Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0009132

Physical Location & Seq

Genomic Coordinates Forward (+)

24623340 .. 24628455

Gene Structure 5116 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000002041

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1728 bp

ATGCAGGCTTGCAGTGGAGCTGCAGTTATGGGTTCTGTTCAACAGCCCACATGGCTTAGAGGAGCAGCTTTTCCAAATAGACGACTTAGCATTAGTGGATTTCCACATCAAGTGAACCTTAATTTTGCAAAGACTGCTAGATCTTATTCTAACATCCAAGGAAGCGTGGTTGCTGGGAGGCCATCCTCTCCGGCTTCAGTGCATGTGCCTGAATTTGGAGATATTGGAAGCAGTTTTGTAGATAATGGCTTAAGTGAGGCTGACCCTGAGGTGCGTGCTATTATTGACAAGGAGAAAGAGCGGCAGTTCAAAAGCCTTGAGCTTATTGCCTCAGAGAATTTCACTTCACGGGCAGTAATGGAGGCAGTTGGCTCATGTCTCACAAACAAGTATTCGGAAGGATTACCTGGGAAAAGGTACTATGGTGGAAATGAGCACATTGATGAGCTTGAAACACTATGCCAAAAAAGGGCCCTGGATGCATTTCACCTAGATGGAAAGAAGTGGGGCGTCAATGTTCAACCATTATCTGGTTCTCCAGCTAATTTCGAGGTTTATACAGCAGTTCTCAATCCTCATGATCGTATCATGGGTTTGGACTTGCCACACGGGGGACATTTGTCTCATGGGTTCATGACTCCTAAAAGACGGGTATCAGGAACATCTATTTATTTTGAGTCCATGCCTTACCGACTTGATGAATCCACAGGTCTGGTTGATTATGACATGCTTGAGAAAACAGCAACCCTGTTTCGACCTAAACTCATTATTGTTGGGGCCAGTGCTTATCCTCGAGATTTTGACTATCCTCGCATGAGGAAGATTGCGGATGCTGCTGGTGCTTTTCTTATGATGGATATGGCTCACATAAGTGGTCTTGTTGCTGCTTCTGTGGTTGCCAATCCCTTTGACTACTGTGATATTGTGACAACAACAACCCACAAGTCTCTAAGGGGTCCAAGAGGTGGAATGATATTCTTCAAGAAGGATCCAGTTCTTGGAGTTGATTTAGAAACTGCCATCAACAATGCTGTTTTTCCGGGCTTACAGGGTGGTCCTCATAACCACACTATTGGGGGCCTTGCAGTTTGCTTGAAGCATGCTCAGTCCGCAGAATTTAAGGCTTACCAAAATCAGGTGGTCTCTAATTGCAGGGCTCTTGCAAGCCGATTGGTTGAACTTGAATACACACTGGTTTCTGGTGGAAGCGATAACCACCTGGTTCTTGTCGATCTGCGTCCCTTTGGCATTGATGGTGCTCGGGTGGAGAAAATTCTTGATATGGCTTCTATTACTCTTAACAAGAATTCAGTACCCGGTGATAAAAGTGCTCTAATGCCTGGTGGTATTCGCATTGGAACACCTGCTATGACGACACGAGGATTCACTGAGAAAGACTTCATAGCAACTGCAGACTACATTCACGAGGGTGTGCAGATAGCGATTGATGCTAAAAGCAAAGCCCCAGGCTCAAAGCTCCAAGAGTTTTTAAAGCATGTTGCAACCCCTGAATTCCCTCTAATTGATCGAGTTTCAGATCTGCGGACAAAAGTCGAAGCTCTTACCACCCAATTCCCGATGCCTGGAAGTGAAAGACTGCTTGTATGCACTGTTTGGTGTGCACAAAATCTTCTATTTTGTATGAAGAAGATGAGGCAAAGGTCCATTGTTGGACCAATAATATTCACATGGTTATGGGCCTCATACTTTGTGCGATGTTCCTACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004372 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0016740 GO:0016741 GO:0016742 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00260 ko00460 ko00630 ko00670 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko01523 map00260 map00460 map00630 map00670 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map01523

