MD10G1305500.v1.1

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0016402
Physical Location & Seq
Reverse (-)
39166686 .. 39168824
2139 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1305500.v1.1.491

Sequence Viewer

Length: 2139 bp
ATGCAGCTACCCATACCCAAAGAGCTGATGAAGGTATGGGATGTATGGAATCTGAGGGGATGCATTCTCTTGAGCCTTGCTATTCAAGTTTTTCTAACGCTTTCCGCGCCGTTCAGGCAACAATGCAGAAGCATGTTGCTCCTAGGGTTGATCTGGTCGGCGTACTTGCTCTCCGACTGGGTTGCTGCTGTTTCCATTGGGGTAATCTCCAAAAGCCTGTCTGGGACTTGTGACTCTGGACATAATGAAGACCTTTTAGCATTCTGGGCTTCATTTCTTTTGGTGCATCTTGGTGGCCCCGATACGATTACTTCTTTTGCTCTGGAGGACAATGAGTTCTGGCTCAGGCACTTGCTTCAACTCATCCTACAAGTCCTGGCAGCTGCGTATAGTTTCTATTTGACACTTGTCGAAAACAAGCTTTGGATCCCGACGATCCTAGTGTTTGTTGCCGGTACTATCAAGTTTGGGGAGAGAACATGTTGTCTGTACCTTGCAAGCTTGGACCATTTTGGAGAGACTGTACTGCCAAATCCAGAACCAGGTCCTGACTATGAAGAGACCGTCGAGATATTTACTACAATGAGGTCAGTCAAGGTAGACATGCCGTCGCCAACAAGGCCATTTCACATTGGGAAAGGGATTAATCATAGAGAAAACCCCAATGTTGATGGAGTTTCTCAAGAATCTTTAGATGACATGGGGTTATTGCAGGAATCATACCACTTCTTCGAAAGCTTCAAAGGACTCATTGTTGGGTTCCTTCTGAGCTTCAATGATAGGGAGCGCAGTCGATATTTTTTTCTCGAAAGAACTCCCGTCAGTGCTTTTCAACTGATAGAATATGAGCTCAGCTTCATGTATGAGGTTCTCCATACCAAGGTATTTGTGGCGCGACACCAAATTGGCTACTTGCTCCGCTTAATTACCTTCTGTTCTACCATCGGTGCCTTGATGTTGTTCGCTTCAGCCGAAAAAGATGAGTTTAAGAAACATGAGTATGGTAAGTTTGACATTGTTCTTACTTATGCATTGCTTGTTGGAGCCATAGTCCTCGACACACTATCTGTGTTGTTGAAGCTCATTTTCTCTGACTGGACCATCATTGCCTTAAGAAACATCTGGAGCCAATGTTATATCCCTGCATGGATATTGAAAAGACGAAGGTGGTCTGGATCGGTGTCCCAGTATAATATGATAAGGAATTGCCTGGATGAGCGTCCAGAATGCGTGTTCGCTATTGGAGCTTGTGTGCGTGTTAGAGGAATCCTAGACAAAATAAAAGTTTCATTATTTTCTTCCTCTGAGGAATTCACGGATGAGCTCAAGGGCTTCGTGTTCAAGCAGCTGAGGATAAAATCTTTGGATGCAAACAACAGAAGAACCAACATGGAGGCATGCTTACAAAGAGGTGACTGGGCTCTCCAGCGGTCTTCAGGCTACAAGAAACTCAACTGGAGTGTAAAGGAGTATCAGTATGCAGAAAGTGTTCTTCTGTGGCACATTGCAACAGAACTCTGCTTTGCAAAATGCTCTGCAAAACCCTTGGAAGATGCTGACGGAAGACGAAAAATCTGCTTTGCAAAAACTGTGAAAGAACTCTGCTTTGCAAAATGCTTTGCAAAACCCTTGGAAGATGCTGACGGAAGACGAAAAATCTGCAAAATACTTTCGGATTATATGTTTTATCTTCTGGTGATGCAGCACAAGATGATGGCCCCATTCTTGGGAAATTGGCATATAGTCTTTCAGGACACTCGTGCAGAGGCAAAGAGGTTCTTTTCCAAGCACTCAATATCCGATCATCAAAACGCCTGCGACAGGATCACCGCCCTGAGGGCCAAATTCAGATCAGCAGCTGTGAAGGGAAGTAAAAGCAAATCTGTGTTGTTTGATGCATGTTTCCTAGCAGAACAGCTTGAAAATTTGGACGGCGACAAGTGGGAGCTAATGAGCGGAGTGTGGGTGGAATTGTTGTCCTATGCTTCCATCAAATGTAGACCAATTGTACATGCTCAGCAACCAAGTAGGGGTGGAGAACTTCTGACTTTCACTTGGCTACTGATGACTCATCTCGGCTTGGGAACACAATTCTATGAGCTCGAAAACCATGCTGGAAAAATGGTGCTAGTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

