Rroxscaffold_7G00195290

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0014380
Physical Location & Seq
Forward (+)
37539821 .. 37541548
1728 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00195290.1

Sequence Viewer

Length: 1701 bp
ATGCGACTCTCTCACCCGCATCAAACAGCTCCAAGCCCACCTCTTAACCATTGGCCGATTCCAACTCTACCCCTCCATCACCATCAAGCTCCTCGAGCTCTGTGCTCTTCCCCCATCGCCAATCTCCCTTACGCCACAACCCTCTTCCACCAACTCCAAAGCCCTTCCACCAGGCACTGGAACGCCGTCGTTCGAGGCCTCGCCCAGAGCCTCCAACCCACCCAAGCCATCACATGGTACGCCACCATGTCGCGCTCTTCCCAAAAGGTCGACGCGCTCACCTGCTCCTTCGCTCTCAAAGCGTGTGCACGCGCCTTGGCCTTGTCCGAGGCCATGCAGATTCACTCTCAGCTTCTGCGTTTCGGGTTTGGACGCGATGTGTTGTTGCGAACTACTTTGCTTGATGTGTATGCGAAAGTCGGGAATCTGGGTTTTGCACAGAAGGTGTTCGACGAAATGCCTGAGAGGGATGTAGCTTGTTGGAATGCTTTGGTTGCTGGGTTGGCTCAGGGGAGTCAACCCAGTGAGGCTTTAGCTTTGTTTGAGAGAATGACTGAGGAGGAGGGGTTAAAGCCCAATGAAGTTACTGTGCTCGGTGCGCTCTCGGCGTGTTCTCAATTGGGTGCATTGAGAGGTGGAGAGAGAATACATACCTATATAGTGAATGCCAAGCTTGATACGCATGTCATTGTTTGCAATGCGGTTATTGATATGTATGCGAAATGCGGGTTCGTGGATAGAGCATATGAGGTGTTTGAGAATATGAGGTGTGGGAGGAATTTGATAACTTGGAATACGATGATAATGGCATTTGCTATGCATGGCGATGGTTGTAAAGCGCTTGAGCTTTTTGAGAAGATGGGTAAAAATGGAGTGTGCCCTGATGCAGTGTCGTATCTGGCTGCGTTGTGTGCGTGTAATCATGCAGGACTTGTGGAAGATGGGGTTAAGTTATTTAATTCAATGGCGGGGAGTGGGATGGTACCGAATGTTAAGCATTATGGGACTGTGGTTGATTTGTTGGGACGAGCTGGGCGACTTCAAGAGGCTTATGAGATTGTGAAATCTATGCCGATGTTTCCTGATGTAGTACTGTGGCAGACTTTGCTTGGTGCCAGCAAGACCTATGGGAATGTGGAAATGGCAGAGATGGCGTCAAGGAACTTGGTTGAGTTGGGGTCTGAGGGTTGTGGTGATTTCGTGTTGTTATCAAATGTTTATGCGGCTCATAATAGATGGGATGATGTAGGCAGGGTGCGGGAAGCCATGAAGAGAAGGGATGTGAAGAAGATACCTGGTTTCGGTTACATAGAAGTTGAAGGTGTGATACACAAATTTGTGAATGGTGATCAAAACCATGTTAATAGGCATGATATTTATTCAAAGCTAGATGAGATCAAATGCAGGATTAAAGCCTATGGATATGTTGCAAAGACAAATCATGTGTTGCATGATATTGGGGAGGAGGACAAAGAAAATGCATTGAGCTACCATTGTGAGAAGTTGGCTGTGGCTTTTGGTTTGATCAGCACTGATGAGGGGACACCAATTCAGGTGATTAAGAACCTAAGAATATGTGATGATTGTCATGTCGTGATGAAACTTATTTCAGAAATTTATAGTAGGGAAATTATTGTAAGGGATCGGGCCCGGTTTCACAGATTTCAGGATGGACTTTGTTCTTGCAGAGATTATTGGTGA

Protein Analysis

566

Amino Acids

63.0

Weight (kDa)

7.07

Isoelectric Point (pI)

