Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0008625

Physical Location & Seq

Genomic Coordinates Forward (+)

29955974 .. 29968845

Gene Structure 12872 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1201400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1671 bp

ATGCAAGCCCCAGTGCTCGTCCTCAAAGATTCGTTGAAGCGTGAGTCAGGGAGTAAAGTACACCATGGAAATATCCAGGCATCGAAGGCTGTTGCTGACATAATCCGGACAACCTTGGGCCCTCGGTCCATGCTCAAGATGCTACTTGACGCAAGTGGAGGAATTGTAGTGACTAACGACGGAAATGCCATTCTGCGTGAATTGGATCTTGCTCACCCTGCAGCAAAGTCAATGATTGAATTAAGTCGCACACAAGATGAAGAAGTAGGCGATGGAACAACATCTGTCATTGTCCTTGCTGGTGAGATGCTCCATGTTGCAGAAGCATTTATTGACAAGCGCTATCATCCTACTGTTATTTGCCGAGCCTACAACAAAGCTCTGGAGGATGCTATTGCTGTTCTTGACAAAATAGCAATGGACATTGATGTGAAGGATCGTGCAACAATGTTGGGGCTGGTCAAGAGTTGTATCGGTACAAAGTTCACTAGTCAGTTTGGGGATTTAATTGCTGATTTAGCACTTGATGCCACCACAATAGTCGGGGTGGACCTTGGCCAGGGTCTACGAGAAGTGGATATCAAAAAGTACATTAAGGTTGAGAAGGTTCCTGGTGGCCAGTTGGAAGATTCAGTGGTTCTGAAAGGAGTAATGTTTAACAAAGATGTTATTGCACCTGGAAAAATGAGAAGAAAGATTGTCAACCCAAGGATCATTCTTCTTGACTGTCCTCTTGAGTATAAGAAAGGGGAGAACCAAACAAATGCTGAGTTACTTAAAGAAGAAGATTGGGGAGTCCTACTAAAACTGGAAGAAGAATACATCGAGAGCCTGTGCGTGCAGATATTGAAGTTTAAGCCAGATGTGGTTATCACAGAGAAGGGGCTTAGTGACTTGGCATGCCATTACCTGAGCAAGGCTGGCGTCAGTGCAATCAGGAGGTTGCGGAAAACAGATAATAACCGAATTGCTAAGGCCTGTGGAGCTGTTATTGTTAACAGACCAGATGAATTGCAAGAGTCTGATGTTGGTACAGGGGCTGGGCTATTTGAGGTCAAGAAAATTGGTGATGAGTATTTTGCTTTCATTGTTGATTGCAAAGATCCCAAAGCCTGTACTATACTCTTAAGAGGCCCTAGTAAGGATCTCTTAAATGAAGTGGAAAGAAATTTGCAGGATGCTATGTCAGTAGCAAGAAACATCCTCAAAAATCCAAAACTTGTTCCTGGTGGTGGTGCTACAGAGTTAACTGTATCTGCAACATTAAAGCAAAAGAGCTCATCTGTAGAAGGTATCGAAAAGTGGCCATATGAAGCTGCCGCTATAGCTTTTGAGGCTATACCACGAACTTTGGCTCAGAATTGCGGGGTTAATGTGATTAGAACAATGACGGCACTGCAAGGAAAGCATGCAAATGGTGAAAATGCATGGATTGGCATAGACGGAAACACTGGTGCGATAACTGACGTGAAGGAGAAAAAGATATGGGATGCATACAACGTCAAGGCTCAGACCTTTAAAACAGCCATAGAATCCGCTTGCCTACTTCTCAGAATCGATGACATTGTGAGTGGGATTAAGAAGAAGCAGCCTCCTGGTTCCAAGGGTCCTTCAAAGCCTCAAGTTGAGACAGAAGGCGACGCTGATAATGAGCAAATGATTCCCGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005832 GO:0005975 GO:0006457 GO:0006458 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006884 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0008361 GO:0009311 GO:0009653 GO:0009719 GO:0009725 GO:0009751 GO:0009826 GO:0009987 GO:0009992 GO:0010033 GO:0014070 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019725 GO:0023052 GO:0030104 GO:0032502 GO:0032535 GO:0032989 GO:0032991 GO:0036211 GO:0040007 GO:0042221 GO:0042493 GO:0042592 GO:0043170 GO:0043412 GO:0044183 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0046677 GO:0048589 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051082 GO:0051716 GO:0055082 GO:0060560 GO:0061077 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0090066 GO:0101031 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cpn60_TCP1

