MD10G1093900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013889
Physical Location & Seq
Reverse (-)
14601695 .. 14602869
1175 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1093900.v1.1.491

Sequence Viewer

Length: 477 bp
ATGGATCCTAGAATCTCCTTCTCCAATGACTTTGCAGACACTCAACAGGCCATCAAGCACGAATCAAGCTACCGGGAAGCGCCCGTCTCATCGGATTTCGAATTCTCCGTGGACAACTTTGGTATGATCTCGGCGGACGAGATCATATTCAAGGGCACATTGTTGCCCTTAAAGGATAATGGTGCCAACAACCAGCTAGGGAATAAGACTACTACTTTGAGAGATGAGCTACTTGCTGATGATGAGGATTATGGGGATGTGTTGCCAAGAGCAACAAATAGTTTGAACAGATGGAAGGAGCGTTTGGGGCTCAAGAGGGCTCAAATTGTTCAAAAAAATGGCGATAGAGGTCATTCGGTTTTGGAGAGGATTGCTGAAGAGAGGAGGCCTGTGTTTGTTCATGAGGCTGTGCTTATTAGCAAGGAAAGCCAGGAATTTCTATTTGGAGGGTTCAATTGCAGAGATGATGGAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

159

Amino Acids

17.86

Weight (kDa)

4.91

Isoelectric Point (pI)

