Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0009132

Physical Location & Seq

Genomic Coordinates Reverse (-)

1317329 .. 1320981

Gene Structure 3653 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1009100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1605 bp

ATGCAGGCTTGTGGTGGAGCTGCAGCGATGGGTTCTCTTCAACAACCCACTTGGACAAAGGGAATAGCTTTTCCCAACAAAGGGCTTAGCTTTACCGGATATTCACATCAGCTGAAGCTCAATCGTGTGAAGCCCGGTACCGGTACTGCTAGATATTCTTCTTACATTGAAGGAAGCTTGACTGCTGGAAGGCCATCTTCTTCTGTCTCCGTGCCCTTGCCTGAAATCGGAGGTAGTGGAAGCAGTTTTGTGGACAATGGGTTAAGTGAGGCTGACCCTGAGGTACGTGCAATTATTACAAAGGAGAAGGAGCGTCAGCTCAAGAGCCTTGAGCTTATTGCCTCTGAAAATTTTACTTACCGGGCAGTGATGGAGGCAGTCGGCTCATGTCTCACAAACAAGTATTCAGAAGGATTACCAGGTAAAAGGTACTACGGTGGCAATGAGCACATTGATGAGCTTGAAACACTATGCCAAAAAAGGGCTTTGGATGCATTTCACTTAGACGGAAAGAAGTGGGGTGTTAATGTTCAACCATTATCTGGTTCTCCTGCTAATTTCGAAGTTTATACAGCAATTCTTAAACCTCATGACCGTCTCATGGGTTTGGACTTGTCTCATGGGGGACATTTGTCTCATGGTTTCATGACTCCTAAACGGCGGGTATCAGGCACATCCATATATTTTGAGTCCATGTCTTATCGACTCAATGAATCCACAGGTCTGGTTGATTATGACAAGCTTGAGGAAATGGCTGACCGATTTAAACCAAAACTCATTATTGCTGGGGCTAGTGCTTATCCTCGAGATTTTGACTATCCTCGCATGAGGAAGATCGCGGATGCTGTTGGTGCTTTTCTTATGATGGACATGGCTCACATAAGTGGGCTTGTTGCTGCTTCTGTGCTTGCAAATCCTTTTGACTACTGCGATATTGTGACCACAACAACACACAAGTCTTTAAGAGGCCCAAGAGGTGGAATGATCTTCTTCAAGAAAGATCCGGTTCTTGGGGTCGATTTAGAATCTGCCATCAACAATGCTGTTTTTCCAGGTTTACAGGGTGGTCCTCATAACCACACTATCGGGGGCCTTGCAGTTTGCCTGAAGTATGCACAGTGCCCAGAATTTAAGGCTTACCAGAACCAGGTGGTCTCTAATTGTAGGGCTCTTGCAAGTCGATTGACTGAACTTGGATACAAACTAGTTTCTGGTGGAAGCGATAACCATCTGGTTCTTGTGGATCTGCGGCCTTTAGGTATTGATGGAGCTCGAGTAGAGAAAATTCTTGACATGGCTTCTATCACCCTTAACAAGAATTCAGTACCCGGTGATAAGAGTGCTGTAGTGCCGGGCGGCATTCGCATTGGATCGCCTGCCATGACGACGAGAGGGTTCACAGAGAAAGAATTCGTAGCAGTTGCCGACTACATTCATGAGGGCGTGCAGATAACAATAGATGCCAAACGCACGGTTCTAGGACCGAAGCTCCAAGACTTTATGAAGTTTGTAGCTTCCCCGGAGTTCTCTCTAAAGGATCGAGTGTCCGATCTGCAGACAAGAGTTGAAGCTCTTACCACCCGGTTCCCAATGCCTGGCGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004372 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0016740 GO:0016741 GO:0016742 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00260 ko00460 ko00630 ko00670 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko01523 map00260 map00460 map00630 map00670 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map01523

