MD10G1042100.v1.1

AarF domain-containing protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009372
Physical Location & Seq
Reverse (-)
5427366 .. 5438022
10657 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1042100.v1.1.491

Sequence Viewer

Length: 2127 bp
ATGATTCTCCGCCTTCCCTCGCCTTCTCTCCCCTCCTCCTCCCTTTCCCCTTCCGCACCCAACCCCCTCCGGCGGTTCTCTTCATCCTCTTTCCCCCGCCCCCGCCGTCGTCGCTCCATTCTGCGATGCGCCGCCGGAAACGACGTCGTCGAACTCGACGCCTTCACCAAGAAGTCGGGCTACATCTTCGAGCTCAGCGCCCCCGAGGCCAACTCCATAGCCGACTACGATATCTCCAAAATCGGCGCCATTTACCGGCGGAAGCCTCTGATTCTTCTCCGCCGACTCTCTCAGATTGCCTTCACTTTCGGCAAGTGGTTCGCGGTTCGCTACATTGATAACCTGACGGAGCGCTCTGACCGAATGTTCGAGGTTAGAGCTGCCGAGCTTCGGAATATTCTGGTAGAACTCGGACCGGCATATATCAAAATTGCTCAGGCCATCTCATCTCGACCGGACTTGATACCACCGTCATACTTGGATGAGCTCTCATTGTTGCAAGATCGAATATCTCCATTTTTTACGGAAGTTGCTATAAACACAATAGAACAAGAACTCGGTTTACCGATAGAAGAGCTTTTCTCAGAGATCTCACCGGAACCTGTAGCTGCAGCTTCCCTTGGACAGGTTTATCAAGCCAGGCTTCGCCGTACGGGACAGCTTGTTGCAGTAAAAGTGCAAAGGCCAGGAGTACAAGCTGCTATTTCCTTAGACATATTGATCTTGCGTTTTCTAGCAGGTCTACTTAGGAGAATAAGAAAATTAAATACTGATCTTCAGGCAGTTGTTGATGAATGGGCATCAAGTCTTTTTCGGGAGATGGACTACAGAACTGAAGCAAATAATGGTATCAGATTCAGACAGCTATACGGTGGTATACCCGATGTTTTGGTTCCGGAAATGTATCTGGATTACACAACTCGCAGGGTTCTTGTGATGGAATGGGTGGAGGGGCAGAAGCTGTCAGAAGTGAATGATCTTTACTTGGTTGAGGTCGGGACGTACTGCTCATTCAATCAACTTCTGGAATGTGGATTCTACCATGCAGATCCACACCCTGGAAATCTTCTTCGTACATTTGATGGGAAACTTGCTTATTTAGATTTTGGTATGATGGGTGAATTTAATCAAAAACTCCGCATTGGATTTATTGAAGCTTCTCTCCATCTTGTAAACCGTGATTTTGGTGCATTGGCTAAGGACTTTGTTACTCTTGGATTTATTCCACCAACTGCAGACAAGGAAGCTGTTACAAAGGCTTTAACAGGTGTCTTTCAGAATGCTGTTGACAAAGGGGTGCGCAATATTAGCTTTGGAGACCTATTAGGAGATTTAGGAGCCACCATGTACAAGTTCAAATTTCGTATACCTTCTTATTTTTCTCTTGTCATTCGAAGCCTTGCCGTATTGGAAGGTGTTGCCATTGGGTTCAACCCAGATTACAAAGTTTTAGGTAGCACATACCCATGGATTGCTCGAAAAGTATTAACAGACAGCTCAATAGAGCTCAAGTCCTCCCTGCAGGCCCTTCTTTATGAGGATGGCGTTTTCAATATCGACCGTCTAGAGTCTTTACTTTCTGAGGCTCTCCGTGCTAGAACAGAGAAGGTCGTGTCGAGAAAGCAAGAAGATGATGCTAATTCTAGGGTAGTTGTCAAACAAGTCCTTTCCTTTATTTTGGCAGAGAAGGGTGCATTTGTGAGGGATCTACTTCTTCAAGAATTTGTTAAGGGATTGGATGCACTTGGGCTGGCAACTTTGGACTCTGTTAGGACAAGTATACCTTTTGCTGCTACCTTTTCCATCTCAACAATGACCAATGAAGACAAAATCAACTTAAGAACACTCCGTCGCCTTCTGCGATTGCTGTCAGGGTTGCAAAGAAATGAAAACTCCAAACCAGTAATTAGAGACGATACCCCATACAAGAATCAGCAAATGAACTCGGATGAAGCAGCATTAGTTTTCAACCAACTTGCATCCGTTCAAGAAATTCTACCTCTCCTTTCTGTTCTTCCTGAGCTTCCACCAGAATCACAACAGCAGTTGCTTAATCTCCCAGCTGATCTTACAGGACGATTAATTTCCCGTGCTGCTGCAAGGACAATCCGAAGGATTGTTTTATGA

Protein Analysis

709

Amino Acids

79.5

Weight (kDa)

8.9

Isoelectric Point (pI)

50.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 166 - 400 8e-68 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 147
Acc16I TGCGCA 1 cut(s) 1301
Acc36I ACCTGC 1 cut(s) 728
AccB1I GGYRCC 1 cut(s) 245
AccB7I CCANNNNNTGG 1 cut(s) 1058
AccI GTMKAC 4 cut(s) 742, 877, 1366, 1780
AccII CGCG 1 cut(s) 323
AccIII TCCGGA 1 cut(s) 895
AclWI GGATC 2 cut(s) 1043, 1713
AcsI RAATTY 4 cut(s) 1121, 1358, 1721, 1992
AcuI CTGAAG 2 cut(s) 761, 855
AcyI GRCGYC 3 cut(s) 144, 159, 246
AfaI GTAC 5 cut(s) 652, 693, 1004, 1075, 1349
AfeI AGCGCT 1 cut(s) 353
AfiI CCNNNNNNNGG 5 cut(s) 72, 255, 390, 625, 1058
AflII CTTAAG 1 cut(s) 1837
AgsI TTSAA 8 cut(s) 1015, 1154, 1357, 1432, 1552, 1718, 1969, 1988
AjnI CCWGG 3 cut(s) 638, 685, 1057
Alw21I GWGCWC 3 cut(s) 195, 489, 1509
Alw26I GTCTC 2 cut(s) 1311, 1905
AlwI GGATC 2 cut(s) 1043, 1713
AlwNI CAGNNNCTG 1 cut(s) 961
Ama87I CYCGRG 1 cut(s) 203
Aor13HI TCCGGA 1 cut(s) 895
Aor51HI AGCGCT 1 cut(s) 353
AoxI GGCC 4 cut(s) 207, 438, 683, 1524
ApeKI GCWGC 8 cut(s) 380, 608, 611, 698, 1790, 1955, 2093, 2096
ApoI RAATTY 4 cut(s) 1121, 1358, 1721, 1992
ArsI GACNNNNNNTTYG 2 cut(s) 350, 382
AseI ATTAAT 1 cut(s) 2081
AspLEI GCGC 5 cut(s) 131, 200, 248, 354, 1302
AspS9I GGNCC 2 cut(s) 413, 1525
AsuHPI GGTGA 3 cut(s) 157, 585, 1130
AsuII TTCGAA 1 cut(s) 1393
AvaI CYCGRG 1 cut(s) 203
AvaII GGWCC 1 cut(s) 413
BaeI ACNNNNGTAYC 2 cut(s) 455, 488
BanI GGYRCC 1 cut(s) 245
BanII GRGCYC 3 cut(s) 195, 489, 1509
BarI GAAGNNNNNNTAC 2 cut(s) 164, 196
BbsI GAAGAC 1 cut(s) 1830
Bbv12I GWGCWC 3 cut(s) 195, 489, 1509
BbvI GCAGC 8 cut(s) 367, 595, 623, 685, 1777, 1967, 2080, 2083
