Prupe.4G034200_v2.0.a1

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0016402
Physical Location & Seq
Forward (+)
1590886 .. 1593797
2912 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G034200.1

Sequence Viewer

Length: 2034 bp
ATGAAGCTACCTATACCGAAGGAGGTCTTGAAGATATGGGATGAATGGAATCTGAGGGGCTGTATTCTCTTGAGCCTTGCACTTCAAATTTTCTTATTTTTTTTTGCACCACTCAGGCAAAAATGCAGAAGCAAATTGATTGTAGGGTTGATCTGGTTGGCGTACTTGCTCTCCGACTGGGTTGCTGCGGTTGCAATTGGGGTAATCACCAAAAGCCAGGGTAACCCTTGTGACTCTGGAAAGAATAAAGACCTTTTAGCATTCTGGGCTTCATTTCTTTTAGTGCATCTTGGTGGCCCTGATACTATTACTTCTTTTGCTCTAGAGGACAATGAGTTCTGGCTCAGGCATTTGATTCAACTCATTCTACAAGTCCTGGCAGCTGCTTACAGCTTCTATATGACACTTTTAGAAAACAAGCTTTGGTTCCCAACAGTCCTAGTGTTTGTTGTGGGAACTATCAAGTTTGGGGAGAGAACATGTGCTCTGTATCTTGCTAGCTTGGACCATTTTGGAGAGATTGTATTGCCAGAGCCCGAGCCTGGGCCTGACTATGAAGAGGCTGTGGAAATATACTCTACAATGAGGTCAGTCGAAGTTCCAACACAAGTAGAGCTGCCAATAAGACCAATGCACATTGGGAATTATAGGAACCCCAAATTTACAGTTGAAGGAGTTCTTAAAGAATCTCTAGATGACGTTCAGTTACTGCAGGAAGCATATCGCTTCTTTGAAAGTTTCAAAGGGCTCATTGTTGGTTTCCTTCTGAGCTCCAAAGTTCGAGAACGCAGCCGAGATTTTTTTCTCAAAAGAACCCATGTTAGTGCTTTCCAATTGATAGAGTATGAGCTCAGCTTCATGTATGAGGTTCTCCATACCAAGGTGGTTGTGGCTCGTCACAAAGTTGGGTACATTCTCCGCTTACTTACCTTCTGTTCAACAATTGGTGCCTTGATGTTATTTGCTTTAGTTGGAAAACATAAGTTTGATACGTTTGACATTGCGCTTACCTATGCTTTGCTTATTGGGGCCATAATCCTGGACACTCTGTCTGTGTTGAATCTCATTTGTTCAGACTGGACAGTCATTGCCTTCAACAACATATGGAGCAGATCATATATTGGTGCATTGATATTGAAACTCAAAAGGGGAAGGTGGTCTGGATCGGTGTCCAAGTATAATATGATAAGCAATTGCCTGGATGAGCATCCAGAATGGGTGTACACTTTTGCTGGTTATATGGGCGTTAGAGGAATTCTAGATAAAATAAAAGTCTGTTTTTTTTCAACCTCAGAGGCAGTCACTGAAGATGTCAAGAAATTTTTGTTCAAGCAGCTGAGGATAAAATCTTTGGAAGCAAACAACAGAAGAGCTGCCATGGAGGCATCTTCACAAAGAGGTGACTGGGCTCTCCTGCAGACTTCAAACTATCTGGAACTCAAATGGAGTGTAGGGGAATGTCAATATGCAGAAAGTGTTCTTCTGTGGCACATAGCAACGGAACTCTACTTCGCGAAAAAGAAGCCCAACGCTGATCAGGAAAGAAGAGAAATTTGCAAACTACTTTCAGATTATATGTTCTATCTTCTGGTGATGCAGCATAATATGATGGCCCCATTTTTGGGGAATTGGCATATAGTTTTCCAGGACACACGTGCAGAGGCCAAGAGGTTCTTTCTCAAGCACAAAATAACTGATCATGGGAAAGCTTGTGAAAAGATTAATGCTGTGGAAGCCAAATTCAGATCAGCCGCTGTGAAGGGTAAAAGCAAATCTGTGTTGTTCGATGCATGTTTCCTAGTAAAACAGCTTGAAGAGGTCGAGGAAGACCCATGGAAGCTAATGAGCCGAGTGTGGGTGGAATTATTGTCCTATGCTTCCATTAAATGTAGACCAATTGTGCATGCTCAGCAACCAAGTAGGGGTGGAGAACTTCTGACTTTCACTTGGCTACTGATGAATCATCTGGGCTTGGGAACACAATTCTATGAGCTGGAAAACCAAGCTGGAACCAAAATGGTGGCAGTTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

