Rroxscaffold_1G00061510

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0011537
Physical Location & Seq
Forward (+)
83626155 .. 83626937
783 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00061510.1

Sequence Viewer

Length: 783 bp
ATGTCGTCCAACACTCACTCTCCGTGCGCCGCGTGCAAGTTTCTCCGGCGGAAATGCACGCAGGAGTGCGTTTTCGCGCCGTACTTCCCGCCGGACCAGCCCCAGAAATTCGCCAACGTCCACCGTGTGTACGGCGCAAGCAACGTGGCCAAGATCCTCAACGAGCTCAACGCCGCCCACCGTGAGGACGCCGTGACGTCACTGGCCTATGAGGCCGAGGCTCGCCTCCGGGACCCCGTGTACGGCTGCGTGGGGCTCATCTCCATCCTCCAGAACAGGCTCAAGCAGCTCCAGACGGATCTCTACAATGCCAAGAAGGATCTCTCGACCTACATTGGCCCTCAAGCGCTGATGATTCCAGTGTTCCATCCGCAGCAGCCGGAGATGAATGGGGGTGGCGCAATGTCTGTCCCCATGTTGGGAGTCCCAACTGGCCCGCAGCAGGGCGGACTTGCTGGGGGACAGTTGGTGATTAGGGATCAGCAGCAGCAGCAGCAGATGTTTGAGGCTCAGCAGCAGGAGCTGGTGAGGTTCAATGTGAATGGGATGGACAATGGAAATGGGTTTAATCAGATGGCTGGAGGAGGCGGTAGTGGTGGTGGAGGTAGTAATGGTGTGGTGTCAGGTCTGGGAGGTTTTGATAACAATGCTTTCAATAACCAGATGCAAGCTGCGGCCGCTGCACATCATCATCAGCATTCTCTTGACTATCAGCTTCAGGCTCAGCTCATGCTCCAACCACAGCAGCAATCGCAGCCGAAATCAGAGGGTGAGGAGAGTTGA

Protein Analysis

260

Amino Acids

28.07

Weight (kDa)

6.22

Isoelectric Point (pI)

