Rh3CG234900

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0016402
Physical Location & Seq
Reverse (-)
22251668 .. 22253806
2139 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG234900.1

Sequence Viewer

Length: 2139 bp
ATGGATAAAATTAGGGAGATACCATTTTTCTCCAAAGAGGTGAAGAAGATATGGGACGTGTGGAATCTGCAGGGCTGTATTCTCTTAAGTCTCTCACTGCAAGTTTCTCTGCTCTTGGCATCATTTCGGAAAAAATGCAGAAGCAAATTGCTACGAGGACTCATCTGGTCTGCGTATTTGCTCGCCGATTGGATTGCTGCTGTTGCAATAGGGATAATCACCAAATCCCAAGTTGACACTTGCGACCCGAAGGGCAACAAAGATCTTTTGGCATTCTGGGCTTCCTTTCTTCTACTCCATCTTGGCGGCCCTGATACCATTACCTCTTTTGCTCTTGAGGACAACGAGTTCTGGGTTAGGCACTTGGTTGGACTAATTGTTCAAGTTCTTGCAGCTGCTTGTTGTTTCTACCTGACACTTCATAACAAGTTCTTGGTTCCGACAGTACTAGTCTTTGTTGTTGGAACAATCAAGTTTGGGGAGAGAACTTGTGCTCTGTATCTTGCAAGTCTAGACCATTTTGGGGAGACTGTATTGCCAGAGCCGGTGGCTGGACCTGATTATGAAGAAACTGTAGCAATCCATACTTCAATGAGGTCAGTCGCAGTCCCTACGACAAAAGAAGTAGTGGTACCAATCAGGCCATATATTGGGGATGCACATAAAGATCCAAAGTTTACGATTAAGGGAAATACAAAAGAATATTTAAACGACATGCAATTGCTGCAAGAAGCGTATTACCTCTTTGAGAGCTTCAAAAGACTCATTGCTGGCTTTCTTCTCAGCTCCAAAGACCGAGAACTGAGTCGAAATTTCTTCCTTGAAAGAACCTATACTAGCGCTTTTCAGTTGATAGAGTACGAGCTCAGCTTCATGTATGAGGTTCTTCACACCAAGGTTATTGTGGTGCGTCGCAAAATTGGGTACACCCTCCGCTTAGTAAGCTTCTGTGCAACCATTGTTGCCTTGGTGTTCTTCTTTTCGGTTGAAAAGAAAAAGTTTGTCAAGTTTGACATTGATCTTACTTATGCTTTGTTTAGTGGAGCCATTTTCCTGGACATCATATCTGTCTTTATGCTTATCTTCTCTGACTGGACCCTCATTGCCTTCAACAACACTAACATTTGGAGCAGATTTATTGCTGCTGTAATAATGAAAAGAGGAAGGTGGTCTGGATCGGTATCCCAATACAATATGATAAAGAATTGCCTGGATGAGCGTCCAGAATGTGTCTTGACTCTTGCCGGTTATCTGGGTGTTGGGGGAATTCTAGGCAGAACCAAAATTGTCTACTTTTCATCCTCAGAAGCAGTCACCGAAGAGCTGAAGTACTTTATCTTCAGGCAGCTGAGGTTAAAATCTCTGAAAGCAAACAACAGAATGGATGGAATGGAGGGATGTTCACAAAGAGGTGACTGGGCTCTGCTGCAGAATTCAAAGTATCAGAAACTCAGATGGAGCATTGGGGATTATCAATATGCGGAAAGTGTTCTTCTCTGGCACATTGCAACAGAACTCTACTATGCTGAAAGTGGAACCGAGCAATGTACTGATTGTGGTCAAGGTAGACAAGCAACACAAAGGGAATGTGATAGTGACAATCGACTAAAACTTTGCAATTTTTGTGAAAGTAACGCCGCGCGTAGTATTTGCAAACTTGTTTCAGATTATCTGTTTTATCTTCTGGTGATGCAGCATCCAATGATGGCCCCAGTTTTGGGGAATTGGCATATAGTGTTCCAGGACACTTGTGCAGAGGCCAAGAGATTCTTTCGCAAGCATTCAGTAAAAGATCATAAGGAAGCCTGTAAAAAGATTTCTGATGTCAAAGCCCAATTCAGATCGGCTGCTGTGAAGGGAAGTAAAAGCAAATCTGTGCTGTTCGATGCATGTATACTTGCAGAACAGCTTAAGGAGTTGGAGGCAGAGCAGGACCCATGGGAGCTAATGAGCCGTGTGTGGGTGGAATTATTGTCATATGCTTCCATTAAATGTAGACCGATTGTACATGCTCAGCAACCAAGTAGGGGTGGAGAACTGATGACTTTCACTTGGCTACTGATGAATCATCTGGGCTTGGGAACACAATTCTACGAGCTGGAAAACCAAGCTGGAACAAAAATGGTGGCAGACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

