pycom10g25790

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0016402
Physical Location & Seq
Reverse (-)
27303816 .. 27305889
2074 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g25790.2

Sequence Viewer

Length: 2016 bp
ATGGTGAGAACTATGCAGCTACCTATACCCAAAGAGCTGATGAATATATGGGATGTATGGAATCTGAGGGGCTGCATTCTCTTGAGCCTTGCTGTTCAAGTTTTCCTAACGCTTTCTGCGCCTTTCAGGCAACAATGTAGAAGCAAGTTGCTCCTAGGATCGATCATCGCCAAAAGCCAGACTGAGATTTGTGGCTCTAGAAATAATGAAGACCTTTTAGCATTGTGGGCTTCGTTTCTTTTGGTGCATCTTGGTGGCCCTGATACCATTACTTCTTTTGCACTGGAGGACAATGAGTTCTGGCTCAGGCACTTGATTCAACTCATCCTACAAGTCCTGGCTGCTGCGTATAGTTTCTATATGACACTTGTCGAAAACAGGCTTTGGATCCCGACGATCCTAGTATTTGTTGCTGGAACTGTCAAGTTTGGGGAGAGAACATGCTGCCTGTATCTTGCAAGCTTGGACCATTTTGGAGAGACTGTATTGCCAAAGCCAGAACCTGGTCCTGACTATGAAGAGGCCGTCGACATATATTCTACAATGAGGTCAGTCAAAGTAGAGATGCCGCCAACAAGGCCATTGCACACTGTGAAAGGGATCAATCATAAGAAAAACCCCAAATTTACAGTTGACGGAGTTACTCAAGAATCTTTAAATGACATGCAGTTATTGCGGGAATCATATCACTTCTTCGAAAGCTTCAAAGGACTCATTGTTGGGTTCCTTCTGAGCTCCAAAGATCGAGAGCGCAGTCGAGATTTTTTTCTCAAAAGAACACCCATCAGTGCTTTTCAACTGATAGAGTACGAGCTCAGCTTCATGTATGAGGTTCTCCATACCAAGGCAGTTGTGGCGCGACACAAAATCGGCTACCTGCTCCGCTTGTTCACCTTCTGTTCAACCATCGGTGCCTTGATTTTGTTCATTTTAGTTGAAAAAGATGAGTTTGAGAAACATGAGTTTGGTAAGTTTGACATTGTTCTTACTTTTGTATTGCTTGGCGCAGCCATAGTCCTCGACATACTATCTGTGTTGTTGAAGCTCATTTTCTCTGACTGGACCATCATTGCCTTAAGAAACATCTGGAGCCAACGTTATATCCCTTCATGGATATTGAAAAGACGAAGGTGGTCTCGATCGGTGTCCCAGTATAATATGATAAGGAATTGCCTGGATGAGCGTCCAGAATGGGTGTTCACTATTGGTGGTTATGTGGGTGTTAGAGGAATCCTAGACAAAATAAAAGTTTCTCTCTTTTCTTCCTCTGAGAAATTCACGGATGAGCTCAAGGGCTTCATGTTCAAGCAGCTGAGGTTAAAATCTTTGGAAGCAAACAACAAAAGAACCGCCATGGAGGCATGCTCACAAAGAGGTGACTGGGCTCTCCTGCTGACTTCAGACTACGTGAAATTCAAATGGAGTGTAGGGGAATATCAGTATGCAGAAAGTGTTCTTCTGTGGCACATTGCAACAGAACTCTACTTTGCAAAAATCTTGGAAGATGCTGACGAAAGGCAAAAAATCTGCAAAATACTTTCGGATTATATGTTTTATCTTCTGGTGATGCAGCACAAGATGATGGCCCCATTCTTGGGGAATTGGCATATAGTCTTTCAGGACACTTGTGCAGAGGCAAAGAGATTCTTTTCCAAGCACTCAATATCTGATCATCGAAAAGCCTGCGCCGAGATCACTGGTGTGAAGGCCAAATTCAGATCAGCTGCTGTGAAGGGAAGTAAAAGCAAATCTGTGTTGTTTGATGCATGTTTCCTAGCAGGAGAGCTAGGAGAGCTTGCAAAGTTGGGACGAGACCCGTGGAAGCTAATGAGCCGAGTCTGGGTGGAATTGCTGTCCTATGCTTCCATCAAATGTAGACCAATTGTACATGCTCAGCAACCAAGTAGGGGTGGAGAACTTCTGACTTTCACTTGGCTACTGATGTATCATCTCGGCTTGGGAACACAATTCTATGAGCTCGAAAACCATGCTGAAAAAATGGTGCCAACCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

