MD17G1286500.v1.1

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0015584
Physical Location & Seq
Forward (+)
34609141 .. 34610079
939 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1286500.v1.1.491

Sequence Viewer

Length: 531 bp
ATGGCGACGGAAGATGTTGTTCTCTTGGACCTCTGGGCTAGCCCGTACTGCATGAGGGTGAAGATTGCCTTGGAGGAAAAGGGCATCAAATATGAAGAGAGAGCTGAGGATCTCTTTGGAGGCAAGAGTGAACTGCTGCTCACATCCAACCCGGTTTACGCCAAGGTGCCCGTGCTTCTTCACAAGGGAAAACCATTGTGTGAGTCAAGCATCATCGTCGGCTACATTGATGAGACCTGGGCTTCCCCACCATTGCTCCCATCTTGCCCTTATGCTCGTGCTCAGGCCAAGTTCTGGGTCGACTACGTTGACAAGAAGCTGTTCGATGCAGGAGGCAATATATGGAGAACCAAAGGAGAAGCACAAGAGGTGGCTGTAAAAGACTTCATAGAAATCTTAAAGCAGCTAGAGAAAGTTCTTGGGGACAAAGATTTCTTCGGTGGCGATAGCTTTGGTTTCGTTGACATCATAACGATCGCTATAACAAGCTGGTTCCTTGCATTCGAAAAATTTTGGAAACTTCAAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418
Pfam Domains
Protein Families
GST_C GST_C_2 GST_N

Protein Analysis

177

Amino Acids

19.88

Weight (kDa)

5.03

Isoelectric Point (pI)

