Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0015822

Physical Location & Seq

Genomic Coordinates Forward (+)

7495637 .. 7497040

Gene Structure 1404 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG094700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1404 bp

ATGTCCAATATGGAGAACAAGAAAGGAAACATATTGATGCAGAAGTACGAGCTGGGGCGCCTTCTCGGGAAAGGTACATTTGCAAAGGTTTACCATGCTCGAAACATGAAGACCGGCCAAAGTGTTGCCATTAAGATCATAGACAAGGAGAAGGTGCAACAAGTGGGATTGATTGATCAAATCAAGCGTGAAATCTCTGTGATGCGCCTTGTTAGGCACCCCAATGTTGTTCAGCTCTATGAAGTGATGGCCAGCAAGACCAAAATCTACTTTGCCATGGAGTATGTGAGAGGTGGTGAGCTCTTCAACAAGGTTGCCAGGGGAAAGCTCAAGGAAGACATGGCTAGAAAATACTTTCAACAGTTGATTGGCGCAGTTGATTACTGCCACAGCCGCGGAGTTTATCACCGTGACATCAAGCCAGAGAATCTCCTGGTTGATGAGCATGGTAACCTCAAGGTTTCAGATTTTGGGCTGAGTGCATTGTGGGAGTCGAGAGGTCAAGATGGTCTGCTGCACACAACTTGTGGAACTCCTGCTTATGTAGCACCAGAAGTGATCAACAAGAAAGGTTATGATGGTGCCAAGGCTGATATATGGTCATGTGGGGTTGTCCTTTACGTTCTTTTGGCAGGTTTTCTTCCATTCCATGACACAAATCTCATGGAAATGTACAGGAAGATCAGCAGAGGAGACTTCAAGAGTCCGCAATGGTTCCCTCCAGAGGTTCGTAAGCTTCTTGCACGGATTCTTGATCCAAATGCCACCACGAGAATAAGTGTGGACAAGATCATGGAGAACAGTTGGTTCAAGAAGGGGTTTAAGCACATTGATGGCGCCCCATTACCGATTCCAGGTGATCCAAACACATCTATCACTGATGTGCATTCTGCTTTTGGATCACCAGACAGTTCTGAAAGCAGTTCTAATAGGAAACCAGCAATTACTACTGCAGCAGCAAGCCCCATGAAGCCAACTAACTTCAATGCCTTTGACATCATATCTCTCTCACCCGGATTTGATCTATCCGGTTTGTTTGAGGGTGATATAAAGCAAAGATCATCACAGTCACGATTCACCACTACAAAACCAGCCTCTACTATTGTTTCAAAATTTGAAGAGATAGCGCAGATGGAGAGATTCAGATGCATGAAGCAGGACGGAACTGTCAAGTTGCAGGGCAGCAGGGAAGGAAGAAAAGGGCAGCTTGGTATTGATGCTGAGATTTTCGAGGTCACGCCTTCGTTTTTTGTTGTGGAGGTGAAGAAAACAGCTGGGGACACATTGGAATACATGCAATTCTATGACCAAGATTTAAAGCCCTCTCTTAAAGACATAGTATGGACTTGGCAAGGAAATGATCCACAGCAACAACATCAGCCAGCAACCCAAGTTATTTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018107 GO:0018193 GO:0018209 GO:0018210 GO:0019538 GO:0023052 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0097305 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04068 ko04113 ko04138 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 ko05418 map04068 map04113 map04138 map04140 map04150 map04151 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410 map05418

Pfam Domains

Protein Families

NAF Pkinase

Protein Analysis

467

Amino Acids

52.55

Weight (kDa)

9.15

Isoelectric Point (pI)

