Rorug05G0575900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0013889
Physical Location & Seq
Reverse (-)
77202879 .. 77210909
8031 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0575900.1

Sequence Viewer

Length: 6306 bp
ATGGCCTGGTTCTCTCGAAAAGCTGATGCTTCTCTTCCTTCATCAGCTGAACCACTATCAGCAGCTGGTGGGTGGAAATATGACGTGTATTTGAGTTTCAGGGGTGTAGATACTCGAAGGGGTTTTACATCCCATTTATACGATCAGCTGCAGAGGAGTGGAATTAAAACATTCATGGATGATCAAGATCTTCAAGTAGGGGATGATGTTTCTCCCACTCTCCTAAAGGCAATTGAAGGGGTTTTACATCGAAGGGGTTTTACATCCCATTTATACGATCGGCTGCAGAGGAGTGGAATTAAAACATTCATGGATGATCAAGATCTTCAAGTAGGGGATGATGTTTCTCCCACTCTCCTAAAGGCAATTGAAGGATCAAGGTTCGCAATTGTTGTTCTGTCCCAAAATTATGCTTCTTCTGCTTGGTGTTTGGATGAACTTACAAAGATTTGTCAGTTCATGGAGGACGGCCCTAGAATTCTTCCAGTTTTTTATAAGGTTGACCCTAGTCATGTAAGAAAACAAAAGGGGAGTTTTGAGGATGCTTTAGCCAAGCATGAAAGGTCTGGGCGATACGAATCGAAGAAGGTCCAGCAGTGGAGAGATGCTTTAAGAAAAGTGGCCGACTTTGCTGGGTTTGATACACAGAATTACAGGACTGATATAGATCTTCTCGAACACATTGTGGAAATTGTGTGCAGTAAAGTACAATCTGTTTTGGAAATCGAAAAAGTACAACCTATTGAAATCAACACAGGAGGTTTTGAATTATTTGAACCAACAAGAAAAGCCATGGATGACGTTATGGAGGCGCTAAAAGATGACGAGGTCACTGCCGTTGGTGTCTACGGCATGGCAGGCGTTGGAAAGACAGCCATGGTAAAACATGTCGGTGCACATGCCCGAAAAAGTGGAATTTTTCATCATGTGATTATCGGTGATGTATCCCAAAGCCCTGACTTGGAAACAATTCAAGGCACATTGGCAGGGCTGCTGGGCTACAAATTACATGAGAAGACAGAAATTGGCAGAGCAGCTAGATTGAGTGAGGAGATAAAGAGAAGGGGAAAGATCTTTATAATCCTGGACAACATTTGGGAGAGAATGGATTTGTCAGACATAGGAATACCTAGCTATAAGGAGCTTCAAAAGTGCAATTCCAAAGTCCTACTCACCACACGGAGAGAGAATGTGTGTCATGAGATGAGATGCCAAGCAATTATTCATCTCAATATCTTATCAGAACAAGATTCTTGGTCCTTGTTTGTGAAAACGGCAAGAATAGACTCTGAATCGATCGTTTTCTATGATGTAGCGAGGGAGGTGGTTAGTAAATGTGCTGGTCTACCGATTGCATTGATAACAGTTGCAAGGGCACTTGGAGGTAAAGAACTAGTGGAATGGAAAAGAGCAGCTCGAGCACTAGAGAAGTCGCAATCTGCCAACCCTGAACATGAAGAAGAGGCATTCAGATGTATAAAATTAAGCTATGATTACTTGAAAGATGAAGATACCAAGTCATGCTTCTTGCTTTGTTGCCTATTTCCTGAAGACTATGACATCCCAATAGGAGACTTGTTCAAGTATGCAATCGGGAAAGGATTGTTTCGGGATGCCGAGACAATCCAAGAAGCTAGAGCATCAGCTGATTCAGCCGTAAGGTACCTGAAACATTTTAGCTTGCTTTTGGATAGTGAGAAAAAGGGGTGTGTAAGGATGCATGATGTCATTCGGGATGCTGCCCTGAATATTGCAAAATCTGAAGACGGGCATGGGTTTTTGGTAAAAGCTGGCTGTGGTTTAAAAAGCTGGCCACTTCAATTACATGAAGGCTACTCTGCAATCTCATTAATGAACAATGATATCCACAAGCTGCCCGGAGAGCTGGTATGTCCAAAACTCCAGATTTTATTACTACAGCAGAATGCTGATTTAAATGAGATGCCAGGAACTTTTTTCCAAAGTCCTAATGAATTAAGGGTCTTAGACCTTAGCCTCACCGGCATTACAGAACTTCCTCAATCATTCAGTCTCCTAATGAACCTCCAAGCTTTATATCTAGATTTTTGCCAGAGACTGATTGACTTTTCCTTAATTGGAAAACTAAAGAAGCTTGAGATTCTTAGTATGAGAGGAAATGTTTTGAAAGAATTGTCGAGAGAAATAGGGAATCTGATCAATCTAAGGATGCTGGACATTACTGATGGAATTATTAGCAAAATTCCATCAAAAGTGATATCGCAGTTGCATAGATTAGAAGAACTGTACATGCCATTTCGATTTGGGAATTGGGTCAGTAAGGTTGAAGGAGAAGGAGAAGAAACCGATATTGGCTTTGGTGAGGTAACTGGCTTAACATGTCTAAATATTTTGAAGGTTTCCCTACCTGCAAAATGCATCCCTGAAGAGCTTGAGTTTTATCCCAATTGGTTGTTCTTTGATATTTGTATTAGTGGGGACACAAGCAGCAGAGACATTAAGAGAGACATAAGTAATCAAGGCTTTCAACACAGTCATAAAGATTCAAGATCCTTGAGTCTTGACACAAGAATTAGTGCCTTACCAGCTTGGTTTGTCCATGAGGTAACAGTCAAGACAGAGAAGCTACTGTACGTAAACTGCAAATGGTTAAATAACATTCTTGTTGAATATCACCATGGGAGGTTACATATGCTGAAACATCTCTCTATTATTGGTCCCAGTGAGAGCTTGGAAGAGCTGATGAATGCTATAACATGGGTTCCAGTTAAACCCGTGTTCGAGAACTTGGAAGAGTTGCATTTGGAGTGTGTGACTTCTTTGAAAGAGTTATGTGTTGGAAAATTACCACCTGGGTCTCTATACAATCTGAAGTTATTGAAGGTCCATTATTGTCCTAACTTGGGGAATATTCTTTTACCATCAAAATTGTTACATAGATTGCCAAATCTGGAGAACATAATCTGTAGGGATATGTCTAGACTGGAACACGTGTTTGGATGTCAAGGGTTTGAGCCAGAGCAATCAAAACTGAGAGAGATGACCTTTATCAATCTACCTGCTATAAGAAACATATGTAACGGTCCAGCTCCACGTGCAATGTTCCAAGCTCTTAAAAGTTTGTTCATTTATCATTGCAACTTTCTAGGATGTCTCTTCTCGTTTGATACTGCTCAAGTTCTTTTTCAATTGGAAGACTTTTTTGTAGACAGTTGCCCTTTATTGGAAAGACTAATTGAAGTACGCACGGAAAGAGTGAACAACAAGAAGATCATTCTTCCAAAATTGAAGAACTTGGGTTTAGAGAAACTTCCTATATTGTACAGTGGAGGTGCTACTATTGATATCGAGTGTCCTTCCTTGGAACACTTGTATGTGAAGGAGTGCCCCCAGTTTACGGCCTCTGCTTCTGACTTCCAGAGCAAGAACCAAGTCCAAGTCAACGATAAATTACATTACTTTGGTCTACTTGGCAGAAGGACTGAAGGACAACATATTGAAGCCATTGCGGAATCTGTGTTCAGTACATTACGACCTATTGAAACTGAGTTTACAATGCCCATGGGAGATTTTCAAGCATTTGAAGCAACAAGACAGGCCATGAATAAGGTTATGAAGGCACTAGAAGCTGATGAGGTCACAGCCATTGGGGTCTACGGAATGGGAGGAGTTGGAAAATCATCCATGGTTGAACATGTTTGTGCAGAAGTTCGGGATAATGGGATGTTTCATCAGGTGATTTCAGCTGTTGTATCCCAAAACCCTGACTTGAGAAAAATTCAAGGCACATTGGCAGATATGTTGGGCTTTATATTTGAGGAGGAGACAGAAATTGGAAGAGCTCGTAGATTGATGCAGAGGATAAGGAGAGGAAATAGGATCCTTGTAATCTTGGACAACATTTGGGAGAGAACAGATTTGTCAAACATAGGAATTCCCAGCTACTACGAGCTTCAAAGATGCAATTCCAAAGTCCTACTCATCACAAGGACACAGAATGTCTGTCGTGCCATGGAATGCCAATCAAACATTTATCTTGATGTCTTATCAGAAGAAGATTCTTGGACCTTATTTGTGAACAAAGCAAGAACGTCTTTTGAATCGACCAGTTTGTGTGATGTTGCAAAGAAGGTAGCTAGAGCATGTGCTGGTCTTCCAGTTGCGTTGATAGCAGTTGCGAAGGCACTTGGTGATAAGTGTTTGGATGACTGGAAGGAAGCAGCTCGACGACTAGAGGCTTCTCAACCTGCCAGCCTTGAAGATGGGAAAGTTGTGTTCAATTGTATAAAATTAAGCTATGATTACTTGATATCTGATGATGCCAGGTCATGCTTCTTGCTTTGTTGCCTCTTCCCAGAAGACTATGATATCCAAATTGAAGAGTTGTTAAAGTATGGGATGGGGAAAGGGTTGTTTCAAGATTCCTGCACAATGCAAGATGCCAGAGTAACTGCACATTTGGTGGTCAAGTACCTTAAAGATTGTAGCTTGCTTTTGGAAACTGAACAGGACGGATGTGTAAAGATGCATGATGTCATTCGGGATGTGGCCATAAGGATTTCGTTGTCTGAGGATGGCCCACTGTTTTTTGTACAAGCTGGTTGTCAATTAAAGGAATGGCCAAATATGGGCGCACAAGAAGGCTACTCTGCTATTTCACTAATTATGAATAAAATTTGCAAGCTTCCTGAAGAGTTGGTATGTCCGAAACTCCGTATTTTGTTTATGCAAAGTAATGTTTGGTTGAATGAGATCCCAGAATATTTCTTTCAAAGTCTGAATGTGTTGAGGGTATTAGATCTAAGCCGAACCAGAATTTCTTTTCTACCCACATCATTCAATATCTTGATCAACCTCCAGACCTTGTATCTAGATGGTTGCTTGTCAACCATAGATAACATATCCATACTCGGAAAATTGAAAAAACTTGAGATTCTTAGTATGAGAGAACTTCCTCTTGTGGAACTGCCAAAAGAAATCGGAGATTTGAGCCATCTAAGGATGTTAGACATCACTGGTGATTATGTTGATAATGTTCCGTCTGAAGTGATATGCAACCTTAATTTGTTAGAGGAACTATACATGCAGTGTAATTTTGCAGACTGGGGGAGTAAAGTTGAGGGAGCAGGAGAAGAAAATATTGTTGCGTTTGATGAGTTAACTAGCTTGTCATGTTTAAACATTTTGAAGGTTTTCATATCTGATGCTAAATGTTTGCCTAAAGATGTGGAGGTTGAACCAAATTGGGTAAACTTTGATATTTGTATCAGCAGAGACCCATCCATCAGAAAAGTGTCGTTGGTTTCAAGTTCTTCACCCGCTTATACAAGAGCTTTGACTCTTGGTACAACCATCAATGCCTTACCAGGTTGGTTTATCAATGCGGTGACAATGAAAGCAGAGAAACTACAGTACATAATGTGTTGGGGATTAGATAACATTCTTGTAGAATATGACTGTGGGAGATTACATGCACTGAAGTATTTATCTGTTATTGGCCCCAATGAGAACTTGAAAATGTTGATGAACACAATAACACGCTTTTGGAATGAACCAGTGTTTCAGAACTTGGAAGAGTTGCATCTCGTTCAAGTGGATAGCCTGAAGGAGTTATGTGTTGGAGAATTACCACTTGGGTCTCTATGCAATCTTAGGTTATTGAAGGTTAAACATTGTTATGAATTGGTGGATGCACTTCTATCGTCACAATTGCTGCAGAGATTACAAAATCTGGAAACACTGATTTGTGAAGAGATAGATGAATTGGAATATGTTTTTGGATGTGAAGGGATTAAGCCAGAACAAATGATATTGACACAATTGATAGAAATGAGATTGGAGAATCTAGGGAAACTAATAAAGATATGGAATGGTCCTGCTCCTTGTGCAATCTTCCAGAATCTTCAGAGTTTGGTAGTTTCCAGGTGCTTTGAATTGGAAAATCTCTTCACAGCTGATGTAGCTCAATGTCTTTTGCACTTGGAAGATCTTTCAGTGGAGCTTTGCCCAATGTTGGACAACATTATTGAAGCAAGTAAGGACACAGTGGACAACAAGATTGTTTTTCCAGAATTGAAGAACTTAACTTTGATAAAGCTTCCACAACTCACAAGGTTCTGCAGCAGTACAGGCAGTGCTATTGAGTGCCCTTTACTGGAATACTTGTATGTGGAGAGGTGCCCCCAGTTTTCAACGTCTGACTTCCATAGCAGGAACAAAGTCGAACCCAAACATCCACAACATTTGGACATTGTATTGAGAAGGGCCAGAGCAGAGAAAAGAAGAAAAATCAAATTCACAGAGGTCGTCCAAGAAGACAGTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

2101

Amino Acids

237.88

Weight (kDa)

5.57

Isoelectric Point (pI)

46.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 26 - 80 2e-13 TIR domain
TIR PF01582 83 - 242 1.1e-44 TIR domain
TIR_2 PF13676 85 - 169 8e-15 TIR domain
NB-ARC PF00931 263 - 426 7.8e-26 NB-ARC domain
LRR_8 PF13855 632 - 691 2.4e-07 Leucine rich repeat
LRR_14 PF23598 694 - 758 5.2e-07 Leucine-rich repeat region
LRR_RPS2 PF23247 923 - 1065 1.1e-20 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1022 - 1126 1.8e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
NB-ARC PF00931 1192 - 1355 8.5e-27 NB-ARC domain
LRR_8 PF13855 1562 - 1620 9.3e-07 Leucine rich repeat
LRR_14 PF23598 1615 - 1691 1.1e-09 Leucine-rich repeat region
LRR_RPS2 PF23247 1850 - 1995 4.7e-27 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013889)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19200 AT4G34419
fragaria_vesca FvH4_2g06210
malus_domestica MD05G1079600.v1.1 MD10G1093900.v1.1
prunus_persica Prupe.8G123500_v2.0.a1
pyrus_communis pycom10g07730
rosa_chinensis RchiOBHm_Chr6g0256981
rosa_laevigata RLG00000014667
rosa_multiflora Rmu_sc0000659.1_g000036
rosa_roxburghii Rroxscaffold_7G00208390
rosa_rugosa Rorug05G0575900
