MD17G1271700.v1.1

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0015584
Physical Location & Seq
Forward (+)
33239918 .. 33240984
1067 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1271700.v1.1.491

Sequence Viewer

Length: 663 bp
ATGGCGACGGAAGATGTTGTTCTCTTGGACCTCTGGGCTAGCCCGTACTGCATGAGGGTGAAGATTGCCTTGGAGGAAAAGGGCATCAAATATGAAGAGAGAGCTGAGGATCTCTTTGGAGGCAAGAGTGAACTGCTGCTCACATCCAACCCGGTTTACGCCAAGGTGCCCGTGCTTCTTCACAAGGGAAAACCATTGTGTGAGTCAAGCATCATCGTCGGCTACATTGATGAGACCTGGGCTTCCCCACCATTGCTCCCATCTTGCCCTTATGCTCGTGCTCAGGCCAAGTTCTGGGTCGACTACGTTGACAAGAAGCTGTTCGATGCAGGAGGCAATATATGGAGAACCAAAGGAGAAGCACAAGAGGTGGCTGTAAAAGACTTCATAGAAATCTTAAAGCAGCTAGAGAAAGTTCTTGGGGACAAAGATTTCTTCGGTGGCGATAGCTTTGGTTTCGTTGACATCATAACGATCGCTATAACAAGCTGGTTCCTTGCATTCGAAAAATTTGGAAACTTCAAGGTTGAAGACCACGCACCAAAGTTGTCAGCTTGGATTAAAAGGTCCATGCAGAGAGAGACAGTAGCCAAAGTCATCCCAGACCCAGAAAAAATCTATGAATTCGTCATCAACCTCAAAAAAATGATGGGGATGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418
Pfam Domains
Protein Families
GST_C GST_C_2 GST_N

Protein Analysis

221

Amino Acids

24.98

Weight (kDa)

5.44

Isoelectric Point (pI)