Pfam Domains

Protein Families

SHMT

Protein Analysis

535

Amino Acids

57.93

Weight (kDa)

8.62

Isoelectric Point (pI)

32.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SHMT	PF00464	88 - 478	3.5e-192	Serine hydroxymethyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009132)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G32520 AT4G32520
fragaria_vesca	FvH4_7g07540 FvH4_7g07540 FvH4_7g07540
malus_domestica	MD09G1269900.v1.1 MD10G1009100.v1.1 MD17G1267800.v1.1
prunus_persica	Prupe.3G165800_v2.0.a1 Prupe.8G013000_v2.0.a1 Prupe.8G013000_v2.0.a1 Prupe.8G013000_v2.0.a1
pyrus_communis	pycom09g18110 pycom10g00550
rosa_chinensis	RchiOBHm_Chr7g0222651
rosa_laevigata	RLG00000002041
rosa_multiflora	Rmu_sc0001498.1_g000007
rosa_roxburghii	Rroxscaffold_3G00236570
rosa_rugosa	Rorug07G0211700
rosa_samantha	Rh7AG355300 Rh7BG343700
rosa_wichuraiana	Rw7G030180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	137
AccB1I	GGYRCC	1 cut(s)	137
AccB7I	CCANNNNNTGG	3 cut(s)	542, 977, 1595
AccII	CGCG	1 cut(s)	839
AciI	CCGC	4 cut(s)	661, 839, 1249, 1356
AclWI	GGATC	4 cut(s)	995, 1251, 1378, 1545
AcsI	RAATTY	5 cut(s)	349, 1127, 1284, 1318, 1409
AcuI	CTGAAG	2 cut(s)	134, 1127
AfaI	GTAC	5 cut(s)	139, 145, 285, 431, 1326
AgeI	ACCGGT	1 cut(s)	140
AgsI	TTSAA	6 cut(s)	41, 170, 464, 533, 994, 1568
AhlI	ACTAGT	1 cut(s)	1204
AjnI	CCWGG	4 cut(s)	418, 1051, 1146, 1594
AleI	CACNNNNGTG	1 cut(s)	882
Alw21I	GWGCWC	2 cut(s)	450, 1273
Alw26I	GTCTC	6 cut(s)	211, 395, 602, 621, 639, 1159
AlwI	GGATC	4 cut(s)	995, 1251, 1378, 1545
Ama87I	CYCGRG	2 cut(s)	804, 1272
AoxI	GGCC	4 cut(s)	191, 967, 1090, 1250
ApeKI	GCWGC	3 cut(s)	20, 23, 896
ApoI	RAATTY	5 cut(s)	349, 1127, 1284, 1318, 1409
AsiGI	ACCGGT	1 cut(s)	140
Asp700I	GAANNNNTTC	1 cut(s)	1409
Asp718I	GGTACC	1 cut(s)	137
AspS9I	GGNCC	4 cut(s)	968, 1067, 1090, 1481
AsuC2I	CCSGG	6 cut(s)	135, 362, 1329, 1353, 1520, 1582
AsuHPI	GGTGA	2 cut(s)	1297, 1343
AsuII	TTCGAA	1 cut(s)	561
AvaI	CYCGRG	2 cut(s)	804, 1272
AvaII	GGWCC	2 cut(s)	1067, 1481