rosa_samantha Rh6AG094500 Rh6BG085800 Rh6CG081900 Rh6DG077600
rosa_wichuraiana Rw6G008090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 495, 1079
Acc36I ACCTGC 3 cut(s) 2395, 3046, 4234
Acc65I GGTACC 1 cut(s) 1662
AccB1I GGYRCC 2 cut(s) 1662, 6156
AccI GTMKAC 5 cut(s) 846, 1345, 3186, 3445, 3633
AciI CCGC 3 cut(s) 3488, 5298, 5363
AclWI GGATC 5 cut(s) 382, 2523, 3853, 3866, 4726
AcoI YGGCCR 4 cut(s) 621, 1811, 4527, 4598
AcsI RAATTY 8 cut(s) 477, 915, 2220, 3756, 3912, 4653, 4794, 6272
AcvI CACGTG 2 cut(s) 2971, 3074
AdeI CACNNNGTG 2 cut(s) 685, 4169
AfiI CCNNNNNNNGG 7 cut(s) 961, 2882, 3202, 3376, 4607, 5992, 6133
AflIII ACRYGT 6 cut(s) 84, 886, 2357, 2968, 2970, 3673
AhlI ACTAGT 1 cut(s) 1393
AjiI CACGTC 1 cut(s) 85
AjnI CCWGG 7 cut(s) 5, 1083, 1945, 2830, 4301, 5344, 5900
AloI GAACNNNNNNTCC 2 cut(s) 3482, 3514
Alw21I GWGCWC 3 cut(s) 898, 1423, 3823
Alw44I GTGCAC 1 cut(s) 894
AlwI GGATC 5 cut(s) 382, 2523, 3853, 3866, 4726
AlwNI CAGNNNCTG 2 cut(s) 65, 2077
Ama87I CYCGRG 1 cut(s) 1416
ApaLI GTGCAC 1 cut(s) 894
ApoI RAATTY 8 cut(s) 477, 915, 2220, 3756, 3912, 4653, 4794, 6272
AseI ATTAAT 1 cut(s) 1850
Asp700I GAANNNNTTC 2 cut(s) 969, 5171
Asp718I GGTACC 1 cut(s) 1662
AspLEI GCGC 2 cut(s) 814, 4613
AsuC2I CCSGG 1 cut(s) 1878
AvaI CYCGRG 1 cut(s) 1416
AvaII GGWCC 7 cut(s) 589, 1257, 2696, 2863, 3062, 4044, 5852
BaeGI GKGCMC 5 cut(s) 898, 1378, 3368, 6128, 6161
BalI TGGCCA 3 cut(s) 1813, 4529, 4600
BamHI GGATCC 1 cut(s) 3858
BanI GGYRCC 2 cut(s) 1662, 6156
BanII GRGCYC 1 cut(s) 3823
BarI GAAGNNNNNNTAC 8 cut(s) 109, 141, 228, 244, 260, 276, 2594, 2626
BbrPI CACGTG 2 cut(s) 2971, 3074
BbsI GAAGAC 7 cut(s) 1022, 1557, 1770, 3180, 4124, 4344, 6300
Bbv12I GWGCWC 3 cut(s) 898, 1423, 3823
BceAI ACGGC 6 cut(s) 484, 821, 865, 1290, 1640, 3393
BcgI CGANNNNNNTGC 4 cut(s) 815, 849, 5661, 5695
BciT130I CCWGG 7 cut(s) 7, 1085, 1947, 2832, 4303, 5346, 5902
BciVI GTATCC 2 cut(s) 955, 3742
BclI TGATCA 4 cut(s) 181, 316, 2175, 4827
BcnI CCSGG 1 cut(s) 1878
BcuI ACTAGT 1 cut(s) 1393
BfmI CTRYAG 7 cut(s) 149, 284, 1916, 2944, 5387, 5693, 6097
BfoI RGCGCY 1 cut(s) 815
BfuAI ACCTGC 3 cut(s) 2395, 3046, 4234
BfuI GTATCC 2 cut(s) 955, 3742
BglI GCCNNNNNGGC 1 cut(s) 2001
BglII AGATCT 6 cut(s) 187, 322, 667, 1071, 4777, 5965
Bme1390I CCNGG 8 cut(s) 7, 1085, 1878, 1947, 2832, 4303, 5346, 5902
Bme18I GGWCC 7 cut(s) 589, 1257, 2696, 2863, 3062, 4044, 5852
BmeT110I CYCGRG 1 cut(s) 1416
BmgBI CACGTC 1 cut(s) 85
BmiI GGNNCC 6 cut(s) 1664, 2698, 2742, 3860, 5479, 6158
BmrFI CCNGG 8 cut(s) 7, 1085, 1878, 1947, 2832, 4303, 5346, 5902
BmrI ACTGGG 4 cut(s) 2694, 3364, 5092, 6157
