Rh5AG443500

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0015584
Physical Location & Seq
Forward (+)
76111471 .. 76112396
926 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG443500.1

Sequence Viewer

Length: 612 bp
ATGAGGGTGAAAATTGCTTTGGAAGAAAAGGGTGTTGCATACGAAAGCCAGGCTGAGGATTTGTTTGGAGGCAAGAGTGAACTTCTACTTACATCAAACCCCCATGGCAAGGTGCCTGTGTTGCTCCACAATGGGAAACCTGTGTCCGAGTCCGCCATCATAGTTGCCTACATCGATGAATCCTGGTCTTCCTCACCATTGCTCCCACCTTGTGCCTACGGTCGAGCTCAAGCAAGGTTTTGGGCTGACTACATTGACAAAAAGGTGTTTGATGCGGGCAAAGCCATCTTCATGAGCAAAGGAGAAGCAGTAGAGGTTGGTAAGAAGGACTTCATTGAGATTATAAAGACTCTAGAGAAGGCTTTGGGAGATAAGGATTTCTTTAATGGTGATACCTTCGGTTTCGTCGACATCATAGGCATTGCCATGACGAGTTGGTTTCCTGCTTATGAGAAGTTCGGGAGCTTCAAGCTTGAAGATCATTGCCCCAAATTCTCAGCTTGGATCAAGAGGAGCTGGCAGAGGGAGAGTGTAGCCAAGGTCATTCCAGAGGCAGAGAAGGTCATTGAATTTGTGACCATGTTTAGGAAGATGATGGGAGCCGAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418
Pfam Domains
Protein Families
GST_C GST_C_2 GST_N

Protein Analysis

203

Amino Acids

22.77

Weight (kDa)

5.75

Isoelectric Point (pI)