BccI CCATC 8 cut(s) 449, 814, 931, 1076, 1108, 1173, 1535, 1811
BceAI ACGGC 3 cut(s) 90, 633, 1388
BcgI CGANNNNNNTGC 2 cut(s) 301, 335
BciT130I CCWGG 3 cut(s) 640, 687, 1059
BcoDI GTCTC 2 cut(s) 1311, 1905
BfaI CTAG 4 cut(s) 734, 1565, 1596, 1644
BfmI CTRYAG 5 cut(s) 603, 609, 826, 1233, 1520
BfoI RGCGCY 3 cut(s) 201, 249, 355
BfrI CTTAAG 1 cut(s) 1837
BfuAI ACCTGC 1 cut(s) 728
BglI GCCNNNNNGGC 1 cut(s) 206
BglII AGATCT 1 cut(s) 588
BisI GCNGC 9 cut(s) 132, 381, 609, 612, 699, 1791, 1956, 2094, 2097
BlpI GCTNAGC 1 cut(s) 194
BlsI GCNGC 9 cut(s) 133, 382, 610, 613, 700, 1792, 1957, 2095, 2098
Bme1390I CCNGG 3 cut(s) 640, 687, 1059
Bme18I GGWCC 1 cut(s) 413
BmeT110I CYCGRG 1 cut(s) 203
BmgT120I GGNCC 2 cut(s) 413, 1525
BmiI GGNNCC 4 cut(s) 247, 600, 894, 1339
BmrFI CCNGG 3 cut(s) 640, 687, 1059
BmsI GCATC 5 cut(s) 116, 809, 1624, 1729, 1988
BpiI GAAGAC 1 cut(s) 1830
BplI GAGNNNNNCTC 4 cut(s) 197, 229, 566, 598
Bpu10I CCTNAGC 3 cut(s) 435, 1197, 2019
Bpu1102I GCTNAGC 1 cut(s) 194
Bpu14I TTCGAA 1 cut(s) 1393
BpuEI CTTGAG 1 cut(s) 1493
BsaHI GRCGYC 3 cut(s) 144, 159, 246
BsaI GGTCTC 1 cut(s) 1311
BsaJI CCNNGG 4 cut(s) 204, 619, 1057, 1466
BsaWI WCCGGW 3 cut(s) 454, 595, 895
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 5 cut(s) 72, 255, 390, 625, 1058
Bse118I RCCGGY 2 cut(s) 255, 415
Bse1I ACTGG 1 cut(s) 1901
BseAI TCCGGA 1 cut(s) 895
BseBI CCWGG 3 cut(s) 640, 687, 1059
BseDI CCNNGG 4 cut(s) 204, 619, 1057, 1466
BseGI GGATG 6 cut(s) 83, 487, 1546, 1744, 1954, 1979
BseLI CCNNNNNNNGG 5 cut(s) 72, 255, 390, 625, 1058
BseMII CTCAG 6 cut(s) 208, 305, 449, 597, 1572, 2010
BseNI ACTGG 1 cut(s) 1901
BseRI GAGGAG 2 cut(s) 25, 28
BseXI GCAGC 8 cut(s) 367, 595, 623, 685, 1777, 1967, 2080, 2083
BseYI CCCAGC 1 cut(s) 2059
Bsh1236I CGCG 1 cut(s) 323
Bsh1285I CGRYCG 2 cut(s) 455, 1561
BshFI GGCC 4 cut(s) 209, 440, 685, 1526
BshNI GGYRCC 1 cut(s) 245
BsiEI CGRYCG 2 cut(s) 455, 1561
BsiHKAI GWGCWC 3 cut(s) 195, 489, 1509
BsiHKCI CYCGRG 1 cut(s) 203
BsiSI CCGG 7 cut(s) 70, 135, 256, 416, 455, 596, 896
BsiWI CGTACG 1 cut(s) 650
