Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0003112

Physical Location & Seq

Genomic Coordinates Reverse (-)

33680536 .. 33686016

Gene Structure 5481 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1241500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1257 bp

ATGCTTAATTTTGGCAGGGCCAGAAGTCAGTCAAGAACTAACAGAGCCATGTCTTTAGGAGGCATGGAGTACCCAGATCCCAAGAGGAAGAACAGCTTTGTTGGAAAAATTCTTTTGGCTGCTACTCTTACAGCATTATGTATTATTATGCTGAAACAATCCCCATCTTTTAATACCACAAGCTCGTTCTCCCTTCATGAAGCAGGGGTAACTCATGTCCTAGTAACTGGAGGTGCTGGATATATTGGTTCACATGCTGCATTACGACTCCTAAGGGATCAATATCGTGTAACTATTGTGGATAACCTCTCACGGGGAAACCTAGGTGCCGTCAAGGTTCTCCAAGAGTTATTTCCTGAACCTGGGAGGCTTCAGTTTATTTATGCTGACTTGGGGGATCCAAAATCCGTAAACAAAATATTTTCAGAGAATGCATTTGATGCTGTGATGCATTTTGCAGCTGTTGCATATGTTGGGGAAAGCACACTTGATCCACTTAAGTATTACCACAACATTACGTCAAATACCTTAGTGGTATTGGAGGCAATGGCTGCACATGGTGTGAAGACTTTGATATATTCCAGTACATGTGCAACATATGGTGAACCTGAGAAGATGCCTATTACTGAAGAAACTCCACAGCTACCAATTAATCCATATGGAAAAGCAAAGAAGATGGCAGAAGATATCATCTTGGATTTTCATAAGAATTCGGACATGGCAGTCATGATCCTGAGATACTTCAATGTCATTGGGTCGGATCCAGAGGGCAGATTGGGTGAAGCTCCCAGACCTGAACTTCGTGAACATGGACGAATATCAGGTGCTTGTTTTGATGCAGCTCGTGGTATCATTCCTGGGCTAAAGGTTCGAGGTACTGATTACAAAACATCTGATGGAACTTGCATAAGAGATTATATAGATGTCACTGATCTGGTTGATGCTCACGTTAAAGCTCTTGAGCATGCACAGCCCCGTAAAGTGGGAATCTACAATGTTGGAACTGGAAAAGGTAGTTCAGTTAAGGAGTTCGTGGAGGCGTGTAAGAAGGCAACTGGGGTGGACCTAAAAGTTGATTTCCTTCCCCGCAGGCCTGGTGACTACGCTGAAGTCTTCAGTGATCCATCTAAGATCCTACGTGAACTCAACTGGAGCGCTCAACACACCAACCTTCAAGAGAGTCTGCAGGTTGCGTGGAGATGGCAAAAAGCACATCGCGATGGGTACGGAACCAACCCGTTGGTGTTGTCTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005975 GO:0005996 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009790 GO:0009791 GO:0009793 GO:0009832 GO:0009856 GO:0009987 GO:0010154 GO:0012505 GO:0016051 GO:0016853 GO:0016854 GO:0016857 GO:0019321 GO:0019322 GO:0019566 GO:0019567 GO:0022414 GO:0032501 GO:0032502 GO:0042546 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044238 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0044706 GO:0046364 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0048868 GO:0050373 GO:0051704 GO:0061458 GO:0071554 GO:0071669 GO:0071704 GO:0071840 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

GDP_Man_Dehyd

Protein Analysis

419

Amino Acids

46.02

Weight (kDa)

8.61

Isoelectric Point (pI)