40.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 156 - 204 4.5e-06 PPR repeat family
PPR_2 PF13041 260 - 306 6.9e-10 PPR repeat family
PPR PF01535 262 - 292 2.5e-06 PPR repeat
E_motif PF20431 376 - 438 7.5e-19 E motif
DYW_deaminase PF14432 474 - 566 1.6e-38 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014380)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34160 AT1G34160
fragaria_vesca FvH4_2g12730
malus_domestica MD05G1037500.v1.1 MD10G1041300.v1.1
prunus_persica Prupe.8G045500_v2.0.a1 Prupe.8G045500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0273501
rosa_laevigata RLG00000013612
rosa_multiflora Rmu_co8087438.1_g000001 Rmu_sc0026438.1_g000001
rosa_roxburghii Rroxscaffold_7G00195290
rosa_rugosa Rorug06G0075900
rosa_samantha Rh6AG190600 Rh6BG194100 Rh6DG185200
rosa_wichuraiana Rw6G016680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 290
Acc36I ACCTGC 1 cut(s) 290
Acc65I GGTACC 1 cut(s) 982
AccB1I GGYRCC 2 cut(s) 982, 1112
AccB7I CCANNNNNTGG 2 cut(s) 177, 234
AccI GTMKAC 1 cut(s) 270
AccII CGCG 4 cut(s) 253, 275, 312, 375
AciI CCGC 6 cut(s) 17, 701, 726, 968, 1223, 1258
AclWI GGATC 1 cut(s) 1650
AcoI YGGCCR 1 cut(s) 53
AcsI RAATTY 3 cut(s) 778, 1334, 1614
AcyI GRCGYC 1 cut(s) 1154
AfaI GTAC 3 cut(s) 239, 984, 1092
AfeI AGCGCT 1 cut(s) 840
AfiI CCNNNNNNNGG 3 cut(s) 177, 234, 1301
AgsI TTSAA 4 cut(s) 963, 1043, 1319, 1383
AjnI CCWGG 2 cut(s) 170, 1294
Alw21I GWGCWC 4 cut(s) 100, 107, 310, 594
Alw44I GTGCAC 1 cut(s) 306
AlwI GGATC 1 cut(s) 1650
AlwNI CAGNNNCTG 2 cut(s) 177, 355
Ama87I CYCGRG 1 cut(s) 93
Aor51HI AGCGCT 1 cut(s) 840
AoxI GGCC 5 cut(s) 53, 196, 318, 330, 1647
ApaI GGGCCC 1 cut(s) 1651
ApaLI GTGCAC 1 cut(s) 306
ApeKI GCWGC 1 cut(s) 902
ApoI RAATTY 3 cut(s) 778, 1334, 1614
Asp700I GAANNNNTTC 1 cut(s) 446
Asp718I GGTACC 1 cut(s) 982
AspLEI GCGC 5 cut(s) 255, 277, 314, 601, 841
AspS9I GGNCC 2 cut(s) 1647, 1648
AsuC2I CCSGG 1 cut(s) 1651
AsuHPI GGTGA 6 cut(s) 5, 71, 271, 1205, 1358, 1567
AvaI CYCGRG 1 cut(s) 93
BaeGI GKGCMC 3 cut(s) 310, 881, 1651
BaeI ACNNNNGTAYC 2 cut(s) 669, 702
BanI GGYRCC 2 cut(s) 982, 1112
BanII GRGCYC 2 cut(s) 100, 1651
BarI GAAGNNNNNNTAC 2 cut(s) 1311, 1343
Bbv12I GWGCWC 4 cut(s) 100, 107, 310, 594
BbvI GCAGC 1 cut(s) 889
BceAI ACGGC 1 cut(s) 170
