Rw0G015010

Alpha beta hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00679
OG0010688
Physical Location & Seq
Reverse (-)
11256 .. 15589
4334 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G015010.1

Sequence Viewer

Length: 735 bp
ATGGAATACCGGTATGATTACTCTGGCTATGGGCAGTCAACTGGAAAGGCATCTGAATGTAATACATACGCAGATATTGATGCAGCATATAAATGCCTCAAGGAGCAGTGTGGGGTCAAGGATGAACAACTGATATTATATGGTCAGTCTGTTGGTAGTGGCCCCACTGTTGATCTTGCTTCACGTATACCAACCTTGAGAGGTGTAGTGTTACACAGTCCCATTATGTCCGGGTTGAGGGTGTTGTACCCTGTCAAGCGGACATACTGGTTTGATATTTACAAGAATATCGATAAAATTGCAATGGTGAACTGCCCTATCCTGGTTATACATGGGACAGCAGATGAAGTTGTAGATTCCTCCCATGGCAAGCAGCTTTGGGTTCTTTGCAAGGAAAAGTATGAACCTTTGTGGATTAGTGGAGGTGGACATTGCAATCTTGAGCTTTACCCAGAATTTATTAAACATCTAAAGAAATTTGTACAAAGTCTTGGCAAGTCAAAATCTGCCACAGGTGGTTCAAAAAAAAGTACAGTTGATTCTGATGGCCAAAGCAAAAATTCTGAAACAGGTACTTCAGATTCGCTTGAATTGGCTGCAGATCTATCAGAAGTTTCTAGAAACAGTTTAGATAGTCGACTTGAGAAGTCTAAAAAGTCTAACAAACCTGAGAAGTCTCGAATGAGCACCGACCAAGTTGACACCTTTAGAAGAAAAAAGGGTTTGGTGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FSH1 Hydrolase_4

Protein Analysis

244

Amino Acids

27.16

Weight (kDa)

8.74

Isoelectric Point (pI)

