MD09G1293100.v1.1

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0015866
Physical Location & Seq
Forward (+)
37577144 .. 37578703
1560 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1293100.v1.1.491

Sequence Viewer

Length: 369 bp
ATGGGTCGAACAAAAACATTCAAGGACGAGTTTACATTTGATCAAAGGATTGAAGAATCATGGGACATGGTGACCAAATACCCTGATCGAGTCCCCGTAATTGTGGAAAAGTATGCGAGGAGCAATCTTCCTCAGATGGAAAAGAAAAAATACCTTGTCCCTCGAGACGTGTCCGTCGGCCAATTCATTTACGTTTTGAGCAGCAGACTTCACCTGGCCCCTGGAACAGCTCTCTTCGTTTTTGTGAAGGATACTTTGCCTCAAACAGCTAGTTTGATGGATTCTGTGTATGAATCGCTCAAGGACGAGGATGGATTTCTGTACCTGTGTTATAGCACTGAGAAGACCTTTGGCTTTCATCATCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04068 ko04136 ko04137 ko04138 ko04139 ko04140 ko04212 ko04371 ko04621 ko04727 ko05167 map04068 map04136 map04137 map04138 map04139 map04140 map04212 map04371 map04621 map04727 map05167
Pfam Domains
Protein Families
Atg8

Protein Analysis

123

Amino Acids

14.27

Weight (kDa)

