MD09G1279700.v1.1

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0015584
Physical Location & Seq
Forward (+)
35654801 .. 35655805
1005 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1279700.v1.1.491

Sequence Viewer

Length: 663 bp
ATGTCGACGGAAGATGTTGTTCTTTTGGACTGCTGGGTTAGCCCTTTCTGCATGAGGGTGAAAATTGTGTTGGAGGAAAAGGGCATCCAATATGAAGAGAGAGCTGAAGATCTATTTGGAGGCAAGAGTGAGCTGCTCCTCACATCCAACCCGATTTACACCAAGGTGCCTGTGCTGCTTCACAAGGGAAAACCAGTGTGTGAGTCAAGCATCATTGTTGGCTACGTTGATGAGACCTGGGCTTCTCCACCATTGCTCCCATCTTGCCCTTATGCTCGTGCTCAGGCCAAGTTCCAGGCCGACTACATTGACAAAAAGCTGTACGATGCAGGAAGCAGTATATGGAGGACCAAAGGAGAAGCACAAGAGGTAGCTGTAAAAGACTTTATAGAAGTCTTAAAGCAGCTAGAGAAATTTCTCGGGGACAAAGATTTCTTCGGTGGAGATAGCTTTGGTTTTGTTGACATCATAACGATCGGTATAACGAGCTGGTTCTCTGCATATGAAAAGTTTGGAAACTTCAAGGTTGAAGACCACACACCAAAGTTGTCTGCTTGGATTAATAGGTGCATGCAGAGAAAGACAGTAGCCAAAGTCATTCCAGACCCAGAAAAAGTCTATGAATTCGTCATCAATCTCAAAAAGATGATGGGATTTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418
Pfam Domains
Protein Families
GST_C GST_C_2 GST_N

Protein Analysis

221

Amino Acids

25.03

Weight (kDa)

5.43

Isoelectric Point (pI)