678

Amino Acids

77.72

Weight (kDa)

8.65

Isoelectric Point (pI)

40.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016402)

Species Orthologous Gene IDs
malus_domestica MD10G1305500.v1.1
prunus_persica Prupe.4G034200_v2.0.a1
pyrus_communis pycom10g25790
rosa_chinensis RchiOBHm_Chr3g0476871
rosa_laevigata RLG00000023747
rosa_multiflora Rmu_sc0000288.1_g000035
rosa_roxburghii Rroxscaffold_6G00404500
rosa_rugosa Rorug03G0157400
rosa_samantha Rh3AG207500 Rh3BG240100 Rh3CG233800 Rh3CG234900 Rh3DG233800
rosa_wichuraiana Rw0G011130 Rw3G018890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1082
AccB1I GGYRCC 1 cut(s) 947
AccI GTMKAC 1 cut(s) 1891
AccII CGCG 1 cut(s) 1514
AciI CCGC 3 cut(s) 188, 919, 1752
AclWI GGATC 1 cut(s) 1171
AcsI RAATTY 6 cut(s) 87, 659, 1254, 1319, 1551, 1739
AcuI CTGAAG 1 cut(s) 1326
AcvI CACGTG 1 cut(s) 1655
AfaI GTAC 3 cut(s) 164, 911, 1223
AfiI CCNNNNNNNGG 7 cut(s) 542, 543, 880, 1621, 1622, 1855, 1922
AflIII ACRYGT 2 cut(s) 479, 1652
AhdI GACNNNNNGTC 1 cut(s) 1048
AjnI CCWGG 6 cut(s) 216, 375, 541, 1038, 1197, 1644
AloI GAACNNNNNNTCC 2 cut(s) 320, 352
Alw21I GWGCWC 3 cut(s) 487, 773, 852
AlwI GGATC 1 cut(s) 1171
AlwNI CAGNNNCTG 3 cut(s) 709, 1304, 1754
Ama87I CYCGRG 1 cut(s) 536
AoxI GGCC 5 cut(s) 295, 545, 1029, 1611, 1662
ApeKI GCWGC 8 cut(s) 185, 380, 383, 616, 789, 1333, 1373, 1597
ApoI RAATTY 6 cut(s) 87, 659, 1254, 1319, 1551, 1739
AseI ATTAAT 1 cut(s) 1722
Asp700I GAANNNNTTC 2 cut(s) 675, 1476
AspLEI GCGC 1 cut(s) 1006
AspS9I GGNCC 5 cut(s) 296, 505, 545, 1029, 1612
AsuHPI GGTGA 3 cut(s) 199, 1412, 1603
AsuNHI GCTAGC 1 cut(s) 497
AvaI CYCGRG 1 cut(s) 536
AvaII GGWCC 1 cut(s) 505
BanI GGYRCC 1 cut(s) 947
BanII GRGCYC 5 cut(s) 537, 750, 773, 852, 1411
BbrPI CACGTG 1 cut(s) 1655
BbsI GAAGAC 1 cut(s) 1833
Bbv12I GWGCWC 3 cut(s) 487, 773, 852
BbvCI CCTCAGC 1 cut(s) 1337
BbvI GCAGC 8 cut(s) 172, 370, 392, 603, 801, 1345, 1360, 1609
BccI CCATC 1 cut(s) 1603
BcgI CGANNNNNNTGC 2 cut(s) 164, 198
BciT130I CCWGG 6 cut(s) 218, 377, 543, 1040, 1199, 1646
BclI TGATCA 2 cut(s) 1534, 1696
