pycom09g19600

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0015866
Physical Location & Seq
Reverse (-)
20462935 .. 20464163
1229 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g19600.1

Sequence Viewer

Length: 528 bp
ATGGGCCTCGCCGGGTTGGACCCAAATATTCGTCATCTCGTCTGCGGTTGCATCGCGTTTGCGGCACTCTTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTTCCTCCTATCTCATCAGAGAGTCAGAGTCGGAGAGACAGAGCGACAGAAAGCGATGGGTCGAACAACAACATTCAAGGACGAGTTTACATTTGATCAAAGGATTGAAGAGTCACGGGACATGGTGGCCAAATACCCTGATCGAGTCCCCGTCATTGTGGAAAAGTATGCGAGGAGCAATCTTCCTCAGATGGAAAAGAAAAAATACCTTGTCCCTCGAGACATGTCTGTCGGCCAATTCATTTACGTTTTGAGCAGCAGACTTCACCTGGCCCCCGGAACAGCTCTCTTCGTTTTTGTGAAGGATACTTTGCCTCAAACAGCTAGTTTGATGGATTCTGTATATGAATCGTTCAAGGACGAGGATGGATTTCTGTACCTGTGTTATAGTACTGAGAAAACCTTTGGCTTTCATCATCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04068 ko04136 ko04137 ko04138 ko04139 ko04140 ko04212 ko04371 ko04621 ko04727 ko05167 map04068 map04136 map04137 map04138 map04139 map04140 map04212 map04371 map04621 map04727 map05167
Pfam Domains
Protein Families
Atg8

Protein Analysis

176

Amino Acids

19.83

Weight (kDa)

7.74

Isoelectric Point (pI)

