Rw3G025530

Polyamine oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0011767
Physical Location & Seq
Reverse (-)
34245818 .. 34250421
4604 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G025530.1

Sequence Viewer

Length: 1485 bp
ATGGACCCCAAAGATCCGTTCTTTACTGATTTGATTGACGACTCAATTCCTTGTCGCATTGAGAGGCAGCACAATGCATCATCATGGCCTTCTGTTATTGTTGTTGGTGGTGGTATATCTGGTGTTGCAGCGGCTCGCCTTCTCCATGACGCTTCCTTTAAGGTGACATTGCTCGAATCACGAGATAGACTCGGTGGCCGGATTCTTACTGATTACTCATTTGGTTGTCCGGTAGATATGGGAGCATCATGGCTACATGGTGTTTGCAATGAGAATCCCTTGGCTCCACTTATACGCCGTCTAGGACTTACTTTATATCGTACTAGCGGCGACAACTCTGTGTTGTATGATCATGATTTGGAAAGCTTTGCGCTTTTCGATATGGACGGCAAACAAGTTCCTCAGCAGATGGTCGTTGAAGTTGGTGATATATTCAAGAGAATTCTCACTGAGACAGAGAAAGTAAGGAATGAACATACTGATGACATGTCTGTTCACCAAGCAATAATGATTGTCTTGAATAGGCATCCAGAGTTAAGACAAGAAGGACTTGCTCAAGCAGTGTTGCAATGGTACATGTGTCGAATGGAAGCATGGTTTGCTGCAGATGCAGATATGATATCACTGAAAAACTGGGATCAGGAGCATGTTCTCTCTGGTGGTCATGGACTTATGGTGGAAGGTTATGATCCCATAATAAAGACTCTTGCAAAAGATATCGATGTGCGGTTGAGTCACAGGGTCACAAAGATATCAAATGGTTGTAATAAGGTGATGCTCACAACTGAAGATGGCAGAAACTTCATTGCAGATGCGGCTATCATAACCGTACCTCTTGGGGTTCTTAAAGCCAAGTTGATCGAGTTCGAACCCACCTTACCAGAGTGGAAAATGTCTGCAATTTCTGATCTTGGGGTGGGCAATGAAAACAAAATTGCCTTACGATTTGATAAAGTTTTCTGGCCGAATGTAGAGCTATTAGGAGTTGTTGCACCTTCTTCCTATGGATGCGCTTATTTTCTCAATCTTCACAAGACAACGGGGCAACCAGTCCTCGTCTATATGTGTGCTGGAAGGTTTGCATATGACCTTGAAAATCTAGATGATGTTACTGCTGTTAAATTTGTGAATGATCAGCTCAAGAATATGTTACCCGAAGCAACTGAGCCGGTTCAATATCTTGTATCACATTGGGGAACAGACCTGAATTCCCTTGGATGTTACTCTTTGGATTTGGTTGGAAAGCCAGCAGATGTATACGAGAGGCTTCGAGCACCTTTGGGTAACCTTTTCTTTGGAGGGGAAGCTGTCAGCATGGATCATCAAGGATCTGTACATGGGGCTTATGCAGCAGGATTAATGGCAGCTGAAAACTGTCAGAATCATCTCTTGGAGAAACTTGGTAGATTGGAAAAGTTGCAGCGTGTTTATGTTGGCGATGAAGTGCTCGAAGCTATTGTTCCGCTGCAAATCTCAAGGATTTGA

Protein Analysis

494

Amino Acids

54.92

Weight (kDa)

5.18

Isoelectric Point (pI)