34.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 6 - 78 2.7e-16 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 12 - 81 2.4e-14 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 13 - 79 6.3e-13 Glutathione S-transferase, N-terminal domain
GST_C PF00043 120 - 194 7.8e-10 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 122 - 189 7.9e-08 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015584)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g37420
malus_domestica MD09G1279700.v1.1 MD17G1271700.v1.1 MD17G1286500.v1.1
prunus_persica Prupe.3G014500_v2.0.a1
pyrus_communis pycom17g26990
rosa_chinensis RchiOBHm_Chr5g0067311
rosa_laevigata RLG00000035926
rosa_multiflora Rmu_sc0000015.1_g000002
rosa_roxburghii Rroxscaffold_1G00013760
rosa_rugosa Rorug05G0384100
rosa_samantha Rh5AG443500 Rh5BG460500 Rh5CG482800 Rh5DG475600
rosa_wichuraiana Rw5G041420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 166
AccB7I CCANNNNNTGG 1 cut(s) 294
AccI GTMKAC 1 cut(s) 300
AclWI GGATC 1 cut(s) 117
AcsI RAATTY 2 cut(s) 509, 623
AfaI GTAC 1 cut(s) 47
AfiI CCNNNNNNNGG 1 cut(s) 294
AgsI TTSAA 2 cut(s) 523, 530
AjnI CCWGG 1 cut(s) 236
AjuI GAANNNNNNNTTGG 2 cut(s) 53, 85
AluBI AGCT 6 cut(s) 104, 319, 406, 450, 489, 554
AluI AGCT 6 cut(s) 104, 319, 406, 450, 489, 554
Alw21I GWGCWC 1 cut(s) 283
Alw26I GTCTC 2 cut(s) 227, 575
AlwI GGATC 1 cut(s) 117
AoxI GGCC 1 cut(s) 285
ApeKI GCWGC 2 cut(s) 136, 403
ApoI RAATTY 2 cut(s) 509, 623
Asp700I GAANNNNTTC 1 cut(s) 320
AspS9I GGNCC 2 cut(s) 28, 567
AsuC2I CCSGG 1 cut(s) 152
AsuHPI GGTGA 1 cut(s) 70
AsuII TTCGAA 1 cut(s) 504
AsuNHI GCTAGC 1 cut(s) 38
AvaII GGWCC 2 cut(s) 28, 567
BaeGI GKGCMC 1 cut(s) 171
BanI GGYRCC 1 cut(s) 166
BauI CACGAG 1 cut(s) 276
BbsI GAAGAC 1 cut(s) 537
Bbv12I GWGCWC 1 cut(s) 283
BbvCI CCTCAGC 1 cut(s) 105
BbvI GCAGC 2 cut(s) 123, 415
BccI CCATC 3 cut(s) 268, 643, 649
BcgI CGANNNNNNTGC 2 cut(s) 199, 233
BciT130I CCWGG 1 cut(s) 238
BcnI CCSGG 1 cut(s) 152
BcoDI GTCTC 2 cut(s) 227, 575
BfaI CTAG 2 cut(s) 39, 407
BisI GCNGC 2 cut(s) 137, 404
BlsI GCNGC 2 cut(s) 138, 405
Bme1390I CCNGG 2 cut(s) 152, 238
Bme18I GGWCC 2 cut(s) 28, 567
BmgT120I GGNCC 2 cut(s) 28, 567
BmiI GGNNCC 2 cut(s) 168, 494
BmrFI CCNGG 2 cut(s) 152, 238
BmsI GCATC 3 cut(s) 93, 219, 316
BmtI GCTAGC 1 cut(s) 42
BpiI GAAGAC 1 cut(s) 537
Bpu10I CCTNAGC 2 cut(s) 105, 282
Bpu14I TTCGAA 1 cut(s) 504
BpuMI CCSGG 1 cut(s) 152
BsaI GGTCTC 1 cut(s) 227
BsaJI CCNNGG 3 cut(s) 69, 162, 237
BsaXI ACNNNNNCTCC 2 cut(s) 240, 270
Bsc4I CCNNNNNNNGG 1 cut(s) 294
Bse3DI GCAATG 1 cut(s) 251
BseBI CCWGG 1 cut(s) 238