30.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 1 - 60 8.7e-14 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 1 - 61 3.7e-11 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 2 - 60 1.7e-10 Glutathione S-transferase, N-terminal domain
GST_C_2 PF13410 103 - 171 2.5e-06 Glutathione S-transferase, C-terminal domain
GST_C PF00043 104 - 175 5.5e-06 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015584)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g37420
malus_domestica MD09G1279700.v1.1 MD17G1271700.v1.1 MD17G1286500.v1.1
prunus_persica Prupe.3G014500_v2.0.a1
pyrus_communis pycom17g26990
rosa_chinensis RchiOBHm_Chr5g0067311
rosa_laevigata RLG00000035926
rosa_multiflora Rmu_sc0000015.1_g000002
rosa_roxburghii Rroxscaffold_1G00013760
rosa_rugosa Rorug05G0384100
rosa_samantha Rh5AG443500 Rh5BG460500 Rh5CG482800 Rh5DG475600
rosa_wichuraiana Rw5G041420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 344
AccB1I GGYRCC 1 cut(s) 112
AccI GTMKAC 1 cut(s) 408
AciI CCGC 2 cut(s) 153, 275
AclWI GGATC 1 cut(s) 512
AcsI RAATTY 2 cut(s) 491, 569
AdeI CACNNNGTG 1 cut(s) 212
AfiI CCNNNNNNNGG 3 cut(s) 55, 109, 585
AgsI TTSAA 3 cut(s) 469, 476, 569
AjnI CCWGG 2 cut(s) 48, 182
AjuI GAANNNNNNNTTGG 1 cut(s) 34
AluBI AGCT 5 cut(s) 227, 465, 472, 500, 516
AluI AGCT 5 cut(s) 227, 465, 472, 500, 516
Alw21I GWGCWC 1 cut(s) 229
AlwI GGATC 1 cut(s) 512
ApoI RAATTY 2 cut(s) 491, 569
Asp700I GAANNNNTTC 1 cut(s) 329
AsuHPI GGTGA 3 cut(s) 19, 186, 401
BanI GGYRCC 1 cut(s) 112
BanII GRGCYC 1 cut(s) 229
BbsI GAAGAC 1 cut(s) 180
Bbv12I GWGCWC 1 cut(s) 229
BbvCI CCTCAGC 1 cut(s) 54
BccI CCATC 3 cut(s) 164, 293, 589
BciT130I CCWGG 2 cut(s) 50, 184
BfaI CTAG 1 cut(s) 353
Bme1390I CCNGG 2 cut(s) 50, 184
BmiI GGNNCC 2 cut(s) 114, 601
BmrFI CCNGG 2 cut(s) 50, 184
BmsI GCATC 1 cut(s) 262
BpiI GAAGAC 1 cut(s) 180
Bpu10I CCTNAGC 1 cut(s) 54
BpuEI CTTGAG 1 cut(s) 213
Bsa29I ATCGAT 1 cut(s) 174
BsaJI CCNNGG 2 cut(s) 103, 537
BsaXI ACNNNNNCTCC 4 cut(s) 186, 216, 294, 324
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 109, 585
Bse3DI GCAATG 3 cut(s) 197, 420, 481
BseBI CCWGG 2 cut(s) 50, 184
BseCI ATCGAT 1 cut(s) 174
BseDI CCNNGG 2 cut(s) 103, 537
BseLI CCNNNNNNNGG 3 cut(s) 55, 109, 585
BseMI GCAATG 3 cut(s) 197, 420, 481
BseMII CTCAG 2 cut(s) 45, 510
BseRI GAGGAG 1 cut(s) 526
Bsh1285I CGRYCG 1 cut(s) 223
BshNI GGYRCC 1 cut(s) 112
BshVI ATCGAT 1 cut(s) 174
BsiEI CGRYCG 1 cut(s) 223
BsiHKAI GWGCWC 1 cut(s) 229
BslI CCNNNNNNNGG 3 cut(s) 55, 109, 585
Bsp1286I GDGCHC 1 cut(s) 229
Bsp143I GATC 2 cut(s) 478, 504
Bsp19I CCATGG 1 cut(s) 103
BspACI CCGC 2 cut(s) 153, 275
BspCNI CTCAG 2 cut(s) 46, 509
BspDI ATCGAT 1 cut(s) 174
BspHI TCATGA 1 cut(s) 291
BspLI GGNNCC 2 cut(s) 114, 601
BspPI GGATC 1 cut(s) 512
BspT107I GGYRCC 1 cut(s) 112
BsrDI GCAATG 3 cut(s) 197, 420, 481
BssECI CCNNGG 2 cut(s) 103, 537
BssMI GATC 2 cut(s) 478, 504
BssT1I CCWWGG 2 cut(s) 103, 537
Bst2UI CCWGG 2 cut(s) 50, 184
Bst4CI ACNGT 1 cut(s) 221
BstC8I GCNNGC 2 cut(s) 277, 518
BstDEI CTNAG 2 cut(s) 54, 496
BstDSI CCRYGG 1 cut(s) 103
BstKTI GATC 2 cut(s) 481, 507
BstMBI GATC 2 cut(s) 478, 504
BstMCI CGRYCG 1 cut(s) 223
BstMWI GCNNNNNNNGC 2 cut(s) 121, 281
BstNI CCWGG 2 cut(s) 50, 184
BstSCI CCNGG 2 cut(s) 48, 182
BstV2I GAAGAC 1 cut(s) 180
Bsu15I ATCGAT 1 cut(s) 174
BsuTUI ATCGAT 1 cut(s) 174
BtgI CCRYGG 1 cut(s) 103
Cac8I GCNNGC 2 cut(s) 277, 518