33.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	16 - 270	1.4e-76	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	17 - 266	1.9e-41	Protein tyrosine and serine/threonine kinase
Kinase-like	PF14531	106 - 258	2.2e-09	Kinase-like
NAF	PF03822	325 - 385	6.9e-21	NAF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015822)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G45820
fragaria_vesca	FvH4_3g07630
malus_domestica	MD05G1298900.v1.1 MD10G1278400.v1.1
prunus_persica	Prupe.4G062900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0011061
rosa_laevigata	RLG00000031806
rosa_multiflora	Rmu_sc0003242.1_g000007
rosa_roxburghii	Rroxscaffold_1G00065350
rosa_rugosa	Rorug04G0454900
rosa_samantha	Rh5AG087000 Rh5BG081900 Rh5CG094700
rosa_wichuraiana	Rw0G000150 Rw0G017800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1166
Acc36I	ACCTGC	1 cut(s)	623
AccB1I	GGYRCC	4 cut(s)	57, 216, 581, 836
AccII	CGCG	1 cut(s)	396
AciI	CCGC	3 cut(s)	394, 396, 707
AclWI	GGATC	4 cut(s)	749, 854, 907, 1355
AcoI	YGGCCR	2 cut(s)	115, 249
AcsI	RAATTY	1 cut(s)	1112
AcyI	GRCGYC	2 cut(s)	58, 837
AfaI	GTAC	3 cut(s)	47, 76, 674
AfiI	CCNNNNNNNGG	2 cut(s)	724, 854
AgsI	TTSAA	7 cut(s)	307, 359, 700, 811, 985, 1110, 1118
AjnI	CCWGG	3 cut(s)	317, 432, 853
AleI	CACNNNNGTG	1 cut(s)	881
AluBI	AGCT	7 cut(s)	52, 235, 301, 328, 736, 1207, 1274
AluI	AGCT	7 cut(s)	52, 235, 301, 328, 736, 1207, 1274
Alw21I	GWGCWC	1 cut(s)	303
Alw26I	GTCTC	1 cut(s)	687
AlwI	GGATC	4 cut(s)	749, 854, 907, 1355
Ama87I	CYCGRG	1 cut(s)	65
AoxI	GGCC	2 cut(s)	115, 249
ApeKI	GCWGC	5 cut(s)	514, 953, 956, 1182, 1204
ApoI	RAATTY	1 cut(s)	1112
AspLEI	GCGC	5 cut(s)	60, 207, 374, 839, 1129
AsuC2I	CCSGG	1 cut(s)	1014
AsuHPI	GGTGA	8 cut(s)	308, 398, 869, 894, 1002, 1055, 1069, 1273
AvaI	CYCGRG	1 cut(s)	65
BalI	TGGCCA	1 cut(s)	251
BanI	GGYRCC	4 cut(s)	57, 216, 581, 836
BanII	GRGCYC	1 cut(s)	303
BauI	CACGAG	1 cut(s)	769
BbsI	GAAGAC	2 cut(s)	116, 342
Bbv12I	GWGCWC	1 cut(s)	303
BbvI	GCAGC	5 cut(s)	501, 965, 968, 1194, 1216
BccI	CCATC	5 cut(s)	241, 500, 572, 827, 1126
BciT130I	CCWGG	3 cut(s)	319, 434, 855
BclI	TGATCA	2 cut(s)	175, 558
