Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0009132

Physical Location & Seq

Genomic Coordinates Forward (+)

34411356 .. 34416503

Gene Structure 5148 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1269900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1593 bp

ATGCAGGCTTGTGGTGGAGCTGCAGTGATGGGTTCTCTCCAACAGCCCACTTGGACCAAAGGAACAATTTTTCCAAACAAGGGGCTTACCGGTAGTGGATTTTCACATCAACTAAAGCTCAATTGTGTGAAGCCCGTTAGATCTTCTTCTTACATTGAAGGAAGTTTGGTTGCTGGAAGGCCGTCCTCTTCTGTCTCTGTGCCTATGCCTGAAATTGGCGGTAATGGAAGCAGTTTTGTAGACAATGGGTTAAGTGAGGCTGACCCTGAGGTGCGTGCTATTATTGATATGGAGAAGCAGCGACAGTTCAAGAGTCTGGAGCTTATTGCCTCAGAAAATTTTACTTATCGGGCAGTGATGGAGGCAGTTGGCTCATGTCTCACGAACAAGTATTCAGAAGGATTACCAGGTAAAAGGTACTACGGTGGAAATGAGCATATTGATGAGCTTGAAACACTTTGCCAAAGGAGGGCTCTTACTGCATTTCACTTAGATGGAATGAAGTGGGGTGTTAATGTTCAACCATTATCTGGTTCTCCTGCTAATTTTGAGGTTTATACCGCAATTCTTAAACCTCATGATCGTCTCATGGGTTTGGACTTGTCTCACGGAGGACATTTGTCTCATGGTTTCATGACTCCTAAAAGACGGGTATCAGGCACATCCATTTATTTTGAGTCCATGTCGTATCGACTCAATGAATCCACAGGTCTGGTTGATTATGACAAGCTTGAGGAAATGGCTGACCGATTTAAACCAAAACTCATTATTGCCGGGGCTAGTGCTTATCCTCGAGATTTTGACTATCCTCGCATGAGGAAGATTGCGGATTCTGTTGGTGCTTTTCTTATGATGGATATGGCTCACATAAGTGGGCTTGTTGCTGCTTCTGTGCTTGCAAATCCCTTTGACTACTGCGATATTGTGACCACAACAACACACAAGTCTTTAAGAGGTCCAAGGGGCGGGATGATCTTCTTCAAAAAAGATACAGTTCTCGGGGTTGATTTAGAAACTGCCATCAACAATGCTGTTTTTCCAGGTTTACAGGGTGGTCCTCATAACCACACTATTGGGGGCCTTGCAGTTTGCTTGAAGTATGCACAGTCCCCAGAATTTAAGGCTTACCAGAACCAGGTGGTATCTAATTGCAGGGCTCTTGCGAGCCGATTAACTGAACGTGGCTACAAACTAGTTTCTGGTGGAAGTGATAACCATCTGGTTCTTGTGGATCTCCGGCCTTTAGGTATTGATGGAGCTCGAGTGGAGAAAATTCTTGACATGGCTTCTATCACCCTCAACAAGAACTCAGTACCTGGTGATAAAAGTGCTGTAGTGCCTGGTGGCATTCGCATTGGATCACCCGCCATGACAACAAGAGGATTCACTGAGAAAGAATTCATAGCTGTTGCAGACTATATTCATGAGGGTGTGCAGATAACAATTGATGCTAAACGCGCAGCCTCAGGATCGAAGCTCCAAGATTTCATGAAGTTTGTAGCTTCCCCCGAATTCTCCTTAAAGGACCGAGTGTCAGATCTGCAGAGAAGAGTTGAAGCGCTTACCACCCAGTTCCCGTTGCCTGGAGTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004372 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0016740 GO:0016741 GO:0016742 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00260 ko00460 ko00630 ko00670 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko01523 map00260 map00460 map00630 map00670 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map01523