CciI TCATGA 1 cut(s) 291
ClaI ATCGAT 1 cut(s) 174
CviAII CATG 4 cut(s) 104, 292, 427, 580
DdeI CTNAG 2 cut(s) 54, 496
DpnI GATC 2 cut(s) 480, 506
DpnII GATC 2 cut(s) 478, 504
DraIII CACNNNGTG 1 cut(s) 212
EciI GGCGGA 1 cut(s) 142
Ecl136II GAGCTC 1 cut(s) 227
Eco130I CCWWGG 2 cut(s) 103, 537
Eco24I GRGCYC 1 cut(s) 229
Eco53kI GAGCTC 1 cut(s) 227
EcoICRI GAGCTC 1 cut(s) 227
EcoRII CCWGG 2 cut(s) 48, 182
EcoT14I CCWWGG 2 cut(s) 103, 537
EcoT38I GRGCYC 1 cut(s) 229
ErhI CCWWGG 2 cut(s) 103, 537
FaeI CATG 4 cut(s) 107, 295, 430, 583
FaiI YATR 9 cut(s) 40, 105, 161, 293, 344, 416, 428, 450, 581
FalI AAGNNNNNCTT 4 cut(s) 314, 346, 365, 397
FatI CATG 4 cut(s) 103, 291, 426, 579
FauI CCCGC 1 cut(s) 268
FblI GTMKAC 1 cut(s) 408
FriOI GRGCYC 1 cut(s) 229
FspBI CTAG 1 cut(s) 353
Hin1II CATG 4 cut(s) 107, 295, 430, 583
HincII GTYRAC 1 cut(s) 409
HindII GTYRAC 1 cut(s) 409
HindIII AAGCTT 1 cut(s) 470
HinfI GANTC 3 cut(s) 149, 179, 349
HphI GGTGA 3 cut(s) 19, 186, 401
Hpy166II GTNNAC 2 cut(s) 80, 409
Hpy188I TCNGA 1 cut(s) 148
Hpy188III TCNNGA 5 cut(s) 292, 353, 460, 508, 548
Hpy8I GTNNAC 2 cut(s) 80, 409
Hpy99I CGWCG 1 cut(s) 410
HpyAV CCTTC 4 cut(s) 319, 352, 406, 553
HpyCH4III ACNGT 1 cut(s) 221
HpyCH4V TGCA 1 cut(s) 38
HpyF10VI GCNNNNNNNGC 2 cut(s) 121, 281
HpyF3I CTNAG 2 cut(s) 54, 496
Hsp92II CATG 4 cut(s) 107, 295, 430, 583
Kzo9I GATC 2 cut(s) 478, 504
LmnI GCTCC 5 cut(s) 129, 207, 462, 513, 599
LpnPI CCDG 9 cut(s) 35, 62, 129, 153, 169, 196, 456, 502, 561
LweI GCATC 1 cut(s) 262
MaeI CTAG 1 cut(s) 353
MaeIII GTNAC 1 cut(s) 574
MalI GATC 2 cut(s) 480, 506
MboI GATC 2 cut(s) 478, 504
MboII GAAGA 5 cut(s) 35, 180, 280, 488, 601
MhlI GDGCHC 1 cut(s) 229
MluCI AATT 3 cut(s) 12, 491, 569
MlyI GAGTC 2 cut(s) 158, 343
MnlI CCTC 7 cut(s) 49, 62, 202, 307, 504, 516, 544
MroXI GAANNNNTTC 1 cut(s) 329
MseI TTAA 1 cut(s) 384
MslI CAYNNNNRTG 2 cut(s) 290, 425
MspR9I CCNGG 2 cut(s) 50, 184
MvaI CCWGG 2 cut(s) 50, 184
MwoI GCNNNNNNNGC 2 cut(s) 121, 281
NcoI CCATGG 1 cut(s) 103
NdeII GATC 2 cut(s) 478, 504
NlaIII CATG 4 cut(s) 107, 295, 430, 583
NlaIV GGNNCC 2 cut(s) 114, 601
NmuCI GTSAC 1 cut(s) 574
PagI TCATGA 1 cut(s) 291
PcsI WCGNNNNNNNCGW 1 cut(s) 405
PdmI GAANNNNTTC 1 cut(s) 329
PfeI GAWTC 1 cut(s) 179
PleI GAGTC 2 cut(s) 157, 343
PpsI GAGTC 2 cut(s) 157, 343
PsiI TTATAA 1 cut(s) 344
Psp124BI GAGCTC 1 cut(s) 229
Psp6I CCWGG 2 cut(s) 48, 182
PspGI CCWGG 2 cut(s) 48, 182
PspN4I GGNNCC 2 cut(s) 114, 601
RseI CAYNNNNRTG 2 cut(s) 290, 425
SacI GAGCTC 1 cut(s) 229
SalI GTCGAC 1 cut(s) 407
SaqAI TTAA 1 cut(s) 384
Sau3AI GATC 2 cut(s) 478, 504
SchI GAGTC 2 cut(s) 158, 343
ScrFI CCNGG 2 cut(s) 50, 184
SduI GDGCHC 1 cut(s) 229
SfaNI GCATC 1 cut(s) 262
SmiMI CAYNNNNRTG 2 cut(s) 290, 425
SmlI CTYRAG 1 cut(s) 228
SmoI CTYRAG 1 cut(s) 228
Sse9I AATT 3 cut(s) 12, 491, 569
SsiI CCGC 2 cut(s) 153, 275
SspMI CTAG 1 cut(s) 353
SstI GAGCTC 1 cut(s) 229
StyD4I CCNGG 2 cut(s) 48, 182
StyI CCWWGG 2 cut(s) 103, 537
TaaI ACNGT 1 cut(s) 221
TaqI TCGA 3 cut(s) 174, 223, 408
TasI AATT 3 cut(s) 12, 491, 569
TfiI GAWTC 1 cut(s) 179
Tru1I TTAA 1 cut(s) 384
Tru9I TTAA 1 cut(s) 384
TseFI GTSAC 1 cut(s) 574
Tsp45I GTSAC 1 cut(s) 574
TspDTI ATGAA 3 cut(s) 192, 280, 322
XapI RAATTY 2 cut(s) 491, 569
XbaI TCTAGA 1 cut(s) 352
XmiI GTMKAC 1 cut(s) 408
XmnI GAANNNNTTC 1 cut(s) 329
XspI CTAG 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.