BmuI ACTGGG 4 cut(s) 2694, 3364, 5092, 6157
BoxI GACNNNNGTC 1 cut(s) 507
BpiI GAAGAC 7 cut(s) 1022, 1557, 1770, 3180, 4124, 4344, 6300
BpmI CTGGAG 3 cut(s) 1886, 2951, 4820
Bpu10I CCTNAGC 1 cut(s) 1991
BpuEI CTTGAG 6 cut(s) 2135, 2432, 2554, 3138, 3769, 4928
BpuMI CCSGG 1 cut(s) 1878
Bsa29I ATCGAT 1 cut(s) 1295
BsaAI YACGTR 3 cut(s) 2614, 2971, 3074
BsaBI GATNNNNATC 4 cut(s) 186, 321, 577, 666
BsaI GGTCTC 3 cut(s) 2841, 5247, 5622
BsaJI CCNNGG 8 cut(s) 792, 876, 2656, 2831, 3339, 3540, 3663, 3990
BsaXI ACNNNNNCTCC 2 cut(s) 1872, 1902
Bsc4I CCNNNNNNNGG 7 cut(s) 961, 2882, 3202, 3376, 4607, 5992, 6133
Bse118I RCCGGY 1 cut(s) 2000
Bse3DI GCAATG 3 cut(s) 3084, 3112, 3483
Bse8I GATNNNNATC 4 cut(s) 186, 321, 577, 666
BseBI CCWGG 7 cut(s) 7, 1085, 1947, 2832, 4303, 5346, 5902
BseCI ATCGAT 1 cut(s) 1295
BseDI CCNNGG 8 cut(s) 792, 876, 2656, 2831, 3339, 3540, 3663, 3990
BseJI GATNNNNATC 4 cut(s) 186, 321, 577, 666
BseLI CCNNNNNNNGG 7 cut(s) 961, 2882, 3202, 3376, 4607, 5992, 6133
BseMI GCAATG 3 cut(s) 3084, 3112, 3483
BseMII CTCAG 3 cut(s) 3002, 3516, 4539
BseRI GAGGAG 6 cut(s) 169, 304, 1064, 3660, 3812, 3815
BseSI GKGCMC 5 cut(s) 898, 1378, 3368, 6128, 6161
BseYI CCCAGC 3 cut(s) 632, 994, 3917
BsgI GTGCAG 4 cut(s) 718, 3702, 4390, 4416
Bsh1285I CGRYCG 2 cut(s) 280, 1299
BshNI GGYRCC 2 cut(s) 1662, 6156
BshVI ATCGAT 1 cut(s) 1295
BsiEI CGRYCG 2 cut(s) 280, 1299
BsiHKAI GWGCWC 3 cut(s) 898, 1423, 3823
BsiHKCI CYCGRG 1 cut(s) 1416
BsiSI CCGG 2 cut(s) 1878, 2001
BslFI GGGAC 3 cut(s) 385, 2471, 2682
BslI CCNNNNNNNGG 7 cut(s) 961, 2882, 3202, 3376, 4607, 5992, 6133
BsmFI GGGAC 3 cut(s) 385, 2471, 2682
BsmI GAATGC 4 cut(s) 1466, 1930, 2731, 4001
Bso31I GGTCTC 3 cut(s) 2841, 5247, 5622
BsoBI CYCGRG 1 cut(s) 1416
Bsp1286I GDGCHC 7 cut(s) 898, 1378, 1423, 3368, 3823, 6128, 6161
Bsp1407I TGTACA 3 cut(s) 2265, 3300, 4570
Bsp19I CCATGG 6 cut(s) 792, 876, 2656, 3540, 3663, 3990
BspACI CCGC 3 cut(s) 3488, 5298, 5363
BspCNI CTCAG 3 cut(s) 3003, 3517, 4540
BspDI ATCGAT 1 cut(s) 1295
BspHI TCATGA 1 cut(s) 1198
BspLI GGNNCC 6 cut(s) 1664, 2698, 2742, 3860, 5479, 6158
BspMAI CTGCAG 4 cut(s) 153, 288, 5697, 6101
BspMI ACCTGC 3 cut(s) 2395, 3046, 4234
BspPI GGATC 5 cut(s) 382, 2523, 3853, 3866, 4726
BspQI GCTCTTC 3 cut(s) 2400, 2709, 3811
BspT107I GGYRCC 2 cut(s) 1662, 6156
BspTNI GGTCTC 3 cut(s) 2841, 5247, 5622
BsrDI GCAATG 3 cut(s) 3084, 3112, 3483
BsrFI RCCGGY 1 cut(s) 2000
BsrGI TGTACA 3 cut(s) 2265, 3300, 4570
BssAI RCCGGY 1 cut(s) 2000
BssECI CCNNGG 8 cut(s) 792, 876, 2656, 2831, 3339, 3540, 3663, 3990
BssT1I CCWWGG 7 cut(s) 792, 876, 2656, 3339, 3540, 3663, 3990
