RchiOBHm_Chr5g0067311

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0015584
Physical Location & Seq
Forward (+)
73514037 .. 73515013
977 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34303

Sequence Viewer

Length: 663 bp
ATGTCGAAGGCAGAGGTTATTCTTTTGGACTGTTGGATTAGCCCTTTCTGCATGAGGGTGAAAATTGCTTTGGAAGAAAAGGGTGTTGCATACGAAAGCCAGGCTGAGGATTTGTTTGGAGGCAAGAGTGAACTTCTACTTACATCAAACCCCCATGGCAAGGTGCCTGTGTTGCTCCACAATGGGAAACCTGTGTCCGAGTCCGCCATCATAGTTGCCTACATCGATGAATCCTGGTCTTCCTCACCATTGCTCCCACCTTGTGCCTACGGTCGAGCTCAAGCAAGGTTTTGGGCTGACTACATTGACAAAAAGGTGTTTGATGCGGGCAAAGCCATCTTCATGAGCAAAGGAGAAGCAGTAGAGGTTGGTAAGAAGGACTTCATTGAGATTATAAAGACTCTAGAGAAGGCTTTGGGAGATAAGGATTTCTTTAATGGTGATACCTTCGGTTTCGTCGACATCATAGGCATTGCCATGACGAGTTGGTTTCCTGCTTATGAGAAGTTCGGGAGCTTCAAGCTTGAAGATCATTGCCCCAAATTCTCAGCTTGGATCAAGAGGAGCTGGCAGAGGGAGAGTGTAGCCAAGGTCATTCCAGAGGCAGAGAAGGTCATTGAATTTGTGACCATGTTTAGGAAGATGATGGGAGCCGAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418
Pfam Domains
Protein Families
GST_C GST_C_2 GST_N

Protein Analysis

220

Amino Acids

24.71

Weight (kDa)

5.56

Isoelectric Point (pI)

