MD09G1280300.v1.1

Polyamine oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0011767
Physical Location & Seq
Forward (+)
35705060 .. 35711858
6799 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1280300.v1.1.491

Sequence Viewer

Length: 1476 bp
ATGGACCCGAAGGATCCGTTCTCTGCTGATTTCTTTGATGGCTCTGTTTTGTCTCACATTGAGAGGCAACAGAATGTATGCCCTTCTGTTATTGTTATTGGTGGCGGTATATCAGGGATTGCTGCTGCCCGTATTCTCCATGATGCATCTTTTAAGGTGTTTTTGCTCGAATCACGGGACAGACTTGGTGGCCGGATTCATACTGACTACTCATTTGGTTGTCCAGTTGATATGGGAGCATCATGGCTACACGGTGTTTGCAATGAGAATCCTTTGGCTCCACTGATACGCCGCCTAGGACTTACTTTATACCGTACAAGTGGTGACGACTCTGTGTTGTATGACCATGATTTGGAAAGCTTTGCACTTTTTGATATGGATGGCCGTCAAGTTCCTCAAAAGATGGTCGTTGAAGTTGGTGATACTTTCAAGAAGATTCTCAAAGGGACTGAGAAAGTAAGGATTGAGAATTCTGATGACATGTCCGTCTGTCAAGCAATTTCTGTAGTGATGGATAGGCATCCTGAATTAAGACAAAAAGGACTTGCTCACGAAGTGTTACAATGGTACATATGTCGAATGGAAGCATGGTTTGCTGCTGATGCAGATGTAATATCGCTAAAAAACTGGGATCAGCATGTTCTATCTGGTGGTCATGGACTTATGGTGCAAGGCTATGACCCAATAATAAAGGCTCTTGCAAAAGATATTGATGTACGCTTGAATCACAGGGTGACGAAGATATTGTATGGGTCTAATACGGTGATGGCCACAATTGAAGACGGAAGAAACTTCATTGCTGATGCGGCTATAATAACAGTACCCCATGGGATTCTTAAAGCCAATTTGATTGAGTTCGAACCCCAGTTGCCTGAGTGGAAAGTTGCTGCAATTTCTGATCTTGGTGTGGGCAATGAAAATAAAATTGCCTTGCGATTTGAGAAAGTTTTCTGGCCAAATGTAGAGCTCTTGGGTATTGTTGCACCAAATTCTTATGGATGTGGTTATTTTCTCAATCTTCACAAGGTCACAGGCCATCCAGTCCTTGTATATATGGCCGCTGGAAGGTTTGCATATGACCTTGAAAAACTAACAGATGACGCTGCTGTTAATTTTGTGATGTTGCAGCTTAAGAAGATGATGTCTGATGCCACTGAACCAGTTCAATATCTGGTATCACGATGGGGAACAGACGTGAATTCTCTTGGATGTTACGCGTTGGATTTGGTTGGAAAGCCAGGAGATATATACGAGCGGCTTCGGGCACCTTTGGGTAATCTATTTTTTGGTGGGGAAGCTGTTAGCATGGACCACCAAGGATCTGTACACGGAGCTTACTCCGCAGGAGTTATGGCTGCTGAGAACTGTCAGCGGCATCTGTTACAGGAACTCGGAAATTTGGAGACCCGTCAGCATGCTTACCTTAGGGATGAAGTGCTTGAAGGAACAATTCCCATCCAAATCTCAAGGATGTGA

Protein Analysis

492

Amino Acids

54.47

Weight (kDa)

5.51

Isoelectric Point (pI)