39.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 6 - 78 1.7e-16 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 11 - 81 1.4e-14 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 13 - 79 3.9e-13 Glutathione S-transferase, N-terminal domain
GST_C PF00043 120 - 169 6.4e-06 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015584)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g37420
malus_domestica MD09G1279700.v1.1 MD17G1271700.v1.1 MD17G1286500.v1.1
prunus_persica Prupe.3G014500_v2.0.a1
pyrus_communis pycom17g26990
rosa_chinensis RchiOBHm_Chr5g0067311
rosa_laevigata RLG00000035926
rosa_multiflora Rmu_sc0000015.1_g000002
rosa_roxburghii Rroxscaffold_1G00013760
rosa_rugosa Rorug05G0384100
rosa_samantha Rh5AG443500 Rh5BG460500 Rh5CG482800 Rh5DG475600
rosa_wichuraiana Rw5G041420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 166
AccB7I CCANNNNNTGG 1 cut(s) 294
AccI GTMKAC 1 cut(s) 300
AclWI GGATC 1 cut(s) 117
AcsI RAATTY 1 cut(s) 509
AfaI GTAC 1 cut(s) 47
AfiI CCNNNNNNNGG 1 cut(s) 294
AgsI TTSAA 1 cut(s) 524
AjnI CCWGG 1 cut(s) 236
AjuI GAANNNNNNNTTGG 2 cut(s) 53, 85
AluBI AGCT 5 cut(s) 104, 319, 406, 450, 489
AluI AGCT 5 cut(s) 104, 319, 406, 450, 489
Alw21I GWGCWC 1 cut(s) 283
Alw26I GTCTC 1 cut(s) 227
AlwI GGATC 1 cut(s) 117
AoxI GGCC 1 cut(s) 285
ApeKI GCWGC 2 cut(s) 136, 403
ApoI RAATTY 1 cut(s) 509
Asp700I GAANNNNTTC 1 cut(s) 320
AspS9I GGNCC 1 cut(s) 28
AsuC2I CCSGG 1 cut(s) 152
AsuHPI GGTGA 1 cut(s) 70
AsuII TTCGAA 1 cut(s) 504
AsuNHI GCTAGC 1 cut(s) 38
AvaII GGWCC 1 cut(s) 28
BaeGI GKGCMC 1 cut(s) 171
BanI GGYRCC 1 cut(s) 166
BauI CACGAG 1 cut(s) 276
Bbv12I GWGCWC 1 cut(s) 283
BbvCI CCTCAGC 1 cut(s) 105
BbvI GCAGC 2 cut(s) 123, 415
BccI CCATC 1 cut(s) 268
BcgI CGANNNNNNTGC 2 cut(s) 199, 233
BciT130I CCWGG 1 cut(s) 238
BcnI CCSGG 1 cut(s) 152
BcoDI GTCTC 1 cut(s) 227
BfaI CTAG 2 cut(s) 39, 407
BisI GCNGC 2 cut(s) 137, 404
BlsI GCNGC 2 cut(s) 138, 405
Bme1390I CCNGG 2 cut(s) 152, 238
Bme18I GGWCC 1 cut(s) 28
BmgT120I GGNCC 1 cut(s) 28
BmiI GGNNCC 2 cut(s) 168, 494
BmrFI CCNGG 2 cut(s) 152, 238
BmsI GCATC 3 cut(s) 93, 219, 316
BmtI GCTAGC 1 cut(s) 42
Bpu10I CCTNAGC 2 cut(s) 105, 282
Bpu14I TTCGAA 1 cut(s) 504
BpuMI CCSGG 1 cut(s) 152
BsaI GGTCTC 1 cut(s) 227
BsaJI CCNNGG 3 cut(s) 69, 162, 237
BsaXI ACNNNNNCTCC 2 cut(s) 240, 270
Bsc4I CCNNNNNNNGG 1 cut(s) 294
Bse3DI GCAATG 1 cut(s) 251
BseBI CCWGG 1 cut(s) 238
BseDI CCNNGG 3 cut(s) 69, 162, 237
BseGI GGATG 1 cut(s) 143
BseLI CCNNNNNNNGG 1 cut(s) 294
BseMI GCAATG 1 cut(s) 251
BseMII CTCAG 2 cut(s) 96, 296
BseSI GKGCMC 1 cut(s) 171
BseXI GCAGC 2 cut(s) 123, 415
Bsh1285I CGRYCG 1 cut(s) 477
BshFI GGCC 1 cut(s) 287
BshNI GGYRCC 1 cut(s) 166
BsiEI CGRYCG 1 cut(s) 477
BsiHKAI GWGCWC 1 cut(s) 283
BsiSI CCGG 1 cut(s) 152
BslFI GGGAC 1 cut(s) 437
BslI CCNNNNNNNGG 1 cut(s) 294
BsmAI GTCTC 1 cut(s) 227
BsmFI GGGAC 1 cut(s) 437
BsmI GAATGC 1 cut(s) 500
BsnI GGCC 1 cut(s) 287
Bso31I GGTCTC 1 cut(s) 227
Bsp119I TTCGAA 1 cut(s) 504
Bsp1286I GDGCHC 2 cut(s) 171, 283
Bsp143I GATC 2 cut(s) 109, 474
BspANI GGCC 1 cut(s) 287
BspCNI CTCAG 2 cut(s) 97, 295
BspLI GGNNCC 2 cut(s) 168, 494
BspOI GCTAGC 1 cut(s) 42
BspPI GGATC 1 cut(s) 117
BspT104I TTCGAA 1 cut(s) 504
BspT107I GGYRCC 1 cut(s) 166
BspTNI GGTCTC 1 cut(s) 227
BsrDI GCAATG 1 cut(s) 251
BssECI CCNNGG 3 cut(s) 69, 162, 237
BssMI GATC 2 cut(s) 109, 474
BssSI CACGAG 1 cut(s) 276
BssT1I CCWWGG 2 cut(s) 69, 162
Bst2BI CACGAG 1 cut(s) 276
Bst2UI CCWGG 1 cut(s) 238
Bst6I CTCTTC 1 cut(s) 90
BstBI TTCGAA 1 cut(s) 504
BstC8I GCNNGC 1 cut(s) 40
BstDEI CTNAG 2 cut(s) 105, 282
BstF5I GGATG 1 cut(s) 143
BstKTI GATC 2 cut(s) 112, 477
BstMAI GTCTC 1 cut(s) 227
BstMBI GATC 2 cut(s) 109, 474
BstMCI CGRYCG 1 cut(s) 477