Protein Analysis

557

Amino Acids

60.46

Weight (kDa)

6.12

Isoelectric Point (pI)

34.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cpn60_TCP1	PF00118	31 - 524	1.3e-157	TCP-1/cpn60 chaperonin family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008625)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G26360
fragaria_vesca	FvH4_3g15890 FvH4_3g15890
malus_domestica	MD05G1219100.v1.1 MD10G1201400.v1.1
prunus_persica	Prupe.2G068500_v2.0.a1 Prupe.2G068500_v2.0.a1 Prupe.4G141000_v2.0.a1 Prupe.6G064200_v2.0.a1
pyrus_communis	pycom05g20030 pycom10g17240
rosa_chinensis	RchiOBHm_Chr5g0026711
rosa_laevigata	RLG00000032949
rosa_multiflora	Rmu_sc0027336.1_g000001
rosa_roxburghii	Rroxscaffold_1G00052760
rosa_rugosa	Rorug05G0095000
rosa_samantha	Rh4CG005800 Rh5BG185000 Rh5CG204800 Rh5DG187200 Rh5DG243000
rosa_wichuraiana	Rw1G016630 Rw5G017080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	565
AccIII	TCCGGA	1 cut(s)	105
AciI	CCGC	4 cut(s)	946, 1320, 1365, 1536
AclWI	GGATC	5 cut(s)	213, 444, 719, 1097, 1152
AcoI	YGGCCR	3 cut(s)	556, 616, 1304
AcsI	RAATTY	1 cut(s)	1168
AcyI	GRCGYC	1 cut(s)	924
AfaI	GTAC	5 cut(s)	60, 478, 590, 1033, 1117
AfeI	AGCGCT	1 cut(s)	341
AfiI	CCNNNNNNNGG	4 cut(s)	559, 916, 1141, 1232
AflII	CTTAAG	1 cut(s)	1126
AgsI	TTSAA	4 cut(s)	37, 239, 850, 1614
AhlI	ACTAGT	1 cut(s)	488
AjiI	CACGTC	1 cut(s)	1468
AjnI	CCWGG	6 cut(s)	75, 558, 610, 676, 1225, 1594
AluBI	AGCT	5 cut(s)	380, 986, 1278, 1316, 1328
AluI	AGCT	5 cut(s)	380, 986, 1278, 1316, 1328
Alw21I	GWGCWC	2 cut(s)	18, 1280
Alw26I	GTCTC	1 cut(s)	1622
AlwI	GGATC	5 cut(s)	213, 444, 719, 1097, 1152
AlwNI	CAGNNNCTG	1 cut(s)	1040
Ama87I	CYCGRG	1 cut(s)	1664
Aor13HI	TCCGGA	1 cut(s)	105
Aor51HI	AGCGCT	1 cut(s)	341
AoxI	GGCC	6 cut(s)	118, 556, 616, 975, 1132, 1304
ApaI	GGGCCC	1 cut(s)	122
ApeKI	GCWGC	3 cut(s)	221, 1316, 1588
ApoI	RAATTY	1 cut(s)	1168
AspLEI	GCGC	1 cut(s)	342
AspS9I	GGNCC	6 cut(s)	118, 119, 126, 550, 1133, 1607
AsuHPI	GGTGA	4 cut(s)	206, 314, 1079, 1430
AvaI	CYCGRG	1 cut(s)	1664
AvaII	GGWCC	3 cut(s)	126, 550, 1607
BaeGI	GKGCMC	1 cut(s)	122
BalI	TGGCCA	3 cut(s)	558, 618, 1306
BanII	GRGCYC	2 cut(s)	122, 1280
Bbv12I	GWGCWC	2 cut(s)	18, 1280
