MD10G1229600.v1.1

RIBOSOMAL protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011035
Physical Location & Seq
Forward (+)
32648063 .. 32649796
1734 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1229600.v1.1.491

Sequence Viewer

Length: 645 bp
ATGAGTTTGATCTTAAGATTACGGAATCATTTGCCTAATGGGTTCTGCAGAAGACCCCTTATTTCTTCCCTTGGAGCACTGAATTCTGGTAGATTAAATGGGTTTTGTCGAAACTTTGGTCAACCTGCAAGGCAAGAAGAGGAAGAGGAGGAAGAAGTGGAGATTGACCAGAGGAGACTCCCTGCTGATTATGATCCTGCTACATTTGATCCCACAGAGCATCGTAGTCCTCCAACTGATCGGGTTTTTAGGCTCGTAGATGAAATATCATCACTCACATTGGTTGAAGTTGCTGAGCTGGGTTCCATTTTGATGAGAAAAAAGGGAATGACGGAGCCGCCAGTTGTGGGAGTTATGAAGCCAGGAGCTGCTGGACTAGGATTGGCAATGAAGGGACCGTCAGCAGCTAAGGAGGAGAAGAAACCAGAAAAGACTGTATTTGAATTGAAATTGGAGTCATTTGAGGCAGCGTCTAAAATAAAACTGATCAAGGAGGTAAGGAGCTTCACCGACTTAGGACTCAAGGAAGCAAAGGATTTAGTGGAGAAGGCGCCAGCGGTGCTAAAGACAGGAGTGTCAAAGGAAGATGGAGAACAACTGGTAGAGAAGCTGAAAGCTCTTGGCGCAAAGGTTTCTCTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L12 Ribosomal_L12_N

Protein Analysis

215

Amino Acids

23.56

Weight (kDa)

6.26

Isoelectric Point (pI)

