Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7A

Orthogroup ID

OG0009132

Physical Location & Seq

Genomic Coordinates Reverse (-)

45497777 .. 45501572

Gene Structure 3796 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7AG355300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1575 bp

ATGCAGGCTTGCAGTGGAGCTGCAGTTATGGGTTCTGTTCAACAGCCCACATGGCTTAGAGGAGCAGCTTTTCCAAATAGATTTCCACATCAAGTGAACCTTAATTTTGCAAAGACTGCTAGATCTTATTCTAACATCCAAGGAAGCTTGGTTGCTGGGAGGCCATCCTCTCTGGCTTCAGTGCATGTGCCTGAATTTGGAGATATTGGAAGCAGTTTTGTAGATAATGGCTTAAGTGAGGCTGACCCTGAGGTGCGTGCTATTATTGACAAGGAGAAAGAGCGGCAGTTCAAAAGCCTTGAGCTTATTGCCTCAGAGAATTTCACTTCACGGGCAGTAATGGAGGCAGTTGGCTCATGTCTCACAAACAAGTATTCGGAAGGATTACCTGGGAAAAGGTACTATGGTGGAAATGAGCACATTGATGAGCTTGAAACACTATGCCAAAAAAGGGCCCTGGATGCATTTCACCTAGATGGAAAGAAGTGGGGCGTCAATGTTCAACCATTATCTGGTTCTCCAGCTAATTTCGAGGTTTATACAGCAGTTCTCAATCCTCATGATCGTATCATGGGTTTGGACTTGCCACACGGGGGACATTTGTCTCATGGGTTCATGACTCCTAAAAGACGGGTATCAGGAACATCTATTTATTTTGAGTCCATGCCTTACCGACTTGATGAATCCACAGGTCTGGTTGATTATGACATGCTTGAGAAAACAGCAACCCTATTTCGACCTAAACTCATTATTGTTGGGGCCAGTGCTTATCCTCGAGATTTTGACTATCCTCGCATGAGGAAGATTGCGGATGCTGCTGGTGCTTTTCTTATGATGGATATGGCTCACATAAGTGGGCTTGTTGCTGCTTCTGTGGTTGCCAATCCCTTTGACTACTGTGATATTGTGACAACAACAACCCACAAGTCTCTAAGGGGTCCAAGAGGTGGAATGATATTCTTCAAGAAGGATCCAGTTCTTGGAGTTGATTTAGAAACTGCCATCAACAACGCTGTTTTCCCGGGCTTACAGGGTGGTCCTCATAACCACACTATTGGGGGCCTTGCAGTTTGCTTGAAGCATGCTCAGTCCGCAGAATTTAAGGCTTACCAGAATCAGGTGGTCTCTAATTGCAGGGCTCTTGCAAGCCGATTGGTTGAACTTGAATACACACTGGTTTCTGGTGGAAGCGATAACCACCTGGTTCTTGTCGATCTGCGTCCCTTTGGCATTGATGGTGCTCGGGTGGAGAAAATTCTTGACATGGCGTCTATTACTCTTAACAAGAATTCAGTACCTGGTGATAAAAGTGCTCTAATGCCTGGTGGTATTCGCATTGGAACACCTGCTATGACGACACGAGGATTCACTGAGAAAGACTTCGTAGCAACTGCAGACTACATTCACGAGGGTGTGCGGATAGCGATTGATGCTAAAAGCAAAGCCCCGGGCTCAAAGCTCCAAGAGTTTTTAAAGCATGTTGCAACCCCTGAATTCCCTCTAATTGATCGAGTTTCAGATCTGCGGACAAAAGTCGAAGCTCTTACCACCCAATTCCCGATGCCTGGAGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004372 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0016740 GO:0016741 GO:0016742 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00260 ko00460 ko00630 ko00670 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko01523 map00260 map00460 map00630 map00670 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map01523

Pfam Domains

Protein Families

SHMT

Protein Analysis

524

Amino Acids

56.98

Weight (kDa)

6.98

Isoelectric Point (pI)