BbvI	GCAGC	3 cut(s)	233, 1303, 1600
BccI	CCATC	1 cut(s)	266
BceAI	ACGGC	1 cut(s)	1407
BcgI	CGANNNNNNTGC	2 cut(s)	167, 201
BciT130I	CCWGG	6 cut(s)	77, 560, 612, 678, 1227, 1596
BcoDI	GTCTC	1 cut(s)	1622
BcuI	ACTAGT	1 cut(s)	488
BfaI	CTAG	2 cut(s)	489, 1137
BfmI	CTRYAG	4 cut(s)	219, 1239, 1284, 1323
BfoI	RGCGCY	1 cut(s)	343
BfrI	CTTAAG	1 cut(s)	1126
BisI	GCNGC	4 cut(s)	222, 1317, 1320, 1589
BlsI	GCNGC	4 cut(s)	223, 1318, 1321, 1590
Bme1390I	CCNGG	6 cut(s)	77, 560, 612, 678, 1227, 1596
Bme18I	GGWCC	3 cut(s)	126, 550, 1607
BmeT110I	CYCGRG	1 cut(s)	1664
BmgBI	CACGTC	1 cut(s)	1468
BmgT120I	GGNCC	6 cut(s)	118, 119, 126, 550, 1133, 1607
BmiI	GGNNCC	4 cut(s)	120, 609, 1600, 1608
BmrFI	CCNGG	6 cut(s)	77, 560, 612, 678, 1227, 1596
BmrI	ACTGGG	1 cut(s)	5
BmsI	GCATC	7 cut(s)	89, 129, 297, 379, 517, 1168, 1480
BmuI	ACTGGG	1 cut(s)	5
BpmI	CTGGAG	1 cut(s)	404
Bpu10I	CCTNAGC	2 cut(s)	911, 972
BpuEI	CTTGAG	3 cut(s)	119, 755, 1605
Bsa29I	ATCGAT	1 cut(s)	1557
BsaHI	GRCGYC	1 cut(s)	924
BsaJI	CCNNGG	7 cut(s)	64, 114, 122, 553, 559, 707, 1602
BsaWI	WCCGGW	1 cut(s)	105
BsaXI	ACNNNNNCTCC	2 cut(s)	150, 180
Bsc4I	CCNNNNNNNGG	4 cut(s)	559, 916, 1141, 1232
Bse1I	ACTGG	4 cut(s)	11, 619, 813, 1456
Bse3DI	GCAATG	1 cut(s)	423
BseAI	TCCGGA	1 cut(s)	105
BseBI	CCWGG	6 cut(s)	77, 560, 612, 678, 1227, 1596
BseCI	ATCGAT	1 cut(s)	1557
BseDI	CCNNGG	7 cut(s)	64, 114, 122, 553, 559, 707, 1602
BseGI	GGATG	5 cut(s)	346, 394, 1183, 1200, 1495
BseLI	CCNNNNNNNGG	4 cut(s)	559, 916, 1141, 1232
BseMI	GCAATG	1 cut(s)	423
BseMII	CTCAG	5 cut(s)	759, 902, 1370, 1523, 1564
BseNI	ACTGG	4 cut(s)	11, 619, 813, 1456
BseSI	GKGCMC	1 cut(s)	122
BseXI	GCAGC	3 cut(s)	233, 1303, 1600
BseYI	CCCAGC	1 cut(s)	1040
BsgI	GTGCAG	1 cut(s)	860
BshFI	GGCC	6 cut(s)	120, 558, 618, 977, 1134, 1306
BshVI	ATCGAT	1 cut(s)	1557
BsiHKAI	GWGCWC	2 cut(s)	18, 1280
BsiHKCI	CYCGRG	1 cut(s)	1664
BsiSI	CCGG	1 cut(s)	106
BslI	CCNNNNNNNGG	4 cut(s)	559, 916, 1141, 1232
BsmAI	GTCTC	1 cut(s)	1622
BsnI	GGCC	6 cut(s)	120, 558, 618, 977, 1134, 1306
BsoBI	CYCGRG	1 cut(s)	1664
Bsp120I	GGGCCC	1 cut(s)	118
Bsp1286I	GDGCHC	3 cut(s)	18, 122, 1280
Bsp13I	TCCGGA	1 cut(s)	105
Bsp143I	GATC	5 cut(s)	205, 436, 711, 1102, 1144