AxyI	CCTNAGG	1 cut(s)	279
BaeGI	GKGCMC	2 cut(s)	216, 1124
BaeI	ACNNNNGTAYC	2 cut(s)	421, 454
BanI	GGYRCC	1 cut(s)	137
BanII	GRGCYC	2 cut(s)	1171, 1273
Bbv12I	GWGCWC	2 cut(s)	450, 1273
BbvI	GCAGC	3 cut(s)	7, 35, 883
BccI	CCATC	7 cut(s)	22, 202, 364, 859, 1040, 1236, 1259
BceAI	ACGGC	1 cut(s)	674
BciT130I	CCWGG	4 cut(s)	420, 1053, 1148, 1596
BciVI	GTATCC	1 cut(s)	1190
BcnI	CCSGG	6 cut(s)	135, 362, 1329, 1353, 1520, 1582
BcoDI	GTCTC	6 cut(s)	211, 395, 602, 621, 639, 1159
BcuI	ACTAGT	1 cut(s)	1204
BfaI	CTAG	4 cut(s)	150, 792, 1205, 1478
BfmI	CTRYAG	3 cut(s)	21, 1344, 1553
BfuI	GTATCC	1 cut(s)	1190
BisI	GCNGC	5 cut(s)	21, 24, 897, 1250, 1357
BlpI	GCTNAGC	1 cut(s)	86
BlsI	GCNGC	5 cut(s)	22, 25, 898, 1251, 1358
Bme18I	GGWCC	2 cut(s)	1067, 1481
BmeT110I	CYCGRG	2 cut(s)	804, 1272
BmgT120I	GGNCC	4 cut(s)	968, 1067, 1090, 1481
BmiI	GGNNCC	3 cut(s)	139, 1091, 1586
BmsI	GCATC	3 cut(s)	481, 832, 1450
BoxI	GACNNNNGTC	1 cut(s)	631
Bpu1102I	GCTNAGC	1 cut(s)	86
Bpu14I	TTCGAA	1 cut(s)	561
BpuEI	CTTGAG	3 cut(s)	305, 350, 764
BpuMI	CCSGG	6 cut(s)	135, 362, 1329, 1353, 1520, 1582
BsaAI	YACGTR	1 cut(s)	287
BsaBI	GATNNNNATC	1 cut(s)	1227
BsaI	GGTCTC	1 cut(s)	1159
BsaJI	CCNNGG	1 cut(s)	1518
BsaWI	WCCGGW	3 cut(s)	95, 140, 1003
BsaXI	ACNNNNNCTCC	4 cut(s)	1260, 1290, 1473, 1503
Bse118I	RCCGGY	1 cut(s)	140
Bse21I	CCTNAGG	1 cut(s)	279
Bse3DI	GCAATG	1 cut(s)	448
Bse8I	GATNNNNATC	1 cut(s)	1227
BseBI	CCWGG	4 cut(s)	420, 1053, 1148, 1596
BseDI	CCNNGG	1 cut(s)	1518
BseGI	GGATG	3 cut(s)	496, 674, 847
BseJI	GATNNNNATC	1 cut(s)	1227
BseMI	GCAATG	1 cut(s)	448
BseMII	CTCAG	1 cut(s)	270
BseSI	GKGCMC	2 cut(s)	216, 1124
BseXI	GCAGC	3 cut(s)	7, 35, 883
BseYI	CCCAGC	1 cut(s)	785
BsgI	GTGCAG	1 cut(s)	1466
Bsh1236I	CGCG	1 cut(s)	839
BshFI	GGCC	4 cut(s)	193, 969, 1092, 1252
BshNI	GGYRCC	1 cut(s)	137
BshTI	ACCGGT	1 cut(s)	140
BsiHKAI	GWGCWC	2 cut(s)	450, 1273
BsiHKCI	CYCGRG	2 cut(s)	804, 1272
BsiSI	CCGG	9 cut(s)	96, 135, 141, 361, 1004, 1329, 1352, 1520, 1582
BslFI	GGGAC	1 cut(s)	639
BsmAI	GTCTC	6 cut(s)	211, 395, 602, 621, 639, 1159