BslFI GGGAC 2 cut(s) 669, 1012
BslI CCNNNNNNNGG 5 cut(s) 72, 255, 390, 625, 1058
BsmAI GTCTC 2 cut(s) 1311, 1905
BsmBI CGTCTC 1 cut(s) 1905
BsmFI GGGAC 2 cut(s) 669, 1012
BsmI GAATGC 1 cut(s) 1285
BsnI GGCC 4 cut(s) 209, 440, 685, 1526
Bso31I GGTCTC 1 cut(s) 1311
BsoBI CYCGRG 1 cut(s) 203
Bsp119I TTCGAA 1 cut(s) 1393
Bsp1286I GDGCHC 3 cut(s) 195, 489, 1509
Bsp13I TCCGGA 1 cut(s) 895
Bsp1407I TGTACA 1 cut(s) 1347
Bsp143I GATC 8 cut(s) 502, 588, 720, 772, 976, 1048, 1705, 2065
Bsp1720I GCTNAGC 1 cut(s) 194
Bsp19I CCATGG 1 cut(s) 1466
BspANI GGCC 4 cut(s) 209, 440, 685, 1526
BspCNI CTCAG 6 cut(s) 207, 304, 448, 596, 1573, 2011
BspEI TCCGGA 1 cut(s) 895
BspFNI CGCG 1 cut(s) 323
BspLI GGNNCC 4 cut(s) 247, 600, 894, 1339
BspMAI CTGCAG 3 cut(s) 613, 1237, 1524
BspMI ACCTGC 1 cut(s) 728
BspPI GGATC 2 cut(s) 1043, 1713
BspQI GCTCTTC 1 cut(s) 567
BspT104I TTCGAA 1 cut(s) 1393
BspT107I GGYRCC 1 cut(s) 245
BspTI CTTAAG 1 cut(s) 1837
BspTNI GGTCTC 1 cut(s) 1311
BsrFI RCCGGY 2 cut(s) 255, 415
BsrGI TGTACA 1 cut(s) 1347
BsrI ACTGG 1 cut(s) 1901
BssAI RCCGGY 2 cut(s) 255, 415
BssECI CCNNGG 4 cut(s) 204, 619, 1057, 1466
BssMI GATC 8 cut(s) 502, 588, 720, 772, 976, 1048, 1705, 2065
BssNAI GTATAC 3 cut(s) 878, 1367, 1781
BssNI GRCGYC 3 cut(s) 144, 159, 246
BssT1I CCWWGG 2 cut(s) 619, 1466
Bst1107I GTATAC 3 cut(s) 878, 1367, 1781
Bst2UI CCWGG 3 cut(s) 640, 687, 1059
Bst4CI ACNGT 4 cut(s) 471, 872, 1178, 1562
Bst6I CTCTTC 2 cut(s) 85, 567
BstACI GRCGYC 3 cut(s) 144, 159, 246
BstAFI CTTAAG 1 cut(s) 1837
BstAUI TGTACA 1 cut(s) 1347
BstBI TTCGAA 1 cut(s) 1393
BstC8I GCNNGC 2 cut(s) 1524, 1752
BstDEI CTNAG 9 cut(s) 194, 291, 435, 583, 709, 746, 1197, 1581, 2019
BstDSI CCRYGG 1 cut(s) 1466
BstENI CCTNNNNNAGG 1 cut(s) 623
BstF5I GGATG 6 cut(s) 83, 487, 1546, 1744, 1954, 1979
BstFNI CGCG 1 cut(s) 323
BstH2I RGCGCY 3 cut(s) 201, 249, 355
BstHHI GCGC 5 cut(s) 131, 200, 248, 354, 1302
BstKTI GATC 8 cut(s) 505, 591, 723, 775, 979, 1051, 1708, 2068
BstMAI GTCTC 2 cut(s) 1311, 1905
BstMBI GATC 8 cut(s) 502, 588, 720, 772, 976, 1048, 1705, 2065
