Rw3G027810

Alpha beta hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0010688
Physical Location & Seq
Reverse (-)
40281531 .. 40289345
7815 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G027810.1

Sequence Viewer

Length: 1029 bp
ATGGGAGGAGTGACGTCTTCAATCGCAGCCAAGTTCGCCTTCTTCCCGCCGAATCCACCGTCGTACAGAGTCATCGCAGACGATTCCTGCGGAGGAAGACTCTACATTCCTGAGGTTCCTAGAAGGGACGACGTGGATATCCTCAAGCTCAGGACTCGCCGTGGCAATGAAATCGTCGCCGTCCACGTCAAGCACCCCAAGGCTTCTGCCACTATGCTTTATTCCCATGGAAATGCTGCTGATTTGGGCCAGATGTTCGAGCTTTTTGTTGAATTGAGCGCTCGCCTTCGGGTTAATTTAATGGGGTATGATTATTCTGGCTATGGGCAGTCAACTGGAAAGGCATCTGAATGTAATACATACGCAGATATTGATGCAGCATATAAATGCCTCAAGGAGCAGTGTGGGGTCAAGGATGAACAACTGATATTATATGGTCAGTCTGTTGGTAGTGGCCCCACTGTTGATCTTGCTTCACGTATACCAACCTTGAGAGGTGTAGTGTTACACAGTCCCATTATGTCCGGGTTGAGGGTGTTGTACCCTGTCAAGCGGACATACTGGTTTGATATTTACAAGAATATCGATAAAATTGCAATGGTGAACTGCCCTATCCTGGTTATACATGGGACAGCAGATGAAGTTGTAGATTCCTCCCATGGCAAGCAGCTTTGGGTTCTTTGCAAGGAAAAGTATGAACCTTTGTGGATTAGTGGAGGTGGACATTGCAATCTTGAGCTTTACCCAGAATTTATTAAACATCTAAAGAAATTTGTACAAAGTCTTGGCAAGTCAAAATCCGCCACAGATGGTTCAAAAAAAAGTACAGTTGATTCTGATGGCCAAAGCAAAAATTCTGAAACAGGTACTTCAGATTCGCTTGAATTGGCTGCAGATCTATCAGAAGTTTCTAGAAACAGTTTAGATAGTCGACTTGAGAAGTCTAAAAGGTCTAACAAACCTGAGAAGTCTCGAATGAGCACCGACCGAGTTGACACCTTTAGAAGAAAAAAGGGTTTGGTGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FSH1 Hydrolase_4

Protein Analysis

342

Amino Acids

37.91

Weight (kDa)