Pfam Domains

Protein Families

SHMT

Protein Analysis

576

Amino Acids

62.98

Weight (kDa)

8.4

Isoelectric Point (pI)

34.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SHMT	PF00464	84 - 474	4e-191	Serine hydroxymethyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009132)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G32520 AT4G32520
fragaria_vesca	FvH4_7g07540 FvH4_7g07540 FvH4_7g07540
malus_domestica	MD09G1269900.v1.1 MD10G1009100.v1.1 MD17G1267800.v1.1
prunus_persica	Prupe.3G165800_v2.0.a1 Prupe.8G013000_v2.0.a1 Prupe.8G013000_v2.0.a1 Prupe.8G013000_v2.0.a1
pyrus_communis	pycom09g18110 pycom10g00550
rosa_chinensis	RchiOBHm_Chr7g0222651
rosa_laevigata	RLG00000002041
rosa_multiflora	Rmu_sc0001498.1_g000007
rosa_roxburghii	Rroxscaffold_3G00236570
rosa_rugosa	Rorug07G0211700
rosa_samantha	Rh7AG355300 Rh7BG343700
rosa_wichuraiana	Rw7G030180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1372
Acc36I	ACCTGC	1 cut(s)	1372
AccB7I	CCANNNNNTGG	3 cut(s)	530, 965, 998
AccBSI	CCGCTC	1 cut(s)	301
AciI	CCGC	4 cut(s)	301, 827, 1110, 1543
AclWI	GGATC	2 cut(s)	983, 996
AcsI	RAATTY	6 cut(s)	212, 337, 1115, 1272, 1306, 1511
AcuI	CTGAAG	1 cut(s)	180
AcyI	GRCGYC	1 cut(s)	510
AfaI	GTAC	2 cut(s)	419, 1314
AfiI	CCNNNNNNNGG	8 cut(s)	215, 469, 530, 611, 965, 998, 1135, 1583
AflII	CTTAAG	1 cut(s)	250
AgsI	TTSAA	8 cut(s)	41, 310, 452, 521, 982, 1096, 1178, 1184
AjnI	CCWGG	6 cut(s)	406, 474, 1218, 1339, 1465, 1582
AleI	CACNNNNGTG	2 cut(s)	870, 1428
AluBI	AGCT	7 cut(s)	20, 68, 322, 448, 542, 1477, 1559
AluI	AGCT	7 cut(s)	20, 68, 322, 448, 542, 1477, 1559
Alw21I	GWGCWC	4 cut(s)	438, 1261, 1333, 1624
Alw26I	GTCTC	4 cut(s)	383, 627, 951, 1147
Alw44I	GTGCAC	1 cut(s)	1620
AlwI	GGATC	2 cut(s)	983, 996
Ama87I	CYCGRG	2 cut(s)	792, 1260
AoxI	GGCC	5 cut(s)	179, 471, 777, 1078, 1698
ApaI	GGGCCC	1 cut(s)	475
ApaLI	GTGCAC	1 cut(s)	1620
ApeKI	GCWGC	4 cut(s)	20, 65, 833, 884
ApoI	RAATTY	6 cut(s)	212, 337, 1115, 1272, 1306, 1511
Asp700I	GAANNNNTTC	1 cut(s)	1397
AspS9I	GGNCC	9 cut(s)	471, 472, 777, 956, 1055, 1078, 1662, 1673, 1698