Pfam Domains

Protein Families

SHMT

Protein Analysis

531

Amino Acids

57.56

Weight (kDa)

8.5

Isoelectric Point (pI)

36.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SHMT	PF00464	84 - 474	4e-192	Serine hydroxymethyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009132)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G32520 AT4G32520
fragaria_vesca	FvH4_7g07540 FvH4_7g07540 FvH4_7g07540
malus_domestica	MD09G1269900.v1.1 MD10G1009100.v1.1 MD17G1267800.v1.1
prunus_persica	Prupe.3G165800_v2.0.a1 Prupe.8G013000_v2.0.a1 Prupe.8G013000_v2.0.a1 Prupe.8G013000_v2.0.a1
pyrus_communis	pycom09g18110 pycom10g00550
rosa_chinensis	RchiOBHm_Chr7g0222651
rosa_laevigata	RLG00000002041
rosa_multiflora	Rmu_sc0001498.1_g000007
rosa_roxburghii	Rroxscaffold_3G00236570
rosa_rugosa	Rorug07G0211700
rosa_samantha	Rh7AG355300 Rh7BG343700
rosa_wichuraiana	Rw7G030180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	530
AccI	GTMKAC	1 cut(s)	240
AccII	CGCG	1 cut(s)	1458
AciI	CCGC	5 cut(s)	219, 561, 827, 966, 1365
AclWI	GGATC	3 cut(s)	1239, 1366, 1477
AcsI	RAATTY	5 cut(s)	337, 1115, 1272, 1397, 1511
AfaI	GTAC	2 cut(s)	419, 1314
AfeI	AGCGCT	1 cut(s)	1560
AfiI	CCNNNNNNNGG	7 cut(s)	80, 215, 469, 530, 965, 1135, 1583
AgeI	ACCGGT	1 cut(s)	89
AgsI	TTSAA	7 cut(s)	158, 310, 452, 521, 982, 1096, 1556
AhdI	GACNNNNNGTC	1 cut(s)	1531
AhlI	ACTAGT	1 cut(s)	1192
AjnI	CCWGG	6 cut(s)	406, 1039, 1134, 1315, 1339, 1582
AleI	CACNNNNGTG	1 cut(s)	870
AloI	GAACNNNNNNTCC	2 cut(s)	55, 87
AluBI	AGCT	9 cut(s)	20, 118, 322, 448, 730, 1259, 1406, 1477, 1502
AluI	AGCT	9 cut(s)	20, 118, 322, 448, 730, 1259, 1406, 1477, 1502
Alw21I	GWGCWC	1 cut(s)	1261
Alw26I	GTCTC	5 cut(s)	199, 383, 590, 609, 627
AlwI	GGATC	3 cut(s)	1239, 1366, 1477
AlwNI	CAGNNNCTG	1 cut(s)	1316
Ama87I	CYCGRG	3 cut(s)	792, 998, 1260
Aor51HI	AGCGCT	1 cut(s)	1560
AoxI	GGCC	3 cut(s)	179, 1078, 1238
ApeKI	GCWGC	4 cut(s)	20, 298, 884, 1460
ApoI	RAATTY	5 cut(s)	337, 1115, 1272, 1397, 1511
AsiGI	ACCGGT	1 cut(s)	89
Asp700I	GAANNNNTTC	1 cut(s)	1397
AspLEI	GCGC	2 cut(s)	1460, 1561