712

Amino Acids

81.35

Weight (kDa)

8.63

Isoelectric Point (pI)

36.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 56 - 401 4.5e-92 Domain of unknown function (DUF4220)
DUF594 PF04578 641 - 693 4.5e-18 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016402)

Species Orthologous Gene IDs
malus_domestica MD10G1305500.v1.1
prunus_persica Prupe.4G034200_v2.0.a1
pyrus_communis pycom10g25790
rosa_chinensis RchiOBHm_Chr3g0476871
rosa_laevigata RLG00000023747
rosa_multiflora Rmu_sc0000288.1_g000035
rosa_roxburghii Rroxscaffold_6G00404500
rosa_rugosa Rorug03G0157400
rosa_samantha Rh3AG207500 Rh3BG240100 Rh3CG233800 Rh3CG234900 Rh3DG233800
rosa_wichuraiana Rw0G011130 Rw3G018890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 631
AccB1I GGYRCC 1 cut(s) 631
AccB7I CCANNNNNTGG 1 cut(s) 650
AccI GTMKAC 4 cut(s) 1290, 1567, 1894, 1996
AccII CGCG 2 cut(s) 1640, 1642
AciI CCGC 4 cut(s) 306, 934, 1481, 1638
AclWI GGATC 2 cut(s) 662, 1183
AcsI RAATTY 3 cut(s) 811, 1266, 1432
AcuI CTGAAG 2 cut(s) 1324, 1346
AfaI GTAC 7 cut(s) 447, 633, 860, 926, 1331, 1549, 2007
AfeI AGCGCT 1 cut(s) 841
AfiI CCNNNNNNNGG 7 cut(s) 523, 551, 650, 1717, 1718, 1960, 2027
AflII CTTAAG 2 cut(s) 85, 1910
AflIII ACRYGT 1 cut(s) 57
AgsI TTSAA 7 cut(s) 383, 591, 757, 824, 989, 1111, 1437
AhlI ACTAGT 1 cut(s) 448
AjiI CACGTC 1 cut(s) 58
AjnI CCWGG 3 cut(s) 1053, 1209, 1740
AloI GAACNNNNNNTCC 4 cut(s) 332, 363, 364, 395
Alw21I GWGCWC 2 cut(s) 496, 867
Alw26I GTCTC 2 cut(s) 95, 521
AlwI GGATC 2 cut(s) 662, 1183
Aor51HI AGCGCT 1 cut(s) 841
AoxI GGCC 4 cut(s) 307, 641, 1707, 1758
ApeKI GCWGC 9 cut(s) 197, 392, 395, 724, 1142, 1345, 1426, 1693, 1847
ApoI RAATTY 3 cut(s) 811, 1266, 1432
ArsI GACNNNNNNTTYG 2 cut(s) 1596, 1628
Asp700I GAANNNNTTC 1 cut(s) 1488
Asp718I GGTACC 1 cut(s) 631
AspLEI GCGC 2 cut(s) 842, 1642
AspS9I GGNCC 5 cut(s) 308, 554, 1095, 1708, 1933
AsuHPI GGTGA 5 cut(s) 52, 211, 1306, 1424, 1699
AvaII GGWCC 3 cut(s) 554, 1095, 1933
BanI GGYRCC 1 cut(s) 631
