FblI GTMKAC 2 cut(s) 931, 993
Fnu4HI GCNGC 3 cut(s) 27, 237, 378
FokI GGATG 2 cut(s) 149, 988
Fsp4HI GCNGC 3 cut(s) 27, 237, 378
FspBI CTAG 3 cut(s) 816, 912, 935
GluI GCNGC 3 cut(s) 27, 237, 378
HaeIII GGCC 2 cut(s) 179, 203
HapII CCGG 4 cut(s) 110, 160, 864, 902
Hin1II CATG 4 cut(s) 217, 230, 629, 662
HincII GTYRAC 3 cut(s) 333, 932, 994
HindII GTYRAC 3 cut(s) 333, 932, 994
HinfI GANTC 8 cut(s) 52, 69, 83, 154, 650, 833, 875, 990
HpaII CCGG 4 cut(s) 110, 160, 864, 902
HphI GGTGA 1 cut(s) 613
Hpy166II GTNNAC 6 cut(s) 184, 333, 722, 778, 932, 994
Hpy188I TCNGA 6 cut(s) 291, 838, 859, 874, 895, 975
Hpy188III TCNNGA 6 cut(s) 110, 151, 734, 816, 902, 912
Hpy8I GTNNAC 6 cut(s) 184, 333, 722, 778, 932, 994
Hpy99I CGWCG 5 cut(s) 64, 77, 83, 134, 272
HpyAV CCTTC 2 cut(s) 296, 1006
HpyCH4III ACNGT 5 cut(s) 330, 463, 512, 829, 920
HpyCH4IV ACGT 5 cut(s) 132, 186, 478, 557, 998
HpyCH4V TGCA 4 cut(s) 377, 473, 684, 729
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 55, 706
HpyF3I CTNAG 6 cut(s) 149, 205, 530, 710, 785, 837
HpySE526I ACGT 5 cut(s) 132, 186, 478, 557, 998
Hsp92II CATG 4 cut(s) 217, 230, 629, 662
Kpn2I TCCGGA 2 cut(s) 109, 901
Kzo9I GATC 3 cut(s) 21, 466, 895
Lsp1109I GCAGC 3 cut(s) 38, 223, 389
LweI GCATC 2 cut(s) 364, 482
MaeI CTAG 3 cut(s) 816, 912, 935
MaeII ACGT 5 cut(s) 132, 186, 478, 557, 998
MaeIII GTNAC 2 cut(s) 10, 504
MalI GATC 3 cut(s) 23, 468, 897
MboI GATC 3 cut(s) 21, 466, 895
MboII GAAGA 2 cut(s) 9, 890
MhlI GDGCHC 2 cut(s) 283, 983
MluCI AATT 5 cut(s) 272, 295, 591, 749, 770
MlyI GAGTC 5 cut(s) 61, 78, 92, 148, 999
MroI TCCGGA 2 cut(s) 109, 901
MseI TTAA 1 cut(s) 294
MslI CAYNNNNRTG 1 cut(s) 231
MspI CCGG 4 cut(s) 110, 160, 864, 902
MspR9I CCNGG 1 cut(s) 161
MvnI CGCG 1 cut(s) 38
MwoI GCNNNNNNNGC 3 cut(s) 35, 55, 706
NciI CCSGG 1 cut(s) 161
NcoI CCATGG 2 cut(s) 226, 658
NdeI CATATG 1 cut(s) 361
NdeII GATC 3 cut(s) 21, 466, 895
NlaIII CATG 4 cut(s) 217, 230, 629, 662
NlaIV GGNNCC 2 cut(s) 117, 457
NmeAIII GCCGAG 2 cut(s) 75, 144
NmuCI GTSAC 1 cut(s) 10
PceI AGGCCT 1 cut(s) 203
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PfeI GAWTC 3 cut(s) 650, 833, 875
PflFI GACNNNGTC 2 cut(s) 641, 989
PinAI ACCGGT 1 cut(s) 863
PkrI GCNGC 3 cut(s) 28, 238, 379
Ple19I CGATCG 1 cut(s) 24
PleI GAGTC 5 cut(s) 60, 77, 91, 148, 998
PpsI GAGTC 5 cut(s) 60, 77, 91, 148, 998
PshBI ATTAAT 1 cut(s) 294
PspN4I GGNNCC 2 cut(s) 117, 457
PspPI GGNCC 1 cut(s) 455
PsyI GACNNNGTC 2 cut(s) 641, 989
PvuI CGATCG 1 cut(s) 24
RsaI GTAC 7 cut(s) 65, 183, 542, 560, 777, 826, 868
RsaNI GTAC 7 cut(s) 64, 182, 541, 559, 776, 825, 867
RseI CAYNNNNRTG 1 cut(s) 231
SalI GTCGAC 2 cut(s) 930, 992
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 3 cut(s) 27, 237, 378
Sau3AI GATC 3 cut(s) 21, 466, 895
Sau96I GGNCC 1 cut(s) 455
SchI GAGTC 5 cut(s) 61, 78, 92, 148, 999
ScrFI CCNGG 1 cut(s) 161
SduI GDGCHC 2 cut(s) 283, 983
SfaNI GCATC 2 cut(s) 364, 482
SinI GGWCC 1 cut(s) 455
SmiMI CAYNNNNRTG 1 cut(s) 231
SmlI CTYRAG 3 cut(s) 392, 490, 734
SmoI CTYRAG 3 cut(s) 392, 490, 734
Sse9I AATT 5 cut(s) 272, 295, 591, 749, 770
SseBI AGGCCT 1 cut(s) 203
SsiI CCGC 4 cut(s) 6, 47, 56, 365
SspI AATATT 1 cut(s) 429
SspMI CTAG 3 cut(s) 816, 912, 935
StuI AGGCCT 1 cut(s) 203
StyD4I CCNGG 1 cut(s) 159
StyI CCWWGG 3 cut(s) 198, 226, 658
TaaI ACNGT 5 cut(s) 330, 463, 512, 829, 920
TaiI ACGT 5 cut(s) 135, 189, 481, 560, 1001
TaqI TCGA 7 cut(s) 20, 270, 585, 800, 931, 953, 993
TaqII GACCGA 1 cut(s) 1002
TasI AATT 5 cut(s) 272, 295, 591, 749, 770
TatI WGTACW 1 cut(s) 824
TfiI GAWTC 3 cut(s) 650, 833, 875
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TscAI CASTG 4 cut(s) 466, 718, 731, 988
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 3 cut(s) 26, 236, 377
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 2 cut(s) 183, 432
TspGWI ACGGA 1 cut(s) 568
TspRI CASTG 4 cut(s) 466, 718, 731, 988
Tth111I GACNNNGTC 2 cut(s) 641, 989
VpaK11BI GGWCC 1 cut(s) 455
VspI ATTAAT 1 cut(s) 294
XapI RAATTY 3 cut(s) 591, 749, 770
XbaI TCTAGA 2 cut(s) 815, 911
XmiI GTMKAC 2 cut(s) 931, 993
XspI CTAG 3 cut(s) 816, 912, 935
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.