32.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_8 PF13450 34 - 100 1.2e-14 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 39 - 458 1e-85 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011767)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65840
fragaria_vesca FvH4_1g27430
malus_domestica MD09G1280300.v1.1 MD17G1287500.v1.1
prunus_persica Prupe.3G013800_v2.0.a1 Prupe.3G013800_v2.0.a1
pyrus_communis pycom09g18840 pycom17g27050
rosa_chinensis RchiOBHm_Chr3g0488611
rosa_laevigata RLG00000022940
rosa_multiflora Rmu_sc0005363.1_g000049
rosa_roxburghii Rroxscaffold_6G00394100
rosa_rugosa Rorug03G0239500 Rorug03G0239600
rosa_samantha Rh3AG288500 Rh3BG325200 Rh3CG322800 Rh3DG321500
rosa_wichuraiana Rw3G025530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1257
AciI CCGC 5 cut(s) 131, 327, 727, 815, 1463
AclWI GGATC 5 cut(s) 8, 645, 683, 1326, 1336
AcoI YGGCCR 2 cut(s) 196, 962
AcsI RAATTY 3 cut(s) 441, 1121, 1207
AcuI CTGAAG 1 cut(s) 807
AfaI GTAC 4 cut(s) 322, 575, 831, 1335
AflIII ACRYGT 2 cut(s) 486, 576
AgsI TTSAA 5 cut(s) 419, 436, 520, 1094, 1175
AjuI GAANNNNNNNTTGG 3 cut(s) 34, 1373, 1405
AluBI AGCT 6 cut(s) 366, 976, 1138, 1307, 1367, 1454
AluI AGCT 6 cut(s) 366, 976, 1138, 1307, 1367, 1454
Alw21I GWGCWC 2 cut(s) 1276, 1449
Alw26I GTCTC 1 cut(s) 446
AlwI GGATC 5 cut(s) 8, 645, 683, 1326, 1336
AoxI GGCC 3 cut(s) 86, 196, 962
ApeKI GCWGC 7 cut(s) 67, 128, 602, 1349, 1364, 1420, 1465
ApoI RAATTY 3 cut(s) 441, 1121, 1207
AseI ATTAAT 1 cut(s) 1358
AspLEI GCGC 2 cut(s) 373, 1013
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 4 cut(s) 175, 437, 488, 784
AsuII TTCGAA 1 cut(s) 867
AvaII GGWCC 1 cut(s) 4
BaeI ACNNNNGTAYC 2 cut(s) 565, 598
BauI CACGAG 1 cut(s) 180
Bbv12I GWGCWC 2 cut(s) 1276, 1449
BbvCI CCTCAGC 1 cut(s) 402
BbvI GCAGC 7 cut(s) 79, 140, 589, 1361, 1376, 1432, 1452
BccI CCATC 2 cut(s) 403, 785
BceAI ACGGC 2 cut(s) 282, 403
BclI TGATCA 2 cut(s) 349, 1132
BcoDI GTCTC 1 cut(s) 446
BfaI CTAG 3 cut(s) 302, 324, 1100
BfmI CTRYAG 1 cut(s) 603
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 2 cut(s) 6, 285
BmrI ACTGGG 1 cut(s) 643
BmsI GCATC 7 cut(s) 86, 254, 535, 598, 765, 802, 998
BmuI ACTGGG 1 cut(s) 643
BplI GAGNNNNNCTC 2 cut(s) 174, 206
Bpu10I CCTNAGC 1 cut(s) 402
Bpu14I TTCGAA 1 cut(s) 867
BpuEI CTTGAG 3 cut(s) 540, 1124, 1459
Bsa29I ATCGAT 1 cut(s) 720
BsaJI CCNNGG 2 cut(s) 279, 1213
BsaWI WCCGGW 1 cut(s) 229
Bse118I RCCGGY 1 cut(s) 1168
