Rw6G016490

AarF domain-containing protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0009372
Physical Location & Seq
Forward (+)
32572522 .. 32577047
4526 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G016490.1

Sequence Viewer

Length: 1086 bp
ATGCTTCTTCGCCTTCCTTCACCTTCTCTCTCTTCTCTCCTTTCCACTCCCACTCCCAAACCCTCTCGATTCTCTCCGGCTCGGCCCCGCCGCTCGCTTTTCCGATGCGCCGCCCTCAAAAACTACGTCGACGTCGACATCGACGCCTTCACGGCCAAGTCCGGCTACCTCTTCGACCTCAGCGCGTCGGAGGCCAACTCCTTGGCCGACTATGATATCTCCAAAATTGGCGCCATTTACCGCCGGAAGCCTCTCGTTGTTTTCCGTCGGCTCCTACAGATCGCCCTCACTTTCGGCTACTGGTTTGCGCTTCGCTTCATTGATGCTCAAATGGAACTCTCCGACCAAATGTTCGAGGTTAGAGCTGCTGAGCTTCGGCAAATACTAGTAGAACTTGGACCGGCGTATGTCAAAATCGCTCAGGCTATTTCGTCTCGACCTGACTTGATACCACCATCTTACGTGGATGAACTCTCACTGTTGCAAGATCAAATATCTCCATTTTCTAATGAAGATGCTATAAAGACAATAGAAAATGAACTTGCTTTACCAATAGACGAGCTTTTCTCTGAGATCTCACCAGAACCTGTAGCTGCAGCTTCTCTTGGACAGGTGTATCAAGCCAGGCTTCGCCGTAGGGGACAGCTTGTTGCAGTTAAAGTACAAAGGCCTGGAGTACAAGCTGCTATTTCCTTAGACATATTGATCTTGCGTTCTCTAGCAGGTCTTCTCAGGAGAATAAGAAAATTAAACACTGATCTTCAGGCAGTTGTTGATGAATGGGCATCAAGTCTCTTTCGGGAGATGGACTACAGGAAGGAAGCAAATAATGGTATCAAGTTCAGACAGTTATATGGTGGTATACCTGATGTTTTGGTTCCAGAAATGTATCTGGAACACACAACTCGTAGGGTGCTTGTGATGGAGTGGGTGGAGGGACAGAAGCTGTCAGAAATGAATGATCTTTACTTGGTGGAGGTTGGGACTTACTGCTCTTTCAATCAGCTTCTGGAATGTGGTTTCTATCATGCAGACCACACCCTGGAAACCTTCTTCGCACATATGATGGAAAACTTGCTTATTTAG

Protein Analysis

361

Amino Acids

41.0

Weight (kDa)

5.75

Isoelectric Point (pI)