BanII GRGCYC 2 cut(s) 867, 1423
BarI GAAGNNNNNNTAC 4 cut(s) 615, 647, 1322, 1354
Bbv12I GWGCWC 2 cut(s) 496, 867
BbvCI CCTCAGC 1 cut(s) 1349
BbvI GCAGC 9 cut(s) 184, 382, 404, 711, 1129, 1357, 1413, 1705, 1834
BccI CCATC 4 cut(s) 306, 1379, 1449, 1699
BceAI ACGGC 1 cut(s) 1938
BcgI CGANNNNNNTGC 2 cut(s) 176, 210
BciT130I CCWGG 3 cut(s) 1055, 1211, 1742
BciVI GTATCC 1 cut(s) 1192
BcoDI GTCTC 2 cut(s) 95, 521
BcuI ACTAGT 1 cut(s) 448
BfaI CTAG 4 cut(s) 449, 512, 837, 1271
BfmI CTRYAG 3 cut(s) 68, 573, 1427
BfoI RGCGCY 1 cut(s) 843
BfrI CTTAAG 2 cut(s) 85, 1910
BfuI GTATCC 1 cut(s) 1192
BglII AGATCT 1 cut(s) 262
BlpI GCTNAGC 2 cut(s) 866, 2013
BmcAI AGTACT 2 cut(s) 447, 1331
Bme1390I CCNGG 3 cut(s) 1055, 1211, 1742
Bme18I GGWCC 3 cut(s) 554, 1095, 1933
BmgBI CACGTC 1 cut(s) 58
BmgT120I GGNCC 5 cut(s) 308, 554, 1095, 1708, 1933
BmiI GGNNCC 7 cut(s) 438, 633, 1045, 1097, 1537, 1710, 1935
BmrFI CCNGG 3 cut(s) 1055, 1211, 1742
BmrI ACTGGG 2 cut(s) 1426, 1706
BmsI GCATC 5 cut(s) 128, 646, 1680, 1705, 1876
BmuI ACTGGG 2 cut(s) 1426, 1706
Bpu10I CCTNAGC 1 cut(s) 1349
Bpu1102I GCTNAGC 2 cut(s) 866, 2013
BpuEI CTTGAG 1 cut(s) 356
BsaBI GATNNNNATC 1 cut(s) 1180
BsaJI CCNNGG 3 cut(s) 894, 966, 1937
Bsc4I CCNNNNNNNGG 7 cut(s) 523, 551, 650, 1717, 1718, 1960, 2027
Bse118I RCCGGY 2 cut(s) 544, 1244
Bse1I ACTGG 3 cut(s) 1097, 1421, 1712
Bse3DI GCAATG 4 cut(s) 765, 1101, 1503, 1550
Bse8I GATNNNNATC 1 cut(s) 1180
BseBI CCWGG 3 cut(s) 1055, 1211, 1742
BseDI CCNNGG 3 cut(s) 894, 966, 1937
BseGI GGATG 6 cut(s) 661, 1219, 1298, 1390, 1403, 1696
BseJI GATNNNNATC 1 cut(s) 1180
BseLI CCNNNNNNNGG 7 cut(s) 523, 551, 650, 1717, 1718, 1960, 2027
BseMI GCAATG 4 cut(s) 765, 1101, 1503, 1550
BseMII CTCAG 7 cut(s) 794, 796, 880, 1317, 1340, 1465, 2027
BseNI ACTGG 3 cut(s) 1097, 1421, 1712
BseXI GCAGC 9 cut(s) 184, 382, 404, 711, 1129, 1357, 1413, 1705, 1834
BsgI GTGCAG 1 cut(s) 1773
Bsh1236I CGCG 2 cut(s) 1640, 1642
BshFI GGCC 4 cut(s) 309, 643, 1709, 1760
BshNI GGYRCC 1 cut(s) 631
BsiHKAI GWGCWC 2 cut(s) 496, 867
BsiSI CCGG 2 cut(s) 545, 1245
BslFI GGGAC 2 cut(s) 68, 593
BslI CCNNNNNNNGG 7 cut(s) 523, 551, 650, 1717, 1718, 1960, 2027
BsmAI GTCTC 2 cut(s) 95, 521
BsmFI GGGAC 2 cut(s) 68, 593