43.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 3 - 77 1.9e-06 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 187, 637
AciI CCGC 1 cut(s) 259
AcoI YGGCCR 1 cut(s) 547
AcsI RAATTY 3 cut(s) 455, 476, 559
AcuI CTGAAG 1 cut(s) 561
AfaI GTAC 4 cut(s) 248, 483, 532, 574
AfiI CCNNNNNNNGG 2 cut(s) 237, 322
AgeI ACCGGT 1 cut(s) 9
AgsI TTSAA 2 cut(s) 522, 590
AjnI CCWGG 1 cut(s) 321
AluBI AGCT 2 cut(s) 376, 445
AluI AGCT 2 cut(s) 376, 445
Alw21I GWGCWC 1 cut(s) 689
Alw26I GTCTC 1 cut(s) 681
AoxI GGCC 2 cut(s) 160, 547
ApeKI GCWGC 3 cut(s) 83, 373, 596
ApoI RAATTY 3 cut(s) 455, 476, 559
AsiGI ACCGGT 1 cut(s) 9
AspS9I GGNCC 1 cut(s) 161
AsuC2I CCSGG 1 cut(s) 232
AsuHPI GGTGA 1 cut(s) 319
BalI TGGCCA 1 cut(s) 549
Bbv12I GWGCWC 1 cut(s) 689
BbvI GCAGC 3 cut(s) 95, 385, 583
BccI CCATC 1 cut(s) 539
BcgI CGANNNNNNTGC 2 cut(s) 281, 315
BciT130I CCWGG 1 cut(s) 323
BcnI CCSGG 1 cut(s) 232
BcoDI GTCTC 1 cut(s) 681
BfaI CTAG 1 cut(s) 618
BfmI CTRYAG 1 cut(s) 597
BglII AGATCT 1 cut(s) 601
BisI GCNGC 3 cut(s) 84, 374, 597
BlsI GCNGC 3 cut(s) 85, 375, 598
Bme1390I CCNGG 2 cut(s) 232, 323
BmgT120I GGNCC 1 cut(s) 161
BmiI GGNNCC 1 cut(s) 163
BmrFI CCNGG 2 cut(s) 232, 323
BmsI GCATC 2 cut(s) 59, 70
BpuEI CTTGAG 4 cut(s) 83, 217, 461, 662
BpuMI CCSGG 1 cut(s) 232
Bsa29I ATCGAT 1 cut(s) 291
BsaAI YACGTR 1 cut(s) 185
BsaJI CCNNGG 1 cut(s) 364
BsaWI WCCGGW 1 cut(s) 9
Bsc4I CCNNNNNNNGG 2 cut(s) 237, 322
Bse118I RCCGGY 1 cut(s) 9
Bse1I ACTGG 2 cut(s) 46, 272
Bse3DI GCAATG 2 cut(s) 309, 430
BseBI CCWGG 1 cut(s) 323
BseCI ATCGAT 1 cut(s) 291
BseDI CCNNGG 1 cut(s) 364
BseGI GGATG 1 cut(s) 127
BseLI CCNNNNNNNGG 2 cut(s) 237, 322
BseMI GCAATG 2 cut(s) 309, 430
BseMII CTCAG 1 cut(s) 660
BseNI ACTGG 2 cut(s) 46, 272
BseXI GCAGC 3 cut(s) 95, 385, 583
BshFI GGCC 2 cut(s) 162, 549
BshTI ACCGGT 1 cut(s) 9
BshVI ATCGAT 1 cut(s) 291
BsiHKAI GWGCWC 1 cut(s) 689
BsiSI CCGG 2 cut(s) 10, 231
BslFI GGGAC 2 cut(s) 204, 349
BslI CCNNNNNNNGG 2 cut(s) 237, 322
BsmAI GTCTC 1 cut(s) 681
BsmFI GGGAC 2 cut(s) 204, 349
BsnI GGCC 2 cut(s) 162, 549
Bsp1286I GDGCHC 1 cut(s) 689
Bsp1407I TGTACA 1 cut(s) 481
Bsp143I GATC 2 cut(s) 172, 601
Bsp19I CCATGG 1 cut(s) 364
BspACI CCGC 1 cut(s) 259
BspANI GGCC 2 cut(s) 162, 549
BspCNI CTCAG 1 cut(s) 661
BspDI ATCGAT 1 cut(s) 291
BspLI GGNNCC 1 cut(s) 163
BspMAI CTGCAG 1 cut(s) 601
BsrDI GCAATG 2 cut(s) 309, 430