BsmBI	CGTCTC	1 cut(s)	602
BsmFI	GGGAC	1 cut(s)	639
BsmI	GAATGC	1 cut(s)	1359
BsnI	GGCC	4 cut(s)	193, 969, 1092, 1252
Bso31I	GGTCTC	1 cut(s)	1159
BsoBI	CYCGRG	2 cut(s)	804, 1272
Bsp119I	TTCGAA	1 cut(s)	561
Bsp1286I	GDGCHC	5 cut(s)	216, 450, 1124, 1171, 1273
Bsp143I	GATC	7 cut(s)	834, 984, 1000, 1243, 1370, 1537, 1549
Bsp1720I	GCTNAGC	1 cut(s)	86
BspACI	CCGC	4 cut(s)	661, 839, 1249, 1356
BspANI	GGCC	4 cut(s)	193, 969, 1092, 1252
BspCNI	CTCAG	1 cut(s)	271
BspFNI	CGCG	1 cut(s)	839
BspHI	TCATGA	3 cut(s)	589, 645, 1435
BspLI	GGNNCC	3 cut(s)	139, 1091, 1586
BspMAI	CTGCAG	2 cut(s)	25, 1557
BspPI	GGATC	4 cut(s)	995, 1251, 1378, 1545
BspT104I	TTCGAA	1 cut(s)	561
BspT107I	GGYRCC	1 cut(s)	137
BspTNI	GGTCTC	1 cut(s)	1159
BsrDI	GCAATG	1 cut(s)	448
BsrFI	RCCGGY	1 cut(s)	140
BssAI	RCCGGY	1 cut(s)	140
BssECI	CCNNGG	1 cut(s)	1518
BssMI	GATC	7 cut(s)	834, 984, 1000, 1243, 1370, 1537, 1549
Bst2UI	CCWGG	4 cut(s)	420, 1053, 1148, 1596
Bst4CI	ACNGT	4 cut(s)	437, 596, 1119, 1474
Bst6I	CTCTTC	1 cut(s)	42
BstBAI	YACGTR	1 cut(s)	287
BstBI	TTCGAA	1 cut(s)	561
BstC8I	GCNNGC	4 cut(s)	6, 909, 1377, 1445
BstDEI	CTNAG	3 cut(s)	86, 279, 502
BstF5I	GGATG	3 cut(s)	496, 674, 847
BstFNI	CGCG	1 cut(s)	839
BstKTI	GATC	7 cut(s)	837, 987, 1003, 1246, 1373, 1540, 1552
BstMAI	GTCTC	6 cut(s)	211, 395, 602, 621, 639, 1159
BstMBI	GATC	7 cut(s)	834, 984, 1000, 1243, 1370, 1537, 1549
BstMWI	GCNNNNNNNGC	3 cut(s)	491, 851, 1362
BstNI	CCWGG	4 cut(s)	420, 1053, 1148, 1596
BstPAI	GACNNNNGTC	1 cut(s)	631
BstSFI	CTRYAG	3 cut(s)	21, 1344, 1553
BstSLI	GKGCMC	2 cut(s)	216, 1124
BstUI	CGCG	1 cut(s)	839
BstV1I	GCAGC	3 cut(s)	7, 35, 883
BstX2I	RGATCY	2 cut(s)	1000, 1243
BstXI	CCANNNNNNTGG	1 cut(s)	724
BstYI	RGATCY	2 cut(s)	1000, 1243
Bsu36I	CCTNAGG	1 cut(s)	279
BsuI	GTATCC	1 cut(s)	1190
BsuRI	GGCC	4 cut(s)	193, 969, 1092, 1252
BtgZI	GCGATG	1 cut(s)	41
BtsCI	GGATG	3 cut(s)	496, 674, 847
BtsI	GCAGTG	1 cut(s)	372
BtsIMutI	CAGTG	2 cut(s)	372, 1124
Cac8I	GCNNGC	4 cut(s)	6, 909, 1377, 1445
CciI	TCATGA	3 cut(s)	589, 645, 1435
Cfr10I	RCCGGY	1 cut(s)	140
Cfr13I	GGNCC	4 cut(s)	968, 1067, 1090, 1481
CseI	GACGC	1 cut(s)	302
CsiI	ACCWGGT	2 cut(s)	418, 1146
Csp6I	GTAC	5 cut(s)	138, 144, 284, 430, 1325
CspAI	ACCGGT	1 cut(s)	140
CviQI	GTAC	5 cut(s)	138, 144, 284, 430, 1325
DdeI	CTNAG	3 cut(s)	86, 279, 502
DpnI	GATC	7 cut(s)	836, 986, 1002, 1245, 1372, 1539, 1551
DpnII	GATC	7 cut(s)	834, 984, 1000, 1243, 1370, 1537, 1549
DraI	TTTAAA	1 cut(s)	766
Eam1104I	CTCTTC	1 cut(s)	42
EarI	CTCTTC	1 cut(s)	42
Ecl136II	GAGCTC	1 cut(s)	1271
Eco24I	GRGCYC	2 cut(s)	1171, 1273
Eco31I	GGTCTC	1 cut(s)	1159
Eco47I	GGWCC	2 cut(s)	1067, 1481
Eco53kI	GAGCTC	1 cut(s)	1271
Eco57I	CTGAAG	2 cut(s)	134, 1127
Eco81I	CCTNAGG	1 cut(s)	279
Eco88I	CYCGRG	2 cut(s)	804, 1272
EcoICRI	GAGCTC	1 cut(s)	1271
EcoO109I	RGGNCCY	1 cut(s)	1090
EcoRI	GAATTC	2 cut(s)	1318, 1409
EcoRII	CCWGG	4 cut(s)	418, 1051, 1146, 1594
EcoT22I	ATGCAT	1 cut(s)	496
EcoT38I	GRGCYC	2 cut(s)	1171, 1273
Esp3I	CGTCTC	1 cut(s)	602
FalI	AAGNNNNNCTT	2 cut(s)	181, 213
FaqI	GGGAC	1 cut(s)	639
FauI	CCCGC	1 cut(s)	654
Fnu4HI	GCNGC	5 cut(s)	21, 24, 897, 1250, 1357
FokI	GGATG	3 cut(s)	503, 661, 854
FriOI	GRGCYC	2 cut(s)	1171, 1273
Fsp4HI	GCNGC	5 cut(s)	21, 24, 897, 1250, 1357
FspBI	CTAG	4 cut(s)	150, 792, 1205, 1478
GluI	GCNGC	5 cut(s)	21, 24, 897, 1250, 1357
GsaI	CCCAGC	1 cut(s)	789
HaeIII	GGCC	4 cut(s)	193, 969, 1092, 1252
HapII	CCGG	9 cut(s)	96, 135, 141, 361, 1004, 1329, 1352, 1520, 1582
HgaI	GACGC	1 cut(s)	302
HindIII	AAGCTT	2 cut(s)	175, 740
HinfI	GANTC	5 cut(s)	649, 689, 705, 713, 1025
HpaII	CCGG	9 cut(s)	96, 135, 141, 361, 1004, 1329, 1352, 1520, 1582
HphI	GGTGA	2 cut(s)	1297, 1343
Hpy166II	GTNNAC	3 cut(s)	253, 1058, 1398
Hpy188I	TCNGA	4 cut(s)	230, 346, 409, 1549
Hpy188III	TCNNGA	7 cut(s)	322, 590, 646, 806, 994, 1289, 1436
Hpy8I	GTNNAC	3 cut(s)	253, 1058, 1398
Hpy99I	CGWCG	1 cut(s)	1390
HpyAV	CCTTC	4 cut(s)	164, 183, 301, 404
HpyCH4III	ACNGT	4 cut(s)	437, 596, 1119, 1474
HpyCH4IV	ACGT	1 cut(s)	286
HpyF10VI	GCNNNNNNNGC	3 cut(s)	491, 851, 1362
