MD10G1256700.v1.1

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011537
Physical Location & Seq
Forward (+)
35118809 .. 35121507
2699 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1256700.v1.1.491

Sequence Viewer

Length: 744 bp
ATGTCGTCAAACACTCATTCACCGTGCGCTGCGTGCAAGTTTCAACGGCGAAAATGCACGCAAGAATGCGTGTTCGCCCCCTATTTCCCACCAGACCAACCTCAAAAATTCGCAAACGTTCACACGGTTTACGGTGCGAGCAACGTCGGCAAGATCCTGAACGAACTCAATGTTGCATACCGTGAAGATGCGGTGACGTCATTGGCCTATGAGGCAGAGGCTCGTCTTCGGGACCCGGTGTATGGTTGTGTTGGTTTCATCTCATTTCTCCAGAACAGGCTTAAGCAAGTCCAGACTGATCTCTACAATGCCAAGAAAGATCTGTATGCCTATGTTGGCCCTCAGGCATTGATGATCCCTCTGTTGGGGGCTACCACTGGCCCTCCGCCCCAGCAGGGCTGCAGGGGAGGCCAGCTTTTGATTCGCGACCAACATCAGCAGCAATTGTTCGAGGCTCATCAGCAAGAGCTTGTCAGGTTCAATATCAATGGGATGGACGCCGCAGCGAGTTTGGTTAATGGAAATGGGTTTAGTCAGATTCCCGCAGCTGCTGCTAGTACCGGTGTGGTGTCTGGTTCATTGGGTTTGGGTGGAGCTTTTGATAATAACCATTATAACCAGATCCAACAAGAAGCAGCTGCACATCACCAGAATTCCCTAGACCATCAGCTTCAGGCTCGGCTGATGCTCCAACCACACCCGGCCCAGGAATCACAGCAGAAATCAGAAGGCGAGGAGAGTTGA

Protein Analysis

248

Amino Acids

27.03

Weight (kDa)

6.29

Isoelectric Point (pI)