BfaI CTAG 6 cut(s) 323, 440, 498, 692, 1259, 1799
BfmI CTRYAG 2 cut(s) 710, 1415
BglI GCCNNNNNGGC 1 cut(s) 1382
BisI GCNGC 9 cut(s) 186, 381, 384, 617, 790, 1334, 1374, 1598, 1752
BlpI GCTNAGC 2 cut(s) 851, 1908
BlsI GCNGC 9 cut(s) 187, 382, 385, 618, 791, 1335, 1375, 1599, 1753
Bme1390I CCNGG 6 cut(s) 218, 377, 543, 1040, 1199, 1646
Bme18I GGWCC 1 cut(s) 505
BmeRI GACNNNNNGTC 1 cut(s) 1048
BmeT110I CYCGRG 1 cut(s) 536
BmgT120I GGNCC 5 cut(s) 296, 505, 545, 1029, 1612
BmiI GGNNCC 6 cut(s) 428, 653, 949, 1030, 1614, 2011
BmrFI CCNGG 6 cut(s) 218, 377, 543, 1040, 1199, 1646
BmrI ACTGGG 2 cut(s) 187, 1414
BmsI GCATC 5 cut(s) 295, 1216, 1394, 1584, 1777
BmtI GCTAGC 1 cut(s) 501
BmuI ACTGGG 2 cut(s) 187, 1414
BpiI GAAGAC 1 cut(s) 1833
Bpu10I CCTNAGC 2 cut(s) 344, 1337
Bpu1102I GCTNAGC 2 cut(s) 851, 1908
BpuEI CTTGAG 2 cut(s) 91, 1664
BsaAI YACGTR 1 cut(s) 1655
BsaBI GATNNNNATC 1 cut(s) 1206
BsaJI CCNNGG 5 cut(s) 217, 542, 879, 1377, 1832
BsaXI ACNNNNNCTCC 4 cut(s) 155, 185, 507, 537
Bsc4I CCNNNNNNNGG 7 cut(s) 542, 543, 880, 1621, 1622, 1855, 1922
Bse1I ACTGG 3 cut(s) 182, 1082, 1409
Bse3DI GCAATG 2 cut(s) 999, 1086
Bse8I GATNNNNATC 1 cut(s) 1206
BseBI CCWGG 6 cut(s) 218, 377, 543, 1040, 1199, 1646
BseDI CCNNGG 5 cut(s) 217, 542, 879, 1377, 1832
BseGI GGATG 3 cut(s) 46, 1207, 1207
BseJI GATNNNNATC 1 cut(s) 1206
BseLI CCNNNNNNNGG 7 cut(s) 542, 543, 880, 1621, 1622, 1855, 1922
BseMI GCAATG 2 cut(s) 999, 1086
BseMII CTCAG 8 cut(s) 44, 127, 358, 758, 865, 1305, 1328, 1922
BseNI ACTGG 3 cut(s) 182, 1082, 1409
BseXI GCAGC 8 cut(s) 172, 370, 392, 603, 801, 1345, 1360, 1609
BsgI GTGCAG 1 cut(s) 1677
Bsh1236I CGCG 1 cut(s) 1514
BshFI GGCC 5 cut(s) 297, 547, 1031, 1613, 1664
BshNI GGYRCC 1 cut(s) 947
BsiHKAI GWGCWC 3 cut(s) 487, 773, 852
BsiHKCI CYCGRG 1 cut(s) 536
BslI CCNNNNNNNGG 7 cut(s) 542, 543, 880, 1621, 1622, 1855, 1922
BsmI GAATGC 1 cut(s) 260
BsnI GGCC 5 cut(s) 297, 547, 1031, 1613, 1664
BsoBI CYCGRG 1 cut(s) 536
Bsp1286I GDGCHC 6 cut(s) 487, 537, 750, 773, 852, 1411
Bsp1407I TGTACA 1 cut(s) 1221
Bsp143I GATC 6 cut(s) 150, 1112, 1163, 1534, 1696, 1745
Bsp1720I GCTNAGC 2 cut(s) 851, 1908
Bsp19I CCATGG 2 cut(s) 1377, 1832
Bsp68I TCGCGA 1 cut(s) 1514
BspACI CCGC 3 cut(s) 188, 919, 1752