672

Amino Acids

77.42

Weight (kDa)

8.97

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016402)

Species Orthologous Gene IDs
malus_domestica MD10G1305500.v1.1
prunus_persica Prupe.4G034200_v2.0.a1
pyrus_communis pycom10g25790
rosa_chinensis RchiOBHm_Chr3g0476871
rosa_laevigata RLG00000023747
rosa_multiflora Rmu_sc0000288.1_g000035
rosa_roxburghii Rroxscaffold_6G00404500
rosa_rugosa Rorug03G0157400
rosa_samantha Rh3AG207500 Rh3BG240100 Rh3CG233800 Rh3CG234900 Rh3DG233800
rosa_wichuraiana Rw0G011130 Rw3G018890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 885
AccB1I GGYRCC 2 cut(s) 911, 2002
AccB7I CCANNNNNTGG 1 cut(s) 503
AccI GTMKAC 2 cut(s) 528, 1876
AccII CGCG 1 cut(s) 859
AciI CCGC 4 cut(s) 569, 676, 883, 1350
AclI AACGTT 1 cut(s) 1096
AclWI GGATC 5 cut(s) 166, 382, 391, 395, 608
AcsI RAATTY 4 cut(s) 623, 1274, 1412, 1712
AcuI CTGAAG 1 cut(s) 1383
AdeI CACNNNGTG 1 cut(s) 592
AfaI GTAC 2 cut(s) 809, 1887
AfiI CCNNNNNNNGG 6 cut(s) 503, 844, 1594, 1595, 1840, 1907
AflII CTTAAG 1 cut(s) 1075
AjnI CCWGG 3 cut(s) 336, 502, 1173
AleI CACNNNNGTG 1 cut(s) 1700
AloI GAACNNNNNNTCC 2 cut(s) 281, 313
Alw21I GWGCWC 4 cut(s) 737, 816, 1290, 1980
Alw26I GTCTC 3 cut(s) 473, 1140, 1806
AlwI GGATC 5 cut(s) 166, 382, 391, 395, 608
AlwNI CAGNNNCTG 2 cut(s) 503, 1727
AoxI GGCC 5 cut(s) 256, 522, 578, 1584, 1707
ApeKI GCWGC 9 cut(s) 16, 72, 341, 344, 444, 1007, 1309, 1570, 1724
ApoI RAATTY 4 cut(s) 623, 1274, 1412, 1712
Asp700I GAANNNNTTC 1 cut(s) 1452
AspA2I CCTAGG 1 cut(s) 154
AspLEI GCGC 5 cut(s) 121, 753, 859, 1007, 1688
AspS9I GGNCC 5 cut(s) 257, 466, 506, 1062, 1585
AsuHPI GGTGA 4 cut(s) 16, 883, 1388, 1576
AsuII TTCGAA 1 cut(s) 696
AvaII GGWCC 3 cut(s) 466, 506, 1062
AvrII CCTAGG 1 cut(s) 154
BaeI ACNNNNGTAYC 2 cut(s) 1928, 1961
BamHI GGATCC 1 cut(s) 387
BanI GGYRCC 2 cut(s) 911, 2002
BanII GRGCYC 5 cut(s) 737, 816, 1290, 1387, 1980
BbsI GAAGAC 1 cut(s) 216
Bbv12I GWGCWC 4 cut(s) 737, 816, 1290, 1980
BbvCI CCTCAGC 1 cut(s) 1313