AspS9I	GGNCC	5 cut(s)	54, 956, 1055, 1078, 1525
AsuC2I	CCSGG	1 cut(s)	775
AsuHPI	GGTGA	3 cut(s)	1285, 1331, 1353
AvaI	CYCGRG	3 cut(s)	792, 998, 1260
AvaII	GGWCC	4 cut(s)	54, 956, 1055, 1525
AxyI	CCTNAGG	2 cut(s)	267, 1465
BaeI	ACNNNNGTAYC	2 cut(s)	409, 442
BanII	GRGCYC	3 cut(s)	475, 1159, 1261
Bbv12I	GWGCWC	1 cut(s)	1261
BbvI	GCAGC	4 cut(s)	7, 310, 871, 1472
BccI	CCATC	7 cut(s)	22, 352, 488, 847, 1028, 1224, 1247
BceAI	ACGGC	1 cut(s)	166
BciT130I	CCWGG	6 cut(s)	408, 1041, 1136, 1317, 1341, 1584
BcnI	CCSGG	1 cut(s)	775
BcoDI	GTCTC	5 cut(s)	199, 383, 590, 609, 627
BcuI	ACTAGT	1 cut(s)	1192
BfaI	CTAG	2 cut(s)	780, 1193
BfmI	CTRYAG	3 cut(s)	21, 1332, 1541
BfoI	RGCGCY	1 cut(s)	1562
BglII	AGATCT	2 cut(s)	140, 1537
BisI	GCNGC	4 cut(s)	21, 299, 885, 1461
BlsI	GCNGC	4 cut(s)	22, 300, 886, 1462
Bme1390I	CCNGG	7 cut(s)	408, 775, 1041, 1136, 1317, 1341, 1584
Bme18I	GGWCC	4 cut(s)	54, 956, 1055, 1525
BmeRI	GACNNNNNGTC	1 cut(s)	1531
BmeT110I	CYCGRG	3 cut(s)	792, 998, 1260
BmgT120I	GGNCC	5 cut(s)	54, 956, 1055, 1078, 1525
BmiI	GGNNCC	1 cut(s)	1079
BmrFI	CCNGG	7 cut(s)	408, 775, 1041, 1136, 1317, 1341, 1584
BmrI	ACTGGG	1 cut(s)	1564
BmsI	GCATC	1 cut(s)	1438
BmuI	ACTGGG	1 cut(s)	1564
BoxI	GACNNNNGTC	1 cut(s)	619
BpmI	CTGGAG	1 cut(s)	338
BpuEI	CTTGAG	1 cut(s)	752
BpuMI	CCSGG	1 cut(s)	775
BsaBI	GATNNNNATC	1 cut(s)	1215
BsaJI	CCNNGG	2 cut(s)	774, 959
BsaWI	WCCGGW	1 cut(s)	89
BsaXI	ACNNNNNCTCC	4 cut(s)	9, 39, 1248, 1278
Bsc4I	CCNNNNNNNGG	7 cut(s)	80, 215, 469, 530, 965, 1135, 1583
Bse118I	RCCGGY	1 cut(s)	89
Bse1I	ACTGG	1 cut(s)	1570
Bse21I	CCTNAGG	2 cut(s)	267, 1465
Bse8I	GATNNNNATC	1 cut(s)	1215
BseBI	CCWGG	6 cut(s)	408, 1041, 1136, 1317, 1341, 1584
BseDI	CCNNGG	2 cut(s)	774, 959
BseGI	GGATG	2 cut(s)	662, 975
BseJI	GATNNNNATC	1 cut(s)	1215
BseLI	CCNNNNNNNGG	7 cut(s)	80, 215, 469, 530, 965, 1135, 1583
BseMII	CTCAG	5 cut(s)	258, 345, 1323, 1380, 1479
BseNI	ACTGG	1 cut(s)	1570
BseXI	GCAGC	4 cut(s)	7, 310, 871, 1472
BsgI	GTGCAG	1 cut(s)	1454
Bsh1236I	CGCG	1 cut(s)	1458
BshFI	GGCC	3 cut(s)	181, 1080, 1240