Bse1I ACTGG 2 cut(s) 638, 1049
Bse3DI GCAATG 5 cut(s) 167, 274, 575, 804, 928
BseCI ATCGAT 1 cut(s) 720
BseDI CCNNGG 2 cut(s) 279, 1213
BseGI GGATG 3 cut(s) 526, 1013, 1223
BseMI GCAATG 5 cut(s) 167, 274, 575, 804, 928
BseMII CTCAG 3 cut(s) 416, 441, 1155
BseNI ACTGG 2 cut(s) 638, 1049
BseXI GCAGC 7 cut(s) 79, 140, 589, 1361, 1376, 1432, 1452
BshFI GGCC 3 cut(s) 88, 198, 964
BshVI ATCGAT 1 cut(s) 720
BsiHKAI GWGCWC 2 cut(s) 1276, 1449
BsiSI CCGG 3 cut(s) 199, 230, 1169
BsmAI GTCTC 1 cut(s) 446
BsnI GGCC 3 cut(s) 88, 198, 964
Bsp119I TTCGAA 1 cut(s) 867
Bsp1286I GDGCHC 2 cut(s) 1276, 1449
Bsp1407I TGTACA 1 cut(s) 1333
Bsp143I GATC 9 cut(s) 13, 349, 637, 688, 858, 907, 1132, 1318, 1328
BspACI CCGC 5 cut(s) 131, 327, 727, 815, 1463
BspANI GGCC 3 cut(s) 88, 198, 964
BspCNI CTCAG 3 cut(s) 415, 442, 1156
BspDI ATCGAT 1 cut(s) 720
BspHI TCATGA 1 cut(s) 352
BspLI GGNNCC 2 cut(s) 6, 285
BspMAI CTGCAG 1 cut(s) 607
BspPI GGATC 5 cut(s) 8, 645, 683, 1326, 1336
BspT104I TTCGAA 1 cut(s) 867
BsrDI GCAATG 5 cut(s) 167, 274, 575, 804, 928
BsrFI RCCGGY 1 cut(s) 1168
BsrGI TGTACA 1 cut(s) 1333
BsrI ACTGG 2 cut(s) 638, 1049
BssAI RCCGGY 1 cut(s) 1168
BssECI CCNNGG 2 cut(s) 279, 1213
BssMI GATC 9 cut(s) 13, 349, 637, 688, 858, 907, 1132, 1318, 1328
BssNAI GTATAC 1 cut(s) 1258
BssSI CACGAG 1 cut(s) 180
BssT1I CCWWGG 2 cut(s) 279, 1213
Bst1107I GTATAC 1 cut(s) 1258
Bst2BI CACGAG 1 cut(s) 180
Bst4CI ACNGT 2 cut(s) 829, 1376
BstAPI GCANNNNNTGC 1 cut(s) 599
BstAUI TGTACA 1 cut(s) 1333
BstBI TTCGAA 1 cut(s) 867
BstC8I GCNNGC 2 cut(s) 136, 1248
BstDEI CTNAG 3 cut(s) 402, 450, 1164
BstEII GGTNACC 1 cut(s) 1283
BstF5I GGATG 3 cut(s) 526, 1013, 1223
BstHHI GCGC 2 cut(s) 373, 1013
BstKTI GATC 9 cut(s) 16, 352, 640, 691, 861, 910, 1135, 1321, 1331
BstMAI GTCTC 1 cut(s) 446
BstMBI GATC 9 cut(s) 13, 349, 637, 688, 858, 907, 1132, 1318, 1328
BstMWI GCNNNNNNNGC 4 cut(s) 599, 608, 815, 1349
BstNSI RCATGY 3 cut(s) 490, 580, 650
BstPI GGTNACC 1 cut(s) 1283
BstSFI CTRYAG 1 cut(s) 603
BstV1I GCAGC 7 cut(s) 79, 140, 589, 1361, 1376, 1432, 1452
BstX2I RGATCY 2 cut(s) 13, 1328
BstYI RGATCY 2 cut(s) 13, 1328
BstZ17I GTATAC 1 cut(s) 1258
Bsu15I ATCGAT 1 cut(s) 720
BsuRI GGCC 3 cut(s) 88, 198, 964
BsuTUI ATCGAT 1 cut(s) 720
BtgZI GCGATG 1 cut(s) 1452
BtsCI GGATG 3 cut(s) 526, 1013, 1223
BtsI GCAGTG 1 cut(s) 567
BtsIMutI CAGTG 3 cut(s) 447, 567, 623
Cac8I GCNNGC 2 cut(s) 136, 1248
CciI TCATGA 1 cut(s) 352
CfoI GCGC 2 cut(s) 373, 1013
