Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0011118

Physical Location & Seq

Genomic Coordinates Reverse (-)

34549247 .. 34551811

Gene Structure 2565 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1252700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2565 bp

ATGGCGCTTCCGGTCAGGGAGATTTTTGGGATGCTATTTCTGGTGATTTTCTCTGCATTTGCAAGGATTGCAGAAGCTCAATCAAAATCTTTTCTGATAAATTGTGGCACAAATTCCAGCATCACTGTGAGTGGTAGGAAATGGGTTGGTGACTTAGCCCCCAACAACAACTTCACCCTCAGCTCCCCTACGAATGCTGCATCCACAACTCCCTCAAGTGTTGATTCAACAACATACGAGCCGCTGTACGCTACGGCGCGAATTTTCGATGGTGGCTTGAACTACACGTTCCCAGGTACTCGGGGGAGCTATTTTGTTAGGCTCCATTTCCGTCCTCTCCCATTTGATCATTACAATGTGAATGAATCTAGATTTGGAGTTGTTGCAAATGGTCTGAAGTTGGTGTCTGAATTCAGTGTTTCGGGTGAGATATCCCGAAAAAATGCGTACTTGCTGAGTTCAGGGAGCAATTTAACTTCTTTCTTGGTTAAGGAGTACATTTTGGCCATCAAGGAGGATGTACTTGTTTTTGAGTTCATCCCGGTTAAGGGGTCATTTGGATTCGTAAATGCGATTGAAATTGTTCCGGCGGTGGATACACTCTTTGCAGGATCAGTTAGTAAAGTGGGTGGAAATGGTGCAAATCAGAGTATAAGTTGGCAAGGGATTGAGACTATGTATAGGTTGAATGTTGGGGGTTCGGAAATTGATCCCGGTCAGGATGCAGATCTTTGGAGAACATGGGAAGTGGATTCTAGCTACATGATCACAGAAAATGCCGGGTTGAAAATCAATAACAGATCAAGCATTACCTTTTCGTCCGCAAATGATTCCTCGGTGGCGCCTCTTCTTGTATATGAAACTGCAAGAACTATGGTCAACACTGAAGTATTGGAGAAAAGGTTCAACATGTCGTGGAAGTTTGAAGTTCATCCTGATTTCGATTATTTAGTTCGACTGCATTTCTGTGAGCTGATTTTTGACGAAGCAAAAGAGAGGATCTTCAGAATTTACATAAACAACAGGACTGCAGCAGATAATTTCAATGTTTTTGTCCGTGCGGGGGGCAAGAACAAAGCGTATCATCAGGACTTTTTCGATGTGGTGTCTCCAAAAGTTGACACACTATGGATTCAATTGGGTCCTGACACAGCAGCTGGGGCTGCAGGAACTGATGCTCTCTTGAATGGCTTGGAGATTTTCAAGCTCAGTAGAAATGGGAATCTCGCTTATGTTGAGAAGTATGATTCAGGTGTTACTTCACCACGGAGTTCAAAAAATCAAATGCTTTGGGTGGGAGTTGGAGCAGGTATAGCTTCTGTTGCCATTCTTGCCATTGTCATTTACTGTTTCTGCAATAGACTGAGAGGAAAATCAACTGACACGAAAAACACCCCCGCTGGGTGGAGGCCGTTATTCCTTAATGGCTCCATTTTAAACAGCAATGTCAATGCCAAGGGAGCAGGAAGCCAGAATCCATATGGTTCTGCACCCTCTATCAGAGCTGGCAAGCGGTTCATGTTACCAGAGATCCGTGCAGCAACGAATAATTTTGATGAAAGTTTAGTGATTGGACTTGGAGGCTTTGGTAAGGTGTACAAAGGGGAGACTGATGATGGCACTCTTGTAGCAATCAAGCGAGCCAACCCCCAATCTCAGCAAGGACTGGCTGAATTTGAGACGGAGATTGAGATGCTTTCCAAGCTCAGGCATAGGCATTTGGTGTCATTGATTGGATTCTGTGAAGAACAAAATGAGATGATCTTGGTTTACGAGTACATGGCAAATGGGACACTTAGAAGCCATCTCTTTGGGAGTGATCTCCCACCGCTGACTTTTAAGCAACGAGTAGAAGCGTGCATTGGTGCTGCACGAGGACTCCATTATCTTCATACTGGAGCAGATAGGGGAATAATTCACAGGGATGTTAAGACAACCAACATACTGCTAGATGAAAATTTTGTCGCAAAAATGTCCGACTTTGGATTGTCTAAAACAGGTCCGGCTTTGGACCACACCCACGTTAGTACTGCAGTTAAAGGAAGCTTCGGATACCTTGACCCTGAATATTATCGACGACAGCAGTTGACAGAGAAATCGGATGTCTACTCGTTTGGTGTAGTGCTGTTTGAAGCTGTTTGTGCTAGGCCTGTTATAAACCCAACCTTGCCAAAAGATCAGATCAATCTTGCAGAATGGGCAATGATGTGGCAACGAAAGAGGTCCCTTGAGACCATCATTGACCGTCGTCTTGAAGGAACTTATTGTCCCGAGGCACTGAAGAAGTTTGGGGAGATAGCGGAGAAATGTCTCGCTGATGGGGGGAAGAGCCGGCCAACAATGGGGGAAGTTCTGTGGCACTTGGAGTATGCCTTGCAACTCCATGAAGCGTGGATGCGTACCAATGCTGGAGACAATTCTTTTACAAGTAGTCAGGCTTTCGGGGGAGAAGCACAAGAGAAGGAAGGAGCTCCGAGTTTTGATGAAGAGGCCGGTTGCAGCGGACAGTCTACCACAAGAGCATCCGATGAATCTGCAGACGGAGGTGCGAGGCATGAAAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009653 GO:0009719 GO:0009725 GO:0009741 GO:0009826 GO:0009987 GO:0010033 GO:0014070 GO:0016020 GO:0016043 GO:0016049 GO:0032502 GO:0032989 GO:0033993 GO:0040007 GO:0042221 GO:0044464 GO:0048589 GO:0048856 GO:0048869 GO:0050896 GO:0060560 GO:0071840 GO:0071944 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Malectin_like Pkinase_Tyr

Protein Analysis

855

Amino Acids

94.11

Weight (kDa)

5.98

Isoelectric Point (pI)

38.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Malectin	PF11721	31 - 147	8.1e-08	Malectin domain
Malectin_like	PF12819	33 - 402	6.8e-24	Malectin-like domain
Malectin	PF11721	279 - 379	2.9e-15	Malectin domain
PK_Tyr_Ser-Thr	PF07714	523 - 785	8.4e-47	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	523 - 787	8.3e-44	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0011118)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30570
fragaria_vesca	FvH4_3g10180
malus_domestica	MD01G1121700.v1.1 MD10G1252700.v1.1
prunus_persica	Prupe.4G090800_v2.0.a1
pyrus_communis	pycom05g25440 pycom10g20840
rosa_chinensis	RchiOBHm_Chr5g0016361
rosa_laevigata	RLG00000032246
rosa_multiflora	Rmu_sc0001235.1_g000034 Rmu_sc0012840.1_g000004 Rmu_sc0023137.1_g000012
rosa_roxburghii	Rroxscaffold_1G00060500 Rroxscaffold_1G00060510
rosa_rugosa	Rorug05G0031900
rosa_samantha	Rh5AG125400 Rh5BG124400 Rh5CG136600 Rh5DG124100
rosa_wichuraiana	Rw5G010870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2156
Acc36I	ACCTGC	1 cut(s)	1298
AccB1I	GGYRCC	1 cut(s)	841
AccI	GTMKAC	2 cut(s)	2106, 2510