BcgI CGANNNNNNTGC 2 cut(s) 84, 118
BciT130I CCWGG 2 cut(s) 172, 1296
BclI TGATCA 2 cut(s) 1348, 1524
BcnI CCSGG 1 cut(s) 1651
BfaI CTAG 1 cut(s) 1388
BfoI RGCGCY 1 cut(s) 842
BfuAI ACCTGC 1 cut(s) 290
BisI GCNGC 2 cut(s) 903, 1224
BlsI GCNGC 2 cut(s) 904, 1225
BmcAI AGTACT 1 cut(s) 1092
Bme1390I CCNGG 3 cut(s) 172, 1296, 1651
BmeT110I CYCGRG 1 cut(s) 93
BmgT120I GGNCC 2 cut(s) 1647, 1648
BmiI GGNNCC 3 cut(s) 984, 1114, 1649
BmrFI CCNGG 3 cut(s) 172, 1296, 1651
BmrI ACTGGG 1 cut(s) 516
BmsI GCATC 2 cut(s) 28, 874
BmuI ACTGGG 1 cut(s) 516
Bpu10I CCTNAGC 1 cut(s) 507
BpuEI CTTGAG 1 cut(s) 863
BpuMI CCSGG 1 cut(s) 1651
BsaHI GRCGYC 1 cut(s) 1154
BsaJI CCNNGG 2 cut(s) 315, 327
BsaXI ACNNNNNCTCC 2 cut(s) 551, 581
Bsc4I CCNNNNNNNGG 3 cut(s) 177, 234, 1301
Bse1I ACTGG 2 cut(s) 182, 522
Bse3DI GCAATG 1 cut(s) 703
BseBI CCWGG 2 cut(s) 172, 1296
BseDI CCNNGG 2 cut(s) 315, 327
BseGI GGATG 5 cut(s) 475, 984, 1246, 1285, 1675
BseLI CCNNNNNNNGG 3 cut(s) 177, 234, 1301
BseMI GCAATG 1 cut(s) 703
BseMII CTCAG 5 cut(s) 362, 453, 521, 546, 1173
BseNI ACTGG 2 cut(s) 182, 522
BseRI GAGGAG 4 cut(s) 81, 572, 575, 1478
BseSI GKGCMC 3 cut(s) 310, 881, 1651
BseXI GCAGC 1 cut(s) 889
BseYI CCCAGC 2 cut(s) 497, 1031
Bsh1236I CGCG 4 cut(s) 253, 275, 312, 375
BshFI GGCC 5 cut(s) 55, 198, 320, 332, 1649
BshNI GGYRCC 2 cut(s) 982, 1112
BsiHKAI GWGCWC 4 cut(s) 100, 107, 310, 594
BsiHKCI CYCGRG 1 cut(s) 93
BsiSI CCGG 1 cut(s) 1651
BslFI GGGAC 3 cut(s) 1018, 1038, 1555
BslI CCNNNNNNNGG 3 cut(s) 177, 234, 1301
BsmFI GGGAC 3 cut(s) 1018, 1038, 1555
BsmI GAATGC 2 cut(s) 490, 670
BsnI GGCC 5 cut(s) 55, 198, 320, 332, 1649
BsoBI CYCGRG 1 cut(s) 93
Bsp120I GGGCCC 1 cut(s) 1647
Bsp1286I GDGCHC 6 cut(s) 100, 107, 310, 594, 881, 1651
Bsp143I GATC 4 cut(s) 1348, 1395, 1524, 1642
BspACI CCGC 6 cut(s) 17, 701, 726, 968, 1223, 1258
BspANI GGCC 5 cut(s) 55, 198, 320, 332, 1649
BspCNI CTCAG 5 cut(s) 361, 454, 520, 547, 1174
BspFNI CGCG 4 cut(s) 253, 275, 312, 375
BspLI GGNNCC 3 cut(s) 984, 1114, 1649
BspMI ACCTGC 1 cut(s) 290
BspPI GGATC 1 cut(s) 1650
BspQI GCTCTTC 2 cut(s) 112, 262
BspT107I GGYRCC 2 cut(s) 982, 1112
BsrDI GCAATG 1 cut(s) 703
BsrI ACTGG 2 cut(s) 182, 522
BssECI CCNNGG 2 cut(s) 315, 327
BssMI GATC 4 cut(s) 1348, 1395, 1524, 1642
BssNI GRCGYC 1 cut(s) 1154
BssT1I CCWWGG 1 cut(s) 315