Cfr10I RCCGGY 1 cut(s) 1168
Cfr13I GGNCC 1 cut(s) 4
ClaI ATCGAT 1 cut(s) 720
CseI GACGC 1 cut(s) 158
Csp6I GTAC 4 cut(s) 321, 574, 830, 1334
CviQI GTAC 4 cut(s) 321, 574, 830, 1334
DdeI CTNAG 3 cut(s) 402, 450, 1164
DpnI GATC 9 cut(s) 15, 351, 639, 690, 860, 909, 1134, 1320, 1330
DpnII GATC 9 cut(s) 13, 349, 637, 688, 858, 907, 1132, 1318, 1328
EaeI YGGCCR 2 cut(s) 196, 962
Eco130I CCWWGG 2 cut(s) 279, 1213
Eco32I GATATC 3 cut(s) 621, 718, 753
Eco47I GGWCC 1 cut(s) 4
Eco57I CTGAAG 1 cut(s) 807
Eco91I GGTNACC 1 cut(s) 1283
EcoO65I GGTNACC 1 cut(s) 1283
EcoRI GAATTC 2 cut(s) 441, 1207
EcoRV GATATC 3 cut(s) 621, 718, 753
EcoT14I CCWWGG 2 cut(s) 279, 1213
EcoT22I ATGCAT 1 cut(s) 79
ErhI CCWWGG 2 cut(s) 279, 1213
FalI AAGNNNNNCTT 2 cut(s) 534, 566
FauNDI CATATG 1 cut(s) 1084
FbaI TGATCA 2 cut(s) 349, 1132
FblI GTMKAC 1 cut(s) 1257
FokI GGATG 3 cut(s) 513, 1020, 1230
FspBI CTAG 3 cut(s) 302, 324, 1100
GlaI GCGC 2 cut(s) 372, 1012
HaeIII GGCC 3 cut(s) 88, 198, 964
HapII CCGG 3 cut(s) 199, 230, 1169
HgaI GACGC 1 cut(s) 158
HhaI GCGC 2 cut(s) 373, 1013
Hin6I GCGC 2 cut(s) 371, 1011
HinP1I GCGC 2 cut(s) 371, 1011
HindIII AAGCTT 1 cut(s) 364
HinfI GANTC 8 cut(s) 41, 176, 189, 202, 274, 703, 733, 1381
HpaII CCGG 3 cut(s) 199, 230, 1169
HphI GGTGA 4 cut(s) 175, 437, 488, 784
Hpy166II GTNNAC 2 cut(s) 496, 1258
Hpy188I TCNGA 2 cut(s) 907, 1380
Hpy188III TCNNGA 8 cut(s) 180, 353, 436, 517, 530, 641, 1100, 1141
Hpy8I GTNNAC 2 cut(s) 496, 1258
HpyAV CCTTC 6 cut(s) 99, 149, 539, 674, 1005, 1068
HpyCH4III ACNGT 2 cut(s) 829, 1376
HpyF10VI GCNNNNNNNGC 4 cut(s) 599, 608, 815, 1349
HpyF3I CTNAG 3 cut(s) 402, 450, 1164
HspAI GCGC 2 cut(s) 371, 1011
Ksp22I TGATCA 2 cut(s) 349, 1132
Kzo9I GATC 9 cut(s) 13, 349, 637, 688, 858, 907, 1132, 1318, 1328
LmnI GCTCC 3 cut(s) 242, 289, 643
Lsp1109I GCAGC 7 cut(s) 79, 140, 589, 1361, 1376, 1432, 1452
LweI GCATC 7 cut(s) 86, 254, 535, 598, 765, 802, 998
MaeI CTAG 3 cut(s) 302, 324, 1100
MaeIII GTNAC 7 cut(s) 163, 734, 742, 1108, 1149, 1220, 1283
MalI GATC 9 cut(s) 15, 351, 639, 690, 860, 909, 1134, 1320, 1330
MboI GATC 9 cut(s) 13, 349, 637, 688, 858, 907, 1132, 1318, 1328
MboII GAAGA 3 cut(s) 800, 990, 1019
MflI RGATCY 2 cut(s) 13, 1328
MhlI GDGCHC 2 cut(s) 1276, 1449
MluCI AATT 6 cut(s) 45, 441, 900, 933, 1121, 1207
MlyI GAGTC 4 cut(s) 35, 183, 697, 742
MmeI TCCRAC 1 cut(s) 1219
MnlI CCTC 6 cut(s) 57, 411, 843, 1064, 1257, 1292
Mph1103I ATGCAT 1 cut(s) 79
MseI TTAA 5 cut(s) 159, 536, 846, 1119, 1358