Bsp19I	CCATGG	1 cut(s)	64
BspACI	CCGC	4 cut(s)	946, 1320, 1365, 1536
BspANI	GGCC	6 cut(s)	120, 558, 618, 977, 1134, 1306
BspCNI	CTCAG	5 cut(s)	760, 903, 1369, 1522, 1563
BspDI	ATCGAT	1 cut(s)	1557
BspEI	TCCGGA	1 cut(s)	105
BspLI	GGNNCC	4 cut(s)	120, 609, 1600, 1608
BspMAI	CTGCAG	1 cut(s)	223
BspPI	GGATC	5 cut(s)	213, 444, 719, 1097, 1152
BspTI	CTTAAG	1 cut(s)	1126
BsrDI	GCAATG	1 cut(s)	423
BsrI	ACTGG	4 cut(s)	11, 619, 813, 1456
BssECI	CCNNGG	7 cut(s)	64, 114, 122, 553, 559, 707, 1602
BssMI	GATC	5 cut(s)	205, 436, 711, 1102, 1144
BssNI	GRCGYC	1 cut(s)	924
BssT1I	CCWWGG	5 cut(s)	64, 114, 553, 707, 1602
Bst2UI	CCWGG	6 cut(s)	77, 560, 612, 678, 1227, 1596
Bst4CI	ACNGT	3 cut(s)	355, 728, 1252
BstACI	GRCGYC	1 cut(s)	924
BstAFI	CTTAAG	1 cut(s)	1126
BstAPI	GCANNNNNTGC	1 cut(s)	527
BstC8I	GCNNGC	6 cut(s)	6, 839, 901, 922, 1410, 1540
BstDEI	CTNAG	7 cut(s)	768, 887, 911, 972, 1356, 1509, 1550
BstDSI	CCRYGG	1 cut(s)	64
BstENI	CCTNNNNNAGG	2 cut(s)	557, 914
BstF5I	GGATG	5 cut(s)	346, 394, 1183, 1200, 1495
BstH2I	RGCGCY	1 cut(s)	343
BstHHI	GCGC	1 cut(s)	342
BstKTI	GATC	5 cut(s)	208, 439, 714, 1105, 1147
BstMAI	GTCTC	1 cut(s)	1622
BstMBI	GATC	5 cut(s)	205, 436, 711, 1102, 1144
BstNI	CCWGG	6 cut(s)	77, 560, 612, 678, 1227, 1596
BstNSI	RCATGY	2 cut(s)	903, 1412
BstSCI	CCNGG	6 cut(s)	75, 558, 610, 676, 1225, 1594
BstSFI	CTRYAG	4 cut(s)	219, 1239, 1284, 1323
BstSLI	GKGCMC	1 cut(s)	122
BstV1I	GCAGC	3 cut(s)	233, 1303, 1600
BstX2I	RGATCY	3 cut(s)	205, 1102, 1144
BstYI	RGATCY	3 cut(s)	205, 1102, 1144
Bsu15I	ATCGAT	1 cut(s)	1557
BsuRI	GGCC	6 cut(s)	120, 558, 618, 977, 1134, 1306
BsuTUI	ATCGAT	1 cut(s)	1557
BtgI	CCRYGG	1 cut(s)	64
BtgZI	GCGATG	1 cut(s)	285
BtrI	CACGTC	1 cut(s)	1468
BtsCI	GGATG	5 cut(s)	346, 394, 1183, 1200, 1495
BtsI	GCAGTG	1 cut(s)	1394
BtsIMutI	CAGTG	5 cut(s)	18, 639, 934, 1394, 1449
Cac8I	GCNNGC	6 cut(s)	6, 839, 901, 922, 1410, 1540
CaiI	CAGNNNCTG	1 cut(s)	1040
CfoI	GCGC	1 cut(s)	342
Cfr13I	GGNCC	6 cut(s)	118, 119, 126, 550, 1133, 1607
ClaI	ATCGAT	1 cut(s)	1557
CseI	GACGC	3 cut(s)	158, 913, 1649
Csp6I	GTAC	5 cut(s)	59, 477, 589, 1032, 1116
CspCI	CAANNNNNGTGG	2 cut(s)	1332, 1367
CviAII	CATG	6 cut(s)	65, 130, 314, 900, 1409, 1428
