MD09G1288500.v1.1

Copper-transporting ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0018103
Physical Location & Seq
Reverse (-)
36811225 .. 36811869
645 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1288500.v1.1.491

Sequence Viewer

Length: 645 bp
ATGAATTCTCTTGTCGGATTTGGATCCCTCGCAGCTTTTACAATTAGTGCAGTCTCGCTTCTCAACCTTGGGCTTCAATGGGATGCAGCGTTCTTCGATGAGCCGGTCATGCTTCTTGGTTTTGTGCTCCTAGGACGTTCTCTCGAGGAAAGGGCAAGGATCAAGGCATCTAGTGATATGAATGAACTCTTGTCACTGATGAACACACAAGCAAGACTTGTAATTGGTTCCCCGGAAAATGATTCCTTCAGCAACAGTGTACTTTTCTCTGATGCAATATGCGTTGAAGTCCCAACTGACAAGGTTCGGGTTGGAGACTCTGTGTTGGTTTTTCCAGGAGAAACCATACCTGTGGATGGAAGAGTTGTGGCTGGAAGAAGTGTTGTCGACGAATCCATGCTCACAGGAGAATCACTTCCTGTATTCAAGGAAAAAGACCTTACTGTCTCAGCTGGAACAATAAACTGGGATGGTCCTCTGCGGGTTGAAGCATCTTCAACTGGCTCCAATTCAATGATATTCAAGATCGTTCGCATGGTTGAGGATGCTCAAGGACATGAAGCACCCATACAAAGGCTTGCTGATTCAATAGCTGGACCTTTTGTATACAGTATAATGACTCTATCAGATCTCTCTCTCCTCTGA

Protein Analysis

215

Amino Acids

22.95

Weight (kDa)

4.42

Isoelectric Point (pI)