BbvI GCAGC 9 cut(s) 28, 59, 328, 331, 431, 1019, 1321, 1582, 1711
BccI CCATC 5 cut(s) 791, 914, 1073, 1576, 1874
BceAI ACGGC 1 cut(s) 509
BcgI CGANNNNNNTGC 4 cut(s) 1665, 1699, 1970, 2004
BciT130I CCWGG 3 cut(s) 338, 504, 1175
BclI TGATCA 1 cut(s) 1669
BcoDI GTCTC 3 cut(s) 473, 1140, 1806
BfaI CTAG 6 cut(s) 155, 198, 401, 1235, 1775, 1787
BfrI CTTAAG 1 cut(s) 1075
BfuAI ACCTGC 1 cut(s) 885
BglI GCCNNNNNGGC 3 cut(s) 127, 577, 1358
BlnI CCTAGG 1 cut(s) 154
BlpI GCTNAGC 2 cut(s) 815, 1893
Bme1390I CCNGG 3 cut(s) 338, 504, 1175
Bme18I GGWCC 3 cut(s) 466, 506, 1062
BmgT120I GGNCC 5 cut(s) 257, 466, 506, 1062, 1585
BmiI GGNNCC 6 cut(s) 389, 725, 913, 1091, 1587, 2004
BmrFI CCNGG 3 cut(s) 338, 504, 1175
BmrI ACTGGG 2 cut(s) 1144, 1390
BmsI GCATC 5 cut(s) 256, 555, 1495, 1557, 1753
BmuI ACTGGG 2 cut(s) 1144, 1390
BoxI GACNNNNGTC 1 cut(s) 368
BpiI GAAGAC 1 cut(s) 216
BplI GAGNNNNNCTC 2 cut(s) 1349, 1381
BpmI CTGGAG 2 cut(s) 305, 1108
Bpu10I CCTNAGC 2 cut(s) 305, 1313
Bpu1102I GCTNAGC 2 cut(s) 815, 1893
Bpu14I TTCGAA 1 cut(s) 696
BpuEI CTTGAG 3 cut(s) 103, 630, 1274
Bsa29I ATCGAT 1 cut(s) 161
BsaAI YACGTR 1 cut(s) 1408
BsaI GGTCTC 2 cut(s) 1140, 1806
BsaJI CCNNGG 4 cut(s) 154, 843, 1353, 1817
BsaXI ACNNNNNCTCC 4 cut(s) 468, 498, 1081, 1111
Bsc4I CCNNNNNNNGG 6 cut(s) 503, 844, 1594, 1595, 1840, 1907
Bse1I ACTGG 5 cut(s) 288, 1064, 1150, 1385, 1702
Bse3DI GCAATG 3 cut(s) 581, 1068, 1467
BseBI CCWGG 3 cut(s) 338, 504, 1175
BseCI ATCGAT 1 cut(s) 161
BseDI CCNNGG 4 cut(s) 154, 843, 1353, 1817
BseGI GGATG 4 cut(s) 58, 324, 1183, 1288
BseLI CCNNNNNNNGG 6 cut(s) 503, 844, 1594, 1595, 1840, 1907
BseMI GCAATG 3 cut(s) 581, 1068, 1467
BseMII CTCAG 8 cut(s) 56, 174, 319, 722, 829, 1260, 1304, 1907
BseNI ACTGG 5 cut(s) 288, 1064, 1150, 1385, 1702
BseXI GCAGC 9 cut(s) 28, 59, 328, 331, 431, 1019, 1321, 1582, 1711
BsgI GTGCAG 1 cut(s) 1650
Bsh1236I CGCG 1 cut(s) 859
Bsh1285I CGRYCG 1 cut(s) 1142
BshFI GGCC 5 cut(s) 258, 524, 580, 1586, 1709
BshNI GGYRCC 2 cut(s) 911, 2002
BshVI ATCGAT 1 cut(s) 161
BsiEI CGRYCG 1 cut(s) 1142
BsiHKAI GWGCWC 4 cut(s) 737, 816, 1290, 1980
BslFI GGGAC 2 cut(s) 1132, 1821