Bst2UI CCWGG 2 cut(s) 172, 1296
Bst4CI ACNGT 3 cut(s) 589, 1009, 1095
Bst6I CTCTTC 4 cut(s) 112, 149, 262, 1265
BstACI GRCGYC 1 cut(s) 1154
BstAPI GCANNNNNTGC 1 cut(s) 1105
BstC8I GCNNGC 2 cut(s) 310, 1117
BstDEI CTNAG 6 cut(s) 348, 462, 507, 555, 1182, 1568
BstF5I GGATG 5 cut(s) 475, 984, 1246, 1285, 1675
BstFNI CGCG 4 cut(s) 253, 275, 312, 375
BstH2I RGCGCY 1 cut(s) 842
BstHHI GCGC 5 cut(s) 255, 277, 314, 601, 841
BstKTI GATC 4 cut(s) 1351, 1398, 1527, 1645
BstMBI GATC 4 cut(s) 1348, 1395, 1524, 1642
BstNI CCWGG 2 cut(s) 172, 1296
BstNSI RCATGY 1 cut(s) 686
BstSCI CCNGG 3 cut(s) 170, 1294, 1649
BstSLI GKGCMC 3 cut(s) 310, 881, 1651
BstUI CGCG 4 cut(s) 253, 275, 312, 375
BstV1I GCAGC 1 cut(s) 889
BsuRI GGCC 5 cut(s) 55, 198, 320, 332, 1649
BtgZI GCGATG 3 cut(s) 100, 390, 840
BtsCI GGATG 5 cut(s) 475, 984, 1246, 1285, 1675
BtsI GCAGTG 1 cut(s) 894
BtsIMutI CAGTG 4 cut(s) 175, 529, 894, 1530
BveI ACCTGC 1 cut(s) 290
Cac8I GCNNGC 2 cut(s) 310, 1117
CaiI CAGNNNCTG 2 cut(s) 177, 355
CfoI GCGC 5 cut(s) 255, 277, 314, 601, 841
Cfr13I GGNCC 2 cut(s) 1647, 1648
CseI GACGC 3 cut(s) 281, 381, 1143
CsiI ACCWGGT 1 cut(s) 1294
Csp6I GTAC 3 cut(s) 238, 983, 1091
CviQI GTAC 3 cut(s) 238, 983, 1091
DdeI CTNAG 6 cut(s) 348, 462, 507, 555, 1182, 1568
DpnI GATC 4 cut(s) 1350, 1397, 1526, 1644
DpnII GATC 4 cut(s) 1348, 1395, 1524, 1642
EaeI YGGCCR 1 cut(s) 53
Eam1104I CTCTTC 4 cut(s) 112, 149, 262, 1265
EarI CTCTTC 4 cut(s) 112, 149, 262, 1265
Ecl136II GAGCTC 1 cut(s) 98
Eco130I CCWWGG 1 cut(s) 315
Eco147I AGGCCT 1 cut(s) 198
Eco24I GRGCYC 2 cut(s) 100, 1651
Eco47III AGCGCT 1 cut(s) 840
Eco53kI GAGCTC 1 cut(s) 98
Eco88I CYCGRG 1 cut(s) 93
EcoICRI GAGCTC 1 cut(s) 98
EcoRII CCWGG 2 cut(s) 170, 1294
EcoT14I CCWWGG 1 cut(s) 315
EcoT22I ATGCAT 2 cut(s) 822, 1483
EcoT38I GRGCYC 2 cut(s) 100, 1651
ErhI CCWWGG 1 cut(s) 315
FaqI GGGAC 3 cut(s) 1018, 1038, 1555
FauI CCCGC 4 cut(s) 24, 719, 961, 1251
FauNDI CATATG 1 cut(s) 745
FbaI TGATCA 2 cut(s) 1348, 1524
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 2 cut(s) 903, 1224
FokI GGATG 5 cut(s) 482, 991, 1253, 1292, 1682
FriOI GRGCYC 2 cut(s) 100, 1651
Fsp4HI GCNGC 2 cut(s) 903, 1224
FspBI CTAG 1 cut(s) 1388
GlaI GCGC 5 cut(s) 254, 276, 313, 600, 840
GluI GCNGC 2 cut(s) 903, 1224