46.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 161 - 346 1.5e-51 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 135
Acc36I ACCTGC 1 cut(s) 713
AccB1I GGYRCC 1 cut(s) 230
AccB7I CCANNNNNTGG 1 cut(s) 1042
AccBSI CCGCTC 1 cut(s) 93
AccI GTMKAC 3 cut(s) 129, 135, 862
AccII CGCG 1 cut(s) 185
AciI CCGC 4 cut(s) 88, 91, 111, 241
AcoI YGGCCR 2 cut(s) 153, 204
AcuI CTGAAG 1 cut(s) 746
AcyI GRCGYC 3 cut(s) 132, 144, 231
AfaI GTAC 2 cut(s) 663, 678
AfiI CCNNNNNNNGG 1 cut(s) 1042
AgsI TTSAA 1 cut(s) 1000
AhlI ACTAGT 1 cut(s) 385
AjnI CCWGG 3 cut(s) 623, 670, 1041
AluBI AGCT 9 cut(s) 365, 373, 562, 593, 599, 646, 683, 946, 1006
AluI AGCT 9 cut(s) 365, 373, 562, 593, 599, 646, 683, 946, 1006
Alw26I GTCTC 2 cut(s) 438, 797
AlwNI CAGNNNCTG 3 cut(s) 587, 946, 1009
AoxI GGCC 5 cut(s) 83, 153, 192, 204, 668
ApeKI GCWGC 4 cut(s) 365, 593, 596, 683
ArsI GACNNNNNNTTYG 4 cut(s) 111, 143, 335, 367
AspLEI GCGC 4 cut(s) 110, 185, 233, 310
AspS9I GGNCC 2 cut(s) 84, 398
AsuHPI GGTGA 2 cut(s) 12, 570
AvaII GGWCC 1 cut(s) 398
BaeI ACNNNNGTAYC 2 cut(s) 599, 632
BanI GGYRCC 1 cut(s) 230
BbsI GAAGAC 1 cut(s) 719
BbvCI CCTCAGC 1 cut(s) 179
BbvI GCAGC 4 cut(s) 352, 580, 608, 670
BccI CCATC 4 cut(s) 463, 799, 916, 1060
BceAI ACGGC 2 cut(s) 168, 618
BciT130I CCWGG 3 cut(s) 625, 672, 1043
BcoDI GTCTC 2 cut(s) 438, 797
BcuI ACTAGT 1 cut(s) 385
BfaI CTAG 2 cut(s) 386, 719
BfmI CTRYAG 4 cut(s) 275, 588, 594, 811
BfoI RGCGCY 1 cut(s) 234
BfuAI ACCTGC 1 cut(s) 713
BglI GCCNNNNNGGC 1 cut(s) 152
BglII AGATCT 1 cut(s) 573
BisI GCNGC 6 cut(s) 91, 111, 366, 594, 597, 684
BlpI GCTNAGC 1 cut(s) 369
BlsI GCNGC 6 cut(s) 92, 112, 367, 595, 598, 685
Bme1390I CCNGG 3 cut(s) 625, 672, 1043
Bme18I GGWCC 1 cut(s) 398
BmgT120I GGNCC 2 cut(s) 84, 398
BmiI GGNNCC 4 cut(s) 86, 232, 272, 879
BmrFI CCNGG 3 cut(s) 625, 672, 1043
BmsI GCATC 4 cut(s) 95, 313, 505, 794
BpiI GAAGAC 1 cut(s) 719
BplI GAGNNNNNCTC 4 cut(s) 182, 214, 551, 583
BpmI CTGGAG 1 cut(s) 693
Bpu10I CCTNAGC 2 cut(s) 179, 420
Bpu1102I GCTNAGC 1 cut(s) 369
BsaAI YACGTR 1 cut(s) 463
BsaHI GRCGYC 3 cut(s) 132, 144, 231
BsaJI CCNNGG 2 cut(s) 201, 1041
BsaXI ACNNNNNCTCC 2 cut(s) 37, 67
Bsc4I CCNNNNNNNGG 1 cut(s) 1042
Bse118I RCCGGY 1 cut(s) 400
Bse1I ACTGG 1 cut(s) 305
BseBI CCWGG 3 cut(s) 625, 672, 1043
BseDI CCNNGG 2 cut(s) 201, 1041
BseGI GGATG 1 cut(s) 472
BseLI CCNNNNNNNGG 1 cut(s) 1042
BseMII CTCAG 5 cut(s) 193, 360, 434, 561, 745