30.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 7 - 78 2.6e-15 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 10 - 81 1.7e-13 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 14 - 78 2.2e-12 Glutathione S-transferase, N-terminal domain
GST_C PF00043 120 - 193 5.6e-10 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 122 - 189 3.5e-08 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015584)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g37420
malus_domestica MD09G1279700.v1.1 MD17G1271700.v1.1 MD17G1286500.v1.1
prunus_persica Prupe.3G014500_v2.0.a1
pyrus_communis pycom17g26990
rosa_chinensis RchiOBHm_Chr5g0067311
rosa_laevigata RLG00000035926
rosa_multiflora Rmu_sc0000015.1_g000002
rosa_roxburghii Rroxscaffold_1G00013760
rosa_rugosa Rorug05G0384100
rosa_samantha Rh5AG443500 Rh5BG460500 Rh5CG482800 Rh5DG475600
rosa_wichuraiana Rw5G041420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 166
AccI GTMKAC 1 cut(s) 5
AcsI RAATTY 2 cut(s) 413, 623
AcuI CTGAAG 1 cut(s) 126
AfaI GTAC 1 cut(s) 323
AgsI TTSAA 3 cut(s) 523, 530, 659
AjnI CCWGG 2 cut(s) 236, 294
AjuI GAANNNNNNNTTGG 4 cut(s) 53, 85, 99, 131
AleI CACNNNNGTG 1 cut(s) 164
AluBI AGCT 7 cut(s) 104, 133, 319, 374, 406, 450, 489
AluI AGCT 7 cut(s) 104, 133, 319, 374, 406, 450, 489
Alw21I GWGCWC 1 cut(s) 283
Alw26I GTCTC 1 cut(s) 227
Ama87I CYCGRG 1 cut(s) 419
AoxI GGCC 2 cut(s) 285, 297
ApeKI GCWGC 3 cut(s) 133, 175, 403
ApoI RAATTY 2 cut(s) 413, 623
AseI ATTAAT 1 cut(s) 561
AspS9I GGNCC 1 cut(s) 348
AsuHPI GGTGA 1 cut(s) 70
AvaI CYCGRG 1 cut(s) 419
AvaII GGWCC 1 cut(s) 348
BanI GGYRCC 1 cut(s) 166
BauI CACGAG 1 cut(s) 276
BbsI GAAGAC 1 cut(s) 537
Bbv12I GWGCWC 1 cut(s) 283
BbvI GCAGC 3 cut(s) 120, 162, 415
BccI CCATC 2 cut(s) 268, 643
BciT130I CCWGG 2 cut(s) 238, 296
BcoDI GTCTC 1 cut(s) 227
BfaI CTAG 1 cut(s) 407
BglII AGATCT 1 cut(s) 109
BisI GCNGC 3 cut(s) 134, 176, 404
BlsI GCNGC 3 cut(s) 135, 177, 405
Bme1390I CCNGG 2 cut(s) 238, 296
Bme18I GGWCC 1 cut(s) 348
BmeT110I CYCGRG 1 cut(s) 419
BmgT120I GGNCC 1 cut(s) 348
BmiI GGNNCC 1 cut(s) 168
BmrFI CCNGG 2 cut(s) 238, 296
BmsI GCATC 3 cut(s) 93, 219, 316
BpiI GAAGAC 1 cut(s) 537
Bpu10I CCTNAGC 1 cut(s) 282
BsaI GGTCTC 1 cut(s) 227
BsaJI CCNNGG 2 cut(s) 162, 237
BsaXI ACNNNNNCTCC 2 cut(s) 240, 270
Bse1I ACTGG 1 cut(s) 194
Bse3DI GCAATG 1 cut(s) 251
BseBI CCWGG 2 cut(s) 238, 296
BseDI CCNNGG 2 cut(s) 162, 237
BseGI GGATG 2 cut(s) 84, 143
BseMI GCAATG 1 cut(s) 251
BseMII CTCAG 1 cut(s) 296
BseNI ACTGG 1 cut(s) 194
BseRI GAGGAG 1 cut(s) 128
BseXI GCAGC 3 cut(s) 120, 162, 415
BseYI CCCAGC 1 cut(s) 33
Bsh1285I CGRYCG 1 cut(s) 477
BshFI GGCC 2 cut(s) 287, 299
BshNI GGYRCC 1 cut(s) 166
BsiEI CGRYCG 1 cut(s) 477
BsiHKAI GWGCWC 1 cut(s) 283
BsiHKCI CYCGRG 1 cut(s) 419
BslFI GGGAC 1 cut(s) 437
BsmAI GTCTC 1 cut(s) 227
BsmFI GGGAC 1 cut(s) 437
BsnI GGCC 2 cut(s) 287, 299
Bso31I GGTCTC 1 cut(s) 227
BsoBI CYCGRG 1 cut(s) 419
Bsp1286I GDGCHC 1 cut(s) 283
Bsp143I GATC 2 cut(s) 109, 474
BspANI GGCC 2 cut(s) 287, 299
BspCNI CTCAG 1 cut(s) 295
BspLI GGNNCC 1 cut(s) 168
BspT107I GGYRCC 1 cut(s) 166
BspTNI GGTCTC 1 cut(s) 227
BsrDI GCAATG 1 cut(s) 251
BsrI ACTGG 1 cut(s) 194
BssECI CCNNGG 2 cut(s) 162, 237
BssMI GATC 2 cut(s) 109, 474
BssSI CACGAG 1 cut(s) 276
BssT1I CCWWGG 1 cut(s) 162
Bst2BI CACGAG 1 cut(s) 276
Bst2UI CCWGG 2 cut(s) 238, 296
Bst4CI ACNGT 1 cut(s) 586
Bst6I CTCTTC 1 cut(s) 90
BstC8I GCNNGC 1 cut(s) 572
BstDEI CTNAG 1 cut(s) 282
BstF5I GGATG 2 cut(s) 84, 143
BstKTI GATC 2 cut(s) 112, 477
BstMAI GTCTC 1 cut(s) 227
BstMBI GATC 2 cut(s) 109, 474
BstMCI CGRYCG 1 cut(s) 477
BstMWI GCNNNNNNNGC 3 cut(s) 39, 48, 175
BstNI CCWGG 2 cut(s) 238, 296
BstNSI RCATGY 1 cut(s) 574
BstSCI CCNGG 2 cut(s) 236, 294