54.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 8 - 105 3.7e-41 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 200
AccII CGCG 2 cut(s) 32, 77
AclWI GGATC 4 cut(s) 148, 306, 327, 486
AcoI YGGCCR 2 cut(s) 147, 675
AcsI RAATTY 1 cut(s) 107
AcuI CTGAAG 1 cut(s) 701
AcyI GRCGYC 2 cut(s) 189, 197
AdeI CACNNNGTG 1 cut(s) 127
AfaI GTAC 3 cut(s) 83, 131, 242
AfeI AGCGCT 1 cut(s) 348
AfiI CCNNNNNNNGG 6 cut(s) 184, 242, 418, 419, 442, 443
AgsI TTSAA 2 cut(s) 535, 655
AluBI AGCT 6 cut(s) 166, 289, 523, 671, 715, 727
AluI AGCT 6 cut(s) 166, 289, 523, 671, 715, 727
Alw21I GWGCWC 1 cut(s) 168
AlwI GGATC 4 cut(s) 148, 306, 327, 486
AlwNI CAGNNNCTG 1 cut(s) 523
Aor51HI AGCGCT 1 cut(s) 348
AoxI GGCC 6 cut(s) 147, 204, 213, 337, 433, 675
ApoI RAATTY 1 cut(s) 107
AspLEI GCGC 5 cut(s) 29, 79, 137, 349, 401
AspS9I GGNCC 4 cut(s) 94, 232, 338, 434
AsuC2I CCSGG 1 cut(s) 230
AsuHPI GGTGA 3 cut(s) 481, 538, 782
AvaII GGWCC 2 cut(s) 94, 232
BalI TGGCCA 1 cut(s) 149
BanII GRGCYC 2 cut(s) 168, 258
Bbv12I GWGCWC 1 cut(s) 168
BccI CCATC 4 cut(s) 272, 375, 541, 568
BceAI ACGGC 4 cut(s) 64, 148, 176, 259
BcnI CCSGG 1 cut(s) 230
BfoI RGCGCY 1 cut(s) 350
BglI GCCNNNNNGGC 1 cut(s) 212
BlpI GCTNAGC 2 cut(s) 510, 723
Bme1390I CCNGG 1 cut(s) 230
Bme18I GGWCC 2 cut(s) 94, 232
BmgT120I GGNCC 4 cut(s) 94, 232, 338, 434
BmiI GGNNCC 2 cut(s) 233, 234
BmrFI CCNGG 1 cut(s) 230
BmsI GCATC 1 cut(s) 654
BpmI CTGGAG 3 cut(s) 254, 275, 600
Bpu1102I GCTNAGC 2 cut(s) 510, 723
BpuEI CTTGAG 2 cut(s) 266, 327
BpuMI CCSGG 1 cut(s) 230
BsaHI GRCGYC 2 cut(s) 189, 197
BsaJI CCNNGG 1 cut(s) 216
BsaXI ACNNNNNCTCC 1 cut(s) 34
Bsc4I CCNNNNNNNGG 6 cut(s) 184, 242, 418, 419, 442, 443
Bse1I ACTGG 3 cut(s) 207, 359, 436
Bse3DI GCAATG 1 cut(s) 408
BseDI CCNNGG 1 cut(s) 216
BseGI GGATG 3 cut(s) 264, 367, 552
BseLI CCNNNNNNNGG 6 cut(s) 184, 242, 418, 419, 442, 443
BseMI GCAATG 1 cut(s) 408
BseMII CTCAG 2 cut(s) 524, 737
BseNI ACTGG 3 cut(s) 207, 359, 436
BseRI GAGGAG 1 cut(s) 597
BseX3I CGGCCG 1 cut(s) 675
BseYI CCCAGC 1 cut(s) 455
BsgI GTGCAG 1 cut(s) 666
Bsh1236I CGCG 2 cut(s) 32, 77
Bsh1285I CGRYCG 1 cut(s) 678
BshFI GGCC 6 cut(s) 149, 206, 215, 339, 435, 677
BsiEI CGRYCG 1 cut(s) 678
BsiHKAI GWGCWC 1 cut(s) 168
BsiSI CCGG 4 cut(s) 46, 92, 229, 380
BslFI GGGAC 4 cut(s) 245, 395, 410, 474
BslI CCNNNNNNNGG 6 cut(s) 184, 242, 418, 419, 442, 443
BsmFI GGGAC 4 cut(s) 245, 395, 410, 474
BsmI GAATGC 1 cut(s) 697
BsnI GGCC 6 cut(s) 149, 206, 215, 339, 435, 677
Bsp1286I GDGCHC 2 cut(s) 168, 258
Bsp143I GATC 4 cut(s) 153, 298, 319, 478
Bsp1720I GCTNAGC 2 cut(s) 510, 723
BspANI GGCC 6 cut(s) 149, 206, 215, 339, 435, 677
BspCNI CTCAG 2 cut(s) 523, 736
BspFNI CGCG 2 cut(s) 32, 77