HpyF3I	CTNAG	3 cut(s)	86, 279, 502
HpySE526I	ACGT	1 cut(s)	286
KpnI	GGTACC	1 cut(s)	141
Kzo9I	GATC	7 cut(s)	834, 984, 1000, 1243, 1370, 1537, 1549
LmnI	GCTCC	4 cut(s)	17, 310, 1268, 1494
Lsp1109I	GCAGC	3 cut(s)	7, 35, 883
LweI	GCATC	3 cut(s)	481, 832, 1450
MabI	ACCWGGT	2 cut(s)	418, 1146
MaeI	CTAG	4 cut(s)	150, 792, 1205, 1478
MaeII	ACGT	1 cut(s)	286
MaeIII	GTNAC	1 cut(s)	937
MalI	GATC	7 cut(s)	836, 986, 1002, 1245, 1372, 1539, 1551
MboI	GATC	7 cut(s)	834, 984, 1000, 1243, 1370, 1537, 1549
MboII	GAAGA	7 cut(s)	29, 150, 189, 192, 844, 979, 982
MflI	RGATCY	2 cut(s)	1000, 1243
MhlI	GDGCHC	5 cut(s)	216, 450, 1124, 1171, 1273
MluCI	AATT	9 cut(s)	291, 349, 556, 576, 1127, 1159, 1284, 1318, 1409
MlyI	GAGTC	3 cut(s)	643, 698, 699
Mph1103I	ATGCAT	1 cut(s)	496
MroXI	GAANNNNTTC	1 cut(s)	1409
MseI	TTAA	7 cut(s)	263, 525, 582, 765, 962, 1131, 1311
MslI	CAYNNNNRTG	2 cut(s)	453, 882
MspA1I	CMGCKG	1 cut(s)	112
MspI	CCGG	9 cut(s)	96, 135, 141, 361, 1004, 1329, 1352, 1520, 1582
Mva1269I	GAATGC	1 cut(s)	1359
MvaI	CCWGG	4 cut(s)	420, 1053, 1148, 1596
MvnI	CGCG	1 cut(s)	839
MwoI	GCNNNNNNNGC	3 cut(s)	491, 851, 1362
NciI	CCSGG	6 cut(s)	135, 362, 1329, 1353, 1520, 1582
NdeII	GATC	7 cut(s)	834, 984, 1000, 1243, 1370, 1537, 1549
NlaIV	GGNNCC	3 cut(s)	139, 1091, 1586
NmuCI	GTSAC	1 cut(s)	937
NsiI	ATGCAT	1 cut(s)	496
NspV	TTCGAA	1 cut(s)	561
OliI	CACNNNNGTG	1 cut(s)	882
PaeR7I	CTCGAG	2 cut(s)	804, 1272
PagI	TCATGA	3 cut(s)	589, 645, 1435
PctI	GAATGC	1 cut(s)	1359
PdmI	GAANNNNTTC	1 cut(s)	1409
PfeI	GAWTC	2 cut(s)	713, 1025
PflMI	CCANNNNNTGG	3 cut(s)	542, 977, 1595
PinAI	ACCGGT	1 cut(s)	140
PkrI	GCNGC	5 cut(s)	22, 25, 898, 1251, 1358
PleI	GAGTC	3 cut(s)	643, 697, 699
PpsI	GAGTC	3 cut(s)	643, 697, 699
Ppu21I	YACGTR	1 cut(s)	287
PshAI	GACNNNNGTC	1 cut(s)	631
Psp124BI	GAGCTC	1 cut(s)	1273
Psp6I	CCWGG	4 cut(s)	418, 1051, 1146, 1594
PspFI	CCCAGC	1 cut(s)	785
PspGI	CCWGG	4 cut(s)	418, 1051, 1146, 1594
PspN4I	GGNNCC	3 cut(s)	139, 1091, 1586
PspPI	GGNCC	4 cut(s)	968, 1067, 1090, 1481
PspXI	VCTCGAGB	1 cut(s)	1272