44.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 8 - 105 7.9e-40 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 615
AatII GACGTC 1 cut(s) 200
AccII CGCG 1 cut(s) 426
AciI CCGC 4 cut(s) 191, 386, 501, 543
AclI AACGTT 1 cut(s) 117
AclWI GGATC 3 cut(s) 148, 349, 616
AcsI RAATTY 2 cut(s) 107, 652
AcuI CTGAAG 1 cut(s) 656
AcyI GRCGYC 2 cut(s) 197, 498
AfaI GTAC 1 cut(s) 559
AfiI CCNNNNNNNGG 5 cut(s) 242, 364, 365, 395, 706
AflII CTTAAG 1 cut(s) 281
AgeI ACCGGT 1 cut(s) 560
AgsI TTSAA 2 cut(s) 44, 481
AjnI CCWGG 1 cut(s) 705
AluBI AGCT 6 cut(s) 415, 469, 548, 596, 638, 670
AluI AGCT 6 cut(s) 415, 469, 548, 596, 638, 670
AlwI GGATC 3 cut(s) 148, 349, 616
AlwNI CAGNNNCTG 1 cut(s) 551
AoxI GGCC 5 cut(s) 204, 337, 379, 409, 702
ApeKI GCWGC 9 cut(s) 29, 399, 439, 503, 545, 548, 551, 635, 638
ApoI RAATTY 2 cut(s) 107, 652
AsiGI ACCGGT 1 cut(s) 560
AspLEI GCGC 1 cut(s) 29
AspS9I GGNCC 4 cut(s) 232, 338, 380, 703
AsuC2I CCSGG 2 cut(s) 236, 701
AsuHPI GGTGA 3 cut(s) 12, 205, 638
AvaII GGWCC 1 cut(s) 232
AxyI CCTNAGG 1 cut(s) 342
BbsI GAAGAC 1 cut(s) 218
BbvI GCAGC 9 cut(s) 16, 386, 451, 515, 535, 538, 557, 625, 647
BccI CCATC 2 cut(s) 487, 672
BceAI ACGGC 1 cut(s) 62
BciT130I CCWGG 1 cut(s) 707
BcnI CCSGG 2 cut(s) 236, 701
BfaI CTAG 2 cut(s) 555, 659
BfmI CTRYAG 1 cut(s) 400
BfrI CTTAAG 1 cut(s) 281
BglI GCCNNNNNGGC 1 cut(s) 212
BglII AGATCT 1 cut(s) 319
Bme1390I CCNGG 3 cut(s) 236, 701, 707
Bme18I GGWCC 1 cut(s) 232
BmgT120I GGNCC 4 cut(s) 232, 338, 380, 703
BmiI GGNNCC 2 cut(s) 233, 234
BmrFI CCNGG 3 cut(s) 236, 701, 707
BmsI GCATC 2 cut(s) 178, 675
BpiI GAAGAC 1 cut(s) 218
BpmI CTGGAG 1 cut(s) 254
BpuMI CCSGG 2 cut(s) 236, 701
BsaHI GRCGYC 2 cut(s) 197, 498
BsaJI CCNNGG 1 cut(s) 705
BsaWI WCCGGW 1 cut(s) 560
BsaXI ACNNNNNCTCC 2 cut(s) 367, 397
Bsc4I CCNNNNNNNGG 5 cut(s) 242, 364, 365, 395, 706
Bse118I RCCGGY 1 cut(s) 560
Bse1I ACTGG 1 cut(s) 382
Bse21I CCTNAGG 1 cut(s) 342
BseBI CCWGG 1 cut(s) 707
BseDI CCNNGG 1 cut(s) 705
BseGI GGATG 1 cut(s) 498
BseLI CCNNNNNNNGG 5 cut(s) 242, 364, 365, 395, 706
BseMII CTCAG 1 cut(s) 356
BseNI ACTGG 1 cut(s) 382
BseXI GCAGC 9 cut(s) 16, 386, 451, 515, 535, 538, 557, 625, 647
BseYI CCCAGC 1 cut(s) 390
BsgI GTGCAG 1 cut(s) 624
Bsh1236I CGCG 1 cut(s) 426
BshFI GGCC 5 cut(s) 206, 339, 381, 411, 704
BshTI ACCGGT 1 cut(s) 560
BsiSI CCGG 3 cut(s) 236, 561, 701
BslFI GGGAC 1 cut(s) 245
BslI CCNNNNNNNGG 5 cut(s) 242, 364, 365, 395, 706
BsmFI GGGAC 1 cut(s) 245
BsmI GAATGC 1 cut(s) 71
BsnI GGCC 5 cut(s) 206, 339, 381, 411, 704
Bsp143I GATC 5 cut(s) 153, 298, 319, 354, 621
Bsp68I TCGCGA 1 cut(s) 426
BspACI CCGC 4 cut(s) 191, 386, 501, 543
BspANI GGCC 5 cut(s) 206, 339, 381, 411, 704
BspCNI CTCAG 1 cut(s) 355
BspFNI CGCG 1 cut(s) 426