GsaI CCCAGC 2 cut(s) 501, 1035
HaeII RGCGCY 1 cut(s) 842
HaeIII GGCC 5 cut(s) 55, 198, 320, 332, 1649
HapII CCGG 1 cut(s) 1651
HgaI GACGC 3 cut(s) 281, 381, 1143
HhaI GCGC 5 cut(s) 255, 277, 314, 601, 841
Hin1I GRCGYC 1 cut(s) 1154
Hin6I GCGC 5 cut(s) 253, 275, 312, 599, 839
HinP1I GCGC 5 cut(s) 253, 275, 312, 599, 839
HincII GTYRAC 2 cut(s) 271, 518
HindII GTYRAC 2 cut(s) 271, 518
HindIII AAGCTT 1 cut(s) 671
HinfI GANTC 5 cut(s) 6, 58, 340, 424, 514
HpaII CCGG 1 cut(s) 1651
HphI GGTGA 6 cut(s) 5, 71, 271, 1205, 1358, 1567
Hpy166II GTNNAC 3 cut(s) 271, 308, 518
Hpy188I TCNGA 3 cut(s) 328, 1183, 1612
Hpy188III TCNNGA 5 cut(s) 421, 1043, 1082, 1594, 1667
Hpy8I GTNNAC 3 cut(s) 271, 308, 518
Hpy99I CGWCG 3 cut(s) 191, 275, 455
HpyAV CCTTC 5 cut(s) 174, 298, 436, 1269, 1313
HpyCH4III ACNGT 3 cut(s) 589, 1009, 1095
HpyF3I CTNAG 6 cut(s) 348, 462, 507, 555, 1182, 1568
Hsp92I GRCGYC 1 cut(s) 1154
HspAI GCGC 5 cut(s) 253, 275, 312, 599, 839
KpnI GGTACC 1 cut(s) 986
Ksp22I TGATCA 2 cut(s) 1348, 1524
Kzo9I GATC 4 cut(s) 1348, 1395, 1524, 1642
LguI GCTCTTC 2 cut(s) 112, 262
LmnI GCTCC 3 cut(s) 34, 94, 290
Lsp1109I GCAGC 1 cut(s) 889
LweI GCATC 2 cut(s) 28, 874
MabI ACCWGGT 1 cut(s) 1294
MaeI CTAG 1 cut(s) 1388
MaeIII GTNAC 2 cut(s) 583, 1304
MalI GATC 4 cut(s) 1350, 1397, 1526, 1644
MboI GATC 4 cut(s) 1348, 1395, 1524, 1642
MboII GAAGA 8 cut(s) 99, 136, 249, 868, 950, 1282, 1297, 1300
MfeI CAATTG 1 cut(s) 617
MhlI GDGCHC 6 cut(s) 100, 107, 310, 594, 881, 1651
MluCI AATT 7 cut(s) 617, 778, 958, 1334, 1548, 1614, 1629
MlyI GAGTC 1 cut(s) 523
MmeI TCCRAC 3 cut(s) 86, 238, 461
Mph1103I ATGCAT 2 cut(s) 822, 1483
MroXI GAANNNNTTC 1 cut(s) 446
MseI TTAA 8 cut(s) 45, 569, 948, 957, 993, 1362, 1410, 1560
MslI CAYNNNNRTG 1 cut(s) 825
MspI CCGG 1 cut(s) 1651
MspR9I CCNGG 3 cut(s) 172, 1296, 1651
MunI CAATTG 1 cut(s) 617
Mva1269I GAATGC 2 cut(s) 490, 670
MvaI CCWGG 2 cut(s) 172, 1296
MvnI CGCG 4 cut(s) 253, 275, 312, 375
NciI CCSGG 1 cut(s) 1651
NdeI CATATG 1 cut(s) 745
NdeII GATC 4 cut(s) 1348, 1395, 1524, 1642
NlaIV GGNNCC 3 cut(s) 984, 1114, 1649
NmeAIII GCCGAG 1 cut(s) 584
NsiI ATGCAT 2 cut(s) 822, 1483
NspI RCATGY 1 cut(s) 686
PaeR7I CTCGAG 1 cut(s) 93
PaqCI CACCTGC 1 cut(s) 290
PceI AGGCCT 1 cut(s) 198
PciSI GCTCTTC 2 cut(s) 112, 262
PcsI WCGNNNNNNNCGW 1 cut(s) 1033
