RLG00000032246

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0011118
Physical Location & Seq
Forward (+)
12565848 .. 12568400
2553 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032246

Sequence Viewer

Length: 2553 bp
ATGGAGAACTTTGGGCTGTTTTCTCTGGTGATAGTCTTTGTGTTTTCGAAGACTAATGAAGCTCAATCAAGGTCTTTTTTGATAAACTGTGGTACAAATTCCAGTGTCACTGTGAGTGGTAGGAAATGGGTCGGTGATTCATCCCCTAACAATAACATCACTCTCAGTTCCAGTGGAATTCCTGCCTCCACTGATGTCAAAAGTGTTGATGATTCGACATATGGGGAGCTCTATAAAACAGCTCGAATGTTCGAGAGTAGCTTGAACTACACATTCCAAGGTGTTAGGGGAGACTATTTTGTTAGGCTCCATTTCAGTCCTTTCTCCTTTGATGACTACAATGTAAATGTGTCTTCGTTTGGAGTTGTGGCAAATGGTCTGAAACTGGTTGAGAAATTCGAGGTTCCAGGTGAGTTATTGCACAAGAATGCATACCTGCAGGGTTCAGCAAGCAATTCAACTTCTTCAATAGTCAAGGAGTATGTTTTGGCAATCAATAGGGATGCACTAGTCATTGAGTTCATCCCGGATAAGGAGACATTCGGGTTTGTTAATGCAATCGAGATTGTTCCAGCTGTAGATGACCTCTTTGCAGGATCAGCTAGTAAAGTGGGTGGAAATGGTGCAAATGTGAATGTAAGTTGGCAGGGGATCGAAACTATATACAGGTTGAATGTTGGTGGTTCTGAAGTTAGTCCCAGTCAGGATTCAGATCTTTGGAGAAAATGGGAGATGGATACTAGCTACATGATCACAGCAAATGCCGGGGTAGAAATCAAAAACAGTTCCAATATTACCTATTCTTCTGTGAATGATTCCTCACTGGCTCCTCTTGTAGTCTATGAGAGTGCAAGAACTATGTCCAACAATGAAGTCTTGGAGAAAAGGTTCAACATGTCATGGAAGTTTGAAGTGGATCCTGGTTTTGATTATTTGATCCGATTACATTTCTGTGAGCTAGTTTTTGACAAGGCAAACCAAAGGATATTTAGAGTCTACATAAACAACAGGACTGCAGCAGATAATTACAATGTGTTCGTTCGAGCAGGAGGGATGAACAAAGCATATCATCAGGATTTCTTTGATGTGGTGTCTTCAAAAGTCAAGACACTGTGGATTCAGTTGGGTCCTGACACAAATGTTGGGGCTGCAGGAACTGATGCACTATTGAATGGCTTGGAGATTTTCAAGATCAGCCGAAATGGGAATCTTGCTTATGTGGACAACTATGAGTCGAGTGCCAATTCATCTAAAGCAAGTTCGAAAAATGCAATTCTTTGGGGGGCAATTGGAGCGGGTGTAGCTACTGTTGCCATTCTAGCCACTCTAATTTTTTGTTTCTGCAAATGCCGAAGAGAAAAATCAAGTGACACCAAAACCAATCCCCCTGGATGGAGGCCATTGTTCCTTGTAAACAGCACTGTCAATGCCAAGGGATCAGCAGGAAGCAAGAATCAGTATGGTTCTGTGGCCTCTACGAGAGCTGGCAAGCGGTTCTTGTTACCTGAGATTCGTGCAGCAACTAATAATTTTGATGAAAGTTTAGTGATTGGAGTTGGAGGCTTTGGAAAGGTCTACAAGGGTGAGATTGAGGATGGCACTCTTGTAGCAATCAAGCGGTCCAACCCCCAATCCCAGCAAGGCCTGGCTGAATTCGAGACGGAGATTGAGATGCTTTCAAAGCTCAGGCATAGGCATTTAGTGTCCTTGATTGGATTCTGTGATGAGCAAAATGAGATGATCTTGGTTTATGAGTATATGGCAAATGGGACTCTCAGAAGTCATCTCTTTGGAAGCGAACTCCCACCGTTGACGTGGAAGCAACGAGTAGAAGCATGCATTGGCGCTGCTCGAGGACTACATTACCTTCATACCGGAGCAGATAGGGGGGTAATCCACAGGGATGTTAAGACAACCAACATACTGCTAGATGAGAACTTTGTAGCGAAGATGTCCGACTTTGGACTATCGAAAACTGGTCCTGCTTTGGACCATACCCATGTTAGTACTGCAGTTAAAGGAAGCTTTGGATATCTAGATCCTGAGTATTTTCGACGACAGCAGTTGACAGAGAAATCGGATGTCTACTCTTTTGGTGTAGTGTTGTTTGAAGCTGTTTGTTCTCGGCCAGTCATAAACCCAACCTTGCCAAAAGATCAGATCAATCTTGCAGAATGGGCAATGAGATGGCAGAGGCAGAGGTCACTTGAAACTATCATCGATCCTCGTCTTGAAGGAAACTACTGTCCTGAGTCCTTGAAGAAGTTTGGGGACATAGCAGAGAAATGCCTTGCTGATGAGGGGAAGAGCCGGCCAACGATGGGGGAAGTTTTGTGGCACTTGGAGTATGTCTTGCAACTCCACGAAGCATGGATCCGGAAAAATGCAGGAGATAATTCTTTCACAGGTAGTCAAGGATTTGGGGGTTTAGCTGTGGATGAAGATGAAGCAGAAGAGAGGGAAGCCACCAGTTTCGATGAAGAGACTGGTTTCAGCAGAGAGTCTACTAGTAGAGGACTTGATGAATCTACTACAGAAGGCAGAAAGAGTTGA

Protein Analysis

851

Amino Acids

94.05

Weight (kDa)

5.54

Isoelectric Point (pI)

38.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin PF11721 26 - 187 3.2e-10 Malectin domain
Malectin_like PF12819 28 - 398 2.5e-27 Malectin-like domain
Malectin PF11721 221 - 376 4.8e-14 Malectin domain
PK_Tyr_Ser-Thr PF07714 516 - 779 3.3e-47 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 516 - 780 1.6e-44 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 444
AccBSI CCGCTC 1 cut(s) 1295
AccI GTMKAC 4 cut(s) 996, 1575, 2085, 2504
AccIII TCCGGA 1 cut(s) 2376
AciI CCGC 3 cut(s) 1295, 1492, 1618
AcoI YGGCCR 2 cut(s) 2126, 2312
AcsI RAATTY 4 cut(s) 97, 177, 395, 1652
AcuI CTGAAG 1 cut(s) 708
AfaI GTAC 2 cut(s) 94, 2008
AfiI CCNNNNNNNGG 3 cut(s) 532, 1392, 2321
AflIII ACRYGT 1 cut(s) 894
AhlI ACTAGT 2 cut(s) 508, 2507
AjiI CACGTC 1 cut(s) 1815
AjnI CCWGG 4 cut(s) 406, 919, 1387, 1644
AjuI GAANNNNNNNTTGG 2 cut(s) 1824, 1856
AloI GAACNNNNNNTCC 2 cut(s) 872, 904
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 4 cut(s) 285, 530, 1652, 2477
AlwNI CAGNNNCTG 1 cut(s) 1157
Ama87I CYCGRG 1 cut(s) 1851
Aor13HI TCCGGA 1 cut(s) 2376
AoxI GGCC 5 cut(s) 1397, 1470, 1642, 2126, 2312
ApeKI GCWGC 4 cut(s) 1016, 1148, 1517, 1847