BstMCI CGRYCG 2 cut(s) 455, 1561
BstMWI GCNNNNNNNGC 4 cut(s) 111, 206, 1308, 1592
BstNI CCWGG 3 cut(s) 640, 687, 1059
BstSCI CCNGG 3 cut(s) 638, 685, 1057
BstSFI CTRYAG 5 cut(s) 603, 609, 826, 1233, 1520
BstUI CGCG 1 cut(s) 323
BstV1I GCAGC 8 cut(s) 367, 595, 623, 685, 1777, 1967, 2080, 2083
BstV2I GAAGAC 1 cut(s) 1830
BstX2I RGATCY 3 cut(s) 588, 1048, 1705
BstYI RGATCY 3 cut(s) 588, 1048, 1705
BstZ17I GTATAC 3 cut(s) 878, 1367, 1781
BsuRI GGCC 4 cut(s) 209, 440, 685, 1526
BtgI CCRYGG 1 cut(s) 1466
BtgZI GCGATG 1 cut(s) 139
BtsCI GGATG 6 cut(s) 83, 487, 1546, 1744, 1954, 1979
BveI ACCTGC 1 cut(s) 728
Cac8I GCNNGC 2 cut(s) 1524, 1752
CaiI CAGNNNCTG 1 cut(s) 961
CfoI GCGC 5 cut(s) 131, 200, 248, 354, 1302
Cfr10I RCCGGY 2 cut(s) 255, 415
Cfr13I GGNCC 2 cut(s) 413, 1525
CpoI CGGWCCG 1 cut(s) 413
CseI GACGC 1 cut(s) 167
Csp6I GTAC 5 cut(s) 651, 692, 1003, 1074, 1348
CspI CGGWCCG 1 cut(s) 413
CviAII CATG 3 cut(s) 1043, 1345, 1467
CviQI GTAC 5 cut(s) 651, 692, 1003, 1074, 1348
DdeI CTNAG 9 cut(s) 194, 291, 435, 583, 709, 746, 1197, 1581, 2019
DinI GGCGCC 1 cut(s) 247
DpnI GATC 8 cut(s) 504, 590, 722, 774, 978, 1050, 1707, 2067
DpnII GATC 8 cut(s) 502, 588, 720, 772, 976, 1048, 1705, 2065
Eam1104I CTCTTC 2 cut(s) 85, 567
EarI CTCTTC 2 cut(s) 85, 567
EciI GGCGGA 2 cut(s) 269, 274
Ecl136II GAGCTC 3 cut(s) 193, 487, 1507
Eco130I CCWWGG 2 cut(s) 619, 1466
Eco24I GRGCYC 3 cut(s) 195, 489, 1509
Eco31I GGTCTC 1 cut(s) 1311
Eco32I GATATC 1 cut(s) 232
Eco47I GGWCC 1 cut(s) 413
Eco47III AGCGCT 1 cut(s) 353
Eco53kI GAGCTC 3 cut(s) 193, 487, 1507
Eco57I CTGAAG 2 cut(s) 761, 855
Eco88I CYCGRG 1 cut(s) 203
EcoICRI GAGCTC 3 cut(s) 193, 487, 1507
EcoNI CCTNNNNNAGG 1 cut(s) 623
EcoO109I RGGNCCY 1 cut(s) 1525
EcoRII CCWGG 3 cut(s) 638, 685, 1057
EcoRV GATATC 1 cut(s) 232
EcoT14I CCWWGG 2 cut(s) 619, 1466
EcoT38I GRGCYC 3 cut(s) 195, 489, 1509
EgeI GGCGCC 1 cut(s) 247
EheI GGCGCC 1 cut(s) 247
ErhI CCWWGG 2 cut(s) 619, 1466
Esp3I CGTCTC 1 cut(s) 1905
FaeI CATG 3 cut(s) 1046, 1348, 1470
FalI AAGNNNNNCTT 4 cut(s) 627, 659, 1768, 1800