BsrFI RCCGGY 1 cut(s) 9
BsrGI TGTACA 1 cut(s) 481
BsrI ACTGG 2 cut(s) 46, 272
BssAI RCCGGY 1 cut(s) 9
BssECI CCNNGG 1 cut(s) 364
BssMI GATC 2 cut(s) 172, 601
BssNAI GTATAC 1 cut(s) 188
BssT1I CCWWGG 1 cut(s) 364
Bst1107I GTATAC 1 cut(s) 188
Bst2UI CCWGG 1 cut(s) 323
Bst4CI ACNGT 4 cut(s) 169, 218, 535, 626
BstAUI TGTACA 1 cut(s) 481
BstBAI YACGTR 1 cut(s) 185
BstC8I GCNNGC 1 cut(s) 371
BstDEI CTNAG 1 cut(s) 669
BstDSI CCRYGG 1 cut(s) 364
BstF5I GGATG 1 cut(s) 127
BstKTI GATC 2 cut(s) 175, 604
BstMAI GTCTC 1 cut(s) 681
BstMBI GATC 2 cut(s) 172, 601
BstNI CCWGG 1 cut(s) 323
BstSCI CCNGG 2 cut(s) 230, 321
BstSFI CTRYAG 1 cut(s) 597
BstV1I GCAGC 3 cut(s) 95, 385, 583
BstX2I RGATCY 1 cut(s) 601
BstYI RGATCY 1 cut(s) 601
BstZ17I GTATAC 1 cut(s) 188
Bsu15I ATCGAT 1 cut(s) 291
BsuRI GGCC 2 cut(s) 162, 549
BsuTUI ATCGAT 1 cut(s) 291
BtgI CCRYGG 1 cut(s) 364
BtsCI GGATG 1 cut(s) 127
BtsI GCAGTG 1 cut(s) 113
BtsIMutI CAGTG 2 cut(s) 113, 165
Cac8I GCNNGC 1 cut(s) 371
Cfr10I RCCGGY 1 cut(s) 9
Cfr13I GGNCC 1 cut(s) 161
ClaI ATCGAT 1 cut(s) 291
Csp6I GTAC 4 cut(s) 247, 482, 531, 573
CspAI ACCGGT 1 cut(s) 9
CviAII CATG 2 cut(s) 332, 365
CviJI RGCY 6 cut(s) 27, 162, 376, 445, 549, 596
CviKI_1 RGCY 6 cut(s) 27, 162, 376, 445, 549, 596
CviQI GTAC 4 cut(s) 247, 482, 531, 573
DdeI CTNAG 1 cut(s) 669
DpnI GATC 2 cut(s) 174, 603
DpnII GATC 2 cut(s) 172, 601
EaeI YGGCCR 1 cut(s) 547
Eco130I CCWWGG 1 cut(s) 364
Eco57I CTGAAG 1 cut(s) 561
EcoRII CCWGG 1 cut(s) 321
EcoT14I CCWWGG 1 cut(s) 364
ErhI CCWWGG 1 cut(s) 364
FaeI CATG 2 cut(s) 335, 368
FaqI GGGAC 2 cut(s) 204, 349
FatI CATG 2 cut(s) 331, 364
FblI GTMKAC 2 cut(s) 187, 637
Fnu4HI GCNGC 3 cut(s) 84, 374, 597
FokI GGATG 1 cut(s) 134
Fsp4HI GCNGC 3 cut(s) 84, 374, 597
FspBI CTAG 1 cut(s) 618
GluI GCNGC 3 cut(s) 84, 374, 597
HaeIII GGCC 2 cut(s) 162, 549
HapII CCGG 2 cut(s) 10, 231
Hin1II CATG 2 cut(s) 335, 368
HincII GTYRAC 3 cut(s) 39, 638, 700
HindII GTYRAC 3 cut(s) 39, 638, 700
HinfI GANTC 3 cut(s) 356, 539, 581
HpaII CCGG 2 cut(s) 10, 231
HphI GGTGA 1 cut(s) 319
Hpy166II GTNNAC 6 cut(s) 39, 188, 310, 428, 638, 700
Hpy188I TCNGA 5 cut(s) 55, 544, 565, 580, 610
Hpy188III TCNNGA 3 cut(s) 440, 618, 678
Hpy8I GTNNAC 6 cut(s) 39, 188, 310, 428, 638, 700
HpyCH4III ACNGT 4 cut(s) 169, 218, 535, 626
HpyCH4IV ACGT 1 cut(s) 184
HpyCH4V TGCA 5 cut(s) 83, 302, 390, 435, 599