713

Amino Acids

81.53

Weight (kDa)

8.25

Isoelectric Point (pI)

43.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 52 - 401 2.8e-94 Domain of unknown function (DUF4220)
DUF594 PF04578 645 - 694 2.3e-18 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016402)

Species Orthologous Gene IDs
malus_domestica MD10G1305500.v1.1
prunus_persica Prupe.4G034200_v2.0.a1
pyrus_communis pycom10g25790
rosa_chinensis RchiOBHm_Chr3g0476871
rosa_laevigata RLG00000023747
rosa_multiflora Rmu_sc0000288.1_g000035
rosa_roxburghii Rroxscaffold_6G00404500
rosa_rugosa Rorug03G0157400
rosa_samantha Rh3AG207500 Rh3BG240100 Rh3CG233800 Rh3CG234900 Rh3DG233800
rosa_wichuraiana Rw0G011130 Rw3G018890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 947
AccBSI CCGCTC 1 cut(s) 1956
AccI GTMKAC 2 cut(s) 600, 1999
AccII CGCG 2 cut(s) 107, 895
AciI CCGC 5 cut(s) 105, 919, 1429, 1830, 1956
AclWI GGATC 5 cut(s) 421, 430, 434, 1183, 1832
AcsI RAATTY 3 cut(s) 1310, 1844, 1924
AcuI CTGAAG 2 cut(s) 951, 1419
AfaI GTAC 5 cut(s) 164, 457, 491, 525, 2010
AfiI CCNNNNNNNGG 7 cut(s) 542, 880, 1726, 1727, 1821, 1836, 2030
AflII CTTAAG 1 cut(s) 1111
AflIII ACRYGT 1 cut(s) 479
AgsI TTSAA 9 cut(s) 86, 359, 742, 775, 833, 1078, 1156, 1342, 1922
AhdI GACNNNNNGTC 1 cut(s) 607
AjnI CCWGG 3 cut(s) 375, 541, 1209
AloI GAACNNNNNNTCC 2 cut(s) 320, 352
Alw21I GWGCWC 3 cut(s) 852, 1326, 2103
Alw26I GTCTC 2 cut(s) 512, 554
AlwI GGATC 5 cut(s) 421, 430, 434, 1183, 1832
AlwNI CAGNNNCTG 2 cut(s) 548, 1859
AoxI GGCC 4 cut(s) 295, 620, 1716, 1839
ApeKI GCWGC 7 cut(s) 4, 185, 380, 383, 1345, 1702, 1856
ApoI RAATTY 3 cut(s) 1310, 1844, 1924
AseI ATTAAT 1 cut(s) 645
Asp700I GAANNNNTTC 1 cut(s) 1488
AspA2I CCTAGG 1 cut(s) 142
AspLEI GCGC 3 cut(s) 109, 789, 895
AspS9I GGNCC 6 cut(s) 296, 505, 545, 1098, 1717, 1839
AsuHPI GGTGA 3 cut(s) 1424, 1708, 1819
AsuII TTCGAA 1 cut(s) 732
AvaII GGWCC 3 cut(s) 505, 545, 1098
AvrII CCTAGG 1 cut(s) 142
AxyI CCTNAGG 1 cut(s) 1835
BamHI GGATCC 1 cut(s) 426
BanI GGYRCC 1 cut(s) 947
BanII GRGCYC 4 cut(s) 852, 1326, 1423, 2103
BauI CACGAG 1 cut(s) 1758
