Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0014380

Physical Location & Seq

Genomic Coordinates Forward (+)

5380181 .. 5381911

Gene Structure 1731 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1041300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1731 bp

ATGGCCAGCTTGGAGTCCTTGTTACAGAAATGCACCTCCCTCACGAGCATCAAACAGCTCCAAGCCCACCTTCTAACCAGTGGCAAATTCCAATTCTACCCCTCCCTCACCACCATACTCCTCGAGCGCTGCGCCCTCTCCCCCGTCGCCAACCTCCCTTACGCGACAGCCCTCTTCTGCCACCTCCAAAACCCTTCCACCAATCAATGGAACGCCGTCGTTCGAGGCCTCGCCCAGAGCCTCCAACCCACCCAAGCCATTGCATGGTACAAGACCATGTCCCAAGCTTCCCAGAAAATCGACGCCCTCACGTGCTCCTTCGCTCTAAAGGCGTGCGCACGTGCATTGGCTTTCTCTGAGGCAATGCAGATTCACTCTCAGATTGTACAGTTCGGATTTGGAGCCGACGTGCTGCTGCGAACTACTTTGCTGGACGTTTATGCGAAAGTGGGTGATTTGGGGTTTGCACAGAAAGTGTTTGATGAAATGGGTCAAAGAGATATTGCTTGTTGGAATGCTTTAATTTCTGGGTTGGCTCAGGGGAATCGACCCACTGAAGCTATAGCTTTGTTTCAGAGAATGAGCGAGGAAGAGGGTTTGAAGCCTAATGAAGTCACCGTTCTTGGTGCGCTCTCTGCCTGTTCGCAATTGGGTGCACTCAAAAGAGGAGAGAAAATACATGCCTATATAATGGATGAGAAGCTTGATATGCACGTCATTGTTTGCAATGCGGTTATTGATATGTACGCGAAATGCGGCTTTGAGGACAAAGCATATTGGGTGTTTCAGAATATGAAATGTGGGAAGAATCTAATAACGTGGAATACGATGATTATGGCGTTTGCCATGCATGGCAATGGTGACAAAGCACTTGAGCTTTTCGGACAGATGGATAAAAGTGCGGTGTGCCCAGATGCAGTGTCGTATCTTGCTGCTTTGTGTGCCTGCAACCATGCCGGACTGGTGGCAGATGGGGTCAGGTTATTTAATTCGATGGCCGGGCATGGGGTTGCCCCTAATGTTAAGCATTATGGAACTGTGGTTGATTTGCTGGGAAGAGCTGGGCGTATTCAAGAGGCTTACGAGATTATAAATTATATGCCTATGTTTCCTGATGTGGTACTCTGGCAAACTTTACTTGGTGCTAGCAAGACTTATAGGAATGTAGAGATGGCAGAACTGGCGTCTCGGAAACTGATTGAGTTGGGGTCTAAGGGTTGTGGTGATTTCGTATTGTTATCAAATGTTTACGCGGCTCATGAGAGATGGGATGATGTAGGCAGGGTGAGGGAGGCCATGAAGAAAAGGGATGTGAAGAAGATACCGGGTTTTGGGTACATAGAAGTGGAAGGTGTGATACACAAGTTCGTTAATGGTGATCAAAGCCATACTAATTGGCGTGAGATTTATGCAAAGCTGGATGAGATCATGTTCAGGATTAAAGCCTATGGATATGCGGCCAAGACTAATAATGTGCTGCATGATATAGGGGAGGAGGAAAAAGAGAATGCATTGAGCTACCACTGCGAGAAATTGGCTGTGGCGTTTGGTTTGATTAGTTCCAATGAGGGGACACCAATTCAAGTGATTAAGAACCTAAGAATATGTGAGGATTGCCATGTTGTGATCAAACTTATTTCGAAGGTTTATAACAGGGAAATAATTGTGAGGGATCGAGCCCGGTTTCACAGATTTAAAGAAGGATTATGCTCTTGCAGAGATTATTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000959 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016071 GO:0016556 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0080156 GO:0090304 GO:0090305 GO:0097159 GO:1900864 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2 ThiC_Rad_SAM

Protein Analysis

577

Amino Acids

64.21

Weight (kDa)

7.99

Isoelectric Point (pI)