AccII	CGCG	1 cut(s)	259
AciI	CCGC	9 cut(s)	242, 590, 822, 1061, 1398, 1513, 1829, 2300, 2502
AclWI	GGATC	4 cut(s)	619, 704, 1007, 1525
AcoI	YGGCCR	2 cut(s)	504, 2333
AcsI	RAATTY	6 cut(s)	112, 261, 410, 1008, 1673, 1957
AcuI	CTGAAG	4 cut(s)	416, 906, 988, 2300
AcyI	GRCGYC	1 cut(s)	842
AfaI	GTAC	9 cut(s)	248, 298, 449, 497, 522, 1598, 1778, 2029, 2401
AfiI	CCNNNNNNNGG	7 cut(s)	547, 548, 1063, 1401, 1404, 1707, 2342
AflIII	ACRYGT	2 cut(s)	285, 909
AjnI	CCWGG	1 cut(s)	292
AjuI	GAANNNNNNNTTGG	6 cut(s)	155, 187, 357, 389, 1845, 1877
AloI	GAACNNNNNNTCC	4 cut(s)	887, 919, 2251, 2283
Alw21I	GWGCWC	1 cut(s)	2473
Alw26I	GTCTC	7 cut(s)	665, 1113, 1601, 1673, 2225, 2315, 2406
AlwI	GGATC	4 cut(s)	619, 704, 1007, 1525
AlwNI	CAGNNNCTG	2 cut(s)	1157, 1172
Ama87I	CYCGRG	2 cut(s)	300, 2270
AoxI	GGCC	5 cut(s)	504, 1409, 2147, 2333, 2490
ApeKI	GCWGC	7 cut(s)	197, 1031, 1154, 1163, 1538, 1868, 2499
ApoI	RAATTY	6 cut(s)	112, 261, 410, 1008, 1673, 1957
Asp700I	GAANNNNTTC	2 cut(s)	582, 902
AspLEI	GCGC	3 cut(s)	7, 259, 844
AspS9I	GGNCC	4 cut(s)	1142, 2000, 2011, 2223
AsuC2I	CCSGG	3 cut(s)	542, 714, 781
AsuHPI	GGTGA	5 cut(s)	55, 161, 166, 437, 1254
AvaI	CYCGRG	2 cut(s)	300, 2270
AvaII	GGWCC	4 cut(s)	1142, 2000, 2011, 2223
BalI	TGGCCA	1 cut(s)	506
BanI	GGYRCC	1 cut(s)	841
BanII	GRGCYC	1 cut(s)	2473
BauI	CACGAG	1 cut(s)	1872
Bbv12I	GWGCWC	1 cut(s)	2473
BbvCI	CCTCAGC	1 cut(s)	179
BbvI	GCAGC	7 cut(s)	184, 1043, 1150, 1166, 1550, 1855, 2511
BccI	CCATC	6 cut(s)	263, 515, 1610, 1812, 2243, 2312
BceAI	ACGGC	2 cut(s)	270, 1396
BcgI	CGANNNNNNTGC	2 cut(s)	2516, 2550
BciT130I	CCWGG	1 cut(s)	294
BciVI	GTATCC	2 cut(s)	589, 2045
BclI	TGATCA	2 cut(s)	346, 765
BcnI	CCSGG	3 cut(s)	542, 714, 781
BcoDI	GTCTC	7 cut(s)	665, 1113, 1601, 1673, 2225, 2315, 2406
BfaI	CTAG	4 cut(s)	369, 756, 1949, 2145
BfmI	CTRYAG	4 cut(s)	1029, 1164, 2031, 2535
BfoI	RGCGCY	2 cut(s)	8, 845
BfuAI	ACCTGC	1 cut(s)	1298
BfuI	GTATCC	2 cut(s)	589, 2045
BglII	AGATCT	1 cut(s)	727
BisI	GCNGC	8 cut(s)	198, 242, 1032, 1155, 1164, 1539, 1869, 2500
BlsI	GCNGC	8 cut(s)	199, 243, 1033, 1156, 1165, 1540, 1870, 2501
BmcAI	AGTACT	1 cut(s)	2029
Bme1390I	CCNGG	4 cut(s)	294, 542, 714, 781
Bme18I	GGWCC	4 cut(s)	1142, 2000, 2011, 2223
