Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0016786

Physical Location & Seq

Genomic Coordinates Forward (+)

32523021 .. 32523557

Gene Structure 537 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1228500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 537 bp

ATGGCTCAAACATCCCCTCCTCCAATTCCTTCAGTGCCTCCAATTCCCAGTCCCATCTCAATAAGAACACTTTGTGATCAAAACCCTAATCATGATCAATTCCAACCCATTCATCATCTCAATTTTATGGTCATCTTGGCAGCAATTTTGTGTGCCCTTGTGTGTGTTCTTGGTATCAATTCCATGTTGCACTGCGTTTTTCAATGCGCAAGTCGTGCCGTCATTGAACCGGTCGAATGGGTAGCTTCACGCCGGCTTAATTCAGGCCTAAAGAAGAAGGAAATGGTGGCTTTGCCTACTTCAACTTACGCTGATTCAGGTACTACTTCTCCATCTTCGTCGTCTTCAGGCTGCGCTATTTGTTTGGCTGATTTTTCTGATGGGGACAAGTTAAGGGTGCTGCCCAAGTGCAACCATCGGTTTCATGTTGCCTGCATTGACAAGTGGTTGCTCTGCCACTCTTCTTGTCCGACTTGCCGACATCGGCTCAAGTCCAGTGACTGCATGCCCTCTCTTTCTGAGATTGTCACAGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009555 GO:0009791 GO:0009889 GO:0009900 GO:0009901 GO:0009908 GO:0009987 GO:0010498 GO:0010565 GO:0010817 GO:0016567 GO:0016740 GO:0019216 GO:0019217 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0022414 GO:0030163 GO:0031323 GO:0031326 GO:0032350 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0036211 GO:0042304 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0046885 GO:0046890 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048608 GO:0048609 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0051603 GO:0061458 GO:0061630 GO:0061659 GO:0062012 GO:0065007 GO:0065008 GO:0070647 GO:0071704 GO:0080090 GO:0080140 GO:0080141 GO:0090567 GO:0099402 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

179

Amino Acids

19.34

Weight (kDa)

8.25

Isoelectric Point (pI)