BseDI CCNNGG 3 cut(s) 69, 162, 237
BseGI GGATG 3 cut(s) 143, 597, 660
BseLI CCNNNNNNNGG 1 cut(s) 294
BseMI GCAATG 1 cut(s) 251
BseMII CTCAG 2 cut(s) 96, 296
BseSI GKGCMC 1 cut(s) 171
BseXI GCAGC 2 cut(s) 123, 415
Bsh1285I CGRYCG 1 cut(s) 477
BshFI GGCC 1 cut(s) 287
BshNI GGYRCC 1 cut(s) 166
BsiEI CGRYCG 1 cut(s) 477
BsiHKAI GWGCWC 1 cut(s) 283
BsiSI CCGG 1 cut(s) 152
BslFI GGGAC 1 cut(s) 437
BslI CCNNNNNNNGG 1 cut(s) 294
BsmAI GTCTC 2 cut(s) 227, 575
BsmFI GGGAC 1 cut(s) 437
BsmI GAATGC 1 cut(s) 500
BsnI GGCC 1 cut(s) 287
Bso31I GGTCTC 1 cut(s) 227
Bsp119I TTCGAA 1 cut(s) 504
Bsp1286I GDGCHC 2 cut(s) 171, 283
Bsp143I GATC 2 cut(s) 109, 474
BspANI GGCC 1 cut(s) 287
BspCNI CTCAG 2 cut(s) 97, 295
BspLI GGNNCC 2 cut(s) 168, 494
BspOI GCTAGC 1 cut(s) 42
BspPI GGATC 1 cut(s) 117
BspT104I TTCGAA 1 cut(s) 504
BspT107I GGYRCC 1 cut(s) 166
BspTNI GGTCTC 1 cut(s) 227
BsrDI GCAATG 1 cut(s) 251
BssECI CCNNGG 3 cut(s) 69, 162, 237
BssMI GATC 2 cut(s) 109, 474
BssSI CACGAG 1 cut(s) 276
BssT1I CCWWGG 2 cut(s) 69, 162
Bst2BI CACGAG 1 cut(s) 276
Bst2UI CCWGG 1 cut(s) 238
Bst4CI ACNGT 1 cut(s) 586
Bst6I CTCTTC 1 cut(s) 90
BstBI TTCGAA 1 cut(s) 504
BstC8I GCNNGC 1 cut(s) 40
BstDEI CTNAG 2 cut(s) 105, 282
BstF5I GGATG 3 cut(s) 143, 597, 660
BstKTI GATC 2 cut(s) 112, 477
BstMAI GTCTC 2 cut(s) 227, 575
BstMBI GATC 2 cut(s) 109, 474
BstMCI CGRYCG 1 cut(s) 477
BstMWI GCNNNNNNNGC 1 cut(s) 48
BstNI CCWGG 1 cut(s) 238
BstSCI CCNGG 2 cut(s) 150, 236
BstSLI GKGCMC 1 cut(s) 171
BstV1I GCAGC 2 cut(s) 123, 415
BstV2I GAAGAC 1 cut(s) 537
BstX2I RGATCY 1 cut(s) 109
BstYI RGATCY 1 cut(s) 109
BsuRI GGCC 1 cut(s) 287
BtsCI GGATG 3 cut(s) 143, 597, 660
Cac8I GCNNGC 1 cut(s) 40
Cfr13I GGNCC 2 cut(s) 28, 567
Csp6I GTAC 1 cut(s) 46
CviAII CATG 2 cut(s) 52, 571
CviQI GTAC 1 cut(s) 46
DdeI CTNAG 2 cut(s) 105, 282
DpnI GATC 2 cut(s) 111, 476
DpnII GATC 2 cut(s) 109, 474
Eam1104I CTCTTC 1 cut(s) 90
EarI CTCTTC 1 cut(s) 90
Eco130I CCWWGG 2 cut(s) 69, 162
Eco31I GGTCTC 1 cut(s) 227
Eco47I GGWCC 2 cut(s) 28, 567
EcoRI GAATTC 1 cut(s) 623
EcoRII CCWGG 1 cut(s) 236
EcoT14I CCWWGG 2 cut(s) 69, 162
ErhI CCWWGG 2 cut(s) 69, 162
FaeI CATG 2 cut(s) 55, 574
FalI AAGNNNNNCTT 2 cut(s) 53, 85
FaqI GGGAC 1 cut(s) 437
FatI CATG 2 cut(s) 51, 570
FblI GTMKAC 1 cut(s) 300
Fnu4HI GCNGC 2 cut(s) 137, 404
FokI GGATG 2 cut(s) 130, 584
Fsp4HI GCNGC 2 cut(s) 137, 404
FspBI CTAG 2 cut(s) 39, 407
GluI GCNGC 2 cut(s) 137, 404
HaeIII GGCC 1 cut(s) 287
HapII CCGG 1 cut(s) 152
Hin1II CATG 2 cut(s) 55, 574
HincII GTYRAC 3 cut(s) 301, 310, 463
HindII GTYRAC 3 cut(s) 301, 310, 463
HinfI GANTC 1 cut(s) 203
HpaII CCGG 1 cut(s) 152