26.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E1-E2_ATPase PF00122 90 - 181 5.4e-28 P-type ATPase actuator domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 443
AccI GTMKAC 2 cut(s) 387, 606
AciI CCGC 1 cut(s) 481
AclWI GGATC 3 cut(s) 18, 31, 167
AcsI RAATTY 1 cut(s) 4
AcuI CTGAAG 1 cut(s) 232
AfaI GTAC 1 cut(s) 261
AfiI CCNNNNNNNGG 2 cut(s) 356, 573
AgsI TTSAA 8 cut(s) 77, 287, 427, 488, 498, 513, 523, 588
AjnI CCWGG 1 cut(s) 334
AloI GAACNNNNNNTCC 2 cut(s) 74, 106
AluBI AGCT 3 cut(s) 35, 452, 593
AluI AGCT 3 cut(s) 35, 452, 593
Alw21I GWGCWC 1 cut(s) 129
Alw26I GTCTC 3 cut(s) 58, 309, 451
AlwI GGATC 3 cut(s) 18, 31, 167
Ama87I CYCGRG 1 cut(s) 143
ApeKI GCWGC 2 cut(s) 32, 86
ApoI RAATTY 1 cut(s) 4
Asp700I GAANNNNTTC 1 cut(s) 414
AspA2I CCTAGG 1 cut(s) 130
AspS9I GGNCC 2 cut(s) 473, 596
AsuC2I CCSGG 1 cut(s) 233
AvaI CYCGRG 1 cut(s) 143
AvaII GGWCC 2 cut(s) 473, 596
AvrII CCTAGG 1 cut(s) 130
BamHI GGATCC 1 cut(s) 23
BarI GAAGNNNNNNTAC 2 cut(s) 552, 584
Bbv12I GWGCWC 1 cut(s) 129
BbvI GCAGC 2 cut(s) 44, 98
BccI CCATC 2 cut(s) 350, 464
BciT130I CCWGG 1 cut(s) 336
BcnI CCSGG 1 cut(s) 233
BcoDI GTCTC 3 cut(s) 58, 309, 451
BfaI CTAG 2 cut(s) 131, 171
BglII AGATCT 1 cut(s) 628
BisI GCNGC 2 cut(s) 33, 87
BlnI CCTAGG 1 cut(s) 130
BlsI GCNGC 2 cut(s) 34, 88
Bme1390I CCNGG 2 cut(s) 233, 336
Bme18I GGWCC 2 cut(s) 473, 596
BmeT110I CYCGRG 1 cut(s) 143
BmgT120I GGNCC 2 cut(s) 473, 596
BmiI GGNNCC 3 cut(s) 25, 229, 505
BmrFI CCNGG 2 cut(s) 233, 336
BmrI ACTGGG 1 cut(s) 475
BmsI GCATC 5 cut(s) 73, 176, 262, 500, 535
BmuI ACTGGG 1 cut(s) 475
BpuEI CTTGAG 1 cut(s) 534
BpuMI CCSGG 1 cut(s) 233
BsaBI GATNNNNATC 1 cut(s) 22
BsaJI CCNNGG 3 cut(s) 67, 130, 231
BsaXI ACNNNNNCTCC 2 cut(s) 306, 336
Bsc4I CCNNNNNNNGG 2 cut(s) 356, 573
Bse118I RCCGGY 1 cut(s) 103
Bse1I ACTGG 2 cut(s) 470, 505
Bse8I GATNNNNATC 1 cut(s) 22
BseBI CCWGG 1 cut(s) 336
BseDI CCNNGG 3 cut(s) 67, 130, 231
BseGI GGATG 4 cut(s) 88, 361, 475, 550
BseJI GATNNNNATC 1 cut(s) 22
BseLI CCNNNNNNNGG 2 cut(s) 356, 573
BseMII CTCAG 1 cut(s) 462
BseNI ACTGG 2 cut(s) 470, 505
BseRI GAGGAG 1 cut(s) 629
BseXI GCAGC 2 cut(s) 44, 98
BsgI GTGCAG 1 cut(s) 69
BsiHKAI GWGCWC 1 cut(s) 129
BsiHKCI CYCGRG 1 cut(s) 143
BsiSI CCGG 2 cut(s) 104, 233
BslFI GGGAC 1 cut(s) 275
BslI CCNNNNNNNGG 2 cut(s) 356, 573
BsmAI GTCTC 3 cut(s) 58, 309, 451
BsmFI GGGAC 1 cut(s) 275
BsoBI CYCGRG 1 cut(s) 143
Bsp1286I GDGCHC 1 cut(s) 129
Bsp143I GATC 4 cut(s) 23, 159, 525, 628
BspACI CCGC 1 cut(s) 481
BspCNI CTCAG 1 cut(s) 461
BspLI GGNNCC 3 cut(s) 25, 229, 505
BspPI GGATC 3 cut(s) 18, 31, 167
