MD10G1263600.v1.1

Eukaryotic cytochrome b561

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011768
Physical Location & Seq
Forward (+)
35766413 .. 35768505
2093 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1263600.v1.1.491

Sequence Viewer

Length: 816 bp
ATGCAGACTTCCCTCAAGCTAGCATCTTTCACTATTTCTACATATTATCTTCTTCTTCTTCTTCTACCATTAGTTAGTTGCTCATTGCATGCAGATGTCAACCAACACAGCAGTCACGAGAACATAAAAGAAAATATTCACAAGATGAGTCCCCGGATGACGTTTAACGTTACAGTTCATGGGCTCCTCTCATGGTTTTCAATGGGGTTTTTGATGCCCCTTGGAATACTTGTAATTAGAATGTCTGCTAGAGAGGAACGTGGGGCAACAAGAGCCAAAGTTCTCTTCTATCTCCATATTGTTTTGCAGGTGCTGTCGGTGCTTCTTGCCACCGCTGGTGCTGCGATGTCCCTAAGAAACTTTGAGAACTCCTTCAACAACAACCACCAAAGATTAGGTCTAGCACTGTATGCTGCCATATGGATACAAACCCTAATAGGATTTTTCAGGCCTCGCAGGGGCAAGAAAGAAAGAAGTGTATGGTACTTGGCACATTGGATGCTTGGGACAGTGATATCCTTGGTAGGGATAATCAACATCTACACTGGATTAGAAGCCTACCATAAGAGAACACAGAGAAGCTCAGGGCTTTGGACTGTTCTTTTCACAGCTGAGGTTTCCTTCATTGCTGCGTATTATCTCTTCCAAGACAAAAGAGATTATATACAAAAACAAGGAGTGCTTTTAGGCACAACCAGGCAGCAACAGCAAGAAGATGAAGAAATTGCTCAAAGGCAGCACCAAAATCAAAAGGAGATGTTGCCAGCTCAGCAGCCTAGTGGCAAAGTCAATGCACTCAAGAACTTGTTTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561

Protein Analysis

272

Amino Acids

31.04

Weight (kDa)

9.84

Isoelectric Point (pI)