37.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 5 - 77 4.7e-16 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 10 - 78 2.8e-15 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 14 - 77 4.6e-14 Glutathione S-transferase, N-terminal domain
GST_C_2 PF13410 120 - 188 2.9e-06 Glutathione S-transferase, C-terminal domain
GST_C PF00043 121 - 192 6.6e-06 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015584)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g37420
malus_domestica MD09G1279700.v1.1 MD17G1271700.v1.1 MD17G1286500.v1.1
prunus_persica Prupe.3G014500_v2.0.a1
pyrus_communis pycom17g26990
rosa_chinensis RchiOBHm_Chr5g0067311
rosa_laevigata RLG00000035926
rosa_multiflora Rmu_sc0000015.1_g000002
rosa_roxburghii Rroxscaffold_1G00013760
rosa_rugosa Rorug05G0384100
rosa_samantha Rh5AG443500 Rh5BG460500 Rh5CG482800 Rh5DG475600
rosa_wichuraiana Rw5G041420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 395
AccB1I GGYRCC 1 cut(s) 163
AccI GTMKAC 1 cut(s) 459
AciI CCGC 2 cut(s) 204, 326
AclWI GGATC 1 cut(s) 563
AcsI RAATTY 2 cut(s) 542, 620
AdeI CACNNNGTG 1 cut(s) 263
AfiI CCNNNNNNNGG 3 cut(s) 106, 160, 636
AgsI TTSAA 3 cut(s) 520, 527, 620
AjnI CCWGG 2 cut(s) 99, 233
AjuI GAANNNNNNNTTGG 2 cut(s) 53, 85
AluBI AGCT 5 cut(s) 278, 516, 523, 551, 567
AluI AGCT 5 cut(s) 278, 516, 523, 551, 567
Alw21I GWGCWC 1 cut(s) 280
AlwI GGATC 1 cut(s) 563
ApoI RAATTY 2 cut(s) 542, 620
Asp700I GAANNNNTTC 1 cut(s) 380
AsuHPI GGTGA 3 cut(s) 70, 237, 452
BanI GGYRCC 1 cut(s) 163
BanII GRGCYC 1 cut(s) 280
BbsI GAAGAC 1 cut(s) 231
Bbv12I GWGCWC 1 cut(s) 280
BbvCI CCTCAGC 1 cut(s) 105
BccI CCATC 3 cut(s) 215, 344, 640
BciT130I CCWGG 2 cut(s) 101, 235
BfaI CTAG 1 cut(s) 404
Bme1390I CCNGG 2 cut(s) 101, 235
BmiI GGNNCC 2 cut(s) 165, 652
BmrFI CCNGG 2 cut(s) 101, 235
BmsI GCATC 1 cut(s) 313
BpiI GAAGAC 1 cut(s) 231
Bpu10I CCTNAGC 1 cut(s) 105
BpuEI CTTGAG 1 cut(s) 264
Bsa29I ATCGAT 1 cut(s) 225
BsaJI CCNNGG 2 cut(s) 154, 588
BsaXI ACNNNNNCTCC 4 cut(s) 237, 267, 345, 375
Bsc4I CCNNNNNNNGG 3 cut(s) 106, 160, 636
Bse3DI GCAATG 3 cut(s) 248, 471, 532
BseBI CCWGG 2 cut(s) 101, 235
BseCI ATCGAT 1 cut(s) 225
BseDI CCNNGG 2 cut(s) 154, 588
BseLI CCNNNNNNNGG 3 cut(s) 106, 160, 636
BseMI GCAATG 3 cut(s) 248, 471, 532
BseMII CTCAG 2 cut(s) 96, 561
BseRI GAGGAG 1 cut(s) 577
Bsh1285I CGRYCG 1 cut(s) 274
BshNI GGYRCC 1 cut(s) 163
BshVI ATCGAT 1 cut(s) 225
BsiEI CGRYCG 1 cut(s) 274
BsiHKAI GWGCWC 1 cut(s) 280
BslI CCNNNNNNNGG 3 cut(s) 106, 160, 636
Bsp1286I GDGCHC 1 cut(s) 280
Bsp143I GATC 2 cut(s) 529, 555
Bsp19I CCATGG 1 cut(s) 154
BspACI CCGC 2 cut(s) 204, 326
BspCNI CTCAG 2 cut(s) 97, 560
BspDI ATCGAT 1 cut(s) 225
BspHI TCATGA 1 cut(s) 342
BspLI GGNNCC 2 cut(s) 165, 652
BspPI GGATC 1 cut(s) 563
BspT107I GGYRCC 1 cut(s) 163
BsrDI GCAATG 3 cut(s) 248, 471, 532
BssECI CCNNGG 2 cut(s) 154, 588
BssMI GATC 2 cut(s) 529, 555
BssT1I CCWWGG 2 cut(s) 154, 588
Bst2UI CCWGG 2 cut(s) 101, 235
Bst4CI ACNGT 2 cut(s) 32, 272
BstC8I GCNNGC 2 cut(s) 328, 569
BstDEI CTNAG 2 cut(s) 105, 547
BstDSI CCRYGG 1 cut(s) 154
BstKTI GATC 2 cut(s) 532, 558
BstMBI GATC 2 cut(s) 529, 555
BstMCI CGRYCG 1 cut(s) 274
BstMWI GCNNNNNNNGC 3 cut(s) 48, 172, 332
BstNI CCWGG 2 cut(s) 101, 235
BstSCI CCNGG 2 cut(s) 99, 233
BstV2I GAAGAC 1 cut(s) 231
Bsu15I ATCGAT 1 cut(s) 225
BsuTUI ATCGAT 1 cut(s) 225
BtgI CCRYGG 1 cut(s) 154
Cac8I GCNNGC 2 cut(s) 328, 569
CciI TCATGA 1 cut(s) 342