BslI CCNNNNNNNGG 6 cut(s) 503, 844, 1594, 1595, 1840, 1907
BsmAI GTCTC 3 cut(s) 473, 1140, 1806
BsmFI GGGAC 2 cut(s) 1132, 1821
BsmI GAATGC 1 cut(s) 75
BsnI GGCC 5 cut(s) 258, 524, 580, 1586, 1709
Bso31I GGTCTC 2 cut(s) 1140, 1806
Bsp119I TTCGAA 1 cut(s) 696
Bsp1286I GDGCHC 5 cut(s) 737, 816, 1290, 1387, 1980
Bsp1407I TGTACA 1 cut(s) 1885
Bsp1720I GCTNAGC 2 cut(s) 815, 1893
Bsp19I CCATGG 1 cut(s) 1353
BspACI CCGC 4 cut(s) 569, 676, 883, 1350
BspANI GGCC 5 cut(s) 258, 524, 580, 1586, 1709
BspCNI CTCAG 8 cut(s) 57, 175, 318, 723, 828, 1261, 1305, 1906
BspDI ATCGAT 1 cut(s) 161
BspFNI CGCG 1 cut(s) 859
BspLI GGNNCC 6 cut(s) 389, 725, 913, 1091, 1587, 2004
BspMI ACCTGC 1 cut(s) 885
BspPI GGATC 5 cut(s) 166, 382, 391, 395, 608
BspT104I TTCGAA 1 cut(s) 696
BspT107I GGYRCC 2 cut(s) 911, 2002
BspTI CTTAAG 1 cut(s) 1075
BspTNI GGTCTC 2 cut(s) 1140, 1806
BsrDI GCAATG 3 cut(s) 581, 1068, 1467
BsrGI TGTACA 1 cut(s) 1885
BsrI ACTGG 5 cut(s) 288, 1064, 1150, 1385, 1702
BssECI CCNNGG 4 cut(s) 154, 843, 1353, 1817
BssT1I CCWWGG 3 cut(s) 154, 843, 1353
Bst2UI CCWGG 3 cut(s) 338, 504, 1175
Bst4CI ACNGT 4 cut(s) 421, 484, 592, 631
Bst6I CTCTTC 1 cut(s) 513
BstAFI CTTAAG 1 cut(s) 1075
BstAPI GCANNNNNTGC 1 cut(s) 673
BstAUI TGTACA 1 cut(s) 1885
BstBAI YACGTR 1 cut(s) 1408
BstBI TTCGAA 1 cut(s) 696
BstC8I GCNNGC 4 cut(s) 460, 1363, 1684, 1797
BstDEI CTNAG 8 cut(s) 65, 183, 305, 731, 815, 1269, 1313, 1893
BstDSI CCRYGG 2 cut(s) 1353, 1817
BstF5I GGATG 4 cut(s) 58, 324, 1183, 1288
BstFNI CGCG 1 cut(s) 859
BstHHI GCGC 5 cut(s) 121, 753, 859, 1007, 1688
BstMAI GTCTC 3 cut(s) 473, 1140, 1806
BstMCI CGRYCG 1 cut(s) 1142
BstMWI GCNNNNNNNGC 8 cut(s) 118, 127, 227, 577, 673, 854, 1358, 1792
BstNI CCWGG 3 cut(s) 338, 504, 1175
BstNSI RCATGY 5 cut(s) 444, 667, 1365, 1770, 1892
BstPAI GACNNNNGTC 1 cut(s) 368
BstSCI CCNGG 3 cut(s) 336, 502, 1173
BstUI CGCG 1 cut(s) 859
BstV1I GCAGC 9 cut(s) 28, 59, 328, 331, 431, 1019, 1321, 1582, 1711
BstV2I GAAGAC 1 cut(s) 216
BstX2I RGATCY 1 cut(s) 387
BstYI RGATCY 1 cut(s) 387
Bsu15I ATCGAT 1 cut(s) 161
BsuRI GGCC 5 cut(s) 258, 524, 580, 1586, 1709
BsuTUI ATCGAT 1 cut(s) 161
BtgI CCRYGG 2 cut(s) 1353, 1817