BsrFI RCCGGY 1 cut(s) 103
BsrI ACTGG 2 cut(s) 470, 505
BssAI RCCGGY 1 cut(s) 103
BssECI CCNNGG 3 cut(s) 67, 130, 231
BssMI GATC 4 cut(s) 23, 159, 525, 628
BssNAI GTATAC 1 cut(s) 607
BssT1I CCWWGG 2 cut(s) 67, 130
Bst1107I GTATAC 1 cut(s) 607
Bst2UI CCWGG 1 cut(s) 336
Bst4CI ACNGT 3 cut(s) 257, 445, 611
Bst6I CTCTTC 1 cut(s) 355
BstC8I GCNNGC 1 cut(s) 579
BstDEI CTNAG 1 cut(s) 448
BstF5I GGATG 4 cut(s) 88, 361, 475, 550
BstKTI GATC 4 cut(s) 26, 162, 528, 631
BstMAI GTCTC 3 cut(s) 58, 309, 451
BstMBI GATC 4 cut(s) 23, 159, 525, 628
BstMWI GCNNNNNNNGC 1 cut(s) 109
BstNI CCWGG 1 cut(s) 336
BstSCI CCNGG 2 cut(s) 231, 334
BstV1I GCAGC 2 cut(s) 44, 98
BstX2I RGATCY 2 cut(s) 23, 628
BstXI CCANNNNNNTGG 1 cut(s) 352
BstYI RGATCY 2 cut(s) 23, 628
BstZ17I GTATAC 1 cut(s) 607
BtsCI GGATG 4 cut(s) 88, 361, 475, 550
BtsIMutI CAGTG 2 cut(s) 194, 262
Cac8I GCNNGC 1 cut(s) 579
Cfr10I RCCGGY 1 cut(s) 103
Cfr13I GGNCC 2 cut(s) 473, 596
Csp6I GTAC 1 cut(s) 260
CviAII CATG 4 cut(s) 109, 397, 535, 557
CviJI RGCY 8 cut(s) 35, 73, 103, 371, 452, 504, 577, 593
CviKI_1 RGCY 8 cut(s) 35, 73, 103, 371, 452, 504, 577, 593
CviQI GTAC 1 cut(s) 260
DdeI CTNAG 1 cut(s) 448
DpnI GATC 4 cut(s) 25, 161, 527, 630
DpnII GATC 4 cut(s) 23, 159, 525, 628
DrdI GACNNNNNNGTC 1 cut(s) 443
DseDI GACNNNNNNGTC 1 cut(s) 443
Eam1104I CTCTTC 1 cut(s) 355
EarI CTCTTC 1 cut(s) 355
Eco130I CCWWGG 2 cut(s) 67, 130
Eco47I GGWCC 2 cut(s) 473, 596
Eco57I CTGAAG 1 cut(s) 232
Eco88I CYCGRG 1 cut(s) 143
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 1 cut(s) 334
EcoT14I CCWWGG 2 cut(s) 67, 130
ErhI CCWWGG 2 cut(s) 67, 130
FaeI CATG 4 cut(s) 112, 400, 538, 560
FalI AAGNNNNNCTT 2 cut(s) 201, 233
FaqI GGGAC 1 cut(s) 275
FatI CATG 4 cut(s) 108, 396, 534, 556
FauI CCCGC 1 cut(s) 474
FblI GTMKAC 2 cut(s) 387, 606
Fnu4HI GCNGC 2 cut(s) 33, 87
FokI GGATG 4 cut(s) 95, 368, 482, 557
Fsp4HI GCNGC 2 cut(s) 33, 87
FspBI CTAG 2 cut(s) 131, 171
GluI GCNGC 2 cut(s) 33, 87
HapII CCGG 2 cut(s) 104, 233
Hin1II CATG 4 cut(s) 112, 400, 538, 560
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HinfI GANTC 6 cut(s) 242, 317, 392, 410, 584, 619
HpaII CCGG 2 cut(s) 104, 233
Hpy166II GTNNAC 3 cut(s) 260, 388, 607
Hpy188I TCNGA 4 cut(s) 17, 271, 628, 644
Hpy188III TCNNGA 2 cut(s) 143, 523
Hpy8I GTNNAC 3 cut(s) 260, 388, 607
Hpy99I CGWCG 1 cut(s) 392
HpyAV CCTTC 1 cut(s) 256
HpyCH4III ACNGT 3 cut(s) 257, 445, 611
HpyCH4IV ACGT 1 cut(s) 136
HpyCH4V TGCA 3 cut(s) 50, 86, 275
HpyF10VI GCNNNNNNNGC 1 cut(s) 109
HpyF3I CTNAG 1 cut(s) 448
HpySE526I ACGT 1 cut(s) 136
Hsp92II CATG 4 cut(s) 112, 400, 538, 560