36.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	167 - 214	1.9e-07	PPR repeat family
PPR_2	PF13041	270 - 316	1.1e-09	PPR repeat family
PPR	PF01535	272 - 299	1.2e-07	PPR repeat
E_motif	PF20431	386 - 448	3.1e-19	E motif
DYW_deaminase	PF14432	484 - 576	4.5e-39	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014380)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G34160 AT1G34160
fragaria_vesca	FvH4_2g12730
malus_domestica	MD05G1037500.v1.1 MD10G1041300.v1.1
prunus_persica	Prupe.8G045500_v2.0.a1 Prupe.8G045500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0273501
rosa_laevigata	RLG00000013612
rosa_multiflora	Rmu_co8087438.1_g000001 Rmu_sc0026438.1_g000001
rosa_roxburghii	Rroxscaffold_7G00195290
rosa_rugosa	Rorug06G0075900
rosa_samantha	Rh6AG190600 Rh6BG194100 Rh6DG185200
rosa_wichuraiana	Rw6G016680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1091, 1650
Acc16I	TGCGCA	1 cut(s)	337
AccB7I	CCANNNNNTGG	2 cut(s)	207, 264
AccII	CGCG	3 cut(s)	164, 749, 1253
AciI	CCGC	5 cut(s)	731, 756, 902, 1253, 1457
AclWI	GGATC	1 cut(s)	1680
AcoI	YGGCCR	3 cut(s)	3, 996, 1458
AcsI	RAATTY	1 cut(s)	86
AcuI	CTGAAG	1 cut(s)	576
AcvI	CACGTG	2 cut(s)	312, 341
AcyI	GRCGYC	2 cut(s)	303, 1184
AfaI	GTAC	5 cut(s)	269, 387, 746, 1122, 1337
AfeI	AGCGCT	1 cut(s)	128
AfiI	CCNNNNNNNGG	5 cut(s)	207, 264, 1005, 1331, 1569
AgsI	TTSAA	3 cut(s)	601, 1073, 1583
AjiI	CACGTC	2 cut(s)	409, 715
AjuI	GAANNNNNNNTTGG	2 cut(s)	54, 86
Alw21I	GWGCWC	2 cut(s)	317, 658
Alw26I	GTCTC	1 cut(s)	1191
Alw44I	GTGCAC	1 cut(s)	654
AlwI	GGATC	1 cut(s)	1680
Ama87I	CYCGRG	1 cut(s)	122
Aor51HI	AGCGCT	1 cut(s)	128
AoxI	GGCC	5 cut(s)	3, 226, 996, 1293, 1458
ApaLI	GTGCAC	1 cut(s)	654
ApeKI	GCWGC	5 cut(s)	129, 412, 415, 932, 1477
ApoI	RAATTY	1 cut(s)	86
AspLEI	GCGC	4 cut(s)	129, 134, 338, 631
AsuC2I	CCSGG	3 cut(s)	1000, 1326, 1681
AsuHPI	GGTGA	7 cut(s)	100, 464, 607, 872, 1235, 1297, 1388
AsuII	TTCGAA	1 cut(s)	1640
AsuNHI	GCTAGC	1 cut(s)	1145
AvaI	CYCGRG	1 cut(s)	122
BaeGI	GKGCMC	2 cut(s)	658, 911
BaeI	ACNNNNGTAYC	2 cut(s)	1349, 1382
BalI	TGGCCA	1 cut(s)	5
BanII	GRGCYC	1 cut(s)	1681
BarI	GAAGNNNNNNTAC	2 cut(s)	1341, 1373
BauI	CACGAG	1 cut(s)	43
BbrPI	CACGTG	2 cut(s)	312, 341
Bbv12I	GWGCWC	2 cut(s)	317, 658
BbvI	GCAGC	5 cut(s)	116, 399, 402, 919, 1464
BccI	CCATC	5 cut(s)	883, 965, 988, 1165, 1260
BceAI	ACGGC	1 cut(s)	200
BclI	TGATCA	2 cut(s)	1378, 1626