50.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG8 PF02991 68 - 171 5.1e-43 Autophagy protein Atg8 ubiquitin like
APG12 PF04110 92 - 171 9.4e-06 Ubiquitin-like autophagy protein Apg12
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015866)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15580
fragaria_vesca FvH4_1g26480
malus_domestica MD09G1293100.v1.1
prunus_persica Prupe.3G004300_v2.0.a1
pyrus_communis pycom09g19600
rosa_chinensis RchiOBHm_Chr3g0486541
rosa_laevigata RLG00000023078
rosa_multiflora Rmu_sc0003801.1_g000027 Rmu_sc0011408.1_g000005
rosa_roxburghii Rroxscaffold_6G00395810
rosa_rugosa Rorug03G0225000
rosa_samantha Rh3AG275000 Rh3CG309300 Rh3DG306100
rosa_wichuraiana Rw3G024510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 332
AccII CGCG 1 cut(s) 56
AciI CCGC 2 cut(s) 45, 62
AcoI YGGCCR 2 cut(s) 231, 337
AfaI GTAC 2 cut(s) 482, 496
AflIII ACRYGT 1 cut(s) 327
AgsI TTSAA 3 cut(s) 181, 212, 460
AjnI CCWGG 1 cut(s) 372
AluBI AGCT 2 cut(s) 389, 428
AluI AGCT 2 cut(s) 389, 428
Alw26I GTCTC 2 cut(s) 134, 318
Ama87I CYCGRG 1 cut(s) 321
AoxI GGCC 4 cut(s) 4, 231, 337, 375
ApeKI GCWGC 1 cut(s) 360
AspS9I GGNCC 3 cut(s) 4, 19, 376
AsuC2I CCSGG 2 cut(s) 13, 381
AsuHPI GGTGA 1 cut(s) 362
AvaI CYCGRG 1 cut(s) 321
AvaII GGWCC 1 cut(s) 19
BalI TGGCCA 1 cut(s) 233
BbvI GCAGC 1 cut(s) 372
BccI CCATC 4 cut(s) 154, 289, 430, 464
BciT130I CCWGG 1 cut(s) 374
BciVI GTATCC 1 cut(s) 403
BclI TGATCA 1 cut(s) 199
BcnI CCSGG 2 cut(s) 13, 381
BcoDI GTCTC 2 cut(s) 134, 318
BfaI CTAG 1 cut(s) 429
BfuI GTATCC 1 cut(s) 403
BisI GCNGC 2 cut(s) 63, 361
BlsI GCNGC 2 cut(s) 64, 362
BmcAI AGTACT 1 cut(s) 496
Bme1390I CCNGG 3 cut(s) 13, 374, 381
Bme18I GGWCC 1 cut(s) 19
BmeT110I CYCGRG 1 cut(s) 321
BmgT120I GGNCC 3 cut(s) 4, 19, 376
BmiI GGNNCC 2 cut(s) 21, 378
BmrFI CCNGG 3 cut(s) 13, 374, 381
BmsI GCATC 1 cut(s) 60
BpuMI CCSGG 2 cut(s) 13, 381
BsaJI CCNNGG 1 cut(s) 379
BseBI CCWGG 1 cut(s) 374
BseDI CCNNGG 1 cut(s) 379
BseGI GGATG 1 cut(s) 475
BseMII CTCAG 2 cut(s) 305, 489
BseRI GAGGAG 1 cut(s) 292
BseXI GCAGC 1 cut(s) 372
Bsh1236I CGCG 1 cut(s) 56
BshFI GGCC 4 cut(s) 6, 233, 339, 377
BsiHKCI CYCGRG 1 cut(s) 321
BsiSI CCGG 2 cut(s) 12, 381
BslFI GGGAC 3 cut(s) 236, 236, 302
BsmAI GTCTC 2 cut(s) 134, 318
BsmFI GGGAC 3 cut(s) 236, 236, 302
BsnI GGCC 4 cut(s) 6, 233, 339, 377
BsoBI CYCGRG 1 cut(s) 321
Bsp143I GATC 2 cut(s) 199, 244
BspACI CCGC 2 cut(s) 45, 62
BspANI GGCC 4 cut(s) 6, 233, 339, 377
BspCNI CTCAG 2 cut(s) 304, 490
BspFNI CGCG 1 cut(s) 56
BspLI GGNNCC 2 cut(s) 21, 378
BssECI CCNNGG 1 cut(s) 379
BssMI GATC 2 cut(s) 199, 244
Bst2UI CCWGG 1 cut(s) 374
Bst6I CTCTTC 2 cut(s) 207, 398
BstDEI CTNAG 2 cut(s) 291, 498
BstF5I GGATG 1 cut(s) 475
BstFNI CGCG 1 cut(s) 56
BstKTI GATC 2 cut(s) 202, 247
BstMAI GTCTC 2 cut(s) 134, 318
BstMBI GATC 2 cut(s) 199, 244
BstMWI GCNNNNNNNGC 1 cut(s) 62
BstNI CCWGG 1 cut(s) 374
BstNSI RCATGY 1 cut(s) 331
BstSCI CCNGG 3 cut(s) 11, 372, 379
BstUI CGCG 1 cut(s) 56
BstV1I GCAGC 1 cut(s) 372
BsuI GTATCC 1 cut(s) 403
BsuRI GGCC 4 cut(s) 6, 233, 339, 377
BtgZI GCGATG 2 cut(s) 37, 173
BtsCI GGATG 1 cut(s) 475
Cfr13I GGNCC 3 cut(s) 4, 19, 376
Csp6I GTAC 2 cut(s) 481, 495
CviAII CATG 2 cut(s) 226, 328
CviJI RGCY 7 cut(s) 6, 233, 339, 377, 389, 428, 513
CviKI_1 RGCY 7 cut(s) 6, 233, 339, 377, 389, 428, 513
CviQI GTAC 2 cut(s) 481, 495
DdeI CTNAG 2 cut(s) 291, 498
