Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0015822

Physical Location & Seq

Genomic Coordinates Reverse (-)

36900530 .. 36901906

Gene Structure 1377 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1278400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1377 bp

ATGAAAAAAGGAAACATATTGATGCACAAGTACGAGCTGGGACGGTTGCTTGGAAAGGGGACATTTGCCAAGGTTTACCATGCCCGAAACCTGAGCTCCAGCCAGAGCGTTGCGATTAAGATAATCGACAAGGAGAAGGTGCAGCATGTCGGATTGATTGATCAGATTAAGAGGGAAATCTCCGTGATGCGCCTTGTTAGGCACCCGAATGTGGTTCAGCTCTTTGAGGTCATGGCCAGCAAGACCAAGATTTACTTTGCCATGGAGTACGTGAAAGGTGGGGAACTTTTCGACAAGGTTGCCAAAGGGAAGCTCAAGGAAGACGTGGCGCGGAAATACTTTCAGCAACTGATTGGGGCTGTTGATTACTGCCACAGTCGAGGGGTCTATCACCGGGACATCAAGCCAGAGAATCTCTTGGTGGATGAGAATGGAAACCTCAAAGTTTCGGATTTTGGATTGAGCGCATTGTGGGAGTCTAGGGGACAAGATGGCCTCCTCCACACCACGTGTGGAACGCCAGCGTATGTGGCACCGGAGGTAATAAACAAGAAAGGTTATGATGGCGCAAAGGCAGATACATGGTCTTGTGGGGTTGTCCTCTTTGTTCTACTGGCCGGTTTCCTGCCATTCCATGACACCAATCTCATGGAAATGTATAGGAAAACCAGCAGAGGAGACTTCAAGAGCCCTCAGTGGTTTCCCCCAGAAGTAAGTAAGCTTCTTTCGAGGATTCTTGATCCAAATCCAAGCTCAAGAATAAGTGTTGACAAGATCATGGAGAATAGTTGGTTCAAGAAGGGGTTTAAACAAATCGAAGCGCCTTTAGCCCTTCCGTGTGATCCCAGCACCTCAATCCGCGACGTCCATGAGGCATTTGCATCAACAAGTCATTCAGCTGGAGAAGGTAATGCTAGCGGAGCAGGAACAAGCTCCATGAGGCCAACATGTTTCAATGCGTTCGACATCATATCTCTCTCACCAGGTTTTGACCTATCCGGTTTGTTTGAGAAGGATATGAATCACAGACCACAGGCGCGATTCACGAGCACAAAACCAGCCGCGACAATTGTTTCAAAATTCGAAGCCATAGCACAGACGGAGAGGTTCAAATGCATGAAAAAAGATGGAACCCTTAAACTGCAGGGTAGCAAGGAAGGCAGGAAAGGGCAGCTAGGCATCGATGCTGAGATTTTCGAGGTCACGCCTTCATTTCATGTCGTGGAGATGAAGAAAACGGCTGGGGACACATTGGAATACATGGAGTTCTGTGACCATGACTTGAAACCCTCTCTTAAGGATATAGTATGGACTTGGCAAGGAACAGAGCAGCAACAGCCACCACCGCCAGTAGTAGCGCCTGAACTCGTTTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018107 GO:0018193 GO:0018209 GO:0018210 GO:0019538 GO:0023052 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0097305 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04068 ko04113 ko04138 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 ko05418 map04068 map04113 map04138 map04140 map04150 map04151 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410 map05418

Pfam Domains

Protein Families

NAF Pkinase

Protein Analysis

459

Amino Acids

51.13

Weight (kDa)

8.82

Isoelectric Point (pI)