PctI GAATGC 2 cut(s) 490, 670
PdmI GAANNNNTTC 1 cut(s) 446
PfeI GAWTC 3 cut(s) 58, 340, 424
PflMI CCANNNNNTGG 2 cut(s) 177, 234
PkrI GCNGC 2 cut(s) 904, 1225
PleI GAGTC 1 cut(s) 522
PpsI GAGTC 1 cut(s) 522
Psp124BI GAGCTC 1 cut(s) 100
Psp6I CCWGG 2 cut(s) 170, 1294
PspFI CCCAGC 2 cut(s) 497, 1031
PspGI CCWGG 2 cut(s) 170, 1294
PspN4I GGNNCC 3 cut(s) 984, 1114, 1649
PspOMI GGGCCC 1 cut(s) 1647
PspPI GGNCC 2 cut(s) 1647, 1648
PspXI VCTCGAGB 1 cut(s) 93
PstNI CAGNNNCTG 2 cut(s) 177, 355
RsaI GTAC 3 cut(s) 239, 984, 1092
RsaNI GTAC 3 cut(s) 238, 983, 1091
RseI CAYNNNNRTG 1 cut(s) 825
SacI GAGCTC 1 cut(s) 100
SalI GTCGAC 1 cut(s) 269
SapI GCTCTTC 2 cut(s) 112, 262
SaqAI TTAA 8 cut(s) 45, 569, 948, 957, 993, 1362, 1410, 1560
SatI GCNGC 2 cut(s) 903, 1224
Sau3AI GATC 4 cut(s) 1348, 1395, 1524, 1642
Sau96I GGNCC 2 cut(s) 1647, 1648
ScaI AGTACT 1 cut(s) 1092
SchI GAGTC 1 cut(s) 523
ScrFI CCNGG 3 cut(s) 172, 1296, 1651
SduI GDGCHC 6 cut(s) 100, 107, 310, 594, 881, 1651
SexAI ACCWGGT 1 cut(s) 1294
SfaNI GCATC 2 cut(s) 28, 874
Sfr274I CTCGAG 1 cut(s) 93
SlaI CTCGAG 1 cut(s) 93
SmiMI CAYNNNNRTG 1 cut(s) 825
SmlI CTYRAG 2 cut(s) 93, 842
SmoI CTYRAG 2 cut(s) 93, 842
Sse9I AATT 7 cut(s) 617, 778, 958, 1334, 1548, 1614, 1629
SseBI AGGCCT 1 cut(s) 198
SsiI CCGC 6 cut(s) 17, 701, 726, 968, 1223, 1258
SspMI CTAG 1 cut(s) 1388
SstI GAGCTC 1 cut(s) 100
StuI AGGCCT 1 cut(s) 198
StyD4I CCNGG 3 cut(s) 170, 1294, 1649
StyI CCWWGG 1 cut(s) 315
TaaI ACNGT 3 cut(s) 589, 1009, 1095
TaqI TCGA 4 cut(s) 94, 193, 270, 450
TasI AATT 7 cut(s) 617, 778, 958, 1334, 1548, 1614, 1629
TatI WGTACW 1 cut(s) 1090
TauI GCSGC 1 cut(s) 1226
TfiI GAWTC 3 cut(s) 58, 340, 424
Tru1I TTAA 8 cut(s) 45, 569, 948, 957, 993, 1362, 1410, 1560
Tru9I TTAA 8 cut(s) 45, 569, 948, 957, 993, 1362, 1410, 1560
TscAI CASTG 4 cut(s) 182, 529, 894, 1537
TseI GCWGC 1 cut(s) 902
TspDTI ATGAA 3 cut(s) 594, 1283, 1613
TspRI CASTG 4 cut(s) 182, 529, 894, 1537
Van91I CCANNNNNTGG 2 cut(s) 177, 234
VneI GTGCAC 1 cut(s) 306
XapI RAATTY 3 cut(s) 778, 1334, 1614
XceI RCATGY 1 cut(s) 686
XhoI CTCGAG 1 cut(s) 93
XmiI GTMKAC 1 cut(s) 270
XmnI GAANNNNTTC 1 cut(s) 446
XspI CTAG 1 cut(s) 1388
ZrmI AGTACT 1 cut(s) 1092
Zsp2I ATGCAT 2 cut(s) 822, 1483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.