BstV1I GCAGC 3 cut(s) 120, 162, 415
BstV2I GAAGAC 1 cut(s) 537
BstX2I RGATCY 1 cut(s) 109
BstYI RGATCY 1 cut(s) 109
BsuRI GGCC 2 cut(s) 287, 299
BtsCI GGATG 2 cut(s) 84, 143
BtsIMutI CAGTG 1 cut(s) 201
Cac8I GCNNGC 1 cut(s) 572
Cfr13I GGNCC 1 cut(s) 348
Csp6I GTAC 1 cut(s) 322
CviAII CATG 2 cut(s) 52, 571
CviQI GTAC 1 cut(s) 322
DdeI CTNAG 1 cut(s) 282
DpnI GATC 2 cut(s) 111, 476
DpnII GATC 2 cut(s) 109, 474
Eam1104I CTCTTC 1 cut(s) 90
EarI CTCTTC 1 cut(s) 90
Eco130I CCWWGG 1 cut(s) 162
Eco31I GGTCTC 1 cut(s) 227
Eco47I GGWCC 1 cut(s) 348
Eco57I CTGAAG 1 cut(s) 126
Eco88I CYCGRG 1 cut(s) 419
EcoRI GAATTC 1 cut(s) 623
EcoRII CCWGG 2 cut(s) 236, 294
EcoT14I CCWWGG 1 cut(s) 162
ErhI CCWWGG 1 cut(s) 162
FaeI CATG 2 cut(s) 55, 574
FaqI GGGAC 1 cut(s) 437
FatI CATG 2 cut(s) 51, 570
FauNDI CATATG 1 cut(s) 502
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 3 cut(s) 134, 176, 404
FokI GGATG 2 cut(s) 71, 130
Fsp4HI GCNGC 3 cut(s) 134, 176, 404
FspBI CTAG 1 cut(s) 407
GluI GCNGC 3 cut(s) 134, 176, 404
GsaI CCCAGC 1 cut(s) 37
HaeIII GGCC 2 cut(s) 287, 299
Hin1II CATG 2 cut(s) 55, 574
HincII GTYRAC 2 cut(s) 6, 463
HindII GTYRAC 2 cut(s) 6, 463
HinfI GANTC 1 cut(s) 203
HphI GGTGA 1 cut(s) 70
Hpy166II GTNNAC 2 cut(s) 6, 463
Hpy188III TCNNGA 1 cut(s) 602
Hpy8I GTNNAC 2 cut(s) 6, 463
Hpy99I CGWCG 1 cut(s) 10
HpyCH4III ACNGT 1 cut(s) 586
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 5 cut(s) 51, 329, 500, 570, 574
HpyF10VI GCNNNNNNNGC 3 cut(s) 39, 48, 175
HpyF3I CTNAG 1 cut(s) 282
HpySE526I ACGT 1 cut(s) 225
Hsp92II CATG 2 cut(s) 55, 574
Kzo9I GATC 2 cut(s) 109, 474
LmnI GCTCC 2 cut(s) 141, 261
Lsp1109I GCAGC 3 cut(s) 120, 162, 415
LweI GCATC 3 cut(s) 93, 219, 316
MaeI CTAG 1 cut(s) 407
MaeII ACGT 1 cut(s) 225
MalI GATC 2 cut(s) 111, 476
MboI GATC 2 cut(s) 109, 474
MboII GAAGA 5 cut(s) 23, 107, 119, 427, 542
MflI RGATCY 1 cut(s) 109
MhlI GDGCHC 1 cut(s) 283
MluCI AATT 3 cut(s) 63, 413, 623
MlyI GAGTC 1 cut(s) 212
MmeI TCCRAC 2 cut(s) 51, 171
MnlI CCTC 6 cut(s) 48, 67, 113, 149, 339, 361
MseI TTAA 2 cut(s) 398, 561
MslI CAYNNNNRTG 2 cut(s) 56, 164
MspR9I CCNGG 2 cut(s) 238, 296
MvaI CCWGG 2 cut(s) 238, 296
MwoI GCNNNNNNNGC 3 cut(s) 39, 48, 175
NdeI CATATG 1 cut(s) 502
NdeII GATC 2 cut(s) 109, 474
NlaIII CATG 2 cut(s) 55, 574
NlaIV GGNNCC 1 cut(s) 168
NspI RCATGY 1 cut(s) 574
OliI CACNNNNGTG 1 cut(s) 164
PaeI GCATGC 1 cut(s) 574
PkrI GCNGC 3 cut(s) 135, 177, 405
Ple19I CGATCG 1 cut(s) 477
PleI GAGTC 1 cut(s) 211
PpsI GAGTC 1 cut(s) 211
PshBI ATTAAT 1 cut(s) 561
Psp6I CCWGG 2 cut(s) 236, 294
PspFI CCCAGC 1 cut(s) 33
PspGI CCWGG 2 cut(s) 236, 294
PspN4I GGNNCC 1 cut(s) 168
PspPI GGNCC 1 cut(s) 348
PsuI RGATCY 1 cut(s) 109
PvuI CGATCG 1 cut(s) 477
RsaI GTAC 1 cut(s) 323
RsaNI GTAC 1 cut(s) 322
RseI CAYNNNNRTG 2 cut(s) 56, 164
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 2 cut(s) 398, 561
SatI GCNGC 3 cut(s) 134, 176, 404
Sau3AI GATC 2 cut(s) 109, 474
Sau96I GGNCC 1 cut(s) 348
SchI GAGTC 1 cut(s) 212
ScrFI CCNGG 2 cut(s) 238, 296
SduI GDGCHC 1 cut(s) 283
SfaNI GCATC 3 cut(s) 93, 219, 316
SinI GGWCC 1 cut(s) 348
SmiMI CAYNNNNRTG 2 cut(s) 56, 164
SphI GCATGC 1 cut(s) 574
Sse9I AATT 3 cut(s) 63, 413, 623
SspMI CTAG 1 cut(s) 407
StyD4I CCNGG 2 cut(s) 236, 294
StyI CCWWGG 1 cut(s) 162
TaaI ACNGT 1 cut(s) 586
TaiI ACGT 1 cut(s) 228
TaqI TCGA 1 cut(s) 5
TasI AATT 3 cut(s) 63, 413, 623
Tru1I TTAA 2 cut(s) 398, 561
Tru9I TTAA 2 cut(s) 398, 561
TscAI CASTG 1 cut(s) 201
TseI GCWGC 3 cut(s) 133, 175, 403
TspDTI ATGAA 3 cut(s) 108, 519, 636
TspGWI ACGGA 1 cut(s) 23
TspRI CASTG 1 cut(s) 201
VpaK11BI GGWCC 1 cut(s) 348
VspI ATTAAT 1 cut(s) 561
XapI RAATTY 2 cut(s) 413, 623
XceI RCATGY 1 cut(s) 574
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.