BbsI GAAGAC 4 cut(s) 255, 1425, 1570, 1654
Bbv12I GWGCWC 3 cut(s) 852, 1326, 2103
BbvCI CCTCAGC 1 cut(s) 1349
BbvI GCAGC 7 cut(s) 16, 172, 370, 392, 1357, 1714, 1868
BccI CCATC 5 cut(s) 665, 950, 1109, 1708, 1997
BceAI ACGGC 3 cut(s) 94, 592, 1948
BcgI CGANNNNNNTGC 8 cut(s) 164, 198, 1557, 1591, 1641, 1675, 2093, 2127
BciT130I CCWGG 3 cut(s) 377, 543, 1211
BcoDI GTCTC 2 cut(s) 512, 554
BfaI CTAG 5 cut(s) 143, 440, 1271, 1907, 2129
BfrI CTTAAG 1 cut(s) 1111
BglI GCCNNNNNGGC 3 cut(s) 115, 619, 1838
BisI GCNGC 7 cut(s) 5, 186, 381, 384, 1346, 1703, 1857
BlnI CCTAGG 1 cut(s) 142
BlpI GCTNAGC 2 cut(s) 851, 2016
BlsI GCNGC 7 cut(s) 6, 187, 382, 385, 1347, 1704, 1858
Bme1390I CCNGG 3 cut(s) 377, 543, 1211
Bme18I GGWCC 3 cut(s) 505, 545, 1098
BmeRI GACNNNNNGTC 1 cut(s) 607
BmgT120I GGNCC 6 cut(s) 296, 505, 545, 1098, 1717, 1839
BmiI GGNNCC 7 cut(s) 298, 428, 761, 949, 1045, 1127, 1719
BmrFI CCNGG 3 cut(s) 377, 543, 1211
BmrI ACTGGG 3 cut(s) 187, 1180, 1426
BmsI GCATC 7 cut(s) 50, 295, 1357, 1543, 1627, 1689, 1885
BmuI ACTGGG 3 cut(s) 187, 1180, 1426
BoxI GACNNNNGTC 1 cut(s) 407
BpiI GAAGAC 4 cut(s) 255, 1425, 1570, 1654
BpmI CTGGAG 4 cut(s) 344, 1144, 1409, 1477
Bpu10I CCTNAGC 2 cut(s) 344, 1349
Bpu1102I GCTNAGC 2 cut(s) 851, 2016
Bpu14I TTCGAA 1 cut(s) 732
BpuEI CTTGAG 3 cut(s) 91, 666, 1310
BsaI GGTCTC 1 cut(s) 554
BsaJI CCNNGG 4 cut(s) 142, 879, 1545, 1629
Bsc4I CCNNNNNNNGG 7 cut(s) 542, 880, 1726, 1727, 1821, 1836, 2030
Bse118I RCCGGY 1 cut(s) 452
Bse1I ACTGG 5 cut(s) 182, 1100, 1186, 1421, 1460
Bse21I CCTNAGG 1 cut(s) 1835
Bse3DI GCAATG 3 cut(s) 1031, 1104, 1503
BseBI CCWGG 3 cut(s) 377, 543, 1211
BseDI CCNNGG 4 cut(s) 142, 879, 1545, 1629
BseGI GGATG 6 cut(s) 46, 65, 363, 1219, 1324, 1372
BseLI CCNNNNNNNGG 7 cut(s) 542, 880, 1726, 1727, 1821, 1836, 2030
BseMI GCAATG 3 cut(s) 1031, 1104, 1503
BseMII CTCAG 8 cut(s) 44, 358, 758, 865, 1296, 1340, 1826, 2030
BseNI ACTGG 5 cut(s) 182, 1100, 1186, 1421, 1460
BseXI GCAGC 7 cut(s) 16, 172, 370, 392, 1357, 1714, 1868
BsgI GTGCAG 1 cut(s) 1782
Bsh1236I CGCG 2 cut(s) 107, 895
BshFI GGCC 4 cut(s) 297, 622, 1718, 1841
BshNI GGYRCC 1 cut(s) 947
BsiHKAI GWGCWC 3 cut(s) 852, 1326, 2103
BsiSI CCGG 1 cut(s) 453
BslFI GGGAC 2 cut(s) 238, 1168
