MD10G1275900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0012913
Physical Location & Seq
Reverse (-)
36714446 .. 36715453
1008 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1275900.v1.1.491

Sequence Viewer

Length: 537 bp
ATGAGAGCTATAGTCTCCGGGATGTGGAAGAGAGCAGCATTGCGCCGAAAGCTTCAAATTCTAAGGAGCCTTACCAAGACAAAATCCGTCAAGAGGAGCTCCATTATCATGGATGCTCTACTCCACATTTATATGCTGAAACTCAAGCTTGAAGCAGTCCAAATAGAGTACTTGCATCTCATGGCTATCAAAACAGATTACATAAACCTAATCAAACATCTTCAAGTCCCCAAGGAAGTGAAGGTAGAGAAGATTAAGGAAGGGTTTCTTGTGAGAGTGAAGTGCGAGAAAGCGAAGGACACTCTGGTTTCGGTTTTGGAGGCATTTGAAGAAATGGGTCTTAATGTTGCGCAGGCTAGAGTTTCATGCAACAATTATTTTTCCATGGAAGCCATTGCAGCTGTAGATGATGGCGAAAACAAAGACCAGATGGATGTGAGAGTCGTAACACAAGCAATACTAAGGGCCACCAAGATTAATCAAGATGGAAAGGAGACGCGTGAATGCATTGTAGTTCCCCAGGTTAATAACTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

179

Amino Acids

20.3

Weight (kDa)

9.51

Isoelectric Point (pI)