ApoI RAATTY 4 cut(s) 97, 177, 395, 1652
Asp700I GAANNNNTTC 1 cut(s) 887
AspLEI GCGC 1 cut(s) 1847
AspS9I GGNCC 4 cut(s) 1127, 1620, 1979, 1990
AsuC2I CCSGG 2 cut(s) 527, 766
AsuHPI GGTGA 4 cut(s) 40, 146, 422, 1595
AsuII TTCGAA 2 cut(s) 47, 1262
AvaI CYCGRG 1 cut(s) 1851
AvaII GGWCC 4 cut(s) 1127, 1620, 1979, 1990
BamHI GGATCC 2 cut(s) 916, 2373
BanII GRGCYC 1 cut(s) 231
BbsI GAAGAC 3 cut(s) 56, 345, 1086
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 4 cut(s) 1028, 1135, 1529, 1834
BccI CCATC 5 cut(s) 727, 1386, 1589, 2181, 2314
BciT130I CCWGG 4 cut(s) 408, 921, 1389, 1646
BciVI GTATCC 1 cut(s) 730
BclI TGATCA 1 cut(s) 750
BcnI CCSGG 2 cut(s) 527, 766
BcoDI GTCTC 4 cut(s) 285, 530, 1652, 2477
BcuI ACTAGT 2 cut(s) 508, 2507
BfaI CTAG 8 cut(s) 509, 603, 741, 959, 1319, 1928, 2036, 2508
BfmI CTRYAG 6 cut(s) 437, 576, 1014, 1149, 2010, 2532
BfoI RGCGCY 1 cut(s) 1848
BfuAI ACCTGC 1 cut(s) 444
BfuI GTATCC 1 cut(s) 730
BglII AGATCT 1 cut(s) 712
BisI GCNGC 4 cut(s) 1017, 1149, 1518, 1848
BlsI GCNGC 4 cut(s) 1018, 1150, 1519, 1849
BmcAI AGTACT 1 cut(s) 2008
Bme1390I CCNGG 6 cut(s) 408, 527, 766, 921, 1389, 1646
Bme18I GGWCC 4 cut(s) 1127, 1620, 1979, 1990
BmeT110I CYCGRG 1 cut(s) 1851
BmgBI CACGTC 1 cut(s) 1815
BmgT120I GGNCC 4 cut(s) 1127, 1620, 1979, 1990
BmiI GGNNCC 6 cut(s) 308, 405, 828, 918, 1128, 2375
BmrFI CCNGG 6 cut(s) 408, 527, 766, 921, 1389, 1646
BmrI ACTGGG 1 cut(s) 693
BmsI GCATC 3 cut(s) 493, 1150, 1662
BmuI ACTGGG 1 cut(s) 693
BpiI GAAGAC 3 cut(s) 56, 345, 1086
Bpu10I CCTNAGC 1 cut(s) 1685
Bpu14I TTCGAA 2 cut(s) 47, 1262
BpuMI CCSGG 2 cut(s) 527, 766
Bsa29I ATCGAT 1 cut(s) 2220
BsaBI GATNNNNATC 1 cut(s) 711
BsaJI CCNNGG 4 cut(s) 277, 765, 1387, 1431
BsaWI WCCGGW 2 cut(s) 1874, 2376
BsaXI ACNNNNNCTCC 4 cut(s) 872, 902, 1284, 1314
Bsc4I CCNNNNNNNGG 3 cut(s) 532, 1392, 2321
Bse118I RCCGGY 1 cut(s) 2310
Bse1I ACTGG 9 cut(s) 102, 171, 390, 699, 828, 1981, 2129, 2469, 2491
Bse3DI GCAATG 1 cut(s) 2187
Bse8I GATNNNNATC 1 cut(s) 711
BseAI TCCGGA 1 cut(s) 2376
BseBI CCWGG 4 cut(s) 408, 921, 1389, 1646