BspLI GGNNCC 2 cut(s) 233, 234
BspPI GGATC 4 cut(s) 148, 306, 327, 486
BsrDI GCAATG 1 cut(s) 408
BsrI ACTGG 3 cut(s) 207, 359, 436
BssECI CCNNGG 1 cut(s) 216
BssMI GATC 4 cut(s) 153, 298, 319, 478
BssNI GRCGYC 2 cut(s) 189, 197
Bst4CI ACNGT 3 cut(s) 125, 182, 465
BstACI GRCGYC 2 cut(s) 189, 197
BstC8I GCNNGC 6 cut(s) 34, 59, 139, 223, 437, 669
BstDEI CTNAG 2 cut(s) 510, 723
BstF5I GGATG 3 cut(s) 264, 367, 552
BstFNI CGCG 2 cut(s) 32, 77
BstH2I RGCGCY 1 cut(s) 350
BstHHI GCGC 5 cut(s) 29, 79, 137, 349, 401
BstKTI GATC 4 cut(s) 156, 301, 322, 481
BstMBI GATC 4 cut(s) 153, 298, 319, 478
BstMCI CGRYCG 1 cut(s) 678
BstSCI CCNGG 1 cut(s) 228
BstUI CGCG 2 cut(s) 32, 77
BstX2I RGATCY 3 cut(s) 153, 298, 319
BstYI RGATCY 3 cut(s) 153, 298, 319
BstZI CGGCCG 1 cut(s) 675
BsuRI GGCC 6 cut(s) 149, 206, 215, 339, 435, 677
BtsCI GGATG 3 cut(s) 264, 367, 552
BtsIMutI CAGTG 2 cut(s) 200, 366
Cac8I GCNNGC 6 cut(s) 34, 59, 139, 223, 437, 669
CaiI CAGNNNCTG 1 cut(s) 523
CciNI GCGGCCGC 1 cut(s) 675
CfoI GCGC 5 cut(s) 29, 79, 137, 349, 401
Cfr13I GGNCC 4 cut(s) 94, 232, 338, 434
CseI GACGC 1 cut(s) 197
Csp6I GTAC 3 cut(s) 82, 130, 241
CspCI CAANNNNNGTGG 2 cut(s) 126, 161
CviAII CATG 2 cut(s) 415, 730
CviQI GTAC 3 cut(s) 82, 130, 241
DdeI CTNAG 2 cut(s) 510, 723
DpnI GATC 4 cut(s) 155, 300, 321, 480
DpnII GATC 4 cut(s) 153, 298, 319, 478
DraIII CACNNNGTG 1 cut(s) 127
EaeI YGGCCR 2 cut(s) 147, 675
EagI CGGCCG 1 cut(s) 675
EciI GGCGGA 2 cut(s) 64, 462
Ecl136II GAGCTC 1 cut(s) 166
EclXI CGGCCG 1 cut(s) 675
Eco24I GRGCYC 2 cut(s) 168, 258
Eco47I GGWCC 2 cut(s) 94, 232
Eco47III AGCGCT 1 cut(s) 348
Eco52I CGGCCG 1 cut(s) 675
Eco53kI GAGCTC 1 cut(s) 166
Eco57I CTGAAG 1 cut(s) 701
EcoICRI GAGCTC 1 cut(s) 166
EcoO109I RGGNCCY 1 cut(s) 232
EcoT38I GRGCYC 2 cut(s) 168, 258
FaeI CATG 2 cut(s) 418, 733
FaiI YATR 3 cut(s) 210, 416, 731
FaqI GGGAC 4 cut(s) 245, 395, 410, 474
FatI CATG 2 cut(s) 414, 729
FauI CCCGC 2 cut(s) 96, 444
FokI GGATG 3 cut(s) 251, 354, 559
FriOI GRGCYC 2 cut(s) 168, 258
GlaI GCGC 5 cut(s) 28, 78, 136, 348, 400
GsaI CCCAGC 1 cut(s) 459
GsuI CTGGAG 3 cut(s) 254, 275, 600
HaeII RGCGCY 1 cut(s) 350
HaeIII GGCC 6 cut(s) 149, 206, 215, 339, 435, 677
HapII CCGG 4 cut(s) 46, 92, 229, 380
HgaI GACGC 1 cut(s) 197
HhaI GCGC 5 cut(s) 29, 79, 137, 349, 401
Hin1I GRCGYC 2 cut(s) 189, 197
Hin1II CATG 2 cut(s) 418, 733
Hin6I GCGC 5 cut(s) 27, 77, 135, 347, 399
HinP1I GCGC 5 cut(s) 27, 77, 135, 347, 399
HinfI GANTC 2 cut(s) 355, 423
HpaII CCGG 4 cut(s) 46, 92, 229, 380
HphI GGTGA 3 cut(s) 481, 538, 782
Hpy166II GTNNAC 3 cut(s) 121, 130, 241
Hpy188I TCNGA 2 cut(s) 573, 766
Hpy188III TCNNGA 4 cut(s) 271, 292, 325, 704