BtgZI GCGATG 1 cut(s) 151
BtsCI GGATG 4 cut(s) 58, 324, 1183, 1288
BtsIMutI CAGTG 4 cut(s) 281, 588, 793, 1695
BveI ACCTGC 1 cut(s) 885
Cac8I GCNNGC 4 cut(s) 460, 1363, 1684, 1797
CaiI CAGNNNCTG 2 cut(s) 503, 1727
CfoI GCGC 5 cut(s) 121, 753, 859, 1007, 1688
Cfr13I GGNCC 5 cut(s) 257, 466, 506, 1062, 1585
ClaI ATCGAT 1 cut(s) 161
CseI GACGC 1 cut(s) 1172
CsiI ACCWGGT 1 cut(s) 502
Csp6I GTAC 2 cut(s) 808, 1886
CviQI GTAC 2 cut(s) 808, 1886
DdeI CTNAG 8 cut(s) 65, 183, 305, 731, 815, 1269, 1313, 1893
DraI TTTAAA 1 cut(s) 657
DraIII CACNNNGTG 1 cut(s) 592
Eam1104I CTCTTC 1 cut(s) 513
EarI CTCTTC 1 cut(s) 513
Ecl136II GAGCTC 4 cut(s) 735, 814, 1288, 1978
Eco130I CCWWGG 3 cut(s) 154, 843, 1353
Eco24I GRGCYC 5 cut(s) 737, 816, 1290, 1387, 1980
Eco31I GGTCTC 2 cut(s) 1140, 1806
Eco47I GGWCC 3 cut(s) 466, 506, 1062
Eco53kI GAGCTC 4 cut(s) 735, 814, 1288, 1978
Eco57I CTGAAG 1 cut(s) 1383
EcoICRI GAGCTC 4 cut(s) 735, 814, 1288, 1978
EcoRII CCWGG 3 cut(s) 336, 502, 1173
EcoT14I CCWWGG 3 cut(s) 154, 843, 1353
EcoT22I ATGCAT 1 cut(s) 1768
EcoT38I GRGCYC 5 cut(s) 737, 816, 1290, 1387, 1980
ErhI CCWWGG 3 cut(s) 154, 843, 1353
FalI AAGNNNNNCTT 2 cut(s) 1631, 1663
FaqI GGGAC 2 cut(s) 1132, 1821
FauI CCCGC 1 cut(s) 669
FbaI TGATCA 1 cut(s) 1669
FblI GTMKAC 2 cut(s) 528, 1876
FokI GGATG 4 cut(s) 65, 311, 1190, 1295
FriOI GRGCYC 5 cut(s) 737, 816, 1290, 1387, 1980
FspBI CTAG 6 cut(s) 155, 198, 401, 1235, 1775, 1787
GlaI GCGC 5 cut(s) 120, 752, 858, 1006, 1687
GsuI CTGGAG 2 cut(s) 305, 1108
HaeIII GGCC 5 cut(s) 258, 524, 580, 1586, 1709
HgaI GACGC 1 cut(s) 1172
HhaI GCGC 5 cut(s) 121, 753, 859, 1007, 1688
Hin6I GCGC 5 cut(s) 119, 751, 857, 1005, 1686
HinP1I GCGC 5 cut(s) 119, 751, 857, 1005, 1686
HincII GTYRAC 2 cut(s) 529, 634
HindII GTYRAC 2 cut(s) 529, 634
HindIII AAGCTT 2 cut(s) 460, 700
HinfI GANTC 8 cut(s) 61, 316, 650, 680, 711, 1230, 1644, 1836
HphI GGTGA 4 cut(s) 16, 883, 1388, 1576
Hpy166II GTNNAC 5 cut(s) 529, 634, 891, 1200, 1877
Hpy188I TCNGA 9 cut(s) 66, 732, 1057, 1270, 1402, 1543, 1669, 1718, 1923
Hpy8I GTNNAC 5 cut(s) 529, 634, 891, 1200, 1877
Hpy99I CGWCG 2 cut(s) 397, 530