CviQI	GTAC	5 cut(s)	59, 477, 589, 1032, 1116
DdeI	CTNAG	7 cut(s)	768, 887, 911, 972, 1356, 1509, 1550
DpnI	GATC	5 cut(s)	207, 438, 713, 1104, 1146
DpnII	GATC	5 cut(s)	205, 436, 711, 1102, 1144
DraI	TTTAAA	1 cut(s)	1519
EaeI	YGGCCR	3 cut(s)	556, 616, 1304
Ecl136II	GAGCTC	1 cut(s)	1278
Eco130I	CCWWGG	5 cut(s)	64, 114, 553, 707, 1602
Eco147I	AGGCCT	1 cut(s)	977
Eco24I	GRGCYC	2 cut(s)	122, 1280
Eco32I	GATATC	1 cut(s)	580
Eco47I	GGWCC	3 cut(s)	126, 550, 1607
Eco47III	AGCGCT	1 cut(s)	341
Eco53kI	GAGCTC	1 cut(s)	1278
Eco88I	CYCGRG	1 cut(s)	1664
EcoICRI	GAGCTC	1 cut(s)	1278
EcoNI	CCTNNNNNAGG	2 cut(s)	557, 914
EcoO109I	RGGNCCY	3 cut(s)	119, 1133, 1607
EcoRII	CCWGG	6 cut(s)	75, 558, 610, 676, 1225, 1594
EcoRV	GATATC	1 cut(s)	580
EcoT14I	CCWWGG	5 cut(s)	64, 114, 553, 707, 1602
EcoT22I	ATGCAT	2 cut(s)	1429, 1495
EcoT38I	GRGCYC	2 cut(s)	122, 1280
ErhI	CCWWGG	5 cut(s)	64, 114, 553, 707, 1602
FaeI	CATG	6 cut(s)	68, 133, 317, 903, 1412, 1431
FalI	AAGNNNNNCTT	2 cut(s)	1133, 1165
FatI	CATG	6 cut(s)	64, 129, 313, 899, 1408, 1427
FauI	CCCGC	1 cut(s)	1358
FauNDI	CATATG	1 cut(s)	1309
FblI	GTMKAC	1 cut(s)	565
Fnu4HI	GCNGC	4 cut(s)	222, 1317, 1320, 1589
FokI	GGATG	5 cut(s)	333, 401, 1187, 1190, 1502
FriOI	GRGCYC	2 cut(s)	122, 1280
Fsp4HI	GCNGC	4 cut(s)	222, 1317, 1320, 1589
FspBI	CTAG	2 cut(s)	489, 1137
GlaI	GCGC	1 cut(s)	341
GluI	GCNGC	4 cut(s)	222, 1317, 1320, 1589
GsaI	CCCAGC	1 cut(s)	1044
GsuI	CTGGAG	1 cut(s)	404
HaeII	RGCGCY	1 cut(s)	343
HaeIII	GGCC	6 cut(s)	120, 558, 618, 977, 1134, 1306
HapII	CCGG	1 cut(s)	106
HgaI	GACGC	3 cut(s)	158, 913, 1649
HhaI	GCGC	1 cut(s)	342
Hin1I	GRCGYC	1 cut(s)	924
Hin1II	CATG	6 cut(s)	68, 133, 317, 903, 1412, 1431
Hin6I	GCGC	1 cut(s)	340
HinP1I	GCGC	1 cut(s)	340
HincII	GTYRAC	3 cut(s)	703, 997, 1248
HindII	GTYRAC	3 cut(s)	703, 997, 1248
HinfI	GANTC	8 cut(s)	29, 44, 629, 795, 1019, 1532, 1554, 1660
HpaI	GTTAAC	2 cut(s)	997, 1248
HpaII	CCGG	1 cut(s)	106
HphI	GGTGA	4 cut(s)	206, 314, 1079, 1430
Hpy166II	GTNNAC	7 cut(s)	61, 486, 550, 566, 703, 997, 1248
Hpy188I	TCNGA	5 cut(s)	642, 1024, 1359, 1512, 1553
Hpy8I	GTNNAC	7 cut(s)	61, 486, 550, 566, 703, 997, 1248
Hpy99I	CGWCG	2 cut(s)	182, 1643