BseNI ACTGG 1 cut(s) 305
BseXI GCAGC 4 cut(s) 352, 580, 608, 670
Bsh1236I CGCG 1 cut(s) 185
BshFI GGCC 5 cut(s) 85, 155, 194, 206, 670
BshNI GGYRCC 1 cut(s) 230
BsiSI CCGG 4 cut(s) 77, 162, 244, 401
BslFI GGGAC 3 cut(s) 654, 951, 997
BslI CCNNNNNNNGG 1 cut(s) 1042
BsmAI GTCTC 2 cut(s) 438, 797
BsmBI CGTCTC 1 cut(s) 438
BsmFI GGGAC 3 cut(s) 654, 951, 997
BsnI GGCC 5 cut(s) 85, 155, 194, 206, 670
Bsp143I GATC 6 cut(s) 279, 487, 573, 705, 757, 961
Bsp1720I GCTNAGC 1 cut(s) 369
BspACI CCGC 4 cut(s) 88, 91, 111, 241
BspANI GGCC 5 cut(s) 85, 155, 194, 206, 670
BspCNI CTCAG 5 cut(s) 192, 361, 433, 562, 744
BspFNI CGCG 1 cut(s) 185
BspLI GGNNCC 4 cut(s) 86, 232, 272, 879
BspMAI CTGCAG 1 cut(s) 598
BspMI ACCTGC 1 cut(s) 713
BspT107I GGYRCC 1 cut(s) 230
BsrBI CCGCTC 1 cut(s) 93
BsrFI RCCGGY 1 cut(s) 400
BsrI ACTGG 1 cut(s) 305
BssAI RCCGGY 1 cut(s) 400
BssECI CCNNGG 2 cut(s) 201, 1041
BssMI GATC 6 cut(s) 279, 487, 573, 705, 757, 961
BssNAI GTATAC 1 cut(s) 863
BssNI GRCGYC 3 cut(s) 132, 144, 231
BssT1I CCWWGG 1 cut(s) 201
Bst1107I GTATAC 1 cut(s) 863
Bst2UI CCWGG 3 cut(s) 625, 672, 1043
Bst4CI ACNGT 2 cut(s) 480, 849
Bst6I CTCTTC 2 cut(s) 37, 176
BstACI GRCGYC 3 cut(s) 132, 144, 231
BstBAI YACGTR 1 cut(s) 463
BstC8I GCNNGC 1 cut(s) 95
BstDEI CTNAG 6 cut(s) 179, 369, 420, 570, 694, 731
BstF5I GGATG 1 cut(s) 472
BstFNI CGCG 1 cut(s) 185
BstH2I RGCGCY 1 cut(s) 234
BstHHI GCGC 4 cut(s) 110, 185, 233, 310
BstKTI GATC 6 cut(s) 282, 490, 576, 708, 760, 964
BstMAI GTCTC 2 cut(s) 438, 797
BstMBI GATC 6 cut(s) 279, 487, 573, 705, 757, 961
BstMWI GCNNNNNNNGC 2 cut(s) 152, 191
BstNI CCWGG 3 cut(s) 625, 672, 1043
BstSCI CCNGG 3 cut(s) 623, 670, 1041
BstSFI CTRYAG 4 cut(s) 275, 588, 594, 811
BstUI CGCG 1 cut(s) 185
BstV1I GCAGC 4 cut(s) 352, 580, 608, 670
BstV2I GAAGAC 1 cut(s) 719
BstX2I RGATCY 1 cut(s) 573
BstXI CCANNNNNNTGG 1 cut(s) 202
BstYI RGATCY 1 cut(s) 573
BstZ17I GTATAC 1 cut(s) 863
BsuRI GGCC 5 cut(s) 85, 155, 194, 206, 670
BtsCI GGATG 1 cut(s) 472
BtsIMutI CAGTG 2 cut(s) 476, 753
BveI ACCTGC 1 cut(s) 713
Cac8I GCNNGC 1 cut(s) 95
CaiI CAGNNNCTG 3 cut(s) 587, 946, 1009
CfoI GCGC 4 cut(s) 110, 185, 233, 310
Cfr10I RCCGGY 1 cut(s) 400
Cfr13I GGNCC 2 cut(s) 84, 398
CseI GACGC 2 cut(s) 152, 174
Csp6I GTAC 2 cut(s) 662, 677
CviAII CATG 1 cut(s) 1028
CviQI GTAC 2 cut(s) 662, 677
DdeI CTNAG 6 cut(s) 179, 369, 420, 570, 694, 731
DinI GGCGCC 1 cut(s) 232
DpnI GATC 6 cut(s) 281, 489, 575, 707, 759, 963