BmeT110I	CYCGRG	2 cut(s)	300, 2270
BmgT120I	GGNCC	4 cut(s)	1142, 2000, 2011, 2223
BmiI	GGNNCC	5 cut(s)	323, 843, 1143, 1429, 2225
BmrFI	CCNGG	4 cut(s)	294, 542, 714, 781
BmsI	GCATC	8 cut(s)	21, 129, 209, 712, 1165, 1683, 2385, 2531
BoxI	GACNNNNGTC	1 cut(s)	2247
BpmI	CTGGAG	2 cut(s)	1917, 2430
Bpu10I	CCTNAGC	2 cut(s)	179, 1706
BpuEI	CTTGAG	2 cut(s)	199, 2249
BpuMI	CCSGG	3 cut(s)	542, 714, 781
BsaBI	GATNNNNATC	1 cut(s)	726
BsaHI	GRCGYC	1 cut(s)	842
BsaI	GGTCTC	1 cut(s)	2225
BsaJI	CCNNGG	5 cut(s)	292, 834, 1265, 1455, 2271
BsaWI	WCCGGW	1 cut(s)	10
BsaXI	ACNNNNNCTCC	4 cut(s)	887, 917, 1863, 1893
Bsc4I	CCNNNNNNNGG	7 cut(s)	547, 548, 1063, 1401, 1404, 1707, 2342
Bse118I	RCCGGY	2 cut(s)	2331, 2492
Bse1I	ACTGG	2 cut(s)	1671, 1900
Bse3DI	GCAATG	2 cut(s)	1450, 2208
Bse8I	GATNNNNATC	1 cut(s)	726
BseBI	CCWGG	1 cut(s)	294
BseDI	CCNNGG	5 cut(s)	292, 834, 1265, 1455, 2271
BseJI	GATNNNNATC	1 cut(s)	726
BseLI	CCNNNNNNNGG	7 cut(s)	547, 548, 1063, 1401, 1404, 1707, 2342
BseMI	GCAATG	2 cut(s)	1450, 2208
BseMII	CTCAG	6 cut(s)	193, 446, 1222, 1355, 1670, 1720
BseNI	ACTGG	2 cut(s)	1671, 1900
BseXI	GCAGC	7 cut(s)	184, 1043, 1150, 1166, 1550, 1855, 2511
BseYI	CCCAGC	2 cut(s)	1157, 1400
BsgI	GTGCAG	3 cut(s)	1473, 1557, 1854
Bsh1236I	CGCG	1 cut(s)	259
BshFI	GGCC	5 cut(s)	506, 1411, 2149, 2335, 2492
BshNI	GGYRCC	1 cut(s)	841
BsiHKAI	GWGCWC	1 cut(s)	2473
BsiHKCI	CYCGRG	2 cut(s)	300, 2270
BsiSI	CCGG	8 cut(s)	11, 542, 587, 714, 780, 2003, 2332, 2493
BslFI	GGGAC	3 cut(s)	1804, 2209, 2253
BslI	CCNNNNNNNGG	7 cut(s)	547, 548, 1063, 1401, 1404, 1707, 2342
BsmAI	GTCTC	7 cut(s)	665, 1113, 1601, 1673, 2225, 2315, 2406
BsmBI	CGTCTC	1 cut(s)	1673
BsmFI	GGGAC	3 cut(s)	1804, 2209, 2253
BsmI	GAATGC	1 cut(s)	199
BsnI	GGCC	5 cut(s)	506, 1411, 2149, 2335, 2492
Bso31I	GGTCTC	1 cut(s)	2225
BsoBI	CYCGRG	2 cut(s)	300, 2270
Bsp1286I	GDGCHC	1 cut(s)	2473
Bsp1407I	TGTACA	1 cut(s)	1596
BspACI	CCGC	9 cut(s)	242, 590, 822, 1061, 1398, 1513, 1829, 2300, 2502
BspANI	GGCC	5 cut(s)	506, 1411, 2149, 2335, 2492
BspCNI	CTCAG	6 cut(s)	192, 447, 1221, 1356, 1669, 1719
BspFNI	CGCG	1 cut(s)	259
BspLI	GGNNCC	5 cut(s)	323, 843, 1143, 1429, 2225
BspMAI	CTGCAG	4 cut(s)	1033, 1168, 2035, 2539
BspMI	ACCTGC	1 cut(s)	1298