BcnI	CCSGG	3 cut(s)	1000, 1326, 1681
BcoDI	GTCTC	1 cut(s)	1191
BfaI	CTAG	1 cut(s)	1146
BfmI	CTRYAG	1 cut(s)	561
BfoI	RGCGCY	1 cut(s)	130
BisI	GCNGC	8 cut(s)	130, 413, 416, 757, 933, 1254, 1458, 1478
BlsI	GCNGC	8 cut(s)	131, 414, 417, 758, 934, 1255, 1459, 1479
Bme1390I	CCNGG	3 cut(s)	1000, 1326, 1681
BmeT110I	CYCGRG	1 cut(s)	122
BmgBI	CACGTC	2 cut(s)	409, 715
BmiI	GGNNCC	1 cut(s)	403
BmrFI	CCNGG	3 cut(s)	1000, 1326, 1681
BmsI	GCATC	2 cut(s)	57, 904
BmtI	GCTAGC	1 cut(s)	1149
Bpu10I	CCTNAGC	1 cut(s)	537
Bpu14I	TTCGAA	1 cut(s)	1640
BpuEI	CTTGAG	1 cut(s)	893
BpuMI	CCSGG	3 cut(s)	1000, 1326, 1681
BsaAI	YACGTR	2 cut(s)	312, 341
BsaHI	GRCGYC	2 cut(s)	303, 1184
BsaXI	ACNNNNNCTCC	4 cut(s)	5, 35, 393, 423
Bsc4I	CCNNNNNNNGG	5 cut(s)	207, 264, 1005, 1331, 1569
Bse1I	ACTGG	3 cut(s)	78, 966, 1185
Bse3DI	GCAATG	4 cut(s)	258, 369, 733, 862
BseGI	GGATG	4 cut(s)	700, 1276, 1315, 1426
BseLI	CCNNNNNNNGG	5 cut(s)	207, 264, 1005, 1331, 1569
BseMI	GCAATG	4 cut(s)	258, 369, 733, 862
BseMII	CTCAG	3 cut(s)	348, 392, 551
BseNI	ACTGG	3 cut(s)	78, 966, 1185
BseRI	GAGGAG	3 cut(s)	110, 681, 1508
BseSI	GKGCMC	2 cut(s)	658, 911
BseXI	GCAGC	5 cut(s)	116, 399, 402, 919, 1464
BseYI	CCCAGC	2 cut(s)	1051, 1061
Bsh1236I	CGCG	3 cut(s)	164, 749, 1253
BshFI	GGCC	5 cut(s)	5, 228, 998, 1295, 1460
BsiHKAI	GWGCWC	2 cut(s)	317, 658
BsiHKCI	CYCGRG	1 cut(s)	122
BsiSI	CCGG	4 cut(s)	957, 999, 1325, 1681
BslFI	GGGAC	2 cut(s)	265, 1585
BslI	CCNNNNNNNGG	5 cut(s)	207, 264, 1005, 1331, 1569
BsmAI	GTCTC	1 cut(s)	1191
BsmBI	CGTCTC	1 cut(s)	1191
BsmFI	GGGAC	2 cut(s)	265, 1585
BsmI	GAATGC	2 cut(s)	520, 1513
BsnI	GGCC	5 cut(s)	5, 228, 998, 1295, 1460
BsoBI	CYCGRG	1 cut(s)	122
Bsp119I	TTCGAA	1 cut(s)	1640
Bsp1286I	GDGCHC	4 cut(s)	317, 658, 911, 1681
Bsp1407I	TGTACA	1 cut(s)	385
Bsp143I	GATC	4 cut(s)	1378, 1425, 1626, 1672
BspACI	CCGC	5 cut(s)	731, 756, 902, 1253, 1457
BspANI	GGCC	5 cut(s)	5, 228, 998, 1295, 1460
BspCNI	CTCAG	3 cut(s)	349, 391, 550
BspFNI	CGCG	3 cut(s)	164, 749, 1253
BspHI	TCATGA	1 cut(s)	1258
BspLI	GGNNCC	1 cut(s)	403
BspOI	GCTAGC	1 cut(s)	1149
BspPI	GGATC	1 cut(s)	1680
BspQI	GCTCTTC	1 cut(s)	1051
BspT104I	TTCGAA	1 cut(s)	1640
BsrDI	GCAATG	4 cut(s)	258, 369, 733, 862
BsrGI	TGTACA	1 cut(s)	385
BsrI	ACTGG	3 cut(s)	78, 966, 1185
BssMI	GATC	4 cut(s)	1378, 1425, 1626, 1672
BssNI	GRCGYC	2 cut(s)	303, 1184
BssSI	CACGAG	1 cut(s)	43
Bst2BI	CACGAG	1 cut(s)	43