FaqI GGGAC 2 cut(s) 669, 1012
FatI CATG 3 cut(s) 1042, 1344, 1466
FauI CCCGC 2 cut(s) 104, 110
FblI GTMKAC 4 cut(s) 742, 877, 1366, 1780
Fnu4HI GCNGC 9 cut(s) 132, 381, 609, 612, 699, 1791, 1956, 2094, 2097
FokI GGATG 6 cut(s) 70, 494, 1553, 1751, 1961, 1966
FriOI GRGCYC 3 cut(s) 195, 489, 1509
Fsp4HI GCNGC 9 cut(s) 132, 381, 609, 612, 699, 1791, 1956, 2094, 2097
FspBI CTAG 4 cut(s) 734, 1565, 1596, 1644
FspI TGCGCA 1 cut(s) 1301
GlaI GCGC 5 cut(s) 130, 199, 247, 353, 1301
GluI GCNGC 9 cut(s) 132, 381, 609, 612, 699, 1791, 1956, 2094, 2097
GsaI CCCAGC 1 cut(s) 2063
HaeII RGCGCY 3 cut(s) 201, 249, 355
HaeIII GGCC 4 cut(s) 209, 440, 685, 1526
HapII CCGG 7 cut(s) 70, 135, 256, 416, 455, 596, 896
HgaI GACGC 1 cut(s) 167
HhaI GCGC 5 cut(s) 131, 200, 248, 354, 1302
Hin1I GRCGYC 3 cut(s) 144, 159, 246
Hin1II CATG 3 cut(s) 1046, 1348, 1470
Hin6I GCGC 5 cut(s) 129, 198, 246, 352, 1300
HinP1I GCGC 5 cut(s) 129, 198, 246, 352, 1300
HincII GTYRAC 1 cut(s) 1288
HindII GTYRAC 1 cut(s) 1288
HindIII AAGCTT 1 cut(s) 1155
HinfI GANTC 9 cut(s) 4, 271, 285, 855, 1035, 1568, 1763, 1930, 2033
HpaII CCGG 7 cut(s) 70, 135, 256, 416, 455, 596, 896
HphI GGTGA 3 cut(s) 157, 585, 1130
Hpy166II GTNNAC 7 cut(s) 563, 743, 878, 1174, 1288, 1367, 1781
Hpy8I GTNNAC 7 cut(s) 563, 743, 878, 1174, 1288, 1367, 1781
Hpy99I CGWCG 7 cut(s) 111, 114, 146, 149, 152, 161, 1854
HpyCH4III ACNGT 4 cut(s) 471, 872, 1178, 1562
HpyCH4IV ACGT 2 cut(s) 144, 1001
HpyF10VI GCNNNNNNNGC 4 cut(s) 111, 206, 1308, 1592
HpyF3I CTNAG 9 cut(s) 194, 291, 435, 583, 709, 746, 1197, 1581, 2019
HpySE526I ACGT 2 cut(s) 144, 1001
Hsp92I GRCGYC 3 cut(s) 144, 159, 246
Hsp92II CATG 3 cut(s) 1046, 1348, 1470
HspAI GCGC 5 cut(s) 129, 198, 246, 352, 1300
KasI GGCGCC 1 cut(s) 245
Kpn2I TCCGGA 1 cut(s) 895
Kzo9I GATC 8 cut(s) 502, 588, 720, 772, 976, 1048, 1705, 2065
LguI GCTCTTC 1 cut(s) 567
LmnI GCTCC 3 cut(s) 119, 349, 1337
Lsp1109I GCAGC 8 cut(s) 367, 595, 623, 685, 1777, 1967, 2080, 2083
LweI GCATC 5 cut(s) 116, 809, 1624, 1729, 1988
MaeI CTAG 4 cut(s) 734, 1565, 1596, 1644
MaeII ACGT 2 cut(s) 144, 1001