BspANI GGCC 5 cut(s) 297, 547, 1031, 1613, 1664
BspCNI CTCAG 8 cut(s) 45, 126, 357, 759, 864, 1304, 1329, 1921
BspFNI CGCG 1 cut(s) 1514
BspLI GGNNCC 6 cut(s) 428, 653, 949, 1030, 1614, 2011
BspMAI CTGCAG 2 cut(s) 714, 1419
BspOI GCTAGC 1 cut(s) 501
BspPI GGATC 1 cut(s) 1171
BspQI GCTCTTC 1 cut(s) 1363
BspT107I GGYRCC 1 cut(s) 947
BsrDI GCAATG 2 cut(s) 999, 1086
BsrGI TGTACA 1 cut(s) 1221
BsrI ACTGG 3 cut(s) 182, 1082, 1409
BssECI CCNNGG 5 cut(s) 217, 542, 879, 1377, 1832
BssMI GATC 6 cut(s) 150, 1112, 1163, 1534, 1696, 1745
BssT1I CCWWGG 3 cut(s) 879, 1377, 1832
Bst2UI CCWGG 6 cut(s) 218, 377, 543, 1040, 1199, 1646
Bst4CI ACNGT 3 cut(s) 436, 667, 1084
Bst6I CTCTTC 4 cut(s) 552, 1363, 1540, 1809
BstAUI TGTACA 1 cut(s) 1221
BstBAI YACGTR 1 cut(s) 1655
BstC8I GCNNGC 2 cut(s) 499, 1905
BstDEI CTNAG 8 cut(s) 53, 113, 344, 767, 851, 1291, 1337, 1908
BstDSI CCRYGG 2 cut(s) 1377, 1832
BstEII GGTNACC 1 cut(s) 221
BstF5I GGATG 3 cut(s) 46, 1207, 1207
BstFNI CGCG 1 cut(s) 1514
BstHHI GCGC 1 cut(s) 1006
BstKTI GATC 6 cut(s) 153, 1115, 1166, 1537, 1699, 1748
BstMBI GATC 6 cut(s) 150, 1112, 1163, 1534, 1696, 1745
BstMWI GCNNNNNNNGC 5 cut(s) 191, 266, 1382, 1733, 1909
BstNI CCWGG 6 cut(s) 218, 377, 543, 1040, 1199, 1646
BstNSI RCATGY 3 cut(s) 483, 1794, 1907
BstPI GGTNACC 1 cut(s) 221
BstSCI CCNGG 6 cut(s) 216, 375, 541, 1038, 1197, 1644
BstSFI CTRYAG 2 cut(s) 710, 1415
BstUI CGCG 1 cut(s) 1514
BstV1I GCAGC 8 cut(s) 172, 370, 392, 603, 801, 1345, 1360, 1609
BstV2I GAAGAC 1 cut(s) 1833
BstXI CCANNNNNNTGG 2 cut(s) 1039, 2020
BsuRI GGCC 5 cut(s) 297, 547, 1031, 1613, 1664
BtgI CCRYGG 2 cut(s) 1377, 1832
BtsCI GGATG 3 cut(s) 46, 1207, 1207
BtsIMutI CAGTG 1 cut(s) 1302
BtuMI TCGCGA 1 cut(s) 1514
Cac8I GCNNGC 2 cut(s) 499, 1905
CaiI CAGNNNCTG 3 cut(s) 709, 1304, 1754
CfoI GCGC 1 cut(s) 1006
Cfr13I GGNCC 5 cut(s) 296, 505, 545, 1029, 1612
Csp6I GTAC 3 cut(s) 163, 910, 1222
CviAII CATG 8 cut(s) 480, 818, 859, 1378, 1700, 1791, 1833, 1904
CviQI GTAC 3 cut(s) 163, 910, 1222
DdeI CTNAG 8 cut(s) 53, 113, 344, 767, 851, 1291, 1337, 1908
DpnI GATC 6 cut(s) 152, 1114, 1165, 1536, 1698, 1747
DpnII GATC 6 cut(s) 150, 1112, 1163, 1534, 1696, 1745
DrdI GACNNNNNNGTC 1 cut(s) 1082