6.28

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG8 PF02991 15 - 118 2.1e-42 Autophagy protein Atg8 ubiquitin like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015866)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15580
fragaria_vesca FvH4_1g26480
malus_domestica MD09G1293100.v1.1
prunus_persica Prupe.3G004300_v2.0.a1
pyrus_communis pycom09g19600
rosa_chinensis RchiOBHm_Chr3g0486541
rosa_laevigata RLG00000023078
rosa_multiflora Rmu_sc0003801.1_g000027 Rmu_sc0011408.1_g000005
rosa_roxburghii Rroxscaffold_6G00395810
rosa_rugosa Rorug03G0225000
rosa_samantha Rh3AG275000 Rh3CG309300 Rh3DG306100
rosa_wichuraiana Rw3G024510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 173
AcoI YGGCCR 1 cut(s) 178
AfaI GTAC 1 cut(s) 323
AflIII ACRYGT 1 cut(s) 168
AgsI TTSAA 2 cut(s) 22, 53
AjiI CACGTC 1 cut(s) 169
AjnI CCWGG 2 cut(s) 213, 220
AluBI AGCT 2 cut(s) 230, 269
AluI AGCT 2 cut(s) 230, 269
Alw26I GTCTC 1 cut(s) 159
Ama87I CYCGRG 1 cut(s) 162
AoxI GGCC 2 cut(s) 178, 216
ApeKI GCWGC 1 cut(s) 201
AspS9I GGNCC 1 cut(s) 217
AsuHPI GGTGA 2 cut(s) 82, 203
AvaI CYCGRG 1 cut(s) 162
BbsI GAAGAC 1 cut(s) 350
BbvI GCAGC 1 cut(s) 213
BccI CCATC 3 cut(s) 130, 271, 305
BciT130I CCWGG 2 cut(s) 215, 222
BciVI GTATCC 1 cut(s) 244
BclI TGATCA 1 cut(s) 40
BcoDI GTCTC 1 cut(s) 159
BfaI CTAG 1 cut(s) 270
BfuI GTATCC 1 cut(s) 244
BisI GCNGC 1 cut(s) 202
BlsI GCNGC 1 cut(s) 203
Bme1390I CCNGG 2 cut(s) 215, 222
BmeT110I CYCGRG 1 cut(s) 162
BmgBI CACGTC 1 cut(s) 169
BmgT120I GGNCC 1 cut(s) 217
BmiI GGNNCC 1 cut(s) 219
BmrFI CCNGG 2 cut(s) 215, 222
BpiI GAAGAC 1 cut(s) 350
BpuEI CTTGAG 1 cut(s) 284
BsaJI CCNNGG 1 cut(s) 220
BseBI CCWGG 2 cut(s) 215, 222
BseDI CCNNGG 1 cut(s) 220
BseGI GGATG 1 cut(s) 316
BseMII CTCAG 2 cut(s) 146, 330
BseRI GAGGAG 1 cut(s) 133
BseXI GCAGC 1 cut(s) 213
BshFI GGCC 2 cut(s) 180, 218
BsiHKCI CYCGRG 1 cut(s) 162
BslFI GGGAC 3 cut(s) 77, 77, 143
BsmAI GTCTC 1 cut(s) 159
BsmBI CGTCTC 1 cut(s) 159
BsmFI GGGAC 3 cut(s) 77, 77, 143
BsnI GGCC 2 cut(s) 180, 218
BsoBI CYCGRG 1 cut(s) 162
Bsp143I GATC 2 cut(s) 40, 85
BspANI GGCC 2 cut(s) 180, 218
BspCNI CTCAG 2 cut(s) 145, 331
BspLI GGNNCC 1 cut(s) 219
BssECI CCNNGG 1 cut(s) 220
BssMI GATC 2 cut(s) 40, 85
Bst2UI CCWGG 2 cut(s) 215, 222
Bst6I CTCTTC 1 cut(s) 239
BstDEI CTNAG 2 cut(s) 132, 339
BstEII GGTNACC 1 cut(s) 70
BstF5I GGATG 1 cut(s) 316
BstKTI GATC 2 cut(s) 43, 88
BstMAI GTCTC 1 cut(s) 159
BstMBI GATC 2 cut(s) 40, 85
BstNI CCWGG 2 cut(s) 215, 222
BstPI GGTNACC 1 cut(s) 70
BstSCI CCNGG 2 cut(s) 213, 220
BstV1I GCAGC 1 cut(s) 213
BstV2I GAAGAC 1 cut(s) 350
BsuI GTATCC 1 cut(s) 244
BsuRI GGCC 2 cut(s) 180, 218
BtrI CACGTC 1 cut(s) 169
BtsCI GGATG 1 cut(s) 316
BtsIMutI CAGTG 1 cut(s) 336
Cfr13I GGNCC 1 cut(s) 217
Csp6I GTAC 1 cut(s) 322
CviAII CATG 2 cut(s) 60, 67
CviJI RGCY 5 cut(s) 180, 218, 230, 269, 354