51.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L12 PF00542 147 - 214 2.4e-22 Ribosomal protein L7/L12 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011035)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G70190 AT1G70190
fragaria_vesca FvH4_3g12640
malus_domestica MD05G1249000.v1.1 MD10G1229600.v1.1
prunus_persica Prupe.4G115200_v2.0.a1
pyrus_communis pycom05g22590 pycom10g19310
rosa_chinensis RchiOBHm_Chr5g0020611
rosa_laevigata RLG00000014901 RLG00000032543
rosa_multiflora Rmu_sc0005607.1_g000013
rosa_roxburghii Rroxscaffold_1G00057680
rosa_rugosa Rorug05G0061700 Rorug05G0061800
rosa_samantha Rh5AG151600 Rh5BG151200 Rh5CG162600 Rh5DG150500
rosa_wichuraiana Rw5G013430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 574
Acc36I ACCTGC 1 cut(s) 133
AccB1I GGYRCC 1 cut(s) 550
AciI CCGC 2 cut(s) 338, 557
AclWI GGATC 2 cut(s) 188, 203
AcsI RAATTY 1 cut(s) 82
AcyI GRCGYC 1 cut(s) 551
AfiI CCNNNNNNNGG 1 cut(s) 347
AflII CTTAAG 1 cut(s) 13
AgsI TTSAA 4 cut(s) 287, 443, 448, 641
AjnI CCWGG 1 cut(s) 361
AluBI AGCT 6 cut(s) 298, 368, 407, 504, 610, 617
AluI AGCT 6 cut(s) 298, 368, 407, 504, 610, 617
Alw21I GWGCWC 1 cut(s) 79
Alw26I GTCTC 1 cut(s) 169
AlwI GGATC 2 cut(s) 188, 203
AlwNI CAGNNNCTG 1 cut(s) 368
ApeKI GCWGC 3 cut(s) 368, 404, 467
ApoI RAATTY 1 cut(s) 82
ArsI GACNNNNNNTTYG 2 cut(s) 103, 135
AspLEI GCGC 2 cut(s) 553, 626
AspS9I GGNCC 1 cut(s) 395
AsuHPI GGTGA 1 cut(s) 499
AvaII GGWCC 1 cut(s) 395
BanI GGYRCC 1 cut(s) 550
BbsI GAAGAC 1 cut(s) 58
Bbv12I GWGCWC 1 cut(s) 79
BbvI GCAGC 3 cut(s) 355, 416, 479
BccI CCATC 1 cut(s) 581
BciT130I CCWGG 1 cut(s) 363
BclI TGATCA 1 cut(s) 486
BcoDI GTCTC 1 cut(s) 169
BfaI CTAG 1 cut(s) 377
BfmI CTRYAG 1 cut(s) 46
BfoI RGCGCY 1 cut(s) 554
BfrI CTTAAG 1 cut(s) 13
BfuAI ACCTGC 1 cut(s) 133
BisI GCNGC 4 cut(s) 338, 369, 405, 468
BlpI GCTNAGC 1 cut(s) 294
BlsI GCNGC 4 cut(s) 339, 370, 406, 469
Bme1390I CCNGG 1 cut(s) 363
Bme18I GGWCC 1 cut(s) 395
BmgT120I GGNCC 1 cut(s) 395
BmiI GGNNCC 4 cut(s) 304, 336, 396, 552
BmrFI CCNGG 1 cut(s) 363
BmsI GCATC 1 cut(s) 229
BpiI GAAGAC 1 cut(s) 58
Bpu10I CCTNAGC 1 cut(s) 408
Bpu1102I GCTNAGC 1 cut(s) 294
BpuEI CTTGAG 1 cut(s) 506
BsaBI GATNNNNATC 1 cut(s) 192
BsaHI GRCGYC 1 cut(s) 551
BsaJI CCNNGG 1 cut(s) 70
Bsc4I CCNNNNNNNGG 1 cut(s) 347
Bse1I ACTGG 2 cut(s) 341, 603
Bse3DI GCAATG 1 cut(s) 393
Bse8I GATNNNNATC 1 cut(s) 192
BseBI CCWGG 1 cut(s) 363
BseDI CCNNGG 1 cut(s) 70
BseJI GATNNNNATC 1 cut(s) 192
BseLI CCNNNNNNNGG 1 cut(s) 347
BseMI GCAATG 1 cut(s) 393
BseMII CTCAG 1 cut(s) 285
BseNI ACTGG 2 cut(s) 341, 603
BseRI GAGGAG 3 cut(s) 161, 187, 428
BseXI GCAGC 3 cut(s) 355, 416, 479