Bst2UI CCWGG 7 cut(s) 7, 1085, 1947, 2832, 4303, 5346, 5902
BstAUI TGTACA 3 cut(s) 2265, 3300, 4570
BstBAI YACGTR 3 cut(s) 2614, 2971, 3074
BstC8I GCNNGC 7 cut(s) 859, 1682, 1792, 1811, 4231, 4469, 4661
BstDSI CCRYGG 6 cut(s) 792, 876, 2656, 3540, 3663, 3990
BstH2I RGCGCY 1 cut(s) 815
BstHHI GCGC 2 cut(s) 814, 4613
BstMCI CGRYCG 2 cut(s) 280, 1299
BstNI CCWGG 7 cut(s) 7, 1085, 1947, 2832, 4303, 5346, 5902
BstNSI RCATGY 8 cut(s) 890, 902, 2272, 2361, 3677, 4125, 5065, 5453
BstPAI GACNNNNGTC 1 cut(s) 507
BstSCI CCNGG 8 cut(s) 5, 1083, 1876, 1945, 2830, 4301, 5344, 5900
BstSFI CTRYAG 7 cut(s) 149, 284, 1916, 2944, 5387, 5693, 6097
BstSLI GKGCMC 5 cut(s) 898, 1378, 3368, 6128, 6161
BstSNI TACGTA 1 cut(s) 2614
BstV2I GAAGAC 7 cut(s) 1022, 1557, 1770, 3180, 4124, 4344, 6300
BstX2I RGATCY 9 cut(s) 187, 322, 667, 1071, 2528, 3858, 4731, 4777, 5965
BstYI RGATCY 9 cut(s) 187, 322, 667, 1071, 2528, 3858, 4731, 4777, 5965
Bsu15I ATCGAT 1 cut(s) 1295
BsuI GTATCC 2 cut(s) 955, 3742
BsuTUI ATCGAT 1 cut(s) 1295
BtgI CCRYGG 6 cut(s) 792, 876, 2656, 3540, 3663, 3990
BtrI CACGTC 1 cut(s) 85
BtsI GCAGTG 4 cut(s) 602, 831, 5072, 6118
BveI ACCTGC 3 cut(s) 2395, 3046, 4234
Cac8I GCNNGC 7 cut(s) 859, 1682, 1792, 1811, 4231, 4469, 4661
CaiI CAGNNNCTG 2 cut(s) 65, 2077
CciI TCATGA 1 cut(s) 1198
CfoI GCGC 2 cut(s) 814, 4613
Cfr10I RCCGGY 1 cut(s) 2000
ClaI ATCGAT 1 cut(s) 1295
CsiI ACCWGGT 1 cut(s) 5344
CspCI CAANNNNNGTGG 2 cut(s) 6204, 6239
DraI TTTAAA 3 cut(s) 1803, 1935, 5157
DraIII CACNNNGTG 2 cut(s) 685, 4169
EaeI YGGCCR 4 cut(s) 621, 1811, 4527, 4598
Ecl136II GAGCTC 1 cut(s) 3821
Eco105I TACGTA 1 cut(s) 2614
Eco130I CCWWGG 7 cut(s) 792, 876, 2656, 3339, 3540, 3663, 3990
Eco24I GRGCYC 1 cut(s) 3823
Eco31I GGTCTC 3 cut(s) 2841, 5247, 5622
Eco32I GATATC 5 cut(s) 1864, 2238, 3325, 4290, 4348
Eco47I GGWCC 7 cut(s) 589, 1257, 2696, 2863, 3062, 4044, 5852
Eco53kI GAGCTC 1 cut(s) 3821
Eco72I CACGTG 2 cut(s) 2971, 3074
Eco88I CYCGRG 1 cut(s) 1416
EcoICRI GAGCTC 1 cut(s) 3821
EcoRI GAATTC 2 cut(s) 477, 3912
EcoRII CCWGG 7 cut(s) 5, 1083, 1945, 2830, 4301, 5344, 5900
EcoRV GATATC 5 cut(s) 1864, 2238, 3325, 4290, 4348
EcoT14I CCWWGG 7 cut(s) 792, 876, 2656, 3339, 3540, 3663, 3990
EcoT22I ATGCAT 3 cut(s) 1722, 2399, 4509
EcoT38I GRGCYC 1 cut(s) 3823
ErhI CCWWGG 7 cut(s) 792, 876, 2656, 3339, 3540, 3663, 3990
FalI AAGNNNNNCTT 4 cut(s) 1508, 1540, 4057, 4089
FaqI GGGAC 3 cut(s) 385, 2471, 2682
FauI CCCGC 1 cut(s) 5305
FauNDI CATATG 2 cut(s) 2670, 3053
FbaI TGATCA 4 cut(s) 181, 316, 2175, 4827
FblI GTMKAC 5 cut(s) 846, 1345, 3186, 3445, 3633
FriOI GRGCYC 1 cut(s) 3823
GlaI GCGC 2 cut(s) 813, 4612