BspLI GGNNCC 2 cut(s) 233, 234
BspMAI CTGCAG 1 cut(s) 404
BspPI GGATC 3 cut(s) 148, 349, 616
BspTI CTTAAG 1 cut(s) 281
BsrFI RCCGGY 1 cut(s) 560
BsrI ACTGG 1 cut(s) 382
BssAI RCCGGY 1 cut(s) 560
BssECI CCNNGG 1 cut(s) 705
BssMI GATC 5 cut(s) 153, 298, 319, 354, 621
BssNI GRCGYC 2 cut(s) 197, 498
Bst2UI CCWGG 1 cut(s) 707
Bst4CI ACNGT 4 cut(s) 24, 127, 134, 182
BstACI GRCGYC 2 cut(s) 197, 498
BstAFI CTTAAG 1 cut(s) 281
BstAPI GCANNNNNTGC 1 cut(s) 551
BstC8I GCNNGC 4 cut(s) 34, 59, 139, 413
BstDEI CTNAG 1 cut(s) 342
BstF5I GGATG 1 cut(s) 498
BstFNI CGCG 1 cut(s) 426
BstHHI GCGC 1 cut(s) 29
BstKTI GATC 5 cut(s) 156, 301, 322, 357, 624
BstMBI GATC 5 cut(s) 153, 298, 319, 354, 621
BstMWI GCNNNNNNNGC 5 cut(s) 33, 147, 212, 408, 551
BstNI CCWGG 1 cut(s) 707
BstSCI CCNGG 3 cut(s) 234, 699, 705
BstSFI CTRYAG 1 cut(s) 400
BstUI CGCG 1 cut(s) 426
BstV1I GCAGC 9 cut(s) 16, 386, 451, 515, 535, 538, 557, 625, 647
BstV2I GAAGAC 1 cut(s) 218
BstX2I RGATCY 3 cut(s) 153, 319, 621
BstYI RGATCY 3 cut(s) 153, 319, 621
Bsu36I CCTNAGG 1 cut(s) 342
BsuRI GGCC 5 cut(s) 206, 339, 381, 411, 704
BtsCI GGATG 1 cut(s) 498
BtsIMutI CAGTG 1 cut(s) 375
BtuMI TCGCGA 1 cut(s) 426
Cac8I GCNNGC 4 cut(s) 34, 59, 139, 413
CaiI CAGNNNCTG 1 cut(s) 551
CfoI GCGC 1 cut(s) 29
Cfr10I RCCGGY 1 cut(s) 560
Cfr13I GGNCC 4 cut(s) 232, 338, 380, 703
CseI GACGC 1 cut(s) 506
Csp6I GTAC 1 cut(s) 558
CspAI ACCGGT 1 cut(s) 560
CviQI GTAC 1 cut(s) 558
DdeI CTNAG 1 cut(s) 342
DpnI GATC 5 cut(s) 155, 300, 321, 356, 623
DpnII GATC 5 cut(s) 153, 298, 319, 354, 621
EciI GGCGGA 1 cut(s) 375
Eco47I GGWCC 1 cut(s) 232
Eco57I CTGAAG 1 cut(s) 656
Eco81I CCTNAGG 1 cut(s) 342
EcoO109I RGGNCCY 1 cut(s) 232
EcoRI GAATTC 1 cut(s) 652
EcoRII CCWGG 1 cut(s) 705
FaiI YATR 6 cut(s) 178, 210, 243, 327, 333, 615
FaqI GGGAC 1 cut(s) 245
FauI CCCGC 1 cut(s) 550
FokI GGATG 1 cut(s) 505
FspBI CTAG 2 cut(s) 555, 659
GlaI GCGC 1 cut(s) 28
GsaI CCCAGC 1 cut(s) 394
GsuI CTGGAG 1 cut(s) 254
HaeIII GGCC 5 cut(s) 206, 339, 381, 411, 704
HapII CCGG 3 cut(s) 236, 561, 701
HgaI GACGC 1 cut(s) 506
HhaI GCGC 1 cut(s) 29
Hin1I GRCGYC 2 cut(s) 197, 498
Hin6I GCGC 1 cut(s) 27
HinP1I GCGC 1 cut(s) 27
HinfI GANTC 3 cut(s) 421, 538, 710
HpaII CCGG 3 cut(s) 236, 561, 701
HphI GGTGA 3 cut(s) 12, 205, 638
Hpy166II GTNNAC 2 cut(s) 121, 130
Hpy188I TCNGA 2 cut(s) 537, 727
Hpy188III TCNNGA 5 cut(s) 157, 230, 271, 292, 425
Hpy8I GTNNAC 2 cut(s) 121, 130
Hpy99I CGWCG 1 cut(s) 149
HpyAV CCTTC 1 cut(s) 722
HpyCH4III ACNGT 4 cut(s) 24, 127, 134, 182
HpyCH4IV ACGT 3 cut(s) 117, 144, 197
HpyCH4V TGCA 5 cut(s) 36, 57, 176, 402, 641
HpyF10VI GCNNNNNNNGC 5 cut(s) 33, 147, 212, 408, 551
HpyF3I CTNAG 1 cut(s) 342
HpySE526I ACGT 3 cut(s) 117, 144, 197
Hsp92I GRCGYC 2 cut(s) 197, 498