BseYI CCCAGC 1 cut(s) 298
BshNI GGYRCC 1 cut(s) 550
BsiHKAI GWGCWC 1 cut(s) 79
BslFI GGGAC 1 cut(s) 408
BslI CCNNNNNNNGG 1 cut(s) 347
BsmAI GTCTC 1 cut(s) 169
BsmFI GGGAC 1 cut(s) 408
Bsp1286I GDGCHC 1 cut(s) 79
Bsp143I GATC 5 cut(s) 9, 193, 208, 238, 486
Bsp1720I GCTNAGC 1 cut(s) 294
BspACI CCGC 2 cut(s) 338, 557
BspCNI CTCAG 1 cut(s) 286
BspLI GGNNCC 4 cut(s) 304, 336, 396, 552
BspMAI CTGCAG 1 cut(s) 50
BspMI ACCTGC 1 cut(s) 133
BspPI GGATC 2 cut(s) 188, 203
BspT107I GGYRCC 1 cut(s) 550
BspTI CTTAAG 1 cut(s) 13
BsrDI GCAATG 1 cut(s) 393
BsrI ACTGG 2 cut(s) 341, 603
BssECI CCNNGG 1 cut(s) 70
BssMI GATC 5 cut(s) 9, 193, 208, 238, 486
BssNI GRCGYC 1 cut(s) 551
BssT1I CCWWGG 1 cut(s) 70
Bst2UI CCWGG 1 cut(s) 363
Bst4CI ACNGT 2 cut(s) 399, 436
Bst6I CTCTTC 2 cut(s) 132, 138
BstACI GRCGYC 1 cut(s) 551
BstAFI CTTAAG 1 cut(s) 13
BstC8I GCNNGC 1 cut(s) 555
BstDEI CTNAG 3 cut(s) 294, 408, 514
BstH2I RGCGCY 1 cut(s) 554
BstHHI GCGC 2 cut(s) 553, 626
BstKTI GATC 5 cut(s) 12, 196, 211, 241, 489
BstMAI GTCTC 1 cut(s) 169
BstMBI GATC 5 cut(s) 9, 193, 208, 238, 486
BstMWI GCNNNNNNNGC 2 cut(s) 559, 623
BstNI CCWGG 1 cut(s) 363
BstSCI CCNGG 1 cut(s) 361
BstSFI CTRYAG 1 cut(s) 46
BstV1I GCAGC 3 cut(s) 355, 416, 479
BstV2I GAAGAC 1 cut(s) 58
BtsIMutI CAGTG 1 cut(s) 77
BveI ACCTGC 1 cut(s) 133
Cac8I GCNNGC 1 cut(s) 555
CaiI CAGNNNCTG 1 cut(s) 368
CfoI GCGC 2 cut(s) 553, 626
Cfr13I GGNCC 1 cut(s) 395
CseI GACGC 1 cut(s) 459
CviJI RGCY 9 cut(s) 253, 298, 337, 361, 368, 407, 504, 610, 617
CviKI_1 RGCY 9 cut(s) 253, 298, 337, 361, 368, 407, 504, 610, 617
DdeI CTNAG 3 cut(s) 294, 408, 514
DinI GGCGCC 1 cut(s) 552
DpnI GATC 5 cut(s) 11, 195, 210, 240, 488
DpnII GATC 5 cut(s) 9, 193, 208, 238, 486
DrdI GACNNNNNNGTC 1 cut(s) 574
DseDI GACNNNNNNGTC 1 cut(s) 574
Eam1104I CTCTTC 2 cut(s) 132, 138
EarI CTCTTC 2 cut(s) 132, 138
Eco130I CCWWGG 1 cut(s) 70
Eco47I GGWCC 1 cut(s) 395
EcoRI GAATTC 1 cut(s) 82
EcoRII CCWGG 1 cut(s) 361
EcoT14I CCWWGG 1 cut(s) 70
EgeI GGCGCC 1 cut(s) 552
EheI GGCGCC 1 cut(s) 552
ErhI CCWWGG 1 cut(s) 70
FaiI YATR 2 cut(s) 192, 356
FaqI GGGAC 1 cut(s) 408
FbaI TGATCA 1 cut(s) 486
Fnu4HI GCNGC 4 cut(s) 338, 369, 405, 468
Fsp4HI GCNGC 4 cut(s) 338, 369, 405, 468
FspBI CTAG 1 cut(s) 377
GlaI GCGC 2 cut(s) 552, 625
GluI GCNGC 4 cut(s) 338, 369, 405, 468
GsaI CCCAGC 1 cut(s) 302
HaeII RGCGCY 1 cut(s) 554
HgaI GACGC 1 cut(s) 459
HhaI GCGC 2 cut(s) 553, 626
Hin1I GRCGYC 1 cut(s) 551
Hin6I GCGC 2 cut(s) 551, 624
HinP1I GCGC 2 cut(s) 551, 624
HincII GTYRAC 1 cut(s) 122
HindII GTYRAC 1 cut(s) 122
HinfI GANTC 4 cut(s) 25, 177, 455, 519
HphI GGTGA 1 cut(s) 499
Hpy166II GTNNAC 1 cut(s) 122