MaeIII GTNAC 2 cut(s) 1207, 1249
MalI GATC 8 cut(s) 504, 590, 722, 774, 978, 1050, 1707, 2067
MboI GATC 8 cut(s) 502, 588, 720, 772, 976, 1048, 1705, 2065
MflI RGATCY 3 cut(s) 588, 1048, 1705
MhlI GDGCHC 3 cut(s) 195, 489, 1509
MluCI AATT 9 cut(s) 429, 761, 1121, 1358, 1639, 1721, 1905, 1992, 2082
Mly113I GGCGCC 1 cut(s) 246
MlyI GAGTC 3 cut(s) 279, 1577, 1757
MroI TCCGGA 1 cut(s) 895
MseI TTAA 8 cut(s) 764, 1125, 1262, 1487, 1728, 1838, 2052, 2081
MslI CAYNNNNRTG 1 cut(s) 1465
MspA1I CMGCKG 1 cut(s) 2063
MspCI CTTAAG 1 cut(s) 1837
MspI CCGG 7 cut(s) 70, 135, 256, 416, 455, 596, 896
MspR9I CCNGG 3 cut(s) 640, 687, 1059
Mva1269I GAATGC 1 cut(s) 1285
MvaI CCWGG 3 cut(s) 640, 687, 1059
MvnI CGCG 1 cut(s) 323
MwoI GCNNNNNNNGC 4 cut(s) 111, 206, 1308, 1592
NarI GGCGCC 1 cut(s) 246
NcoI CCATGG 1 cut(s) 1466
NdeII GATC 8 cut(s) 502, 588, 720, 772, 976, 1048, 1705, 2065
NlaIII CATG 3 cut(s) 1046, 1348, 1470
NlaIV GGNNCC 4 cut(s) 247, 600, 894, 1339
NmeAIII GCCGAG 1 cut(s) 409
NsbI TGCGCA 1 cut(s) 1301
NspV TTCGAA 1 cut(s) 1393
PciSI GCTCTTC 1 cut(s) 567
PcsI WCGNNNNNNNCGW 3 cut(s) 147, 153, 1858
PctI GAATGC 1 cut(s) 1285
PfeI GAWTC 6 cut(s) 4, 271, 855, 1035, 1930, 2033
Pfl23II CGTACG 1 cut(s) 650
PflFI GACNNNGTC 1 cut(s) 146
PflMI CCANNNNNTGG 1 cut(s) 1058
PkrI GCNGC 9 cut(s) 133, 382, 610, 613, 700, 1792, 1957, 2095, 2098
PleI GAGTC 3 cut(s) 279, 1576, 1757
PluTI GGCGCC 1 cut(s) 249
PpsI GAGTC 3 cut(s) 279, 1576, 1757
PshBI ATTAAT 1 cut(s) 2081
Psp124BI GAGCTC 3 cut(s) 195, 489, 1509
Psp6I CCWGG 3 cut(s) 638, 685, 1057
PspFI CCCAGC 1 cut(s) 2059
PspGI CCWGG 3 cut(s) 638, 685, 1057
PspLI CGTACG 1 cut(s) 650
PspN4I GGNNCC 4 cut(s) 247, 600, 894, 1339
PspPI GGNCC 2 cut(s) 413, 1525
PsrI GAACNNNNNNTAC 2 cut(s) 546, 578
PstI CTGCAG 3 cut(s) 613, 1237, 1524
PstNI CAGNNNCTG 1 cut(s) 961
PsuI RGATCY 3 cut(s) 588, 1048, 1705
PsyI GACNNNGTC 1 cut(s) 146
PvuII CAGCTG 1 cut(s) 2063
RsaI GTAC 5 cut(s) 652, 693, 1004, 1075, 1349
RsaNI GTAC 5 cut(s) 651, 692, 1003, 1074, 1348
RseI CAYNNNNRTG 1 cut(s) 1465
Rsr2I CGGWCCG 1 cut(s) 413