BseCI ATCGAT 1 cut(s) 2220
BseDI CCNNGG 4 cut(s) 277, 765, 1387, 1431
BseGI GGATG 9 cut(s) 140, 508, 522, 1059, 1397, 1600, 1909, 2086, 2443
BseJI GATNNNNATC 1 cut(s) 711
BseLI CCNNNNNNNGG 3 cut(s) 532, 1392, 2321
BseMI GCAATG 1 cut(s) 2187
BseMII CTCAG 6 cut(s) 178, 1497, 1699, 1789, 2034, 2241
BseNI ACTGG 9 cut(s) 102, 171, 390, 699, 828, 1981, 2129, 2469, 2491
BseRI GAGGAG 1 cut(s) 819
BseXI GCAGC 4 cut(s) 1028, 1135, 1529, 1834
BseYI CCCAGC 1 cut(s) 1635
BsgI GTGCAG 1 cut(s) 1536
BshFI GGCC 5 cut(s) 1399, 1472, 1644, 2128, 2314
BshVI ATCGAT 1 cut(s) 2220
BsiHKAI GWGCWC 1 cut(s) 231
BsiHKCI CYCGRG 1 cut(s) 1851
BsiSI CCGG 5 cut(s) 527, 765, 1875, 2311, 2377
BslFI GGGAC 3 cut(s) 681, 1783, 2285
BslI CCNNNNNNNGG 3 cut(s) 532, 1392, 2321
BsmAI GTCTC 4 cut(s) 285, 530, 1652, 2477
BsmBI CGTCTC 1 cut(s) 1652
BsmFI GGGAC 3 cut(s) 681, 1783, 2285
BsmI GAATGC 1 cut(s) 433
BsnI GGCC 5 cut(s) 1399, 1472, 1644, 2128, 2314
BsoBI CYCGRG 1 cut(s) 1851
Bsp119I TTCGAA 2 cut(s) 47, 1262
Bsp1286I GDGCHC 1 cut(s) 231
Bsp13I TCCGGA 1 cut(s) 2376
BspACI CCGC 3 cut(s) 1295, 1492, 1618
BspANI GGCC 5 cut(s) 1399, 1472, 1644, 2128, 2314
BspCNI CTCAG 6 cut(s) 177, 1498, 1698, 1788, 2035, 2242
BspDI ATCGAT 1 cut(s) 2220
BspEI TCCGGA 1 cut(s) 2376
BspLI GGNNCC 6 cut(s) 308, 405, 828, 918, 1128, 2375
BspMAI CTGCAG 4 cut(s) 441, 1018, 1153, 2014
BspMI ACCTGC 1 cut(s) 444
BspQI GCTCTTC 1 cut(s) 2300
BspT104I TTCGAA 2 cut(s) 47, 1262
BsrBI CCGCTC 1 cut(s) 1295
BsrDI GCAATG 1 cut(s) 2187
BsrFI RCCGGY 1 cut(s) 2310
BsrI ACTGG 9 cut(s) 102, 171, 390, 699, 828, 1981, 2129, 2469, 2491
BssAI RCCGGY 1 cut(s) 2310
BssECI CCNNGG 4 cut(s) 277, 765, 1387, 1431
BssT1I CCWWGG 2 cut(s) 277, 1431
Bst2UI CCWGG 4 cut(s) 408, 921, 1389, 1646
Bst4CI ACNGT 8 cut(s) 89, 112, 785, 1113, 1309, 1423, 1809, 2246
Bst6I CTCTTC 4 cut(s) 1348, 2300, 2448, 2475
BstBI TTCGAA 2 cut(s) 47, 1262
BstC8I GCNNGC 5 cut(s) 451, 1486, 1490, 1837, 2312
BstDEI CTNAG 6 cut(s) 164, 1506, 1685, 1775, 2043, 2250