AsuC2I	CCSGG	2 cut(s)	1041, 1317
AsuHPI	GGTGA	2 cut(s)	479, 1331
AvaI	CYCGRG	2 cut(s)	792, 1260
AvaII	GGWCC	4 cut(s)	956, 1055, 1662, 1673
AxyI	CCTNAGG	1 cut(s)	267
BaeGI	GKGCMC	2 cut(s)	475, 1624
BaeI	ACNNNNGTAYC	2 cut(s)	409, 442
BamHI	GGATCC	1 cut(s)	988
BanII	GRGCYC	2 cut(s)	475, 1159
BauI	CACGAG	2 cut(s)	1377, 1424
Bbv12I	GWGCWC	4 cut(s)	438, 1261, 1333, 1624
BbvI	GCAGC	4 cut(s)	7, 77, 820, 871
BccI	CCATC	5 cut(s)	190, 488, 847, 1028, 1247
BciT130I	CCWGG	6 cut(s)	408, 476, 1220, 1341, 1467, 1584
BcnI	CCSGG	2 cut(s)	1041, 1317
BcoDI	GTCTC	4 cut(s)	383, 627, 951, 1147
BfaI	CTAG	2 cut(s)	138, 491
BfmI	CTRYAG	2 cut(s)	21, 1410
BfrI	CTTAAG	1 cut(s)	250
BfuAI	ACCTGC	1 cut(s)	1372
BglI	GCCNNNNNGGC	1 cut(s)	52
BglII	AGATCT	2 cut(s)	140, 1537
BisI	GCNGC	5 cut(s)	21, 66, 302, 834, 885
BlsI	GCNGC	5 cut(s)	22, 67, 303, 835, 886
Bme1390I	CCNGG	8 cut(s)	408, 476, 1041, 1220, 1317, 1341, 1467, 1584
Bme18I	GGWCC	4 cut(s)	956, 1055, 1662, 1673
BmeT110I	CYCGRG	2 cut(s)	792, 1260
BmgT120I	GGNCC	9 cut(s)	471, 472, 777, 956, 1055, 1078, 1662, 1673, 1698
BmiI	GGNNCC	5 cut(s)	473, 778, 957, 990, 1079
BmrFI	CCNGG	8 cut(s)	408, 476, 1041, 1220, 1317, 1341, 1467, 1584
BmsI	GCATC	4 cut(s)	469, 820, 1438, 1569
BoxI	GACNNNNGTC	2 cut(s)	619, 1550
BpmI	CTGGAG	1 cut(s)	522
BpuEI	CTTGAG	2 cut(s)	338, 752
BpuMI	CCSGG	2 cut(s)	1041, 1317
BsaHI	GRCGYC	1 cut(s)	510
BsaI	GGTCTC	1 cut(s)	1147
BsaJI	CCNNGG	4 cut(s)	157, 407, 474, 1465
BsaXI	ACNNNNNCTCC	2 cut(s)	9, 39
Bsc4I	CCNNNNNNNGG	8 cut(s)	215, 469, 530, 611, 965, 998, 1135, 1583
Bse1I	ACTGG	3 cut(s)	780, 992, 1197
Bse21I	CCTNAGG	1 cut(s)	267
BseBI	CCWGG	6 cut(s)	408, 476, 1220, 1341, 1467, 1584
BseDI	CCNNGG	4 cut(s)	157, 407, 474, 1465
BseGI	GGATG	4 cut(s)	153, 182, 484, 835
BseLI	CCNNNNNNNGG	8 cut(s)	215, 469, 530, 611, 965, 998, 1135, 1583
BseMII	CTCAG	4 cut(s)	258, 345, 1118, 1380
BseNI	ACTGG	3 cut(s)	780, 992, 1197
BseRI	GAGGAG	1 cut(s)	75
BseSI	GKGCMC	2 cut(s)	475, 1624
BseXI	GCAGC	4 cut(s)	7, 77, 820, 871
BseYI	CCCAGC	1 cut(s)	173