35.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RmlD_sub_bind	PF04321	72 - 252	6.7e-12	RmlD substrate binding domain
Epimerase	PF01370	73 - 334	1.8e-52	NAD dependent epimerase/dehydratase family
Polysacc_synt_2	PF02719	73 - 253	1.2e-12	Polysaccharide biosynthesis protein
GDP_Man_Dehyd	PF16363	74 - 388	5.6e-53	GDP-mannose 4,6 dehydratase
3Beta_HSD	PF01073	74 - 235	5.8e-14	3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4	PF07993	75 - 257	2.2e-06	Male sterility protein
NAD_binding_10	PF13460	77 - 250	4.2e-08	NAD(P)H-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003112)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G20460 AT5G44480 AT5G44480 AT5G44480 AT5G44480
fragaria_vesca	FvH4_3g11380 FvH4_3g11380 FvH4_3g11380 FvH4_3g11380 FvH4_3g11380 FvH4_3g11380
malus_domestica	MD05G1263800.v1.1 MD10G1241500.v1.1
prunus_persica	Prupe.4G101700_v2.0.a1 Prupe.4G101700_v2.0.a1 Prupe.4G101700_v2.0.a1 Prupe.4G101700_v2.0.a1 Prupe.4G101700_v2.0.a1 Prupe.4G101700_v2.0.a1 Prupe.4G101700_v2.0.a1 Prupe.4G101700_v2.0.a1 Prupe.4G101700_v2.0.a1
pyrus_communis	pycom05g24780 pycom10g20250
rosa_chinensis	RchiOBHm_Chr5g0018331
rosa_laevigata	RLG00000032352
rosa_multiflora	Rmu_sc0011919.1_g000008
rosa_roxburghii	Rroxscaffold_1G00059410
rosa_rugosa	Rorug05G0041300 Rorug05G0041300 Rorug05G0041400 Rorug05G0041500
rosa_samantha	Rh5AG134800 Rh5BG133400 Rh5CG145200 Rh5DG134800
rosa_wichuraiana	Rw5G011770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	722
Acc36I	ACCTGC	1 cut(s)	1177
AccB1I	GGYRCC	1 cut(s)	326
AccII	CGCG	1 cut(s)	1218
AciI	CCGC	1 cut(s)	1087
AcsI	RAATTY	2 cut(s)	108, 709
AcuI	CTGAAG	4 cut(s)	356, 648, 1099, 1128
AdeI	CACNNNGTG	1 cut(s)	560
AfaI	GTAC	4 cut(s)	71, 586, 877, 1226
AfeI	AGCGCT	1 cut(s)	1156
AfiI	CCNNNNNNNGG	3 cut(s)	313, 362, 982
AflII	CTTAAG	1 cut(s)	497
AflIII	ACRYGT	1 cut(s)	587
AgsI	TTSAA	2 cut(s)	745, 1175
AjnI	CCWGG	3 cut(s)	361, 856, 1093
AjuI	GAANNNNNNNTTGG	2 cut(s)	336, 368
AluBI	AGCT	7 cut(s)	96, 183, 461, 643, 785, 842, 956
AluI	AGCT	7 cut(s)	96, 183, 461, 643, 785, 842, 956
Aor51HI	AGCGCT	1 cut(s)	1156
AoxI	GGCC	2 cut(s)	18, 1091
ApeKI	GCWGC	5 cut(s)	119, 257, 458, 551, 839
ApoI	RAATTY	2 cut(s)	108, 709
ArsI	GACNNNNNNTTYG	2 cut(s)	783, 815