BslI CCNNNNNNNGG 7 cut(s) 542, 880, 1726, 1727, 1821, 1836, 2030
BsmAI GTCTC 2 cut(s) 512, 554
BsmFI GGGAC 2 cut(s) 238, 1168
BsmI GAATGC 3 cut(s) 63, 260, 1232
BsnI GGCC 4 cut(s) 297, 622, 1718, 1841
Bso31I GGTCTC 1 cut(s) 554
Bsp119I TTCGAA 1 cut(s) 732
Bsp1286I GDGCHC 4 cut(s) 852, 1326, 1423, 2103
Bsp1407I TGTACA 1 cut(s) 2008
Bsp143I GATC 7 cut(s) 150, 426, 435, 1175, 1801, 1824, 1850
Bsp1720I GCTNAGC 2 cut(s) 851, 2016
BspACI CCGC 5 cut(s) 105, 919, 1429, 1830, 1956
BspANI GGCC 4 cut(s) 297, 622, 1718, 1841
BspCNI CTCAG 8 cut(s) 45, 357, 759, 864, 1297, 1341, 1827, 2029
BspFNI CGCG 2 cut(s) 107, 895
BspLI GGNNCC 7 cut(s) 298, 428, 761, 949, 1045, 1127, 1719
BspPI GGATC 5 cut(s) 421, 430, 434, 1183, 1832
BspT104I TTCGAA 1 cut(s) 732
BspT107I GGYRCC 1 cut(s) 947
BspTI CTTAAG 1 cut(s) 1111
BspTNI GGTCTC 1 cut(s) 554
BsrBI CCGCTC 1 cut(s) 1956
BsrDI GCAATG 3 cut(s) 1031, 1104, 1503
BsrFI RCCGGY 1 cut(s) 452
BsrGI TGTACA 1 cut(s) 2008
BsrI ACTGG 5 cut(s) 182, 1100, 1186, 1421, 1460
BssAI RCCGGY 1 cut(s) 452
BssECI CCNNGG 4 cut(s) 142, 879, 1545, 1629
BssMI GATC 7 cut(s) 150, 426, 435, 1175, 1801, 1824, 1850
BssSI CACGAG 1 cut(s) 1758
BssT1I CCWWGG 4 cut(s) 142, 879, 1545, 1629
Bst2BI CACGAG 1 cut(s) 1758
Bst2UI CCWGG 3 cut(s) 377, 543, 1211
Bst4CI ACNGT 3 cut(s) 523, 565, 1591
Bst6I CTCTTC 1 cut(s) 552
BstAFI CTTAAG 1 cut(s) 1111
BstAUI TGTACA 1 cut(s) 2008
BstBI TTCGAA 1 cut(s) 732
BstC8I GCNNGC 3 cut(s) 499, 1399, 1816
BstDEI CTNAG 8 cut(s) 53, 344, 767, 851, 1305, 1349, 1835, 2016
BstENI CCTNNNNNAGG 1 cut(s) 1819
BstF5I GGATG 6 cut(s) 46, 65, 363, 1219, 1324, 1372
BstFNI CGCG 2 cut(s) 107, 895
BstHHI GCGC 3 cut(s) 109, 789, 895
BstKTI GATC 7 cut(s) 153, 429, 438, 1178, 1804, 1827, 1853
BstMAI GTCTC 2 cut(s) 512, 554
BstMBI GATC 7 cut(s) 150, 426, 435, 1175, 1801, 1824, 1850
BstMWI GCNNNNNNNGC 6 cut(s) 106, 115, 266, 619, 1244, 1838
BstNI CCWGG 3 cut(s) 377, 543, 1211
BstNSI RCATGY 6 cut(s) 136, 483, 607, 1401, 1902, 2015
BstPAI GACNNNNGTC 1 cut(s) 407
BstSCI CCNGG 3 cut(s) 375, 541, 1209
BstUI CGCG 2 cut(s) 107, 895
BstV1I GCAGC 7 cut(s) 16, 172, 370, 392, 1357, 1714, 1868
BstV2I GAAGAC 4 cut(s) 255, 1425, 1570, 1654
BstX2I RGATCY 1 cut(s) 426
BstYI RGATCY 1 cut(s) 426
Bsu36I CCTNAGG 1 cut(s) 1835
BsuRI GGCC 4 cut(s) 297, 622, 1718, 1841