Bst4CI	ACNGT	3 cut(s)	390, 619, 1039
Bst6I	CTCTTC	3 cut(s)	179, 585, 1051
BstACI	GRCGYC	2 cut(s)	303, 1184
BstAUI	TGTACA	1 cut(s)	385
BstBAI	YACGTR	2 cut(s)	312, 341
BstBI	TTCGAA	1 cut(s)	1640
BstC8I	GCNNGC	4 cut(s)	7, 334, 946, 1147
BstDEI	CTNAG	5 cut(s)	357, 378, 537, 1212, 1598
BstF5I	GGATG	4 cut(s)	700, 1276, 1315, 1426
BstFNI	CGCG	3 cut(s)	164, 749, 1253
BstH2I	RGCGCY	1 cut(s)	130
BstHHI	GCGC	4 cut(s)	129, 134, 338, 631
BstKTI	GATC	4 cut(s)	1381, 1428, 1629, 1675
BstMAI	GTCTC	1 cut(s)	1191
BstMBI	GATC	4 cut(s)	1378, 1425, 1626, 1672
BstMWI	GCNNNNNNNGC	6 cut(s)	329, 635, 709, 941, 1181, 1524
BstNSI	RCATGY	1 cut(s)	683
BstSCI	CCNGG	3 cut(s)	998, 1324, 1679
BstSFI	CTRYAG	1 cut(s)	561
BstSLI	GKGCMC	2 cut(s)	658, 911
BstUI	CGCG	3 cut(s)	164, 749, 1253
BstV1I	GCAGC	5 cut(s)	116, 399, 402, 919, 1464
BsuRI	GGCC	5 cut(s)	5, 228, 998, 1295, 1460
BtrI	CACGTC	2 cut(s)	409, 715
BtsCI	GGATG	4 cut(s)	700, 1276, 1315, 1426
BtsI	GCAGTG	2 cut(s)	924, 1522
BtsIMutI	CAGTG	4 cut(s)	85, 552, 924, 1522
Cac8I	GCNNGC	4 cut(s)	7, 334, 946, 1147
CciI	TCATGA	1 cut(s)	1258
CfoI	GCGC	4 cut(s)	129, 134, 338, 631
CseI	GACGC	2 cut(s)	311, 1173
Csp6I	GTAC	5 cut(s)	268, 386, 745, 1121, 1336
CviQI	GTAC	5 cut(s)	268, 386, 745, 1121, 1336
DdeI	CTNAG	5 cut(s)	357, 378, 537, 1212, 1598
DpnI	GATC	4 cut(s)	1380, 1427, 1628, 1674
DpnII	GATC	4 cut(s)	1378, 1425, 1626, 1672
DraI	TTTAAA	1 cut(s)	1696
EaeI	YGGCCR	3 cut(s)	3, 996, 1458
Eam1104I	CTCTTC	3 cut(s)	179, 585, 1051
EarI	CTCTTC	3 cut(s)	179, 585, 1051
Eco147I	AGGCCT	1 cut(s)	228
Eco24I	GRGCYC	1 cut(s)	1681
Eco47III	AGCGCT	1 cut(s)	128
Eco57I	CTGAAG	1 cut(s)	576
Eco72I	CACGTG	2 cut(s)	312, 341
Eco88I	CYCGRG	1 cut(s)	122
EcoT22I	ATGCAT	2 cut(s)	852, 1513
EcoT38I	GRGCYC	1 cut(s)	1681
Esp3I	CGTCTC	1 cut(s)	1191
FalI	AAGNNNNNCTT	2 cut(s)	54, 86
FaqI	GGGAC	2 cut(s)	265, 1585
FbaI	TGATCA	2 cut(s)	1378, 1626
Fnu4HI	GCNGC	8 cut(s)	130, 413, 416, 757, 933, 1254, 1458, 1478
FokI	GGATG	4 cut(s)	707, 1283, 1322, 1433
FriOI	GRGCYC	1 cut(s)	1681
Fsp4HI	GCNGC	8 cut(s)	130, 413, 416, 757, 933, 1254, 1458, 1478
FspAI	RTGCGCAY	1 cut(s)	337
FspBI	CTAG	1 cut(s)	1146
FspI	TGCGCA	1 cut(s)	337
GlaI	GCGC	4 cut(s)	128, 133, 337, 630
GluI	GCNGC	8 cut(s)	130, 413, 416, 757, 933, 1254, 1458, 1478
GsaI	CCCAGC	2 cut(s)	1055, 1065
HaeII	RGCGCY	1 cut(s)	130
HaeIII	GGCC	5 cut(s)	5, 228, 998, 1295, 1460