AseI	ATTAAT	1 cut(s)	651
AspA2I	CCTAGG	1 cut(s)	322
AspLEI	GCGC	1 cut(s)	1157
AspS9I	GGNCC	2 cut(s)	18, 1063
AsuHPI	GGTGA	3 cut(s)	614, 791, 1109
AvaII	GGWCC	1 cut(s)	1063
AvrII	CCTAGG	1 cut(s)	322
AxyI	CCTNAGG	1 cut(s)	272
BamHI	GGATCC	2 cut(s)	397, 760
BanI	GGYRCC	1 cut(s)	326
BauI	CACGAG	1 cut(s)	843
BbsI	GAAGAC	3 cut(s)	572, 1105, 1242
BbvI	GCAGC	5 cut(s)	106, 244, 470, 538, 851
BccI	CCATC	6 cut(s)	172, 670, 890, 1132, 1194, 1214
BceAI	ACGGC	1 cut(s)	314
BciT130I	CCWGG	3 cut(s)	363, 858, 1095
BfaI	CTAG	2 cut(s)	221, 323
BfmI	CTRYAG	1 cut(s)	1184
BfoI	RGCGCY	1 cut(s)	1158
BfrI	CTTAAG	1 cut(s)	497
BfuAI	ACCTGC	1 cut(s)	1177
BisI	GCNGC	5 cut(s)	120, 258, 459, 552, 840
BlnI	CCTAGG	1 cut(s)	322
BlsI	GCNGC	5 cut(s)	121, 259, 460, 553, 841
Bme1390I	CCNGG	3 cut(s)	363, 858, 1095
Bme18I	GGWCC	1 cut(s)	1063
BmgT120I	GGNCC	2 cut(s)	18, 1063
BmiI	GGNNCC	4 cut(s)	328, 399, 762, 1231
BmrFI	CCNGG	3 cut(s)	363, 858, 1095
BmrI	ACTGGG	1 cut(s)	1065
BmsI	GCATC	5 cut(s)	430, 438, 606, 826, 931
BmuI	ACTGGG	1 cut(s)	1065
BpiI	GAAGAC	3 cut(s)	572, 1105, 1242
BpmI	CTGGAG	2 cut(s)	249, 1171
BpuEI	CTTGAG	1 cut(s)	980
BsaAI	YACGTR	1 cut(s)	1139
BsaBI	GATNNNNATC	1 cut(s)	282
BsaJI	CCNNGG	3 cut(s)	322, 362, 857
Bsc4I	CCNNNNNNNGG	3 cut(s)	313, 362, 982
Bse1I	ACTGG	5 cut(s)	232, 582, 1009, 1060, 1154
Bse21I	CCTNAGG	1 cut(s)	272
Bse3DI	GCAATG	1 cut(s)	552
Bse8I	GATNNNNATC	1 cut(s)	282
BseBI	CCWGG	3 cut(s)	363, 858, 1095
BseDI	CCNNGG	3 cut(s)	322, 362, 857
BseJI	GATNNNNATC	1 cut(s)	282
BseLI	CCNNNNNNNGG	3 cut(s)	313, 362, 982
BseMI	GCAATG	1 cut(s)	552
BseMII	CTCAG	2 cut(s)	600, 725
BseNI	ACTGG	5 cut(s)	232, 582, 1009, 1060, 1154
BseXI	GCAGC	5 cut(s)	106, 244, 470, 538, 851
BsgI	GTGCAG	1 cut(s)	537
Bsh1236I	CGCG	1 cut(s)	1218
BshFI	GGCC	2 cut(s)	20, 1093
BshNI	GGYRCC	1 cut(s)	326
BslI	CCNNNNNNNGG	3 cut(s)	313, 362, 982
BsmI	GAATGC	1 cut(s)	436
BsnI	GGCC	2 cut(s)	20, 1093
Bsp143I	GATC	9 cut(s)	76, 277, 397, 490, 729, 760, 931, 1120, 1131
Bsp68I	TCGCGA	1 cut(s)	1218
BspACI	CCGC	1 cut(s)	1087
BspANI	GGCC	2 cut(s)	20, 1093
BspCNI	CTCAG	2 cut(s)	601, 726
BspFNI	CGCG	1 cut(s)	1218
BspHI	TCATGA	2 cut(s)	196, 726
BspLI	GGNNCC	4 cut(s)	328, 399, 762, 1231
BspMAI	CTGCAG	1 cut(s)	1188