DpnI GATC 2 cut(s) 201, 246
DpnII GATC 2 cut(s) 199, 244
DrdI GACNNNNNNGTC 1 cut(s) 332
DseDI GACNNNNNNGTC 1 cut(s) 332
EaeI YGGCCR 2 cut(s) 231, 337
Eam1104I CTCTTC 2 cut(s) 207, 398
EarI CTCTTC 2 cut(s) 207, 398
Eco47I GGWCC 1 cut(s) 19
Eco88I CYCGRG 1 cut(s) 321
EcoRII CCWGG 1 cut(s) 372
FaeI CATG 2 cut(s) 229, 331
FaiI YATR 6 cut(s) 227, 273, 329, 448, 450, 492
FaqI GGGAC 3 cut(s) 236, 236, 302
FatI CATG 2 cut(s) 225, 327
FbaI TGATCA 1 cut(s) 199
Fnu4HI GCNGC 2 cut(s) 63, 361
FokI GGATG 1 cut(s) 482
Fsp4HI GCNGC 2 cut(s) 63, 361
FspBI CTAG 1 cut(s) 429
GluI GCNGC 2 cut(s) 63, 361
HaeIII GGCC 4 cut(s) 6, 233, 339, 377
HapII CCGG 2 cut(s) 12, 381
Hin1II CATG 2 cut(s) 229, 331
HinfI GANTC 6 cut(s) 126, 132, 215, 249, 440, 452
HpaII CCGG 2 cut(s) 12, 381
HphI GGTGA 1 cut(s) 362
Hpy166II GTNNAC 1 cut(s) 192
Hpy188I TCNGA 4 cut(s) 123, 131, 137, 294
Hpy188III TCNNGA 1 cut(s) 323
Hpy8I GTNNAC 1 cut(s) 192
HpyAV CCTTC 2 cut(s) 115, 400
HpyCH4IV ACGT 1 cut(s) 351
HpyCH4V TGCA 1 cut(s) 51
HpyF10VI GCNNNNNNNGC 1 cut(s) 62
HpyF3I CTNAG 2 cut(s) 291, 498
HpySE526I ACGT 1 cut(s) 351
Hsp92II CATG 2 cut(s) 229, 331
Ksp22I TGATCA 1 cut(s) 199
Kzo9I GATC 2 cut(s) 199, 244
LmnI GCTCC 1 cut(s) 279
LpnPI CCDG 6 cut(s) 25, 255, 359, 386, 394, 497
Lsp1109I GCAGC 1 cut(s) 372
LweI GCATC 1 cut(s) 60
MaeI CTAG 1 cut(s) 429
MaeII ACGT 1 cut(s) 351
MaeIII GTNAC 1 cut(s) 216
MalI GATC 2 cut(s) 201, 246
MboI GATC 2 cut(s) 199, 244
MlsI TGGCCA 1 cut(s) 233
MluCI AATT 1 cut(s) 341
MluNI TGGCCA 1 cut(s) 233
MlyI GAGTC 4 cut(s) 135, 141, 224, 258
MmeI TCCRAC 1 cut(s) 115
MnlI CCTC 7 cut(s) 17, 119, 270, 300, 330, 429, 460
Mox20I TGGCCA 1 cut(s) 233
MscI TGGCCA 1 cut(s) 233
Msp20I TGGCCA 1 cut(s) 233
MspI CCGG 2 cut(s) 12, 381
MspR9I CCNGG 3 cut(s) 13, 374, 381
MvaI CCWGG 1 cut(s) 374
MvnI CGCG 1 cut(s) 56
MwoI GCNNNNNNNGC 1 cut(s) 62
NciI CCSGG 2 cut(s) 13, 381
NdeII GATC 2 cut(s) 199, 244
NlaIII CATG 2 cut(s) 229, 331
NlaIV GGNNCC 2 cut(s) 21, 378
NmuCI GTSAC 1 cut(s) 216
NspI RCATGY 1 cut(s) 331
PaeR7I CTCGAG 1 cut(s) 321
PciI ACATGT 1 cut(s) 327
PfeI GAWTC 2 cut(s) 440, 452
PkrI GCNGC 2 cut(s) 64, 362
PleI GAGTC 4 cut(s) 134, 140, 223, 257
PpsI GAGTC 4 cut(s) 134, 140, 223, 257
PscI ACATGT 1 cut(s) 327
Psp6I CCWGG 1 cut(s) 372
PspGI CCWGG 1 cut(s) 372
PspN4I GGNNCC 2 cut(s) 21, 378
PspPI GGNCC 3 cut(s) 4, 19, 376
RsaI GTAC 2 cut(s) 482, 496
RsaNI GTAC 2 cut(s) 481, 495
SatI GCNGC 2 cut(s) 63, 361
Sau3AI GATC 2 cut(s) 199, 244
Sau96I GGNCC 3 cut(s) 4, 19, 376
ScaI AGTACT 1 cut(s) 496
SchI GAGTC 4 cut(s) 135, 141, 224, 258
ScrFI CCNGG 3 cut(s) 13, 374, 381
SetI ASST 7 cut(s) 315, 354, 375, 391, 430, 486, 509
SfaNI GCATC 1 cut(s) 60
Sfr274I CTCGAG 1 cut(s) 321
SinI GGWCC 1 cut(s) 19
SlaI CTCGAG 1 cut(s) 321
SmlI CTYRAG 1 cut(s) 321
SmoI CTYRAG 1 cut(s) 321
Sse9I AATT 1 cut(s) 341
SsiI CCGC 2 cut(s) 45, 62
SspI AATATT 1 cut(s) 28
SspMI CTAG 1 cut(s) 429
StyD4I CCNGG 3 cut(s) 11, 372, 379
TaiI ACGT 1 cut(s) 354
TaqI TCGA 3 cut(s) 166, 247, 322
TasI AATT 1 cut(s) 341
TatI WGTACW 1 cut(s) 494
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 2 cut(s) 440, 452
TseFI GTSAC 1 cut(s) 216
TseI GCWGC 1 cut(s) 360
Tsp45I GTSAC 1 cut(s) 216
TspDTI ATGAA 3 cut(s) 334, 465, 506
VpaK11BI GGWCC 1 cut(s) 19
XceI RCATGY 1 cut(s) 331
XhoI CTCGAG 1 cut(s) 321
XspI CTAG 1 cut(s) 429
ZrmI AGTACT 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.