BstF5I GGATG 9 cut(s) 140, 508, 522, 1059, 1397, 1600, 1909, 2086, 2443
BstH2I RGCGCY 1 cut(s) 1848
BstHHI GCGC 1 cut(s) 1847
BstMAI GTCTC 4 cut(s) 285, 530, 1652, 2477
BstMWI GCNNNNNNNGC 7 cut(s) 599, 1292, 1301, 1310, 1319, 1681, 2177
BstNI CCWGG 4 cut(s) 408, 921, 1389, 1646
BstNSI RCATGY 2 cut(s) 898, 1839
BstSCI CCNGG 6 cut(s) 406, 525, 764, 919, 1387, 1644
BstSFI CTRYAG 6 cut(s) 437, 576, 1014, 1149, 2010, 2532
BstV1I GCAGC 4 cut(s) 1028, 1135, 1529, 1834
BstV2I GAAGAC 3 cut(s) 56, 345, 1086
BstX2I RGATCY 4 cut(s) 712, 916, 2038, 2373
BstYI RGATCY 4 cut(s) 712, 916, 2038, 2373
Bsu15I ATCGAT 1 cut(s) 2220
BsuI GTATCC 1 cut(s) 730
BsuRI GGCC 5 cut(s) 1399, 1472, 1644, 2128, 2314
BsuTUI ATCGAT 1 cut(s) 2220
BtrI CACGTC 1 cut(s) 1815
BtsCI GGATG 9 cut(s) 140, 508, 522, 1059, 1397, 1600, 1909, 2086, 2443
BtsIMutI CAGTG 7 cut(s) 108, 109, 178, 189, 821, 1109, 1419
BveI ACCTGC 1 cut(s) 444
Cac8I GCNNGC 5 cut(s) 451, 1486, 1490, 1837, 2312
CaiI CAGNNNCTG 1 cut(s) 1157
CfoI GCGC 1 cut(s) 1847
Cfr10I RCCGGY 1 cut(s) 2310
Cfr13I GGNCC 4 cut(s) 1127, 1620, 1979, 1990
ClaI ATCGAT 1 cut(s) 2220
Csp6I GTAC 2 cut(s) 93, 2007
CviAII CATG 6 cut(s) 748, 895, 900, 1836, 2000, 2370
CviQI GTAC 2 cut(s) 93, 2007
DdeI CTNAG 6 cut(s) 164, 1506, 1685, 1775, 2043, 2250
EaeI YGGCCR 2 cut(s) 2126, 2312
Eam1104I CTCTTC 4 cut(s) 1348, 2300, 2448, 2475
EarI CTCTTC 4 cut(s) 1348, 2300, 2448, 2475
Ecl136II GAGCTC 1 cut(s) 229
Eco130I CCWWGG 2 cut(s) 277, 1431
Eco147I AGGCCT 1 cut(s) 1644
Eco24I GRGCYC 1 cut(s) 231
Eco32I GATATC 1 cut(s) 2033
Eco47I GGWCC 4 cut(s) 1127, 1620, 1979, 1990
Eco53kI GAGCTC 1 cut(s) 229
Eco57I CTGAAG 1 cut(s) 708
Eco88I CYCGRG 1 cut(s) 1851
EcoICRI GAGCTC 1 cut(s) 229
EcoO109I RGGNCCY 1 cut(s) 1127
EcoRI GAATTC 2 cut(s) 177, 1652
EcoRII CCWGG 4 cut(s) 406, 919, 1387, 1644
EcoRV GATATC 1 cut(s) 2033
EcoT14I CCWWGG 2 cut(s) 277, 1431
EcoT22I ATGCAT 2 cut(s) 433, 1841
EcoT38I GRGCYC 1 cut(s) 231
ErhI CCWWGG 2 cut(s) 277, 1431
Esp3I CGTCTC 1 cut(s) 1652
FaeI CATG 6 cut(s) 751, 898, 903, 1839, 2003, 2373