32.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_8 PF13450 32 - 98 1.2e-13 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 37 - 455 1.4e-81 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011767)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65840
fragaria_vesca FvH4_1g27430
malus_domestica MD09G1280300.v1.1 MD17G1287500.v1.1
prunus_persica Prupe.3G013800_v2.0.a1 Prupe.3G013800_v2.0.a1
pyrus_communis pycom09g18840 pycom17g27050
rosa_chinensis RchiOBHm_Chr3g0488611
rosa_laevigata RLG00000022940
rosa_multiflora Rmu_sc0005363.1_g000049
rosa_roxburghii Rroxscaffold_6G00394100
rosa_rugosa Rorug03G0239500 Rorug03G0239600
rosa_samantha Rh3AG288500 Rh3BG325200 Rh3CG322800 Rh3DG321500
rosa_wichuraiana Rw3G025530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 485
AccB1I GGYRCC 1 cut(s) 1264
AccB7I CCANNNNNTGG 1 cut(s) 352
AccBSI CCGCTC 1 cut(s) 1255
AccII CGCG 1 cut(s) 1217
AciI CCGC 7 cut(s) 105, 292, 806, 1059, 1255, 1341, 1372
AclWI GGATC 4 cut(s) 8, 21, 639, 1327
AcoI YGGCCR 5 cut(s) 190, 382, 768, 953, 1056
AcsI RAATTY 4 cut(s) 469, 988, 1198, 1396
AdeI CACNNNGTG 2 cut(s) 556, 733
AfaI GTAC 5 cut(s) 316, 569, 717, 822, 1326
AfiI CCNNNNNNNGG 2 cut(s) 352, 1065
AflII CTTAAG 1 cut(s) 1130
AflIII ACRYGT 2 cut(s) 480, 1215
AgsI TTSAA 7 cut(s) 413, 430, 724, 779, 1085, 1166, 1442
AjiI CACGTC 1 cut(s) 1195
AjnI CCWGG 1 cut(s) 1237
AluBI AGCT 5 cut(s) 360, 967, 1129, 1298, 1334
AluI AGCT 5 cut(s) 360, 967, 1129, 1298, 1334
Alw21I GWGCWC 1 cut(s) 969
Alw26I GTCTC 2 cut(s) 57, 1397
AlwI GGATC 4 cut(s) 8, 21, 639, 1327
AoxI GGCC 6 cut(s) 190, 382, 768, 953, 1033, 1056
ApeKI GCWGC 7 cut(s) 122, 125, 596, 887, 1103, 1126, 1355
ApoI RAATTY 4 cut(s) 469, 988, 1198, 1396
ArsI GACNNNNNNTTYG 2 cut(s) 197, 229
Asp700I GAANNNNTTC 2 cut(s) 947, 1161
AspA2I CCTAGG 1 cut(s) 295
AspS9I GGNCC 2 cut(s) 4, 1309
AsuHPI GGTGA 4 cut(s) 335, 431, 745, 775
AsuII TTCGAA 1 cut(s) 858
AvaII GGWCC 2 cut(s) 4, 1309
AvrII CCTAGG 1 cut(s) 295
AxyI CCTNAGG 1 cut(s) 1424
BaeGI GKGCMC 1 cut(s) 1267
BaeI ACNNNNGTAYC 2 cut(s) 559, 592
BalI TGGCCA 2 cut(s) 770, 955
BamHI GGATCC 1 cut(s) 13
BanI GGYRCC 1 cut(s) 1264
BanII GRGCYC 1 cut(s) 969
BbsI GAAGAC 1 cut(s) 786