30.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	11 - 265	1.1e-75	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	12 - 261	1.7e-40	Protein tyrosine and serine/threonine kinase
Kinase-like	PF14531	103 - 253	1.7e-08	Kinase-like
NAF	PF03822	315 - 374	2.5e-21	NAF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015822)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G45820
fragaria_vesca	FvH4_3g07630
malus_domestica	MD05G1298900.v1.1 MD10G1278400.v1.1
prunus_persica	Prupe.4G062900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0011061
rosa_laevigata	RLG00000031806
rosa_multiflora	Rmu_sc0003242.1_g000007
rosa_roxburghii	Rroxscaffold_1G00065350
rosa_rugosa	Rorug04G0454900
rosa_samantha	Rh5AG087000 Rh5BG081900 Rh5CG094700
rosa_wichuraiana	Rw0G000150 Rw0G017800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	867
AccB1I	GGYRCC	2 cut(s)	201, 532
AccII	CGCG	4 cut(s)	331, 861, 1039, 1064
AciI	CCGC	5 cut(s)	331, 859, 918, 1062, 1346
AclWI	GGATC	2 cut(s)	734, 836
AcoI	YGGCCR	2 cut(s)	234, 615
AcsI	RAATTY	1 cut(s)	1079
AcvI	CACGTG	1 cut(s)	510
AcyI	GRCGYC	1 cut(s)	864
AdeI	CACNNNGTG	1 cut(s)	510
AfaI	GTAC	2 cut(s)	32, 269
AfiI	CCNNNNNNNGG	1 cut(s)	211
AflII	CTTAAG	1 cut(s)	1295
AflIII	ACRYGT	2 cut(s)	509, 947
AgsI	TTSAA	6 cut(s)	685, 796, 955, 1077, 1111, 1285
AjiI	CACGTC	1 cut(s)	325
AjnI	CCWGG	1 cut(s)	982
AluBI	AGCT	9 cut(s)	37, 96, 220, 313, 721, 753, 899, 933, 1174
AluI	AGCT	9 cut(s)	37, 96, 220, 313, 721, 753, 899, 933, 1174
Alw21I	GWGCWC	2 cut(s)	98, 1052
Alw26I	GTCTC	1 cut(s)	672
AlwI	GGATC	2 cut(s)	734, 836
AlwNI	CAGNNNCTG	1 cut(s)	349
AoxI	GGCC	4 cut(s)	234, 493, 615, 941
ApeKI	GCWGC	3 cut(s)	142, 1171, 1330
ApoI	RAATTY	1 cut(s)	1079
AspLEI	GCGC	7 cut(s)	192, 331, 467, 569, 823, 1039, 1360
AsuC2I	CCSGG	1 cut(s)	395
AsuHPI	GGTGA	2 cut(s)	383, 972
AsuII	TTCGAA	1 cut(s)	1083
AsuNHI	GCTAGC	1 cut(s)	914
BalI	TGGCCA	1 cut(s)	236
BanI	GGYRCC	2 cut(s)	201, 532
BanII	GRGCYC	2 cut(s)	98, 692
BarI	GAAGNNNNNNTAC	2 cut(s)	705, 737
BauI	CACGAG	1 cut(s)	1045
BbrPI	CACGTG	1 cut(s)	510
BbsI	GAAGAC	1 cut(s)	327
Bbv12I	GWGCWC	2 cut(s)	98, 1052
BbvI	GCAGC	3 cut(s)	154, 1183, 1342
BccI	CCATC	3 cut(s)	485, 557, 1121
BceAI	ACGGC	1 cut(s)	1254
BcgI	CGANNNNNNTGC	2 cut(s)	281, 315
BciT130I	CCWGG	1 cut(s)	984
BclI	TGATCA	1 cut(s)	160
BcnI	CCSGG	1 cut(s)	395
BcoDI	GTCTC	1 cut(s)	672