FalI AAGNNNNNCTT 2 cut(s) 1481, 1513
FaqI GGGAC 3 cut(s) 681, 1783, 2285
FatI CATG 6 cut(s) 747, 894, 899, 1835, 1999, 2369
FauI CCCGC 1 cut(s) 1288
FauNDI CATATG 1 cut(s) 220
FbaI TGATCA 1 cut(s) 750
FblI GTMKAC 4 cut(s) 996, 1575, 2085, 2504
Fnu4HI GCNGC 4 cut(s) 1017, 1149, 1518, 1848
FokI GGATG 9 cut(s) 127, 509, 515, 1066, 1404, 1607, 1916, 2093, 2450
FriOI GRGCYC 1 cut(s) 231
Fsp4HI GCNGC 4 cut(s) 1017, 1149, 1518, 1848
FspBI CTAG 8 cut(s) 509, 603, 741, 959, 1319, 1928, 2036, 2508
GlaI GCGC 1 cut(s) 1846
GluI GCNGC 4 cut(s) 1017, 1149, 1518, 1848
GsaI CCCAGC 1 cut(s) 1639
HaeII RGCGCY 1 cut(s) 1848
HaeIII GGCC 5 cut(s) 1399, 1472, 1644, 2128, 2314
HapII CCGG 5 cut(s) 527, 765, 1875, 2311, 2377
HhaI GCGC 1 cut(s) 1847
Hin1II CATG 6 cut(s) 751, 898, 903, 1839, 2003, 2373
Hin6I GCGC 1 cut(s) 1845
HinP1I GCGC 1 cut(s) 1845
HincII GTYRAC 2 cut(s) 1812, 2067
HindII GTYRAC 2 cut(s) 1812, 2067
HindIII AAGCTT 1 cut(s) 2023
HpaII CCGG 5 cut(s) 527, 765, 1875, 2311, 2377
HphI GGTGA 4 cut(s) 40, 146, 422, 1595
Hpy166II GTNNAC 8 cut(s) 997, 1222, 1414, 1576, 1812, 2067, 2086, 2505
Hpy188I TCNGA 8 cut(s) 381, 688, 712, 941, 1778, 1957, 2080, 2160
Hpy8I GTNNAC 8 cut(s) 997, 1222, 1414, 1576, 1812, 2067, 2086, 2505
Hpy99I CGWCG 1 cut(s) 2058
HpyAV CCTTC 3 cut(s) 1877, 2228, 2531
HpyCH4III ACNGT 8 cut(s) 89, 112, 785, 1113, 1309, 1423, 1809, 2246
HpyCH4IV ACGT 1 cut(s) 1814
HpyF10VI GCNNNNNNNGC 7 cut(s) 599, 1292, 1301, 1310, 1319, 1681, 2177
HpyF3I CTNAG 6 cut(s) 164, 1506, 1685, 1775, 2043, 2250
HpySE526I ACGT 1 cut(s) 1814
Hsp92II CATG 6 cut(s) 751, 898, 903, 1839, 2003, 2373
HspAI GCGC 1 cut(s) 1845
Kpn2I TCCGGA 1 cut(s) 2376
KroI GCCGGC 1 cut(s) 2310
KroNI GCCGGC 1 cut(s) 2312
Ksp22I TGATCA 1 cut(s) 750
LguI GCTCTTC 1 cut(s) 2300
LmnI GCTCC 5 cut(s) 226, 312, 832, 1292, 1877
Lsp1109I GCAGC 4 cut(s) 1028, 1135, 1529, 1834
LweI GCATC 3 cut(s) 493, 1150, 1662
MaeI CTAG 8 cut(s) 509, 603, 741, 959, 1319, 1928, 2036, 2508
MaeII ACGT 1 cut(s) 1814
MaeIII GTNAC 4 cut(s) 106, 1367, 1500, 2202