Bbv12I GWGCWC 1 cut(s) 969
BbvI GCAGC 7 cut(s) 109, 112, 583, 874, 1090, 1138, 1342
BccI CCATC 8 cut(s) 32, 374, 397, 505, 760, 1044, 1176, 1463
BceAI ACGGC 1 cut(s) 369
BciT130I CCWGG 1 cut(s) 1239
BcoDI GTCTC 2 cut(s) 57, 1397
BfaI CTAG 1 cut(s) 296
BfmI CTRYAG 1 cut(s) 504
BfrI CTTAAG 1 cut(s) 1130
BlnI CCTAGG 1 cut(s) 295
Bme1390I CCNGG 1 cut(s) 1239
Bme18I GGWCC 2 cut(s) 4, 1309
BmgBI CACGTC 1 cut(s) 1195
BmgT120I GGNCC 2 cut(s) 4, 1309
BmiI GGNNCC 4 cut(s) 6, 15, 279, 1266
BmrFI CCNGG 1 cut(s) 1239
BmrI ACTGGG 2 cut(s) 637, 859
BmsI GCATC 8 cut(s) 133, 155, 248, 529, 592, 793, 1138, 1384
BmuI ACTGGG 2 cut(s) 637, 859
BpiI GAAGAC 1 cut(s) 786
Bpu14I TTCGAA 1 cut(s) 858
BpuEI CTTGAG 1 cut(s) 1450
BsaBI GATNNNNATC 1 cut(s) 519
BsaI GGTCTC 1 cut(s) 1397
BsaJI CCNNGG 3 cut(s) 295, 826, 1315
Bsc4I CCNNNNNNNGG 2 cut(s) 352, 1065
Bse1I ACTGG 5 cut(s) 224, 632, 865, 1040, 1160
Bse21I CCTNAGG 1 cut(s) 1424
Bse3DI GCAATG 3 cut(s) 268, 795, 919
Bse8I GATNNNNATC 1 cut(s) 519
BseBI CCWGG 1 cut(s) 1239
BseDI CCNNGG 3 cut(s) 295, 826, 1315
BseGI GGATG 8 cut(s) 385, 520, 1004, 1036, 1214, 1435, 1455, 1476
BseJI GATNNNNATC 1 cut(s) 519
BseLI CCNNNNNNNGG 2 cut(s) 352, 1065
BseMI GCAATG 3 cut(s) 268, 795, 919
BseMII CTCAG 3 cut(s) 441, 864, 1350
BseNI ACTGG 5 cut(s) 224, 632, 865, 1040, 1160
BseSI GKGCMC 1 cut(s) 1267
BseXI GCAGC 7 cut(s) 109, 112, 583, 874, 1090, 1138, 1342
Bsh1236I CGCG 1 cut(s) 1217
BshFI GGCC 6 cut(s) 192, 384, 770, 955, 1035, 1058
BshNI GGYRCC 1 cut(s) 1264
BsiHKAI GWGCWC 1 cut(s) 969
BsiSI CCGG 1 cut(s) 193
BslFI GGGAC 2 cut(s) 191, 460
BslI CCNNNNNNNGG 2 cut(s) 352, 1065
BsmAI GTCTC 2 cut(s) 57, 1397
BsmFI GGGAC 2 cut(s) 191, 460
BsnI GGCC 6 cut(s) 192, 384, 770, 955, 1035, 1058
Bso31I GGTCTC 1 cut(s) 1397
Bsp119I TTCGAA 1 cut(s) 858
Bsp1286I GDGCHC 2 cut(s) 969, 1267
Bsp1407I TGTACA 1 cut(s) 1324
Bsp143I GATC 4 cut(s) 13, 631, 898, 1319
Bsp19I CCATGG 1 cut(s) 826
BspACI CCGC 7 cut(s) 105, 292, 806, 1059, 1255, 1341, 1372
BspANI GGCC 6 cut(s) 192, 384, 770, 955, 1035, 1058
BspCNI CTCAG 3 cut(s) 442, 865, 1351
BspFNI CGCG 1 cut(s) 1217
BspLI GGNNCC 4 cut(s) 6, 15, 279, 1266
BspPI GGATC 4 cut(s) 8, 21, 639, 1327
BspT104I TTCGAA 1 cut(s) 858