MslI CAYNNNNRTG 2 cut(s) 82, 480
MspA1I CMGCKG 3 cut(s) 131, 1367, 1465
MspI CCGG 3 cut(s) 199, 230, 1169
MwoI GCNNNNNNNGC 4 cut(s) 599, 608, 815, 1349
NdeI CATATG 1 cut(s) 1084
NdeII GATC 9 cut(s) 13, 349, 637, 688, 858, 907, 1132, 1318, 1328
NlaIV GGNNCC 2 cut(s) 6, 285
NmuCI GTSAC 3 cut(s) 163, 734, 742
NsiI ATGCAT 1 cut(s) 79
NspI RCATGY 3 cut(s) 490, 580, 650
NspV TTCGAA 1 cut(s) 867
PagI TCATGA 1 cut(s) 352
PciI ACATGT 2 cut(s) 486, 576
PfeI GAWTC 4 cut(s) 176, 202, 274, 1381
PleI GAGTC 4 cut(s) 35, 183, 697, 741
PpsI GAGTC 4 cut(s) 35, 183, 697, 741
PscI ACATGT 2 cut(s) 486, 576
PshBI ATTAAT 1 cut(s) 1358
PspEI GGTNACC 1 cut(s) 1283
PspN4I GGNNCC 2 cut(s) 6, 285
PspPI GGNCC 1 cut(s) 4
PsrI GAACNNNNNNTAC 2 cut(s) 861, 893
PstI CTGCAG 1 cut(s) 607
PsuI RGATCY 2 cut(s) 13, 1328
PvuII CAGCTG 1 cut(s) 1367
RsaI GTAC 4 cut(s) 322, 575, 831, 1335
RsaNI GTAC 4 cut(s) 321, 574, 830, 1334
RseI CAYNNNNRTG 2 cut(s) 82, 480
SaqAI TTAA 5 cut(s) 159, 536, 846, 1119, 1358
Sau3AI GATC 9 cut(s) 13, 349, 637, 688, 858, 907, 1132, 1318, 1328
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 4 cut(s) 35, 183, 697, 742
SduI GDGCHC 2 cut(s) 1276, 1449
SfaNI GCATC 7 cut(s) 86, 254, 535, 598, 765, 802, 998
SfcI CTRYAG 1 cut(s) 603
SfuI TTCGAA 1 cut(s) 867
SinI GGWCC 1 cut(s) 4
SmiMI CAYNNNNRTG 2 cut(s) 82, 480
SmlI CTYRAG 3 cut(s) 555, 1139, 1474
SmoI CTYRAG 3 cut(s) 555, 1139, 1474
Sse9I AATT 6 cut(s) 45, 441, 900, 933, 1121, 1207
SsiI CCGC 5 cut(s) 131, 327, 727, 815, 1463
SspMI CTAG 3 cut(s) 302, 324, 1100
StyI CCWWGG 2 cut(s) 279, 1213
TaaI ACNGT 2 cut(s) 829, 1376
TaqI TCGA 8 cut(s) 174, 378, 583, 720, 861, 867, 1270, 1449
TasI AATT 6 cut(s) 45, 441, 900, 933, 1121, 1207
TatI WGTACW 1 cut(s) 1333
TauI GCSGC 3 cut(s) 134, 330, 818
TfiI GAWTC 4 cut(s) 176, 202, 274, 1381
Tru1I TTAA 5 cut(s) 159, 536, 846, 1119, 1358
Tru9I TTAA 5 cut(s) 159, 536, 846, 1119, 1358
TscAI CASTG 3 cut(s) 454, 567, 630
TseFI GTSAC 3 cut(s) 163, 734, 742
TseI GCWGC 7 cut(s) 67, 128, 602, 1349, 1364, 1420, 1465
Tsp45I GTSAC 3 cut(s) 163, 734, 742
TspDTI ATGAA 4 cut(s) 486, 793, 939, 1455
TspGWI ACGGA 1 cut(s) 6
TspRI CASTG 3 cut(s) 454, 567, 630
VpaK11BI GGWCC 1 cut(s) 4
VspI ATTAAT 1 cut(s) 1358
XapI RAATTY 3 cut(s) 441, 1121, 1207
XbaI TCTAGA 1 cut(s) 1099
XceI RCATGY 3 cut(s) 490, 580, 650
XmiI GTMKAC 1 cut(s) 1257
XspI CTAG 3 cut(s) 302, 324, 1100
Zsp2I ATGCAT 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.