CviKI_1 RGCY 5 cut(s) 180, 218, 230, 269, 354
CviQI GTAC 1 cut(s) 322
DdeI CTNAG 2 cut(s) 132, 339
DpnI GATC 2 cut(s) 42, 87
DpnII GATC 2 cut(s) 40, 85
DrdI GACNNNNNNGTC 1 cut(s) 173
DseDI GACNNNNNNGTC 1 cut(s) 173
EaeI YGGCCR 1 cut(s) 178
Eam1104I CTCTTC 1 cut(s) 239
EarI CTCTTC 1 cut(s) 239
Eco88I CYCGRG 1 cut(s) 162
Eco91I GGTNACC 1 cut(s) 70
EcoO65I GGTNACC 1 cut(s) 70
EcoRII CCWGG 2 cut(s) 213, 220
Esp3I CGTCTC 1 cut(s) 159
FaeI CATG 2 cut(s) 63, 70
FaiI YATR 5 cut(s) 61, 68, 114, 291, 333
FaqI GGGAC 3 cut(s) 77, 77, 143
FatI CATG 2 cut(s) 59, 66
FbaI TGATCA 1 cut(s) 40
Fnu4HI GCNGC 1 cut(s) 202
FokI GGATG 1 cut(s) 323
Fsp4HI GCNGC 1 cut(s) 202
FspBI CTAG 1 cut(s) 270
GluI GCNGC 1 cut(s) 202
HaeIII GGCC 2 cut(s) 180, 218
Hin1II CATG 2 cut(s) 63, 70
HinfI GANTC 4 cut(s) 56, 90, 281, 293
HphI GGTGA 2 cut(s) 82, 203
Hpy166II GTNNAC 1 cut(s) 33
Hpy188I TCNGA 1 cut(s) 135
Hpy188III TCNNGA 1 cut(s) 164
Hpy8I GTNNAC 1 cut(s) 33
Hpy99I CGWCG 1 cut(s) 179
HpyAV CCTTC 1 cut(s) 241
HpyCH4IV ACGT 2 cut(s) 168, 192
HpyF3I CTNAG 2 cut(s) 132, 339
HpySE526I ACGT 2 cut(s) 168, 192
Hsp92II CATG 2 cut(s) 63, 70
Ksp22I TGATCA 1 cut(s) 40
Kzo9I GATC 2 cut(s) 40, 85
LmnI GCTCC 1 cut(s) 120
LpnPI CCDG 6 cut(s) 96, 200, 207, 227, 234, 338
Lsp1109I GCAGC 1 cut(s) 213
MaeI CTAG 1 cut(s) 270
MaeII ACGT 2 cut(s) 168, 192
MaeIII GTNAC 1 cut(s) 70
MalI GATC 2 cut(s) 42, 87
MboI GATC 2 cut(s) 40, 85
MboII GAAGA 4 cut(s) 65, 119, 226, 355
MluCI AATT 2 cut(s) 99, 182
MlyI GAGTC 1 cut(s) 99
MnlI CCTC 5 cut(s) 111, 141, 171, 270, 301
MspR9I CCNGG 2 cut(s) 215, 222
MvaI CCWGG 2 cut(s) 215, 222
NdeII GATC 2 cut(s) 40, 85
NlaIII CATG 2 cut(s) 63, 70
NlaIV GGNNCC 1 cut(s) 219
NmuCI GTSAC 1 cut(s) 70
PaeR7I CTCGAG 1 cut(s) 162
PfeI GAWTC 3 cut(s) 56, 281, 293
PkrI GCNGC 1 cut(s) 203
PleI GAGTC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 98
Psp6I CCWGG 2 cut(s) 213, 220
PspEI GGTNACC 1 cut(s) 70
PspGI CCWGG 2 cut(s) 213, 220
PspN4I GGNNCC 1 cut(s) 219
PspPI GGNCC 1 cut(s) 217
RsaI GTAC 1 cut(s) 323
RsaNI GTAC 1 cut(s) 322
SatI GCNGC 1 cut(s) 202
Sau3AI GATC 2 cut(s) 40, 85
Sau96I GGNCC 1 cut(s) 217
SchI GAGTC 1 cut(s) 99
ScrFI CCNGG 2 cut(s) 215, 222
SetI ASST 8 cut(s) 156, 171, 195, 216, 232, 271, 327, 350
Sfr274I CTCGAG 1 cut(s) 162
SlaI CTCGAG 1 cut(s) 162
SmlI CTYRAG 2 cut(s) 162, 299
SmoI CTYRAG 2 cut(s) 162, 299
Sse9I AATT 2 cut(s) 99, 182
SspMI CTAG 1 cut(s) 270
StyD4I CCNGG 2 cut(s) 213, 220
TaiI ACGT 2 cut(s) 171, 195
TaqI TCGA 3 cut(s) 7, 88, 163
TasI AATT 2 cut(s) 99, 182
TfiI GAWTC 3 cut(s) 56, 281, 293
TscAI CASTG 1 cut(s) 343
TseFI GTSAC 1 cut(s) 70
TseI GCWGC 1 cut(s) 201
Tsp45I GTSAC 1 cut(s) 70
TspDTI ATGAA 3 cut(s) 175, 306, 347
TspGWI ACGGA 1 cut(s) 163
TspRI CASTG 1 cut(s) 343
XhoI CTCGAG 1 cut(s) 162
XspI CTAG 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.