BspT107I GGYRCC 1 cut(s) 1264
BspTI CTTAAG 1 cut(s) 1130
BspTNI GGTCTC 1 cut(s) 1397
BsrBI CCGCTC 1 cut(s) 1255
BsrDI GCAATG 3 cut(s) 268, 795, 919
BsrGI TGTACA 1 cut(s) 1324
BsrI ACTGG 5 cut(s) 224, 632, 865, 1040, 1160
BssECI CCNNGG 3 cut(s) 295, 826, 1315
BssMI GATC 4 cut(s) 13, 631, 898, 1319
BssT1I CCWWGG 3 cut(s) 295, 826, 1315
Bst2UI CCWGG 1 cut(s) 1239
Bst4CI ACNGT 5 cut(s) 254, 314, 763, 820, 1367
BstAFI CTTAAG 1 cut(s) 1130
BstAPI GCANNNNNTGC 1 cut(s) 593
BstAUI TGTACA 1 cut(s) 1324
BstBI TTCGAA 1 cut(s) 858
BstC8I GCNNGC 1 cut(s) 1416
BstDEI CTNAG 4 cut(s) 450, 873, 1359, 1424
BstDSI CCRYGG 1 cut(s) 826
BstF5I GGATG 8 cut(s) 385, 520, 1004, 1036, 1214, 1435, 1455, 1476
BstFNI CGCG 1 cut(s) 1217
BstKTI GATC 4 cut(s) 16, 634, 901, 1322
BstMAI GTCTC 2 cut(s) 57, 1397
BstMBI GATC 4 cut(s) 13, 631, 898, 1319
BstMWI GCNNNNNNNGC 4 cut(s) 593, 602, 806, 1340
BstNI CCWGG 1 cut(s) 1239
BstNSI RCATGY 3 cut(s) 484, 641, 1418
BstSCI CCNGG 1 cut(s) 1237
BstSFI CTRYAG 1 cut(s) 504
BstSLI GKGCMC 1 cut(s) 1267
BstUI CGCG 1 cut(s) 1217
BstV1I GCAGC 7 cut(s) 109, 112, 583, 874, 1090, 1138, 1342
BstV2I GAAGAC 1 cut(s) 786
BstX2I RGATCY 2 cut(s) 13, 1319
BstYI RGATCY 2 cut(s) 13, 1319
Bsu36I CCTNAGG 1 cut(s) 1424
BsuRI GGCC 6 cut(s) 192, 384, 770, 955, 1035, 1058
BtgI CCRYGG 1 cut(s) 826
BtrI CACGTC 1 cut(s) 1195
BtsCI GGATG 8 cut(s) 385, 520, 1004, 1036, 1214, 1435, 1455, 1476
BtsIMutI CAGTG 2 cut(s) 281, 1152
Cac8I GCNNGC 1 cut(s) 1416
Cfr13I GGNCC 2 cut(s) 4, 1309
CseI GACGC 1 cut(s) 1109
Csp6I GTAC 5 cut(s) 315, 568, 716, 821, 1325
CviQI GTAC 5 cut(s) 315, 568, 716, 821, 1325
DdeI CTNAG 4 cut(s) 450, 873, 1359, 1424
DpnI GATC 4 cut(s) 15, 633, 900, 1321
DpnII GATC 4 cut(s) 13, 631, 898, 1319
DraIII CACNNNGTG 2 cut(s) 556, 733
DrdI GACNNNNNNGTC 1 cut(s) 485
DseDI GACNNNNNNGTC 1 cut(s) 485
EaeI YGGCCR 5 cut(s) 190, 382, 768, 953, 1056
Ecl136II GAGCTC 1 cut(s) 967
Eco130I CCWWGG 3 cut(s) 295, 826, 1315
Eco24I GRGCYC 1 cut(s) 969
Eco31I GGTCTC 1 cut(s) 1397
Eco47I GGWCC 2 cut(s) 4, 1309
Eco53kI GAGCTC 1 cut(s) 967
Eco81I CCTNAGG 1 cut(s) 1424
EcoICRI GAGCTC 1 cut(s) 967
EcoRI GAATTC 2 cut(s) 469, 1198
EcoRII CCWGG 1 cut(s) 1237