60.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	118 - 160	7.2e-14	Ring finger domain
zf-RING_11	PF17123	118 - 146	9.8e-12	RING-like zinc finger
zf-C3HC4_2	PF13923	118 - 159	1.1e-06	Zinc finger, C3HC4 type (RING finger)
zf-rbx1	PF12678	131 - 160	9.9e-07	RING-H2 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016786)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g12790
malus_domestica	MD05G1247700.v1.1 MD10G1228500.v1.1
prunus_persica	Prupe.4G116300_v2.0.a1
pyrus_communis	pycom05g22380
rosa_chinensis	RchiOBHm_Chr5g0020761
rosa_laevigata	RLG00000032559
rosa_roxburghii	Rroxscaffold_1G00057540
rosa_rugosa	Rorug05G0062800
rosa_samantha	Rh5AG152600 Rh5BG152300 Rh5CG163800 Rh5DG151800
rosa_wichuraiana	Rw5G013530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	208
AcuI	CTGAAG	2 cut(s)	15, 330
AdeI	CACNNNGTG	1 cut(s)	74
AfaI	GTAC	1 cut(s)	322
AgeI	ACCGGT	1 cut(s)	229
AgsI	TTSAA	3 cut(s)	203, 227, 303
AluBI	AGCT	1 cut(s)	245
AluI	AGCT	1 cut(s)	245
AlwNI	CAGNNNCTG	1 cut(s)	501
AoxI	GGCC	1 cut(s)	265
ApeKI	GCWGC	3 cut(s)	140, 351, 400
AsiGI	ACCGGT	1 cut(s)	229
AspLEI	GCGC	2 cut(s)	209, 356
BaeGI	GKGCMC	1 cut(s)	157
BbsI	GAAGAC	1 cut(s)	336
BbvI	GCAGC	3 cut(s)	152, 338, 387
BccI	CCATC	4 cut(s)	62, 340, 374, 423
BceAI	ACGGC	1 cut(s)	203
BclI	TGATCA	2 cut(s)	76, 94
BisI	GCNGC	3 cut(s)	141, 352, 401
BlsI	GCNGC	3 cut(s)	142, 353, 402
BmrI	ACTGGG	1 cut(s)	42
BmuI	ACTGGG	1 cut(s)	42
BpiI	GAAGAC	1 cut(s)	336
BpuEI	CTTGAG	1 cut(s)	473
BsaWI	WCCGGW	1 cut(s)	229
BsaXI	ACNNNNNCTCC	3 cut(s)	31, 313, 343
Bse118I	RCCGGY	2 cut(s)	229, 252
Bse1I	ACTGG	2 cut(s)	48, 495
BseGI	GGATG	1 cut(s)	11
BseMII	CTCAG	1 cut(s)	510
BseNI	ACTGG	2 cut(s)	48, 495
BseRI	GAGGAG	1 cut(s)	9
BseSI	GKGCMC	1 cut(s)	157
BseXI	GCAGC	3 cut(s)	152, 338, 387
Bsh1285I	CGRYCG	1 cut(s)	234
BshFI	GGCC	1 cut(s)	267
BshTI	ACCGGT	1 cut(s)	229
BsiEI	CGRYCG	1 cut(s)	234
BsiSI	CCGG	2 cut(s)	230, 253
BslFI	GGGAC	2 cut(s)	36, 398
BsmFI	GGGAC	2 cut(s)	36, 398
BsnI	GGCC	1 cut(s)	267
Bsp1286I	GDGCHC	1 cut(s)	157
Bsp143I	GATC	2 cut(s)	76, 94
BspANI	GGCC	1 cut(s)	267
BspCNI	CTCAG	1 cut(s)	511
BspHI	TCATGA	1 cut(s)	91
BsrFI	RCCGGY	2 cut(s)	229, 252
BsrI	ACTGG	2 cut(s)	48, 495
BssAI	RCCGGY	2 cut(s)	229, 252
BssMI	GATC	2 cut(s)	76, 94
Bst6I	CTCTTC	1 cut(s)	466
BstAPI	GCANNNNNTGC	1 cut(s)	215
BstC8I	GCNNGC	3 cut(s)	254, 433, 506
BstDEI	CTNAG	1 cut(s)	519
BstF5I	GGATG	1 cut(s)	11
BstHHI	GCGC	2 cut(s)	209, 356
BstKTI	GATC	2 cut(s)	79, 97
BstMBI	GATC	2 cut(s)	76, 94
BstMCI	CGRYCG	1 cut(s)	234
BstMWI	GCNNNNNNNGC	1 cut(s)	215
BstNSI	RCATGY	1 cut(s)	508
BstSLI	GKGCMC	1 cut(s)	157
BstV1I	GCAGC	3 cut(s)	152, 338, 387
BstV2I	GAAGAC	1 cut(s)	336
BsuRI	GGCC	1 cut(s)	267
BtsCI	GGATG	1 cut(s)	11
BtsI	GCAGTG	1 cut(s)	190
BtsIMutI	CAGTG	3 cut(s)	39, 190, 502
Cac8I	GCNNGC	3 cut(s)	254, 433, 506
CaiI	CAGNNNCTG	1 cut(s)	501
CciI	TCATGA	1 cut(s)	91
CfoI	GCGC	2 cut(s)	209, 356
Cfr10I	RCCGGY	2 cut(s)	229, 252
Csp6I	GTAC	1 cut(s)	321
CspAI	ACCGGT	1 cut(s)	229
CspCI	CAANNNNNGTGG	2 cut(s)	446, 481
CviAII	CATG	4 cut(s)	92, 184, 425, 505