RsrII CGGWCCG 1 cut(s) 413
SacI GAGCTC 3 cut(s) 195, 489, 1509
SapI GCTCTTC 1 cut(s) 567
SaqAI TTAA 8 cut(s) 764, 1125, 1262, 1487, 1728, 1838, 2052, 2081
SatI GCNGC 9 cut(s) 132, 381, 609, 612, 699, 1791, 1956, 2094, 2097
Sau3AI GATC 8 cut(s) 502, 588, 720, 772, 976, 1048, 1705, 2065
Sau96I GGNCC 2 cut(s) 413, 1525
SbfI CCTGCAGG 1 cut(s) 1524
SchI GAGTC 3 cut(s) 279, 1577, 1757
ScrFI CCNGG 3 cut(s) 640, 687, 1059
SdaI CCTGCAGG 1 cut(s) 1524
SduI GDGCHC 3 cut(s) 195, 489, 1509
SfaNI GCATC 5 cut(s) 116, 809, 1624, 1729, 1988
SfcI CTRYAG 5 cut(s) 603, 609, 826, 1233, 1520
SfoI GGCGCC 1 cut(s) 247
SfuI TTCGAA 1 cut(s) 1393
SinI GGWCC 1 cut(s) 413
SmiMI CAYNNNNRTG 1 cut(s) 1465
SmlI CTYRAG 2 cut(s) 1508, 1837
SmoI CTYRAG 2 cut(s) 1508, 1837
Sse8387I CCTGCAGG 1 cut(s) 1524
Sse9I AATT 9 cut(s) 429, 761, 1121, 1358, 1639, 1721, 1905, 1992, 2082
SspDI GGCGCC 1 cut(s) 245
SspI AATATT 2 cut(s) 397, 1306
SspMI CTAG 4 cut(s) 734, 1565, 1596, 1644
SstI GAGCTC 3 cut(s) 195, 489, 1509
StyD4I CCNGG 3 cut(s) 638, 685, 1057
StyI CCWWGG 2 cut(s) 619, 1466
TaaI ACNGT 4 cut(s) 471, 872, 1178, 1562
TaiI ACGT 2 cut(s) 147, 1004
TaqII GACCGA 1 cut(s) 375
TasI AATT 9 cut(s) 429, 761, 1121, 1358, 1639, 1721, 1905, 1992, 2082
TatI WGTACW 2 cut(s) 691, 1347
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 6 cut(s) 4, 271, 855, 1035, 1930, 2033
Tru1I TTAA 8 cut(s) 764, 1125, 1262, 1487, 1728, 1838, 2052, 2081
Tru9I TTAA 8 cut(s) 764, 1125, 1262, 1487, 1728, 1838, 2052, 2081
TseI GCWGC 8 cut(s) 380, 608, 611, 698, 1790, 1955, 2093, 2096
TspDTI ATGAA 6 cut(s) 72, 807, 1836, 1902, 1955, 1965
TspGWI ACGGA 5 cut(s) 362, 539, 1580, 1838, 1972
Tth111I GACNNNGTC 1 cut(s) 146
Van91I CCANNNNNTGG 1 cut(s) 1058
Vha464I CTTAAG 1 cut(s) 1837
VpaK11BI GGWCC 1 cut(s) 413
VspI ATTAAT 1 cut(s) 2081
XagI CCTNNNNNAGG 1 cut(s) 623
XapI RAATTY 4 cut(s) 1121, 1358, 1721, 1992
XbaI TCTAGA 1 cut(s) 1564
XmiI GTMKAC 4 cut(s) 742, 877, 1366, 1780
XspI CTAG 4 cut(s) 734, 1565, 1596, 1644
ZraI GACGTC 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.