MbiI CCGCTC 1 cut(s) 1295
MfeI CAATTG 1 cut(s) 1287
MflI RGATCY 4 cut(s) 712, 916, 2038, 2373
MhlI GDGCHC 1 cut(s) 231
MlyI GAGTC 5 cut(s) 1002, 1241, 1765, 2261, 2510
MmeI TCCRAC 4 cut(s) 888, 1537, 1647, 1980
Mph1103I ATGCAT 2 cut(s) 433, 1841
MroI TCCGGA 1 cut(s) 2376
MroNI GCCGGC 1 cut(s) 2310
MroXI GAANNNNTTC 1 cut(s) 887
MseI TTAA 3 cut(s) 552, 1908, 2016
MslI CAYNNNNRTG 4 cut(s) 426, 951, 1902, 1998
MspA1I CMGCKG 1 cut(s) 575
MspI CCGG 5 cut(s) 527, 765, 1875, 2311, 2377
MspR9I CCNGG 6 cut(s) 408, 527, 766, 921, 1389, 1646
MunI CAATTG 1 cut(s) 1287
Mva1269I GAATGC 1 cut(s) 433
MvaI CCWGG 4 cut(s) 408, 921, 1389, 1646
MwoI GCNNNNNNNGC 7 cut(s) 599, 1292, 1301, 1310, 1319, 1681, 2177
NaeI GCCGGC 1 cut(s) 2312
NciI CCSGG 2 cut(s) 527, 766
NdeI CATATG 1 cut(s) 220
NgoMIV GCCGGC 1 cut(s) 2310
NlaIII CATG 6 cut(s) 751, 898, 903, 1839, 2003, 2373
NlaIV GGNNCC 6 cut(s) 308, 405, 828, 918, 1128, 2375
NmeAIII GCCGAG 1 cut(s) 2104
NmuCI GTSAC 3 cut(s) 106, 1367, 2202
NsiI ATGCAT 2 cut(s) 433, 1841
NspI RCATGY 2 cut(s) 898, 1839
NspV TTCGAA 2 cut(s) 47, 1262
PaeI GCATGC 1 cut(s) 1839
PaeR7I CTCGAG 1 cut(s) 1851
PceI AGGCCT 1 cut(s) 1644
PciI ACATGT 1 cut(s) 894
PciSI GCTCTTC 1 cut(s) 2300
PctI GAATGC 1 cut(s) 433
PdiI GCCGGC 1 cut(s) 2312
PdmI GAANNNNTTC 1 cut(s) 887
PfoI TCCNGGA 1 cut(s) 525
PkrI GCNGC 4 cut(s) 1018, 1150, 1519, 1849
PleI GAGTC 5 cut(s) 1001, 1240, 1765, 2260, 2509
PpsI GAGTC 5 cut(s) 1001, 1240, 1765, 2260, 2509
PpuMI RGGWCCY 1 cut(s) 1127
PscI ACATGT 1 cut(s) 894
Psp124BI GAGCTC 1 cut(s) 231
Psp5II RGGWCCY 1 cut(s) 1127
Psp6I CCWGG 4 cut(s) 406, 919, 1387, 1644
PspFI CCCAGC 1 cut(s) 1635
PspGI CCWGG 4 cut(s) 406, 919, 1387, 1644
PspN4I GGNNCC 6 cut(s) 308, 405, 828, 918, 1128, 2375
PspPI GGNCC 4 cut(s) 1127, 1620, 1979, 1990
PspPPI RGGWCCY 1 cut(s) 1127
PspXI VCTCGAGB 1 cut(s) 1851
PstI CTGCAG 4 cut(s) 441, 1018, 1153, 2014
PstNI CAGNNNCTG 1 cut(s) 1157
PsuI RGATCY 4 cut(s) 712, 916, 2038, 2373
PvuII CAGCTG 1 cut(s) 575
RsaI GTAC 2 cut(s) 94, 2008
RsaNI GTAC 2 cut(s) 93, 2007