BsgI	GTGCAG	1 cut(s)	1454
BshFI	GGCC	5 cut(s)	181, 473, 779, 1080, 1700
BsiHKAI	GWGCWC	4 cut(s)	438, 1261, 1333, 1624
BsiHKCI	CYCGRG	2 cut(s)	792, 1260
BsiSI	CCGG	3 cut(s)	191, 1040, 1317
BslFI	GGGAC	2 cut(s)	627, 1224
BslI	CCNNNNNNNGG	8 cut(s)	215, 469, 530, 611, 965, 998, 1135, 1583
BsmAI	GTCTC	4 cut(s)	383, 627, 951, 1147
BsmFI	GGGAC	2 cut(s)	627, 1224
BsnI	GGCC	5 cut(s)	181, 473, 779, 1080, 1700
Bso31I	GGTCTC	1 cut(s)	1147
BsoBI	CYCGRG	2 cut(s)	792, 1260
Bsp120I	GGGCCC	1 cut(s)	471
Bsp1286I	GDGCHC	6 cut(s)	438, 475, 1159, 1261, 1333, 1624
Bsp143I	GATC	6 cut(s)	140, 580, 988, 1231, 1525, 1537
BspACI	CCGC	4 cut(s)	301, 827, 1110, 1543
BspANI	GGCC	5 cut(s)	181, 473, 779, 1080, 1700
BspCNI	CTCAG	4 cut(s)	259, 344, 1117, 1381
BspHI	TCATGA	2 cut(s)	577, 633
BspLI	GGNNCC	5 cut(s)	473, 778, 957, 990, 1079
BspMAI	CTGCAG	2 cut(s)	25, 1414
BspMI	ACCTGC	1 cut(s)	1372
BspPI	GGATC	2 cut(s)	983, 996
BspTI	CTTAAG	1 cut(s)	250
BspTNI	GGTCTC	1 cut(s)	1147
BsrBI	CCGCTC	1 cut(s)	301
BsrI	ACTGG	3 cut(s)	780, 992, 1197
BssECI	CCNNGG	4 cut(s)	157, 407, 474, 1465
BssMI	GATC	6 cut(s)	140, 580, 988, 1231, 1525, 1537
BssNI	GRCGYC	1 cut(s)	510
BssSI	CACGAG	2 cut(s)	1377, 1424
BssT1I	CCWWGG	1 cut(s)	157
Bst2BI	CACGAG	2 cut(s)	1377, 1424
Bst2UI	CCWGG	6 cut(s)	408, 476, 1220, 1341, 1467, 1584
Bst4CI	ACNGT	2 cut(s)	917, 1612
BstACI	GRCGYC	1 cut(s)	510
BstAFI	CTTAAG	1 cut(s)	250
BstAPI	GCANNNNNTGC	1 cut(s)	134
BstC8I	GCNNGC	5 cut(s)	6, 10, 276, 1101, 1165
BstDEI	CTNAG	7 cut(s)	56, 86, 267, 331, 950, 1104, 1389
BstF5I	GGATG	4 cut(s)	153, 182, 484, 835
BstKTI	GATC	6 cut(s)	143, 583, 991, 1234, 1528, 1540
BstMAI	GTCTC	4 cut(s)	383, 627, 951, 1147
BstMBI	GATC	6 cut(s)	140, 580, 988, 1231, 1525, 1537
BstMWI	GCNNNNNNNGC	7 cut(s)	52, 134, 479, 833, 839, 1109, 1448
BstNI	CCWGG	6 cut(s)	408, 476, 1220, 1341, 1467, 1584
BstNSI	RCATGY	4 cut(s)	206, 730, 1103, 1499
BstPAI	GACNNNNGTC	2 cut(s)	619, 1550
BstSCI	CCNGG	8 cut(s)	406, 474, 1039, 1218, 1315, 1339, 1465, 1582
BstSFI	CTRYAG	2 cut(s)	21, 1410
BstSLI	GKGCMC	2 cut(s)	475, 1624