HapII	CCGG	4 cut(s)	957, 999, 1325, 1681
HgaI	GACGC	2 cut(s)	311, 1173
HhaI	GCGC	4 cut(s)	129, 134, 338, 631
Hin1I	GRCGYC	2 cut(s)	303, 1184
Hin6I	GCGC	4 cut(s)	127, 132, 336, 629
HinP1I	GCGC	4 cut(s)	127, 132, 336, 629
HindIII	AAGCTT	2 cut(s)	285, 701
HinfI	GANTC	4 cut(s)	14, 370, 544, 808
HpaII	CCGG	4 cut(s)	957, 999, 1325, 1681
HphI	GGTGA	7 cut(s)	100, 464, 607, 872, 1235, 1297, 1388
Hpy166II	GTNNAC	2 cut(s)	656, 1249
Hpy188I	TCNGA	7 cut(s)	358, 381, 395, 576, 789, 884, 1191
Hpy188III	TCNNGA	5 cut(s)	43, 1073, 1112, 1259, 1435
Hpy8I	GTNNAC	2 cut(s)	656, 1249
Hpy99I	CGWCG	4 cut(s)	149, 221, 305, 410
HpyAV	CCTTC	6 cut(s)	80, 204, 328, 1343, 1636, 1694
HpyCH4III	ACNGT	3 cut(s)	390, 619, 1039
HpyCH4IV	ACGT	6 cut(s)	311, 340, 408, 435, 714, 818
HpyF10VI	GCNNNNNNNGC	6 cut(s)	329, 635, 709, 941, 1181, 1524
HpyF3I	CTNAG	5 cut(s)	357, 378, 537, 1212, 1598
HpySE526I	ACGT	6 cut(s)	311, 340, 408, 435, 714, 818
Hsp92I	GRCGYC	2 cut(s)	303, 1184
HspAI	GCGC	4 cut(s)	127, 132, 336, 629
Ksp22I	TGATCA	2 cut(s)	1378, 1626
Kzo9I	GATC	4 cut(s)	1378, 1425, 1626, 1672
LguI	GCTCTTC	1 cut(s)	1051
LmnI	GCTCC	3 cut(s)	63, 320, 401
Lsp1109I	GCAGC	5 cut(s)	116, 399, 402, 919, 1464
LweI	GCATC	2 cut(s)	57, 904
MaeI	CTAG	1 cut(s)	1146
MaeII	ACGT	6 cut(s)	311, 340, 408, 435, 714, 818
MaeIII	GTNAC	3 cut(s)	21, 613, 860
MalI	GATC	4 cut(s)	1380, 1427, 1628, 1674
MboI	GATC	4 cut(s)	1378, 1425, 1626, 1672
MboII	GAAGA	7 cut(s)	166, 602, 817, 1068, 1312, 1327, 1330
MfeI	CAATTG	1 cut(s)	647
MhlI	GDGCHC	4 cut(s)	317, 658, 911, 1681
MlsI	TGGCCA	1 cut(s)	5
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	1 cut(s)	23
MmeI	TCCRAC	2 cut(s)	268, 491
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	2 cut(s)	852, 1513
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	7 cut(s)	521, 987, 1023, 1371, 1440, 1590, 1695
MslI	CAYNNNNRTG	2 cut(s)	855, 1343
Msp20I	TGGCCA	1 cut(s)	5
MspI	CCGG	4 cut(s)	957, 999, 1325, 1681
MspR9I	CCNGG	3 cut(s)	1000, 1326, 1681
MteI	GCGCNGCGC	1 cut(s)	130
MunI	CAATTG	1 cut(s)	647
Mva1269I	GAATGC	2 cut(s)	520, 1513
MvnI	CGCG	3 cut(s)	164, 749, 1253
MwoI	GCNNNNNNNGC	6 cut(s)	329, 635, 709, 941, 1181, 1524
NciI	CCSGG	3 cut(s)	1000, 1326, 1681
NdeII	GATC	4 cut(s)	1378, 1425, 1626, 1672
NheI	GCTAGC	1 cut(s)	1145
NlaIV	GGNNCC	1 cut(s)	403
NmuCI	GTSAC	2 cut(s)	613, 860
NsbI	TGCGCA	1 cut(s)	337
NsiI	ATGCAT	2 cut(s)	852, 1513
NspI	RCATGY	1 cut(s)	683