BcnI	CCSGG	1 cut(s)	1014
BcoDI	GTCTC	1 cut(s)	687
BfaI	CTAG	1 cut(s)	345
BfmI	CTRYAG	1 cut(s)	951
BfoI	RGCGCY	2 cut(s)	61, 840
BfuAI	ACCTGC	1 cut(s)	623
BisI	GCNGC	6 cut(s)	394, 515, 954, 957, 1183, 1205
BlsI	GCNGC	6 cut(s)	395, 516, 955, 958, 1184, 1206
Bme1390I	CCNGG	4 cut(s)	319, 434, 855, 1014
BmeT110I	CYCGRG	1 cut(s)	65
BmiI	GGNNCC	5 cut(s)	59, 218, 583, 716, 838
BmrFI	CCNGG	4 cut(s)	319, 434, 855, 1014
BmsI	GCATC	4 cut(s)	27, 192, 1136, 1207
BpiI	GAAGAC	2 cut(s)	116, 342
BpmI	CTGGAG	1 cut(s)	705
BpuEI	CTTGAG	2 cut(s)	314, 440
BpuMI	CCSGG	1 cut(s)	1014
BsaHI	GRCGYC	2 cut(s)	58, 837
BsaJI	CCNNGG	4 cut(s)	276, 318, 394, 585
BsaWI	WCCGGW	1 cut(s)	1028
Bsc4I	CCNNNNNNNGG	2 cut(s)	724, 854
Bse118I	RCCGGY	1 cut(s)	113
Bse3DI	GCAATG	1 cut(s)	716
BseBI	CCWGG	3 cut(s)	319, 434, 855
BseDI	CCNNGG	4 cut(s)	276, 318, 394, 585
BseLI	CCNNNNNNNGG	2 cut(s)	724, 854
BseMI	GCAATG	1 cut(s)	716
BseMII	CTCAG	2 cut(s)	467, 1212
BseRI	GAGGAG	1 cut(s)	705
BseXI	GCAGC	5 cut(s)	501, 965, 968, 1194, 1216
BseYI	CCCAGC	2 cut(s)	52, 1274
BsgI	GTGCAG	1 cut(s)	500
Bsh1236I	CGCG	1 cut(s)	396
BshFI	GGCC	2 cut(s)	117, 251
BshNI	GGYRCC	4 cut(s)	57, 216, 581, 836
BsiHKAI	GWGCWC	1 cut(s)	303
BsiHKCI	CYCGRG	1 cut(s)	65
BsiSI	CCGG	3 cut(s)	114, 1014, 1029
BslFI	GGGAC	1 cut(s)	1292
BslI	CCNNNNNNNGG	2 cut(s)	724, 854
BsmAI	GTCTC	1 cut(s)	687
BsmFI	GGGAC	1 cut(s)	1292
BsmI	GAATGC	1 cut(s)	886
BsnI	GGCC	2 cut(s)	117, 251
BsoBI	CYCGRG	1 cut(s)	65
Bsp1286I	GDGCHC	1 cut(s)	303
Bsp1407I	TGTACA	1 cut(s)	672
Bsp19I	CCATGG	1 cut(s)	276
BspACI	CCGC	3 cut(s)	394, 396, 707
BspANI	GGCC	2 cut(s)	117, 251
BspCNI	CTCAG	2 cut(s)	468, 1213
BspFNI	CGCG	1 cut(s)	396
BspLI	GGNNCC	5 cut(s)	59, 218, 583, 716, 838
BspMAI	CTGCAG	1 cut(s)	955
BspMI	ACCTGC	1 cut(s)	623
BspPI	GGATC	4 cut(s)	749, 854, 907, 1355
BspQI	GCTCTTC	1 cut(s)	308
BspT107I	GGYRCC	4 cut(s)	57, 216, 581, 836
BsrDI	GCAATG	1 cut(s)	716
BsrFI	RCCGGY	1 cut(s)	113
BsrGI	TGTACA	1 cut(s)	672
BssAI	RCCGGY	1 cut(s)	113
BssECI	CCNNGG	4 cut(s)	276, 318, 394, 585
BssNI	GRCGYC	2 cut(s)	58, 837
BssSI	CACGAG	1 cut(s)	769
BssT1I	CCWWGG	2 cut(s)	276, 585
Bst2BI	CACGAG	1 cut(s)	769