GsaI CCCAGC 3 cut(s) 636, 998, 3921
GsuI CTGGAG 3 cut(s) 1886, 2951, 4820
HaeII RGCGCY 1 cut(s) 815
HapII CCGG 2 cut(s) 1878, 2001
HhaI GCGC 2 cut(s) 814, 4613
Hin6I GCGC 2 cut(s) 812, 4611
HinP1I GCGC 2 cut(s) 812, 4611
HincII GTYRAC 4 cut(s) 502, 3421, 4866, 5139
HindII GTYRAC 4 cut(s) 502, 3421, 4866, 5139
HindIII AAGCTT 4 cut(s) 2049, 2111, 4661, 6074
HpaI GTTAAC 1 cut(s) 5139
HpaII CCGG 2 cut(s) 1878, 2001
Hpy99I CGWCG 1 cut(s) 4209
HpyCH4IV ACGT 7 cut(s) 84, 801, 2613, 2970, 3073, 4070, 6173
HpySE526I ACGT 7 cut(s) 84, 801, 2613, 2970, 3073, 4070, 6173
HspAI GCGC 2 cut(s) 812, 4611
KpnI GGTACC 1 cut(s) 1666
Ksp22I TGATCA 4 cut(s) 181, 316, 2175, 4827
KspAI GTTAAC 1 cut(s) 5139
LguI GCTCTTC 3 cut(s) 2400, 2709, 3811
LmnI GCTCC 5 cut(s) 1141, 3073, 5102, 5863, 5977
MabI ACCWGGT 1 cut(s) 5344
MaeII ACGT 7 cut(s) 84, 801, 2613, 2970, 3073, 4070, 6173
MfeI CAATTG 8 cut(s) 231, 366, 387, 2425, 3167, 4258, 5687, 5798
MflI RGATCY 9 cut(s) 187, 322, 667, 1071, 2528, 3858, 4731, 4777, 5965
MhlI GDGCHC 7 cut(s) 898, 1378, 1423, 3368, 3823, 6128, 6161
MlsI TGGCCA 3 cut(s) 1813, 4529, 4600
MluNI TGGCCA 3 cut(s) 1813, 4529, 4600
MlyI GAGTC 3 cut(s) 1280, 2545, 5311
MmeI TCCRAC 5 cut(s) 844, 2797, 3631, 5578, 5973
Mox20I TGGCCA 3 cut(s) 1813, 4529, 4600
Mph1103I ATGCAT 3 cut(s) 1722, 2399, 4509
MroXI GAANNNNTTC 2 cut(s) 969, 5171
MscI TGGCCA 3 cut(s) 1813, 4529, 4600
MslI CAYNNNNRTG 5 cut(s) 891, 1471, 3351, 3678, 5655
Msp20I TGGCCA 3 cut(s) 1813, 4529, 4600
MspA1I CMGCKG 6 cut(s) 47, 65, 148, 1646, 3725, 5933
MspI CCGG 2 cut(s) 1878, 2001
MspR9I CCNGG 8 cut(s) 7, 1085, 1878, 1947, 2832, 4303, 5346, 5902
MssI GTTTAAAC 1 cut(s) 5157
MunI CAATTG 8 cut(s) 231, 366, 387, 2425, 3167, 4258, 5687, 5798
Mva1269I GAATGC 4 cut(s) 1466, 1930, 2731, 4001
MvaI CCWGG 7 cut(s) 7, 1085, 1947, 2832, 4303, 5346, 5902
NciI CCSGG 1 cut(s) 1878
NcoI CCATGG 6 cut(s) 792, 876, 2656, 3540, 3663, 3990
NdeI CATATG 2 cut(s) 2670, 3053
NlaIV GGNNCC 6 cut(s) 1664, 2698, 2742, 3860, 5479, 6158
NmeAIII GCCGAG 1 cut(s) 1642
NmuCI GTSAC 5 cut(s) 829, 2791, 3616, 5365, 5682
NsiI ATGCAT 3 cut(s) 1722, 2399, 4509
NspI RCATGY 8 cut(s) 890, 902, 2272, 2361, 3677, 4125, 5065, 5453
PaeR7I CTCGAG 1 cut(s) 1416
PagI TCATGA 1 cut(s) 1198
PciI ACATGT 3 cut(s) 886, 2357, 3673
PciSI GCTCTTC 3 cut(s) 2400, 2709, 3811
PctI GAATGC 4 cut(s) 1466, 1930, 2731, 4001
PdmI GAANNNNTTC 2 cut(s) 969, 5171
PflFI GACNNNGTC 1 cut(s) 827
PfoI TCCNGGA 1 cut(s) 1083
Ple19I CGATCG 2 cut(s) 280, 1299
PleI GAGTC 3 cut(s) 1280, 2544, 5311
PmaCI CACGTG 2 cut(s) 2971, 3074
PmeI GTTTAAAC 1 cut(s) 5157
PmlI CACGTG 2 cut(s) 2971, 3074