BstMWI GCNNNNNNNGC 1 cut(s) 48
BstNI CCWGG 1 cut(s) 238
BstSCI CCNGG 2 cut(s) 150, 236
BstSLI GKGCMC 1 cut(s) 171
BstV1I GCAGC 2 cut(s) 123, 415
BstX2I RGATCY 1 cut(s) 109
BstYI RGATCY 1 cut(s) 109
BsuRI GGCC 1 cut(s) 287
BtsCI GGATG 1 cut(s) 143
Cac8I GCNNGC 1 cut(s) 40
Cfr13I GGNCC 1 cut(s) 28
Csp6I GTAC 1 cut(s) 46
CviAII CATG 1 cut(s) 52
CviQI GTAC 1 cut(s) 46
DdeI CTNAG 2 cut(s) 105, 282
DpnI GATC 2 cut(s) 111, 476
DpnII GATC 2 cut(s) 109, 474
Eam1104I CTCTTC 1 cut(s) 90
EarI CTCTTC 1 cut(s) 90
Eco130I CCWWGG 2 cut(s) 69, 162
Eco31I GGTCTC 1 cut(s) 227
Eco47I GGWCC 1 cut(s) 28
EcoRII CCWGG 1 cut(s) 236
EcoT14I CCWWGG 2 cut(s) 69, 162
ErhI CCWWGG 2 cut(s) 69, 162
FaeI CATG 1 cut(s) 55
FaiI YATR 8 cut(s) 53, 93, 273, 341, 343, 389, 470, 482
FalI AAGNNNNNCTT 2 cut(s) 53, 85
FaqI GGGAC 1 cut(s) 437
FatI CATG 1 cut(s) 51
FblI GTMKAC 1 cut(s) 300
Fnu4HI GCNGC 2 cut(s) 137, 404
FokI GGATG 1 cut(s) 130
Fsp4HI GCNGC 2 cut(s) 137, 404
FspBI CTAG 2 cut(s) 39, 407
GluI GCNGC 2 cut(s) 137, 404
HaeIII GGCC 1 cut(s) 287
HapII CCGG 1 cut(s) 152
Hin1II CATG 1 cut(s) 55
HincII GTYRAC 3 cut(s) 301, 310, 463
HindII GTYRAC 3 cut(s) 301, 310, 463
HinfI GANTC 1 cut(s) 203
HpaII CCGG 1 cut(s) 152
HphI GGTGA 1 cut(s) 70
Hpy166II GTNNAC 5 cut(s) 131, 157, 301, 310, 463
Hpy8I GTNNAC 5 cut(s) 131, 157, 301, 310, 463
Hpy99I CGWCG 2 cut(s) 10, 221
HpyCH4IV ACGT 1 cut(s) 306
HpyCH4V TGCA 3 cut(s) 51, 329, 500
HpyF10VI GCNNNNNNNGC 1 cut(s) 48
HpyF3I CTNAG 2 cut(s) 105, 282
HpySE526I ACGT 1 cut(s) 306
Hsp92II CATG 1 cut(s) 55
Kzo9I GATC 2 cut(s) 109, 474
LmnI GCTCC 1 cut(s) 261
LpnPI CCDG 8 cut(s) 19, 165, 223, 250, 269, 280, 315, 475
Lsp1109I GCAGC 2 cut(s) 123, 415
LweI GCATC 3 cut(s) 93, 219, 316
MaeI CTAG 2 cut(s) 39, 407
MaeII ACGT 1 cut(s) 306
MalI GATC 2 cut(s) 111, 476
MboI GATC 2 cut(s) 109, 474
MboII GAAGA 5 cut(s) 23, 73, 107, 170, 427
MflI RGATCY 1 cut(s) 109
MhlI GDGCHC 2 cut(s) 171, 283
MluCI AATT 1 cut(s) 509
MlyI GAGTC 1 cut(s) 212
MmeI TCCRAC 1 cut(s) 171
MnlI CCTC 7 cut(s) 41, 48, 67, 100, 113, 326, 361
MroXI GAANNNNTTC 1 cut(s) 320
MseI TTAA 1 cut(s) 398
MslI CAYNNNNRTG 1 cut(s) 56
MspI CCGG 1 cut(s) 152
MspR9I CCNGG 2 cut(s) 152, 238
Mva1269I GAATGC 1 cut(s) 500
MvaI CCWGG 1 cut(s) 238
MwoI GCNNNNNNNGC 1 cut(s) 48
NciI CCSGG 1 cut(s) 152
NdeII GATC 2 cut(s) 109, 474
NheI GCTAGC 1 cut(s) 38
NlaIII CATG 1 cut(s) 55
NlaIV GGNNCC 2 cut(s) 168, 494
NspV TTCGAA 1 cut(s) 504
PctI GAATGC 1 cut(s) 500
PdmI GAANNNNTTC 1 cut(s) 320
PflMI CCANNNNNTGG 1 cut(s) 294
PkrI GCNGC 2 cut(s) 138, 405
Ple19I CGATCG 1 cut(s) 477
PleI GAGTC 1 cut(s) 211
PpsI GAGTC 1 cut(s) 211
Psp6I CCWGG 1 cut(s) 236
PspGI CCWGG 1 cut(s) 236
PspN4I GGNNCC 2 cut(s) 168, 494
PspPI GGNCC 1 cut(s) 28
PsuI RGATCY 1 cut(s) 109
PvuI CGATCG 1 cut(s) 477
RsaI GTAC 1 cut(s) 47
RsaNI GTAC 1 cut(s) 46
RseI CAYNNNNRTG 1 cut(s) 56
SalI GTCGAC 1 cut(s) 299
SaqAI TTAA 1 cut(s) 398
SatI GCNGC 2 cut(s) 137, 404
Sau3AI GATC 2 cut(s) 109, 474
Sau96I GGNCC 1 cut(s) 28
SchI GAGTC 1 cut(s) 212
ScrFI CCNGG 2 cut(s) 152, 238
SduI GDGCHC 2 cut(s) 171, 283
SfaNI GCATC 3 cut(s) 93, 219, 316
SfuI TTCGAA 1 cut(s) 504
SinI GGWCC 1 cut(s) 28
SmiMI CAYNNNNRTG 1 cut(s) 56
Sse9I AATT 1 cut(s) 509
SspMI CTAG 2 cut(s) 39, 407
StyD4I CCNGG 2 cut(s) 150, 236
StyI CCWWGG 2 cut(s) 69, 162
TaiI ACGT 1 cut(s) 309
TaqI TCGA 3 cut(s) 300, 324, 504
TasI AATT 1 cut(s) 509
Tru1I TTAA 1 cut(s) 398
Tru9I TTAA 1 cut(s) 398
TseI GCWGC 2 cut(s) 136, 403
TspDTI ATGAA 2 cut(s) 108, 376
TspGWI ACGGA 1 cut(s) 23
Van91I CCANNNNNTGG 1 cut(s) 294
VpaK11BI GGWCC 1 cut(s) 28
XapI RAATTY 1 cut(s) 509
XmiI GTMKAC 1 cut(s) 300
XmnI GAANNNNTTC 1 cut(s) 320
XspI CTAG 2 cut(s) 39, 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.