DriI GACNNNNNGTC 1 cut(s) 1048
DseDI GACNNNNNNGTC 1 cut(s) 1082
Eam1104I CTCTTC 4 cut(s) 552, 1363, 1540, 1809
Eam1105I GACNNNNNGTC 1 cut(s) 1048
EarI CTCTTC 4 cut(s) 552, 1363, 1540, 1809
Ecl136II GAGCTC 2 cut(s) 771, 850
Eco130I CCWWGG 3 cut(s) 879, 1377, 1832
Eco24I GRGCYC 5 cut(s) 537, 750, 773, 852, 1411
Eco47I GGWCC 1 cut(s) 505
Eco53kI GAGCTC 2 cut(s) 771, 850
Eco57I CTGAAG 1 cut(s) 1326
Eco72I CACGTG 1 cut(s) 1655
Eco88I CYCGRG 1 cut(s) 536
Eco91I GGTNACC 1 cut(s) 221
EcoICRI GAGCTC 2 cut(s) 771, 850
EcoO65I GGTNACC 1 cut(s) 221
EcoRI GAATTC 1 cut(s) 1254
EcoRII CCWGG 6 cut(s) 216, 375, 541, 1038, 1197, 1644
EcoT14I CCWWGG 3 cut(s) 879, 1377, 1832
EcoT22I ATGCAT 1 cut(s) 1792
EcoT38I GRGCYC 5 cut(s) 537, 750, 773, 852, 1411
ErhI CCWWGG 3 cut(s) 879, 1377, 1832
FaeI CATG 8 cut(s) 483, 821, 862, 1381, 1703, 1794, 1836, 1907
FalI AAGNNNNNCTT 6 cut(s) 11, 43, 663, 695, 1658, 1690
FatI CATG 8 cut(s) 479, 817, 858, 1377, 1699, 1790, 1832, 1903
FauNDI CATATG 1 cut(s) 1103
FbaI TGATCA 2 cut(s) 1534, 1696
FblI GTMKAC 1 cut(s) 1891
Fnu4HI GCNGC 9 cut(s) 186, 381, 384, 617, 790, 1334, 1374, 1598, 1752
FokI GGATG 3 cut(s) 53, 1194, 1214
FriOI GRGCYC 5 cut(s) 537, 750, 773, 852, 1411
Fsp4HI GCNGC 9 cut(s) 186, 381, 384, 617, 790, 1334, 1374, 1598, 1752
FspBI CTAG 6 cut(s) 323, 440, 498, 692, 1259, 1799
GlaI GCGC 1 cut(s) 1005
GluI GCNGC 9 cut(s) 186, 381, 384, 617, 790, 1334, 1374, 1598, 1752
HaeIII GGCC 5 cut(s) 297, 547, 1031, 1613, 1664
HhaI GCGC 1 cut(s) 1006
Hin1II CATG 8 cut(s) 483, 821, 862, 1381, 1703, 1794, 1836, 1907
Hin6I GCGC 1 cut(s) 1004
HinP1I GCGC 1 cut(s) 1004
HindIII AAGCTT 2 cut(s) 419, 1707
HinfI GANTC 6 cut(s) 49, 233, 355, 686, 1060, 1960
HphI GGTGA 3 cut(s) 199, 1412, 1603
Hpy166II GTNNAC 3 cut(s) 1222, 1224, 1892
Hpy188I TCNGA 8 cut(s) 54, 175, 768, 1075, 1294, 1570, 1745, 1938
Hpy8I GTNNAC 3 cut(s) 1222, 1224, 1892
HpyAV CCTTC 7 cut(s) 13, 665, 773, 940, 1102, 1146, 1753
HpyCH4III ACNGT 3 cut(s) 436, 667, 1084
HpyCH4IV ACGT 3 cut(s) 699, 992, 1654
HpyF10VI GCNNNNNNNGC 5 cut(s) 191, 266, 1382, 1733, 1909
HpyF3I CTNAG 8 cut(s) 53, 113, 344, 767, 851, 1291, 1337, 1908
HpySE526I ACGT 3 cut(s) 699, 992, 1654