Hpy8I GTNNAC 3 cut(s) 121, 130, 241
HpyAV CCTTC 1 cut(s) 310
HpyCH4III ACNGT 3 cut(s) 125, 182, 465
HpyCH4IV ACGT 3 cut(s) 117, 144, 197
HpyCH4V TGCA 4 cut(s) 36, 57, 667, 683
HpyF3I CTNAG 2 cut(s) 510, 723
HpySE526I ACGT 3 cut(s) 117, 144, 197
Hsp92I GRCGYC 2 cut(s) 189, 197
Hsp92II CATG 2 cut(s) 418, 733
HspAI GCGC 5 cut(s) 27, 77, 135, 347, 399
KflI GGGWCCC 1 cut(s) 232
Kzo9I GATC 4 cut(s) 153, 298, 319, 478
LmnI GCTCC 3 cut(s) 294, 520, 738
LweI GCATC 1 cut(s) 654
MaeII ACGT 3 cut(s) 117, 144, 197
MaeIII GTNAC 2 cut(s) 193, 198
MalI GATC 4 cut(s) 155, 300, 321, 480
MboI GATC 4 cut(s) 153, 298, 319, 478
MflI RGATCY 3 cut(s) 153, 298, 319
MhlI GDGCHC 2 cut(s) 168, 258
MlsI TGGCCA 1 cut(s) 149
MluCI AATT 1 cut(s) 107
MluNI TGGCCA 1 cut(s) 149
MlyI GAGTC 1 cut(s) 432
MmeI TCCRAC 2 cut(s) 33, 760
Mox20I TGGCCA 1 cut(s) 149
MscI TGGCCA 1 cut(s) 149
MseI TTAA 1 cut(s) 567
Msp20I TGGCCA 1 cut(s) 149
MspA1I CMGCKG 1 cut(s) 680
MspI CCGG 4 cut(s) 46, 92, 229, 380
MspR9I CCNGG 1 cut(s) 230
Mva1269I GAATGC 1 cut(s) 697
MvnI CGCG 2 cut(s) 32, 77
NciI CCSGG 1 cut(s) 230
NdeII GATC 4 cut(s) 153, 298, 319, 478
NlaIII CATG 2 cut(s) 418, 733
NlaIV GGNNCC 2 cut(s) 233, 234
NmeAIII GCCGAG 1 cut(s) 241
NmuCI GTSAC 2 cut(s) 193, 198
NotI GCGGCCGC 1 cut(s) 675
PctI GAATGC 1 cut(s) 697
PfeI GAWTC 1 cut(s) 355
PfoI TCCNGGA 1 cut(s) 228
PleI GAGTC 1 cut(s) 431
PpsI GAGTC 1 cut(s) 431
PpuMI RGGWCCY 1 cut(s) 232
Psp124BI GAGCTC 1 cut(s) 168
Psp5II RGGWCCY 1 cut(s) 232
PspFI CCCAGC 1 cut(s) 455
PspN4I GGNNCC 2 cut(s) 233, 234
PspPI GGNCC 4 cut(s) 94, 232, 338, 434
PspPPI RGGWCCY 1 cut(s) 232
PstNI CAGNNNCTG 1 cut(s) 523
PsuI RGATCY 3 cut(s) 153, 298, 319
RsaI GTAC 3 cut(s) 83, 131, 242
RsaNI GTAC 3 cut(s) 82, 130, 241
SacI GAGCTC 1 cut(s) 168
SaqAI TTAA 1 cut(s) 567
Sau3AI GATC 4 cut(s) 153, 298, 319, 478
Sau96I GGNCC 4 cut(s) 94, 232, 338, 434
SchI GAGTC 1 cut(s) 432
ScrFI CCNGG 1 cut(s) 230
SduI GDGCHC 2 cut(s) 168, 258
SfaNI GCATC 1 cut(s) 654
SfiI GGCCNNNNNGGCC 1 cut(s) 212
SinI GGWCC 2 cut(s) 94, 232
SmlI CTYRAG 2 cut(s) 281, 342
SmoI CTYRAG 2 cut(s) 281, 342
Sse9I AATT 1 cut(s) 107
SstI GAGCTC 1 cut(s) 168
StyD4I CCNGG 1 cut(s) 228
TaaI ACNGT 3 cut(s) 125, 182, 465
TaiI ACGT 3 cut(s) 120, 147, 200
TaqI TCGA 1 cut(s) 326
TasI AATT 1 cut(s) 107
TauI GCSGC 4 cut(s) 32, 176, 677, 680
TfiI GAWTC 1 cut(s) 355
Tru1I TTAA 1 cut(s) 567
Tru9I TTAA 1 cut(s) 567
TscAI CASTG 2 cut(s) 207, 366
TseFI GTSAC 2 cut(s) 193, 198
Tsp45I GTSAC 2 cut(s) 193, 198
TspDTI ATGAA 1 cut(s) 401
TspGWI ACGGA 2 cut(s) 12, 311
TspRI CASTG 2 cut(s) 207, 366
VpaK11BI GGWCC 2 cut(s) 94, 232
XapI RAATTY 1 cut(s) 107
ZraI GACGTC 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.