HpyF3I CTNAG 1 cut(s) 669
HpySE526I ACGT 1 cut(s) 184
Hsp92II CATG 2 cut(s) 335, 368
Kzo9I GATC 2 cut(s) 172, 601
LmnI GCTCC 1 cut(s) 103
Lsp1109I GCAGC 3 cut(s) 95, 385, 583
LweI GCATC 2 cut(s) 59, 70
MaeI CTAG 1 cut(s) 618
MaeII ACGT 1 cut(s) 184
MaeIII GTNAC 1 cut(s) 210
MalI GATC 2 cut(s) 174, 603
MboI GATC 2 cut(s) 172, 601
MboII GAAGA 1 cut(s) 723
MflI RGATCY 1 cut(s) 601
MhlI GDGCHC 1 cut(s) 689
MlsI TGGCCA 1 cut(s) 549
MluCI AATT 5 cut(s) 297, 455, 476, 559, 590
MluNI TGGCCA 1 cut(s) 549
MnlI CCTC 5 cut(s) 107, 194, 231, 370, 416
Mox20I TGGCCA 1 cut(s) 549
MscI TGGCCA 1 cut(s) 549
MseI TTAA 1 cut(s) 462
MslI CAYNNNNRTG 2 cut(s) 55, 91
Msp20I TGGCCA 1 cut(s) 549
MspI CCGG 2 cut(s) 10, 231
MspR9I CCNGG 2 cut(s) 232, 323
MvaI CCWGG 1 cut(s) 323
NciI CCSGG 1 cut(s) 232
NcoI CCATGG 1 cut(s) 364
NdeII GATC 2 cut(s) 172, 601
NlaIII CATG 2 cut(s) 335, 368
NlaIV GGNNCC 1 cut(s) 163
PfeI GAWTC 3 cut(s) 356, 539, 581
PinAI ACCGGT 1 cut(s) 9
PkrI GCNGC 3 cut(s) 85, 375, 598
Ppu21I YACGTR 1 cut(s) 185
Psp6I CCWGG 1 cut(s) 321
PspGI CCWGG 1 cut(s) 321
PspN4I GGNNCC 1 cut(s) 163
PspPI GGNCC 1 cut(s) 161
PstI CTGCAG 1 cut(s) 601
PsuI RGATCY 1 cut(s) 601
RsaI GTAC 4 cut(s) 248, 483, 532, 574
RsaNI GTAC 4 cut(s) 247, 482, 531, 573
RseI CAYNNNNRTG 2 cut(s) 55, 91
SalI GTCGAC 1 cut(s) 636
SaqAI TTAA 1 cut(s) 462
SatI GCNGC 3 cut(s) 84, 374, 597
Sau3AI GATC 2 cut(s) 172, 601
Sau96I GGNCC 1 cut(s) 161
ScrFI CCNGG 2 cut(s) 232, 323
SduI GDGCHC 1 cut(s) 689
SfaNI GCATC 2 cut(s) 59, 70
SfcI CTRYAG 1 cut(s) 597
SmiMI CAYNNNNRTG 2 cut(s) 55, 91
SmlI CTYRAG 4 cut(s) 98, 196, 440, 641
SmoI CTYRAG 4 cut(s) 98, 196, 440, 641
Sse9I AATT 5 cut(s) 297, 455, 476, 559, 590
SsiI CCGC 1 cut(s) 259
SspMI CTAG 1 cut(s) 618
StyD4I CCNGG 2 cut(s) 230, 321
StyI CCWWGG 1 cut(s) 364
TaaI ACNGT 4 cut(s) 169, 218, 535, 626
TaiI ACGT 1 cut(s) 187
TaqI TCGA 3 cut(s) 291, 637, 679
TasI AATT 5 cut(s) 297, 455, 476, 559, 590
TatI WGTACW 2 cut(s) 481, 530
TfiI GAWTC 3 cut(s) 356, 539, 581
Tru1I TTAA 1 cut(s) 462
Tru9I TTAA 1 cut(s) 462
TscAI CASTG 2 cut(s) 113, 172
TseI GCWGC 3 cut(s) 83, 373, 596
TspDTI ATGAA 3 cut(s) 138, 360, 417
TspRI CASTG 2 cut(s) 113, 172
XapI RAATTY 3 cut(s) 455, 476, 559
XbaI TCTAGA 1 cut(s) 617
XmiI GTMKAC 2 cut(s) 187, 637
XspI CTAG 1 cut(s) 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.