HpyAV CCTTC 6 cut(s) 737, 904, 1116, 1122, 1699, 1726
HpyCH4III ACNGT 4 cut(s) 421, 484, 592, 631
HpyCH4IV ACGT 2 cut(s) 1096, 1407
HpyF10VI GCNNNNNNNGC 8 cut(s) 118, 127, 227, 577, 673, 854, 1358, 1792
HpyF3I CTNAG 8 cut(s) 65, 183, 305, 731, 815, 1269, 1313, 1893
HpySE526I ACGT 2 cut(s) 1096, 1407
HspAI GCGC 5 cut(s) 119, 751, 857, 1005, 1686
Ksp22I TGATCA 1 cut(s) 1669
LmnI GCTCC 4 cut(s) 156, 740, 885, 1089
Lsp1109I GCAGC 9 cut(s) 28, 59, 328, 331, 431, 1019, 1321, 1582, 1711
LweI GCATC 5 cut(s) 256, 555, 1495, 1557, 1753
MabI ACCWGGT 1 cut(s) 502
MaeI CTAG 6 cut(s) 155, 198, 401, 1235, 1775, 1787
MaeII ACGT 2 cut(s) 1096, 1407
MaeIII GTNAC 2 cut(s) 640, 1376
MboII GAAGA 7 cut(s) 221, 530, 685, 1254, 1448, 1514, 1550
MfeI CAATTG 1 cut(s) 1881
MflI RGATCY 1 cut(s) 387
MhlI GDGCHC 5 cut(s) 737, 816, 1290, 1387, 1980
MluCI AATT 9 cut(s) 623, 1168, 1274, 1412, 1600, 1712, 1847, 1881, 1967
MlyI GAGTC 2 cut(s) 705, 1845
Mph1103I ATGCAT 1 cut(s) 1768
MroXI GAANNNNTTC 1 cut(s) 1452
MseI TTAA 3 cut(s) 656, 1076, 1319
MslI CAYNNNNRTG 2 cut(s) 252, 1700
MspA1I CMGCKG 2 cut(s) 1312, 1724
MspCI CTTAAG 1 cut(s) 1075
MspR9I CCNGG 3 cut(s) 338, 504, 1175
MunI CAATTG 1 cut(s) 1881
Mva1269I GAATGC 1 cut(s) 75
MvaI CCWGG 3 cut(s) 338, 504, 1175
MvnI CGCG 1 cut(s) 859
MwoI GCNNNNNNNGC 8 cut(s) 118, 127, 227, 577, 673, 854, 1358, 1792
NcoI CCATGG 1 cut(s) 1353
NlaIV GGNNCC 6 cut(s) 389, 725, 913, 1091, 1587, 2004
NmeAIII GCCGAG 3 cut(s) 1714, 1859, 1932
NmuCI GTSAC 1 cut(s) 1376
NsiI ATGCAT 1 cut(s) 1768
NspI RCATGY 5 cut(s) 444, 667, 1365, 1770, 1892
NspV TTCGAA 1 cut(s) 696
OliI CACNNNNGTG 1 cut(s) 1700
PaeI GCATGC 1 cut(s) 1365
PctI GAATGC 1 cut(s) 75
PdmI GAANNNNTTC 1 cut(s) 1452
PfeI GAWTC 6 cut(s) 61, 316, 650, 680, 1230, 1644
PflMI CCANNNNNTGG 1 cut(s) 503
Ple19I CGATCG 1 cut(s) 1142
PleI GAGTC 2 cut(s) 705, 1844
PpsI GAGTC 2 cut(s) 705, 1844
Ppu21I YACGTR 1 cut(s) 1408
PshAI GACNNNNGTC 1 cut(s) 368
Psp124BI GAGCTC 4 cut(s) 737, 816, 1290, 1980
Psp1406I AACGTT 1 cut(s) 1096
Psp6I CCWGG 3 cut(s) 336, 502, 1173
PspGI CCWGG 3 cut(s) 336, 502, 1173
PspN4I GGNNCC 6 cut(s) 389, 725, 913, 1091, 1587, 2004