BsmI GAATGC 2 cut(s) 272, 1780
BsnI GGCC 4 cut(s) 309, 643, 1709, 1760
Bsp1286I GDGCHC 3 cut(s) 496, 867, 1423
Bsp1407I TGTACA 1 cut(s) 2005
Bsp143I GATC 6 cut(s) 262, 667, 1018, 1175, 1792, 1841
Bsp1720I GCTNAGC 2 cut(s) 866, 2013
Bsp19I CCATGG 1 cut(s) 1937
BspACI CCGC 4 cut(s) 306, 934, 1481, 1638
BspANI GGCC 4 cut(s) 309, 643, 1709, 1760
BspCNI CTCAG 7 cut(s) 795, 795, 879, 1316, 1341, 1464, 2026
BspFNI CGCG 2 cut(s) 1640, 1642
BspLI GGNNCC 7 cut(s) 438, 633, 1045, 1097, 1537, 1710, 1935
BspMAI CTGCAG 2 cut(s) 72, 1431
BspPI GGATC 2 cut(s) 662, 1183
BspQI GCTCTTC 1 cut(s) 1314
BspT107I GGYRCC 1 cut(s) 631
BspTI CTTAAG 2 cut(s) 85, 1910
BsrDI GCAATG 4 cut(s) 765, 1101, 1503, 1550
BsrFI RCCGGY 2 cut(s) 544, 1244
BsrGI TGTACA 1 cut(s) 2005
BsrI ACTGG 3 cut(s) 1097, 1421, 1712
BssAI RCCGGY 2 cut(s) 544, 1244
BssECI CCNNGG 3 cut(s) 894, 966, 1937
BssMI GATC 6 cut(s) 262, 667, 1018, 1175, 1792, 1841
BssNAI GTATAC 1 cut(s) 1895
BssT1I CCWWGG 3 cut(s) 894, 966, 1937
Bst1107I GTATAC 1 cut(s) 1895
Bst2UI CCWGG 3 cut(s) 1055, 1211, 1742
Bst4CI ACNGT 3 cut(s) 445, 532, 574
Bst6I CTCTTC 1 cut(s) 1314
BstAFI CTTAAG 2 cut(s) 85, 1910
BstAPI GCANNNNNTGC 1 cut(s) 724
BstAUI TGTACA 1 cut(s) 2005
BstC8I GCNNGC 3 cut(s) 183, 772, 1778
BstDEI CTNAG 8 cut(s) 782, 803, 866, 937, 1303, 1349, 1451, 2013
BstDSI CCRYGG 1 cut(s) 1937
BstF5I GGATG 6 cut(s) 661, 1219, 1298, 1390, 1403, 1696
BstFNI CGCG 2 cut(s) 1640, 1642
BstH2I RGCGCY 1 cut(s) 843
BstHHI GCGC 2 cut(s) 842, 1642
BstKTI GATC 6 cut(s) 265, 670, 1021, 1178, 1795, 1844
BstMAI GTCTC 2 cut(s) 95, 521
BstMBI GATC 6 cut(s) 262, 667, 1018, 1175, 1792, 1841
BstMWI GCNNNNNNNGC 4 cut(s) 203, 278, 724, 942
BstNI CCWGG 3 cut(s) 1055, 1211, 1742
BstNSI RCATGY 3 cut(s) 718, 1893, 2012
BstSCI CCNGG 3 cut(s) 1053, 1209, 1740
BstSFI CTRYAG 3 cut(s) 68, 573, 1427
BstUI CGCG 2 cut(s) 1640, 1642
BstV1I GCAGC 9 cut(s) 184, 382, 404, 711, 1129, 1357, 1413, 1705, 1834
BstX2I RGATCY 2 cut(s) 262, 667
BstXI CCANNNNNNTGG 1 cut(s) 1054
BstYI RGATCY 2 cut(s) 262, 667
BstZ17I GTATAC 1 cut(s) 1895
BsuI GTATCC 1 cut(s) 1192
BsuRI GGCC 4 cut(s) 309, 643, 1709, 1760
BtgI CCRYGG 1 cut(s) 1937
BtrI CACGTC 1 cut(s) 58
BtsCI GGATG 6 cut(s) 661, 1219, 1298, 1390, 1403, 1696