31.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SHMT	PF00464	78 - 468	1.9e-191	Serine hydroxymethyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009132)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G32520 AT4G32520
fragaria_vesca	FvH4_7g07540 FvH4_7g07540 FvH4_7g07540
malus_domestica	MD09G1269900.v1.1 MD10G1009100.v1.1 MD17G1267800.v1.1
prunus_persica	Prupe.3G165800_v2.0.a1 Prupe.8G013000_v2.0.a1 Prupe.8G013000_v2.0.a1 Prupe.8G013000_v2.0.a1
pyrus_communis	pycom09g18110 pycom10g00550
rosa_chinensis	RchiOBHm_Chr7g0222651
rosa_laevigata	RLG00000002041
rosa_multiflora	Rmu_sc0001498.1_g000007
rosa_roxburghii	Rroxscaffold_3G00236570
rosa_rugosa	Rorug07G0211700
rosa_samantha	Rh7AG355300 Rh7BG343700
rosa_wichuraiana	Rw7G030180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1354
Acc36I	ACCTGC	1 cut(s)	1354
AccB7I	CCANNNNNTGG	3 cut(s)	512, 947, 980
AccBSI	CCGCTC	1 cut(s)	283
AciI	CCGC	5 cut(s)	283, 809, 1092, 1417, 1525
AclWI	GGATC	2 cut(s)	965, 978
AcsI	RAATTY	6 cut(s)	194, 319, 1097, 1254, 1288, 1493
AcuI	CTGAAG	1 cut(s)	162
AcyI	GRCGYC	2 cut(s)	492, 1268
AfaI	GTAC	2 cut(s)	401, 1296
AfiI	CCNNNNNNNGG	8 cut(s)	197, 451, 512, 593, 947, 980, 1117, 1565
AflII	CTTAAG	1 cut(s)	232
AgsI	TTSAA	8 cut(s)	41, 292, 434, 503, 964, 1078, 1160, 1166
AhdI	GACNNNNNGTC	1 cut(s)	1267
AjnI	CCWGG	6 cut(s)	388, 456, 1200, 1297, 1321, 1564
AleI	CACNNNNGTG	2 cut(s)	852, 1410
AluBI	AGCT	8 cut(s)	20, 68, 147, 304, 430, 524, 1459, 1541
AluI	AGCT	8 cut(s)	20, 68, 147, 304, 430, 524, 1459, 1541
Alw21I	GWGCWC	3 cut(s)	420, 1243, 1315
Alw26I	GTCTC	4 cut(s)	365, 609, 933, 1129
AlwI	GGATC	2 cut(s)	965, 978
AlwNI	CAGNNNCTG	1 cut(s)	1298
Ama87I	CYCGRG	4 cut(s)	774, 1021, 1242, 1447
AoxI	GGCC	4 cut(s)	161, 453, 759, 1060
ApaI	GGGCCC	1 cut(s)	457
ApeKI	GCWGC	4 cut(s)	20, 65, 815, 866
ApoI	RAATTY	6 cut(s)	194, 319, 1097, 1254, 1288, 1493
Asp700I	GAANNNNTTC	1 cut(s)	1379
AspS9I	GGNCC	6 cut(s)	453, 454, 759, 938, 1037, 1060
AsuC2I	CCSGG	4 cut(s)	1022, 1023, 1448, 1449
AsuHPI	GGTGA	2 cut(s)	461, 1313
AvaI	CYCGRG	4 cut(s)	774, 1021, 1242, 1447
AvaII	GGWCC	2 cut(s)	938, 1037
AxyI	CCTNAGG	1 cut(s)	249
BaeGI	GKGCMC	1 cut(s)	457
BaeI	ACNNNNGTAYC	2 cut(s)	391, 424
BamHI	GGATCC	1 cut(s)	970
BanII	GRGCYC	3 cut(s)	457, 1141, 1454
BauI	CACGAG	2 cut(s)	1359, 1406
Bbv12I	GWGCWC	3 cut(s)	420, 1243, 1315
BbvI	GCAGC	4 cut(s)	7, 77, 802, 853