Hsp92II CATG 8 cut(s) 483, 821, 862, 1381, 1703, 1794, 1836, 1907
HspAI GCGC 1 cut(s) 1004
Ksp22I TGATCA 2 cut(s) 1534, 1696
Kzo9I GATC 6 cut(s) 150, 1112, 1163, 1534, 1696, 1745
LguI GCTCTTC 1 cut(s) 1363
LmnI GCTCC 2 cut(s) 776, 1107
Lsp1109I GCAGC 8 cut(s) 172, 370, 392, 603, 801, 1345, 1360, 1609
LweI GCATC 5 cut(s) 295, 1216, 1394, 1584, 1777
MaeI CTAG 6 cut(s) 323, 440, 498, 692, 1259, 1799
MaeII ACGT 3 cut(s) 699, 992, 1654
MaeIII GTNAC 6 cut(s) 221, 230, 705, 896, 1300, 1400
MalI GATC 6 cut(s) 152, 1114, 1165, 1536, 1698, 1747
MboI GATC 6 cut(s) 150, 1112, 1163, 1534, 1696, 1745
MfeI CAATTG 5 cut(s) 195, 833, 942, 1192, 1896
MhlI GDGCHC 6 cut(s) 487, 537, 750, 773, 852, 1411
MlyI GAGTC 1 cut(s) 227
MmeI TCCRAC 3 cut(s) 198, 626, 952
Mph1103I ATGCAT 1 cut(s) 1792
MroXI GAANNNNTTC 2 cut(s) 675, 1476
MseI TTAA 4 cut(s) 681, 1722, 1884, 2028
MslI CAYNNNNRTG 2 cut(s) 291, 822
MspA1I CMGCKG 3 cut(s) 383, 1336, 1754
MspR9I CCNGG 6 cut(s) 218, 377, 543, 1040, 1199, 1646
MunI CAATTG 5 cut(s) 195, 833, 942, 1192, 1896
Mva1269I GAATGC 1 cut(s) 260
MvaI CCWGG 6 cut(s) 218, 377, 543, 1040, 1199, 1646
MvnI CGCG 1 cut(s) 1514
MwoI GCNNNNNNNGC 5 cut(s) 191, 266, 1382, 1733, 1909
NcoI CCATGG 2 cut(s) 1377, 1832
NdeI CATATG 1 cut(s) 1103
NdeII GATC 6 cut(s) 150, 1112, 1163, 1534, 1696, 1745
NheI GCTAGC 1 cut(s) 497
NlaIII CATG 8 cut(s) 483, 821, 862, 1381, 1703, 1794, 1836, 1907
NlaIV GGNNCC 6 cut(s) 428, 653, 949, 1030, 1614, 2011
NmeAIII GCCGAG 2 cut(s) 818, 1874
NmuCI GTSAC 4 cut(s) 230, 896, 1300, 1400
NruI TCGCGA 1 cut(s) 1514
NsiI ATGCAT 1 cut(s) 1792
NspI RCATGY 3 cut(s) 483, 1794, 1907
PaeI GCATGC 1 cut(s) 1907
PciI ACATGT 1 cut(s) 479
PciSI GCTCTTC 1 cut(s) 1363
PctI GAATGC 1 cut(s) 260
PdmI GAANNNNTTC 2 cut(s) 675, 1476
PfeI GAWTC 5 cut(s) 49, 355, 686, 1060, 1960
PfoI TCCNGGA 2 cut(s) 1038, 1644
PkrI GCNGC 9 cut(s) 187, 382, 385, 618, 791, 1335, 1375, 1599, 1753
PleI GAGTC 1 cut(s) 227
PmaCI CACGTG 1 cut(s) 1655
PmlI CACGTG 1 cut(s) 1655
PpsI GAGTC 1 cut(s) 227
Ppu21I YACGTR 1 cut(s) 1655
PscI ACATGT 1 cut(s) 479
PshBI ATTAAT 1 cut(s) 1722
Psp124BI GAGCTC 2 cut(s) 773, 852
Psp6I CCWGG 6 cut(s) 216, 375, 541, 1038, 1197, 1644
PspCI CACGTG 1 cut(s) 1655
PspEI GGTNACC 1 cut(s) 221