BtsCI GGATG 6 cut(s) 46, 65, 363, 1219, 1324, 1372
BtsIMutI CAGTG 1 cut(s) 829
Cac8I GCNNGC 3 cut(s) 499, 1399, 1816
CaiI CAGNNNCTG 2 cut(s) 548, 1859
CfoI GCGC 3 cut(s) 109, 789, 895
Cfr10I RCCGGY 1 cut(s) 452
Cfr13I GGNCC 6 cut(s) 296, 505, 545, 1098, 1717, 1839
CseI GACGC 1 cut(s) 1208
CsiI ACCWGGT 1 cut(s) 541
Csp6I GTAC 5 cut(s) 163, 456, 490, 524, 2009
CviQI GTAC 5 cut(s) 163, 456, 490, 524, 2009
DdeI CTNAG 8 cut(s) 53, 344, 767, 851, 1305, 1349, 1835, 2016
DpnI GATC 7 cut(s) 152, 428, 437, 1177, 1803, 1826, 1852
DpnII GATC 7 cut(s) 150, 426, 435, 1175, 1801, 1824, 1850
DriI GACNNNNNGTC 1 cut(s) 607
Eam1104I CTCTTC 1 cut(s) 552
Eam1105I GACNNNNNGTC 1 cut(s) 607
EarI CTCTTC 1 cut(s) 552
Ecl136II GAGCTC 3 cut(s) 850, 1324, 2101
Eco130I CCWWGG 4 cut(s) 142, 879, 1545, 1629
Eco24I GRGCYC 4 cut(s) 852, 1326, 1423, 2103
Eco31I GGTCTC 1 cut(s) 554
Eco47I GGWCC 3 cut(s) 505, 545, 1098
Eco53kI GAGCTC 3 cut(s) 850, 1324, 2101
Eco57I CTGAAG 2 cut(s) 951, 1419
Eco81I CCTNAGG 1 cut(s) 1835
EcoICRI GAGCTC 3 cut(s) 850, 1324, 2101
EcoNI CCTNNNNNAGG 1 cut(s) 1819
EcoO109I RGGNCCY 1 cut(s) 545
EcoRI GAATTC 1 cut(s) 1310
EcoRII CCWGG 3 cut(s) 375, 541, 1209
EcoT14I CCWWGG 4 cut(s) 142, 879, 1545, 1629
EcoT22I ATGCAT 3 cut(s) 65, 1033, 1900
EcoT38I GRGCYC 4 cut(s) 852, 1326, 1423, 2103
ErhI CCWWGG 4 cut(s) 142, 879, 1545, 1629
FalI AAGNNNNNCTT 2 cut(s) 1763, 1795
FaqI GGGAC 2 cut(s) 238, 1168
FblI GTMKAC 2 cut(s) 600, 1999
Fnu4HI GCNGC 7 cut(s) 5, 186, 381, 384, 1346, 1703, 1857
FokI GGATG 6 cut(s) 53, 72, 350, 1226, 1331, 1379
FriOI GRGCYC 4 cut(s) 852, 1326, 1423, 2103
Fsp4HI GCNGC 7 cut(s) 5, 186, 381, 384, 1346, 1703, 1857
FspBI CTAG 5 cut(s) 143, 440, 1271, 1907, 2129
GlaI GCGC 3 cut(s) 108, 788, 894
GluI GCNGC 7 cut(s) 5, 186, 381, 384, 1346, 1703, 1857
GsuI CTGGAG 4 cut(s) 344, 1144, 1409, 1477
HaeIII GGCC 4 cut(s) 297, 622, 1718, 1841
HapII CCGG 1 cut(s) 453
HgaI GACGC 1 cut(s) 1208
HhaI GCGC 3 cut(s) 109, 789, 895
Hin6I GCGC 3 cut(s) 107, 787, 893
HinP1I GCGC 3 cut(s) 107, 787, 893
HindIII AAGCTT 3 cut(s) 419, 499, 736
HinfI GANTC 7 cut(s) 49, 233, 686, 716, 747, 1266, 2068
HpaII CCGG 1 cut(s) 453
HphI GGTGA 3 cut(s) 1424, 1708, 1819
Hpy166II GTNNAC 2 cut(s) 601, 2000