BfaI	CTAG	3 cut(s)	480, 915, 1175
BfmI	CTRYAG	1 cut(s)	1142
BfoI	RGCGCY	2 cut(s)	824, 1361
BfrI	CTTAAG	1 cut(s)	1295
BisI	GCNGC	4 cut(s)	143, 1062, 1172, 1331
BlsI	GCNGC	4 cut(s)	144, 1063, 1173, 1332
Bme1390I	CCNGG	2 cut(s)	395, 984
BmgBI	CACGTC	1 cut(s)	325
BmiI	GGNNCC	3 cut(s)	203, 534, 1132
BmrFI	CCNGG	2 cut(s)	395, 984
BmsI	GCATC	5 cut(s)	12, 177, 890, 1174, 1188
BmtI	GCTAGC	1 cut(s)	918
BpiI	GAAGAC	1 cut(s)	327
BpmI	CTGGAG	2 cut(s)	82, 921
Bpu10I	CCTNAGC	1 cut(s)	92
Bpu14I	TTCGAA	1 cut(s)	1083
BpuEI	CTTGAG	2 cut(s)	299, 739
BpuMI	CCSGG	1 cut(s)	395
Bsa29I	ATCGAT	1 cut(s)	1182
BsaAI	YACGTR	2 cut(s)	271, 510
BsaBI	GATNNNNATC	2 cut(s)	744, 1020
BsaHI	GRCGYC	1 cut(s)	864
BsaJI	CCNNGG	2 cut(s)	69, 261
BsaWI	WCCGGW	2 cut(s)	535, 998
BsaXI	ACNNNNNCTCC	2 cut(s)	80, 110
Bsc4I	CCNNNNNNNGG	1 cut(s)	211
Bse118I	RCCGGY	1 cut(s)	617
Bse1I	ACTGG	2 cut(s)	618, 1349
Bse8I	GATNNNNATC	2 cut(s)	744, 1020
BseBI	CCWGG	1 cut(s)	984
BseCI	ATCGAT	1 cut(s)	1182
BseDI	CCNNGG	2 cut(s)	69, 261
BseGI	GGATG	1 cut(s)	430
BseJI	GATNNNNATC	2 cut(s)	744, 1020
BseLI	CCNNNNNNNGG	1 cut(s)	211
BseMII	CTCAG	3 cut(s)	83, 707, 1179
BseNI	ACTGG	2 cut(s)	618, 1349
BseRI	GAGGAG	2 cut(s)	488, 690
BseXI	GCAGC	3 cut(s)	154, 1183, 1342
BseYI	CCCAGC	3 cut(s)	37, 845, 1241
BsgI	GTGCAG	1 cut(s)	161
Bsh1236I	CGCG	4 cut(s)	331, 861, 1039, 1064
BshFI	GGCC	4 cut(s)	236, 495, 617, 943
BshNI	GGYRCC	2 cut(s)	201, 532
BshVI	ATCGAT	1 cut(s)	1182
BsiHKAI	GWGCWC	2 cut(s)	98, 1052
BsiSI	CCGG	4 cut(s)	394, 536, 618, 999
BslFI	GGGAC	5 cut(s)	54, 73, 410, 498, 1259
BslI	CCNNNNNNNGG	1 cut(s)	211
BsmAI	GTCTC	1 cut(s)	672
BsmFI	GGGAC	5 cut(s)	54, 73, 410, 498, 1259
BsnI	GGCC	4 cut(s)	236, 495, 617, 943
Bsp119I	TTCGAA	1 cut(s)	1083
Bsp1286I	GDGCHC	3 cut(s)	98, 692, 1052
Bsp143I	GATC	4 cut(s)	160, 739, 774, 841
Bsp19I	CCATGG	1 cut(s)	261
BspACI	CCGC	5 cut(s)	331, 859, 918, 1062, 1346
BspANI	GGCC	4 cut(s)	236, 495, 617, 943
BspCNI	CTCAG	3 cut(s)	84, 706, 1180
BspDI	ATCGAT	1 cut(s)	1182
BspFNI	CGCG	4 cut(s)	331, 861, 1039, 1064
BspLI	GGNNCC	3 cut(s)	203, 534, 1132
BspMAI	CTGCAG	1 cut(s)	1146
BspOI	GCTAGC	1 cut(s)	918
BspPI	GGATC	2 cut(s)	734, 836
BspT104I	TTCGAA	1 cut(s)	1083
BspT107I	GGYRCC	2 cut(s)	201, 532
BspTI	CTTAAG	1 cut(s)	1295
BsrFI	RCCGGY	1 cut(s)	617
BsrI	ACTGG	2 cut(s)	618, 1349
BssAI	RCCGGY	1 cut(s)	617
BssECI	CCNNGG	2 cut(s)	69, 261
BssMI	GATC	4 cut(s)	160, 739, 774, 841