BccI	CCATC	5 cut(s)	172, 470, 829, 1010, 1229
BciT130I	CCWGG	6 cut(s)	390, 458, 1202, 1299, 1323, 1566
BcnI	CCSGG	4 cut(s)	1022, 1023, 1448, 1449
BcoDI	GTCTC	4 cut(s)	365, 609, 933, 1129
BfaI	CTAG	2 cut(s)	120, 473
BfmI	CTRYAG	2 cut(s)	21, 1392
BfrI	CTTAAG	1 cut(s)	232
BfuAI	ACCTGC	1 cut(s)	1354
BglI	GCCNNNNNGGC	1 cut(s)	52
BglII	AGATCT	2 cut(s)	122, 1519
BisI	GCNGC	5 cut(s)	21, 66, 284, 816, 867
BlsI	GCNGC	5 cut(s)	22, 67, 285, 817, 868
Bme18I	GGWCC	2 cut(s)	938, 1037
BmeRI	GACNNNNNGTC	1 cut(s)	1267
BmeT110I	CYCGRG	4 cut(s)	774, 1021, 1242, 1447
BmgT120I	GGNCC	6 cut(s)	453, 454, 759, 938, 1037, 1060
BmiI	GGNNCC	5 cut(s)	455, 760, 939, 972, 1061
BmsI	GCATC	4 cut(s)	451, 802, 1420, 1551
BoxI	GACNNNNGTC	2 cut(s)	601, 1532
BpmI	CTGGAG	1 cut(s)	504
BpuEI	CTTGAG	2 cut(s)	320, 734
BpuMI	CCSGG	4 cut(s)	1022, 1023, 1448, 1449
BsaHI	GRCGYC	2 cut(s)	492, 1268
BsaI	GGTCTC	1 cut(s)	1129
BsaJI	CCNNGG	6 cut(s)	139, 389, 456, 1021, 1446, 1447
BsaXI	ACNNNNNCTCC	2 cut(s)	9, 39
Bsc4I	CCNNNNNNNGG	8 cut(s)	197, 451, 512, 593, 947, 980, 1117, 1565
Bse1I	ACTGG	3 cut(s)	762, 974, 1179
Bse21I	CCTNAGG	1 cut(s)	249
BseBI	CCWGG	6 cut(s)	390, 458, 1202, 1299, 1323, 1566
BseDI	CCNNGG	6 cut(s)	139, 389, 456, 1021, 1446, 1447
BseGI	GGATG	4 cut(s)	135, 164, 466, 817
BseLI	CCNNNNNNNGG	8 cut(s)	197, 451, 512, 593, 947, 980, 1117, 1565
BseMII	CTCAG	4 cut(s)	240, 327, 1100, 1362
BseNI	ACTGG	3 cut(s)	762, 974, 1179
BseRI	GAGGAG	1 cut(s)	75
BseSI	GKGCMC	1 cut(s)	457
BseXI	GCAGC	4 cut(s)	7, 77, 802, 853
BseYI	CCCAGC	1 cut(s)	155
BshFI	GGCC	4 cut(s)	163, 455, 761, 1062
BsiHKAI	GWGCWC	3 cut(s)	420, 1243, 1315
BsiHKCI	CYCGRG	4 cut(s)	774, 1021, 1242, 1447
BsiSI	CCGG	2 cut(s)	1022, 1448
BslFI	GGGAC	2 cut(s)	609, 1206
BslI	CCNNNNNNNGG	8 cut(s)	197, 451, 512, 593, 947, 980, 1117, 1565
BsmAI	GTCTC	4 cut(s)	365, 609, 933, 1129
BsmFI	GGGAC	2 cut(s)	609, 1206
BsnI	GGCC	4 cut(s)	163, 455, 761, 1062
Bso31I	GGTCTC	1 cut(s)	1129
BsoBI	CYCGRG	4 cut(s)	774, 1021, 1242, 1447
Bsp120I	GGGCCC	1 cut(s)	453
Bsp1286I	GDGCHC	6 cut(s)	420, 457, 1141, 1243, 1315, 1454
Bsp143I	GATC	6 cut(s)	122, 562, 970, 1213, 1507, 1519
BspACI	CCGC	5 cut(s)	283, 809, 1092, 1417, 1525
BspANI	GGCC	4 cut(s)	163, 455, 761, 1062
BspCNI	CTCAG	4 cut(s)	241, 326, 1099, 1363
BspHI	TCATGA	2 cut(s)	559, 615
BspLI	GGNNCC	5 cut(s)	455, 760, 939, 972, 1061
BspMAI	CTGCAG	2 cut(s)	25, 1396
BspMI	ACCTGC	1 cut(s)	1354