BstV1I	GCAGC	4 cut(s)	7, 77, 820, 871
BstX2I	RGATCY	3 cut(s)	140, 988, 1537
BstXI	CCANNNNNNTGG	2 cut(s)	712, 1073
BstYI	RGATCY	3 cut(s)	140, 988, 1537
Bsu36I	CCTNAGG	1 cut(s)	267
BsuRI	GGCC	5 cut(s)	181, 473, 779, 1080, 1700
BtsCI	GGATG	4 cut(s)	153, 182, 484, 835
BtsI	GCAGTG	1 cut(s)	19
BtsIMutI	CAGTG	6 cut(s)	19, 204, 787, 1190, 1386, 1608
BveI	ACCTGC	1 cut(s)	1372
Cac8I	GCNNGC	5 cut(s)	6, 10, 276, 1101, 1165
CciI	TCATGA	2 cut(s)	577, 633
Cfr13I	GGNCC	9 cut(s)	471, 472, 777, 956, 1055, 1078, 1662, 1673, 1698
CseI	GACGC	2 cut(s)	499, 1226
CsiI	ACCWGGT	1 cut(s)	1218
Csp6I	GTAC	2 cut(s)	418, 1313
CviQI	GTAC	2 cut(s)	418, 1313
DdeI	CTNAG	7 cut(s)	56, 86, 267, 331, 950, 1104, 1389
DpnI	GATC	6 cut(s)	142, 582, 990, 1233, 1527, 1539
DpnII	GATC	6 cut(s)	140, 580, 988, 1231, 1525, 1537
DraI	TTTAAA	1 cut(s)	1491
Eco130I	CCWWGG	1 cut(s)	157
Eco24I	GRGCYC	2 cut(s)	475, 1159
Eco31I	GGTCTC	1 cut(s)	1147
Eco47I	GGWCC	4 cut(s)	956, 1055, 1662, 1673
Eco57I	CTGAAG	1 cut(s)	180
Eco81I	CCTNAGG	1 cut(s)	267
Eco88I	CYCGRG	2 cut(s)	792, 1260
EcoO109I	RGGNCCY	3 cut(s)	471, 472, 1078
EcoRI	GAATTC	2 cut(s)	1306, 1511
EcoRII	CCWGG	6 cut(s)	406, 474, 1218, 1339, 1465, 1582
EcoT14I	CCWWGG	1 cut(s)	157
EcoT22I	ATGCAT	1 cut(s)	484
EcoT38I	GRGCYC	2 cut(s)	475, 1159
ErhI	CCWWGG	1 cut(s)	157
FalI	AAGNNNNNCTT	2 cut(s)	102, 134
FaqI	GGGAC	2 cut(s)	627, 1224
Fnu4HI	GCNGC	5 cut(s)	21, 66, 302, 834, 885
FokI	GGATG	4 cut(s)	140, 169, 491, 842
FriOI	GRGCYC	2 cut(s)	475, 1159
Fsp4HI	GCNGC	5 cut(s)	21, 66, 302, 834, 885
FspBI	CTAG	2 cut(s)	138, 491
GluI	GCNGC	5 cut(s)	21, 66, 302, 834, 885
GsaI	CCCAGC	1 cut(s)	177
GsuI	CTGGAG	1 cut(s)	522
HaeIII	GGCC	5 cut(s)	181, 473, 779, 1080, 1700
HapII	CCGG	3 cut(s)	191, 1040, 1317
HgaI	GACGC	2 cut(s)	499, 1226
Hin1I	GRCGYC	1 cut(s)	510
HinfI	GANTC	4 cut(s)	637, 677, 701, 1383
HpaII	CCGG	3 cut(s)	191, 1040, 1317
HphI	GGTGA	2 cut(s)	479, 1331
Hpy166II	GTNNAC	2 cut(s)	115, 1622
Hpy188I	TCNGA	3 cut(s)	334, 397, 1537
Hpy188III	TCNNGA	8 cut(s)	578, 634, 657, 794, 982, 1277, 1424, 1576