BssNI	GRCGYC	1 cut(s)	864
BssSI	CACGAG	1 cut(s)	1045
BssT1I	CCWWGG	2 cut(s)	69, 261
Bst2BI	CACGAG	1 cut(s)	1045
Bst2UI	CCWGG	1 cut(s)	984
Bst4CI	ACNGT	2 cut(s)	45, 377
BstACI	GRCGYC	1 cut(s)	864
BstAFI	CTTAAG	1 cut(s)	1295
BstBAI	YACGTR	2 cut(s)	271, 510
BstBI	TTCGAA	1 cut(s)	1083
BstC8I	GCNNGC	3 cut(s)	238, 522, 916
BstDEI	CTNAG	4 cut(s)	92, 693, 1188, 1374
BstDSI	CCRYGG	1 cut(s)	261
BstF5I	GGATG	1 cut(s)	430
BstFNI	CGCG	4 cut(s)	331, 861, 1039, 1064
BstH2I	RGCGCY	2 cut(s)	824, 1361
BstHHI	GCGC	7 cut(s)	192, 331, 467, 569, 823, 1039, 1360
BstKTI	GATC	4 cut(s)	163, 742, 777, 844
BstMAI	GTCTC	1 cut(s)	672
BstMBI	GATC	4 cut(s)	160, 739, 774, 841
BstMWI	GCNNNNNNNGC	6 cut(s)	530, 827, 920, 1158, 1336, 1345
BstNI	CCWGG	1 cut(s)	984
BstNSI	RCATGY	2 cut(s)	149, 951
BstSCI	CCNGG	2 cut(s)	393, 982
BstSFI	CTRYAG	1 cut(s)	1142
BstUI	CGCG	4 cut(s)	331, 861, 1039, 1064
BstV1I	GCAGC	3 cut(s)	154, 1183, 1342
BstV2I	GAAGAC	1 cut(s)	327
BstXI	CCANNNNNNTGG	1 cut(s)	649
Bsu15I	ATCGAT	1 cut(s)	1182
BsuRI	GGCC	4 cut(s)	236, 495, 617, 943
BsuTUI	ATCGAT	1 cut(s)	1182
BtgI	CCRYGG	1 cut(s)	261
BtrI	CACGTC	1 cut(s)	325
BtsCI	GGATG	1 cut(s)	430
BtsIMutI	CAGTG	1 cut(s)	701
Cac8I	GCNNGC	3 cut(s)	238, 522, 916
CaiI	CAGNNNCTG	1 cut(s)	349
CfoI	GCGC	7 cut(s)	192, 331, 467, 569, 823, 1039, 1360
Cfr10I	RCCGGY	1 cut(s)	617
ClaI	ATCGAT	1 cut(s)	1182
CsiI	ACCWGGT	1 cut(s)	982
Csp6I	GTAC	2 cut(s)	31, 268
CviQI	GTAC	2 cut(s)	31, 268
DdeI	CTNAG	4 cut(s)	92, 693, 1188, 1374
DpnI	GATC	4 cut(s)	162, 741, 776, 843
DpnII	GATC	4 cut(s)	160, 739, 774, 841
DraI	TTTAAA	1 cut(s)	808
DraIII	CACNNNGTG	1 cut(s)	510
EaeI	YGGCCR	2 cut(s)	234, 615
Ecl136II	GAGCTC	1 cut(s)	96
Eco130I	CCWWGG	2 cut(s)	69, 261
Eco24I	GRGCYC	2 cut(s)	98, 692
Eco53kI	GAGCTC	1 cut(s)	96
Eco72I	CACGTG	1 cut(s)	510
EcoICRI	GAGCTC	1 cut(s)	96
EcoRII	CCWGG	1 cut(s)	982
EcoT14I	CCWWGG	2 cut(s)	69, 261
EcoT22I	ATGCAT	1 cut(s)	1118
EcoT38I	GRGCYC	2 cut(s)	98, 692
ErhI	CCWWGG	2 cut(s)	69, 261
FalI	AAGNNNNNCTT	2 cut(s)	239, 271
FaqI	GGGAC	5 cut(s)	54, 73, 410, 498, 1259
FbaI	TGATCA	1 cut(s)	160
Fnu4HI	GCNGC	4 cut(s)	143, 1062, 1172, 1331
FokI	GGATG	1 cut(s)	437
FriOI	GRGCYC	2 cut(s)	98, 692
Fsp4HI	GCNGC	4 cut(s)	143, 1062, 1172, 1331
FspBI	CTAG	3 cut(s)	480, 915, 1175
GlaI	GCGC	7 cut(s)	191, 330, 466, 568, 822, 1038, 1359
GluI	GCNGC	4 cut(s)	143, 1062, 1172, 1331