BshTI	ACCGGT	1 cut(s)	89
BsiHKAI	GWGCWC	1 cut(s)	1261
BsiHKCI	CYCGRG	3 cut(s)	792, 998, 1260
BsiSI	CCGG	3 cut(s)	90, 774, 1237
BslFI	GGGAC	1 cut(s)	1093
BslI	CCNNNNNNNGG	7 cut(s)	80, 215, 469, 530, 965, 1135, 1583
BsmAI	GTCTC	5 cut(s)	199, 383, 590, 609, 627
BsmBI	CGTCTC	1 cut(s)	590
BsmFI	GGGAC	1 cut(s)	1093
BsmI	GAATGC	1 cut(s)	1347
BsnI	GGCC	3 cut(s)	181, 1080, 1240
BsoBI	CYCGRG	3 cut(s)	792, 998, 1260
Bsp1286I	GDGCHC	3 cut(s)	475, 1159, 1261
Bsp143I	GATC	7 cut(s)	140, 580, 972, 1231, 1358, 1469, 1537
BspACI	CCGC	5 cut(s)	219, 561, 827, 966, 1365
BspANI	GGCC	3 cut(s)	181, 1080, 1240
BspCNI	CTCAG	5 cut(s)	259, 344, 1322, 1381, 1478
BspFNI	CGCG	1 cut(s)	1458
BspHI	TCATGA	4 cut(s)	577, 633, 1423, 1488
BspLI	GGNNCC	1 cut(s)	1079
BspMAI	CTGCAG	2 cut(s)	25, 1545
BspPI	GGATC	3 cut(s)	1239, 1366, 1477
BsrFI	RCCGGY	1 cut(s)	89
BsrI	ACTGG	1 cut(s)	1570
BssAI	RCCGGY	1 cut(s)	89
BssECI	CCNNGG	2 cut(s)	774, 959
BssMI	GATC	7 cut(s)	140, 580, 972, 1231, 1358, 1469, 1537
BssT1I	CCWWGG	1 cut(s)	959
Bst2UI	CCWGG	6 cut(s)	408, 1041, 1136, 1317, 1341, 1584
Bst4CI	ACNGT	4 cut(s)	306, 425, 994, 1107
Bst6I	CTCTTC	2 cut(s)	193, 1543
BstC8I	GCNNGC	4 cut(s)	6, 276, 897, 1165
BstDEI	CTNAG	6 cut(s)	267, 331, 490, 1309, 1389, 1465
BstF5I	GGATG	2 cut(s)	662, 975
BstFNI	CGCG	1 cut(s)	1458
BstH2I	RGCGCY	1 cut(s)	1562
BstHHI	GCGC	2 cut(s)	1460, 1561
BstKTI	GATC	7 cut(s)	143, 583, 975, 1234, 1361, 1472, 1540
BstMAI	GTCTC	5 cut(s)	199, 383, 590, 609, 627
BstMBI	GATC	7 cut(s)	140, 580, 972, 1231, 1358, 1469, 1537
BstMWI	GCNNNNNNNGC	2 cut(s)	479, 1457
BstNI	CCWGG	6 cut(s)	408, 1041, 1136, 1317, 1341, 1584
BstPAI	GACNNNNGTC	1 cut(s)	619
BstSCI	CCNGG	7 cut(s)	406, 773, 1039, 1134, 1315, 1339, 1582
BstSFI	CTRYAG	3 cut(s)	21, 1332, 1541
BstUI	CGCG	1 cut(s)	1458
BstV1I	GCAGC	4 cut(s)	7, 310, 871, 1472
BstX2I	RGATCY	3 cut(s)	140, 1231, 1537
BstXI	CCANNNNNNTGG	2 cut(s)	712, 1073
BstYI	RGATCY	3 cut(s)	140, 1231, 1537
Bsu36I	CCTNAGG	2 cut(s)	267, 1465
BsuRI	GGCC	3 cut(s)	181, 1080, 1240
BtsCI	GGATG	2 cut(s)	662, 975