BtsI GCAGTG 1 cut(s) 95
BtsIMutI CAGTG 1 cut(s) 95
Cac8I GCNNGC 3 cut(s) 183, 772, 1778
CfoI GCGC 2 cut(s) 842, 1642
Cfr10I RCCGGY 2 cut(s) 544, 1244
Cfr13I GGNCC 5 cut(s) 308, 554, 1095, 1708, 1933
CseI GACGC 2 cut(s) 899, 1208
Csp6I GTAC 7 cut(s) 446, 632, 859, 925, 1330, 1548, 2006
CspCI CAANNNNNGTGG 1 cut(s) 2106
CviAII CATG 5 cut(s) 715, 874, 1890, 1938, 2009
CviQI GTAC 7 cut(s) 446, 632, 859, 925, 1330, 1548, 2006
DdeI CTNAG 8 cut(s) 782, 803, 866, 937, 1303, 1349, 1451, 2013
DpnI GATC 6 cut(s) 264, 669, 1020, 1177, 1794, 1843
DpnII GATC 6 cut(s) 262, 667, 1018, 1175, 1792, 1841
DraI TTTAAA 1 cut(s) 708
Eam1104I CTCTTC 1 cut(s) 1314
EarI CTCTTC 1 cut(s) 1314
Ecl136II GAGCTC 1 cut(s) 865
Eco130I CCWWGG 3 cut(s) 894, 966, 1937
Eco24I GRGCYC 2 cut(s) 867, 1423
Eco47I GGWCC 3 cut(s) 554, 1095, 1933
Eco47III AGCGCT 1 cut(s) 841
Eco53kI GAGCTC 1 cut(s) 865
Eco57I CTGAAG 2 cut(s) 1324, 1346
EcoICRI GAGCTC 1 cut(s) 865
EcoO109I RGGNCCY 1 cut(s) 1933
EcoRI GAATTC 2 cut(s) 1266, 1432
EcoRII CCWGG 3 cut(s) 1053, 1209, 1740
EcoT14I CCWWGG 3 cut(s) 894, 966, 1937
EcoT22I ATGCAT 1 cut(s) 1891
EcoT38I GRGCYC 2 cut(s) 867, 1423
ErhI CCWWGG 3 cut(s) 894, 966, 1937
FaeI CATG 5 cut(s) 718, 877, 1893, 1941, 2012
FalI AAGNNNNNCTT 2 cut(s) 1754, 1786
FaqI GGGAC 2 cut(s) 68, 593
FatI CATG 5 cut(s) 714, 873, 1889, 1937, 2008
FauNDI CATATG 1 cut(s) 1978
FblI GTMKAC 4 cut(s) 1290, 1567, 1894, 1996
FokI GGATG 6 cut(s) 668, 1226, 1285, 1397, 1410, 1683
FriOI GRGCYC 2 cut(s) 867, 1423
FspBI CTAG 4 cut(s) 449, 512, 837, 1271
GlaI GCGC 2 cut(s) 841, 1641
HaeII RGCGCY 1 cut(s) 843
HaeIII GGCC 4 cut(s) 309, 643, 1709, 1760
HapII CCGG 2 cut(s) 545, 1245
HgaI GACGC 2 cut(s) 899, 1208
HhaI GCGC 2 cut(s) 842, 1642
Hin1II CATG 5 cut(s) 718, 877, 1893, 1941, 2012
Hin6I GCGC 2 cut(s) 840, 1640
HinP1I GCGC 2 cut(s) 840, 1640
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HindIII AAGCTT 1 cut(s) 943
HinfI GANTC 7 cut(s) 64, 159, 762, 805, 1237, 1767, 2065
HpaII CCGG 2 cut(s) 545, 1245
HphI GGTGA 5 cut(s) 52, 211, 1306, 1424, 1699
Hpy166II GTNNAC 8 cut(s) 235, 678, 927, 1291, 1403, 1568, 1895, 1997
Hpy188III TCNNGA 5 cut(s) 335, 512, 1173, 1223, 1234