Hpy188III TCNNGA 1 cut(s) 638
Hpy8I GTNNAC 1 cut(s) 122
HpyAV CCTTC 2 cut(s) 385, 541
HpyCH4III ACNGT 2 cut(s) 399, 436
HpyCH4V TGCA 2 cut(s) 48, 128
HpyF10VI GCNNNNNNNGC 2 cut(s) 559, 623
HpyF3I CTNAG 3 cut(s) 294, 408, 514
Hsp92I GRCGYC 1 cut(s) 551
HspAI GCGC 2 cut(s) 551, 624
KasI GGCGCC 1 cut(s) 550
Ksp22I TGATCA 1 cut(s) 486
Kzo9I GATC 5 cut(s) 9, 193, 208, 238, 486
LmnI GCTCC 4 cut(s) 74, 334, 365, 501
Lsp1109I GCAGC 3 cut(s) 355, 416, 479
LweI GCATC 1 cut(s) 229
MaeI CTAG 1 cut(s) 377
MalI GATC 5 cut(s) 11, 195, 210, 240, 488
MboI GATC 5 cut(s) 9, 193, 208, 238, 486
MboII GAAGA 7 cut(s) 57, 63, 149, 155, 164, 430, 596
MhlI GDGCHC 1 cut(s) 79
MluCI AATT 3 cut(s) 82, 443, 449
Mly113I GGCGCC 1 cut(s) 551
MlyI GAGTC 3 cut(s) 171, 464, 513
MmeI TCCRAC 1 cut(s) 257
MnlI CCTC 8 cut(s) 133, 139, 142, 165, 240, 406, 457, 487
MseI TTAA 2 cut(s) 14, 95
MslI CAYNNNNRTG 1 cut(s) 311
MspA1I CMGCKG 1 cut(s) 557
MspCI CTTAAG 1 cut(s) 13
MspR9I CCNGG 1 cut(s) 363
MvaI CCWGG 1 cut(s) 363
MwoI GCNNNNNNNGC 2 cut(s) 559, 623
NarI GGCGCC 1 cut(s) 551
NdeII GATC 5 cut(s) 9, 193, 208, 238, 486
NlaIV GGNNCC 4 cut(s) 304, 336, 396, 552
PfeI GAWTC 1 cut(s) 25
PkrI GCNGC 4 cut(s) 339, 370, 406, 469
PleI GAGTC 3 cut(s) 171, 463, 513
PluTI GGCGCC 1 cut(s) 554
PpsI GAGTC 3 cut(s) 171, 463, 513
Psp6I CCWGG 1 cut(s) 361
PspFI CCCAGC 1 cut(s) 298
PspGI CCWGG 1 cut(s) 361
PspN4I GGNNCC 4 cut(s) 304, 336, 396, 552
PspPI GGNCC 1 cut(s) 395
PstI CTGCAG 1 cut(s) 50
PstNI CAGNNNCTG 1 cut(s) 368
RseI CAYNNNNRTG 1 cut(s) 311
SaqAI TTAA 2 cut(s) 14, 95
SatI GCNGC 4 cut(s) 338, 369, 405, 468
Sau3AI GATC 5 cut(s) 9, 193, 208, 238, 486
Sau96I GGNCC 1 cut(s) 395
SchI GAGTC 3 cut(s) 171, 464, 513
ScrFI CCNGG 1 cut(s) 363
SduI GDGCHC 1 cut(s) 79
SetI ASST 9 cut(s) 127, 300, 370, 409, 498, 506, 612, 619, 633
SfaNI GCATC 1 cut(s) 229
SfcI CTRYAG 1 cut(s) 46
SfoI GGCGCC 1 cut(s) 552
SinI GGWCC 1 cut(s) 395
SmiMI CAYNNNNRTG 1 cut(s) 311
SmlI CTYRAG 2 cut(s) 13, 521
SmoI CTYRAG 2 cut(s) 13, 521
Sse9I AATT 3 cut(s) 82, 443, 449
SsiI CCGC 2 cut(s) 338, 557
SspDI GGCGCC 1 cut(s) 550
SspMI CTAG 1 cut(s) 377
StyD4I CCNGG 1 cut(s) 361
StyI CCWWGG 1 cut(s) 70
TaaI ACNGT 2 cut(s) 399, 436
TaqI TCGA 1 cut(s) 109
TasI AATT 3 cut(s) 82, 443, 449
TauI GCSGC 1 cut(s) 340
TfiI GAWTC 1 cut(s) 25
Tru1I TTAA 2 cut(s) 14, 95
Tru9I TTAA 2 cut(s) 14, 95
TscAI CASTG 1 cut(s) 84
TseI GCWGC 3 cut(s) 368, 404, 467
TspDTI ATGAA 3 cut(s) 276, 371, 404
TspGWI ACGGA 2 cut(s) 37, 347
TspRI CASTG 1 cut(s) 84
Vha464I CTTAAG 1 cut(s) 13
VpaK11BI GGWCC 1 cut(s) 395
XapI RAATTY 1 cut(s) 82
XspI CTAG 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.