BspPI	GGATC	2 cut(s)	965, 978
BspTI	CTTAAG	1 cut(s)	232
BspTNI	GGTCTC	1 cut(s)	1129
BsrBI	CCGCTC	1 cut(s)	283
BsrI	ACTGG	3 cut(s)	762, 974, 1179
BssECI	CCNNGG	6 cut(s)	139, 389, 456, 1021, 1446, 1447
BssMI	GATC	6 cut(s)	122, 562, 970, 1213, 1507, 1519
BssNI	GRCGYC	2 cut(s)	492, 1268
BssSI	CACGAG	2 cut(s)	1359, 1406
BssT1I	CCWWGG	1 cut(s)	139
Bst2BI	CACGAG	2 cut(s)	1359, 1406
Bst2UI	CCWGG	6 cut(s)	390, 458, 1202, 1299, 1323, 1566
Bst4CI	ACNGT	1 cut(s)	899
BstACI	GRCGYC	2 cut(s)	492, 1268
BstAFI	CTTAAG	1 cut(s)	232
BstAPI	GCANNNNNTGC	1 cut(s)	116
BstC8I	GCNNGC	5 cut(s)	6, 10, 258, 1083, 1147
BstDEI	CTNAG	6 cut(s)	56, 249, 313, 932, 1086, 1371
BstF5I	GGATG	4 cut(s)	135, 164, 466, 817
BstKTI	GATC	6 cut(s)	125, 565, 973, 1216, 1510, 1522
BstMAI	GTCTC	4 cut(s)	365, 609, 933, 1129
BstMBI	GATC	6 cut(s)	122, 562, 970, 1213, 1507, 1519
BstMWI	GCNNNNNNNGC	7 cut(s)	52, 116, 461, 815, 821, 1091, 1430
BstNI	CCWGG	6 cut(s)	390, 458, 1202, 1299, 1323, 1566
BstNSI	RCATGY	4 cut(s)	188, 712, 1085, 1481
BstPAI	GACNNNNGTC	2 cut(s)	601, 1532
BstSFI	CTRYAG	2 cut(s)	21, 1392
BstSLI	GKGCMC	1 cut(s)	457
BstV1I	GCAGC	4 cut(s)	7, 77, 802, 853
BstX2I	RGATCY	3 cut(s)	122, 970, 1519
BstXI	CCANNNNNNTGG	2 cut(s)	694, 1055
BstYI	RGATCY	3 cut(s)	122, 970, 1519
Bsu36I	CCTNAGG	1 cut(s)	249
BsuRI	GGCC	4 cut(s)	163, 455, 761, 1062
BtsCI	GGATG	4 cut(s)	135, 164, 466, 817
BtsI	GCAGTG	1 cut(s)	19
BtsIMutI	CAGTG	5 cut(s)	19, 186, 769, 1172, 1368
BveI	ACCTGC	1 cut(s)	1354
Cac8I	GCNNGC	5 cut(s)	6, 10, 258, 1083, 1147
CaiI	CAGNNNCTG	1 cut(s)	1298
CciI	TCATGA	2 cut(s)	559, 615
Cfr13I	GGNCC	6 cut(s)	453, 454, 759, 938, 1037, 1060
Cfr9I	CCCGGG	2 cut(s)	1021, 1447
CseI	GACGC	3 cut(s)	481, 1208, 1257
CsiI	ACCWGGT	2 cut(s)	1200, 1297
Csp6I	GTAC	2 cut(s)	400, 1295
CviQI	GTAC	2 cut(s)	400, 1295
DdeI	CTNAG	6 cut(s)	56, 249, 313, 932, 1086, 1371
DpnI	GATC	6 cut(s)	124, 564, 972, 1215, 1509, 1521
DpnII	GATC	6 cut(s)	122, 562, 970, 1213, 1507, 1519
DraI	TTTAAA	1 cut(s)	1473
DriI	GACNNNNNGTC	1 cut(s)	1267
Eam1105I	GACNNNNNGTC	1 cut(s)	1267
Eco130I	CCWWGG	1 cut(s)	139
Eco24I	GRGCYC	3 cut(s)	457, 1141, 1454
Eco31I	GGTCTC	1 cut(s)	1129
Eco47I	GGWCC	2 cut(s)	938, 1037
Eco57I	CTGAAG	1 cut(s)	162
Eco81I	CCTNAGG	1 cut(s)	249
Eco88I	CYCGRG	4 cut(s)	774, 1021, 1242, 1447
EcoO109I	RGGNCCY	3 cut(s)	453, 454, 1060
EcoRI	GAATTC	2 cut(s)	1288, 1493