Hpy188I TCNGA 9 cut(s) 54, 175, 768, 1093, 1306, 1675, 1801, 1850, 2046
Hpy8I GTNNAC 2 cut(s) 601, 2000
Hpy99I CGWCG 3 cut(s) 436, 569, 613
HpyAV CCTTC 5 cut(s) 25, 773, 940, 1158, 1858
HpyCH4III ACNGT 3 cut(s) 523, 565, 1591
HpyF10VI GCNNNNNNNGC 6 cut(s) 106, 115, 266, 619, 1244, 1838
HpyF3I CTNAG 8 cut(s) 53, 344, 767, 851, 1305, 1349, 1835, 2016
HspAI GCGC 3 cut(s) 107, 787, 893
Kzo9I GATC 7 cut(s) 150, 426, 435, 1175, 1801, 1824, 1850
LmnI GCTCC 7 cut(s) 144, 784, 921, 1043, 1125, 1244, 1945
Lsp1109I GCAGC 7 cut(s) 16, 172, 370, 392, 1357, 1714, 1868
LweI GCATC 7 cut(s) 50, 295, 1357, 1543, 1627, 1689, 1885
MabI ACCWGGT 1 cut(s) 541
MaeI CTAG 5 cut(s) 143, 440, 1271, 1907, 2129
MaeIII GTNAC 2 cut(s) 230, 1412
MalI GATC 7 cut(s) 152, 428, 437, 1177, 1803, 1826, 1852
MbiI CCGCTC 1 cut(s) 1956
MboI GATC 7 cut(s) 150, 426, 435, 1175, 1801, 1824, 1850
MfeI CAATTG 1 cut(s) 2004
MflI RGATCY 1 cut(s) 426
MhlI GDGCHC 4 cut(s) 852, 1326, 1423, 2103
MlyI GAGTC 3 cut(s) 227, 741, 2062
MmeI TCCRAC 2 cut(s) 198, 1021
Mph1103I ATGCAT 3 cut(s) 65, 1033, 1900
MroXI GAANNNNTTC 1 cut(s) 1488
MseI TTAA 4 cut(s) 645, 923, 987, 1112
MslI CAYNNNNRTG 2 cut(s) 291, 999
MspA1I CMGCKG 4 cut(s) 383, 1348, 1429, 1859
MspCI CTTAAG 1 cut(s) 1111
MspI CCGG 1 cut(s) 453
MspR9I CCNGG 3 cut(s) 377, 543, 1211
MunI CAATTG 1 cut(s) 2004
Mva1269I GAATGC 3 cut(s) 63, 260, 1232
MvaI CCWGG 3 cut(s) 377, 543, 1211
MvnI CGCG 2 cut(s) 107, 895
MwoI GCNNNNNNNGC 6 cut(s) 106, 115, 266, 619, 1244, 1838
NdeII GATC 7 cut(s) 150, 426, 435, 1175, 1801, 1824, 1850
NlaIV GGNNCC 7 cut(s) 298, 428, 761, 949, 1045, 1127, 1719
NmeAIII GCCGAG 1 cut(s) 2055
NmuCI GTSAC 2 cut(s) 230, 1412
NsiI ATGCAT 3 cut(s) 65, 1033, 1900
NspI RCATGY 6 cut(s) 136, 483, 607, 1401, 1902, 2015
NspV TTCGAA 1 cut(s) 732
PaeI GCATGC 1 cut(s) 1401
PciI ACATGT 1 cut(s) 479
PcsI WCGNNNNNNNCGW 1 cut(s) 969
PctI GAATGC 3 cut(s) 63, 260, 1232
PdmI GAANNNNTTC 1 cut(s) 1488
PfeI GAWTC 4 cut(s) 49, 686, 716, 1266
PkrI GCNGC 7 cut(s) 6, 187, 382, 385, 1347, 1704, 1858
PleI GAGTC 3 cut(s) 227, 741, 2062
PpsI GAGTC 3 cut(s) 227, 741, 2062
PpuMI RGGWCCY 1 cut(s) 545
PscI ACATGT 1 cut(s) 479
PshAI GACNNNNGTC 1 cut(s) 407
PshBI ATTAAT 1 cut(s) 645
Psp124BI GAGCTC 3 cut(s) 852, 1326, 2103
Psp5II RGGWCCY 1 cut(s) 545