PpsI GAGTC 3 cut(s) 1280, 2544, 5311
Ppu21I YACGTR 3 cut(s) 2614, 2971, 3074
PscI ACATGT 3 cut(s) 886, 2357, 3673
PshAI GACNNNNGTC 1 cut(s) 507
PshBI ATTAAT 1 cut(s) 1850
PsiI TTATAA 2 cut(s) 495, 1079
Psp124BI GAGCTC 1 cut(s) 3823
Psp6I CCWGG 7 cut(s) 5, 1083, 1945, 2830, 4301, 5344, 5900
PspCI CACGTG 2 cut(s) 2971, 3074
PspFI CCCAGC 3 cut(s) 632, 994, 3917
PspGI CCWGG 7 cut(s) 5, 1083, 1945, 2830, 4301, 5344, 5900
PspN4I GGNNCC 6 cut(s) 1664, 2698, 2742, 3860, 5479, 6158
PspXI VCTCGAGB 1 cut(s) 1416
PstI CTGCAG 4 cut(s) 153, 288, 5697, 6101
PstNI CAGNNNCTG 2 cut(s) 65, 2077
PsuI RGATCY 9 cut(s) 187, 322, 667, 1071, 2528, 3858, 4731, 4777, 5965
PsyI GACNNNGTC 1 cut(s) 827
PvuI CGATCG 2 cut(s) 280, 1299
PvuII CAGCTG 6 cut(s) 47, 65, 148, 1646, 3725, 5933
RseI CAYNNNNRTG 5 cut(s) 891, 1471, 3351, 3678, 5655
SacI GAGCTC 1 cut(s) 3823
SapI GCTCTTC 3 cut(s) 2400, 2709, 3811
SchI GAGTC 3 cut(s) 1280, 2545, 5311
ScrFI CCNGG 8 cut(s) 7, 1085, 1878, 1947, 2832, 4303, 5346, 5902
SduI GDGCHC 7 cut(s) 898, 1378, 1423, 3368, 3823, 6128, 6161
SexAI ACCWGGT 1 cut(s) 5344
SfcI CTRYAG 7 cut(s) 149, 284, 1916, 2944, 5387, 5693, 6097
Sfr274I CTCGAG 1 cut(s) 1416
SinI GGWCC 7 cut(s) 589, 1257, 2696, 2863, 3062, 4044, 5852
SlaI CTCGAG 1 cut(s) 1416
SmiI ATTTAAAT 1 cut(s) 1935
SmiMI CAYNNNNRTG 5 cut(s) 891, 1471, 3351, 3678, 5655
SmlI CTYRAG 7 cut(s) 1416, 2114, 2411, 2533, 3153, 3748, 4907
SmoI CTYRAG 7 cut(s) 1416, 2114, 2411, 2533, 3153, 3748, 4907
SnaBI TACGTA 1 cut(s) 2614
SpeI ACTAGT 1 cut(s) 1393
SsiI CCGC 3 cut(s) 3488, 5298, 5363
SspI AATATT 5 cut(s) 1750, 2368, 2890, 4742, 5119
SstI GAGCTC 1 cut(s) 3823
StyD4I CCNGG 8 cut(s) 5, 1083, 1876, 1945, 2830, 4301, 5344, 5900
StyI CCWWGG 7 cut(s) 792, 876, 2656, 3339, 3540, 3663, 3990
SwaI ATTTAAAT 1 cut(s) 1935
TaiI ACGT 7 cut(s) 87, 804, 2616, 2973, 3076, 4073, 6176
TatI WGTACW 8 cut(s) 706, 733, 2265, 3300, 3503, 4570, 5391, 6104
TseFI GTSAC 5 cut(s) 829, 2791, 3616, 5365, 5682
Tsp45I GTSAC 5 cut(s) 829, 2791, 3616, 5365, 5682
TspGWI ACGGA 6 cut(s) 1195, 3242, 3651, 4506, 4682, 5007
Tth111I GACNNNGTC 1 cut(s) 827
VneI GTGCAC 1 cut(s) 894
VpaK11BI GGWCC 7 cut(s) 589, 1257, 2696, 2863, 3062, 4044, 5852
VspI ATTAAT 1 cut(s) 1850
XapI RAATTY 8 cut(s) 477, 915, 2220, 3756, 3912, 4653, 4794, 6272
XbaI TCTAGA 3 cut(s) 2059, 2957, 4849
XceI RCATGY 8 cut(s) 890, 902, 2272, 2361, 3677, 4125, 5065, 5453
XcmI CCANNNNNNNNNTGG 1 cut(s) 2707
XhoI CTCGAG 1 cut(s) 1416
XmiI GTMKAC 5 cut(s) 846, 1345, 3186, 3445, 3633
XmnI GAANNNNTTC 2 cut(s) 969, 5171
Zsp2I ATGCAT 3 cut(s) 1722, 2399, 4509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.