ClaI ATCGAT 1 cut(s) 225
CviAII CATG 5 cut(s) 52, 155, 343, 478, 631
DdeI CTNAG 2 cut(s) 105, 547
DpnI GATC 2 cut(s) 531, 557
DpnII GATC 2 cut(s) 529, 555
DraIII CACNNNGTG 1 cut(s) 263
EciI GGCGGA 1 cut(s) 193
Ecl136II GAGCTC 1 cut(s) 278
Eco130I CCWWGG 2 cut(s) 154, 588
Eco24I GRGCYC 1 cut(s) 280
Eco53kI GAGCTC 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 278
EcoRII CCWGG 2 cut(s) 99, 233
EcoT14I CCWWGG 2 cut(s) 154, 588
EcoT38I GRGCYC 1 cut(s) 280
ErhI CCWWGG 2 cut(s) 154, 588
FaeI CATG 5 cut(s) 55, 158, 346, 481, 634
FalI AAGNNNNNCTT 4 cut(s) 365, 397, 416, 448
FatI CATG 5 cut(s) 51, 154, 342, 477, 630
FauI CCCGC 1 cut(s) 319
FblI GTMKAC 1 cut(s) 459
FriOI GRGCYC 1 cut(s) 280
FspBI CTAG 1 cut(s) 404
Hin1II CATG 5 cut(s) 55, 158, 346, 481, 634
HincII GTYRAC 1 cut(s) 460
HindII GTYRAC 1 cut(s) 460
HindIII AAGCTT 1 cut(s) 521
HinfI GANTC 3 cut(s) 200, 230, 400
HphI GGTGA 3 cut(s) 70, 237, 452
Hpy166II GTNNAC 2 cut(s) 131, 460
Hpy188I TCNGA 1 cut(s) 199
Hpy188III TCNNGA 5 cut(s) 343, 404, 511, 559, 599
Hpy8I GTNNAC 2 cut(s) 131, 460
Hpy99I CGWCG 1 cut(s) 461
HpyAV CCTTC 4 cut(s) 370, 403, 457, 604
HpyCH4III ACNGT 2 cut(s) 32, 272
HpyCH4V TGCA 2 cut(s) 51, 89
HpyF10VI GCNNNNNNNGC 3 cut(s) 48, 172, 332
HpyF3I CTNAG 2 cut(s) 105, 547
Hsp92II CATG 5 cut(s) 55, 158, 346, 481, 634
Kzo9I GATC 2 cut(s) 529, 555
LmnI GCTCC 5 cut(s) 180, 258, 513, 564, 650
LpnPI CCDG 9 cut(s) 86, 113, 180, 204, 220, 247, 507, 553, 612
LweI GCATC 1 cut(s) 313
MaeI CTAG 1 cut(s) 404
MaeIII GTNAC 1 cut(s) 625
MalI GATC 2 cut(s) 531, 557
MboI GATC 2 cut(s) 529, 555
MboII GAAGA 5 cut(s) 86, 231, 331, 539, 652
MhlI GDGCHC 1 cut(s) 280
MluCI AATT 3 cut(s) 63, 542, 620
MlyI GAGTC 2 cut(s) 209, 394
MmeI TCCRAC 1 cut(s) 14
MnlI CCTC 9 cut(s) 7, 48, 100, 113, 253, 358, 555, 567, 595
MroXI GAANNNNTTC 1 cut(s) 380
MseI TTAA 1 cut(s) 435
MslI CAYNNNNRTG 3 cut(s) 56, 341, 476
MspR9I CCNGG 2 cut(s) 101, 235
MvaI CCWGG 2 cut(s) 101, 235
MwoI GCNNNNNNNGC 3 cut(s) 48, 172, 332
NcoI CCATGG 1 cut(s) 154
NdeII GATC 2 cut(s) 529, 555
NlaIII CATG 5 cut(s) 55, 158, 346, 481, 634
NlaIV GGNNCC 2 cut(s) 165, 652
NmuCI GTSAC 1 cut(s) 625
PagI TCATGA 1 cut(s) 342
PcsI WCGNNNNNNNCGW 1 cut(s) 456
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 1 cut(s) 230
PleI GAGTC 2 cut(s) 208, 394
PpsI GAGTC 2 cut(s) 208, 394
PsiI TTATAA 1 cut(s) 395
Psp124BI GAGCTC 1 cut(s) 280
Psp6I CCWGG 2 cut(s) 99, 233
PspGI CCWGG 2 cut(s) 99, 233
PspN4I GGNNCC 2 cut(s) 165, 652
RseI CAYNNNNRTG 3 cut(s) 56, 341, 476
SacI GAGCTC 1 cut(s) 280
SalI GTCGAC 1 cut(s) 458
SaqAI TTAA 1 cut(s) 435
Sau3AI GATC 2 cut(s) 529, 555
SchI GAGTC 2 cut(s) 209, 394
ScrFI CCNGG 2 cut(s) 101, 235
SduI GDGCHC 1 cut(s) 280
SfaNI GCATC 1 cut(s) 313
SmiMI CAYNNNNRTG 3 cut(s) 56, 341, 476
SmlI CTYRAG 1 cut(s) 279
SmoI CTYRAG 1 cut(s) 279
Sse9I AATT 3 cut(s) 63, 542, 620
SsiI CCGC 2 cut(s) 204, 326
SspMI CTAG 1 cut(s) 404
SstI GAGCTC 1 cut(s) 280
StyD4I CCNGG 2 cut(s) 99, 233
StyI CCWWGG 2 cut(s) 154, 588
TaaI ACNGT 2 cut(s) 32, 272
TaqI TCGA 4 cut(s) 5, 225, 274, 459
TasI AATT 3 cut(s) 63, 542, 620
TfiI GAWTC 1 cut(s) 230
Tru1I TTAA 1 cut(s) 435
Tru9I TTAA 1 cut(s) 435
TseFI GTSAC 1 cut(s) 625
Tsp45I GTSAC 1 cut(s) 625
TspDTI ATGAA 3 cut(s) 243, 331, 373
XapI RAATTY 2 cut(s) 542, 620
XbaI TCTAGA 1 cut(s) 403
XmiI GTMKAC 1 cut(s) 459
XmnI GAANNNNTTC 1 cut(s) 380
XspI CTAG 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.