EcoRII	CCWGG	6 cut(s)	388, 456, 1200, 1297, 1321, 1564
EcoT14I	CCWWGG	1 cut(s)	139
EcoT22I	ATGCAT	1 cut(s)	466
EcoT38I	GRGCYC	3 cut(s)	457, 1141, 1454
ErhI	CCWWGG	1 cut(s)	139
FalI	AAGNNNNNCTT	2 cut(s)	84, 116
FaqI	GGGAC	2 cut(s)	609, 1206
Fnu4HI	GCNGC	5 cut(s)	21, 66, 284, 816, 867
FokI	GGATG	4 cut(s)	122, 151, 473, 824
FriOI	GRGCYC	3 cut(s)	457, 1141, 1454
Fsp4HI	GCNGC	5 cut(s)	21, 66, 284, 816, 867
FspBI	CTAG	2 cut(s)	120, 473
GluI	GCNGC	5 cut(s)	21, 66, 284, 816, 867
GsaI	CCCAGC	1 cut(s)	159
GsuI	CTGGAG	1 cut(s)	504
HaeIII	GGCC	4 cut(s)	163, 455, 761, 1062
HapII	CCGG	2 cut(s)	1022, 1448
HgaI	GACGC	3 cut(s)	481, 1208, 1257
Hin1I	GRCGYC	2 cut(s)	492, 1268
HindIII	AAGCTT	1 cut(s)	145
HinfI	GANTC	5 cut(s)	619, 659, 683, 1114, 1365
HpaII	CCGG	2 cut(s)	1022, 1448
HphI	GGTGA	2 cut(s)	461, 1313
Hpy166II	GTNNAC	1 cut(s)	97
Hpy188I	TCNGA	3 cut(s)	316, 379, 1519
Hpy188III	TCNNGA	8 cut(s)	560, 616, 639, 776, 964, 1259, 1406, 1558
Hpy8I	GTNNAC	1 cut(s)	97
HpyAV	CCTTC	2 cut(s)	374, 961
HpyCH4III	ACNGT	1 cut(s)	899
HpyF10VI	GCNNNNNNNGC	7 cut(s)	52, 116, 461, 815, 821, 1091, 1430
HpyF3I	CTNAG	6 cut(s)	56, 249, 313, 932, 1086, 1371
Hsp92I	GRCGYC	2 cut(s)	492, 1268
Kzo9I	GATC	6 cut(s)	122, 562, 970, 1213, 1507, 1519
LmnI	GCTCC	3 cut(s)	17, 62, 1464
Lsp1109I	GCAGC	4 cut(s)	7, 77, 802, 853
LweI	GCATC	4 cut(s)	451, 802, 1420, 1551
MabI	ACCWGGT	2 cut(s)	1200, 1297
MaeI	CTAG	2 cut(s)	120, 473
MaeIII	GTNAC	1 cut(s)	907
MalI	GATC	6 cut(s)	124, 564, 972, 1215, 1509, 1521
MbiI	CCGCTC	1 cut(s)	283
MboI	GATC	6 cut(s)	122, 562, 970, 1213, 1507, 1519
MboII	GAAGA	2 cut(s)	814, 952
MflI	RGATCY	3 cut(s)	122, 970, 1519
MhlI	GDGCHC	6 cut(s)	420, 457, 1141, 1243, 1315, 1454
MlyI	GAGTC	2 cut(s)	613, 668
Mph1103I	ATGCAT	1 cut(s)	466
MroXI	GAANNNNTTC	1 cut(s)	1379
MseI	TTAA	5 cut(s)	102, 233, 1101, 1281, 1472
MslI	CAYNNNNRTG	4 cut(s)	423, 474, 852, 1410
MspCI	CTTAAG	1 cut(s)	232
MspI	CCGG	2 cut(s)	1022, 1448
MvaI	CCWGG	6 cut(s)	390, 458, 1202, 1299, 1323, 1566
MwoI	GCNNNNNNNGC	7 cut(s)	52, 116, 461, 815, 821, 1091, 1430
NciI	CCSGG	4 cut(s)	1022, 1023, 1448, 1449
NdeII	GATC	6 cut(s)	122, 562, 970, 1213, 1507, 1519
NlaIV	GGNNCC	5 cut(s)	455, 760, 939, 972, 1061
NmuCI	GTSAC	1 cut(s)	907
NsiI	ATGCAT	1 cut(s)	466
NspI	RCATGY	4 cut(s)	188, 712, 1085, 1481
OliI	CACNNNNGTG	2 cut(s)	852, 1410
PaeI	GCATGC	1 cut(s)	1085
PaeR7I	CTCGAG	1 cut(s)	774
PagI	TCATGA	2 cut(s)	559, 615