Kzo9I GATC 4 cut(s) 23, 159, 525, 628
LmnI GCTCC 2 cut(s) 132, 509
Lsp1109I GCAGC 2 cut(s) 44, 98
LweI GCATC 5 cut(s) 73, 176, 262, 500, 535
MaeI CTAG 2 cut(s) 131, 171
MaeII ACGT 1 cut(s) 136
MaeIII GTNAC 1 cut(s) 192
MalI GATC 4 cut(s) 25, 161, 527, 630
MboI GATC 4 cut(s) 23, 159, 525, 628
MboII GAAGA 4 cut(s) 85, 372, 387, 486
MflI RGATCY 2 cut(s) 23, 628
MhlI GDGCHC 1 cut(s) 129
MluCI AATT 4 cut(s) 4, 42, 222, 508
MlyI GAGTC 2 cut(s) 311, 613
MmeI TCCRAC 1 cut(s) 292
MnlI CCTC 4 cut(s) 38, 139, 486, 535
MroXI GAANNNNTTC 1 cut(s) 414
MslI CAYNNNNRTG 1 cut(s) 350
MspA1I CMGCKG 1 cut(s) 452
MspI CCGG 2 cut(s) 104, 233
MspR9I CCNGG 2 cut(s) 233, 336
MvaI CCWGG 1 cut(s) 336
MwoI GCNNNNNNNGC 1 cut(s) 109
NciI CCSGG 1 cut(s) 233
NdeII GATC 4 cut(s) 23, 159, 525, 628
NlaIII CATG 4 cut(s) 112, 400, 538, 560
NlaIV GGNNCC 3 cut(s) 25, 229, 505
NmuCI GTSAC 1 cut(s) 192
PaeR7I CTCGAG 1 cut(s) 143
PdmI GAANNNNTTC 1 cut(s) 414
PfeI GAWTC 4 cut(s) 242, 392, 410, 584
PfoI TCCNGGA 1 cut(s) 334
PkrI GCNGC 2 cut(s) 34, 88
PleI GAGTC 2 cut(s) 311, 613
PpsI GAGTC 2 cut(s) 311, 613
Psp6I CCWGG 1 cut(s) 334
PspGI CCWGG 1 cut(s) 334
PspN4I GGNNCC 3 cut(s) 25, 229, 505
PspPI GGNCC 2 cut(s) 473, 596
PsuI RGATCY 2 cut(s) 23, 628
PvuII CAGCTG 1 cut(s) 452
RsaI GTAC 1 cut(s) 261
RsaNI GTAC 1 cut(s) 260
RseI CAYNNNNRTG 1 cut(s) 350
SalI GTCGAC 1 cut(s) 386
SatI GCNGC 2 cut(s) 33, 87
Sau3AI GATC 4 cut(s) 23, 159, 525, 628
Sau96I GGNCC 2 cut(s) 473, 596
SchI GAGTC 2 cut(s) 311, 613
ScrFI CCNGG 2 cut(s) 233, 336
SduI GDGCHC 1 cut(s) 129
SetI ASST 9 cut(s) 37, 69, 139, 306, 352, 441, 454, 595, 601
SfaNI GCATC 5 cut(s) 73, 176, 262, 500, 535
Sfr274I CTCGAG 1 cut(s) 143
SinI GGWCC 2 cut(s) 473, 596
SlaI CTCGAG 1 cut(s) 143
SmiMI CAYNNNNRTG 1 cut(s) 350
SmlI CTYRAG 2 cut(s) 143, 549
SmoI CTYRAG 2 cut(s) 143, 549
Sse9I AATT 4 cut(s) 4, 42, 222, 508
SsiI CCGC 1 cut(s) 481
SspMI CTAG 2 cut(s) 131, 171
StyD4I CCNGG 2 cut(s) 231, 334
StyI CCWWGG 2 cut(s) 67, 130
TaaI ACNGT 3 cut(s) 257, 445, 611
TaiI ACGT 1 cut(s) 139
TaqI TCGA 3 cut(s) 96, 144, 387
TasI AATT 4 cut(s) 4, 42, 222, 508
TatI WGTACW 1 cut(s) 259
TfiI GAWTC 4 cut(s) 242, 392, 410, 584
TscAI CASTG 2 cut(s) 201, 262
TseFI GTSAC 1 cut(s) 192
TseI GCWGC 2 cut(s) 32, 86
Tsp45I GTSAC 1 cut(s) 192
TspDTI ATGAA 5 cut(s) 17, 194, 198, 215, 573
TspRI CASTG 2 cut(s) 201, 262
VpaK11BI GGWCC 2 cut(s) 473, 596
XapI RAATTY 1 cut(s) 4
XhoI CTCGAG 1 cut(s) 143
XmaJI CCTAGG 1 cut(s) 130
XmiI GTMKAC 2 cut(s) 387, 606
XmnI GAANNNNTTC 1 cut(s) 414
XspI CTAG 2 cut(s) 131, 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.