GsaI	CCCAGC	3 cut(s)	41, 849, 1245
GsuI	CTGGAG	2 cut(s)	82, 921
HaeII	RGCGCY	2 cut(s)	824, 1361
HaeIII	GGCC	4 cut(s)	236, 495, 617, 943
HapII	CCGG	4 cut(s)	394, 536, 618, 999
HhaI	GCGC	7 cut(s)	192, 331, 467, 569, 823, 1039, 1360
Hin1I	GRCGYC	1 cut(s)	864
Hin6I	GCGC	7 cut(s)	190, 329, 465, 567, 821, 1037, 1358
HinP1I	GCGC	7 cut(s)	190, 329, 465, 567, 821, 1037, 1358
HincII	GTYRAC	1 cut(s)	769
HindII	GTYRAC	1 cut(s)	769
HindIII	AAGCTT	1 cut(s)	719
HinfI	GANTC	5 cut(s)	412, 476, 733, 1021, 1041
HpaII	CCGG	4 cut(s)	394, 536, 618, 999
HphI	GGTGA	2 cut(s)	383, 972
Hpy166II	GTNNAC	2 cut(s)	76, 769
Hpy188I	TCNGA	3 cut(s)	152, 165, 451
Hpy188III	TCNNGA	5 cut(s)	685, 737, 756, 796, 1045
Hpy8I	GTNNAC	2 cut(s)	76, 769
Hpy99I	CGWCG	1 cut(s)	866
HpyAV	CCTTC	7 cut(s)	130, 793, 842, 899, 1006, 1151, 1218
HpyCH4III	ACNGT	2 cut(s)	45, 377
HpyCH4IV	ACGT	4 cut(s)	270, 324, 509, 864
HpyCH4V	TGCA	5 cut(s)	25, 142, 881, 1116, 1144
HpyF10VI	GCNNNNNNNGC	6 cut(s)	530, 827, 920, 1158, 1336, 1345
HpyF3I	CTNAG	4 cut(s)	92, 693, 1188, 1374
HpySE526I	ACGT	4 cut(s)	270, 324, 509, 864
Hsp92I	GRCGYC	1 cut(s)	864
HspAI	GCGC	7 cut(s)	190, 329, 465, 567, 821, 1037, 1358
Ksp22I	TGATCA	1 cut(s)	160
Kzo9I	GATC	4 cut(s)	160, 739, 774, 841
LmnI	GCTCC	3 cut(s)	101, 920, 938
Lsp1109I	GCAGC	3 cut(s)	154, 1183, 1342
LweI	GCATC	5 cut(s)	12, 177, 890, 1174, 1188
MabI	ACCWGGT	1 cut(s)	982
MaeI	CTAG	3 cut(s)	480, 915, 1175
MaeII	ACGT	4 cut(s)	270, 324, 509, 864
MaeIII	GTNAC	2 cut(s)	1201, 1271
MalI	GATC	4 cut(s)	162, 741, 776, 843
MboI	GATC	4 cut(s)	160, 739, 774, 841
MboII	GAAGA	2 cut(s)	332, 1243
MfeI	CAATTG	1 cut(s)	1068
MhlI	GDGCHC	3 cut(s)	98, 692, 1052
MlsI	TGGCCA	1 cut(s)	236
MluCI	AATT	2 cut(s)	1068, 1079
MluNI	TGGCCA	1 cut(s)	236
MlyI	GAGTC	1 cut(s)	485
MmeI	TCCRAC	1 cut(s)	130
Mox20I	TGGCCA	1 cut(s)	236
Mph1103I	ATGCAT	1 cut(s)	1118
MscI	TGGCCA	1 cut(s)	236
MseI	TTAA	5 cut(s)	117, 168, 807, 1137, 1296
MslI	CAYNNNNRTG	3 cut(s)	20, 207, 653
Msp20I	TGGCCA	1 cut(s)	236
MspA1I	CMGCKG	1 cut(s)	899
MspCI	CTTAAG	1 cut(s)	1295
MspI	CCGG	4 cut(s)	394, 536, 618, 999
MspR9I	CCNGG	2 cut(s)	395, 984
MssI	GTTTAAAC	1 cut(s)	808
MunI	CAATTG	1 cut(s)	1068
MvaI	CCWGG	1 cut(s)	984
MvnI	CGCG	4 cut(s)	331, 861, 1039, 1064
MwoI	GCNNNNNNNGC	6 cut(s)	530, 827, 920, 1158, 1336, 1345
NciI	CCSGG	1 cut(s)	395
NcoI	CCATGG	1 cut(s)	261
NdeII	GATC	4 cut(s)	160, 739, 774, 841