Hpy8I	GTNNAC	2 cut(s)	115, 1622
HpyAV	CCTTC	2 cut(s)	392, 979
HpyCH4III	ACNGT	2 cut(s)	917, 1612
HpyF10VI	GCNNNNNNNGC	7 cut(s)	52, 134, 479, 833, 839, 1109, 1448
HpyF3I	CTNAG	7 cut(s)	56, 86, 267, 331, 950, 1104, 1389
Hsp92I	GRCGYC	1 cut(s)	510
Kzo9I	GATC	6 cut(s)	140, 580, 988, 1231, 1525, 1537
LmnI	GCTCC	3 cut(s)	17, 62, 1482
Lsp1109I	GCAGC	4 cut(s)	7, 77, 820, 871
LweI	GCATC	4 cut(s)	469, 820, 1438, 1569
MabI	ACCWGGT	1 cut(s)	1218
MaeI	CTAG	2 cut(s)	138, 491
MaeIII	GTNAC	1 cut(s)	925
MalI	GATC	6 cut(s)	142, 582, 990, 1233, 1527, 1539
MbiI	CCGCTC	1 cut(s)	301
MboI	GATC	6 cut(s)	140, 580, 988, 1231, 1525, 1537
MboII	GAAGA	5 cut(s)	832, 970, 1622, 1657, 1660
MflI	RGATCY	3 cut(s)	140, 988, 1537
MhlI	GDGCHC	6 cut(s)	438, 475, 1159, 1261, 1333, 1624
MlyI	GAGTC	2 cut(s)	631, 686
MmeI	TCCRAC	1 cut(s)	1651
Mph1103I	ATGCAT	1 cut(s)	484
MroXI	GAANNNNTTC	1 cut(s)	1397
MseI	TTAA	5 cut(s)	120, 251, 1119, 1299, 1490
MslI	CAYNNNNRTG	5 cut(s)	441, 492, 870, 1428, 1693
MspCI	CTTAAG	1 cut(s)	250
MspI	CCGG	3 cut(s)	191, 1040, 1317
MspR9I	CCNGG	8 cut(s)	408, 476, 1041, 1220, 1317, 1341, 1467, 1584
MvaI	CCWGG	6 cut(s)	408, 476, 1220, 1341, 1467, 1584
MwoI	GCNNNNNNNGC	7 cut(s)	52, 134, 479, 833, 839, 1109, 1448
NciI	CCSGG	2 cut(s)	1041, 1317
NdeII	GATC	6 cut(s)	140, 580, 988, 1231, 1525, 1537
NlaIV	GGNNCC	5 cut(s)	473, 778, 957, 990, 1079
NmuCI	GTSAC	1 cut(s)	925
NsiI	ATGCAT	1 cut(s)	484
NspI	RCATGY	4 cut(s)	206, 730, 1103, 1499
OliI	CACNNNNGTG	2 cut(s)	870, 1428
PaeI	GCATGC	1 cut(s)	1103
PaeR7I	CTCGAG	1 cut(s)	792
PagI	TCATGA	2 cut(s)	577, 633
PaqCI	CACCTGC	1 cut(s)	1372
PdmI	GAANNNNTTC	1 cut(s)	1397
PfeI	GAWTC	2 cut(s)	701, 1383
PflMI	CCANNNNNTGG	3 cut(s)	530, 965, 998
PkrI	GCNGC	5 cut(s)	22, 67, 303, 835, 886
PleI	GAGTC	2 cut(s)	631, 685
PpsI	GAGTC	2 cut(s)	631, 685
PshAI	GACNNNNGTC	2 cut(s)	619, 1550
Psp6I	CCWGG	6 cut(s)	406, 474, 1218, 1339, 1465, 1582
PspFI	CCCAGC	1 cut(s)	173
PspGI	CCWGG	6 cut(s)	406, 474, 1218, 1339, 1465, 1582
PspN4I	GGNNCC	5 cut(s)	473, 778, 957, 990, 1079
PspOMI	GGGCCC	1 cut(s)	471