HpyAV	CCTTC	8 cut(s)	79, 427, 598, 874, 1283, 1465, 1620, 1628
HpyCH4III	ACNGT	3 cut(s)	355, 728, 1252
HpyCH4IV	ACGT	2 cut(s)	1467, 1500
HpyF3I	CTNAG	7 cut(s)	768, 887, 911, 972, 1356, 1509, 1550
HpySE526I	ACGT	2 cut(s)	1467, 1500
Hsp92I	GRCGYC	1 cut(s)	924
Hsp92II	CATG	6 cut(s)	68, 133, 317, 903, 1412, 1431
HspAI	GCGC	1 cut(s)	340
Kpn2I	TCCGGA	1 cut(s)	105
KspAI	GTTAAC	2 cut(s)	997, 1248
Kzo9I	GATC	5 cut(s)	205, 436, 711, 1102, 1144
LmnI	GCTCC	2 cut(s)	315, 983
Lsp1109I	GCAGC	3 cut(s)	233, 1303, 1600
LweI	GCATC	7 cut(s)	89, 129, 297, 379, 517, 1168, 1480
MaeI	CTAG	2 cut(s)	489, 1137
MaeII	ACGT	2 cut(s)	1467, 1500
MaeIII	GTNAC	3 cut(s)	169, 771, 890
MalI	GATC	5 cut(s)	207, 438, 713, 1104, 1146
MboI	GATC	5 cut(s)	205, 436, 711, 1102, 1144
MboII	GAAGA	9 cut(s)	272, 638, 702, 710, 794, 797, 824, 827, 1594
MflI	RGATCY	3 cut(s)	205, 1102, 1144
MhlI	GDGCHC	3 cut(s)	18, 122, 1280
MlsI	TGGCCA	3 cut(s)	558, 618, 1306
MluCI	AATT	9 cut(s)	162, 200, 239, 507, 966, 1010, 1062, 1168, 1360
MluNI	TGGCCA	3 cut(s)	558, 618, 1306
MlyI	GAGTC	3 cut(s)	53, 804, 1028
MmeI	TCCRAC	1 cut(s)	603
Mox20I	TGGCCA	3 cut(s)	558, 618, 1306
Mph1103I	ATGCAT	2 cut(s)	1429, 1495
MroI	TCCGGA	1 cut(s)	105
MscI	TGGCCA	3 cut(s)	558, 618, 1306
MslI	CAYNNNNRTG	2 cut(s)	428, 1413
Msp20I	TGGCCA	3 cut(s)	558, 618, 1306
MspCI	CTTAAG	1 cut(s)	1126
MspI	CCGG	1 cut(s)	106
MspR9I	CCNGG	6 cut(s)	77, 560, 612, 678, 1227, 1596
MvaI	CCWGG	6 cut(s)	77, 560, 612, 678, 1227, 1596
NcoI	CCATGG	1 cut(s)	64
NdeI	CATATG	1 cut(s)	1309
NdeII	GATC	5 cut(s)	205, 436, 711, 1102, 1144
NlaIII	CATG	6 cut(s)	68, 133, 317, 903, 1412, 1431
NlaIV	GGNNCC	4 cut(s)	120, 609, 1600, 1608
NmeAIII	GCCGAG	1 cut(s)	389
NmuCI	GTSAC	2 cut(s)	169, 890
NsiI	ATGCAT	2 cut(s)	1429, 1495
NspI	RCATGY	2 cut(s)	903, 1412
PaeI	GCATGC	2 cut(s)	903, 1412
PceI	AGGCCT	1 cut(s)	977
PfeI	GAWTC	5 cut(s)	29, 629, 1532, 1554, 1660
PkrI	GCNGC	4 cut(s)	223, 1318, 1321, 1590
PleI	GAGTC	3 cut(s)	52, 803, 1027
PpsI	GAGTC	3 cut(s)	52, 803, 1027
PpuMI	RGGWCCY	1 cut(s)	1607
Psp124BI	GAGCTC	1 cut(s)	1280
Psp5II	RGGWCCY	1 cut(s)	1607
Psp6I	CCWGG	6 cut(s)	75, 558, 610, 676, 1225, 1594
PspFI	CCCAGC	1 cut(s)	1040
PspGI	CCWGG	6 cut(s)	75, 558, 610, 676, 1225, 1594