PspGI CCWGG 6 cut(s) 216, 375, 541, 1038, 1197, 1644
PspN4I GGNNCC 6 cut(s) 428, 653, 949, 1030, 1614, 2011
PspPI GGNCC 5 cut(s) 296, 505, 545, 1029, 1612
PstI CTGCAG 2 cut(s) 714, 1419
PstNI CAGNNNCTG 3 cut(s) 709, 1304, 1754
PvuII CAGCTG 2 cut(s) 383, 1336
RruI TCGCGA 1 cut(s) 1514
RsaI GTAC 3 cut(s) 164, 911, 1223
RsaNI GTAC 3 cut(s) 163, 910, 1222
RseI CAYNNNNRTG 2 cut(s) 291, 822
SacI GAGCTC 2 cut(s) 773, 852
SapI GCTCTTC 1 cut(s) 1363
SaqAI TTAA 4 cut(s) 681, 1722, 1884, 2028
SatI GCNGC 9 cut(s) 186, 381, 384, 617, 790, 1334, 1374, 1598, 1752
Sau3AI GATC 6 cut(s) 150, 1112, 1163, 1534, 1696, 1745
Sau96I GGNCC 5 cut(s) 296, 505, 545, 1029, 1612
SchI GAGTC 1 cut(s) 227
ScrFI CCNGG 6 cut(s) 218, 377, 543, 1040, 1199, 1646
SduI GDGCHC 6 cut(s) 487, 537, 750, 773, 852, 1411
SfaNI GCATC 5 cut(s) 295, 1216, 1394, 1584, 1777
SfcI CTRYAG 2 cut(s) 710, 1415
SinI GGWCC 1 cut(s) 505
SmiMI CAYNNNNRTG 2 cut(s) 291, 822
SmlI CTYRAG 2 cut(s) 70, 1679
SmoI CTYRAG 2 cut(s) 70, 1679
SphI GCATGC 1 cut(s) 1907
SsiI CCGC 3 cut(s) 188, 919, 1752
SspMI CTAG 6 cut(s) 323, 440, 498, 692, 1259, 1799
SstI GAGCTC 2 cut(s) 773, 852
StyD4I CCNGG 6 cut(s) 216, 375, 541, 1038, 1197, 1644
StyI CCWWGG 3 cut(s) 879, 1377, 1832
TaaI ACNGT 3 cut(s) 436, 667, 1084
TaiI ACGT 3 cut(s) 702, 995, 1657
TaqI TCGA 4 cut(s) 594, 781, 1785, 1821
TatI WGTACW 1 cut(s) 1221
TauI GCSGC 1 cut(s) 1754
TfiI GAWTC 5 cut(s) 49, 355, 686, 1060, 1960
Tru1I TTAA 4 cut(s) 681, 1722, 1884, 2028
Tru9I TTAA 4 cut(s) 681, 1722, 1884, 2028
TscAI CASTG 1 cut(s) 1309
TseFI GTSAC 4 cut(s) 230, 896, 1300, 1400
TseI GCWGC 8 cut(s) 185, 380, 383, 616, 789, 1333, 1373, 1597
Tsp45I GTSAC 4 cut(s) 230, 896, 1300, 1400
TspDTI ATGAA 6 cut(s) 17, 57, 261, 570, 847, 1973
TspGWI ACGGA 1 cut(s) 1514
TspRI CASTG 1 cut(s) 1309
VpaK11BI GGWCC 1 cut(s) 505
VspI ATTAAT 1 cut(s) 1722
XapI RAATTY 6 cut(s) 87, 659, 1254, 1319, 1551, 1739
XbaI TCTAGA 3 cut(s) 322, 691, 1258
XceI RCATGY 3 cut(s) 483, 1794, 1907
XcmI CCANNNNNNNNNTGG 1 cut(s) 886
XmiI GTMKAC 1 cut(s) 1891
XmnI GAANNNNTTC 2 cut(s) 675, 1476
XspI CTAG 6 cut(s) 323, 440, 498, 692, 1259, 1799
Zsp2I ATGCAT 1 cut(s) 1792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.