EcoT14I CCWWGG 3 cut(s) 295, 826, 1315
EcoT22I ATGCAT 1 cut(s) 148
EcoT38I GRGCYC 1 cut(s) 969
ErhI CCWWGG 3 cut(s) 295, 826, 1315
FaqI GGGAC 2 cut(s) 191, 460
FauNDI CATATG 2 cut(s) 572, 1075
FokI GGATG 7 cut(s) 392, 507, 1011, 1023, 1221, 1442, 1442
FriOI GRGCYC 1 cut(s) 969
FspBI CTAG 1 cut(s) 296
HaeIII GGCC 6 cut(s) 192, 384, 770, 955, 1035, 1058
HapII CCGG 1 cut(s) 193
HgaI GACGC 1 cut(s) 1109
HindIII AAGCTT 1 cut(s) 358
HinfI GANTC 7 cut(s) 170, 196, 268, 329, 436, 724, 832
HpaII CCGG 1 cut(s) 193
HphI GGTGA 4 cut(s) 335, 431, 745, 775
Hpy166II GTNNAC 1 cut(s) 1327
Hpy188I TCNGA 4 cut(s) 475, 898, 1147, 1394
Hpy188III TCNNGA 4 cut(s) 430, 524, 551, 1179
Hpy8I GTNNAC 1 cut(s) 1327
HpyAV CCTTC 4 cut(s) 4, 93, 1059, 1436
HpyCH4III ACNGT 5 cut(s) 254, 314, 763, 820, 1367
HpyCH4IV ACGT 1 cut(s) 1194
HpyF10VI GCNNNNNNNGC 4 cut(s) 593, 602, 806, 1340
HpyF3I CTNAG 4 cut(s) 450, 873, 1359, 1424
HpySE526I ACGT 1 cut(s) 1194
Kzo9I GATC 4 cut(s) 13, 631, 898, 1319
LmnI GCTCC 3 cut(s) 236, 283, 1331
Lsp1109I GCAGC 7 cut(s) 109, 112, 583, 874, 1090, 1138, 1342
LweI GCATC 8 cut(s) 133, 155, 248, 529, 592, 793, 1138, 1384
MaeI CTAG 1 cut(s) 296
MaeII ACGT 1 cut(s) 1194
MaeIII GTNAC 6 cut(s) 323, 558, 733, 1027, 1211, 1380
MalI GATC 4 cut(s) 15, 633, 900, 1321
MbiI CCGCTC 1 cut(s) 1255
MboI GATC 4 cut(s) 13, 631, 898, 1319
MboII GAAGA 6 cut(s) 445, 751, 791, 798, 1010, 1147
MfeI CAATTG 1 cut(s) 774
MflI RGATCY 2 cut(s) 13, 1319
MhlI GDGCHC 2 cut(s) 969, 1267
MlsI TGGCCA 2 cut(s) 770, 955
MluI ACGCGT 1 cut(s) 1215
MluNI TGGCCA 2 cut(s) 770, 955
MlyI GAGTC 1 cut(s) 323
MmeI TCCRAC 2 cut(s) 1200, 1210
MnlI CCTC 2 cut(s) 57, 405
Mox20I TGGCCA 2 cut(s) 770, 955
Mph1103I ATGCAT 1 cut(s) 148
MroXI GAANNNNTTC 2 cut(s) 947, 1161
MscI TGGCCA 2 cut(s) 770, 955
MseI TTAA 5 cut(s) 153, 530, 837, 1110, 1131
Msp20I TGGCCA 2 cut(s) 770, 955
MspA1I CMGCKG 2 cut(s) 1061, 1372
MspCI CTTAAG 1 cut(s) 1130
MspI CCGG 1 cut(s) 193
MspR9I CCNGG 1 cut(s) 1239
MunI CAATTG 1 cut(s) 774
MvaI CCWGG 1 cut(s) 1239
MvnI CGCG 1 cut(s) 1217
MwoI GCNNNNNNNGC 4 cut(s) 593, 602, 806, 1340
NcoI CCATGG 1 cut(s) 826
NdeI CATATG 2 cut(s) 572, 1075
NdeII GATC 4 cut(s) 13, 631, 898, 1319
NlaIV GGNNCC 4 cut(s) 6, 15, 279, 1266