PspN4I	GGNNCC	4 cut(s)	120, 609, 1600, 1608
PspOMI	GGGCCC	1 cut(s)	118
PspPI	GGNCC	6 cut(s)	118, 119, 126, 550, 1133, 1607
PspPPI	RGGWCCY	1 cut(s)	1607
PstI	CTGCAG	1 cut(s)	223
PstNI	CAGNNNCTG	1 cut(s)	1040
PsuI	RGATCY	3 cut(s)	205, 1102, 1144
RsaI	GTAC	5 cut(s)	60, 478, 590, 1033, 1117
RsaNI	GTAC	5 cut(s)	59, 477, 589, 1032, 1116
RseI	CAYNNNNRTG	2 cut(s)	428, 1413
SacI	GAGCTC	1 cut(s)	1280
SatI	GCNGC	4 cut(s)	222, 1317, 1320, 1589
Sau3AI	GATC	5 cut(s)	205, 436, 711, 1102, 1144
Sau96I	GGNCC	6 cut(s)	118, 119, 126, 550, 1133, 1607
SchI	GAGTC	3 cut(s)	53, 804, 1028
ScrFI	CCNGG	6 cut(s)	77, 560, 612, 678, 1227, 1596
SduI	GDGCHC	3 cut(s)	18, 122, 1280
SfaNI	GCATC	7 cut(s)	89, 129, 297, 379, 517, 1168, 1480
SfcI	CTRYAG	4 cut(s)	219, 1239, 1284, 1323
SinI	GGWCC	3 cut(s)	126, 550, 1607
SmiMI	CAYNNNNRTG	2 cut(s)	428, 1413
SmlI	CTYRAG	4 cut(s)	134, 734, 1126, 1620
SmoI	CTYRAG	4 cut(s)	134, 734, 1126, 1620
SpeI	ACTAGT	1 cut(s)	488
SphI	GCATGC	2 cut(s)	903, 1412
Sse9I	AATT	9 cut(s)	162, 200, 239, 507, 966, 1010, 1062, 1168, 1360
SseBI	AGGCCT	1 cut(s)	977
SsiI	CCGC	4 cut(s)	946, 1320, 1365, 1536
SspMI	CTAG	2 cut(s)	489, 1137
SstI	GAGCTC	1 cut(s)	1280
StuI	AGGCCT	1 cut(s)	977
StyD4I	CCNGG	6 cut(s)	75, 558, 610, 676, 1225, 1594
StyI	CCWWGG	5 cut(s)	64, 114, 553, 707, 1602
TaaI	ACNGT	3 cut(s)	355, 728, 1252
TaiI	ACGT	2 cut(s)	1470, 1503
TaqI	TCGA	4 cut(s)	83, 825, 1296, 1557
TaqII	GACCGA	1 cut(s)	114
TasI	AATT	9 cut(s)	162, 200, 239, 507, 966, 1010, 1062, 1168, 1360
TatI	WGTACW	3 cut(s)	58, 588, 1115
TauI	GCSGC	1 cut(s)	1322
TfiI	GAWTC	5 cut(s)	29, 629, 1532, 1554, 1660
TscAI	CASTG	5 cut(s)	18, 639, 934, 1401, 1456
TseFI	GTSAC	2 cut(s)	169, 890
TseI	GCWGC	3 cut(s)	221, 1316, 1588
Tsp45I	GTSAC	2 cut(s)	169, 890
TspDTI	ATGAA	5 cut(s)	273, 1023, 1075, 1170, 1326
TspGWI	ACGGA	2 cut(s)	195, 1458
TspRI	CASTG	5 cut(s)	18, 639, 934, 1401, 1456
Vha464I	CTTAAG	1 cut(s)	1126
VpaK11BI	GGWCC	3 cut(s)	126, 550, 1607
XagI	CCTNNNNNAGG	2 cut(s)	557, 914
XapI	RAATTY	1 cut(s)	1168
XceI	RCATGY	2 cut(s)	903, 1412
XmiI	GTMKAC	1 cut(s)	565
XspI	CTAG	2 cut(s)	489, 1137
Zsp2I	ATGCAT	2 cut(s)	1429, 1495

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.