BspPI	GGATC	4 cut(s)	619, 704, 1007, 1525
BspQI	GCTCTTC	1 cut(s)	2321
BspT107I	GGYRCC	1 cut(s)	841
BspTNI	GGTCTC	1 cut(s)	2225
BsrDI	GCAATG	2 cut(s)	1450, 2208
BsrFI	RCCGGY	2 cut(s)	2331, 2492
BsrGI	TGTACA	1 cut(s)	1596
BsrI	ACTGG	2 cut(s)	1671, 1900
BssAI	RCCGGY	2 cut(s)	2331, 2492
BssECI	CCNNGG	5 cut(s)	292, 834, 1265, 1455, 2271
BssNI	GRCGYC	1 cut(s)	842
BssSI	CACGAG	1 cut(s)	1872
BssT1I	CCWWGG	1 cut(s)	1455
Bst2BI	CACGAG	1 cut(s)	1872
Bst2UI	CCWGG	1 cut(s)	294
Bst4CI	ACNGT	4 cut(s)	127, 1349, 2246, 2508
Bst6I	CTCTTC	3 cut(s)	852, 2321, 2481
BstACI	GRCGYC	1 cut(s)	842
BstAPI	GCANNNNNTGC	1 cut(s)	68
BstAUI	TGTACA	1 cut(s)	1596
BstC8I	GCNNGC	5 cut(s)	1507, 1511, 1641, 1858, 2333
BstDEI	CTNAG	8 cut(s)	154, 179, 455, 1208, 1364, 1656, 1706, 1796
BstDSI	CCRYGG	1 cut(s)	1265
BstFNI	CGCG	1 cut(s)	259
BstH2I	RGCGCY	2 cut(s)	8, 845
BstHHI	GCGC	3 cut(s)	7, 259, 844
BstMAI	GTCTC	7 cut(s)	665, 1113, 1601, 1673, 2225, 2315, 2406
BstNI	CCWGG	1 cut(s)	294
BstNSI	RCATGY	1 cut(s)	913
BstPAI	GACNNNNGTC	1 cut(s)	2247
BstSCI	CCNGG	4 cut(s)	292, 540, 712, 779
BstSFI	CTRYAG	4 cut(s)	1029, 1164, 2031, 2535
BstUI	CGCG	1 cut(s)	259
BstV1I	GCAGC	7 cut(s)	184, 1043, 1150, 1166, 1550, 1855, 2511
BstX2I	RGATCY	3 cut(s)	727, 999, 1530
BstXI	CCANNNNNNTGG	1 cut(s)	1811
BstYI	RGATCY	3 cut(s)	727, 999, 1530
BsuI	GTATCC	2 cut(s)	589, 2045
BsuRI	GGCC	5 cut(s)	506, 1411, 2149, 2335, 2492
BtgI	CCRYGG	1 cut(s)	1265
BtsIMutI	CAGTG	4 cut(s)	123, 421, 882, 2276
BveI	ACCTGC	1 cut(s)	1298
Cac8I	GCNNGC	5 cut(s)	1507, 1511, 1641, 1858, 2333
CaiI	CAGNNNCTG	2 cut(s)	1157, 1172
CfoI	GCGC	3 cut(s)	7, 259, 844
Cfr10I	RCCGGY	2 cut(s)	2331, 2492
Cfr13I	GGNCC	4 cut(s)	1142, 2000, 2011, 2223
Csp6I	GTAC	9 cut(s)	247, 297, 448, 496, 521, 1597, 1777, 2028, 2400
CviAII	CATG	7 cut(s)	741, 763, 910, 1519, 1780, 2384, 2555
CviQI	GTAC	9 cut(s)	247, 297, 448, 496, 521, 1597, 1777, 2028, 2400
DdeI	CTNAG	8 cut(s)	154, 179, 455, 1208, 1364, 1656, 1706, 1796
DinI	GGCGCC	1 cut(s)	843
DraI	TTTAAA	1 cut(s)	1437
EaeI	YGGCCR	2 cut(s)	504, 2333
Eam1104I	CTCTTC	3 cut(s)	852, 2321, 2481
EarI	CTCTTC	3 cut(s)	852, 2321, 2481
Ecl136II	GAGCTC	1 cut(s)	2471
Eco130I	CCWWGG	1 cut(s)	1455
Eco147I	AGGCCT	1 cut(s)	2149
Eco24I	GRGCYC	1 cut(s)	2473
Eco31I	GGTCTC	1 cut(s)	2225