51.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 59 - 185 3.5e-10 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011768)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18260
fragaria_vesca FvH4_3g05770 FvH4_3g05770
malus_domestica MD05G1285600.v1.1 MD10G1263600.v1.1
prunus_persica Prupe.4G079100_v2.0.a1
pyrus_communis pycom05g26460 pycom10g21980
rosa_chinensis RchiOBHm_Chr5g0013761
rosa_laevigata RLG00000032044
rosa_multiflora Rmu_co8478607.1_g000001 Rmu_ssc0000256.1_g000002
rosa_roxburghii Rroxscaffold_1G00062880
rosa_rugosa Rorug05G0012700
rosa_samantha Rh5AG107000 Rh5BG103100 Rh5CG114900 Rh5DG101800
rosa_wichuraiana Rw5G009330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 298
Acc36I ACCTGC 1 cut(s) 298
AciI CCGC 1 cut(s) 333
AclI AACGTT 1 cut(s) 168
AfaI GTAC 1 cut(s) 485
AfiI CCNNNNNNNGG 2 cut(s) 458, 525
AgsI TTSAA 2 cut(s) 201, 376
AjnI CCWGG 1 cut(s) 695
AjuI GAANNNNNNNTTGG 2 cut(s) 269, 301
AluBI AGCT 4 cut(s) 19, 582, 611, 767
AluI AGCT 4 cut(s) 19, 582, 611, 767
AlwNI CAGNNNCTG 1 cut(s) 313
AoxI GGCC 1 cut(s) 449
ApeKI GCWGC 6 cut(s) 341, 413, 629, 700, 736, 772
ArsI GACNNNNNNTTYG 2 cut(s) 382, 414
Asp700I GAANNNNTTC 2 cut(s) 135, 371
AsuC2I CCSGG 1 cut(s) 154
AsuNHI GCTAGC 1 cut(s) 19
BanII GRGCYC 1 cut(s) 186
BarI GAAGNNNNNNTAC 2 cut(s) 626, 658
BauI CACGAG 1 cut(s) 116
BbvCI CCTCAGC 1 cut(s) 612
BbvI GCAGC 6 cut(s) 328, 400, 616, 712, 748, 784
BciT130I CCWGG 1 cut(s) 697
BciVI GTATCC 1 cut(s) 417
BcnI CCSGG 1 cut(s) 154
BfaI CTAG 4 cut(s) 20, 249, 401, 777
BfuAI ACCTGC 1 cut(s) 298
BfuI GTATCC 1 cut(s) 417
BisI GCNGC 6 cut(s) 342, 414, 630, 701, 737, 773
BlpI GCTNAGC 1 cut(s) 768
BlsI GCNGC 6 cut(s) 343, 415, 631, 702, 738, 774
Bme1390I CCNGG 2 cut(s) 154, 697
BmiI GGNNCC 1 cut(s) 185
BmrFI CCNGG 2 cut(s) 154, 697
BmsI GCATC 3 cut(s) 32, 204, 489
BmtI GCTAGC 1 cut(s) 23
Bpu10I CCTNAGC 2 cut(s) 583, 612
Bpu1102I GCTNAGC 1 cut(s) 768
BpuEI CTTGAG 1 cut(s) 782
BpuMI CCSGG 1 cut(s) 154
BsaJI CCNNGG 3 cut(s) 152, 220, 519
Bsc4I CCNNNNNNNGG 2 cut(s) 458, 525
Bse1I ACTGG 1 cut(s) 550
Bse3DI GCAATG 2 cut(s) 83, 624
BseBI CCWGG 1 cut(s) 697
BseDI CCNNGG 3 cut(s) 152, 220, 519
BseGI GGATG 2 cut(s) 162, 504
BseLI CCNNNNNNNGG 2 cut(s) 458, 525
BseMI GCAATG 2 cut(s) 83, 624
BseMII CTCAG 3 cut(s) 597, 603, 782
BseNI ACTGG 1 cut(s) 550
BseRI GAGGAG 1 cut(s) 176
BseXI GCAGC 6 cut(s) 328, 400, 616, 712, 748, 784
BshFI GGCC 1 cut(s) 451
BsiSI CCGG 1 cut(s) 154
BslFI GGGAC 3 cut(s) 135, 334, 520