NheI	GCTAGC	1 cut(s)	914
NlaIV	GGNNCC	3 cut(s)	203, 534, 1132
NmuCI	GTSAC	2 cut(s)	1201, 1271
NsiI	ATGCAT	1 cut(s)	1118
NspI	RCATGY	2 cut(s)	149, 951
NspV	TTCGAA	1 cut(s)	1083
PciI	ACATGT	1 cut(s)	947
PfeI	GAWTC	4 cut(s)	412, 733, 1021, 1041
PkrI	GCNGC	4 cut(s)	144, 1063, 1173, 1332
PleI	GAGTC	1 cut(s)	484
PmaCI	CACGTG	1 cut(s)	510
PmeI	GTTTAAAC	1 cut(s)	808
PmlI	CACGTG	1 cut(s)	510
PpsI	GAGTC	1 cut(s)	484
Ppu21I	YACGTR	2 cut(s)	271, 510
PscI	ACATGT	1 cut(s)	947
Psp124BI	GAGCTC	1 cut(s)	98
Psp6I	CCWGG	1 cut(s)	982
PspCI	CACGTG	1 cut(s)	510
PspFI	CCCAGC	3 cut(s)	37, 845, 1241
PspGI	CCWGG	1 cut(s)	982
PspN4I	GGNNCC	3 cut(s)	203, 534, 1132
PstI	CTGCAG	1 cut(s)	1146
PstNI	CAGNNNCTG	1 cut(s)	349
PvuII	CAGCTG	1 cut(s)	899
RsaI	GTAC	2 cut(s)	32, 269
RsaNI	GTAC	2 cut(s)	31, 268
RseI	CAYNNNNRTG	3 cut(s)	20, 207, 653
SacI	GAGCTC	1 cut(s)	98
SaqAI	TTAA	5 cut(s)	117, 168, 807, 1137, 1296
SatI	GCNGC	4 cut(s)	143, 1062, 1172, 1331
Sau3AI	GATC	4 cut(s)	160, 739, 774, 841
SchI	GAGTC	1 cut(s)	485
ScrFI	CCNGG	2 cut(s)	395, 984
SduI	GDGCHC	3 cut(s)	98, 692, 1052
SexAI	ACCWGGT	1 cut(s)	982
SfaNI	GCATC	5 cut(s)	12, 177, 890, 1174, 1188
SfcI	CTRYAG	1 cut(s)	1142
SfuI	TTCGAA	1 cut(s)	1083
SmiMI	CAYNNNNRTG	3 cut(s)	20, 207, 653
SmlI	CTYRAG	3 cut(s)	314, 754, 1295
SmoI	CTYRAG	3 cut(s)	314, 754, 1295
Sse9I	AATT	2 cut(s)	1068, 1079
SsiI	CCGC	5 cut(s)	331, 859, 918, 1062, 1346
SspMI	CTAG	3 cut(s)	480, 915, 1175
SstI	GAGCTC	1 cut(s)	98
StyD4I	CCNGG	2 cut(s)	393, 982
StyI	CCWWGG	2 cut(s)	69, 261
TaaI	ACNGT	2 cut(s)	45, 377
TaiI	ACGT	4 cut(s)	273, 327, 512, 867
TaqI	TCGA	9 cut(s)	126, 291, 379, 728, 816, 963, 1083, 1182, 1197
TasI	AATT	2 cut(s)	1068, 1079
TauI	GCSGC	1 cut(s)	1064
TfiI	GAWTC	4 cut(s)	412, 733, 1021, 1041
Tru1I	TTAA	5 cut(s)	117, 168, 807, 1137, 1296
Tru9I	TTAA	5 cut(s)	117, 168, 807, 1137, 1296
TscAI	CASTG	1 cut(s)	701
TseFI	GTSAC	2 cut(s)	1201, 1271
TseI	GCWGC	3 cut(s)	142, 1171, 1330
Tsp45I	GTSAC	2 cut(s)	1201, 1271
TspDTI	ATGAA	6 cut(s)	17, 1034, 1133, 1200, 1205, 1244
TspGWI	ACGGA	3 cut(s)	172, 825, 1115
TspRI	CASTG	1 cut(s)	701
Vha464I	CTTAAG	1 cut(s)	1295
XapI	RAATTY	1 cut(s)	1079
XceI	RCATGY	2 cut(s)	149, 951
XcmI	CCANNNNNNNNNTGG	1 cut(s)	509
XspI	CTAG	3 cut(s)	480, 915, 1175
ZraI	GACGTC	1 cut(s)	865
Zsp2I	ATGCAT	1 cut(s)	1118

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.