DpnII GATC 6 cut(s) 279, 487, 573, 705, 757, 961
EaeI YGGCCR 2 cut(s) 153, 204
Eam1104I CTCTTC 2 cut(s) 37, 176
EarI CTCTTC 2 cut(s) 37, 176
Eco130I CCWWGG 1 cut(s) 201
Eco147I AGGCCT 1 cut(s) 670
Eco32I GATATC 1 cut(s) 217
Eco47I GGWCC 1 cut(s) 398
Eco57I CTGAAG 1 cut(s) 746
EcoRII CCWGG 3 cut(s) 623, 670, 1041
EcoRV GATATC 1 cut(s) 217
EcoT14I CCWWGG 1 cut(s) 201
EgeI GGCGCC 1 cut(s) 232
EheI GGCGCC 1 cut(s) 232
ErhI CCWWGG 1 cut(s) 201
Esp3I CGTCTC 1 cut(s) 438
FaeI CATG 1 cut(s) 1031
FalI AAGNNNNNCTT 2 cut(s) 612, 644
FaqI GGGAC 3 cut(s) 654, 951, 997
FatI CATG 1 cut(s) 1027
FauI CCCGC 1 cut(s) 95
FauNDI CATATG 1 cut(s) 1062
FblI GTMKAC 3 cut(s) 129, 135, 862
Fnu4HI GCNGC 6 cut(s) 91, 111, 366, 594, 597, 684
FokI GGATG 1 cut(s) 479
Fsp4HI GCNGC 6 cut(s) 91, 111, 366, 594, 597, 684
FspBI CTAG 2 cut(s) 386, 719
GlaI GCGC 4 cut(s) 109, 184, 232, 309
GluI GCNGC 6 cut(s) 91, 111, 366, 594, 597, 684
GsuI CTGGAG 1 cut(s) 693
HaeII RGCGCY 1 cut(s) 234
HaeIII GGCC 5 cut(s) 85, 155, 194, 206, 670
HapII CCGG 4 cut(s) 77, 162, 244, 401
HgaI GACGC 2 cut(s) 152, 174
HhaI GCGC 4 cut(s) 110, 185, 233, 310
Hin1I GRCGYC 3 cut(s) 132, 144, 231
Hin1II CATG 1 cut(s) 1031
Hin6I GCGC 4 cut(s) 108, 183, 231, 308
HinP1I GCGC 4 cut(s) 108, 183, 231, 308
HincII GTYRAC 2 cut(s) 130, 136
HindII GTYRAC 2 cut(s) 130, 136
HinfI GANTC 1 cut(s) 69
HpaII CCGG 4 cut(s) 77, 162, 244, 401
HphI GGTGA 2 cut(s) 12, 570
Hpy166II GTNNAC 3 cut(s) 130, 136, 863
Hpy188I TCNGA 6 cut(s) 104, 190, 343, 571, 845, 952
Hpy188III TCNNGA 7 cut(s) 66, 435, 733, 800, 881, 893, 1010
Hpy8I GTNNAC 3 cut(s) 130, 136, 863
Hpy99I CGWCG 6 cut(s) 131, 134, 137, 146, 190, 270
HpyAV CCTTC 6 cut(s) 23, 27, 33, 157, 811, 1060
HpyCH4III ACNGT 2 cut(s) 480, 849
HpyCH4IV ACGT 3 cut(s) 126, 132, 462
HpyCH4V TGCA 4 cut(s) 484, 596, 653, 1031
HpyF10VI GCNNNNNNNGC 2 cut(s) 152, 191
HpyF3I CTNAG 6 cut(s) 179, 369, 420, 570, 694, 731
HpySE526I ACGT 3 cut(s) 126, 132, 462
Hsp92I GRCGYC 3 cut(s) 132, 144, 231
Hsp92II CATG 1 cut(s) 1031
HspAI GCGC 4 cut(s) 108, 183, 231, 308
KasI GGCGCC 1 cut(s) 230
Kzo9I GATC 6 cut(s) 279, 487, 573, 705, 757, 961
LmnI GCTCC 1 cut(s) 276
Lsp1109I GCAGC 4 cut(s) 352, 580, 608, 670
LweI GCATC 4 cut(s) 95, 313, 505, 794
MaeI CTAG 2 cut(s) 386, 719
MaeII ACGT 3 cut(s) 126, 132, 462
MalI GATC 6 cut(s) 281, 489, 575, 707, 759, 963
MbiI CCGCTC 1 cut(s) 93
MboI GATC 6 cut(s) 279, 487, 573, 705, 757, 961