BspMI	ACCTGC	1 cut(s)	1177
BspT107I	GGYRCC	1 cut(s)	326
BspTI	CTTAAG	1 cut(s)	497
BsrDI	GCAATG	1 cut(s)	552
BsrI	ACTGG	5 cut(s)	232, 582, 1009, 1060, 1154
BssECI	CCNNGG	3 cut(s)	322, 362, 857
BssMI	GATC	9 cut(s)	76, 277, 397, 490, 729, 760, 931, 1120, 1131
BssSI	CACGAG	1 cut(s)	843
BssT1I	CCWWGG	1 cut(s)	322
Bst2BI	CACGAG	1 cut(s)	843
Bst2UI	CCWGG	3 cut(s)	363, 858, 1095
BstAFI	CTTAAG	1 cut(s)	497
BstAPI	GCANNNNNTGC	3 cut(s)	440, 464, 551
BstBAI	YACGTR	1 cut(s)	1139
BstC8I	GCNNGC	2 cut(s)	966, 1091
BstDEI	CTNAG	5 cut(s)	272, 529, 609, 734, 1128
BstFNI	CGCG	1 cut(s)	1218
BstH2I	RGCGCY	1 cut(s)	1158
BstHHI	GCGC	1 cut(s)	1157
BstKTI	GATC	9 cut(s)	79, 280, 400, 493, 732, 763, 934, 1123, 1134
BstMBI	GATC	9 cut(s)	76, 277, 397, 490, 729, 760, 931, 1120, 1131
BstMWI	GCNNNNNNNGC	4 cut(s)	440, 464, 551, 970
BstNI	CCWGG	3 cut(s)	363, 858, 1095
BstNSI	RCATGY	3 cut(s)	257, 591, 968
BstSCI	CCNGG	3 cut(s)	361, 856, 1093
BstSFI	CTRYAG	1 cut(s)	1184
BstUI	CGCG	1 cut(s)	1218
BstV1I	GCAGC	5 cut(s)	106, 244, 470, 538, 851
BstV2I	GAAGAC	3 cut(s)	572, 1105, 1242
BstX2I	RGATCY	4 cut(s)	76, 397, 760, 1131
BstXI	CCANNNNNNTGG	1 cut(s)	1240
BstYI	RGATCY	4 cut(s)	76, 397, 760, 1131
Bsu36I	CCTNAGG	1 cut(s)	272
BsuRI	GGCC	2 cut(s)	20, 1093
BtgZI	GCGATG	2 cut(s)	1199, 1233
BtsIMutI	CAGTG	2 cut(s)	927, 1123
BtuMI	TCGCGA	1 cut(s)	1218
BveI	ACCTGC	1 cut(s)	1177
Cac8I	GCNNGC	2 cut(s)	966, 1091
CciI	TCATGA	2 cut(s)	196, 726
CfoI	GCGC	1 cut(s)	1157
Cfr13I	GGNCC	2 cut(s)	18, 1063
Csp6I	GTAC	4 cut(s)	70, 585, 876, 1225
CspCI	CAANNNNNGTGG	2 cut(s)	1041, 1076
CviQI	GTAC	4 cut(s)	70, 585, 876, 1225
DdeI	CTNAG	5 cut(s)	272, 529, 609, 734, 1128
DpnI	GATC	9 cut(s)	78, 279, 399, 492, 731, 762, 933, 1122, 1133
DpnII	GATC	9 cut(s)	76, 277, 397, 490, 729, 760, 931, 1120, 1131
DraIII	CACNNNGTG	1 cut(s)	560
DrdI	GACNNNNNNGTC	1 cut(s)	722
DseDI	GACNNNNNNGTC	1 cut(s)	722
Eco130I	CCWWGG	1 cut(s)	322
Eco147I	AGGCCT	1 cut(s)	1093
Eco32I	GATATC	1 cut(s)	688
Eco47I	GGWCC	1 cut(s)	1063
Eco47III	AGCGCT	1 cut(s)	1156
Eco57I	CTGAAG	4 cut(s)	356, 648, 1099, 1128
Eco81I	CCTNAGG	1 cut(s)	272
EcoRI	GAATTC	1 cut(s)	709
EcoRII	CCWGG	3 cut(s)	361, 856, 1093
EcoRV	GATATC	1 cut(s)	688
EcoT14I	CCWWGG	1 cut(s)	322
EcoT22I	ATGCAT	2 cut(s)	436, 453