HspAI GCGC 1 cut(s) 27
KflI GGGWCCC 1 cut(s) 232
Kzo9I GATC 5 cut(s) 153, 298, 319, 354, 621
LmnI GCTCC 2 cut(s) 593, 693
Lsp1109I GCAGC 9 cut(s) 16, 386, 451, 515, 535, 538, 557, 625, 647
LweI GCATC 2 cut(s) 178, 675
MaeI CTAG 2 cut(s) 555, 659
MaeII ACGT 3 cut(s) 117, 144, 197
MaeIII GTNAC 1 cut(s) 193
MalI GATC 5 cut(s) 155, 300, 321, 356, 623
MboI GATC 5 cut(s) 153, 298, 319, 354, 621
MboII GAAGA 2 cut(s) 197, 218
MfeI CAATTG 1 cut(s) 443
MflI RGATCY 3 cut(s) 153, 319, 621
MluCI AATT 3 cut(s) 107, 443, 652
MmeI TCCRAC 2 cut(s) 649, 715
MnlI CCTC 9 cut(s) 111, 205, 211, 351, 369, 393, 401, 445, 727
MseI TTAA 2 cut(s) 282, 516
MspA1I CMGCKG 2 cut(s) 548, 638
MspCI CTTAAG 1 cut(s) 281
MspI CCGG 3 cut(s) 236, 561, 701
MspR9I CCNGG 3 cut(s) 236, 701, 707
MunI CAATTG 1 cut(s) 443
Mva1269I GAATGC 1 cut(s) 71
MvaI CCWGG 1 cut(s) 707
MvnI CGCG 1 cut(s) 426
MwoI GCNNNNNNNGC 5 cut(s) 33, 147, 212, 408, 551
NciI CCSGG 2 cut(s) 236, 701
NdeII GATC 5 cut(s) 153, 298, 319, 354, 621
NlaIV GGNNCC 2 cut(s) 233, 234
NmeAIII GCCGAG 1 cut(s) 658
NmuCI GTSAC 1 cut(s) 193
NruI TCGCGA 1 cut(s) 426
PctI GAATGC 1 cut(s) 71
PfeI GAWTC 3 cut(s) 421, 538, 710
PinAI ACCGGT 1 cut(s) 560
PpuMI RGGWCCY 1 cut(s) 232
PsiI TTATAA 1 cut(s) 615
Psp1406I AACGTT 1 cut(s) 117
Psp5II RGGWCCY 1 cut(s) 232
Psp6I CCWGG 1 cut(s) 705
PspFI CCCAGC 1 cut(s) 390
PspGI CCWGG 1 cut(s) 705
PspN4I GGNNCC 2 cut(s) 233, 234
PspPI GGNCC 4 cut(s) 232, 338, 380, 703
PspPPI RGGWCCY 1 cut(s) 232
PstI CTGCAG 1 cut(s) 404
PstNI CAGNNNCTG 1 cut(s) 551
PsuI RGATCY 3 cut(s) 153, 319, 621
PvuII CAGCTG 2 cut(s) 548, 638
RruI TCGCGA 1 cut(s) 426
RsaI GTAC 1 cut(s) 559
RsaNI GTAC 1 cut(s) 558
SaqAI TTAA 2 cut(s) 282, 516
Sau3AI GATC 5 cut(s) 153, 298, 319, 354, 621
Sau96I GGNCC 4 cut(s) 232, 338, 380, 703
ScrFI CCNGG 3 cut(s) 236, 701, 707
SfaNI GCATC 2 cut(s) 178, 675
SfcI CTRYAG 1 cut(s) 400
SinI GGWCC 1 cut(s) 232
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
Sse9I AATT 3 cut(s) 107, 443, 652
SsiI CCGC 4 cut(s) 191, 386, 501, 543
SspMI CTAG 2 cut(s) 555, 659
StyD4I CCNGG 3 cut(s) 234, 699, 705
TaaI ACNGT 4 cut(s) 24, 127, 134, 182
TaiI ACGT 3 cut(s) 120, 147, 200
TaqI TCGA 1 cut(s) 450
TasI AATT 3 cut(s) 107, 443, 652
TauI GCSGC 1 cut(s) 503
TfiI GAWTC 3 cut(s) 421, 538, 710
Tru1I TTAA 2 cut(s) 282, 516
Tru9I TTAA 2 cut(s) 282, 516
TscAI CASTG 1 cut(s) 382
TseFI GTSAC 1 cut(s) 193
TseI GCWGC 9 cut(s) 29, 399, 439, 503, 545, 548, 551, 635, 638
Tsp45I GTSAC 1 cut(s) 193
TspDTI ATGAA 2 cut(s) 247, 567
TspRI CASTG 1 cut(s) 382
Vha464I CTTAAG 1 cut(s) 281
VpaK11BI GGWCC 1 cut(s) 232
XapI RAATTY 2 cut(s) 107, 652
XspI CTAG 2 cut(s) 555, 659
ZraI GACGTC 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.