BtsI	GCAGTG	2 cut(s)	30, 360
BtsIMutI	CAGTG	3 cut(s)	30, 360, 1386
Cac8I	GCNNGC	4 cut(s)	6, 276, 897, 1165
CaiI	CAGNNNCTG	1 cut(s)	1316
CciI	TCATGA	4 cut(s)	577, 633, 1423, 1488
CfoI	GCGC	2 cut(s)	1460, 1561
Cfr10I	RCCGGY	1 cut(s)	89
Cfr13I	GGNCC	5 cut(s)	54, 956, 1055, 1078, 1525
CsiI	ACCWGGT	3 cut(s)	406, 1134, 1315
Csp6I	GTAC	2 cut(s)	418, 1313
CspAI	ACCGGT	1 cut(s)	89
CviQI	GTAC	2 cut(s)	418, 1313
DdeI	CTNAG	6 cut(s)	267, 331, 490, 1309, 1389, 1465
DpnI	GATC	7 cut(s)	142, 582, 974, 1233, 1360, 1471, 1539
DpnII	GATC	7 cut(s)	140, 580, 972, 1231, 1358, 1469, 1537
DraI	TTTAAA	1 cut(s)	754
DriI	GACNNNNNGTC	1 cut(s)	1531
Eam1104I	CTCTTC	2 cut(s)	193, 1543
Eam1105I	GACNNNNNGTC	1 cut(s)	1531
EarI	CTCTTC	2 cut(s)	193, 1543
Ecl136II	GAGCTC	1 cut(s)	1259
Eco130I	CCWWGG	1 cut(s)	959
Eco24I	GRGCYC	3 cut(s)	475, 1159, 1261
Eco47I	GGWCC	4 cut(s)	54, 956, 1055, 1525
Eco47III	AGCGCT	1 cut(s)	1560
Eco53kI	GAGCTC	1 cut(s)	1259
Eco81I	CCTNAGG	2 cut(s)	267, 1465
Eco88I	CYCGRG	3 cut(s)	792, 998, 1260
EcoICRI	GAGCTC	1 cut(s)	1259
EcoO109I	RGGNCCY	1 cut(s)	1078
EcoRI	GAATTC	2 cut(s)	1397, 1511
EcoRII	CCWGG	6 cut(s)	406, 1039, 1134, 1315, 1339, 1582
EcoT14I	CCWWGG	1 cut(s)	959
EcoT38I	GRGCYC	3 cut(s)	475, 1159, 1261
ErhI	CCWWGG	1 cut(s)	959
Esp3I	CGTCTC	1 cut(s)	590
FaqI	GGGAC	1 cut(s)	1093
FauI	CCCGC	2 cut(s)	959, 1372
FblI	GTMKAC	1 cut(s)	240
Fnu4HI	GCNGC	4 cut(s)	21, 299, 885, 1461
FokI	GGATG	2 cut(s)	649, 982
FriOI	GRGCYC	3 cut(s)	475, 1159, 1261
Fsp4HI	GCNGC	4 cut(s)	21, 299, 885, 1461
FspBI	CTAG	2 cut(s)	780, 1193
GlaI	GCGC	2 cut(s)	1459, 1560
GluI	GCNGC	4 cut(s)	21, 299, 885, 1461
GsuI	CTGGAG	1 cut(s)	338
HaeII	RGCGCY	1 cut(s)	1562
HaeIII	GGCC	3 cut(s)	181, 1080, 1240
HapII	CCGG	3 cut(s)	90, 774, 1237
HhaI	GCGC	2 cut(s)	1460, 1561
Hin6I	GCGC	2 cut(s)	1458, 1559
HinP1I	GCGC	2 cut(s)	1458, 1559
HindIII	AAGCTT	1 cut(s)	728
HinfI	GANTC	7 cut(s)	313, 637, 677, 693, 701, 830, 1383
HpaII	CCGG	3 cut(s)	90, 774, 1237
HphI	GGTGA	3 cut(s)	1285, 1331, 1353
Hpy166II	GTNNAC	2 cut(s)	241, 1046
Hpy188I	TCNGA	3 cut(s)	334, 397, 1537