Bst2UI	CCWGG	3 cut(s)	319, 434, 855
Bst4CI	ACNGT	6 cut(s)	363, 410, 803, 911, 1068, 1168
Bst6I	CTCTTC	2 cut(s)	308, 1113
BstACI	GRCGYC	2 cut(s)	58, 837
BstAUI	TGTACA	1 cut(s)	672
BstC8I	GCNNGC	3 cut(s)	253, 961, 1383
BstDEI	CTNAG	3 cut(s)	476, 1221, 1401
BstDSI	CCRYGG	2 cut(s)	276, 394
BstEII	GGTNACC	1 cut(s)	449
BstFNI	CGCG	1 cut(s)	396
BstH2I	RGCGCY	2 cut(s)	61, 840
BstHHI	GCGC	5 cut(s)	60, 207, 374, 839, 1129
BstMAI	GTCTC	1 cut(s)	687
BstMWI	GCNNNNNNNGC	2 cut(s)	393, 545
BstNI	CCWGG	3 cut(s)	319, 434, 855
BstNSI	RCATGY	1 cut(s)	1297
BstPI	GGTNACC	1 cut(s)	449
BstSCI	CCNGG	4 cut(s)	317, 432, 853, 1012
BstSFI	CTRYAG	1 cut(s)	951
BstUI	CGCG	1 cut(s)	396
BstV1I	GCAGC	5 cut(s)	501, 965, 968, 1194, 1216
BstV2I	GAAGAC	2 cut(s)	116, 342
BsuRI	GGCC	2 cut(s)	117, 251
BtgI	CCRYGG	2 cut(s)	276, 394
BtsIMutI	CAGTG	1 cut(s)	876
BveI	ACCTGC	1 cut(s)	623
Cac8I	GCNNGC	3 cut(s)	253, 961, 1383
CfoI	GCGC	5 cut(s)	60, 207, 374, 839, 1129
Cfr10I	RCCGGY	1 cut(s)	113
Cfr42I	CCGCGG	1 cut(s)	397
Csp6I	GTAC	3 cut(s)	46, 75, 673
CviQI	GTAC	3 cut(s)	46, 75, 673
DdeI	CTNAG	3 cut(s)	476, 1221, 1401
DinI	GGCGCC	2 cut(s)	59, 838
DraI	TTTAAA	1 cut(s)	1317
DrdI	GACNNNNNNGTC	1 cut(s)	1166
DseDI	GACNNNNNNGTC	1 cut(s)	1166
EaeI	YGGCCR	2 cut(s)	115, 249
Eam1104I	CTCTTC	2 cut(s)	308, 1113
EarI	CTCTTC	2 cut(s)	308, 1113
Ecl136II	GAGCTC	1 cut(s)	301
Eco130I	CCWWGG	2 cut(s)	276, 585
Eco24I	GRGCYC	1 cut(s)	303
Eco53kI	GAGCTC	1 cut(s)	301
Eco88I	CYCGRG	1 cut(s)	65
Eco91I	GGTNACC	1 cut(s)	449
EcoICRI	GAGCTC	1 cut(s)	301
EcoO65I	GGTNACC	1 cut(s)	449
EcoRII	CCWGG	3 cut(s)	317, 432, 853
EcoT14I	CCWWGG	2 cut(s)	276, 585
EcoT22I	ATGCAT	1 cut(s)	1151
EcoT38I	GRGCYC	1 cut(s)	303
EgeI	GGCGCC	2 cut(s)	59, 838
EheI	GGCGCC	2 cut(s)	59, 838
ErhI	CCWWGG	2 cut(s)	276, 585
FalI	AAGNNNNNCTT	2 cut(s)	1191, 1223
FaqI	GGGAC	1 cut(s)	1292
FbaI	TGATCA	2 cut(s)	175, 558
Fnu4HI	GCNGC	6 cut(s)	394, 515, 954, 957, 1183, 1205
FriOI	GRGCYC	1 cut(s)	303
Fsp4HI	GCNGC	6 cut(s)	394, 515, 954, 957, 1183, 1205
FspBI	CTAG	1 cut(s)	345
GlaI	GCGC	5 cut(s)	59, 206, 373, 838, 1128
GluI	GCNGC	6 cut(s)	394, 515, 954, 957, 1183, 1205
GsaI	CCCAGC	2 cut(s)	56, 1278