ErhI	CCWWGG	1 cut(s)	322
FalI	AAGNNNNNCTT	2 cut(s)	80, 112
FauI	CCCGC	1 cut(s)	1094
FauNDI	CATATG	3 cut(s)	469, 598, 658
Fnu4HI	GCNGC	5 cut(s)	120, 258, 459, 552, 840
Fsp4HI	GCNGC	5 cut(s)	120, 258, 459, 552, 840
FspBI	CTAG	2 cut(s)	221, 323
GlaI	GCGC	1 cut(s)	1156
GluI	GCNGC	5 cut(s)	120, 258, 459, 552, 840
GsuI	CTGGAG	2 cut(s)	249, 1171
HaeII	RGCGCY	1 cut(s)	1158
HaeIII	GGCC	2 cut(s)	20, 1093
HhaI	GCGC	1 cut(s)	1157
Hin6I	GCGC	1 cut(s)	1155
HinP1I	GCGC	1 cut(s)	1155
HinfI	GANTC	3 cut(s)	267, 987, 1180
HphI	GGTGA	3 cut(s)	614, 791, 1109
Hpy166II	GTNNAC	6 cut(s)	251, 412, 605, 806, 1063, 1142
Hpy188I	TCNGA	4 cut(s)	427, 715, 760, 895
Hpy8I	GTNNAC	6 cut(s)	251, 412, 605, 806, 1063, 1142
HpyAV	CCTTC	4 cut(s)	203, 1042, 1091, 1181
HpyCH4IV	ACGT	3 cut(s)	518, 948, 1138
HpyF10VI	GCNNNNNNNGC	4 cut(s)	440, 464, 551, 970
HpyF3I	CTNAG	5 cut(s)	272, 529, 609, 734, 1128
HpySE526I	ACGT	3 cut(s)	518, 948, 1138
HspAI	GCGC	1 cut(s)	1155
Kzo9I	GATC	9 cut(s)	76, 277, 397, 490, 729, 760, 931, 1120, 1131
LmnI	GCTCC	2 cut(s)	790, 1152
Lsp1109I	GCAGC	5 cut(s)	106, 244, 470, 538, 851
LweI	GCATC	5 cut(s)	430, 438, 606, 826, 931
MaeI	CTAG	2 cut(s)	221, 323
MaeII	ACGT	3 cut(s)	518, 948, 1138
MaeIII	GTNAC	5 cut(s)	208, 223, 289, 925, 1097
MalI	GATC	9 cut(s)	78, 279, 399, 492, 731, 762, 933, 1122, 1133
MboI	GATC	9 cut(s)	76, 277, 397, 490, 729, 760, 931, 1120, 1131
MboII	GAAGA	8 cut(s)	100, 577, 625, 641, 685, 695, 1105, 1242
MflI	RGATCY	4 cut(s)	76, 397, 760, 1131
MluCI	AATT	4 cut(s)	7, 108, 648, 709
MlyI	GAGTC	2 cut(s)	261, 1189
MmeI	TCCRAC	3 cut(s)	82, 738, 979
MnlI	CCTC	9 cut(s)	53, 78, 224, 317, 360, 535, 760, 866, 1030
Mph1103I	ATGCAT	2 cut(s)	436, 453
MseI	TTAA	6 cut(s)	6, 171, 498, 651, 951, 1023
MslI	CAYNNNNRTG	1 cut(s)	1218
MspA1I	CMGCKG	1 cut(s)	461
MspCI	CTTAAG	1 cut(s)	497
MspR9I	CCNGG	3 cut(s)	363, 858, 1095
Mva1269I	GAATGC	1 cut(s)	436
MvaI	CCWGG	3 cut(s)	363, 858, 1095
MvnI	CGCG	1 cut(s)	1218
MwoI	GCNNNNNNNGC	4 cut(s)	440, 464, 551, 970
NdeI	CATATG	3 cut(s)	469, 598, 658
NdeII	GATC	9 cut(s)	76, 277, 397, 490, 729, 760, 931, 1120, 1131
NlaIV	GGNNCC	4 cut(s)	328, 399, 762, 1231
NmuCI	GTSAC	2 cut(s)	925, 1097
NruI	TCGCGA	1 cut(s)	1218
NsiI	ATGCAT	2 cut(s)	436, 453