Hpy8I	GTNNAC	2 cut(s)	241, 1046
HpyAV	CCTTC	3 cut(s)	152, 171, 392
HpyCH4III	ACNGT	4 cut(s)	306, 425, 994, 1107
HpyCH4IV	ACGT	1 cut(s)	1180
HpyF10VI	GCNNNNNNNGC	2 cut(s)	479, 1457
HpyF3I	CTNAG	6 cut(s)	267, 331, 490, 1309, 1389, 1465
HpySE526I	ACGT	1 cut(s)	1180
HspAI	GCGC	2 cut(s)	1458, 1559
Kzo9I	GATC	7 cut(s)	140, 580, 972, 1231, 1358, 1469, 1537
LmnI	GCTCC	4 cut(s)	17, 319, 1256, 1482
Lsp1109I	GCAGC	4 cut(s)	7, 310, 871, 1472
LweI	GCATC	1 cut(s)	1438
MabI	ACCWGGT	3 cut(s)	406, 1134, 1315
MaeI	CTAG	2 cut(s)	780, 1193
MaeII	ACGT	1 cut(s)	1180
MaeIII	GTNAC	1 cut(s)	925
MalI	GATC	7 cut(s)	142, 582, 974, 1233, 1360, 1471, 1539
MboI	GATC	7 cut(s)	140, 580, 972, 1231, 1358, 1469, 1537
MboII	GAAGA	7 cut(s)	135, 138, 180, 832, 967, 970, 1560
MfeI	CAATTG	2 cut(s)	121, 1443
MflI	RGATCY	3 cut(s)	140, 1231, 1537
MhlI	GDGCHC	3 cut(s)	475, 1159, 1261
MlyI	GAGTC	4 cut(s)	322, 631, 686, 687
MmeI	TCCRAC	1 cut(s)	64
MroXI	GAANNNNTTC	1 cut(s)	1397
MseI	TTAA	8 cut(s)	251, 513, 570, 753, 950, 1119, 1172, 1520
MslI	CAYNNNNRTG	4 cut(s)	441, 492, 870, 1428
MspI	CCGG	3 cut(s)	90, 774, 1237
MspR9I	CCNGG	7 cut(s)	408, 775, 1041, 1136, 1317, 1341, 1584
MunI	CAATTG	2 cut(s)	121, 1443
Mva1269I	GAATGC	1 cut(s)	1347
MvaI	CCWGG	6 cut(s)	408, 1041, 1136, 1317, 1341, 1584
MvnI	CGCG	1 cut(s)	1458
MwoI	GCNNNNNNNGC	2 cut(s)	479, 1457
NciI	CCSGG	1 cut(s)	775
NdeII	GATC	7 cut(s)	140, 580, 972, 1231, 1358, 1469, 1537
NlaIV	GGNNCC	1 cut(s)	1079
NmuCI	GTSAC	1 cut(s)	925
OliI	CACNNNNGTG	1 cut(s)	870
PaeR7I	CTCGAG	2 cut(s)	792, 1260
PagI	TCATGA	4 cut(s)	577, 633, 1423, 1488
PctI	GAATGC	1 cut(s)	1347
PdmI	GAANNNNTTC	1 cut(s)	1397
PfeI	GAWTC	3 cut(s)	701, 830, 1383
PflMI	CCANNNNNTGG	1 cut(s)	530
PinAI	ACCGGT	1 cut(s)	89
PkrI	GCNGC	4 cut(s)	22, 300, 886, 1462
PleI	GAGTC	4 cut(s)	321, 631, 685, 687
PpsI	GAGTC	4 cut(s)	321, 631, 685, 687
PshAI	GACNNNNGTC	1 cut(s)	619
Psp124BI	GAGCTC	1 cut(s)	1261
Psp6I	CCWGG	6 cut(s)	406, 1039, 1134, 1315, 1339, 1582
PspGI	CCWGG	6 cut(s)	406, 1039, 1134, 1315, 1339, 1582
PspN4I	GGNNCC	1 cut(s)	1079