RseI CAYNNNNRTG 4 cut(s) 426, 951, 1902, 1998
SacI GAGCTC 1 cut(s) 231
SapI GCTCTTC 1 cut(s) 2300
SaqAI TTAA 3 cut(s) 552, 1908, 2016
SatI GCNGC 4 cut(s) 1017, 1149, 1518, 1848
Sau96I GGNCC 4 cut(s) 1127, 1620, 1979, 1990
SbfI CCTGCAGG 1 cut(s) 441
ScaI AGTACT 1 cut(s) 2008
SchI GAGTC 5 cut(s) 1002, 1241, 1765, 2261, 2510
ScrFI CCNGG 6 cut(s) 408, 527, 766, 921, 1389, 1646
SdaI CCTGCAGG 1 cut(s) 441
SduI GDGCHC 1 cut(s) 231
SfaNI GCATC 3 cut(s) 493, 1150, 1662
SfcI CTRYAG 6 cut(s) 437, 576, 1014, 1149, 2010, 2532
Sfr274I CTCGAG 1 cut(s) 1851
SfuI TTCGAA 2 cut(s) 47, 1262
SinI GGWCC 4 cut(s) 1127, 1620, 1979, 1990
SlaI CTCGAG 1 cut(s) 1851
SmiMI CAYNNNNRTG 4 cut(s) 426, 951, 1902, 1998
SmlI CTYRAG 1 cut(s) 1851
SmoI CTYRAG 1 cut(s) 1851
SpeI ACTAGT 2 cut(s) 508, 2507
SphI GCATGC 1 cut(s) 1839
Sse8387I CCTGCAGG 1 cut(s) 441
SseBI AGGCCT 1 cut(s) 1644
SsiI CCGC 3 cut(s) 1295, 1492, 1618
SspI AATATT 1 cut(s) 793
SspMI CTAG 8 cut(s) 509, 603, 741, 959, 1319, 1928, 2036, 2508
SstI GAGCTC 1 cut(s) 231
StuI AGGCCT 1 cut(s) 1644
StyD4I CCNGG 6 cut(s) 406, 525, 764, 919, 1387, 1644
StyI CCWWGG 2 cut(s) 277, 1431
TaaI ACNGT 8 cut(s) 89, 112, 785, 1113, 1309, 1423, 1809, 2246
TaiI ACGT 1 cut(s) 1817
TatI WGTACW 1 cut(s) 2006
Tru1I TTAA 3 cut(s) 552, 1908, 2016
Tru9I TTAA 3 cut(s) 552, 1908, 2016
TscAI CASTG 7 cut(s) 109, 115, 178, 196, 828, 1116, 1426
TseFI GTSAC 3 cut(s) 106, 1367, 2202
TseI GCWGC 4 cut(s) 1016, 1148, 1517, 1847
Tsp45I GTSAC 3 cut(s) 106, 1367, 2202
TspGWI ACGGA 1 cut(s) 1676
TspRI CASTG 7 cut(s) 109, 115, 178, 196, 828, 1116, 1426
VpaK11BI GGWCC 4 cut(s) 1127, 1620, 1979, 1990
XapI RAATTY 4 cut(s) 97, 177, 395, 1652
XbaI TCTAGA 1 cut(s) 2035
XceI RCATGY 2 cut(s) 898, 1839
XcmI CCANNNNNNNNNTGG 1 cut(s) 1812
XhoI CTCGAG 1 cut(s) 1851
XmiI GTMKAC 4 cut(s) 996, 1575, 2085, 2504
XmnI GAANNNNTTC 1 cut(s) 887
XspI CTAG 8 cut(s) 509, 603, 741, 959, 1319, 1928, 2036, 2508
ZrmI AGTACT 1 cut(s) 2008
Zsp2I ATGCAT 2 cut(s) 433, 1841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.