24.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013889)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19200 AT4G34419
fragaria_vesca FvH4_2g06210
malus_domestica MD05G1079600.v1.1 MD10G1093900.v1.1
prunus_persica Prupe.8G123500_v2.0.a1
pyrus_communis pycom10g07730
rosa_chinensis RchiOBHm_Chr6g0256981
rosa_laevigata RLG00000014667
rosa_multiflora Rmu_sc0000659.1_g000036
rosa_roxburghii Rroxscaffold_7G00208390
rosa_rugosa Rorug05G0575900
rosa_samantha Rh6AG094500 Rh6BG085800 Rh6CG081900 Rh6DG077600
rosa_wichuraiana Rw6G008090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 182
AciI CCGC 1 cut(s) 134
AclWI GGATC 1 cut(s) 12
AcsI RAATTY 2 cut(s) 101, 434
AcuI CTGAAG 1 cut(s) 396
AgsI TTSAA 4 cut(s) 151, 286, 332, 454
AjnI CCWGG 1 cut(s) 429
AjuI GAANNNNNNNTTGG 4 cut(s) 287, 319, 426, 458
AluBI AGCT 3 cut(s) 69, 196, 229
AluI AGCT 3 cut(s) 69, 196, 229
Alw26I GTCTC 1 cut(s) 91
AlwI GGATC 1 cut(s) 12
AoxI GGCC 2 cut(s) 48, 386
ApoI RAATTY 2 cut(s) 101, 434
ArsI GACNNNNNNTTYG 2 cut(s) 199, 231
AspLEI GCGC 1 cut(s) 82
AsuC2I CCSGG 1 cut(s) 74
AsuII TTCGAA 1 cut(s) 99
BaeGI GKGCMC 1 cut(s) 158
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 182
BanII GRGCYC 2 cut(s) 312, 322
BccI CCATC 3 cut(s) 59, 285, 461
BciT130I CCWGG 1 cut(s) 431
BcnI CCSGG 1 cut(s) 74
BcoDI GTCTC 1 cut(s) 91
BfaI CTAG 2 cut(s) 9, 197
BfoI RGCGCY 1 cut(s) 83
Bme1390I CCNGG 2 cut(s) 74, 431
BmiI GGNNCC 2 cut(s) 6, 184
BmrFI CCNGG 2 cut(s) 74, 431
Bpu14I TTCGAA 1 cut(s) 99
BpuEI CTTGAG 1 cut(s) 296
BpuMI CCSGG 1 cut(s) 74
BsaJI CCNNGG 1 cut(s) 108
BseBI CCWGG 1 cut(s) 431
BseDI CCNNGG 1 cut(s) 108
BseGI GGATG 1 cut(s) 262
BseRI GAGGAG 1 cut(s) 397
BseSI GKGCMC 1 cut(s) 158
BshFI GGCC 2 cut(s) 50, 388
BshNI GGYRCC 1 cut(s) 182
BsiSI CCGG 1 cut(s) 73
BsmAI GTCTC 1 cut(s) 91
BsmBI CGTCTC 1 cut(s) 91
BsnI GGCC 2 cut(s) 50, 388
Bsp119I TTCGAA 1 cut(s) 99
Bsp1286I GDGCHC 3 cut(s) 158, 312, 322
Bsp143I GATC 3 cut(s) 4, 126, 141
BspACI CCGC 1 cut(s) 134
BspANI GGCC 2 cut(s) 50, 388
BspHI TCATGA 1 cut(s) 400
BspLI GGNNCC 2 cut(s) 6, 184
BspPI GGATC 1 cut(s) 12
BspT104I TTCGAA 1 cut(s) 99
BspT107I GGYRCC 1 cut(s) 182
BssECI CCNNGG 1 cut(s) 108
BssMI GATC 3 cut(s) 4, 126, 141
Bst2UI CCWGG 1 cut(s) 431
Bst6I CTCTTC 1 cut(s) 372
BstBI TTCGAA 1 cut(s) 99
BstDSI CCRYGG 1 cut(s) 108
BstF5I GGATG 1 cut(s) 262
BstH2I RGCGCY 1 cut(s) 83
BstHHI GCGC 1 cut(s) 82
BstKTI GATC 3 cut(s) 7, 129, 144
BstMAI GTCTC 1 cut(s) 91
BstMBI GATC 3 cut(s) 4, 126, 141
BstMWI GCNNNNNNNGC 2 cut(s) 307, 426
BstNI CCWGG 1 cut(s) 431
BstSCI CCNGG 2 cut(s) 72, 429
BstSLI GKGCMC 1 cut(s) 158
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 2 cut(s) 50, 388
BtgI CCRYGG 1 cut(s) 108
BtsCI GGATG 1 cut(s) 262
CciI TCATGA 1 cut(s) 400
CfoI GCGC 1 cut(s) 82
CviAII CATG 1 cut(s) 401
CviJI RGCY 9 cut(s) 50, 69, 196, 229, 310, 320, 388, 407, 429
CviKI_1 RGCY 9 cut(s) 50, 69, 196, 229, 310, 320, 388, 407, 429
DpnI GATC 3 cut(s) 6, 128, 143
DpnII GATC 3 cut(s) 4, 126, 141
Eam1104I CTCTTC 1 cut(s) 372
EarI CTCTTC 1 cut(s) 372
EciI GGCGGA 1 cut(s) 149
Eco147I AGGCCT 1 cut(s) 388
Eco24I GRGCYC 2 cut(s) 312, 322
Eco57I CTGAAG 1 cut(s) 396
EcoRI GAATTC 1 cut(s) 101
EcoRII CCWGG 1 cut(s) 429
EcoT38I GRGCYC 2 cut(s) 312, 322
Esp3I CGTCTC 1 cut(s) 91
FaeI CATG 1 cut(s) 404
FaiI YATR 4 cut(s) 125, 146, 252, 402
FatI CATG 1 cut(s) 400
FokI GGATG 1 cut(s) 269
FriOI GRGCYC 2 cut(s) 312, 322
FspBI CTAG 2 cut(s) 9, 197
GlaI GCGC 1 cut(s) 81
HaeII RGCGCY 1 cut(s) 83
HaeIII GGCC 2 cut(s) 50, 388
HapII CCGG 1 cut(s) 73
HhaI GCGC 1 cut(s) 82
Hin1II CATG 1 cut(s) 404
Hin6I GCGC 1 cut(s) 80
HinP1I GCGC 1 cut(s) 80
HinfI GANTC 3 cut(s) 12, 62, 471
HpaII CCGG 1 cut(s) 73
Hpy166II GTNNAC 1 cut(s) 112
Hpy188I TCNGA 2 cut(s) 94, 476
Hpy188III TCNNGA 2 cut(s) 313, 401
Hpy8I GTNNAC 1 cut(s) 112
HpyAV CCTTC 2 cut(s) 28, 289
HpyCH4V TGCA 2 cut(s) 35, 459
HpyF10VI GCNNNNNNNGC 2 cut(s) 307, 426
Hsp92II CATG 1 cut(s) 404
HspAI GCGC 1 cut(s) 80
Kzo9I GATC 3 cut(s) 4, 126, 141
LmnI GCTCC 1 cut(s) 298
LpnPI CCDG 6 cut(s) 32, 86, 206, 402, 416, 443
MaeI CTAG 2 cut(s) 9, 197
MalI GATC 3 cut(s) 6, 128, 143
MboI GATC 3 cut(s) 4, 126, 141
MboII GAAGA 1 cut(s) 389
MfeI CAATTG 1 cut(s) 454
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 3 cut(s) 158, 312, 322
MluCI AATT 4 cut(s) 101, 324, 434, 454
MnlI CCTC 8 cut(s) 238, 309, 341, 360, 375, 378, 397, 440
MseI TTAA 1 cut(s) 170
MspI CCGG 1 cut(s) 73
MspR9I CCNGG 2 cut(s) 74, 431
MunI CAATTG 1 cut(s) 454
MvaI CCWGG 1 cut(s) 431
MwoI GCNNNNNNNGC 2 cut(s) 307, 426
NciI CCSGG 1 cut(s) 74
NdeII GATC 3 cut(s) 4, 126, 141
NlaIII CATG 1 cut(s) 404
NlaIV GGNNCC 2 cut(s) 6, 184
NmeAIII GCCGAG 1 cut(s) 110
NspV TTCGAA 1 cut(s) 99
PagI TCATGA 1 cut(s) 400
PceI AGGCCT 1 cut(s) 388
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PfeI GAWTC 3 cut(s) 12, 62, 471
Psp6I CCWGG 1 cut(s) 429
PspGI CCWGG 1 cut(s) 429
PspN4I GGNNCC 2 cut(s) 6, 184
PsuI RGATCY 1 cut(s) 4
SaqAI TTAA 1 cut(s) 170
Sau3AI GATC 3 cut(s) 4, 126, 141
ScrFI CCNGG 2 cut(s) 74, 431
SduI GDGCHC 3 cut(s) 158, 312, 322
SetI ASST 4 cut(s) 71, 198, 231, 352
SfuI TTCGAA 1 cut(s) 99
SmlI CTYRAG 1 cut(s) 311
SmoI CTYRAG 1 cut(s) 311
Sse9I AATT 4 cut(s) 101, 324, 434, 454
SseBI AGGCCT 1 cut(s) 388
SsiI CCGC 1 cut(s) 134
SspMI CTAG 2 cut(s) 9, 197
StuI AGGCCT 1 cut(s) 388
StyD4I CCNGG 2 cut(s) 72, 429
TaqI TCGA 1 cut(s) 99
TasI AATT 4 cut(s) 101, 324, 434, 454
TfiI GAWTC 3 cut(s) 12, 62, 471
Tru1I TTAA 1 cut(s) 170
Tru9I TTAA 1 cut(s) 170
TspDTI ATGAA 1 cut(s) 389
TspGWI ACGGA 1 cut(s) 97
XapI RAATTY 2 cut(s) 101, 434
XspI CTAG 2 cut(s) 9, 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.