MboII GAAGA 6 cut(s) 24, 163, 524, 719, 752, 1045
MflI RGATCY 1 cut(s) 573
MluCI AATT 2 cut(s) 225, 746
Mly113I GGCGCC 1 cut(s) 231
MmeI TCCRAC 2 cut(s) 168, 366
MseI TTAA 2 cut(s) 657, 749
MspI CCGG 4 cut(s) 77, 162, 244, 401
MspR9I CCNGG 3 cut(s) 625, 672, 1043
MvaI CCWGG 3 cut(s) 625, 672, 1043
MvnI CGCG 1 cut(s) 185
MwoI GCNNNNNNNGC 2 cut(s) 152, 191
NarI GGCGCC 1 cut(s) 231
NdeI CATATG 1 cut(s) 1062
NdeII GATC 6 cut(s) 279, 487, 573, 705, 757, 961
NlaIII CATG 1 cut(s) 1031
NlaIV GGNNCC 4 cut(s) 86, 232, 272, 879
NmeAIII GCCGAG 1 cut(s) 61
PceI AGGCCT 1 cut(s) 670
PcsI WCGNNNNNNNCGW 2 cut(s) 132, 138
PfeI GAWTC 1 cut(s) 69
PflMI CCANNNNNTGG 1 cut(s) 1042
PkrI GCNGC 6 cut(s) 92, 112, 367, 595, 598, 685
PluTI GGCGCC 1 cut(s) 234
Ppu21I YACGTR 1 cut(s) 463
Psp6I CCWGG 3 cut(s) 623, 670, 1041
PspGI CCWGG 3 cut(s) 623, 670, 1041
PspN4I GGNNCC 4 cut(s) 86, 232, 272, 879
PspPI GGNCC 2 cut(s) 84, 398
PsrI GAACNNNNNNTAC 2 cut(s) 531, 563
PstI CTGCAG 1 cut(s) 598
PstNI CAGNNNCTG 3 cut(s) 587, 946, 1009
PsuI RGATCY 1 cut(s) 573
RsaI GTAC 2 cut(s) 663, 678
RsaNI GTAC 2 cut(s) 662, 677
SalI GTCGAC 2 cut(s) 128, 134
SaqAI TTAA 2 cut(s) 657, 749
SatI GCNGC 6 cut(s) 91, 111, 366, 594, 597, 684
Sau3AI GATC 6 cut(s) 279, 487, 573, 705, 757, 961
Sau96I GGNCC 2 cut(s) 84, 398
ScrFI CCNGG 3 cut(s) 625, 672, 1043
SfaNI GCATC 4 cut(s) 95, 313, 505, 794
SfcI CTRYAG 4 cut(s) 275, 588, 594, 811
SfoI GGCGCC 1 cut(s) 232
SgrDI CGTCGACG 1 cut(s) 128
SinI GGWCC 1 cut(s) 398
SpeI ACTAGT 1 cut(s) 385
Sse9I AATT 2 cut(s) 225, 746
SseBI AGGCCT 1 cut(s) 670
SsiI CCGC 4 cut(s) 88, 91, 111, 241
SspDI GGCGCC 1 cut(s) 230
SspMI CTAG 2 cut(s) 386, 719
StuI AGGCCT 1 cut(s) 670
StyD4I CCNGG 3 cut(s) 623, 670, 1041
StyI CCWWGG 1 cut(s) 201
TaaI ACNGT 2 cut(s) 480, 849
TaiI ACGT 3 cut(s) 129, 135, 465
TaqI TCGA 7 cut(s) 67, 129, 135, 141, 174, 354, 436
TasI AATT 2 cut(s) 225, 746
TatI WGTACW 2 cut(s) 661, 676
TauI GCSGC 2 cut(s) 93, 113
TfiI GAWTC 1 cut(s) 69
Tru1I TTAA 2 cut(s) 657, 749
Tru9I TTAA 2 cut(s) 657, 749
TscAI CASTG 2 cut(s) 483, 760
TseI GCWGC 4 cut(s) 365, 593, 596, 683
TspDTI ATGAA 6 cut(s) 307, 483, 525, 552, 792, 971
TspGWI ACGGA 1 cut(s) 254
TspRI CASTG 2 cut(s) 483, 760
Van91I CCANNNNNTGG 1 cut(s) 1042
VpaK11BI GGWCC 1 cut(s) 398
XmiI GTMKAC 3 cut(s) 129, 135, 862
XspI CTAG 2 cut(s) 386, 719
ZraI GACGTC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.