Hpy8I GTNNAC 8 cut(s) 235, 678, 927, 1291, 1403, 1568, 1895, 1997
Hpy99I CGWCG 1 cut(s) 915
HpyAV CCTTC 4 cut(s) 244, 1117, 1158, 1849
HpyCH4III ACNGT 3 cut(s) 445, 532, 574
HpyCH4IV ACGT 1 cut(s) 57
HpyF10VI GCNNNNNNNGC 4 cut(s) 203, 278, 724, 942
HpyF3I CTNAG 8 cut(s) 782, 803, 866, 937, 1303, 1349, 1451, 2013
HpySE526I ACGT 1 cut(s) 57
Hsp92II CATG 5 cut(s) 718, 877, 1893, 1941, 2012
HspAI GCGC 2 cut(s) 840, 1640
KpnI GGTACC 1 cut(s) 635
Kzo9I GATC 6 cut(s) 262, 667, 1018, 1175, 1792, 1841
LguI GCTCTTC 1 cut(s) 1314
LmnI GCTCC 5 cut(s) 791, 1043, 1128, 1458, 1942
Lsp1109I GCAGC 9 cut(s) 184, 382, 404, 711, 1129, 1357, 1413, 1705, 1834
LweI GCATC 5 cut(s) 128, 646, 1680, 1705, 1876
MaeI CTAG 4 cut(s) 449, 512, 837, 1271
MaeII ACGT 1 cut(s) 57
MaeIII GTNAC 4 cut(s) 1312, 1412, 1595, 1631
MalI GATC 6 cut(s) 264, 669, 1020, 1177, 1794, 1843
MboI GATC 6 cut(s) 262, 667, 1018, 1175, 1792, 1841
MfeI CAATTG 1 cut(s) 719
MflI RGATCY 2 cut(s) 262, 667
MhlI GDGCHC 3 cut(s) 496, 867, 1423
MlyI GAGTC 4 cut(s) 153, 756, 814, 1231
MmeI TCCRAC 4 cut(s) 349, 442, 464, 1899
Mph1103I ATGCAT 1 cut(s) 1891
MroXI GAANNNNTTC 1 cut(s) 1488
MseI TTAA 6 cut(s) 86, 684, 707, 1355, 1911, 1989
MspA1I CMGCKG 2 cut(s) 395, 1348
MspCI CTTAAG 2 cut(s) 85, 1910
MspI CCGG 2 cut(s) 545, 1245
MspR9I CCNGG 3 cut(s) 1055, 1211, 1742
MunI CAATTG 1 cut(s) 719
Mva1269I GAATGC 2 cut(s) 272, 1780
MvaI CCWGG 3 cut(s) 1055, 1211, 1742
MvnI CGCG 2 cut(s) 1640, 1642
MwoI GCNNNNNNNGC 4 cut(s) 203, 278, 724, 942
NcoI CCATGG 1 cut(s) 1937
NdeI CATATG 1 cut(s) 1978
NdeII GATC 6 cut(s) 262, 667, 1018, 1175, 1792, 1841
NlaIII CATG 5 cut(s) 718, 877, 1893, 1941, 2012
NlaIV GGNNCC 7 cut(s) 438, 633, 1045, 1097, 1537, 1710, 1935
NmuCI GTSAC 3 cut(s) 1312, 1412, 1595
NsiI ATGCAT 1 cut(s) 1891
NspI RCATGY 3 cut(s) 718, 1893, 2012
PciSI GCTCTTC 1 cut(s) 1314
PctI GAATGC 2 cut(s) 272, 1780
PdmI GAANNNNTTC 1 cut(s) 1488
PfeI GAWTC 3 cut(s) 64, 1767, 2065
PflMI CCANNNNNTGG 1 cut(s) 650
PfoI TCCNGGA 2 cut(s) 1053, 1740
PleI GAGTC 4 cut(s) 153, 756, 813, 1231
PpsI GAGTC 4 cut(s) 153, 756, 813, 1231
PpuMI RGGWCCY 1 cut(s) 1933
Psp124BI GAGCTC 1 cut(s) 867
Psp5II RGGWCCY 1 cut(s) 1933
Psp6I CCWGG 3 cut(s) 1053, 1209, 1740