8.97

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 67 - 175 1.4e-08 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 17
AccI GTMKAC 2 cut(s) 481, 931
AciI CCGC 4 cut(s) 47, 90, 553, 801
AcoI YGGCCR 1 cut(s) 841
AcsI RAATTY 3 cut(s) 749, 770, 853
AcuI CTGAAG 1 cut(s) 855
AcyI GRCGYC 1 cut(s) 14
AfaI GTAC 5 cut(s) 65, 542, 777, 826, 868
AfeI AGCGCT 1 cut(s) 280
AfiI CCNNNNNNNGG 2 cut(s) 531, 616
AgsI TTSAA 4 cut(s) 21, 272, 816, 884
AjiI CACGTC 2 cut(s) 133, 187
AjnI CCWGG 1 cut(s) 615
AjuI GAANNNNNNNTTGG 2 cut(s) 23, 55
AluBI AGCT 4 cut(s) 148, 262, 670, 739
AluI AGCT 4 cut(s) 148, 262, 670, 739
Alw21I GWGCWC 1 cut(s) 983
Alw26I GTCTC 1 cut(s) 975
Aor51HI AGCGCT 1 cut(s) 280
AoxI GGCC 3 cut(s) 247, 454, 841
ApeKI GCWGC 5 cut(s) 26, 236, 377, 667, 890
ApoI RAATTY 3 cut(s) 749, 770, 853
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
AspLEI GCGC 1 cut(s) 281
AspS9I GGNCC 2 cut(s) 247, 455
AsuC2I CCSGG 1 cut(s) 526
AsuHPI GGTGA 1 cut(s) 613
AxyI CCTNAGG 1 cut(s) 111
BalI TGGCCA 1 cut(s) 843
BbsI GAAGAC 2 cut(s) 9, 103
Bbv12I GWGCWC 1 cut(s) 983
BbvI GCAGC 5 cut(s) 38, 223, 389, 679, 877
BccI CCATC 2 cut(s) 803, 833
BceAI ACGGC 2 cut(s) 144, 164
BcgI CGANNNNNNTGC 4 cut(s) 154, 188, 575, 609
BciT130I CCWGG 1 cut(s) 617
BcnI CCSGG 1 cut(s) 526
BcoDI GTCTC 1 cut(s) 975
BfaI CTAG 2 cut(s) 120, 912
BfmI CTRYAG 1 cut(s) 891
BfoI RGCGCY 1 cut(s) 282
BglII AGATCT 1 cut(s) 895
BisI GCNGC 5 cut(s) 27, 237, 378, 668, 891
BlsI GCNGC 5 cut(s) 28, 238, 379, 669, 892
Bme1390I CCNGG 2 cut(s) 526, 617
BmgBI CACGTC 2 cut(s) 133, 187
BmgT120I GGNCC 2 cut(s) 247, 455
BmiI GGNNCC 2 cut(s) 117, 457
BmrFI CCNGG 2 cut(s) 526, 617
BmsI GCATC 2 cut(s) 353, 364
BpiI GAAGAC 2 cut(s) 9, 103
BplI GAGNNNNNCTC 2 cut(s) 84, 116
Bpu10I CCTNAGC 1 cut(s) 149
BpuEI CTTGAG 5 cut(s) 128, 377, 511, 755, 956
BpuMI CCSGG 1 cut(s) 526
Bsa29I ATCGAT 1 cut(s) 585
BsaAI YACGTR 1 cut(s) 479
BsaHI GRCGYC 1 cut(s) 14
BsaJI CCNNGG 4 cut(s) 160, 198, 226, 658
Bsc4I CCNNNNNNNGG 2 cut(s) 531, 616
Bse1I ACTGG 2 cut(s) 340, 566
Bse21I CCTNAGG 1 cut(s) 111
Bse3DI GCAATG 3 cut(s) 172, 603, 724
BseBI CCWGG 1 cut(s) 617
BseCI ATCGAT 1 cut(s) 585
BseDI CCNNGG 4 cut(s) 160, 198, 226, 658
BseGI GGATG 1 cut(s) 421
BseLI CCNNNNNNNGG 2 cut(s) 531, 616
BseMI GCAATG 3 cut(s) 172, 603, 724
BseMII CTCAG 3 cut(s) 102, 163, 954