39.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-TF_ACT-like_plant PF22754 79 - 157 8.5e-23 Plant bHLH transcription factor, ACT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012913)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29270 AT1G29270
fragaria_vesca FvH4_3g07370 FvH4_3g07370
malus_domestica MD05G1295800.v1.1 MD10G1275900.v1.1
prunus_persica Prupe.4G066300_v2.0.a1
pyrus_communis pycom10g23060
rosa_chinensis RchiOBHm_Chr5g0011571
rosa_laevigata RLG00000031843
rosa_multiflora Rmu_sc0009001.1_g000010
rosa_roxburghii Rroxscaffold_1G00064920
rosa_rugosa Rorug04G0457600 Rorug04G0457700
rosa_samantha Rh5AG089400 Rh5CG098700 Rh5DG084700
rosa_wichuraiana Rw5G007770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 351
AccII CGCG 1 cut(s) 499
AcsI RAATTY 1 cut(s) 57
AfaI GTAC 1 cut(s) 170
AfiI CCNNNNNNNGG 2 cut(s) 24, 93
AflIII ACRYGT 1 cut(s) 497
AgsI TTSAA 4 cut(s) 56, 152, 224, 329
AjnI CCWGG 1 cut(s) 519
AluBI AGCT 5 cut(s) 8, 52, 99, 148, 401
AluI AGCT 5 cut(s) 8, 52, 99, 148, 401
Alw21I GWGCWC 1 cut(s) 101
Alw26I GTCTC 2 cut(s) 19, 488
AoxI GGCC 1 cut(s) 465
ApeKI GCWGC 2 cut(s) 35, 398
ApoI RAATTY 1 cut(s) 57
AseI ATTAAT 1 cut(s) 477
Asp700I GAANNNNTTC 1 cut(s) 264
AspLEI GCGC 2 cut(s) 45, 352
AspS9I GGNCC 1 cut(s) 465
AsuC2I CCSGG 1 cut(s) 19
BanII GRGCYC 1 cut(s) 101
Bbv12I GWGCWC 1 cut(s) 101
BbvI GCAGC 2 cut(s) 47, 410
BccI CCATC 3 cut(s) 404, 424, 479
BciT130I CCWGG 1 cut(s) 521
BcnI CCSGG 1 cut(s) 19
BcoDI GTCTC 2 cut(s) 19, 488
BfaI CTAG 1 cut(s) 357
BfmI CTRYAG 2 cut(s) 9, 402
BisI GCNGC 2 cut(s) 36, 399
BlsI GCNGC 2 cut(s) 37, 400
BmcAI AGTACT 1 cut(s) 170
Bme1390I CCNGG 2 cut(s) 19, 521
BmgT120I GGNCC 1 cut(s) 465
BmiI GGNNCC 1 cut(s) 68
BmrFI CCNGG 2 cut(s) 19, 521
BmsI GCATC 2 cut(s) 103, 184
BpuEI CTTGAG 1 cut(s) 128
BpuMI CCSGG 1 cut(s) 19
BsaJI CCNNGG 3 cut(s) 231, 384, 519
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 93
Bse3DI GCAATG 2 cut(s) 38, 393
BseBI CCWGG 1 cut(s) 521
BseDI CCNNGG 3 cut(s) 231, 384, 519
BseGI GGATG 3 cut(s) 27, 118, 439
BseLI CCNNNNNNNGG 2 cut(s) 24, 93
BseMI GCAATG 2 cut(s) 38, 393
BseRI GAGGAG 1 cut(s) 109
BseXI GCAGC 2 cut(s) 47, 410
Bsh1236I CGCG 1 cut(s) 499
BshFI GGCC 1 cut(s) 467
BsiHKAI GWGCWC 1 cut(s) 101
BsiSI CCGG 1 cut(s) 18
BslFI GGGAC 1 cut(s) 212
BslI CCNNNNNNNGG 2 cut(s) 24, 93
BsmAI GTCTC 2 cut(s) 19, 488
BsmBI CGTCTC 1 cut(s) 488
BsmFI GGGAC 1 cut(s) 212
BsmI GAATGC 1 cut(s) 509
BsnI GGCC 1 cut(s) 467
Bsp1286I GDGCHC 1 cut(s) 101
Bsp19I CCATGG 1 cut(s) 384
BspANI GGCC 1 cut(s) 467
BspFNI CGCG 1 cut(s) 499
BspLI GGNNCC 1 cut(s) 68
BsrDI GCAATG 2 cut(s) 38, 393
BssECI CCNNGG 3 cut(s) 231, 384, 519
BssT1I CCWWGG 2 cut(s) 231, 384
Bst2UI CCWGG 1 cut(s) 521
Bst6I CTCTTC 1 cut(s) 23
BstC8I GCNNGC 1 cut(s) 354
BstDEI CTNAG 2 cut(s) 62, 461
BstDSI CCRYGG 1 cut(s) 384
BstF5I GGATG 3 cut(s) 27, 118, 439
BstFNI CGCG 1 cut(s) 499
BstHHI GCGC 2 cut(s) 45, 352
BstMAI GTCTC 2 cut(s) 19, 488
BstMWI GCNNNNNNNGC 2 cut(s) 49, 398
BstNI CCWGG 1 cut(s) 521
BstSCI CCNGG 2 cut(s) 17, 519
BstSFI CTRYAG 2 cut(s) 9, 402
BstUI CGCG 1 cut(s) 499
BstV1I GCAGC 2 cut(s) 47, 410
BstXI CCANNNNNNTGG 1 cut(s) 109
BsuRI GGCC 1 cut(s) 467
BtgI CCRYGG 1 cut(s) 384
BtsCI GGATG 3 cut(s) 27, 118, 439
Cac8I GCNNGC 1 cut(s) 354
CfoI GCGC 2 cut(s) 45, 352
Cfr13I GGNCC 1 cut(s) 465
CseI GACGC 1 cut(s) 505
Csp6I GTAC 1 cut(s) 169
CviAII CATG 4 cut(s) 109, 181, 366, 385
CviQI GTAC 1 cut(s) 169
DdeI CTNAG 2 cut(s) 62, 461
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