Eco32I	GATATC	1 cut(s)	432
Eco47I	GGWCC	4 cut(s)	1142, 2000, 2011, 2223
Eco53kI	GAGCTC	1 cut(s)	2471
Eco57I	CTGAAG	4 cut(s)	416, 906, 988, 2300
Eco88I	CYCGRG	2 cut(s)	300, 2270
EcoICRI	GAGCTC	1 cut(s)	2471
EcoO109I	RGGNCCY	2 cut(s)	1142, 2223
EcoRI	GAATTC	1 cut(s)	410
EcoRII	CCWGG	1 cut(s)	292
EcoRV	GATATC	1 cut(s)	432
EcoT14I	CCWWGG	1 cut(s)	1455
EcoT38I	GRGCYC	1 cut(s)	2473
EgeI	GGCGCC	1 cut(s)	843
EheI	GGCGCC	1 cut(s)	843
ErhI	CCWWGG	1 cut(s)	1455
Esp3I	CGTCTC	1 cut(s)	1673
FaeI	CATG	7 cut(s)	744, 766, 913, 1522, 1783, 2387, 2558
FaqI	GGGAC	3 cut(s)	1804, 2209, 2253
FatI	CATG	7 cut(s)	740, 762, 909, 1518, 1779, 2383, 2554
FauI	CCCGC	2 cut(s)	1054, 1405
FauNDI	CATATG	1 cut(s)	1480
FbaI	TGATCA	2 cut(s)	346, 765
FblI	GTMKAC	2 cut(s)	2106, 2510
Fnu4HI	GCNGC	8 cut(s)	198, 242, 1032, 1155, 1164, 1539, 1869, 2500
FriOI	GRGCYC	1 cut(s)	2473
Fsp4HI	GCNGC	8 cut(s)	198, 242, 1032, 1155, 1164, 1539, 1869, 2500
FspBI	CTAG	4 cut(s)	369, 756, 1949, 2145
GlaI	GCGC	3 cut(s)	6, 258, 843
GluI	GCNGC	8 cut(s)	198, 242, 1032, 1155, 1164, 1539, 1869, 2500
GsaI	CCCAGC	2 cut(s)	1161, 1404
GsuI	CTGGAG	2 cut(s)	1917, 2430
HaeII	RGCGCY	2 cut(s)	8, 845
HaeIII	GGCC	5 cut(s)	506, 1411, 2149, 2335, 2492
HapII	CCGG	8 cut(s)	11, 542, 587, 714, 780, 2003, 2332, 2493
HhaI	GCGC	3 cut(s)	7, 259, 844
Hin1I	GRCGYC	1 cut(s)	842
Hin1II	CATG	7 cut(s)	744, 766, 913, 1522, 1783, 2387, 2558
Hin6I	GCGC	3 cut(s)	5, 257, 842
HinP1I	GCGC	3 cut(s)	5, 257, 842
HincII	GTYRAC	3 cut(s)	880, 1120, 2088
HindII	GTYRAC	3 cut(s)	880, 1120, 2088
HindIII	AAGCTT	1 cut(s)	2044
HpaII	CCGG	8 cut(s)	11, 542, 587, 714, 780, 2003, 2332, 2493
HphI	GGTGA	5 cut(s)	55, 161, 166, 437, 1254
Hpy166II	GTNNAC	7 cut(s)	880, 1120, 1597, 1771, 2088, 2107, 2511
Hpy188III	TCNNGA	9 cut(s)	369, 435, 719, 935, 1088, 1145, 1183, 2252, 2270
Hpy8I	GTNNAC	7 cut(s)	880, 1120, 1597, 1771, 2088, 2107, 2511
Hpy99I	CGWCG	2 cut(s)	2079, 2250
HpyAV	CCTTC	3 cut(s)	2249, 2455, 2459
HpyCH4III	ACNGT	4 cut(s)	127, 1349, 2246, 2508
HpyCH4IV	ACGT	2 cut(s)	287, 2022
HpyF3I	CTNAG	8 cut(s)	154, 179, 455, 1208, 1364, 1656, 1706, 1796
HpySE526I	ACGT	2 cut(s)	287, 2022
Hsp92I	GRCGYC	1 cut(s)	842
Hsp92II	CATG	7 cut(s)	744, 766, 913, 1522, 1783, 2387, 2558