BseNI ACTGG 2 cut(s) 340, 566
BseRI GAGGAG 1 cut(s) 21
BseXI GCAGC 5 cut(s) 38, 223, 389, 679, 877
Bsh1285I CGRYCG 1 cut(s) 988
BshFI GGCC 3 cut(s) 249, 456, 843
BshVI ATCGAT 1 cut(s) 585
BsiEI CGRYCG 1 cut(s) 988
BsiHKAI GWGCWC 1 cut(s) 983
BsiSI CCGG 1 cut(s) 525
BslFI GGGAC 3 cut(s) 140, 498, 643
BslI CCNNNNNNNGG 2 cut(s) 531, 616
BsmAI GTCTC 1 cut(s) 975
BsmFI GGGAC 3 cut(s) 140, 498, 643
BsnI GGCC 3 cut(s) 249, 456, 843
Bsp1286I GDGCHC 1 cut(s) 983
Bsp1407I TGTACA 1 cut(s) 775
Bsp143I GATC 2 cut(s) 466, 895
Bsp19I CCATGG 2 cut(s) 226, 658
BspACI CCGC 4 cut(s) 47, 90, 553, 801
BspANI GGCC 3 cut(s) 249, 456, 843
BspCNI CTCAG 3 cut(s) 103, 162, 955
BspDI ATCGAT 1 cut(s) 585
BspLI GGNNCC 2 cut(s) 117, 457
BspMAI CTGCAG 1 cut(s) 895
BsrDI GCAATG 3 cut(s) 172, 603, 724
BsrGI TGTACA 1 cut(s) 775
BsrI ACTGG 2 cut(s) 340, 566
BssECI CCNNGG 4 cut(s) 160, 198, 226, 658
BssMI GATC 2 cut(s) 466, 895
BssNAI GTATAC 1 cut(s) 482
BssNI GRCGYC 1 cut(s) 14
BssT1I CCWWGG 3 cut(s) 198, 226, 658
Bst1107I GTATAC 1 cut(s) 482
Bst2UI CCWGG 1 cut(s) 617
Bst4CI ACNGT 5 cut(s) 60, 463, 512, 829, 920
BstACI GRCGYC 1 cut(s) 14
BstAUI TGTACA 1 cut(s) 775
BstBAI YACGTR 1 cut(s) 479
BstC8I GCNNGC 2 cut(s) 283, 665
BstDEI CTNAG 3 cut(s) 111, 149, 963
BstDSI CCRYGG 3 cut(s) 160, 226, 658
BstF5I GGATG 1 cut(s) 421
BstH2I RGCGCY 1 cut(s) 282
BstHHI GCGC 1 cut(s) 281
BstKTI GATC 2 cut(s) 469, 898
BstMAI GTCTC 1 cut(s) 975
BstMBI GATC 2 cut(s) 466, 895
BstMCI CGRYCG 1 cut(s) 988
BstMWI GCNNNNNNNGC 1 cut(s) 35
BstNI CCWGG 1 cut(s) 617
BstSCI CCNGG 2 cut(s) 524, 615
BstSFI CTRYAG 1 cut(s) 891
BstV1I GCAGC 5 cut(s) 38, 223, 389, 679, 877
BstV2I GAAGAC 2 cut(s) 9, 103
BstX2I RGATCY 1 cut(s) 895
BstYI RGATCY 1 cut(s) 895
BstZ17I GTATAC 1 cut(s) 482
Bsu15I ATCGAT 1 cut(s) 585
Bsu36I CCTNAGG 1 cut(s) 111
BsuRI GGCC 3 cut(s) 249, 456, 843
BsuTUI ATCGAT 1 cut(s) 585
BtgI CCRYGG 3 cut(s) 160, 226, 658
BtgZI GCGATG 1 cut(s) 58
BtrI CACGTC 2 cut(s) 133, 187
BtsCI GGATG 1 cut(s) 421
BtsI GCAGTG 1 cut(s) 407
BtsIMutI CAGTG 2 cut(s) 407, 459
Cac8I GCNNGC 2 cut(s) 283, 665
CfoI GCGC 1 cut(s) 281
Cfr13I GGNCC 2 cut(s) 247, 455
ClaI ATCGAT 1 cut(s) 585
Csp6I GTAC 5 cut(s) 64, 541, 776, 825, 867
CviAII CATG 3 cut(s) 227, 626, 659
CviQI GTAC 5 cut(s) 64, 541, 776, 825, 867
DdeI CTNAG 3 cut(s) 111, 149, 963
DpnI GATC 2 cut(s) 468, 897