GsuI	CTGGAG	1 cut(s)	705
HaeII	RGCGCY	2 cut(s)	61, 840
HaeIII	GGCC	2 cut(s)	117, 251
HapII	CCGG	3 cut(s)	114, 1014, 1029
HhaI	GCGC	5 cut(s)	60, 207, 374, 839, 1129
Hin1I	GRCGYC	2 cut(s)	58, 837
Hin6I	GCGC	5 cut(s)	58, 205, 372, 837, 1127
HinP1I	GCGC	5 cut(s)	58, 205, 372, 837, 1127
HindIII	AAGCTT	1 cut(s)	734
HinfI	GANTC	7 cut(s)	427, 491, 703, 748, 850, 1074, 1140
HpaII	CCGG	3 cut(s)	114, 1014, 1029
HphI	GGTGA	8 cut(s)	308, 398, 869, 894, 1002, 1055, 1069, 1273
Hpy166II	GTNNAC	2 cut(s)	91, 784
Hpy188I	TCNGA	3 cut(s)	466, 916, 1145
Hpy188III	TCNNGA	8 cut(s)	67, 495, 503, 700, 722, 752, 811, 1071
Hpy8I	GTNNAC	2 cut(s)	91, 784
HpyAV	CCTTC	5 cut(s)	71, 145, 808, 1184, 1251
HpyCH4III	ACNGT	6 cut(s)	363, 410, 803, 911, 1068, 1168
HpyCH4IV	ACGT	1 cut(s)	621
HpyF10VI	GCNNNNNNNGC	2 cut(s)	393, 545
HpyF3I	CTNAG	3 cut(s)	476, 1221, 1401
HpySE526I	ACGT	1 cut(s)	621
Hsp92I	GRCGYC	2 cut(s)	58, 837
HspAI	GCGC	5 cut(s)	58, 205, 372, 837, 1127
KasI	GGCGCC	2 cut(s)	57, 836
Ksp22I	TGATCA	2 cut(s)	175, 558
KspI	CCGCGG	1 cut(s)	397
LguI	GCTCTTC	1 cut(s)	308
Lsp1109I	GCAGC	5 cut(s)	501, 965, 968, 1194, 1216
LweI	GCATC	4 cut(s)	27, 192, 1136, 1207
MaeI	CTAG	1 cut(s)	345
MaeII	ACGT	1 cut(s)	621
MaeIII	GTNAC	4 cut(s)	410, 449, 1068, 1234
MboII	GAAGA	8 cut(s)	121, 295, 347, 632, 691, 1130, 1206, 1276
MhlI	GDGCHC	1 cut(s)	303
MlsI	TGGCCA	1 cut(s)	251
MluCI	AATT	3 cut(s)	942, 1112, 1298
MluNI	TGGCCA	1 cut(s)	251
Mly113I	GGCGCC	2 cut(s)	58, 837
MlyI	GAGTC	2 cut(s)	500, 712
Mox20I	TGGCCA	1 cut(s)	251
Mph1103I	ATGCAT	1 cut(s)	1151
MscI	TGGCCA	1 cut(s)	251
MseI	TTAA	4 cut(s)	132, 822, 1316, 1329
MslI	CAYNNNNRTG	5 cut(s)	35, 222, 668, 831, 881
Msp20I	TGGCCA	1 cut(s)	251
MspA1I	CMGCKG	2 cut(s)	396, 1274
MspI	CCGG	3 cut(s)	114, 1014, 1029
MspR9I	CCNGG	4 cut(s)	319, 434, 855, 1014
Mva1269I	GAATGC	1 cut(s)	886
MvaI	CCWGG	3 cut(s)	319, 434, 855
MvnI	CGCG	1 cut(s)	396
MwoI	GCNNNNNNNGC	2 cut(s)	393, 545
NarI	GGCGCC	2 cut(s)	58, 837
NciI	CCSGG	1 cut(s)	1014
NcoI	CCATGG	1 cut(s)	276
NlaIV	GGNNCC	5 cut(s)	59, 218, 583, 716, 838
NmuCI	GTSAC	3 cut(s)	410, 1068, 1234
NsiI	ATGCAT	1 cut(s)	1151
NspI	RCATGY	1 cut(s)	1297