NspI	RCATGY	3 cut(s)	257, 591, 968
PaeI	GCATGC	1 cut(s)	968
PagI	TCATGA	2 cut(s)	196, 726
PceI	AGGCCT	1 cut(s)	1093
PciI	ACATGT	1 cut(s)	587
PctI	GAATGC	1 cut(s)	436
PfeI	GAWTC	1 cut(s)	987
PkrI	GCNGC	5 cut(s)	121, 259, 460, 553, 841
PleI	GAGTC	2 cut(s)	261, 1188
PpsI	GAGTC	2 cut(s)	261, 1188
Ppu21I	YACGTR	1 cut(s)	1139
PscI	ACATGT	1 cut(s)	587
PshBI	ATTAAT	1 cut(s)	651
Psp6I	CCWGG	3 cut(s)	361, 856, 1093
PspGI	CCWGG	3 cut(s)	361, 856, 1093
PspN4I	GGNNCC	4 cut(s)	328, 399, 762, 1231
PspPI	GGNCC	2 cut(s)	18, 1063
PstI	CTGCAG	1 cut(s)	1188
PsuI	RGATCY	4 cut(s)	76, 397, 760, 1131
PvuII	CAGCTG	1 cut(s)	461
RruI	TCGCGA	1 cut(s)	1218
RsaI	GTAC	4 cut(s)	71, 586, 877, 1226
RsaNI	GTAC	4 cut(s)	70, 585, 876, 1225
RseI	CAYNNNNRTG	1 cut(s)	1218
SaqAI	TTAA	6 cut(s)	6, 171, 498, 651, 951, 1023
SatI	GCNGC	5 cut(s)	120, 258, 459, 552, 840
Sau3AI	GATC	9 cut(s)	76, 277, 397, 490, 729, 760, 931, 1120, 1131
Sau96I	GGNCC	2 cut(s)	18, 1063
SchI	GAGTC	2 cut(s)	261, 1189
ScrFI	CCNGG	3 cut(s)	363, 858, 1095
SfaNI	GCATC	5 cut(s)	430, 438, 606, 826, 931
SfcI	CTRYAG	1 cut(s)	1184
SinI	GGWCC	1 cut(s)	1063
SmiMI	CAYNNNNRTG	1 cut(s)	1218
SmlI	CTYRAG	2 cut(s)	497, 959
SmoI	CTYRAG	2 cut(s)	497, 959
SphI	GCATGC	1 cut(s)	968
Sse9I	AATT	4 cut(s)	7, 108, 648, 709
SseBI	AGGCCT	1 cut(s)	1093
SsiI	CCGC	1 cut(s)	1087
SspI	AATATT	1 cut(s)	420
SspMI	CTAG	2 cut(s)	221, 323
StuI	AGGCCT	1 cut(s)	1093
StyD4I	CCNGG	3 cut(s)	361, 856, 1093
StyI	CCWWGG	1 cut(s)	322
TaiI	ACGT	3 cut(s)	521, 951, 1141
TaqI	TCGA	1 cut(s)	871
TasI	AATT	4 cut(s)	7, 108, 648, 709
TatI	WGTACW	1 cut(s)	584
TfiI	GAWTC	1 cut(s)	987
Tru1I	TTAA	6 cut(s)	6, 171, 498, 651, 951, 1023
Tru9I	TTAA	6 cut(s)	6, 171, 498, 651, 951, 1023
TscAI	CASTG	2 cut(s)	934, 1123
TseFI	GTSAC	2 cut(s)	925, 1097
TseI	GCWGC	5 cut(s)	119, 257, 458, 551, 839
Tsp45I	GTSAC	2 cut(s)	925, 1097
TspDTI	ATGAA	3 cut(s)	185, 213, 692
TspGWI	ACGGA	2 cut(s)	397, 1242
TspRI	CASTG	2 cut(s)	934, 1123
Vha464I	CTTAAG	1 cut(s)	497
VpaK11BI	GGWCC	1 cut(s)	1063
VspI	ATTAAT	1 cut(s)	651
XapI	RAATTY	2 cut(s)	108, 709
XceI	RCATGY	3 cut(s)	257, 591, 968
XmaJI	CCTAGG	1 cut(s)	322
XspI	CTAG	2 cut(s)	221, 323
Zsp2I	ATGCAT	2 cut(s)	436, 453

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.