Ecl136II GAGCTC 1 cut(s) 99
Eco130I CCWWGG 2 cut(s) 231, 384
Eco24I GRGCYC 1 cut(s) 101
Eco53kI GAGCTC 1 cut(s) 99
EcoICRI GAGCTC 1 cut(s) 99
EcoRII CCWGG 1 cut(s) 519
EcoT14I CCWWGG 2 cut(s) 231, 384
EcoT22I ATGCAT 1 cut(s) 509
EcoT38I GRGCYC 1 cut(s) 101
ErhI CCWWGG 2 cut(s) 231, 384
Esp3I CGTCTC 1 cut(s) 488
FaeI CATG 4 cut(s) 112, 184, 369, 388
FaiI YATR 8 cut(s) 11, 110, 132, 134, 182, 203, 367, 386
FalI AAGNNNNNCTT 2 cut(s) 252, 284
FaqI GGGAC 1 cut(s) 212
FatI CATG 4 cut(s) 108, 180, 365, 384
Fnu4HI GCNGC 2 cut(s) 36, 399
FokI GGATG 3 cut(s) 34, 125, 446
FriOI GRGCYC 1 cut(s) 101
Fsp4HI GCNGC 2 cut(s) 36, 399
FspBI CTAG 1 cut(s) 357
FspI TGCGCA 1 cut(s) 351
GlaI GCGC 2 cut(s) 44, 351
GluI GCNGC 2 cut(s) 36, 399
HaeIII GGCC 1 cut(s) 467
HapII CCGG 1 cut(s) 18
HgaI GACGC 1 cut(s) 505
HhaI GCGC 2 cut(s) 45, 352
Hin1II CATG 4 cut(s) 112, 184, 369, 388
Hin6I GCGC 2 cut(s) 43, 350
HinP1I GCGC 2 cut(s) 43, 350
HindIII AAGCTT 2 cut(s) 50, 146
HinfI GANTC 1 cut(s) 441
HpaII CCGG 1 cut(s) 18
Hpy188III TCNNGA 2 cut(s) 91, 482
HpyAV CCTTC 3 cut(s) 235, 254, 289
HpyCH4V TGCA 4 cut(s) 175, 369, 398, 507
HpyF10VI GCNNNNNNNGC 2 cut(s) 49, 398
HpyF3I CTNAG 2 cut(s) 62, 461
Hsp92II CATG 4 cut(s) 112, 184, 369, 388
HspAI GCGC 2 cut(s) 43, 350
LmnI GCTCC 3 cut(s) 66, 96, 104
LpnPI CCDG 6 cut(s) 31, 290, 338, 440, 506, 533
Lsp1109I GCAGC 2 cut(s) 47, 410
LweI GCATC 2 cut(s) 103, 184
MaeI CTAG 1 cut(s) 357
MaeIII GTNAC 1 cut(s) 445
MboII GAAGA 4 cut(s) 40, 212, 262, 341
MhlI GDGCHC 1 cut(s) 101
MluCI AATT 2 cut(s) 57, 373
MluI ACGCGT 1 cut(s) 497
MlyI GAGTC 1 cut(s) 450
MnlI CCTC 2 cut(s) 87, 313
Mph1103I ATGCAT 1 cut(s) 509
MroXI GAANNNNTTC 1 cut(s) 264
MseI TTAA 5 cut(s) 255, 342, 477, 525, 535
MslI CAYNNNNRTG 2 cut(s) 107, 131
MspA1I CMGCKG 1 cut(s) 401
MspI CCGG 1 cut(s) 18
MspR9I CCNGG 2 cut(s) 19, 521
Mva1269I GAATGC 1 cut(s) 509
MvaI CCWGG 1 cut(s) 521
MvnI CGCG 1 cut(s) 499
MwoI GCNNNNNNNGC 2 cut(s) 49, 398
NciI CCSGG 1 cut(s) 19
NcoI CCATGG 1 cut(s) 384
NlaIII CATG 4 cut(s) 112, 184, 369, 388
NlaIV GGNNCC 1 cut(s) 68
NsbI TGCGCA 1 cut(s) 351
NsiI ATGCAT 1 cut(s) 509
PctI GAATGC 1 cut(s) 509
PdmI GAANNNNTTC 1 cut(s) 264
PfoI TCCNGGA 1 cut(s) 17
PkrI GCNGC 2 cut(s) 37, 400
PleI GAGTC 1 cut(s) 449
PpsI GAGTC 1 cut(s) 449
PshBI ATTAAT 1 cut(s) 477
Psp124BI GAGCTC 1 cut(s) 101
Psp6I CCWGG 1 cut(s) 519
PspGI CCWGG 1 cut(s) 519
PspN4I GGNNCC 1 cut(s) 68
PspPI GGNCC 1 cut(s) 465
PvuII CAGCTG 1 cut(s) 401
RsaI GTAC 1 cut(s) 170
RsaNI GTAC 1 cut(s) 169
RseI CAYNNNNRTG 2 cut(s) 107, 131
SacI GAGCTC 1 cut(s) 101
SaqAI TTAA 5 cut(s) 255, 342, 477, 525, 535
SatI GCNGC 2 cut(s) 36, 399
Sau96I GGNCC 1 cut(s) 465
ScaI AGTACT 1 cut(s) 170
SchI GAGTC 1 cut(s) 450
ScrFI CCNGG 2 cut(s) 19, 521
SduI GDGCHC 1 cut(s) 101
SetI ASST 8 cut(s) 10, 54, 101, 150, 210, 246, 403, 525
SfaNI GCATC 2 cut(s) 103, 184
SfcI CTRYAG 2 cut(s) 9, 402
SmiMI CAYNNNNRTG 2 cut(s) 107, 131
SmlI CTYRAG 1 cut(s) 143
SmoI CTYRAG 1 cut(s) 143
Sse9I AATT 2 cut(s) 57, 373
SspMI CTAG 1 cut(s) 357
SstI GAGCTC 1 cut(s) 101
StyD4I CCNGG 2 cut(s) 17, 519
StyI CCWWGG 2 cut(s) 231, 384
TasI AATT 2 cut(s) 57, 373
TatI WGTACW 1 cut(s) 168
Tru1I TTAA 5 cut(s) 255, 342, 477, 525, 535
Tru9I TTAA 5 cut(s) 255, 342, 477, 525, 535
TseI GCWGC 2 cut(s) 35, 398
TspDTI ATGAA 1 cut(s) 354
TspGWI ACGGA 1 cut(s) 76
VspI ATTAAT 1 cut(s) 477
XapI RAATTY 1 cut(s) 57
XmnI GAANNNNTTC 1 cut(s) 264
XspI CTAG 1 cut(s) 357
ZrmI AGTACT 1 cut(s) 170
Zsp2I ATGCAT 1 cut(s) 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.