OliI	CACNNNNGTG	1 cut(s)	881
PciSI	GCTCTTC	1 cut(s)	308
PctI	GAATGC	1 cut(s)	886
PfeI	GAWTC	5 cut(s)	427, 748, 850, 1074, 1140
PkrI	GCNGC	6 cut(s)	395, 516, 955, 958, 1184, 1206
PleI	GAGTC	2 cut(s)	499, 711
PluTI	GGCGCC	2 cut(s)	61, 840
PpsI	GAGTC	2 cut(s)	499, 711
Psp124BI	GAGCTC	1 cut(s)	303
Psp6I	CCWGG	3 cut(s)	317, 432, 853
PspEI	GGTNACC	1 cut(s)	449
PspFI	CCCAGC	2 cut(s)	52, 1274
PspGI	CCWGG	3 cut(s)	317, 432, 853
PspN4I	GGNNCC	5 cut(s)	59, 218, 583, 716, 838
PstI	CTGCAG	1 cut(s)	955
PvuII	CAGCTG	1 cut(s)	1274
RsaI	GTAC	3 cut(s)	47, 76, 674
RsaNI	GTAC	3 cut(s)	46, 75, 673
RseI	CAYNNNNRTG	5 cut(s)	35, 222, 668, 831, 881
SacI	GAGCTC	1 cut(s)	303
SacII	CCGCGG	1 cut(s)	397
SapI	GCTCTTC	1 cut(s)	308
SaqAI	TTAA	4 cut(s)	132, 822, 1316, 1329
SatI	GCNGC	6 cut(s)	394, 515, 954, 957, 1183, 1205
SchI	GAGTC	2 cut(s)	500, 712
ScrFI	CCNGG	4 cut(s)	319, 434, 855, 1014
SduI	GDGCHC	1 cut(s)	303
SfaNI	GCATC	4 cut(s)	27, 192, 1136, 1207
SfcI	CTRYAG	1 cut(s)	951
SfoI	GGCGCC	2 cut(s)	59, 838
Sfr303I	CCGCGG	1 cut(s)	397
SgrBI	CCGCGG	1 cut(s)	397
SmiMI	CAYNNNNRTG	5 cut(s)	35, 222, 668, 831, 881
SmlI	CTYRAG	2 cut(s)	329, 455
SmoI	CTYRAG	2 cut(s)	329, 455
Sse9I	AATT	3 cut(s)	942, 1112, 1298
SsiI	CCGC	3 cut(s)	394, 396, 707
SspDI	GGCGCC	2 cut(s)	57, 836
SspMI	CTAG	1 cut(s)	345
SstI	GAGCTC	1 cut(s)	303
StyD4I	CCNGG	4 cut(s)	317, 432, 853, 1012
StyI	CCWWGG	2 cut(s)	276, 585
TaaI	ACNGT	6 cut(s)	363, 410, 803, 911, 1068, 1168
TaiI	ACGT	1 cut(s)	624
TaqI	TCGA	3 cut(s)	100, 494, 1230
TasI	AATT	3 cut(s)	942, 1112, 1298
TatI	WGTACW	1 cut(s)	672
TauI	GCSGC	1 cut(s)	396
TfiI	GAWTC	5 cut(s)	427, 748, 850, 1074, 1140
Tru1I	TTAA	4 cut(s)	132, 822, 1316, 1329
Tru9I	TTAA	4 cut(s)	132, 822, 1316, 1329
TscAI	CASTG	1 cut(s)	883
TseFI	GTSAC	3 cut(s)	410, 1068, 1234
TseI	GCWGC	5 cut(s)	514, 953, 956, 1182, 1204
Tsp45I	GTSAC	3 cut(s)	410, 1068, 1234
TspDTI	ATGAA	4 cut(s)	122, 255, 983, 1166
TspGWI	ACGGA	2 cut(s)	760, 1176
TspRI	CASTG	1 cut(s)	883
XapI	RAATTY	1 cut(s)	1112
XceI	RCATGY	1 cut(s)	1297
XspI	CTAG	1 cut(s)	345
Zsp2I	ATGCAT	1 cut(s)	1151

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.