HphI GGTGA 1 cut(s) 70
Hpy166II GTNNAC 5 cut(s) 131, 157, 301, 310, 463
Hpy8I GTNNAC 5 cut(s) 131, 157, 301, 310, 463
Hpy99I CGWCG 2 cut(s) 10, 221
HpyCH4III ACNGT 1 cut(s) 586
HpyCH4IV ACGT 1 cut(s) 306
HpyCH4V TGCA 4 cut(s) 51, 329, 500, 574
HpyF10VI GCNNNNNNNGC 1 cut(s) 48
HpyF3I CTNAG 2 cut(s) 105, 282
HpySE526I ACGT 1 cut(s) 306
Hsp92II CATG 2 cut(s) 55, 574
Kzo9I GATC 2 cut(s) 109, 474
LmnI GCTCC 1 cut(s) 261
Lsp1109I GCAGC 2 cut(s) 123, 415
LweI GCATC 3 cut(s) 93, 219, 316
MaeI CTAG 2 cut(s) 39, 407
MaeII ACGT 1 cut(s) 306
MalI GATC 2 cut(s) 111, 476
MboI GATC 2 cut(s) 109, 474
MboII GAAGA 6 cut(s) 23, 73, 107, 170, 427, 542
MflI RGATCY 1 cut(s) 109
MhlI GDGCHC 2 cut(s) 171, 283
MluCI AATT 2 cut(s) 509, 623
MlyI GAGTC 1 cut(s) 212
MmeI TCCRAC 1 cut(s) 171
MnlI CCTC 8 cut(s) 41, 48, 67, 100, 113, 326, 361, 647
MroXI GAANNNNTTC 1 cut(s) 320
MseI TTAA 2 cut(s) 398, 561
MslI CAYNNNNRTG 1 cut(s) 56
MspI CCGG 1 cut(s) 152
MspR9I CCNGG 2 cut(s) 152, 238
Mva1269I GAATGC 1 cut(s) 500
MvaI CCWGG 1 cut(s) 238
MwoI GCNNNNNNNGC 1 cut(s) 48
NciI CCSGG 1 cut(s) 152
NdeII GATC 2 cut(s) 109, 474
NheI GCTAGC 1 cut(s) 38
NlaIII CATG 2 cut(s) 55, 574
NlaIV GGNNCC 2 cut(s) 168, 494
NspV TTCGAA 1 cut(s) 504
PctI GAATGC 1 cut(s) 500
PdmI GAANNNNTTC 1 cut(s) 320
PflMI CCANNNNNTGG 1 cut(s) 294
PkrI GCNGC 2 cut(s) 138, 405
Ple19I CGATCG 1 cut(s) 477
PleI GAGTC 1 cut(s) 211
PpsI GAGTC 1 cut(s) 211
Psp6I CCWGG 1 cut(s) 236
PspGI CCWGG 1 cut(s) 236
PspN4I GGNNCC 2 cut(s) 168, 494
PspPI GGNCC 2 cut(s) 28, 567
PsuI RGATCY 1 cut(s) 109
PvuI CGATCG 1 cut(s) 477
RsaI GTAC 1 cut(s) 47
RsaNI GTAC 1 cut(s) 46
RseI CAYNNNNRTG 1 cut(s) 56
SalI GTCGAC 1 cut(s) 299
SaqAI TTAA 2 cut(s) 398, 561
SatI GCNGC 2 cut(s) 137, 404
Sau3AI GATC 2 cut(s) 109, 474
Sau96I GGNCC 2 cut(s) 28, 567
SchI GAGTC 1 cut(s) 212
ScrFI CCNGG 2 cut(s) 152, 238
SduI GDGCHC 2 cut(s) 171, 283
SfaNI GCATC 3 cut(s) 93, 219, 316
SfuI TTCGAA 1 cut(s) 504
SinI GGWCC 2 cut(s) 28, 567
SmiMI CAYNNNNRTG 1 cut(s) 56
Sse9I AATT 2 cut(s) 509, 623
SspMI CTAG 2 cut(s) 39, 407
StyD4I CCNGG 2 cut(s) 150, 236
StyI CCWWGG 2 cut(s) 69, 162
TaaI ACNGT 1 cut(s) 586
TaiI ACGT 1 cut(s) 309
TaqI TCGA 3 cut(s) 300, 324, 504
TasI AATT 2 cut(s) 509, 623
Tru1I TTAA 2 cut(s) 398, 561
Tru9I TTAA 2 cut(s) 398, 561
TseI GCWGC 2 cut(s) 136, 403
TspDTI ATGAA 3 cut(s) 108, 376, 636
TspGWI ACGGA 1 cut(s) 23
Van91I CCANNNNNTGG 1 cut(s) 294
VpaK11BI GGWCC 2 cut(s) 28, 567
XapI RAATTY 2 cut(s) 509, 623
XmiI GTMKAC 1 cut(s) 300
XmnI GAANNNNTTC 1 cut(s) 320
XspI CTAG 2 cut(s) 39, 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.