DpnII GATC 2 cut(s) 466, 895
EaeI YGGCCR 1 cut(s) 841
EciI GGCGGA 1 cut(s) 790
Eco130I CCWWGG 3 cut(s) 198, 226, 658
Eco32I GATATC 1 cut(s) 139
Eco47III AGCGCT 1 cut(s) 280
Eco57I CTGAAG 1 cut(s) 855
Eco81I CCTNAGG 1 cut(s) 111
EcoRII CCWGG 1 cut(s) 615
EcoRV GATATC 1 cut(s) 139
EcoT14I CCWWGG 3 cut(s) 198, 226, 658
ErhI CCWWGG 3 cut(s) 198, 226, 658
FaeI CATG 3 cut(s) 230, 629, 662
FalI AAGNNNNNCTT 2 cut(s) 23, 55
FaqI GGGAC 3 cut(s) 140, 498, 643
FatI CATG 3 cut(s) 226, 625, 658
FauI CCCGC 1 cut(s) 54
FblI GTMKAC 2 cut(s) 481, 931
Fnu4HI GCNGC 5 cut(s) 27, 237, 378, 668, 891
FokI GGATG 1 cut(s) 428
Fsp4HI GCNGC 5 cut(s) 27, 237, 378, 668, 891
FspBI CTAG 2 cut(s) 120, 912
GlaI GCGC 1 cut(s) 280
GluI GCNGC 5 cut(s) 27, 237, 378, 668, 891
HaeII RGCGCY 1 cut(s) 282
HaeIII GGCC 3 cut(s) 249, 456, 843
HapII CCGG 1 cut(s) 525
HhaI GCGC 1 cut(s) 281
Hin1I GRCGYC 1 cut(s) 14
Hin1II CATG 3 cut(s) 230, 629, 662
Hin6I GCGC 1 cut(s) 279
HinP1I GCGC 1 cut(s) 279
HincII GTYRAC 3 cut(s) 333, 932, 994
HindII GTYRAC 3 cut(s) 333, 932, 994
HinfI GANTC 8 cut(s) 52, 69, 83, 99, 154, 650, 833, 875
HpaII CCGG 1 cut(s) 525
HphI GGTGA 1 cut(s) 613
Hpy166II GTNNAC 7 cut(s) 184, 333, 482, 604, 722, 932, 994
Hpy188I TCNGA 5 cut(s) 349, 838, 859, 874, 904
Hpy188III TCNNGA 5 cut(s) 110, 151, 734, 912, 972
Hpy8I GTNNAC 7 cut(s) 184, 333, 482, 604, 722, 932, 994
Hpy99I CGWCG 3 cut(s) 64, 134, 179
HpyAV CCTTC 3 cut(s) 49, 117, 296
HpyCH4III ACNGT 5 cut(s) 60, 463, 512, 829, 920
HpyCH4IV ACGT 4 cut(s) 14, 132, 186, 478
HpyCH4V TGCA 5 cut(s) 377, 596, 684, 729, 893
HpyF10VI GCNNNNNNNGC 1 cut(s) 35
HpyF3I CTNAG 3 cut(s) 111, 149, 963
HpySE526I ACGT 4 cut(s) 14, 132, 186, 478
Hsp92I GRCGYC 1 cut(s) 14
Hsp92II CATG 3 cut(s) 230, 629, 662
HspAI GCGC 1 cut(s) 279
Kzo9I GATC 2 cut(s) 466, 895
LmnI GCTCC 1 cut(s) 397
Lsp1109I GCAGC 5 cut(s) 38, 223, 389, 679, 877
LweI GCATC 2 cut(s) 353, 364
MaeI CTAG 2 cut(s) 120, 912
MaeII ACGT 4 cut(s) 14, 132, 186, 478
MaeIII GTNAC 2 cut(s) 10, 504
MalI GATC 2 cut(s) 468, 897
MboI GATC 2 cut(s) 466, 895
MboII GAAGA 4 cut(s) 9, 34, 108, 1017
MflI RGATCY 1 cut(s) 895
MhlI GDGCHC 1 cut(s) 983
MlsI TGGCCA 1 cut(s) 843
MluCI AATT 7 cut(s) 272, 295, 591, 749, 770, 853, 884
MluNI TGGCCA 1 cut(s) 843
MlyI GAGTC 3 cut(s) 78, 93, 148
MnlI CCTC 8 cut(s) 86, 106, 152, 401, 488, 525, 664, 710