NspV	TTCGAA	1 cut(s)	1640
PaeR7I	CTCGAG	1 cut(s)	122
PagI	TCATGA	1 cut(s)	1258
PceI	AGGCCT	1 cut(s)	228
PciSI	GCTCTTC	1 cut(s)	1051
PcsI	WCGNNNNNNNCGW	1 cut(s)	824
PctI	GAATGC	2 cut(s)	520, 1513
PfeI	GAWTC	3 cut(s)	370, 544, 808
PflFI	GACNNNGTC	1 cut(s)	277
PflMI	CCANNNNNTGG	2 cut(s)	207, 264
PkrI	GCNGC	8 cut(s)	131, 414, 417, 758, 934, 1255, 1459, 1479
PleI	GAGTC	1 cut(s)	22
PmaCI	CACGTG	2 cut(s)	312, 341
PmlI	CACGTG	2 cut(s)	312, 341
PpsI	GAGTC	1 cut(s)	22
Ppu21I	YACGTR	2 cut(s)	312, 341
PsiI	TTATAA	2 cut(s)	1091, 1650
PspCI	CACGTG	2 cut(s)	312, 341
PspFI	CCCAGC	2 cut(s)	1051, 1061
PspN4I	GGNNCC	1 cut(s)	403
PspXI	VCTCGAGB	1 cut(s)	122
PsyI	GACNNNGTC	1 cut(s)	277
RsaI	GTAC	5 cut(s)	269, 387, 746, 1122, 1337
RsaNI	GTAC	5 cut(s)	268, 386, 745, 1121, 1336
RseI	CAYNNNNRTG	2 cut(s)	855, 1343
SapI	GCTCTTC	1 cut(s)	1051
SaqAI	TTAA	7 cut(s)	521, 987, 1023, 1371, 1440, 1590, 1695
SatI	GCNGC	8 cut(s)	130, 413, 416, 757, 933, 1254, 1458, 1478
Sau3AI	GATC	4 cut(s)	1378, 1425, 1626, 1672
SchI	GAGTC	1 cut(s)	23
ScrFI	CCNGG	3 cut(s)	1000, 1326, 1681
SduI	GDGCHC	4 cut(s)	317, 658, 911, 1681
SfaNI	GCATC	2 cut(s)	57, 904
SfcI	CTRYAG	1 cut(s)	561
Sfr274I	CTCGAG	1 cut(s)	122
SfuI	TTCGAA	1 cut(s)	1640
SlaI	CTCGAG	1 cut(s)	122
SmiMI	CAYNNNNRTG	2 cut(s)	855, 1343
SmlI	CTYRAG	2 cut(s)	122, 872
SmoI	CTYRAG	2 cut(s)	122, 872
SseBI	AGGCCT	1 cut(s)	228
SsiI	CCGC	5 cut(s)	731, 756, 902, 1253, 1457
SspMI	CTAG	1 cut(s)	1146
StuI	AGGCCT	1 cut(s)	228
StyD4I	CCNGG	3 cut(s)	998, 1324, 1679
TaaI	ACNGT	3 cut(s)	390, 619, 1039
TaiI	ACGT	6 cut(s)	314, 343, 411, 438, 717, 821
TaqI	TCGA	7 cut(s)	123, 223, 300, 547, 992, 1640, 1675
TatI	WGTACW	1 cut(s)	385
TauI	GCSGC	3 cut(s)	759, 1256, 1460
TfiI	GAWTC	3 cut(s)	370, 544, 808
Tru1I	TTAA	7 cut(s)	521, 987, 1023, 1371, 1440, 1590, 1695
Tru9I	TTAA	7 cut(s)	521, 987, 1023, 1371, 1440, 1590, 1695
TscAI	CASTG	4 cut(s)	85, 559, 924, 1529
TseFI	GTSAC	2 cut(s)	613, 860
TseI	GCWGC	5 cut(s)	129, 412, 415, 932, 1477
Tsp45I	GTSAC	2 cut(s)	613, 860
TspDTI	ATGAA	4 cut(s)	498, 624, 809, 1313
TspRI	CASTG	4 cut(s)	85, 559, 924, 1529
Tth111I	GACNNNGTC	1 cut(s)	277
Van91I	CCANNNNNTGG	2 cut(s)	207, 264
VneI	GTGCAC	1 cut(s)	654
XapI	RAATTY	1 cut(s)	86
XceI	RCATGY	1 cut(s)	683
XhoI	CTCGAG	1 cut(s)	122
XspI	CTAG	1 cut(s)	1146
Zsp2I	ATGCAT	2 cut(s)	852, 1513

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.