PspPI	GGNCC	5 cut(s)	54, 956, 1055, 1078, 1525
PspXI	VCTCGAGB	1 cut(s)	1260
PstI	CTGCAG	2 cut(s)	25, 1545
PstNI	CAGNNNCTG	1 cut(s)	1316
PsuI	RGATCY	3 cut(s)	140, 1231, 1537
RsaI	GTAC	2 cut(s)	419, 1314
RsaNI	GTAC	2 cut(s)	418, 1313
RseI	CAYNNNNRTG	4 cut(s)	441, 492, 870, 1428
SacI	GAGCTC	1 cut(s)	1261
SaqAI	TTAA	8 cut(s)	251, 513, 570, 753, 950, 1119, 1172, 1520
SatI	GCNGC	4 cut(s)	21, 299, 885, 1461
Sau3AI	GATC	7 cut(s)	140, 580, 972, 1231, 1358, 1469, 1537
Sau96I	GGNCC	5 cut(s)	54, 956, 1055, 1078, 1525
SchI	GAGTC	4 cut(s)	322, 631, 686, 687
ScrFI	CCNGG	7 cut(s)	408, 775, 1041, 1136, 1317, 1341, 1584
SduI	GDGCHC	3 cut(s)	475, 1159, 1261
SexAI	ACCWGGT	3 cut(s)	406, 1134, 1315
SfaNI	GCATC	1 cut(s)	1438
SfcI	CTRYAG	3 cut(s)	21, 1332, 1541
Sfr274I	CTCGAG	2 cut(s)	792, 1260
SinI	GGWCC	4 cut(s)	54, 956, 1055, 1525
SlaI	CTCGAG	2 cut(s)	792, 1260
SmiMI	CAYNNNNRTG	4 cut(s)	441, 492, 870, 1428
SmlI	CTYRAG	3 cut(s)	731, 792, 1260
SmoI	CTYRAG	3 cut(s)	731, 792, 1260
SpeI	ACTAGT	1 cut(s)	1192
SsiI	CCGC	5 cut(s)	219, 561, 827, 966, 1365
SspMI	CTAG	2 cut(s)	780, 1193
SstI	GAGCTC	1 cut(s)	1261
StyD4I	CCNGG	7 cut(s)	406, 773, 1039, 1134, 1315, 1339, 1582
StyI	CCWWGG	1 cut(s)	959
TaaI	ACNGT	4 cut(s)	306, 425, 994, 1107
TaiI	ACGT	1 cut(s)	1183
TaqI	TCGA	4 cut(s)	691, 793, 1261, 1472
TaqII	GACCGA	2 cut(s)	762, 1542
TfiI	GAWTC	3 cut(s)	701, 830, 1383
Tru1I	TTAA	8 cut(s)	251, 513, 570, 753, 950, 1119, 1172, 1520
Tru9I	TTAA	8 cut(s)	251, 513, 570, 753, 950, 1119, 1172, 1520
TscAI	CASTG	3 cut(s)	30, 360, 1393
TseFI	GTSAC	1 cut(s)	925
TseI	GCWGC	4 cut(s)	20, 298, 884, 1460
Tsp45I	GTSAC	1 cut(s)	925
TspDTI	ATGAA	7 cut(s)	515, 622, 714, 1390, 1412, 1477, 1505
TspGWI	ACGGA	1 cut(s)	624
TspRI	CASTG	3 cut(s)	30, 360, 1393
Van91I	CCANNNNNTGG	1 cut(s)	530
VpaK11BI	GGWCC	4 cut(s)	54, 956, 1055, 1525
XapI	RAATTY	5 cut(s)	337, 1115, 1272, 1397, 1511
XhoI	CTCGAG	2 cut(s)	792, 1260
XmiI	GTMKAC	1 cut(s)	240
XmnI	GAANNNNTTC	1 cut(s)	1397
XspI	CTAG	2 cut(s)	780, 1193

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.