BslI CCNNNNNNNGG 2 cut(s) 458, 525
BsmFI GGGAC 3 cut(s) 135, 334, 520
BsnI GGCC 1 cut(s) 451
Bsp1286I GDGCHC 1 cut(s) 186
Bsp1720I GCTNAGC 1 cut(s) 768
BspACI CCGC 1 cut(s) 333
BspANI GGCC 1 cut(s) 451
BspCNI CTCAG 3 cut(s) 596, 604, 781
BspLI GGNNCC 1 cut(s) 185
BspMI ACCTGC 1 cut(s) 298
BspOI GCTAGC 1 cut(s) 23
BsrDI GCAATG 2 cut(s) 83, 624
BsrI ACTGG 1 cut(s) 550
BssECI CCNNGG 3 cut(s) 152, 220, 519
BssSI CACGAG 1 cut(s) 116
BssT1I CCWWGG 2 cut(s) 220, 519
Bst2BI CACGAG 1 cut(s) 116
Bst2UI CCWGG 1 cut(s) 697
Bst4CI ACNGT 4 cut(s) 175, 408, 511, 598
Bst6I CTCTTC 2 cut(s) 290, 647
BstAPI GCANNNNNTGC 1 cut(s) 410
BstC8I GCNNGC 3 cut(s) 21, 90, 765
BstDEI CTNAG 4 cut(s) 353, 583, 612, 768
BstF5I GGATG 2 cut(s) 162, 504
BstMWI GCNNNNNNNGC 6 cut(s) 272, 319, 341, 410, 706, 769
BstNI CCWGG 1 cut(s) 697
BstNSI RCATGY 1 cut(s) 92
BstSCI CCNGG 2 cut(s) 152, 695
BstV1I GCAGC 6 cut(s) 328, 400, 616, 712, 748, 784
BsuI GTATCC 1 cut(s) 417
BsuRI GGCC 1 cut(s) 451
BtgZI GCGATG 1 cut(s) 359
BtsCI GGATG 2 cut(s) 162, 504
BtsIMutI CAGTG 3 cut(s) 404, 516, 543
BveI ACCTGC 1 cut(s) 298
Cac8I GCNNGC 3 cut(s) 21, 90, 765
CaiI CAGNNNCTG 1 cut(s) 313
Csp6I GTAC 1 cut(s) 484
CviAII CATG 3 cut(s) 89, 179, 192
CviQI GTAC 1 cut(s) 484
DdeI CTNAG 4 cut(s) 353, 583, 612, 768
Eam1104I CTCTTC 2 cut(s) 290, 647
EarI CTCTTC 2 cut(s) 290, 647
Eco130I CCWWGG 2 cut(s) 220, 519
Eco147I AGGCCT 1 cut(s) 451
Eco24I GRGCYC 1 cut(s) 186
Eco32I GATATC 1 cut(s) 516
EcoRII CCWGG 1 cut(s) 695
EcoRV GATATC 1 cut(s) 516
EcoT14I CCWWGG 2 cut(s) 220, 519
EcoT38I GRGCYC 1 cut(s) 186
ErhI CCWWGG 2 cut(s) 220, 519
FaeI CATG 3 cut(s) 92, 182, 195
FalI AAGNNNNNCTT 2 cut(s) 666, 698
FaqI GGGAC 3 cut(s) 135, 334, 520
FatI CATG 3 cut(s) 88, 178, 191
FauNDI CATATG 1 cut(s) 419
Fnu4HI GCNGC 6 cut(s) 342, 414, 630, 701, 737, 773
FokI GGATG 2 cut(s) 169, 511
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 6 cut(s) 342, 414, 630, 701, 737, 773
FspBI CTAG 4 cut(s) 20, 249, 401, 777
GluI GCNGC 6 cut(s) 342, 414, 630, 701, 737, 773
HaeIII GGCC 1 cut(s) 451
HapII CCGG 1 cut(s) 154
Hin1II CATG 3 cut(s) 92, 182, 195
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HinfI GANTC 1 cut(s) 148
HpaII CCGG 1 cut(s) 154
Hpy166II GTNNAC 1 cut(s) 100
Hpy188III TCNNGA 2 cut(s) 116, 799
Hpy8I GTNNAC 1 cut(s) 100
HpyAV CCTTC 2 cut(s) 382, 631
HpyCH4III ACNGT 4 cut(s) 175, 408, 511, 598
HpyCH4IV ACGT 3 cut(s) 161, 168, 259
HpyCH4V TGCA 5 cut(s) 4, 88, 92, 307, 794