Psp6I CCWGG 3 cut(s) 375, 541, 1209
PspGI CCWGG 3 cut(s) 375, 541, 1209
PspN4I GGNNCC 7 cut(s) 298, 428, 761, 949, 1045, 1127, 1719
PspPI GGNCC 6 cut(s) 296, 505, 545, 1098, 1717, 1839
PspPPI RGGWCCY 1 cut(s) 545
PstNI CAGNNNCTG 2 cut(s) 548, 1859
PsuI RGATCY 1 cut(s) 426
PvuII CAGCTG 3 cut(s) 383, 1348, 1859
RsaI GTAC 5 cut(s) 164, 457, 491, 525, 2010
RsaNI GTAC 5 cut(s) 163, 456, 490, 524, 2009
RseI CAYNNNNRTG 2 cut(s) 291, 999
SacI GAGCTC 3 cut(s) 852, 1326, 2103
SaqAI TTAA 4 cut(s) 645, 923, 987, 1112
SatI GCNGC 7 cut(s) 5, 186, 381, 384, 1346, 1703, 1857
Sau3AI GATC 7 cut(s) 150, 426, 435, 1175, 1801, 1824, 1850
Sau96I GGNCC 6 cut(s) 296, 505, 545, 1098, 1717, 1839
SchI GAGTC 3 cut(s) 227, 741, 2062
ScrFI CCNGG 3 cut(s) 377, 543, 1211
SduI GDGCHC 4 cut(s) 852, 1326, 1423, 2103
SexAI ACCWGGT 1 cut(s) 541
SfaNI GCATC 7 cut(s) 50, 295, 1357, 1543, 1627, 1689, 1885
SfuI TTCGAA 1 cut(s) 732
SinI GGWCC 3 cut(s) 505, 545, 1098
SmiMI CAYNNNNRTG 2 cut(s) 291, 999
SmlI CTYRAG 4 cut(s) 70, 681, 1111, 1325
SmoI CTYRAG 4 cut(s) 70, 681, 1111, 1325
SphI GCATGC 1 cut(s) 1401
SsiI CCGC 5 cut(s) 105, 919, 1429, 1830, 1956
SspMI CTAG 5 cut(s) 143, 440, 1271, 1907, 2129
SstI GAGCTC 3 cut(s) 852, 1326, 2103
StyD4I CCNGG 3 cut(s) 375, 541, 1209
StyI CCWWGG 4 cut(s) 142, 879, 1545, 1629
TaaI ACNGT 3 cut(s) 523, 565, 1591
TaqI TCGA 7 cut(s) 411, 567, 732, 793, 807, 1056, 2103
TatI WGTACW 2 cut(s) 523, 2008
TfiI GAWTC 4 cut(s) 49, 686, 716, 1266
Tru1I TTAA 4 cut(s) 645, 923, 987, 1112
Tru9I TTAA 4 cut(s) 645, 923, 987, 1112
TscAI CASTG 1 cut(s) 829
TseFI GTSAC 2 cut(s) 230, 1412
TseI GCWGC 7 cut(s) 4, 185, 380, 383, 1345, 1702, 1856
Tsp45I GTSAC 2 cut(s) 230, 1412
TspDTI ATGAA 6 cut(s) 44, 261, 261, 570, 847, 1278
TspGWI ACGGA 3 cut(s) 1331, 1575, 1659
TspRI CASTG 1 cut(s) 829
Vha464I CTTAAG 1 cut(s) 1111
VpaK11BI GGWCC 3 cut(s) 505, 545, 1098
VspI ATTAAT 1 cut(s) 645
XagI CCTNNNNNAGG 1 cut(s) 1819
XapI RAATTY 3 cut(s) 1310, 1844, 1924
XceI RCATGY 6 cut(s) 136, 483, 607, 1401, 1902, 2015
XcmI CCANNNNNNNNNTGG 1 cut(s) 886
XmaJI CCTAGG 1 cut(s) 142
XmiI GTMKAC 2 cut(s) 600, 1999
XmnI GAANNNNTTC 1 cut(s) 1488
XspI CTAG 5 cut(s) 143, 440, 1271, 1907, 2129
Zsp2I ATGCAT 3 cut(s) 65, 1033, 1900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.