NmuCI GTSAC 3 cut(s) 323, 733, 1027
NsiI ATGCAT 1 cut(s) 148
NspI RCATGY 3 cut(s) 484, 641, 1418
NspV TTCGAA 1 cut(s) 858
PaeI GCATGC 1 cut(s) 1418
PciI ACATGT 1 cut(s) 480
PdmI GAANNNNTTC 2 cut(s) 947, 1161
PfeI GAWTC 6 cut(s) 170, 196, 268, 436, 724, 832
PflMI CCANNNNNTGG 1 cut(s) 352
PleI GAGTC 1 cut(s) 323
PpsI GAGTC 1 cut(s) 323
PscI ACATGT 1 cut(s) 480
Psp124BI GAGCTC 1 cut(s) 969
Psp6I CCWGG 1 cut(s) 1237
PspGI CCWGG 1 cut(s) 1237
PspN4I GGNNCC 4 cut(s) 6, 15, 279, 1266
PspPI GGNCC 2 cut(s) 4, 1309
PsuI RGATCY 2 cut(s) 13, 1319
RsaI GTAC 5 cut(s) 316, 569, 717, 822, 1326
RsaNI GTAC 5 cut(s) 315, 568, 716, 821, 1325
SacI GAGCTC 1 cut(s) 969
SaqAI TTAA 5 cut(s) 153, 530, 837, 1110, 1131
Sau3AI GATC 4 cut(s) 13, 631, 898, 1319
Sau96I GGNCC 2 cut(s) 4, 1309
SchI GAGTC 1 cut(s) 323
ScrFI CCNGG 1 cut(s) 1239
SduI GDGCHC 2 cut(s) 969, 1267
SfaNI GCATC 8 cut(s) 133, 155, 248, 529, 592, 793, 1138, 1384
SfcI CTRYAG 1 cut(s) 504
SfuI TTCGAA 1 cut(s) 858
SinI GGWCC 2 cut(s) 4, 1309
SmlI CTYRAG 2 cut(s) 1130, 1465
SmoI CTYRAG 2 cut(s) 1130, 1465
SphI GCATGC 1 cut(s) 1418
SsiI CCGC 7 cut(s) 105, 292, 806, 1059, 1255, 1341, 1372
SspMI CTAG 1 cut(s) 296
SstI GAGCTC 1 cut(s) 969
StyD4I CCNGG 1 cut(s) 1237
StyI CCWWGG 3 cut(s) 295, 826, 1315
TaaI ACNGT 5 cut(s) 254, 314, 763, 820, 1367
TaiI ACGT 1 cut(s) 1197
TaqI TCGA 3 cut(s) 168, 577, 858
TatI WGTACW 1 cut(s) 1324
TauI GCSGC 5 cut(s) 294, 809, 1061, 1258, 1375
TfiI GAWTC 6 cut(s) 170, 196, 268, 436, 724, 832
Tru1I TTAA 5 cut(s) 153, 530, 837, 1110, 1131
Tru9I TTAA 5 cut(s) 153, 530, 837, 1110, 1131
TscAI CASTG 2 cut(s) 288, 1159
TseFI GTSAC 3 cut(s) 323, 733, 1027
TseI GCWGC 7 cut(s) 122, 125, 596, 887, 1103, 1126, 1355
Tsp45I GTSAC 3 cut(s) 323, 733, 1027
TspDTI ATGAA 4 cut(s) 188, 784, 930, 1446
TspGWI ACGGA 4 cut(s) 6, 475, 798, 1344
TspRI CASTG 2 cut(s) 288, 1159
Van91I CCANNNNNTGG 1 cut(s) 352
Vha464I CTTAAG 1 cut(s) 1130
VpaK11BI GGWCC 2 cut(s) 4, 1309
XapI RAATTY 4 cut(s) 469, 988, 1198, 1396
XceI RCATGY 3 cut(s) 484, 641, 1418
XmaJI CCTAGG 1 cut(s) 295
XmnI GAANNNNTTC 2 cut(s) 947, 1161
XspI CTAG 1 cut(s) 296
Zsp2I ATGCAT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.