HpyF10VI GCNNNNNNNGC 6 cut(s) 272, 319, 341, 410, 706, 769
HpyF3I CTNAG 4 cut(s) 353, 583, 612, 768
HpySE526I ACGT 3 cut(s) 161, 168, 259
Hsp92II CATG 3 cut(s) 92, 182, 195
LmnI GCTCC 1 cut(s) 189
Lsp1109I GCAGC 6 cut(s) 328, 400, 616, 712, 748, 784
LweI GCATC 3 cut(s) 32, 204, 489
MaeI CTAG 4 cut(s) 20, 249, 401, 777
MaeII ACGT 3 cut(s) 161, 168, 259
MaeIII GTNAC 2 cut(s) 113, 169
MboII GAAGA 9 cut(s) 41, 44, 47, 50, 53, 277, 634, 725, 731
MhlI GDGCHC 1 cut(s) 186
MluCI AATT 2 cut(s) 234, 723
MlyI GAGTC 1 cut(s) 157
MnlI CCTC 5 cut(s) 23, 197, 247, 462, 607
MroXI GAANNNNTTC 2 cut(s) 135, 371
MseI TTAA 1 cut(s) 165
MslI CAYNNNNRTG 1 cut(s) 93
MspA1I CMGCKG 2 cut(s) 335, 611
MspI CCGG 1 cut(s) 154
MspR9I CCNGG 2 cut(s) 154, 697
MvaI CCWGG 1 cut(s) 697
MwoI GCNNNNNNNGC 6 cut(s) 272, 319, 341, 410, 706, 769
NciI CCSGG 1 cut(s) 154
NdeI CATATG 1 cut(s) 419
NheI GCTAGC 1 cut(s) 19
NlaIII CATG 3 cut(s) 92, 182, 195
NlaIV GGNNCC 1 cut(s) 185
NmuCI GTSAC 1 cut(s) 113
NspI RCATGY 1 cut(s) 92
PaeI GCATGC 1 cut(s) 92
PaqCI CACCTGC 1 cut(s) 298
PceI AGGCCT 1 cut(s) 451
PdmI GAANNNNTTC 2 cut(s) 135, 371
PkrI GCNGC 6 cut(s) 343, 415, 631, 702, 738, 774
PleI GAGTC 1 cut(s) 156
PpsI GAGTC 1 cut(s) 156
Psp1406I AACGTT 1 cut(s) 168
Psp6I CCWGG 1 cut(s) 695
PspGI CCWGG 1 cut(s) 695
PspN4I GGNNCC 1 cut(s) 185
PstNI CAGNNNCTG 1 cut(s) 313
PvuII CAGCTG 1 cut(s) 611
RsaI GTAC 1 cut(s) 485
RsaNI GTAC 1 cut(s) 484
RseI CAYNNNNRTG 1 cut(s) 93
SaqAI TTAA 1 cut(s) 165
SatI GCNGC 6 cut(s) 342, 414, 630, 701, 737, 773
SchI GAGTC 1 cut(s) 157
ScrFI CCNGG 2 cut(s) 154, 697
SduI GDGCHC 1 cut(s) 186
SfaNI GCATC 3 cut(s) 32, 204, 489
SmiMI CAYNNNNRTG 1 cut(s) 93
SmlI CTYRAG 2 cut(s) 14, 797
SmoI CTYRAG 2 cut(s) 14, 797
SphI GCATGC 1 cut(s) 92
Sse9I AATT 2 cut(s) 234, 723
SseBI AGGCCT 1 cut(s) 451
SsiI CCGC 1 cut(s) 333
SspI AATATT 1 cut(s) 136
SspMI CTAG 4 cut(s) 20, 249, 401, 777
StuI AGGCCT 1 cut(s) 451
StyD4I CCNGG 2 cut(s) 152, 695
StyI CCWWGG 2 cut(s) 220, 519
TaaI ACNGT 4 cut(s) 175, 408, 511, 598
TaiI ACGT 3 cut(s) 164, 171, 262
TasI AATT 2 cut(s) 234, 723
Tru1I TTAA 1 cut(s) 165
Tru9I TTAA 1 cut(s) 165
TscAI CASTG 3 cut(s) 411, 516, 550
TseFI GTSAC 1 cut(s) 113
TseI GCWGC 6 cut(s) 341, 413, 629, 700, 736, 772
Tsp45I GTSAC 1 cut(s) 113
TspDTI ATGAA 3 cut(s) 167, 613, 732
TspRI CASTG 3 cut(s) 411, 516, 550
XceI RCATGY 1 cut(s) 92
XmnI GAANNNNTTC 2 cut(s) 135, 371
XspI CTAG 4 cut(s) 20, 249, 401, 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.