PspPI	GGNCC	9 cut(s)	471, 472, 777, 956, 1055, 1078, 1662, 1673, 1698
PstI	CTGCAG	2 cut(s)	25, 1414
PsuI	RGATCY	3 cut(s)	140, 988, 1537
RsaI	GTAC	2 cut(s)	419, 1314
RsaNI	GTAC	2 cut(s)	418, 1313
RseI	CAYNNNNRTG	5 cut(s)	441, 492, 870, 1428, 1693
SaqAI	TTAA	5 cut(s)	120, 251, 1119, 1299, 1490
SatI	GCNGC	5 cut(s)	21, 66, 302, 834, 885
Sau3AI	GATC	6 cut(s)	140, 580, 988, 1231, 1525, 1537
Sau96I	GGNCC	9 cut(s)	471, 472, 777, 956, 1055, 1078, 1662, 1673, 1698
SchI	GAGTC	2 cut(s)	631, 686
ScrFI	CCNGG	8 cut(s)	408, 476, 1041, 1220, 1317, 1341, 1467, 1584
SduI	GDGCHC	6 cut(s)	438, 475, 1159, 1261, 1333, 1624
SexAI	ACCWGGT	1 cut(s)	1218
SfaNI	GCATC	4 cut(s)	469, 820, 1438, 1569
SfcI	CTRYAG	2 cut(s)	21, 1410
Sfr274I	CTCGAG	1 cut(s)	792
SinI	GGWCC	4 cut(s)	956, 1055, 1662, 1673
SlaI	CTCGAG	1 cut(s)	792
SmiMI	CAYNNNNRTG	5 cut(s)	441, 492, 870, 1428, 1693
SmlI	CTYRAG	4 cut(s)	250, 317, 731, 792
SmoI	CTYRAG	4 cut(s)	250, 317, 731, 792
SphI	GCATGC	1 cut(s)	1103
SsiI	CCGC	4 cut(s)	301, 827, 1110, 1543
SspI	AATATT	1 cut(s)	1683
SspMI	CTAG	2 cut(s)	138, 491
StyD4I	CCNGG	8 cut(s)	406, 474, 1039, 1218, 1315, 1339, 1465, 1582
StyI	CCWWGG	1 cut(s)	157
TaaI	ACNGT	2 cut(s)	917, 1612
TaqI	TCGA	6 cut(s)	549, 754, 793, 1230, 1528, 1554
TauI	GCSGC	1 cut(s)	304
TfiI	GAWTC	2 cut(s)	701, 1383
Tru1I	TTAA	5 cut(s)	120, 251, 1119, 1299, 1490
Tru9I	TTAA	5 cut(s)	120, 251, 1119, 1299, 1490
TscAI	CASTG	6 cut(s)	19, 204, 787, 1197, 1393, 1615
TseFI	GTSAC	1 cut(s)	925
TseI	GCWGC	4 cut(s)	20, 65, 833, 884
Tsp45I	GTSAC	1 cut(s)	925
TspDTI	ATGAA	4 cut(s)	622, 714, 1390, 1658
TspRI	CASTG	6 cut(s)	19, 204, 787, 1197, 1393, 1615
Van91I	CCANNNNNTGG	3 cut(s)	530, 965, 998
Vha464I	CTTAAG	1 cut(s)	250
VneI	GTGCAC	1 cut(s)	1620
VpaK11BI	GGWCC	4 cut(s)	956, 1055, 1662, 1673
XapI	RAATTY	6 cut(s)	212, 337, 1115, 1272, 1306, 1511
XceI	RCATGY	4 cut(s)	206, 730, 1103, 1499
XhoI	CTCGAG	1 cut(s)	792
XmnI	GAANNNNTTC	1 cut(s)	1397
XspI	CTAG	2 cut(s)	138, 491
Zsp2I	ATGCAT	1 cut(s)	484

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.