Mox20I TGGCCA 1 cut(s) 843
MscI TGGCCA 1 cut(s) 843
MseI TTAA 3 cut(s) 294, 299, 756
MslI CAYNNNNRTG 3 cut(s) 231, 349, 385
Msp20I TGGCCA 1 cut(s) 843
MspI CCGG 1 cut(s) 525
MspR9I CCNGG 2 cut(s) 526, 617
MvaI CCWGG 1 cut(s) 617
MwoI GCNNNNNNNGC 1 cut(s) 35
NciI CCSGG 1 cut(s) 526
NcoI CCATGG 2 cut(s) 226, 658
NdeII GATC 2 cut(s) 466, 895
NlaIII CATG 3 cut(s) 230, 629, 662
NlaIV GGNNCC 2 cut(s) 117, 457
NmuCI GTSAC 1 cut(s) 10
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PfeI GAWTC 5 cut(s) 52, 83, 650, 833, 875
PkrI GCNGC 5 cut(s) 28, 238, 379, 669, 892
PleI GAGTC 3 cut(s) 77, 93, 148
PpsI GAGTC 3 cut(s) 77, 93, 148
Ppu21I YACGTR 1 cut(s) 479
Psp6I CCWGG 1 cut(s) 615
PspGI CCWGG 1 cut(s) 615
PspN4I GGNNCC 2 cut(s) 117, 457
PspPI GGNCC 2 cut(s) 247, 455
PstI CTGCAG 1 cut(s) 895
PsuI RGATCY 1 cut(s) 895
RsaI GTAC 5 cut(s) 65, 542, 777, 826, 868
RsaNI GTAC 5 cut(s) 64, 541, 776, 825, 867
RseI CAYNNNNRTG 3 cut(s) 231, 349, 385
SalI GTCGAC 1 cut(s) 930
SaqAI TTAA 3 cut(s) 294, 299, 756
SatI GCNGC 5 cut(s) 27, 237, 378, 668, 891
Sau3AI GATC 2 cut(s) 466, 895
Sau96I GGNCC 2 cut(s) 247, 455
SchI GAGTC 3 cut(s) 78, 93, 148
ScrFI CCNGG 2 cut(s) 526, 617
SduI GDGCHC 1 cut(s) 983
SfaNI GCATC 2 cut(s) 353, 364
SfcI CTRYAG 1 cut(s) 891
SmiMI CAYNNNNRTG 3 cut(s) 231, 349, 385
SmlI CTYRAG 5 cut(s) 143, 392, 490, 734, 935
SmoI CTYRAG 5 cut(s) 143, 392, 490, 734, 935
Sse9I AATT 7 cut(s) 272, 295, 591, 749, 770, 853, 884
SsiI CCGC 4 cut(s) 47, 90, 553, 801
SspMI CTAG 2 cut(s) 120, 912
StyD4I CCNGG 2 cut(s) 524, 615
StyI CCWWGG 3 cut(s) 198, 226, 658
TaaI ACNGT 5 cut(s) 60, 463, 512, 829, 920
TaiI ACGT 4 cut(s) 17, 135, 189, 481
TaqI TCGA 4 cut(s) 258, 585, 931, 973
TaqII GACCGA 1 cut(s) 1002
TasI AATT 7 cut(s) 272, 295, 591, 749, 770, 853, 884
TatI WGTACW 2 cut(s) 775, 824
TfiI GAWTC 5 cut(s) 52, 83, 650, 833, 875
Tru1I TTAA 3 cut(s) 294, 299, 756
Tru9I TTAA 3 cut(s) 294, 299, 756
TscAI CASTG 2 cut(s) 407, 466
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 5 cut(s) 26, 236, 377, 667, 890
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 4 cut(s) 183, 432, 654, 711
TspRI CASTG 2 cut(s) 407, 466
XapI RAATTY 3 cut(s) 749, 770, 853
XbaI TCTAGA 1 cut(s) 911
XmiI GTMKAC 2 cut(s) 481, 931
XspI CTAG 2 cut(s) 120, 912
ZraI GACGTC 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.