PspPI GGNCC 5 cut(s) 257, 466, 506, 1062, 1585
PstNI CAGNNNCTG 2 cut(s) 503, 1727
PsuI RGATCY 1 cut(s) 387
PvuI CGATCG 1 cut(s) 1142
PvuII CAGCTG 2 cut(s) 1312, 1724
RsaI GTAC 2 cut(s) 809, 1887
RsaNI GTAC 2 cut(s) 808, 1886
RseI CAYNNNNRTG 2 cut(s) 252, 1700
SacI GAGCTC 4 cut(s) 737, 816, 1290, 1980
SalI GTCGAC 1 cut(s) 527
SaqAI TTAA 3 cut(s) 656, 1076, 1319
Sau96I GGNCC 5 cut(s) 257, 466, 506, 1062, 1585
SchI GAGTC 2 cut(s) 705, 1845
ScrFI CCNGG 3 cut(s) 338, 504, 1175
SduI GDGCHC 5 cut(s) 737, 816, 1290, 1387, 1980
SexAI ACCWGGT 1 cut(s) 502
SfaNI GCATC 5 cut(s) 256, 555, 1495, 1557, 1753
SfuI TTCGAA 1 cut(s) 696
SinI GGWCC 3 cut(s) 466, 506, 1062
SmiMI CAYNNNNRTG 2 cut(s) 252, 1700
SmlI CTYRAG 4 cut(s) 82, 645, 1075, 1289
SmoI CTYRAG 4 cut(s) 82, 645, 1075, 1289
SphI GCATGC 1 cut(s) 1365
Sse9I AATT 9 cut(s) 623, 1168, 1274, 1412, 1600, 1712, 1847, 1881, 1967
SsiI CCGC 4 cut(s) 569, 676, 883, 1350
SspMI CTAG 6 cut(s) 155, 198, 401, 1235, 1775, 1787
SstI GAGCTC 4 cut(s) 737, 816, 1290, 1980
StyD4I CCNGG 3 cut(s) 336, 502, 1173
StyI CCWWGG 3 cut(s) 154, 843, 1353
TaaI ACNGT 4 cut(s) 421, 484, 592, 631
TaiI ACGT 2 cut(s) 1099, 1410
TasI AATT 9 cut(s) 623, 1168, 1274, 1412, 1600, 1712, 1847, 1881, 1967
TatI WGTACW 1 cut(s) 1885
TauI GCSGC 1 cut(s) 571
TfiI GAWTC 6 cut(s) 61, 316, 650, 680, 1230, 1644
Tru1I TTAA 3 cut(s) 656, 1076, 1319
Tru9I TTAA 3 cut(s) 656, 1076, 1319
TscAI CASTG 4 cut(s) 288, 595, 793, 1702
TseFI GTSAC 1 cut(s) 1376
TseI GCWGC 9 cut(s) 16, 72, 341, 344, 444, 1007, 1309, 1570, 1724
Tsp45I GTSAC 1 cut(s) 1376
TspDTI ATGAA 7 cut(s) 56, 222, 531, 811, 916, 1098, 1288
TspGWI ACGGA 2 cut(s) 651, 1295
TspRI CASTG 4 cut(s) 288, 595, 793, 1702
Van91I CCANNNNNTGG 1 cut(s) 503
Vha464I CTTAAG 1 cut(s) 1075
VpaK11BI GGWCC 3 cut(s) 466, 506, 1062
XapI RAATTY 4 cut(s) 623, 1274, 1412, 1712
XbaI TCTAGA 1 cut(s) 197
XceI RCATGY 5 cut(s) 444, 667, 1365, 1770, 1892
XcmI CCANNNNNNNNNTGG 1 cut(s) 850
XmaJI CCTAGG 1 cut(s) 154
XmiI GTMKAC 2 cut(s) 528, 1876
XmnI GAANNNNTTC 1 cut(s) 1452
XspI CTAG 6 cut(s) 155, 198, 401, 1235, 1775, 1787
Zsp2I ATGCAT 1 cut(s) 1768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.