PstI	CTGCAG	2 cut(s)	25, 1557
PsuI	RGATCY	2 cut(s)	1000, 1243
PvuII	CAGCTG	1 cut(s)	112
RsaI	GTAC	5 cut(s)	139, 145, 285, 431, 1326
RsaNI	GTAC	5 cut(s)	138, 144, 284, 430, 1325
RseI	CAYNNNNRTG	2 cut(s)	453, 882
SacI	GAGCTC	1 cut(s)	1273
SaqAI	TTAA	7 cut(s)	263, 525, 582, 765, 962, 1131, 1311
SatI	GCNGC	5 cut(s)	21, 24, 897, 1250, 1357
Sau3AI	GATC	7 cut(s)	834, 984, 1000, 1243, 1370, 1537, 1549
Sau96I	GGNCC	4 cut(s)	968, 1067, 1090, 1481
SchI	GAGTC	3 cut(s)	643, 698, 699
SduI	GDGCHC	5 cut(s)	216, 450, 1124, 1171, 1273
SexAI	ACCWGGT	2 cut(s)	418, 1146
SfaNI	GCATC	3 cut(s)	481, 832, 1450
SfcI	CTRYAG	3 cut(s)	21, 1344, 1553
Sfr274I	CTCGAG	2 cut(s)	804, 1272
SfuI	TTCGAA	1 cut(s)	561
SinI	GGWCC	2 cut(s)	1067, 1481
SlaI	CTCGAG	2 cut(s)	804, 1272
SmiMI	CAYNNNNRTG	2 cut(s)	453, 882
SmlI	CTYRAG	5 cut(s)	320, 329, 743, 804, 1272
SmoI	CTYRAG	5 cut(s)	320, 329, 743, 804, 1272
SpeI	ACTAGT	1 cut(s)	1204
Sse9I	AATT	9 cut(s)	291, 349, 556, 576, 1127, 1159, 1284, 1318, 1409
SsiI	CCGC	4 cut(s)	661, 839, 1249, 1356
SspMI	CTAG	4 cut(s)	150, 792, 1205, 1478
SstI	GAGCTC	1 cut(s)	1273
TaaI	ACNGT	4 cut(s)	437, 596, 1119, 1474
TaiI	ACGT	1 cut(s)	289
TaqI	TCGA	7 cut(s)	561, 703, 805, 1017, 1180, 1273, 1540
TaqII	GACCGA	2 cut(s)	774, 1498
TasI	AATT	9 cut(s)	291, 349, 556, 576, 1127, 1159, 1284, 1318, 1409
TauI	GCSGC	2 cut(s)	1252, 1359
TfiI	GAWTC	2 cut(s)	713, 1025
Tru1I	TTAA	7 cut(s)	263, 525, 582, 765, 962, 1131, 1311
Tru9I	TTAA	7 cut(s)	263, 525, 582, 765, 962, 1131, 1311
TscAI	CASTG	2 cut(s)	372, 1124
TseFI	GTSAC	1 cut(s)	937
TseI	GCWGC	3 cut(s)	20, 23, 896
Tsp45I	GTSAC	1 cut(s)	937
TspDTI	ATGAA	4 cut(s)	634, 726, 1424, 1517
TspGWI	ACGGA	2 cut(s)	199, 522
TspRI	CASTG	2 cut(s)	372, 1124
Van91I	CCANNNNNTGG	3 cut(s)	542, 977, 1595
VpaK11BI	GGWCC	2 cut(s)	1067, 1481
XapI	RAATTY	5 cut(s)	349, 1127, 1284, 1318, 1409
XhoI	CTCGAG	2 cut(s)	804, 1272
XmnI	GAANNNNTTC	1 cut(s)	1409
XspI	CTAG	4 cut(s)	150, 792, 1205, 1478
Zsp2I	ATGCAT	1 cut(s)	496

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.