HspAI	GCGC	3 cut(s)	5, 257, 842
KasI	GGCGCC	1 cut(s)	841
KroI	GCCGGC	1 cut(s)	2331
KroNI	GCCGGC	1 cut(s)	2333
Ksp22I	TGATCA	2 cut(s)	346, 765
LguI	GCTCTTC	1 cut(s)	2321
Lsp1109I	GCAGC	7 cut(s)	184, 1043, 1150, 1166, 1550, 1855, 2511
LweI	GCATC	8 cut(s)	21, 129, 209, 712, 1165, 1683, 2385, 2531
MaeI	CTAG	4 cut(s)	369, 756, 1949, 2145
MaeII	ACGT	2 cut(s)	287, 2022
MaeIII	GTNAC	3 cut(s)	149, 1255, 1521
MboII	GAAGA	7 cut(s)	839, 994, 1757, 1880, 2293, 2338, 2498
MfeI	CAATTG	1 cut(s)	1136
MflI	RGATCY	3 cut(s)	727, 999, 1530
MhlI	GDGCHC	1 cut(s)	2473
MlsI	TGGCCA	1 cut(s)	506
MluNI	TGGCCA	1 cut(s)	506
Mly113I	GGCGCC	1 cut(s)	842
MlyI	GAGTC	1 cut(s)	1872
MmeI	TCCRAC	2 cut(s)	1282, 2001
Mox20I	TGGCCA	1 cut(s)	506
MroNI	GCCGGC	1 cut(s)	2331
MroXI	GAANNNNTTC	2 cut(s)	582, 902
MscI	TGGCCA	1 cut(s)	506
MseI	TTAA	8 cut(s)	473, 489, 546, 1422, 1436, 1839, 1929, 2037
MslI	CAYNNNNRTG	4 cut(s)	125, 354, 966, 1923
Msp20I	TGGCCA	1 cut(s)	506
MspA1I	CMGCKG	5 cut(s)	244, 1157, 1400, 1831, 2502
MspI	CCGG	8 cut(s)	11, 542, 587, 714, 780, 2003, 2332, 2493
MspR9I	CCNGG	4 cut(s)	294, 542, 714, 781
MunI	CAATTG	1 cut(s)	1136
Mva1269I	GAATGC	1 cut(s)	199
MvaI	CCWGG	1 cut(s)	294
MvnI	CGCG	1 cut(s)	259
NaeI	GCCGGC	1 cut(s)	2333
NarI	GGCGCC	1 cut(s)	842
NciI	CCSGG	3 cut(s)	542, 714, 781
NdeI	CATATG	1 cut(s)	1480
NgoMIV	GCCGGC	1 cut(s)	2331
NlaIII	CATG	7 cut(s)	744, 766, 913, 1522, 1783, 2387, 2558
NlaIV	GGNNCC	5 cut(s)	323, 843, 1143, 1429, 2225
NmuCI	GTSAC	1 cut(s)	149
NspI	RCATGY	1 cut(s)	913
PceI	AGGCCT	1 cut(s)	2149
PciI	ACATGT	1 cut(s)	909
PciSI	GCTCTTC	1 cut(s)	2321
PctI	GAATGC	1 cut(s)	199
PdiI	GCCGGC	1 cut(s)	2333
PdmI	GAANNNNTTC	2 cut(s)	582, 902
PkrI	GCNGC	8 cut(s)	199, 243, 1033, 1156, 1165, 1540, 1870, 2501
PleI	GAGTC	1 cut(s)	1872
PluTI	GGCGCC	1 cut(s)	845
PpsI	GAGTC	1 cut(s)	1872
PpuMI	RGGWCCY	2 cut(s)	1142, 2223
PscI	ACATGT	1 cut(s)	909
PshAI	GACNNNNGTC	1 cut(s)	2247
PsiI	TTATAA	1 cut(s)	2156
Psp124BI	GAGCTC	1 cut(s)	2473
Psp5II	RGGWCCY	2 cut(s)	1142, 2223
Psp6I	CCWGG	1 cut(s)	292
PspFI	CCCAGC	2 cut(s)	1157, 1400
PspGI	CCWGG	1 cut(s)	292
PspN4I	GGNNCC	5 cut(s)	323, 843, 1143, 1429, 2225
PspPI	GGNCC	4 cut(s)	1142, 2000, 2011, 2223
PspPPI	RGGWCCY	2 cut(s)	1142, 2223