PspGI CCWGG 3 cut(s) 1053, 1209, 1740
PspN4I GGNNCC 7 cut(s) 438, 633, 1045, 1097, 1537, 1710, 1935
PspPI GGNCC 5 cut(s) 308, 554, 1095, 1708, 1933
PspPPI RGGWCCY 1 cut(s) 1933
PstI CTGCAG 2 cut(s) 72, 1431
PsuI RGATCY 2 cut(s) 262, 667
PvuII CAGCTG 2 cut(s) 395, 1348
RsaI GTAC 7 cut(s) 447, 633, 860, 926, 1331, 1549, 2007
RsaNI GTAC 7 cut(s) 446, 632, 859, 925, 1330, 1548, 2006
SacI GAGCTC 1 cut(s) 867
SapI GCTCTTC 1 cut(s) 1314
SaqAI TTAA 6 cut(s) 86, 684, 707, 1355, 1911, 1989
Sau3AI GATC 6 cut(s) 262, 667, 1018, 1175, 1792, 1841
Sau96I GGNCC 5 cut(s) 308, 554, 1095, 1708, 1933
ScaI AGTACT 2 cut(s) 447, 1331
SchI GAGTC 4 cut(s) 153, 756, 814, 1231
ScrFI CCNGG 3 cut(s) 1055, 1211, 1742
SduI GDGCHC 3 cut(s) 496, 867, 1423
SfaNI GCATC 5 cut(s) 128, 646, 1680, 1705, 1876
SfcI CTRYAG 3 cut(s) 68, 573, 1427
SinI GGWCC 3 cut(s) 554, 1095, 1933
SmlI CTYRAG 3 cut(s) 85, 335, 1910
SmoI CTYRAG 3 cut(s) 85, 335, 1910
SpeI ACTAGT 1 cut(s) 448
SsiI CCGC 4 cut(s) 306, 934, 1481, 1638
SspI AATATT 1 cut(s) 704
SspMI CTAG 4 cut(s) 449, 512, 837, 1271
SstI GAGCTC 1 cut(s) 867
StyD4I CCNGG 3 cut(s) 1053, 1209, 1740
StyI CCWWGG 3 cut(s) 894, 966, 1937
TaaI ACNGT 3 cut(s) 445, 532, 574
TaiI ACGT 1 cut(s) 60
TaqI TCGA 3 cut(s) 808, 1603, 1884
TaqII GACCGA 2 cut(s) 810, 2014
TatI WGTACW 4 cut(s) 445, 1329, 1547, 2005
TauI GCSGC 2 cut(s) 309, 1640
TfiI GAWTC 3 cut(s) 64, 1767, 2065
Tru1I TTAA 6 cut(s) 86, 684, 707, 1355, 1911, 1989
Tru9I TTAA 6 cut(s) 86, 684, 707, 1355, 1911, 1989
TscAI CASTG 1 cut(s) 102
TseFI GTSAC 3 cut(s) 1312, 1412, 1595
TseI GCWGC 9 cut(s) 197, 392, 395, 724, 1142, 1345, 1426, 1693, 1847
Tsp45I GTSAC 3 cut(s) 1312, 1412, 1595
TspDTI ATGAA 6 cut(s) 410, 579, 862, 1169, 1287, 2078
TspRI CASTG 1 cut(s) 102
Van91I CCANNNNNTGG 1 cut(s) 650
Vha464I CTTAAG 2 cut(s) 85, 1910
VpaK11BI GGWCC 3 cut(s) 554, 1095, 1933
XapI RAATTY 3 cut(s) 811, 1266, 1432
XbaI TCTAGA 1 cut(s) 511
XceI RCATGY 3 cut(s) 718, 1893, 2012
XcmI CCANNNNNNNNNTGG 2 cut(s) 901, 964
XmiI GTMKAC 4 cut(s) 1290, 1567, 1894, 1996
XmnI GAANNNNTTC 1 cut(s) 1488
XspI CTAG 4 cut(s) 449, 512, 837, 1271
ZrmI AGTACT 2 cut(s) 447, 1331
Zsp2I ATGCAT 1 cut(s) 1891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.