CviJI	RGCY	9 cut(s)	5, 245, 256, 267, 290, 351, 368, 487, 533
CviKI_1	RGCY	9 cut(s)	5, 245, 256, 267, 290, 351, 368, 487, 533
CviQI	GTAC	1 cut(s)	321
DdeI	CTNAG	1 cut(s)	519
DpnI	GATC	2 cut(s)	78, 96
DpnII	GATC	2 cut(s)	76, 94
DraIII	CACNNNGTG	1 cut(s)	74
Eam1104I	CTCTTC	1 cut(s)	466
EarI	CTCTTC	1 cut(s)	466
Eco147I	AGGCCT	1 cut(s)	267
Eco57I	CTGAAG	2 cut(s)	15, 330
FaeI	CATG	4 cut(s)	95, 187, 428, 508
FaiI	YATR	5 cut(s)	93, 128, 185, 426, 506
FaqI	GGGAC	2 cut(s)	36, 398
FatI	CATG	4 cut(s)	91, 183, 424, 504
FbaI	TGATCA	2 cut(s)	76, 94
Fnu4HI	GCNGC	3 cut(s)	141, 352, 401
Fsp4HI	GCNGC	3 cut(s)	141, 352, 401
FspI	TGCGCA	1 cut(s)	208
GlaI	GCGC	2 cut(s)	208, 355
GluI	GCNGC	3 cut(s)	141, 352, 401
HaeIII	GGCC	1 cut(s)	267
HapII	CCGG	2 cut(s)	230, 253
HhaI	GCGC	2 cut(s)	209, 356
Hin1II	CATG	4 cut(s)	95, 187, 428, 508
Hin6I	GCGC	2 cut(s)	207, 354
HinP1I	GCGC	2 cut(s)	207, 354
HinfI	GANTC	1 cut(s)	314
HpaII	CCGG	2 cut(s)	230, 253
Hpy188I	TCNGA	3 cut(s)	379, 471, 520
Hpy188III	TCNNGA	1 cut(s)	92
Hpy99I	CGWCG	1 cut(s)	343
HpyAV	CCTTC	2 cut(s)	39, 271
HpyCH4V	TGCA	4 cut(s)	190, 411, 435, 504
HpyF10VI	GCNNNNNNNGC	1 cut(s)	215
HpyF3I	CTNAG	1 cut(s)	519
Hsp92II	CATG	4 cut(s)	95, 187, 428, 508
HspAI	GCGC	2 cut(s)	207, 354
KroI	GCCGGC	1 cut(s)	252
KroNI	GCCGGC	1 cut(s)	254
Ksp22I	TGATCA	2 cut(s)	76, 94
Kzo9I	GATC	2 cut(s)	76, 94
LpnPI	CCDG	8 cut(s)	61, 243, 249, 266, 303, 333, 445, 508
Lsp1109I	GCAGC	3 cut(s)	152, 338, 387
MaeIII	GTNAC	2 cut(s)	497, 526
MalI	GATC	2 cut(s)	78, 96
MboI	GATC	2 cut(s)	76, 94
MboII	GAAGA	4 cut(s)	286, 327, 336, 453
MhlI	GDGCHC	1 cut(s)	157
MluCI	AATT	7 cut(s)	24, 42, 98, 121, 144, 178, 259
MmeI	TCCRAC	2 cut(s)	127, 494
MnlI	CCTC	4 cut(s)	27, 30, 48, 520
MroNI	GCCGGC	1 cut(s)	252
MseI	TTAA	2 cut(s)	258, 392
MspI	CCGG	2 cut(s)	230, 253
MwoI	GCNNNNNNNGC	1 cut(s)	215
NaeI	GCCGGC	1 cut(s)	254
NdeII	GATC	2 cut(s)	76, 94
NgoMIV	GCCGGC	1 cut(s)	252
NlaIII	CATG	4 cut(s)	95, 187, 428, 508
NmuCI	GTSAC	2 cut(s)	497, 526
NsbI	TGCGCA	1 cut(s)	208
NspI	RCATGY	1 cut(s)	508
PaeI	GCATGC	1 cut(s)	508
PagI	TCATGA	1 cut(s)	91
PceI	AGGCCT	1 cut(s)	267
PdiI	GCCGGC	1 cut(s)	254
PfeI	GAWTC	1 cut(s)	314
PinAI	ACCGGT	1 cut(s)	229
PkrI	GCNGC	3 cut(s)	142, 353, 402
PstNI	CAGNNNCTG	1 cut(s)	501
RsaI	GTAC	1 cut(s)	322
RsaNI	GTAC	1 cut(s)	321
SaqAI	TTAA	2 cut(s)	258, 392
SatI	GCNGC	3 cut(s)	141, 352, 401
Sau3AI	GATC	2 cut(s)	76, 94
SduI	GDGCHC	1 cut(s)	157
SetI	ASST	2 cut(s)	247, 322
SmlI	CTYRAG	1 cut(s)	488
SmoI	CTYRAG	1 cut(s)	488
SphI	GCATGC	1 cut(s)	508
Sse9I	AATT	7 cut(s)	24, 42, 98, 121, 144, 178, 259
SseBI	AGGCCT	1 cut(s)	267
StuI	AGGCCT	1 cut(s)	267
TaqI	TCGA	1 cut(s)	234
TasI	AATT	7 cut(s)	24, 42, 98, 121, 144, 178, 259
TfiI	GAWTC	1 cut(s)	314
Tru1I	TTAA	2 cut(s)	258, 392
Tru9I	TTAA	2 cut(s)	258, 392
TscAI	CASTG	3 cut(s)	39, 197, 502
TseFI	GTSAC	2 cut(s)	497, 526
TseI	GCWGC	3 cut(s)	140, 351, 400
Tsp45I	GTSAC	2 cut(s)	497, 526
TspDTI	ATGAA	2 cut(s)	101, 413
TspRI	CASTG	3 cut(s)	39, 197, 502
XceI	RCATGY	1 cut(s)	508

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.