PstI	CTGCAG	4 cut(s)	1033, 1168, 2035, 2539
PstNI	CAGNNNCTG	2 cut(s)	1157, 1172
PsuI	RGATCY	3 cut(s)	727, 999, 1530
PvuII	CAGCTG	1 cut(s)	1157
RsaI	GTAC	9 cut(s)	248, 298, 449, 497, 522, 1598, 1778, 2029, 2401
RsaNI	GTAC	9 cut(s)	247, 297, 448, 496, 521, 1597, 1777, 2028, 2400
RseI	CAYNNNNRTG	4 cut(s)	125, 354, 966, 1923
SacI	GAGCTC	1 cut(s)	2473
SapI	GCTCTTC	1 cut(s)	2321
SaqAI	TTAA	8 cut(s)	473, 489, 546, 1422, 1436, 1839, 1929, 2037
SatI	GCNGC	8 cut(s)	198, 242, 1032, 1155, 1164, 1539, 1869, 2500
Sau96I	GGNCC	4 cut(s)	1142, 2000, 2011, 2223
ScaI	AGTACT	1 cut(s)	2029
SchI	GAGTC	1 cut(s)	1872
ScrFI	CCNGG	4 cut(s)	294, 542, 714, 781
SduI	GDGCHC	1 cut(s)	2473
SfaNI	GCATC	8 cut(s)	21, 129, 209, 712, 1165, 1683, 2385, 2531
SfcI	CTRYAG	4 cut(s)	1029, 1164, 2031, 2535
SfoI	GGCGCC	1 cut(s)	843
SinI	GGWCC	4 cut(s)	1142, 2000, 2011, 2223
SmiMI	CAYNNNNRTG	4 cut(s)	125, 354, 966, 1923
SmlI	CTYRAG	2 cut(s)	214, 2228
SmoI	CTYRAG	2 cut(s)	214, 2228
SseBI	AGGCCT	1 cut(s)	2149
SsiI	CCGC	9 cut(s)	242, 590, 822, 1061, 1398, 1513, 1829, 2300, 2502
SspDI	GGCGCC	1 cut(s)	841
SspI	AATATT	1 cut(s)	2069
SspMI	CTAG	4 cut(s)	369, 756, 1949, 2145
SstI	GAGCTC	1 cut(s)	2473
StuI	AGGCCT	1 cut(s)	2149
StyD4I	CCNGG	4 cut(s)	292, 540, 712, 779
StyI	CCWWGG	1 cut(s)	1455
TaaI	ACNGT	4 cut(s)	127, 1349, 2246, 2508
TaiI	ACGT	2 cut(s)	290, 2025
TaqI	TCGA	5 cut(s)	267, 942, 955, 1098, 2074
TatI	WGTACW	5 cut(s)	495, 520, 1596, 1776, 2027
TauI	GCSGC	1 cut(s)	244
Tru1I	TTAA	8 cut(s)	473, 489, 546, 1422, 1436, 1839, 1929, 2037
Tru9I	TTAA	8 cut(s)	473, 489, 546, 1422, 1436, 1839, 1929, 2037
TscAI	CASTG	4 cut(s)	130, 421, 889, 2283
TseFI	GTSAC	1 cut(s)	149
TseI	GCWGC	7 cut(s)	197, 1031, 1154, 1163, 1538, 1868, 2499
Tsp45I	GTSAC	1 cut(s)	149
TspGWI	ACGGA	6 cut(s)	320, 1046, 1282, 1523, 1697, 2556
TspRI	CASTG	4 cut(s)	130, 421, 889, 2283
VpaK11BI	GGWCC	4 cut(s)	1142, 2000, 2011, 2223
XapI	RAATTY	6 cut(s)	112, 261, 410, 1008, 1673, 1957
XbaI	TCTAGA	1 cut(s)	368
XceI	RCATGY	1 cut(s)	913
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1478
XmiI	GTMKAC	2 cut(s)	2106, 2510
XmnI	GAANNNNTTC	2 cut(s)	582, 902
XspI	CTAG	4 cut(s)	369, 756, 1949, 2145
ZrmI	AGTACT	1 cut(s)	2029

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.