PaqCI	CACCTGC	1 cut(s)	1354
PdmI	GAANNNNTTC	1 cut(s)	1379
PfeI	GAWTC	3 cut(s)	683, 1114, 1365
PflMI	CCANNNNNTGG	3 cut(s)	512, 947, 980
PkrI	GCNGC	5 cut(s)	22, 67, 285, 817, 868
PleI	GAGTC	2 cut(s)	613, 667
PpsI	GAGTC	2 cut(s)	613, 667
PshAI	GACNNNNGTC	2 cut(s)	601, 1532
Psp6I	CCWGG	6 cut(s)	388, 456, 1200, 1297, 1321, 1564
PspFI	CCCAGC	1 cut(s)	155
PspGI	CCWGG	6 cut(s)	388, 456, 1200, 1297, 1321, 1564
PspN4I	GGNNCC	5 cut(s)	455, 760, 939, 972, 1061
PspOMI	GGGCCC	1 cut(s)	453
PspPI	GGNCC	6 cut(s)	453, 454, 759, 938, 1037, 1060
PstI	CTGCAG	2 cut(s)	25, 1396
PstNI	CAGNNNCTG	1 cut(s)	1298
PsuI	RGATCY	3 cut(s)	122, 970, 1519
RsaI	GTAC	2 cut(s)	401, 1296
RsaNI	GTAC	2 cut(s)	400, 1295
RseI	CAYNNNNRTG	4 cut(s)	423, 474, 852, 1410
SaqAI	TTAA	5 cut(s)	102, 233, 1101, 1281, 1472
SatI	GCNGC	5 cut(s)	21, 66, 284, 816, 867
Sau3AI	GATC	6 cut(s)	122, 562, 970, 1213, 1507, 1519
Sau96I	GGNCC	6 cut(s)	453, 454, 759, 938, 1037, 1060
SchI	GAGTC	2 cut(s)	613, 668
SduI	GDGCHC	6 cut(s)	420, 457, 1141, 1243, 1315, 1454
SexAI	ACCWGGT	2 cut(s)	1200, 1297
SfaNI	GCATC	4 cut(s)	451, 802, 1420, 1551
SfcI	CTRYAG	2 cut(s)	21, 1392
Sfr274I	CTCGAG	1 cut(s)	774
SinI	GGWCC	2 cut(s)	938, 1037
SlaI	CTCGAG	1 cut(s)	774
SmaI	CCCGGG	2 cut(s)	1023, 1449
SmiMI	CAYNNNNRTG	4 cut(s)	423, 474, 852, 1410
SmlI	CTYRAG	4 cut(s)	232, 299, 713, 774
SmoI	CTYRAG	4 cut(s)	232, 299, 713, 774
SphI	GCATGC	1 cut(s)	1085
SsiI	CCGC	5 cut(s)	283, 809, 1092, 1417, 1525
SspMI	CTAG	2 cut(s)	120, 473
StyI	CCWWGG	1 cut(s)	139
TaaI	ACNGT	1 cut(s)	899
TaqI	TCGA	6 cut(s)	531, 736, 775, 1212, 1510, 1536
TauI	GCSGC	1 cut(s)	286
TfiI	GAWTC	3 cut(s)	683, 1114, 1365
Tru1I	TTAA	5 cut(s)	102, 233, 1101, 1281, 1472
Tru9I	TTAA	5 cut(s)	102, 233, 1101, 1281, 1472
TscAI	CASTG	5 cut(s)	19, 186, 769, 1179, 1375
TseFI	GTSAC	1 cut(s)	907
TseI	GCWGC	4 cut(s)	20, 65, 815, 866
Tsp45I	GTSAC	1 cut(s)	907
TspDTI	ATGAA	2 cut(s)	604, 696
TspMI	CCCGGG	2 cut(s)	1021, 1447
TspRI	CASTG	5 cut(s)	19, 186, 769, 1179, 1375
Van91I	CCANNNNNTGG	3 cut(s)	512, 947, 980
Vha464I	CTTAAG	1 cut(s)	232
VpaK11BI	GGWCC	2 cut(s)	938, 1037
XapI	RAATTY	6 cut(s)	194, 319, 1097, 1254, 1288, 1493
XceI	RCATGY	4 cut(s)	188, 712, 1085, 1481
XhoI	CTCGAG	1 cut(s)	774
XmaI	CCCGGG	2 cut(s)	1021, 1447
XmnI	GAANNNNTTC	1 cut(s)	1379
XspI	CTAG	2 cut(s)	120, 473
Zsp2I	ATGCAT	1 cut(s)	466

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.